AT3G01015

TPX2 (targeting protein for Xklp2) protein family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0004726
Physical Location & Seq
Reverse (-)
1609 .. 4159
2551 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G01015.1

Sequence Viewer

Length: 1467 bp
ATGGAGTCGACGAATTTGAAGAATGCTAAACACGAGACTCTGGTGATGGATCCGATCAGTTTTCAATCCAAGGCTCAAGAGGTTTCACGATTCTCCGAGAATTCTAACCCTAACTTCGTCTCACACTCGACTCCACTCGAGAAATCGAGTAAATCTTCTGCGCAGAAGAATCCGAAATGGAAGCCTAATCCTGTTCCGGCGGTTTTCTCACCACGGAATCGGATCAGAGAGAGGAGGTTCGTGGTGGTGAAGAAGAATTCAAGGAAGGAGAAGAATGATTCCGCGTCTGTGGATTGCAAATGTGGAGCTAAGACCATTTCCAATATGAAGAAATGTGTCTGTATAGCATACGAAACTCTACGGGCGTCACAGGAAGAGTTCTTCAACAATCGAAGAGAATCCGTATCAGAAATTGGAGAATCCAGCCAGAATTTGGAGGATGGAAATGAACAAGTCGAATTTGGAGATTCTGATGAAACTAGGGTTTCTTTGATGAAGAGGAGAAGAGAAAAAGTATTGGAAGAAGCGAGAATGAGTATACCTGAATTTGGTAAAGTGATGCATCTAGTTAAAGCTTTTGAGAAGCTTACTTGCTTCCCACTTTCGAAGGTAACTTCTAAGGAAGAAGAAGATCAGATTAAGCAACCTTTGAAATGGGAATTGCCTGGAATGAGCCAGCCTAAGTGTTCAGAATCTGAGACAGATCAATTCACTTGGAGTTCTTCATTCTATCCATCATCTGGTTTGATCTTAACAGCTACGAATCTAGGACTAGAACAACCTCATGCCTCAGTTTCTTCATCATGGGACAATAGTGTTTCAAGTCTCAACTCAAATGGTGGCCGGAGGGGCAGAAGAAATAGCTTCGAATCCTCTGCTTCAATGGGAAGTAGAAGATCAACGAAGAAGCAGATCAAAGTCACTTCTCTGAAGCCATTTAAACTCAGAACTGAGGAACGGGGACGAATGAAAGAAGAAGAGTTTGCAAAGAAGCTACACGAGATGACGTTGGAGAAAGCAAAAAAGAGAATCCCCATAGCTCAAGGTCTTCCCTGGACAACTGATGAACCTGAGAATCTGGTTAAGCCTCATGTGAAAGATATCACGATACCAGTAGACTTAAAGCTCCATTCCGACATTCGAGCAGTAGAACGTGCAGAATTTGATTACCAGGTTACTGAGAAAATAAACCTAGTCGAGCAATACAAAACAGAAAGAGAACGCCAACAAAAGCTAGCAGAAGAAGAAGAGATACGAAGGCTGAGGAAAGAGTTGGTCCCTAAGGCACAACCAATGCCATACTTTGATCGACCATTCATTCCCAAAAGGTCGAACAAGCACCCGACGGTACCTCGAGATCCCAAGTTTAATATACCGCAACACAAGAAGATAAGATGCTGCAGTACATCGTCTTGGAGTGACACTGGATCTTACATGAGCGACCTCTTATACCAACAAGACCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPX2

Protein Analysis

488

Amino Acids

56.17

Weight (kDa)

9.35

Isoelectric Point (pI)

