AT2G46940

endonuclease activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0010914
Physical Location & Seq
Reverse (-)
19286434 .. 19287590
1157 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G46940.1

Sequence Viewer

Length: 759 bp
ATGGGGTCTGAAGAGAGAATGATGATAAGTTCTTCTTCAGTTCTAGACGAAGAGTATGAATATACAGATGATGGGTTTCATTACGAGACAAGGCAAAACAGTTTGTCTAGATTATCTATCTGCACAAACTCCTTCCACGAAGACGACGAAGATCATCCTTCAGAATTGGGTAATTTCTTCTCTACGTTGTCGTTAGAGAGCTTCAACGACGACGTCGGAGCCGAAGCTGACGTAGAAATCTCCGATGACGGTGGCGAATCGGATTCCGACAAAGACTCTTCCGGATTTTACTCTCTACCGACGATCACATCTCGCCGGAGAAGAAAACTCACAGTTTCCGGCGGTTTAGAAACGAGCAAAGAGAGTGAGACGAAAGTCGATGGGAGTAAGTGTAATATAGTTAGTCAACGGAGGGTATTAAGGGAAAAGAGTAAAAGAGGGAATCAGTACGGTCACTGGTTTAACGGCGTGGAGAGAGACAGTGACGGCGGAGGAGAGTTGACGGTGTTAACGAAGGTGAGGGGAGGGAAGAAGAAGTCAATGAAGATGGGGTTTGAAGAAGTCAAAGCTTGTAGAGATCTCGGCTTCGAACTGGATGTTCCGGGTCGGGTTTCAGGGTCAAACCGTGAAACTAGTAGTGGCGGCAACTCTCCCATTGCCAACTGGCGTATCTCAAGTCCTGGGGATGATCCGAAAGAGGTTAAGGCGAGATTAAAGATGTGGGCACAAGCAGTAGCTTTAGCTTCTGCGTCACGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

252

Amino Acids

27.79

Weight (kDa)

5.07

Isoelectric Point (pI)

