AT2G46110

Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is transferred onto alpha-ketoisovalerate to form ketopantoate

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0006569
Physical Location & Seq
Reverse (-)
18954570 .. 18956582
2013 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G46110.1

Sequence Viewer

Length: 1044 bp
ATGGCGTCCTCACTCACTAGGAATTGCTCTCGCTTCTCAAAAGCCATCTCTGTCCGTTTCATGAGTAACCTACCGGAAAACACCGTCTACGGAGGACCAAAGCCACAAAACCCAAACCAAAGAGTGACGCTAACGCATCTCAGACAAAAACACCGGAGAGGAGAACCGATCACCGTCGTAACTGCCTACGATTACCCATCCGCCGTTCATTTAGACACAGCTGGTATCGATGTTTGTCTTGTCGGTGACTCAGCTTCCATGGTTGTTCATGGTCACGACACAACACTTCCTATCTCCTTAGACGAGATGCTTGTTCATTGTCGTGCCGTTGCTCGAGGCGCTAAACGACCTCTCCTAGTTGGTGACTTGCCATTTGGTACCTACGAGTCAAGCTCTAGTCAGGCGGTTGATACTGCGGTTAGGGTTTTGAAGGAAGGAGGAATGGATGCGATTAAGCTCGAAGGAGGATCAGCTTCTAGGATCACTGCTGCAAAAGCTATAGTTGAAGCAGGAATTGCGGTTATTGGTCATGTGGGTCTAACCCCTCAGGCGATTAGCGTTCTTGGTGGTTTCAGACCCCAAGGCAGAAACATAGCTAGTGCTGTTAAAGTTGTTGAGACTGCAATGGCTTTACAAGAAGCTGGATGTTTTTCGGTTGTTTTGGAATGCGTTCCACCTCCTGTAGCTGCTGCTGCAACTTCTGCCCTCAAGATACCAACCATTGGCATTGGGGCTGGACCTTTCTGCAGTGGACAGGTGCTAGTTTACCATGATCTTCTTGGTATGATGCAACATCCACATCATGCCAAGGTTACTCCAAAGTTTTGCAAGCAGTACGCTAATGTCGGAGAAGTGATTAACAAAGCCCTAATGGAATACAAAGAAGAAGTCTCTAAAAAAGTGTTTCCAGGTCCTTCTCACAGTCCTTACAAGATCACAGCAAGTGAGCTCGATGGGTTCTTAACCGAGTTGCAGAAGCTTGGTTTTGACAAGGCTGCTTCTGCAGCAGCTTTAGCAGCTGAGAACATGGAGCCTTCAAAGTGA

Protein Analysis

347

Amino Acids

36.69

Weight (kDa)

8.6

Isoelectric Point (pI)

