Rw1G024250

Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is transferred onto alpha-ketoisovalerate to form ketopantoate

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0006569
Physical Location & Seq
Reverse (-)
50508772 .. 50510822
2051 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G024250.1

Sequence Viewer

Length: 1038 bp
ATGGCATTCACGGCCGCCGCTTTCGTCTCCCCGCCCGGACTCGTCGTCCGCTGCATGAACAGCGTGCCTGACAGCACAGTCTACGGCGGCCCAAAGTCGCAAAGCCCAGACCGGAGAGTCACCGTCACTCAAATAAAGCAGAAGCACAGCAAGGGCGAGCCCATCACCGCCGTCACCGCCTACAACTACCCCTCGGCGGTGCACTTGGACTCGGCCGGGATAGACATTGCCGTGGTCGGCGACTCGGCCGCGATGGTTGTTCACGGATATGATACCACTCTCCCCATAACGCTGGAGGAAATGCTCGTCCACTGCCGAGCGGTGGCGCGTGGGGCCAAGCACCCGCTTTTGGTTGCGGACTTGCCGTTCGGATTCTACGAGTCTAGCTCCATTCAGGCTATCGATTCAGCGATGAGAATTTTAAAGGAAGGCGCAATGGATGCAGTAAAGTTGGAAGCAGGGGCACCTTCAAGAATTACTGCGGCAAAAGCTATTGTTGAAGCAGGAATTGCTGTGATGGGGCATGTAGGACTTACTCCTCAGGCTATTAGCGTTCTCGGGGGTTTTAGGCCACAAGGAAGGAATGTGGCTAGTGCTGTAAAGATTGTGGAGACAGCGCTGGCTTTGCAAGAAGCCGGGTGCTTTGCAATTGTATTGGAATGCGTGCCACCACTAGTGGCAGCTGCTGCAACATCTGCTCTTCAGATTCCTACAATTGGCATTGGAGCAGGGTCTTTTTGCAGTGGGCAGGTGCTAGTTTACCATGATCTTCTTGGAATGATGCAGCACCCACGTCATGCAAAGGTTACCCCAAAATTCTGTAAGCAATATGCACATGTTGGAAACGTCATCAACAAAGCTCTAGTAGACTACAAGGAAGAAGTCACCAATGGTGCGTTTCCAGGCGCTTTCCATAGCCCATATAAAATCAATGCTGCTGATGTTGATGGCTTTGTTAGTGAGTTAGGAAGGATGGGTTTGGACAAGGCAGCATCTGCGGCGGCTGAAGTAGCTGACAAGAATAATACAAGCAAATGA

Protein Analysis

345

Amino Acids

35.98

Weight (kDa)

7.16

Isoelectric Point (pI)