50.21

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010914)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G46940 AT3G62070 AT4G01670
fragaria_vesca FvH4_1g26130
malus_domestica MD00G1137300.v1.1
prunus_persica Prupe.3G001400_v2.0.a1
pyrus_communis pycom03g07920
rosa_chinensis RchiOBHm_Chr3g0485631
rosa_laevigata RLG00000023140
rosa_multiflora Rmu_sc0001272.1_g000004 Rmu_sc0004711.1_g000006 Rmu_sc0036906.1_g000001
rosa_roxburghii Rroxscaffold_6G00396570 Rroxscaffold_6G00396880
rosa_rugosa Rorug03G0220300
rosa_samantha Rh3AG269000 Rh3BG304600 Rh3CG303200 Rh3DG299500
rosa_wichuraiana Rw3G023990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 216
AccIII TCCGGA 1 cut(s) 281
AciI CCGC 3 cut(s) 342, 489, 642
AclWI GGATC 1 cut(s) 683
AcuI CTGAAG 3 cut(s) 21, 30, 144
AcyI GRCGYC 1 cut(s) 213
AfaI GTAC 1 cut(s) 449
AgsI TTSAA 2 cut(s) 205, 557
AhlI ACTAGT 1 cut(s) 632
AjnI CCWGG 1 cut(s) 679
AluBI AGCT 5 cut(s) 201, 227, 569, 737, 743
AluI AGCT 5 cut(s) 201, 227, 569, 737, 743
Alw26I GTCTC 3 cut(s) 80, 362, 471
AlwI GGATC 1 cut(s) 683
Aor13HI TCCGGA 1 cut(s) 281
AsuC2I CCSGG 1 cut(s) 603
AsuHPI GGTGA 1 cut(s) 529
AsuII TTCGAA 1 cut(s) 588
BaeGI GKGCMC 1 cut(s) 727
BaeI ACNNNNGTAYC 2 cut(s) 652, 685
BbsI GAAGAC 1 cut(s) 147
BccI CCATC 3 cut(s) 65, 374, 541
BceAI ACGGC 2 cut(s) 481, 502
BciT130I CCWGG 1 cut(s) 681
BcnI CCSGG 1 cut(s) 603
BcoDI GTCTC 3 cut(s) 80, 362, 471
BcuI ACTAGT 1 cut(s) 632
BfaI CTAG 3 cut(s) 44, 108, 633
BglII AGATCT 1 cut(s) 577
BisI GCNGC 1 cut(s) 643
BlsI GCNGC 1 cut(s) 644
Bme1390I CCNGG 2 cut(s) 603, 681
BmiI GGNNCC 1 cut(s) 220
BmrFI CCNGG 2 cut(s) 603, 681
BoxI GACNNNNGTC 1 cut(s) 374
BpiI GAAGAC 1 cut(s) 147
Bpu14I TTCGAA 1 cut(s) 588
BpuEI CTTGAG 1 cut(s) 658
BpuMI CCSGG 1 cut(s) 603
BsaHI GRCGYC 1 cut(s) 213
BsaJI CCNNGG 1 cut(s) 680
BsaWI WCCGGW 1 cut(s) 281
BsaXI ACNNNNNCTCC 2 cut(s) 376, 406
Bse1I ACTGG 3 cut(s) 461, 597, 668
Bse3DI GCAATG 1 cut(s) 654
BseAI TCCGGA 1 cut(s) 281
BseBI CCWGG 1 cut(s) 681
BseDI CCNNGG 1 cut(s) 680
BseGI GGATG 3 cut(s) 154, 601, 691
BseMI GCAATG 1 cut(s) 654
BseNI ACTGG 3 cut(s) 461, 597, 668
BseRI GAGGAG 1 cut(s) 507
BseSI GKGCMC 1 cut(s) 727
BsgI GTGCAG 1 cut(s) 106
BsiSI CCGG 4 cut(s) 282, 316, 339, 602
BsmAI GTCTC 3 cut(s) 80, 362, 471
BsmBI CGTCTC 1 cut(s) 362
Bsp119I TTCGAA 1 cut(s) 588
Bsp1286I GDGCHC 1 cut(s) 727
Bsp13I TCCGGA 1 cut(s) 281
Bsp143I GATC 4 cut(s) 151, 303, 577, 688
BspACI CCGC 3 cut(s) 342, 489, 642
BspEI TCCGGA 1 cut(s) 281
BspLI GGNNCC 1 cut(s) 220
BspPI GGATC 1 cut(s) 683
BspT104I TTCGAA 1 cut(s) 588
BsrDI GCAATG 1 cut(s) 654
BsrI ACTGG 3 cut(s) 461, 597, 668
BssECI CCNNGG 1 cut(s) 680
BssMI GATC 4 cut(s) 151, 303, 577, 688
BssNI GRCGYC 1 cut(s) 213
Bst2UI CCWGG 1 cut(s) 681
Bst4CI ACNGT 7 cut(s) 101, 251, 334, 452, 482, 505, 626
Bst6I CTCTTC 3 cut(s) 6, 45, 283
BstACI GRCGYC 1 cut(s) 213
BstBI TTCGAA 1 cut(s) 588
BstF5I GGATG 3 cut(s) 154, 601, 691
BstKTI GATC 4 cut(s) 154, 306, 580, 691
BstMAI GTCTC 3 cut(s) 80, 362, 471
BstMBI GATC 4 cut(s) 151, 303, 577, 688
BstNI CCWGG 1 cut(s) 681
BstPAI GACNNNNGTC 1 cut(s) 374
BstSCI CCNGG 2 cut(s) 601, 679
BstSLI GKGCMC 1 cut(s) 727
BstV2I GAAGAC 1 cut(s) 147
BstX2I RGATCY 1 cut(s) 577
BstYI RGATCY 1 cut(s) 577
BtsCI GGATG 3 cut(s) 154, 601, 691
BtsIMutI CAGTG 2 cut(s) 454, 487
CseI GACGC 1 cut(s) 738
Csp6I GTAC 1 cut(s) 448
CviJI RGCY 7 cut(s) 201, 221, 227, 569, 585, 737, 743
CviKI_1 RGCY 7 cut(s) 201, 221, 227, 569, 585, 737, 743
CviQI GTAC 1 cut(s) 448
DpnI GATC 4 cut(s) 153, 305, 579, 690
