Rw5G034200

Domain of unknown function (DUF296)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0011861
Physical Location & Seq
Reverse (-)
58801388 .. 58804427
3040 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G034200.1

Sequence Viewer

Length: 1083 bp
ATGGAAGAGAAGAACATTATGGAATCTCCCCCTATCTCTCCCCTAAGCAGTGAACCGGAACCGAACAATAGTAAACAGGTGGTGGGGTGTCTTCCACAGTTTGACCTTATCGCCAATGAGTTGGTGGAGTTTCCTCCACAGTCTAACCCTATCGCCGATGAGTTGGTGGAGTCTTCTCTGCAGTCTGATCCCATTGCCAATGAGTTGTTGGACTCTCCTCCACAATCTGAGTCTGACCCCATCGCCGATGAGAACAATGAAGCACTATCAGCTGAAGGCGGTCAATCGGAGGCAGGTGAAGTGATGATGACACCTAAGAAGAAGAGGGGGAGACCTAGGAATTATGAGGTCAATGAAGAAATGGTCTCTGATGTTCCGGCTTCCAACTCGGAGGGCTCCTCTAAGAAAGCAAAAGACCACCCCAAAGGCTCAGGCAATAAATTGCAACGCCCCGCTGCCGATGAAACTGGTGGAGCGGGAAATAACTTCATCCCTTTCGCAGTGACTGTGCAGACTGGGGAGGACCTTGTTCATAAAATATGGGTCATGGCACAAAGGGACCATCAATCAATTTGCATTCTTTCGGCTACTGGTGCTGTTTCTAAACCTGTCATACGACCTAATGCAGCTGTAGGGTATGTAAAATACGAGGGTTGTTATGATATCATCAATCTCTCTGGATCGTTTGTATACCATGCAATGTATCCACACAAAAAATTGGGCATGATAACTATTCTATTGGCTAATCCCGATAATACAGTTTTTGGTGGCTCTGTTTTATCAGGAGCACCGCTCATAGCTGGGAGTCCTATTCAACTCATTCTCGGAGCTTTTGATCAAGACATACATGAAGAGTTGAATGAGAAAAGGAGGCCCTCAGTCGCATCTTCTACATACACGAGTGTAACAGCTGGTCCTGACCTTGCAAGGATTCCTGCACAAGTGATGCCGATCCAAGCAGTAGATCGGGATGAAACTCGTATTCAATATCGACGAATATCTCCATATTTCAACAACAGTCGGGATGGGACTTGTGTTCAATATCGACCAATACCTCCGTATTTCAACAACAGTCTTCCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AT_hook DUF296

Protein Analysis

360

Amino Acids

39.1

Weight (kDa)

4.9

Isoelectric Point (pI)