29.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pantoate_transf PF02548 39 - 302 3.4e-102 Ketopantoate hydroxymethyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 739
AasI GACNNNNNNGTC 2 cut(s) 77, 116
Acc36I ACCTGC 1 cut(s) 739
AccB1I GGYRCC 1 cut(s) 463
AccBSI CCGCTC 1 cut(s) 320
AccI GTMKAC 2 cut(s) 81, 867
AccII CGCG 2 cut(s) 251, 328
AcoI YGGCCR 3 cut(s) 12, 213, 246
AcsI RAATTY 2 cut(s) 417, 815
AcuI CTGAAG 2 cut(s) 686, 1026
AfeI AGCGCT 1 cut(s) 618
AfiI CCNNNNNNNGG 4 cut(s) 196, 322, 349, 716
AflIII ACRYGT 1 cut(s) 835
AgsI TTSAA 2 cut(s) 471, 500
AhdI GACNNNNNGTC 1 cut(s) 44
AhlI ACTAGT 1 cut(s) 673
AjiI CACGTC 1 cut(s) 794
AjnI CCWGG 1 cut(s) 901
AloI GAACNNNNNNTCC 2 cut(s) 350, 382
AluBI AGCT 5 cut(s) 387, 491, 683, 860, 1013
AluI AGCT 5 cut(s) 387, 491, 683, 860, 1013
Alw21I GWGCWC 1 cut(s) 204
Alw26I GTCTC 2 cut(s) 31, 605
Alw44I GTGCAC 1 cut(s) 200
AlwNI CAGNNNCTG 2 cut(s) 686, 995
Ama87I CYCGRG 1 cut(s) 557
Aor51HI AGCGCT 1 cut(s) 618
AoxI GGCC 6 cut(s) 12, 88, 213, 246, 333, 569
ApaLI GTGCAC 1 cut(s) 200
ApeKI GCWGC 7 cut(s) 51, 680, 683, 686, 784, 935, 989
ApoI RAATTY 2 cut(s) 417, 815
ArsI GACNNNNNNTTYG 2 cut(s) 350, 382
AspLEI GCGC 4 cut(s) 328, 434, 619, 908
AspS9I GGNCC 2 cut(s) 89, 333
AsuC2I CCSGG 3 cut(s) 36, 217, 637
AsuHPI GGTGA 4 cut(s) 112, 157, 166, 877
AvaI CYCGRG 1 cut(s) 557
AxyI CCTNAGG 1 cut(s) 540
BaeGI GKGCMC 2 cut(s) 204, 466
BanI GGYRCC 1 cut(s) 463
BanII GRGCYC 1 cut(s) 162
Bbv12I GWGCWC 1 cut(s) 204
BbvI GCAGC 7 cut(s) 38, 670, 673, 692, 796, 922, 1001
BccI CCATC 5 cut(s) 170, 247, 511, 941, 967
BceAI ACGGC 5 cut(s) 27, 100, 155, 215, 349
BciT130I CCWGG 1 cut(s) 903
BcnI CCSGG 3 cut(s) 36, 217, 637
BcoDI GTCTC 2 cut(s) 31, 605
BcuI ACTAGT 1 cut(s) 673
BfaI CTAG 5 cut(s) 384, 591, 674, 755, 863
BfoI RGCGCY 2 cut(s) 620, 909
BfuAI ACCTGC 1 cut(s) 739
Bme1390I CCNGG 4 cut(s) 36, 217, 637, 903
BmeRI GACNNNNNGTC 1 cut(s) 44
BmeT110I CYCGRG 1 cut(s) 557
BmgBI CACGTC 1 cut(s) 794
BmgT120I GGNCC 2 cut(s) 89, 333
BmiI GGNNCC 2 cut(s) 334, 465
BmrFI CCNGG 4 cut(s) 36, 217, 637, 903
BmsI GCATC 3 cut(s) 430, 771, 1001
BplI GAGNNNNNCTC 2 cut(s) 371, 403
BpmI CTGGAG 1 cut(s) 314
BpuMI CCSGG 3 cut(s) 36, 217, 637
Bsa29I ATCGAT 1 cut(s) 402
BsaJI CCNNGG 2 cut(s) 192, 231
BsaWI WCCGGW 1 cut(s) 111
Bsc4I CCNNNNNNNGG 4 cut(s) 196, 322, 349, 716
Bse21I CCTNAGG 1 cut(s) 540
Bse3DI GCAATG 2 cut(s) 225, 441
BseBI CCWGG 1 cut(s) 903
BseCI ATCGAT 1 cut(s) 402