DpnII GATC 4 cut(s) 151, 303, 577, 688
Eam1104I CTCTTC 3 cut(s) 6, 45, 283
EarI CTCTTC 3 cut(s) 6, 45, 283
EciI GGCGGA 1 cut(s) 504
Eco57I CTGAAG 3 cut(s) 21, 30, 144
EcoRII CCWGG 1 cut(s) 679
Esp3I CGTCTC 1 cut(s) 362
FaiI YATR 3 cut(s) 57, 63, 398
FalI AAGNNNNNCTT 2 cut(s) 19, 51
Fnu4HI GCNGC 1 cut(s) 643
FokI GGATG 3 cut(s) 141, 608, 698
Fsp4HI GCNGC 1 cut(s) 643
FspBI CTAG 3 cut(s) 44, 108, 633
GluI GCNGC 1 cut(s) 643
HapII CCGG 4 cut(s) 282, 316, 339, 602
HgaI GACGC 1 cut(s) 738
Hin1I GRCGYC 1 cut(s) 213
HincII GTYRAC 3 cut(s) 407, 501, 510
HindII GTYRAC 3 cut(s) 407, 501, 510
HindIII AAGCTT 1 cut(s) 567
HinfI GANTC 4 cut(s) 257, 263, 275, 442
HpaI GTTAAC 1 cut(s) 510
HpaII CCGG 4 cut(s) 282, 316, 339, 602
HphI GGTGA 1 cut(s) 529
Hpy166II GTNNAC 3 cut(s) 407, 501, 510
Hpy188I TCNGA 7 cut(s) 10, 163, 218, 244, 262, 268, 693
Hpy188III TCNNGA 3 cut(s) 44, 108, 282
Hpy8I GTNNAC 3 cut(s) 407, 501, 510
Hpy99I CGWCG 5 cut(s) 149, 212, 215, 218, 304
HpyAV CCTTC 3 cut(s) 142, 168, 508
HpyCH4III ACNGT 7 cut(s) 101, 251, 334, 452, 482, 505, 626
HpyCH4IV ACGT 4 cut(s) 185, 213, 231, 754
HpyCH4V TGCA 1 cut(s) 123
HpySE526I ACGT 4 cut(s) 185, 213, 231, 754
Hsp92I GRCGYC 1 cut(s) 213
Kpn2I TCCGGA 1 cut(s) 281
KspAI GTTAAC 1 cut(s) 510
Kzo9I GATC 4 cut(s) 151, 303, 577, 688
LmnI GCTCC 1 cut(s) 218
MaeI CTAG 3 cut(s) 44, 108, 633
MaeII ACGT 4 cut(s) 185, 213, 231, 754
MaeIII GTNAC 3 cut(s) 452, 482, 750
MalI GATC 4 cut(s) 153, 305, 579, 690
MboI GATC 4 cut(s) 151, 303, 577, 688
MflI RGATCY 1 cut(s) 577
MhlI GDGCHC 1 cut(s) 727
MluCI AATT 2 cut(s) 164, 172
MlyI GAGTC 1 cut(s) 269
MmeI TCCRAC 2 cut(s) 196, 291
MnlI CCTC 6 cut(s) 405, 431, 485, 513, 518, 691
MroI TCCGGA 1 cut(s) 281
MseI TTAA 6 cut(s) 419, 462, 509, 702, 713, 757
MspI CCGG 4 cut(s) 282, 316, 339, 602
MspR9I CCNGG 2 cut(s) 603, 681
MvaI CCWGG 1 cut(s) 681
NciI CCSGG 1 cut(s) 603
NdeII GATC 4 cut(s) 151, 303, 577, 688
NlaIV GGNNCC 1 cut(s) 220
NmeAIII GCCGAG 1 cut(s) 561
NmuCI GTSAC 3 cut(s) 452, 482, 750
NspV TTCGAA 1 cut(s) 588
PcsI WCGNNNNNNNCGW 3 cut(s) 144, 219, 509
PfeI GAWTC 3 cut(s) 257, 263, 442
PflFI GACNNNGTC 1 cut(s) 212
PkrI GCNGC 1 cut(s) 644
PleI GAGTC 1 cut(s) 269
PpsI GAGTC 1 cut(s) 269
PshAI GACNNNNGTC 1 cut(s) 374
Psp6I CCWGG 1 cut(s) 679
PspGI CCWGG 1 cut(s) 679
PspN4I GGNNCC 1 cut(s) 220
PsuI RGATCY 1 cut(s) 577
PsyI GACNNNGTC 1 cut(s) 212
RsaI GTAC 1 cut(s) 449
RsaNI GTAC 1 cut(s) 448
SaqAI TTAA 6 cut(s) 419, 462, 509, 702, 713, 757
SatI GCNGC 1 cut(s) 643
Sau3AI GATC 4 cut(s) 151, 303, 577, 688
SchI GAGTC 1 cut(s) 269
ScrFI CCNGG 2 cut(s) 603, 681
SduI GDGCHC 1 cut(s) 727
SfuI TTCGAA 1 cut(s) 588
SmlI CTYRAG 1 cut(s) 673
SmoI CTYRAG 1 cut(s) 673
SpeI ACTAGT 1 cut(s) 632
Sse9I AATT 2 cut(s) 164, 172
SsiI CCGC 3 cut(s) 342, 489, 642
SspMI CTAG 3 cut(s) 44, 108, 633
StyD4I CCNGG 2 cut(s) 601, 679
TaaI ACNGT 7 cut(s) 101, 251, 334, 452, 482, 505, 626
TaiI ACGT 4 cut(s) 188, 216, 234, 757
TaqI TCGA 2 cut(s) 378, 588
TasI AATT 2 cut(s) 164, 172
TauI GCSGC 1 cut(s) 645
TfiI GAWTC 3 cut(s) 257, 263, 442
Tru1I TTAA 6 cut(s) 419, 462, 509, 702, 713, 757
Tru9I TTAA 6 cut(s) 419, 462, 509, 702, 713, 757
TscAI CASTG 2 cut(s) 461, 487
TseFI GTSAC 3 cut(s) 452, 482, 750
Tsp45I GTSAC 3 cut(s) 452, 482, 750
TspDTI ATGAA 3 cut(s) 68, 72, 557
TspGWI ACGGA 1 cut(s) 424
TspRI CASTG 2 cut(s) 461, 487
Tth111I GACNNNGTC 1 cut(s) 212
XbaI TCTAGA 2 cut(s) 43, 107
XspI CTAG 3 cut(s) 44, 108, 633
ZraI GACGTC 1 cut(s) 214
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.