35.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pantoate_transf PF02548 41 - 304 7.4e-106 Ketopantoate hydroxymethyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 377
AccB1I GGYRCC 1 cut(s) 377
AccB7I CCANNNNNTGG 1 cut(s) 722
AccI GTMKAC 1 cut(s) 87
AciI CCGC 4 cut(s) 201, 404, 416, 518
AclWI GGATC 2 cut(s) 475, 488
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 2 cut(s) 379, 836
AfiI CCNNNNNNNGG 1 cut(s) 722
AgsI TTSAA 3 cut(s) 430, 506, 1038
AjnI CCWGG 1 cut(s) 907
Alw21I GWGCWC 1 cut(s) 951
Alw26I GTCTC 2 cut(s) 611, 895
AlwI GGATC 2 cut(s) 475, 488
Ama87I CYCGRG 1 cut(s) 333
ApeKI GCWGC 8 cut(s) 488, 686, 689, 692, 995, 1004, 1007, 1016
ArsI GACNNNNNNTTYG 4 cut(s) 356, 388, 873, 905
Asp700I GAANNNNTTC 1 cut(s) 669
Asp718I GGTACC 1 cut(s) 377
AspLEI GCGC 1 cut(s) 341
AspS9I GGNCC 3 cut(s) 95, 737, 911
AsuHPI GGTGA 3 cut(s) 163, 257, 374
AvaI CYCGRG 1 cut(s) 333
AvaII GGWCC 3 cut(s) 95, 737, 911
AxyI CCTNAGG 1 cut(s) 546
BaeI ACNNNNGTAYC 2 cut(s) 402, 435
BanI GGYRCC 1 cut(s) 377
BanII GRGCYC 1 cut(s) 951
Bbv12I GWGCWC 1 cut(s) 951
BbvI GCAGC 8 cut(s) 475, 673, 676, 679, 982, 1016, 1019, 1028
BccI CCATC 3 cut(s) 53, 205, 947
BceAI ACGGC 2 cut(s) 188, 311
BciT130I CCWGG 1 cut(s) 909
BcoDI GTCTC 2 cut(s) 611, 895
BfaI CTAG 6 cut(s) 18, 356, 396, 477, 597, 761
BfmI CTRYAG 4 cut(s) 498, 681, 745, 1002
BfoI RGCGCY 1 cut(s) 342
BisI GCNGC 8 cut(s) 489, 687, 690, 693, 996, 1005, 1008, 1017
BlsI GCNGC 8 cut(s) 490, 688, 691, 694, 997, 1006, 1009, 1018
Bme1390I CCNGG 1 cut(s) 909
Bme18I GGWCC 3 cut(s) 95, 737, 911
BmeT110I CYCGRG 1 cut(s) 333
BmgT120I GGNCC 3 cut(s) 95, 737, 911
BmiI GGNNCC 2 cut(s) 379, 1032
BmrFI CCNGG 1 cut(s) 909
BmsI GCATC 4 cut(s) 145, 297, 436, 777
BplI GAGNNNNNCTC 2 cut(s) 377, 409
BpuEI CTTGAG 1 cut(s) 692
Bsa29I ATCGAT 1 cut(s) 228
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 3 cut(s) 258, 580, 807
BsaWI WCCGGW 2 cut(s) 73, 153
BsaXI ACNNNNNCTCC 2 cut(s) 336, 366
Bsc4I CCNNNNNNNGG 1 cut(s) 722
Bse21I CCTNAGG 1 cut(s) 546
Bse3DI GCAATG 1 cut(s) 630
BseBI CCWGG 1 cut(s) 909
BseCI ATCGAT 1 cut(s) 228
BseDI CCNNGG 3 cut(s) 258, 580, 807
BseGI GGATG 4 cut(s) 197, 451, 650, 793
BseLI CCNNNNNNNGG 1 cut(s) 722
BseMI GCAATG 1 cut(s) 630
BseMII CTCAG 4 cut(s) 154, 264, 560, 1011
BseRI GAGGAG 1 cut(s) 174
BseXI GCAGC 8 cut(s) 475, 673, 676, 679, 982, 1016, 1019, 1028