BseDI CCNNGG 2 cut(s) 192, 231
BseGI GGATG 2 cut(s) 445, 978
BseLI CCNNNNNNNGG 4 cut(s) 196, 322, 349, 716
BseMI GCAATG 2 cut(s) 225, 441
BseMII CTCAG 1 cut(s) 554
BseRI GAGGAG 1 cut(s) 528
BseSI GKGCMC 2 cut(s) 204, 466
BseX3I CGGCCG 3 cut(s) 12, 213, 246
BseXI GCAGC 7 cut(s) 38, 670, 673, 692, 796, 922, 1001
Bsh1236I CGCG 2 cut(s) 251, 328
Bsh1285I CGRYCG 3 cut(s) 15, 216, 249
BshFI GGCC 6 cut(s) 14, 90, 215, 248, 335, 571
BshNI GGYRCC 1 cut(s) 463
BshVI ATCGAT 1 cut(s) 402
BsiEI CGRYCG 3 cut(s) 15, 216, 249
BsiHKAI GWGCWC 1 cut(s) 204
BsiHKCI CYCGRG 1 cut(s) 557
BsiSI CCGG 4 cut(s) 36, 112, 216, 636
BslI CCNNNNNNNGG 4 cut(s) 196, 322, 349, 716
BsmAI GTCTC 2 cut(s) 31, 605
BsmBI CGTCTC 1 cut(s) 31
BsmI GAATGC 2 cut(s) 5, 665
BsnI GGCC 6 cut(s) 14, 90, 215, 248, 335, 571
BsoBI CYCGRG 1 cut(s) 557
Bsp1286I GDGCHC 3 cut(s) 162, 204, 466
Bsp143I GATC 1 cut(s) 766
BspANI GGCC 6 cut(s) 14, 90, 215, 248, 335, 571
BspCNI CTCAG 1 cut(s) 553
BspDI ATCGAT 1 cut(s) 402
BspFNI CGCG 2 cut(s) 251, 328
BspLI GGNNCC 2 cut(s) 334, 465
BspMI ACCTGC 1 cut(s) 739
BspQI GCTCTTC 1 cut(s) 705
BspT107I GGYRCC 1 cut(s) 463
BsrBI CCGCTC 1 cut(s) 320
BsrDI GCAATG 2 cut(s) 225, 441
BssECI CCNNGG 2 cut(s) 192, 231
BssMI GATC 1 cut(s) 766
Bst2UI CCWGG 1 cut(s) 903
Bst4CI ACNGT 2 cut(s) 79, 124
Bst6I CTCTTC 1 cut(s) 705
BstAPI GCANNNNNTGC 5 cut(s) 440, 509, 686, 695, 995
BstC8I GCNNGC 4 cut(s) 65, 158, 621, 665
BstDEI CTNAG 1 cut(s) 540
BstDSI CCRYGG 1 cut(s) 231
BstEII GGTNACC 1 cut(s) 805
BstF5I GGATG 2 cut(s) 445, 978
BstFNI CGCG 2 cut(s) 251, 328
BstH2I RGCGCY 2 cut(s) 620, 909
BstHHI GCGC 4 cut(s) 328, 434, 619, 908
BstKTI GATC 1 cut(s) 769
BstMAI GTCTC 2 cut(s) 31, 605
BstMBI GATC 1 cut(s) 766
BstMCI CGRYCG 3 cut(s) 15, 216, 249
BstNI CCWGG 1 cut(s) 903
BstNSI RCATGY 2 cut(s) 527, 839
BstPI GGTNACC 1 cut(s) 805
BstSCI CCNGG 4 cut(s) 34, 215, 635, 901
BstSLI GKGCMC 2 cut(s) 204, 466
BstUI CGCG 2 cut(s) 251, 328
BstV1I GCAGC 7 cut(s) 38, 670, 673, 692, 796, 922, 1001
BstXI CCANNNNNNTGG 1 cut(s) 292
BstZI CGGCCG 3 cut(s) 12, 213, 246
Bsu15I ATCGAT 1 cut(s) 402
Bsu36I CCTNAGG 1 cut(s) 540
BsuRI GGCC 6 cut(s) 14, 90, 215, 248, 335, 571
BsuTUI ATCGAT 1 cut(s) 402
BtgI CCRYGG 1 cut(s) 231
BtgZI GCGATG 2 cut(s) 266, 425
BtrI CACGTC 1 cut(s) 794
BtsCI GGATG 2 cut(s) 445, 978
BtsI GCAGTG 2 cut(s) 310, 748
BtsIMutI CAGTG 2 cut(s) 310, 748
BveI ACCTGC 1 cut(s) 739
Cac8I GCNNGC 4 cut(s) 65, 158, 621, 665
CaiI CAGNNNCTG 2 cut(s) 686, 995
