FvH4_6g47160

Targeting protein for Xklp2 (TPX2)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0004726
Physical Location & Seq
Reverse (-)
35815890 .. 35819500
3611 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g47160.t2

Sequence Viewer

Length: 1479 bp
ATGGAATCCGCCGGCAAGAGCGCCGGAGCCGTGACTCCGATCAAAGACCACCACGGCTCATCCCGATCCAAGAAGCAGGAGAATCCCCGCCACTCGGAGAATCTAAACCCTAACGTCTCCCCCGGCCTGAGATCCGCCAAGTCGCAGAAATCGGCGCCGAAGAATCCGAATCCGAACCCGAACCCGAAACCGGTTGTGTTTCAGTCGCCGAAAAAGAAGATCCGGGAGAGGAAGTTCGTGGTGGCGAAGAAGAGAGTTAAGAAGGAAGATCCAGATCCGAATTCGGACCCGAATCCGAACGCCGAGTGCGGAAAGTGCAAAGAGAGAGGGAAGAAGTGTATGTGCTTGGCTTACGAGACTCTCAGGGCTTCTCAGGAGGAGTTCTTCAAGACCCGACCCGAACCCGAACACGAAAGCGGGTCCAAGGAGGCCGATCGGGAAATCGAGGAGGCGTTGCTGATTCAGGATCGGGATTTTGACGATCGCGGCCCGGGCGAGGCCGACCCGACTGAGGAAACGGGTCCGGGTTGCTCGACGGTTAAGAGGAGGAGGGCGAGGCTGTTGGAGGAGGCGAGGCAGAGCGTGCCGGAGCGTGGGAAGGTGATGCATTTGGTTCAGGCCTTCGAGAAGCTGCTCACGATTCCCAAGGGAGAAGAAGAAGGAGGAGAAGAAGAGAGTGATGATAAGAAGAAGAAGGCGACGAAATGGGCCTTGCCTGGGCTTCAACCACCGCAGGCACACGAGTCGTCATCGTCGTTTTGCCCGTCGGATTTGTTTCTGACGTCTGAGAATCTCGGGCTGGATCGCCGGCCGTCGGTTTCGTCGTCTTGGGATGGAAGCCTGGGAAGCGTTTCGAACAATGGGGGGAGGAGAAGCCGGAGAAATAGCTCGGAGTCGTCTGGCACGATTGGAGGAAGCAGATGGAAGAAGAAGCAGCAGCAGAGAGCCACTAGCCTCAAACCATTCAAGCTCAGGACAGAGGAAAGGGGGAGAATGAAGGGAGAAGAGTTCATGAAGAAGGTGCAGGAGATGATGATACAGGAGGAGAGGCAGCGGATACCAATTGCTCAGGGACTCCCATGGACAACAGATGAACCAGAGTGCTTAATTAAACCTCCTGTCAAAGAGAGCACAAGACCAACAGACCCGAAGCTCCACACTGACATGCGGGCAGTAGAGCGTGCTGAGTTTGATCATCAGGTGGCAGAGAAGCTGAGCCTGTTTGAGCAATACAAGATGGAAAGAGAGAGACTGCAGAAGTTGGCAGAAGAGGAAGAAGTAAGAAGGCTGAGAAAGGAGCTTGTTCCAAAAGCACAGCCAATGCCCTACTTTGACAGGCCTTTCATTCCCAGAAGGTCAATGAAGCATCCAACCATACCCAAAGACCCAAAGTTCCATATCCCTCAACATAAGAAGATCAAGTGTTGCATGTCATGGAATGATGATATGAGCTCCTACTCTTATCAAAGTGAAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPX2

Protein Analysis

493

Amino Acids

56.1

Weight (kDa)

9.31

Isoelectric Point (pI)