BshNI GGYRCC 1 cut(s) 377
BshVI ATCGAT 1 cut(s) 228
BsiHKAI GWGCWC 1 cut(s) 951
BsiHKCI CYCGRG 1 cut(s) 333
BsiSI CCGG 2 cut(s) 74, 154
BslI CCNNNNNNNGG 1 cut(s) 722
BsmAI GTCTC 2 cut(s) 611, 895
BsmI GAATGC 1 cut(s) 671
BsoBI CYCGRG 1 cut(s) 333
Bsp1286I GDGCHC 1 cut(s) 951
Bsp143I GATC 5 cut(s) 168, 467, 480, 772, 933
Bsp19I CCATGG 1 cut(s) 258
BspACI CCGC 4 cut(s) 201, 404, 416, 518
BspCNI CTCAG 4 cut(s) 153, 263, 559, 1012
BspDI ATCGAT 1 cut(s) 228
BspHI TCATGA 1 cut(s) 60
BspLI GGNNCC 2 cut(s) 379, 1032
BspMAI CTGCAG 2 cut(s) 749, 1006
BspPI GGATC 2 cut(s) 475, 488
BspT107I GGYRCC 1 cut(s) 377
BsrDI GCAATG 1 cut(s) 630
BssECI CCNNGG 3 cut(s) 258, 580, 807
BssMI GATC 5 cut(s) 168, 467, 480, 772, 933
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 3 cut(s) 258, 580, 807
Bst2UI CCWGG 1 cut(s) 909
Bst4CI ACNGT 3 cut(s) 85, 175, 923
BstACI GRCGYC 1 cut(s) 5
BstAPI GCANNNNNTGC 2 cut(s) 515, 701
BstC8I GCNNGC 1 cut(s) 830
BstDEI CTNAG 5 cut(s) 140, 250, 298, 546, 1020
BstDSI CCRYGG 1 cut(s) 258
BstF5I GGATG 4 cut(s) 197, 451, 650, 793
BstH2I RGCGCY 1 cut(s) 342
BstHHI GCGC 1 cut(s) 341
BstKTI GATC 5 cut(s) 171, 470, 483, 775, 936
BstMAI GTCTC 2 cut(s) 611, 895
BstMBI GATC 5 cut(s) 168, 467, 480, 772, 933
BstMWI GCNNNNNNNGC 9 cut(s) 338, 494, 515, 692, 701, 1001, 1004, 1013, 1016
BstNI CCWGG 1 cut(s) 909
BstSCI CCNGG 1 cut(s) 907
BstSFI CTRYAG 4 cut(s) 498, 681, 745, 1002
BstV1I GCAGC 8 cut(s) 475, 673, 676, 679, 982, 1016, 1019, 1028
Bsu15I ATCGAT 1 cut(s) 228
Bsu36I CCTNAGG 1 cut(s) 546
BsuTUI ATCGAT 1 cut(s) 228
BtgI CCRYGG 1 cut(s) 258
BtsCI GGATG 4 cut(s) 197, 451, 650, 793
BtsI GCAGTG 2 cut(s) 483, 754
BtsIMutI CAGTG 2 cut(s) 483, 754
Cac8I GCNNGC 1 cut(s) 830
CciI TCATGA 1 cut(s) 60
CfoI GCGC 1 cut(s) 341
Cfr13I GGNCC 3 cut(s) 95, 737, 911
ClaI ATCGAT 1 cut(s) 228
CseI GACGC 1 cut(s) 136
Csp6I GTAC 2 cut(s) 378, 835
CviAII CATG 7 cut(s) 61, 259, 269, 530, 770, 803, 1027
CviQI GTAC 2 cut(s) 378, 835
DdeI CTNAG 5 cut(s) 140, 250, 298, 546, 1020
DpnI GATC 5 cut(s) 170, 469, 482, 774, 935
DpnII GATC 5 cut(s) 168, 467, 480, 772, 933
EciI GGCGGA 1 cut(s) 190
Ecl136II GAGCTC 1 cut(s) 949
Eco130I CCWWGG 3 cut(s) 258, 580, 807
Eco24I GRGCYC 1 cut(s) 951
Eco47I GGWCC 3 cut(s) 95, 737, 911
Eco53kI GAGCTC 1 cut(s) 949
Eco81I CCTNAGG 1 cut(s) 546
Eco88I CYCGRG 1 cut(s) 333
EcoICRI GAGCTC 1 cut(s) 949