CfoI GCGC 4 cut(s) 328, 434, 619, 908
Cfr13I GGNCC 2 cut(s) 89, 333
ClaI ATCGAT 1 cut(s) 402
CviAII CATG 5 cut(s) 55, 524, 764, 797, 836
DdeI CTNAG 1 cut(s) 540
DpnI GATC 1 cut(s) 768
DpnII GATC 1 cut(s) 766
DraI TTTAAA 1 cut(s) 423
DrdI GACNNNNNNGTC 2 cut(s) 77, 116
DriI GACNNNNNGTC 1 cut(s) 44
DseDI GACNNNNNNGTC 2 cut(s) 77, 116
EaeI YGGCCR 3 cut(s) 12, 213, 246
EagI CGGCCG 3 cut(s) 12, 213, 246
Eam1104I CTCTTC 1 cut(s) 705
Eam1105I GACNNNNNGTC 1 cut(s) 44
EarI CTCTTC 1 cut(s) 705
EclXI CGGCCG 3 cut(s) 12, 213, 246
Eco24I GRGCYC 1 cut(s) 162
Eco47III AGCGCT 1 cut(s) 618
Eco52I CGGCCG 3 cut(s) 12, 213, 246
Eco57I CTGAAG 2 cut(s) 686, 1026
Eco81I CCTNAGG 1 cut(s) 540
Eco88I CYCGRG 1 cut(s) 557
Eco91I GGTNACC 1 cut(s) 805
EcoO65I GGTNACC 1 cut(s) 805
EcoRII CCWGG 1 cut(s) 901
EcoT38I GRGCYC 1 cut(s) 162
Esp3I CGTCTC 1 cut(s) 31
FaeI CATG 5 cut(s) 58, 527, 767, 800, 839
FatI CATG 5 cut(s) 54, 523, 763, 796, 835
FauI CCCGC 2 cut(s) 39, 351
FblI GTMKAC 2 cut(s) 81, 867
FokI GGATG 2 cut(s) 452, 985
FriOI GRGCYC 1 cut(s) 162
FspBI CTAG 5 cut(s) 384, 591, 674, 755, 863
GlaI GCGC 4 cut(s) 327, 433, 618, 907
GsuI CTGGAG 1 cut(s) 314
HaeII RGCGCY 2 cut(s) 620, 909
HaeIII GGCC 6 cut(s) 14, 90, 215, 248, 335, 571
HapII CCGG 4 cut(s) 36, 112, 216, 636
HhaI GCGC 4 cut(s) 328, 434, 619, 908
Hin1II CATG 5 cut(s) 58, 527, 767, 800, 839
Hin6I GCGC 4 cut(s) 326, 432, 617, 906
HinP1I GCGC 4 cut(s) 326, 432, 617, 906
HinfI GANTC 8 cut(s) 39, 117, 209, 242, 372, 380, 404, 706
HpaII CCGG 4 cut(s) 36, 112, 216, 636
HphI GGTGA 4 cut(s) 112, 157, 166, 877
Hpy166II GTNNAC 6 cut(s) 82, 202, 262, 310, 760, 868
Hpy188I TCNGA 2 cut(s) 371, 705
Hpy188III TCNNGA 1 cut(s) 471
Hpy8I GTNNAC 6 cut(s) 82, 202, 262, 310, 760, 868
Hpy99I CGWCG 1 cut(s) 47
HpyAV CCTTC 4 cut(s) 422, 477, 573, 963
HpyCH4III ACNGT 2 cut(s) 79, 124
HpyCH4IV ACGT 2 cut(s) 793, 846
HpyF3I CTNAG 1 cut(s) 540
HpySE526I ACGT 2 cut(s) 793, 846
Hsp92II CATG 5 cut(s) 58, 527, 767, 800, 839
HspAI GCGC 4 cut(s) 326, 432, 617, 906
Kzo9I GATC 1 cut(s) 766
LguI GCTCTTC 1 cut(s) 705
LmnI GCTCC 2 cut(s) 392, 725
Lsp1109I GCAGC 7 cut(s) 38, 670, 673, 692, 796, 922, 1001
LweI GCATC 3 cut(s) 430, 771, 1001
MaeI CTAG 5 cut(s) 384, 591, 674, 755, 863
MaeII ACGT 2 cut(s) 793, 846
MaeIII GTNAC 5 cut(s) 118, 124, 172, 805, 883
MalI GATC 1 cut(s) 768
MbiI CCGCTC 1 cut(s) 320
MboI GATC 1 cut(s) 766
MboII GAAGA 3 cut(s) 692, 761, 890
MfeI CAATTG 2 cut(s) 648, 714