58.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPX2 PF06886 375 - 449 7.6e-27 Targeting protein for Xklp2 (TPX2) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 162
Acc65I GGTACC 1 cut(s) 1348
AccB1I GGYRCC 1 cut(s) 1348
AccB7I CCANNNNNTGG 2 cut(s) 433, 740
AccI GTMKAC 3 cut(s) 8, 538, 1116
AccII CGCG 1 cut(s) 284
AciI CCGC 3 cut(s) 200, 282, 1376
AclWI GGATC 5 cut(s) 44, 57, 230, 1352, 1435
AcoI YGGCCR 1 cut(s) 841
AcsI RAATTY 7 cut(s) 13, 100, 256, 430, 458, 545, 1160
AcuI CTGAAG 1 cut(s) 950
AcyI GRCGYC 1 cut(s) 365
AfaI GTAC 2 cut(s) 1350, 1405
AfiI CCNNNNNNNGG 3 cut(s) 433, 548, 740
AgsI TTSAA 7 cut(s) 19, 65, 261, 385, 652, 822, 882
AjnI CCWGG 3 cut(s) 664, 1052, 1170
AluBI AGCT 9 cut(s) 308, 575, 586, 758, 864, 994, 1040, 1126, 1234
AluI AGCT 9 cut(s) 308, 575, 586, 758, 864, 994, 1040, 1126, 1234
Alw26I GTCTC 4 cut(s) 29, 124, 692, 830
AlwI GGATC 5 cut(s) 44, 57, 230, 1352, 1435
AlwNI CAGNNNCTG 1 cut(s) 695
Ama87I CYCGRG 2 cut(s) 137, 1353
AoxI GGCC 1 cut(s) 841
ApeKI GCWGC 1 cut(s) 1398
ApoI RAATTY 7 cut(s) 13, 100, 256, 430, 458, 545, 1160
Asp718I GGTACC 1 cut(s) 1348
AspLEI GCGC 1 cut(s) 163
AspS9I GGNCC 1 cut(s) 1276
AsuHPI GGTGA 3 cut(s) 55, 201, 259
AsuII TTCGAA 2 cut(s) 605, 867
AsuNHI GCTAGC 1 cut(s) 1234
AvaI CYCGRG 2 cut(s) 137, 1353
AvaII GGWCC 1 cut(s) 1276
AxyI CCTNAGG 1 cut(s) 1281
BamHI GGATCC 1 cut(s) 49
BanI GGYRCC 1 cut(s) 1348
BarI GAAGNNNNNNTAC 2 cut(s) 1236, 1268
BauI CACGAG 2 cut(s) 32, 998
BbsI GAAGAC 1 cut(s) 1040
BbvCI CCTCAGC 1 cut(s) 1262
BbvI GCAGC 1 cut(s) 1385
BccI CCATC 3 cut(s) 40, 434, 742
BcgI CGANNNNNNTGC 2 cut(s) 857, 891
BciT130I CCWGG 3 cut(s) 666, 1054, 1172
BcoDI GTCTC 4 cut(s) 29, 124, 692, 830
BfaI CTAG 6 cut(s) 480, 566, 767, 773, 1193, 1235
BfmI CTRYAG 1 cut(s) 1399
BglI GCCNNNNNGGC 1 cut(s) 849
BisI GCNGC 1 cut(s) 1399
BlsI GCNGC 1 cut(s) 1400
Bme1390I CCNGG 3 cut(s) 666, 1054, 1172
Bme18I GGWCC 1 cut(s) 1276
BmeT110I CYCGRG 2 cut(s) 137, 1353
BmgT120I GGNCC 1 cut(s) 1276
BmiI GGNNCC 3 cut(s) 51, 1278, 1350
BmrFI CCNGG 3 cut(s) 666, 1054, 1172
BmsI GCATC 3 cut(s) 549, 571, 1385
BmtI GCTAGC 1 cut(s) 1238
BpiI GAAGAC 1 cut(s) 1040
Bpu10I CCTNAGC 1 cut(s) 1262
Bpu14I TTCGAA 2 cut(s) 605, 867
BpuEI CTTGAG 2 cut(s) 60, 1026
BsaHI GRCGYC 1 cut(s) 365
BsaJI CCNNGG 3 cut(s) 69, 212, 1052
Bsc4I CCNNNNNNNGG 3 cut(s) 433, 548, 740