53.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PCC PF03479 165 - 279 1e-13 Plants and Prokaryotes Conserved (PCC) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 284
Acc36I ACCTGC 1 cut(s) 284
AccBSI CCGCTC 2 cut(s) 476, 793
AccI GTMKAC 1 cut(s) 690
AciI CCGC 4 cut(s) 279, 453, 476, 791
AclWI GGATC 3 cut(s) 182, 688, 946
AcuI CTGAAG 1 cut(s) 294
AgsI TTSAA 6 cut(s) 815, 859, 986, 1012, 1040, 1066
AluBI AGCT 5 cut(s) 272, 629, 800, 830, 911
AluI AGCT 5 cut(s) 272, 629, 800, 830, 911
Alw21I GWGCWC 1 cut(s) 790
Alw26I GTCTC 2 cut(s) 325, 370
AlwI GGATC 3 cut(s) 182, 688, 946
AlwNI CAGNNNCTG 1 cut(s) 506
AoxI GGCC 1 cut(s) 872
ApeKI GCWGC 2 cut(s) 455, 626
AspA2I CCTAGG 1 cut(s) 335
AspS9I GGNCC 4 cut(s) 523, 559, 873, 914
AsuHPI GGTGA 1 cut(s) 308
AvaII GGWCC 3 cut(s) 523, 559, 914
AvrII CCTAGG 1 cut(s) 335
BanII GRGCYC 1 cut(s) 398
BauI CACGAG 1 cut(s) 898
BbsI GAAGAC 3 cut(s) 83, 165, 1067
Bbv12I GWGCWC 1 cut(s) 790
BbvI GCAGC 2 cut(s) 442, 638
BccI CCATC 3 cut(s) 248, 570, 1019
BciVI GTATCC 1 cut(s) 714
BclI TGATCA 1 cut(s) 835
BcoDI GTCTC 2 cut(s) 325, 370
BfaI CTAG 1 cut(s) 336
BfmI CTRYAG 2 cut(s) 179, 630
BfuAI ACCTGC 1 cut(s) 284
BfuI GTATCC 1 cut(s) 714
BisI GCNGC 2 cut(s) 456, 627
BlnI CCTAGG 1 cut(s) 335
BlsI GCNGC 2 cut(s) 457, 628
Bme18I GGWCC 3 cut(s) 523, 559, 914
BmgT120I GGNCC 4 cut(s) 523, 559, 873, 914
BmiI GGNNCC 3 cut(s) 60, 397, 560
BmrI ACTGGG 1 cut(s) 525
BmsI GCATC 2 cut(s) 893, 936
BmuI ACTGGG 1 cut(s) 525
BpiI GAAGAC 3 cut(s) 83, 165, 1067
BplI GAGNNNNNCTC 4 cut(s) 383, 415, 777, 809
Bpu10I CCTNAGC 2 cut(s) 44, 430
BsaBI GATNNNNATC 1 cut(s) 950
BsaI GGTCTC 2 cut(s) 325, 370
BsaJI CCNNGG 1 cut(s) 335
BsaWI WCCGGW 1 cut(s) 55
Bse1I ACTGG 3 cut(s) 472, 520, 595
Bse3DI GCAATG 2 cut(s) 192, 705
Bse8I GATNNNNATC 1 cut(s) 950
BseDI CCNNGG 1 cut(s) 335
BseGI GGATG 3 cut(s) 489, 976, 1030
BseJI GATNNNNATC 1 cut(s) 950
BseMI GCAATG 2 cut(s) 192, 705
BseMII CTCAG 3 cut(s) 219, 444, 891
BseNI ACTGG 3 cut(s) 472, 520, 595
BseRI GAGGAG 2 cut(s) 207, 388
BseXI GCAGC 2 cut(s) 442, 638
BseYI CCCAGC 1 cut(s) 800
BsgI GTGCAG 2 cut(s) 530, 921
BshFI GGCC 1 cut(s) 874
BsiHKAI GWGCWC 1 cut(s) 790
BsiSI CCGG 2 cut(s) 56, 377
BslFI GGGAC 2 cut(s) 572, 1042
BsmAI GTCTC 2 cut(s) 325, 370
BsmFI GGGAC 2 cut(s) 572, 1042
BsmI GAATGC 1 cut(s) 576
BsnI GGCC 1 cut(s) 874
Bso31I GGTCTC 2 cut(s) 325, 370
Bsp1286I GDGCHC 2 cut(s) 398, 790
Bsp143I GATC 5 cut(s) 187, 680, 835, 951, 964
BspACI CCGC 4 cut(s) 279, 453, 476, 791
BspANI GGCC 1 cut(s) 874
BspCNI CTCAG 3 cut(s) 220, 443, 890