MhlI GDGCHC 3 cut(s) 162, 204, 466
MluCI AATT 6 cut(s) 417, 474, 507, 648, 714, 815
MlyI GAGTC 5 cut(s) 33, 126, 203, 236, 389
MmeI TCCRAC 2 cut(s) 432, 820
MnlI CCTC 3 cut(s) 202, 289, 549
MseI TTAA 1 cut(s) 422
MslI CAYNNNNRTG 2 cut(s) 230, 267
MspA1I CMGCKG 2 cut(s) 51, 683
MspI CCGG 4 cut(s) 36, 112, 216, 636
MspR9I CCNGG 4 cut(s) 36, 217, 637, 903
MunI CAATTG 2 cut(s) 648, 714
Mva1269I GAATGC 2 cut(s) 5, 665
MvaI CCWGG 1 cut(s) 903
MvnI CGCG 2 cut(s) 251, 328
NciI CCSGG 3 cut(s) 36, 217, 637
NdeII GATC 1 cut(s) 766
NlaIII CATG 5 cut(s) 58, 527, 767, 800, 839
NlaIV GGNNCC 2 cut(s) 334, 465
NmeAIII GCCGAG 4 cut(s) 173, 191, 224, 341
NmuCI GTSAC 4 cut(s) 118, 124, 172, 883
NspI RCATGY 2 cut(s) 527, 839
PaqCI CACCTGC 1 cut(s) 739
PciI ACATGT 1 cut(s) 835
PciSI GCTCTTC 1 cut(s) 705
PcsI WCGNNNNNNNCGW 1 cut(s) 375
PctI GAATGC 2 cut(s) 5, 665
PfeI GAWTC 3 cut(s) 372, 404, 706
PleI GAGTC 5 cut(s) 33, 125, 203, 236, 388
PpsI GAGTC 5 cut(s) 33, 125, 203, 236, 388
PscI ACATGT 1 cut(s) 835
Psp6I CCWGG 1 cut(s) 901
PspEI GGTNACC 1 cut(s) 805
PspGI CCWGG 1 cut(s) 901
PspN4I GGNNCC 2 cut(s) 334, 465
PspPI GGNCC 2 cut(s) 89, 333
PstNI CAGNNNCTG 2 cut(s) 686, 995
PvuII CAGCTG 1 cut(s) 683
RseI CAYNNNNRTG 2 cut(s) 230, 267
SapI GCTCTTC 1 cut(s) 705
SaqAI TTAA 1 cut(s) 422
Sau3AI GATC 1 cut(s) 766
Sau96I GGNCC 2 cut(s) 89, 333
SchI GAGTC 5 cut(s) 33, 126, 203, 236, 389
ScrFI CCNGG 4 cut(s) 36, 217, 637, 903
SduI GDGCHC 3 cut(s) 162, 204, 466
SfaNI GCATC 3 cut(s) 430, 771, 1001
SmiMI CAYNNNNRTG 2 cut(s) 230, 267
SpeI ACTAGT 1 cut(s) 673
Sse9I AATT 6 cut(s) 417, 474, 507, 648, 714, 815
SspMI CTAG 5 cut(s) 384, 591, 674, 755, 863
StyD4I CCNGG 4 cut(s) 34, 215, 635, 901
TaaI ACNGT 2 cut(s) 79, 124
TaiI ACGT 2 cut(s) 796, 849
TaqI TCGA 1 cut(s) 402
TasI AATT 6 cut(s) 417, 474, 507, 648, 714, 815
TauI GCSGC 7 cut(s) 17, 20, 90, 251, 485, 1001, 1004
TfiI GAWTC 3 cut(s) 372, 404, 706
Tru1I TTAA 1 cut(s) 422
Tru9I TTAA 1 cut(s) 422
TscAI CASTG 2 cut(s) 317, 748
TseFI GTSAC 4 cut(s) 118, 124, 172, 883
TseI GCWGC 7 cut(s) 51, 680, 683, 686, 784, 935, 989
Tsp45I GTSAC 4 cut(s) 118, 124, 172, 883
TspDTI ATGAA 1 cut(s) 71
TspGWI ACGGA 1 cut(s) 279
TspRI CASTG 2 cut(s) 317, 748
VneI GTGCAC 1 cut(s) 200
XapI RAATTY 2 cut(s) 417, 815
XceI RCATGY 2 cut(s) 527, 839
XcmI CCANNNNNNNNNTGG 1 cut(s) 770
XmiI GTMKAC 2 cut(s) 81, 867
XspI CTAG 5 cut(s) 384, 591, 674, 755, 863
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.