Bse1I ACTGG 2 cut(s) 1112, 1429
Bse21I CCTNAGG 1 cut(s) 1281
BseBI CCWGG 3 cut(s) 666, 1054, 1172
BseDI CCNNGG 3 cut(s) 69, 212, 1052
BseGI GGATG 1 cut(s) 445
BseLI CCNNNNNNNGG 3 cut(s) 433, 548, 740
BseMII CTCAG 7 cut(s) 687, 804, 942, 958, 1062, 1170, 1253
BseNI ACTGG 2 cut(s) 1112, 1429
BseRI GAGGAG 2 cut(s) 247, 514
BseXI GCAGC 1 cut(s) 1385
BsgI GTGCAG 1 cut(s) 1176
Bsh1236I CGCG 1 cut(s) 284
BshFI GGCC 1 cut(s) 843
BshNI GGYRCC 1 cut(s) 1348
BsiHKCI CYCGRG 2 cut(s) 137, 1353
BsiSI CCGG 2 cut(s) 197, 844
BslFI GGGAC 3 cut(s) 821, 975, 1262
BslI CCNNNNNNNGG 3 cut(s) 433, 548, 740
BsmAI GTCTC 4 cut(s) 29, 124, 692, 830
BsmBI CGTCTC 1 cut(s) 124
BsmFI GGGAC 3 cut(s) 821, 975, 1262
BsmI GAATGC 1 cut(s) 28
BsnI GGCC 1 cut(s) 843
BsoBI CYCGRG 2 cut(s) 137, 1353
Bsp119I TTCGAA 2 cut(s) 605, 867
BspACI CCGC 3 cut(s) 200, 282, 1376
BspANI GGCC 1 cut(s) 843
BspCNI CTCAG 7 cut(s) 688, 803, 943, 957, 1063, 1171, 1254
BspFNI CGCG 1 cut(s) 284
BspLI GGNNCC 3 cut(s) 51, 1278, 1350
BspMAI CTGCAG 1 cut(s) 1403
BspOI GCTAGC 1 cut(s) 1238
BspPI GGATC 5 cut(s) 44, 57, 230, 1352, 1435
BspT104I TTCGAA 2 cut(s) 605, 867
BspT107I GGYRCC 1 cut(s) 1348
BsrI ACTGG 2 cut(s) 1112, 1429
BssECI CCNNGG 3 cut(s) 69, 212, 1052
BssNAI GTATAC 1 cut(s) 539
BssNI GRCGYC 1 cut(s) 365
BssSI CACGAG 2 cut(s) 32, 998
BssT1I CCWWGG 1 cut(s) 69
Bst1107I GTATAC 1 cut(s) 539
Bst2BI CACGAG 2 cut(s) 32, 998
Bst2UI CCWGG 3 cut(s) 666, 1054, 1172
Bst4CI ACNGT 1 cut(s) 1348
Bst6I CTCTTC 6 cut(s) 369, 388, 491, 499, 972, 1242
BstACI GRCGYC 1 cut(s) 365
BstBI TTCGAA 2 cut(s) 605, 867
BstC8I GCNNGC 2 cut(s) 677, 1236
BstDSI CCRYGG 1 cut(s) 212
BstF5I GGATG 1 cut(s) 445
BstFNI CGCG 1 cut(s) 284
BstHHI GCGC 1 cut(s) 163
BstMAI GTCTC 4 cut(s) 29, 124, 692, 830
BstMWI GCNNNNNNNGC 1 cut(s) 849
BstNI CCWGG 3 cut(s) 666, 1054, 1172
BstSCI CCNGG 3 cut(s) 664, 1052, 1170
BstSFI CTRYAG 1 cut(s) 1399
BstUI CGCG 1 cut(s) 284
BstV1I GCAGC 1 cut(s) 1385
BstV2I GAAGAC 1 cut(s) 1040
BstX2I RGATCY 3 cut(s) 49, 1357, 1427
BstYI RGATCY 3 cut(s) 49, 1357, 1427
BstZ17I GTATAC 1 cut(s) 539
Bsu36I CCTNAGG 1 cut(s) 1281
BsuRI GGCC 1 cut(s) 843
BtgI CCRYGG 1 cut(s) 212
BtsCI GGATG 1 cut(s) 445
BtsIMutI CAGTG 1 cut(s) 1422
Cac8I GCNNGC 2 cut(s) 677, 1236
CaiI CAGNNNCTG 1 cut(s) 695
CfoI GCGC 1 cut(s) 163
Cfr13I GGNCC 1 cut(s) 1276