BspLI GGNNCC 3 cut(s) 60, 397, 560
BspMAI CTGCAG 1 cut(s) 183
BspMI ACCTGC 1 cut(s) 284
BspPI GGATC 3 cut(s) 182, 688, 946
BspTNI GGTCTC 2 cut(s) 325, 370
BsrBI CCGCTC 2 cut(s) 476, 793
BsrDI GCAATG 2 cut(s) 192, 705
BsrI ACTGG 3 cut(s) 472, 520, 595
BssECI CCNNGG 1 cut(s) 335
BssMI GATC 5 cut(s) 187, 680, 835, 951, 964
BssNAI GTATAC 1 cut(s) 691
BssSI CACGAG 1 cut(s) 898
BssT1I CCWWGG 1 cut(s) 335
Bst1107I GTATAC 1 cut(s) 691
Bst2BI CACGAG 1 cut(s) 898
Bst4CI ACNGT 6 cut(s) 99, 141, 508, 760, 1019, 1073
Bst6I CTCTTC 2 cut(s) 317, 846
BstDEI CTNAG 7 cut(s) 44, 228, 315, 402, 430, 877, 1080
BstF5I GGATG 3 cut(s) 489, 976, 1030
BstKTI GATC 5 cut(s) 190, 683, 838, 954, 967
BstMAI GTCTC 2 cut(s) 325, 370
BstMBI GATC 5 cut(s) 187, 680, 835, 951, 964
BstMWI GCNNNNNNNGC 2 cut(s) 269, 593
BstSFI CTRYAG 2 cut(s) 179, 630
BstV1I GCAGC 2 cut(s) 442, 638
BstV2I GAAGAC 3 cut(s) 83, 165, 1067
BstXI CCANNNNNNTGG 1 cut(s) 121
BstZ17I GTATAC 1 cut(s) 691
BsuI GTATCC 1 cut(s) 714
BsuRI GGCC 1 cut(s) 874
BtgZI GCGATG 1 cut(s) 226
BtsCI GGATG 3 cut(s) 489, 976, 1030
BtsI GCAGTG 2 cut(s) 55, 507
BtsIMutI CAGTG 2 cut(s) 55, 507
BveI ACCTGC 1 cut(s) 284
CaiI CAGNNNCTG 1 cut(s) 506
Cfr13I GGNCC 4 cut(s) 523, 559, 873, 914
CviAII CATG 4 cut(s) 547, 695, 724, 848
DdeI CTNAG 7 cut(s) 44, 228, 315, 402, 430, 877, 1080
DpnI GATC 5 cut(s) 189, 682, 837, 953, 966
DpnII GATC 5 cut(s) 187, 680, 835, 951, 964
Eam1104I CTCTTC 2 cut(s) 317, 846
EarI CTCTTC 2 cut(s) 317, 846
Eco130I CCWWGG 1 cut(s) 335
Eco24I GRGCYC 1 cut(s) 398
Eco31I GGTCTC 2 cut(s) 325, 370
Eco32I GATATC 1 cut(s) 664
Eco47I GGWCC 3 cut(s) 523, 559, 914
Eco57I CTGAAG 1 cut(s) 294
EcoO109I RGGNCCY 2 cut(s) 523, 873
EcoRV GATATC 1 cut(s) 664
EcoT14I CCWWGG 1 cut(s) 335
EcoT38I GRGCYC 1 cut(s) 398
ErhI CCWWGG 1 cut(s) 335
FaeI CATG 4 cut(s) 550, 698, 727, 851
FaqI GGGAC 2 cut(s) 572, 1042
FatI CATG 4 cut(s) 546, 694, 723, 847
FauI CCCGC 2 cut(s) 460, 469
FbaI TGATCA 1 cut(s) 835
FblI GTMKAC 1 cut(s) 690
Fnu4HI GCNGC 2 cut(s) 456, 627
FokI GGATG 3 cut(s) 476, 983, 1037
FriOI GRGCYC 1 cut(s) 398
Fsp4HI GCNGC 2 cut(s) 456, 627
FspBI CTAG 1 cut(s) 336
GluI GCNGC 2 cut(s) 456, 627
GsaI CCCAGC 1 cut(s) 804
HaeIII GGCC 1 cut(s) 874
HapII CCGG 2 cut(s) 56, 377
Hin1II CATG 4 cut(s) 550, 698, 727, 851
HinfI GANTC 6 cut(s) 23, 170, 212, 230, 805, 931
HpaII CCGG 2 cut(s) 56, 377
HphI GGTGA 1 cut(s) 308
Hpy166II GTNNAC 3 cut(s) 53, 74, 691
Hpy188I TCNGA 7 cut(s) 187, 229, 235, 289, 370, 391, 827
Hpy188III TCNNGA 7 cut(s) 678, 749, 783, 839, 917, 968, 1022