EcoO109I RGGNCCY 1 cut(s) 911
EcoRII CCWGG 1 cut(s) 907
EcoT14I CCWWGG 3 cut(s) 258, 580, 807
EcoT38I GRGCYC 1 cut(s) 951
ErhI CCWWGG 3 cut(s) 258, 580, 807
FaeI CATG 7 cut(s) 64, 262, 272, 533, 773, 806, 1030
FatI CATG 7 cut(s) 60, 258, 268, 529, 769, 802, 1026
FblI GTMKAC 1 cut(s) 87
Fnu4HI GCNGC 8 cut(s) 489, 687, 690, 693, 996, 1005, 1008, 1017
FokI GGATG 4 cut(s) 184, 458, 657, 780
FriOI GRGCYC 1 cut(s) 951
Fsp4HI GCNGC 8 cut(s) 489, 687, 690, 693, 996, 1005, 1008, 1017
FspBI CTAG 6 cut(s) 18, 356, 396, 477, 597, 761
GlaI GCGC 1 cut(s) 340
GluI GCNGC 8 cut(s) 489, 687, 690, 693, 996, 1005, 1008, 1017
HaeII RGCGCY 1 cut(s) 342
HapII CCGG 2 cut(s) 74, 154
HgaI GACGC 1 cut(s) 136
HhaI GCGC 1 cut(s) 341
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 7 cut(s) 64, 262, 272, 533, 773, 806, 1030
Hin6I GCGC 1 cut(s) 339
HinP1I GCGC 1 cut(s) 339
HindIII AAGCTT 1 cut(s) 977
HinfI GANTC 2 cut(s) 248, 386
HpaII CCGG 2 cut(s) 74, 154
HphI GGTGA 3 cut(s) 163, 257, 374
Hpy166II GTNNAC 3 cut(s) 88, 752, 766
Hpy188I TCNGA 3 cut(s) 143, 575, 848
Hpy188III TCNNGA 3 cut(s) 61, 275, 709
Hpy8I GTNNAC 3 cut(s) 88, 752, 766
Hpy99I CGWCG 1 cut(s) 179
HpyAV CCTTC 5 cut(s) 424, 428, 455, 924, 1044
HpyCH4III ACNGT 3 cut(s) 85, 175, 923
HpyCH4V TGCA 8 cut(s) 491, 623, 695, 747, 790, 828, 973, 1004
HpyF10VI GCNNNNNNNGC 9 cut(s) 338, 494, 515, 692, 701, 1001, 1004, 1013, 1016
HpyF3I CTNAG 5 cut(s) 140, 250, 298, 546, 1020
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 7 cut(s) 64, 262, 272, 533, 773, 806, 1030
HspAI GCGC 1 cut(s) 339
KpnI GGTACC 1 cut(s) 381
Kzo9I GATC 5 cut(s) 168, 467, 480, 772, 933
LmnI GCTCC 1 cut(s) 1030
Lsp1109I GCAGC 8 cut(s) 475, 673, 676, 679, 982, 1016, 1019, 1028
LweI GCATC 4 cut(s) 145, 297, 436, 777
MaeI CTAG 6 cut(s) 18, 356, 396, 477, 597, 761
MaeIII GTNAC 7 cut(s) 65, 124, 178, 245, 272, 362, 811
MalI GATC 5 cut(s) 170, 469, 482, 774, 935
MboI GATC 5 cut(s) 168, 467, 480, 772, 933
MboII GAAGA 2 cut(s) 767, 896
MhlI GDGCHC 1 cut(s) 951
MluCI AATT 2 cut(s) 22, 513
MlyI GAGTC 2 cut(s) 242, 395
MmeI TCCRAC 1 cut(s) 826
MroXI GAANNNNTTC 1 cut(s) 669
MseI TTAA 4 cut(s) 453, 606, 858, 962
MslI CAYNNNNRTG 1 cut(s) 321
MspA1I CMGCKG 2 cut(s) 221, 1019
MspI CCGG 2 cut(s) 74, 154
MspR9I CCNGG 1 cut(s) 909
Mva1269I GAATGC 1 cut(s) 671
MvaI CCWGG 1 cut(s) 909
MwoI GCNNNNNNNGC 9 cut(s) 338, 494, 515, 692, 701, 1001, 1004, 1013, 1016