CseI GACGC 2 cut(s) 273, 354
CsiI ACCWGGT 1 cut(s) 1170
Csp6I GTAC 2 cut(s) 1349, 1404
CviAII CATG 4 cut(s) 785, 804, 1091, 1435
CviQI GTAC 2 cut(s) 1349, 1404
DraI TTTAAA 1 cut(s) 940
EaeI YGGCCR 1 cut(s) 841
Eam1104I CTCTTC 6 cut(s) 369, 388, 491, 499, 972, 1242
EarI CTCTTC 6 cut(s) 369, 388, 491, 499, 972, 1242
Eco130I CCWWGG 1 cut(s) 69
Eco32I GATATC 1 cut(s) 1102
Eco47I GGWCC 1 cut(s) 1276
Eco57I CTGAAG 1 cut(s) 950
Eco81I CCTNAGG 1 cut(s) 1281
Eco88I CYCGRG 2 cut(s) 137, 1353
EcoRI GAATTC 2 cut(s) 100, 256
EcoRII CCWGG 3 cut(s) 664, 1052, 1170
EcoRV GATATC 1 cut(s) 1102
EcoT14I CCWWGG 1 cut(s) 69
EcoT22I ATGCAT 1 cut(s) 564
ErhI CCWWGG 1 cut(s) 69
Esp3I CGTCTC 1 cut(s) 124
FaeI CATG 4 cut(s) 788, 807, 1094, 1438
FaqI GGGAC 3 cut(s) 821, 975, 1262
FatI CATG 4 cut(s) 784, 803, 1090, 1434
FblI GTMKAC 3 cut(s) 8, 538, 1116
Fnu4HI GCNGC 1 cut(s) 1399
FokI GGATG 1 cut(s) 452
Fsp4HI GCNGC 1 cut(s) 1399
FspBI CTAG 6 cut(s) 480, 566, 767, 773, 1193, 1235
FspI TGCGCA 1 cut(s) 162
GlaI GCGC 1 cut(s) 162
GluI GCNGC 1 cut(s) 1399
HaeIII GGCC 1 cut(s) 843
HapII CCGG 2 cut(s) 197, 844
HgaI GACGC 2 cut(s) 273, 354
HhaI GCGC 1 cut(s) 163
Hin1I GRCGYC 1 cut(s) 365
Hin1II CATG 4 cut(s) 788, 807, 1094, 1438
Hin6I GCGC 1 cut(s) 161
HinP1I GCGC 1 cut(s) 161
HincII GTYRAC 1 cut(s) 9
HindII GTYRAC 1 cut(s) 9
HindIII AAGCTT 2 cut(s) 573, 584
HpaII CCGG 2 cut(s) 197, 844
HphI GGTGA 3 cut(s) 55, 201, 259
Hpy166II GTNNAC 3 cut(s) 9, 539, 1117
Hpy188III TCNNGA 5 cut(s) 77, 87, 139, 1105, 1355
Hpy8I GTNNAC 3 cut(s) 9, 539, 1117
Hpy99I CGWCG 2 cut(s) 13, 1348
HpyAV CCTTC 3 cut(s) 259, 601, 1251
HpyCH4III ACNGT 1 cut(s) 1348
HpyCH4IV ACGT 2 cut(s) 1007, 1153
HpyCH4V TGCA 5 cut(s) 297, 562, 986, 1157, 1401
HpyF10VI GCNNNNNNNGC 1 cut(s) 849
HpySE526I ACGT 2 cut(s) 1007, 1153
Hsp92I GRCGYC 1 cut(s) 365
Hsp92II CATG 4 cut(s) 788, 807, 1094, 1438
HspAI GCGC 1 cut(s) 161
KpnI GGTACC 1 cut(s) 1352
LmnI GCTCC 2 cut(s) 305, 1131
Lsp1109I GCAGC 1 cut(s) 1385
LweI GCATC 3 cut(s) 549, 571, 1385
MabI ACCWGGT 1 cut(s) 1170
MaeI CTAG 6 cut(s) 480, 566, 767, 773, 1193, 1235
MaeII ACGT 2 cut(s) 1007, 1153
MaeIII GTNAC 5 cut(s) 366, 610, 919, 1174, 1418
MflI RGATCY 3 cut(s) 49, 1357, 1427
MlyI GAGTC 3 cut(s) 14, 31, 124
MmeI TCCRAC 2 cut(s) 990, 1158
Mph1103I ATGCAT 1 cut(s) 564
MseI TTAA 7 cut(s) 570, 639, 752, 939, 1083, 1121, 1368