Hpy8I GTNNAC 3 cut(s) 53, 74, 691
Hpy99I CGWCG 1 cut(s) 996
HpyAV CCTTC 1 cut(s) 269
HpyCH4III ACNGT 6 cut(s) 99, 141, 508, 760, 1019, 1073
HpyCH4V TGCA 8 cut(s) 181, 445, 511, 576, 626, 698, 926, 938
HpyF10VI GCNNNNNNNGC 2 cut(s) 269, 593
HpyF3I CTNAG 7 cut(s) 44, 228, 315, 402, 430, 877, 1080
Hsp92II CATG 4 cut(s) 550, 698, 727, 851
Ksp22I TGATCA 1 cut(s) 835
Kzo9I GATC 5 cut(s) 187, 680, 835, 951, 964
LmnI GCTCC 4 cut(s) 401, 473, 785, 827
Lsp1109I GCAGC 2 cut(s) 442, 638
LweI GCATC 2 cut(s) 893, 936
MaeI CTAG 1 cut(s) 336
MaeIII GTNAC 2 cut(s) 502, 904
MalI GATC 5 cut(s) 189, 682, 837, 953, 966
MbiI CCGCTC 2 cut(s) 476, 793
MboI GATC 5 cut(s) 187, 680, 835, 951, 964
MhlI GDGCHC 2 cut(s) 398, 790
MluCI AATT 4 cut(s) 340, 440, 570, 716
MlyI GAGTC 4 cut(s) 179, 206, 239, 814
MmeI TCCRAC 2 cut(s) 189, 408
MspA1I CMGCKG 4 cut(s) 272, 455, 629, 911
MspI CCGG 2 cut(s) 56, 377
Mva1269I GAATGC 1 cut(s) 576
MwoI GCNNNNNNNGC 2 cut(s) 269, 593
NdeII GATC 5 cut(s) 187, 680, 835, 951, 964
NlaIII CATG 4 cut(s) 550, 698, 727, 851
NlaIV GGNNCC 3 cut(s) 60, 397, 560
NmuCI GTSAC 1 cut(s) 502
PaqCI CACCTGC 1 cut(s) 284
PctI GAATGC 1 cut(s) 576
PfeI GAWTC 2 cut(s) 23, 931
PkrI GCNGC 2 cut(s) 457, 628
PleI GAGTC 4 cut(s) 178, 206, 238, 813
PpsI GAGTC 4 cut(s) 178, 206, 238, 813
PpuMI RGGWCCY 1 cut(s) 523
Psp5II RGGWCCY 1 cut(s) 523
PspFI CCCAGC 1 cut(s) 800
PspN4I GGNNCC 3 cut(s) 60, 397, 560
PspPI GGNCC 4 cut(s) 523, 559, 873, 914
PspPPI RGGWCCY 1 cut(s) 523
PstI CTGCAG 1 cut(s) 183
PstNI CAGNNNCTG 1 cut(s) 506
PvuII CAGCTG 3 cut(s) 272, 629, 911
SatI GCNGC 2 cut(s) 456, 627
Sau3AI GATC 5 cut(s) 187, 680, 835, 951, 964
Sau96I GGNCC 4 cut(s) 523, 559, 873, 914
SchI GAGTC 4 cut(s) 179, 206, 239, 814
SduI GDGCHC 2 cut(s) 398, 790
SfaNI GCATC 2 cut(s) 893, 936
SfcI CTRYAG 2 cut(s) 179, 630
SinI GGWCC 3 cut(s) 523, 559, 914
Sse9I AATT 4 cut(s) 340, 440, 570, 716
SsiI CCGC 4 cut(s) 279, 453, 476, 791
SspMI CTAG 1 cut(s) 336
StyI CCWWGG 1 cut(s) 335
TaaI ACNGT 6 cut(s) 99, 141, 508, 760, 1019, 1073
TaqI TCGA 2 cut(s) 991, 1045
TasI AATT 4 cut(s) 340, 440, 570, 716
TfiI GAWTC 2 cut(s) 23, 931
TscAI CASTG 2 cut(s) 55, 507
TseFI GTSAC 1 cut(s) 502
TseI GCWGC 2 cut(s) 455, 626
Tsp45I GTSAC 1 cut(s) 502
TspDTI ATGAA 7 cut(s) 273, 369, 477, 478, 521, 864, 987
TspGWI ACGGA 1 cut(s) 1047
TspRI CASTG 2 cut(s) 55, 507
VpaK11BI GGWCC 3 cut(s) 523, 559, 914
XcmI CCANNNNNNNNNTGG 2 cut(s) 121, 205
XmaJI CCTAGG 1 cut(s) 335
XmiI GTMKAC 1 cut(s) 690
XspI CTAG 1 cut(s) 336
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.