NcoI CCATGG 1 cut(s) 258
NdeII GATC 5 cut(s) 168, 467, 480, 772, 933
NlaIII CATG 7 cut(s) 64, 262, 272, 533, 773, 806, 1030
NlaIV GGNNCC 2 cut(s) 379, 1032
NmuCI GTSAC 4 cut(s) 124, 245, 272, 362
PaeR7I CTCGAG 1 cut(s) 333
PagI TCATGA 1 cut(s) 60
PctI GAATGC 1 cut(s) 671
PdmI GAANNNNTTC 1 cut(s) 669
PflMI CCANNNNNTGG 1 cut(s) 722
PkrI GCNGC 8 cut(s) 490, 688, 691, 694, 997, 1006, 1009, 1018
PleI GAGTC 2 cut(s) 242, 394
PpsI GAGTC 2 cut(s) 242, 394
PpuMI RGGWCCY 1 cut(s) 911
Psp124BI GAGCTC 1 cut(s) 951
Psp5II RGGWCCY 1 cut(s) 911
Psp6I CCWGG 1 cut(s) 907
PspGI CCWGG 1 cut(s) 907
PspN4I GGNNCC 2 cut(s) 379, 1032
PspPI GGNCC 3 cut(s) 95, 737, 911
PspPPI RGGWCCY 1 cut(s) 911
PspXI VCTCGAGB 1 cut(s) 333
PstI CTGCAG 2 cut(s) 749, 1006
PvuII CAGCTG 2 cut(s) 221, 1019
RsaI GTAC 2 cut(s) 379, 836
RsaNI GTAC 2 cut(s) 378, 835
RseI CAYNNNNRTG 1 cut(s) 321
SacI GAGCTC 1 cut(s) 951
SaqAI TTAA 4 cut(s) 453, 606, 858, 962
SatI GCNGC 8 cut(s) 489, 687, 690, 693, 996, 1005, 1008, 1017
Sau3AI GATC 5 cut(s) 168, 467, 480, 772, 933
Sau96I GGNCC 3 cut(s) 95, 737, 911
SchI GAGTC 2 cut(s) 242, 395
ScrFI CCNGG 1 cut(s) 909
SduI GDGCHC 1 cut(s) 951
SfaNI GCATC 4 cut(s) 145, 297, 436, 777
SfcI CTRYAG 4 cut(s) 498, 681, 745, 1002
Sfr274I CTCGAG 1 cut(s) 333
SinI GGWCC 3 cut(s) 95, 737, 911
SlaI CTCGAG 1 cut(s) 333
SmiMI CAYNNNNRTG 1 cut(s) 321
SmlI CTYRAG 2 cut(s) 333, 707
SmoI CTYRAG 2 cut(s) 333, 707
Sse9I AATT 2 cut(s) 22, 513
SsiI CCGC 4 cut(s) 201, 404, 416, 518
SspMI CTAG 6 cut(s) 18, 356, 396, 477, 597, 761
SstI GAGCTC 1 cut(s) 951
StyD4I CCNGG 1 cut(s) 907
StyI CCWWGG 3 cut(s) 258, 580, 807
TaaI ACNGT 3 cut(s) 85, 175, 923
TaqI TCGA 4 cut(s) 228, 334, 459, 951
TasI AATT 2 cut(s) 22, 513
Tru1I TTAA 4 cut(s) 453, 606, 858, 962
Tru9I TTAA 4 cut(s) 453, 606, 858, 962
TscAI CASTG 2 cut(s) 490, 754
TseFI GTSAC 4 cut(s) 124, 245, 272, 362
TseI GCWGC 8 cut(s) 488, 686, 689, 692, 995, 1004, 1007, 1016
Tsp45I GTSAC 4 cut(s) 124, 245, 272, 362
TspDTI ATGAA 4 cut(s) 49, 197, 257, 305
TspGWI ACGGA 2 cut(s) 44, 105
TspRI CASTG 2 cut(s) 490, 754
Van91I CCANNNNNTGG 1 cut(s) 722
VpaK11BI GGWCC 3 cut(s) 95, 737, 911
XcmI CCANNNNNNNNNTGG 1 cut(s) 776
XhoI CTCGAG 1 cut(s) 333
XmiI GTMKAC 1 cut(s) 87
XmnI GAANNNNTTC 1 cut(s) 669
XspI CTAG 6 cut(s) 18, 356, 396, 477, 597, 761
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.