MspI CCGG 2 cut(s) 197, 844
MspR9I CCNGG 3 cut(s) 666, 1054, 1172
Mva1269I GAATGC 1 cut(s) 28
MvaI CCWGG 3 cut(s) 666, 1054, 1172
MvnI CGCG 1 cut(s) 284
MwoI GCNNNNNNNGC 1 cut(s) 849
NheI GCTAGC 1 cut(s) 1234
NlaIII CATG 4 cut(s) 788, 807, 1094, 1438
NlaIV GGNNCC 3 cut(s) 51, 1278, 1350
NmuCI GTSAC 3 cut(s) 366, 919, 1418
NsbI TGCGCA 1 cut(s) 162
NsiI ATGCAT 1 cut(s) 564
NspV TTCGAA 2 cut(s) 605, 867
PaeR7I CTCGAG 2 cut(s) 137, 1353
PctI GAATGC 1 cut(s) 28
PflMI CCANNNNNTGG 2 cut(s) 433, 740
PkrI GCNGC 1 cut(s) 1400
PleI GAGTC 3 cut(s) 13, 31, 124
PpsI GAGTC 3 cut(s) 13, 31, 124
Psp6I CCWGG 3 cut(s) 664, 1052, 1170
PspGI CCWGG 3 cut(s) 664, 1052, 1170
PspN4I GGNNCC 3 cut(s) 51, 1278, 1350
PspPI GGNCC 1 cut(s) 1276
PstI CTGCAG 1 cut(s) 1403
PstNI CAGNNNCTG 1 cut(s) 695
PsuI RGATCY 3 cut(s) 49, 1357, 1427
RsaI GTAC 2 cut(s) 1350, 1405
RsaNI GTAC 2 cut(s) 1349, 1404
SalI GTCGAC 1 cut(s) 7
SaqAI TTAA 7 cut(s) 570, 639, 752, 939, 1083, 1121, 1368
SatI GCNGC 1 cut(s) 1399
Sau96I GGNCC 1 cut(s) 1276
SchI GAGTC 3 cut(s) 14, 31, 124
ScrFI CCNGG 3 cut(s) 666, 1054, 1172
SexAI ACCWGGT 1 cut(s) 1170
SfaNI GCATC 3 cut(s) 549, 571, 1385
SfcI CTRYAG 1 cut(s) 1399
Sfr274I CTCGAG 2 cut(s) 137, 1353
SfuI TTCGAA 2 cut(s) 605, 867
SinI GGWCC 1 cut(s) 1276
SlaI CTCGAG 2 cut(s) 137, 1353
SmlI CTYRAG 4 cut(s) 75, 137, 1041, 1353
SmoI CTYRAG 4 cut(s) 75, 137, 1041, 1353
SsiI CCGC 3 cut(s) 200, 282, 1376
SspMI CTAG 6 cut(s) 480, 566, 767, 773, 1193, 1235
StyD4I CCNGG 3 cut(s) 664, 1052, 1170
StyI CCWWGG 1 cut(s) 69
TaaI ACNGT 1 cut(s) 1348
TaiI ACGT 2 cut(s) 1010, 1156
TatI WGTACW 1 cut(s) 1403
Tru1I TTAA 7 cut(s) 570, 639, 752, 939, 1083, 1121, 1368
Tru9I TTAA 7 cut(s) 570, 639, 752, 939, 1083, 1121, 1368
TscAI CASTG 1 cut(s) 1429
TseFI GTSAC 3 cut(s) 366, 919, 1418
TseI GCWGC 1 cut(s) 1398
Tsp45I GTSAC 3 cut(s) 366, 919, 1418
TspDTI ATGAA 9 cut(s) 341, 462, 489, 509, 714, 789, 983, 1080, 1306
TspGWI ACGGA 2 cut(s) 229, 391
TspRI CASTG 1 cut(s) 1429
Van91I CCANNNNNTGG 2 cut(s) 433, 740
VpaK11BI GGWCC 1 cut(s) 1276
XapI RAATTY 7 cut(s) 13, 100, 256, 430, 458, 545, 1160
XcmI CCANNNNNNNNNTGG 1 cut(s) 430
XhoI CTCGAG 2 cut(s) 137, 1353
XmiI GTMKAC 3 cut(s) 8, 538, 1116
XspI CTAG 6 cut(s) 480, 566, 767, 773, 1193, 1235
Zsp2I ATGCAT 1 cut(s) 564
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.