68.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPX2 PF06886 384 - 454 3.2e-23 Targeting protein for Xklp2 (TPX2) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 785
AccB1I GGYRCC 1 cut(s) 154
AccII CGCG 1 cut(s) 486
AciI CCGC 9 cut(s) 9, 88, 135, 309, 417, 486, 731, 1054, 1168
AclWI GGATC 7 cut(s) 60, 126, 214, 263, 269, 474, 810
AcoI YGGCCR 1 cut(s) 809
AcsI RAATTY 1 cut(s) 280
AcyI GRCGYC 2 cut(s) 155, 782
AfiI CCNNNNNNNGG 7 cut(s) 94, 190, 496, 511, 593, 717, 814
AgeI ACCGGT 1 cut(s) 190
AgsI TTSAA 3 cut(s) 388, 725, 967
AjnI CCWGG 2 cut(s) 715, 840
AloI GAACNNNNNNTCC 2 cut(s) 218, 250
AluBI AGCT 8 cut(s) 631, 888, 970, 1153, 1213, 1300, 1452, 1475
AluI AGCT 8 cut(s) 631, 888, 970, 1153, 1213, 1300, 1452, 1475
Alw21I GWGCWC 2 cut(s) 1133, 1454
Alw26I GTCTC 3 cut(s) 121, 350, 1243
AlwI GGATC 7 cut(s) 60, 126, 214, 263, 269, 474, 810
Ama87I CYCGRG 2 cut(s) 490, 794
AoxI GGCC 8 cut(s) 124, 429, 487, 498, 618, 708, 809, 1337
ApeKI GCWGC 4 cut(s) 631, 934, 937, 1051
ApoI RAATTY 1 cut(s) 280
AsiGI ACCGGT 1 cut(s) 190
Asp700I GAANNNNTTC 1 cut(s) 850
AspLEI GCGC 2 cut(s) 23, 157
AspS9I GGNCC 5 cut(s) 286, 420, 488, 521, 708
AsuC2I CCSGG 5 cut(s) 123, 224, 491, 492, 525
AsuHPI GGTGA 1 cut(s) 613
AsuII TTCGAA 1 cut(s) 854
AvaI CYCGRG 2 cut(s) 490, 794
AvaII GGWCC 3 cut(s) 286, 420, 521
BanI GGYRCC 1 cut(s) 154
BanII GRGCYC 1 cut(s) 1454
BauI CACGAG 1 cut(s) 740
Bbv12I GWGCWC 2 cut(s) 1133, 1454
BbvI GCAGC 4 cut(s) 618, 946, 949, 1063
BccI CCATC 3 cut(s) 827, 915, 1231
BceAI ACGGC 3 cut(s) 14, 70, 796
BcgI CGANNNNNNTGC 2 cut(s) 726, 760
BciT130I CCWGG 2 cut(s) 717, 842
BciVI GTATCC 1 cut(s) 1050
BclI TGATCA 1 cut(s) 1192
BcnI CCSGG 5 cut(s) 123, 224, 491, 492, 525
BcoDI GTCTC 3 cut(s) 121, 350, 1243
BfaI CTAG 1 cut(s) 951
BfmI CTRYAG 1 cut(s) 1253
BfoI RGCGCY 2 cut(s) 24, 158
BfuI GTATCC 1 cut(s) 1050
BisI GCNGC 5 cut(s) 487, 632, 935, 938, 1052
BlpI GCTNAGC 1 cut(s) 1214
BlsI GCNGC 5 cut(s) 488, 633, 936, 939, 1053
Bme1390I CCNGG 7 cut(s) 123, 224, 491, 492, 525, 717, 842
Bme18I GGWCC 3 cut(s) 286, 420, 521
BmeT110I CYCGRG 2 cut(s) 490, 794
BmgT120I GGNCC 5 cut(s) 286, 420, 488, 521, 708
BmiI GGNNCC 5 cut(s) 28, 156, 288, 421, 522
BmrFI CCNGG 7 cut(s) 123, 224, 491, 492, 525, 717, 842
BmsI GCATC 2 cut(s) 594, 1375
Bpu10I CCTNAGC 2 cut(s) 971, 1068
Bpu1102I GCTNAGC 1 cut(s) 1214
Bpu14I TTCGAA 1 cut(s) 854
BpuMI CCSGG 5 cut(s) 123, 224, 491, 492, 525
BsaBI GATNNNNATC 1 cut(s) 273
BsaHI GRCGYC 2 cut(s) 155, 782
BsaJI CCNNGG 8 cut(s) 52, 121, 423, 490, 645, 716, 841, 1079
BsaWI WCCGGW 1 cut(s) 190
BsaXI ACNNNNNCTCC 2 cut(s) 218, 248
Bsc4I CCNNNNNNNGG 7 cut(s) 94, 190, 496, 511, 593, 717, 814
Bse118I RCCGGY 3 cut(s) 11, 190, 807
Bse8I GATNNNNATC 1 cut(s) 273
BseBI CCWGG 2 cut(s) 717, 842
BseDI CCNNGG 8 cut(s) 52, 121, 423, 490, 645, 716, 841, 1079
BseGI GGATG 3 cut(s) 59, 838, 1366
BseJI GATNNNNATC 1 cut(s) 273
BseLI CCNNNNNNNGG 7 cut(s) 94, 190, 496, 511, 593, 717, 814
BseRI GAGGAG 8 cut(s) 392, 461, 559, 562, 581, 678, 883, 1058
BseX3I CGGCCG 1 cut(s) 809
BseXI GCAGC 4 cut(s) 618, 946, 949, 1063
BsgI GTGCAG 1 cut(s) 1043
Bsh1236I CGCG 1 cut(s) 486
Bsh1285I CGRYCG 3 cut(s) 436, 484, 812
BshFI GGCC 8 cut(s) 126, 431, 489, 500, 620, 710, 811, 1339
BshNI GGYRCC 1 cut(s) 154
BshTI ACCGGT 1 cut(s) 190
BsiEI CGRYCG 3 cut(s) 436, 484, 812
BsiHKAI GWGCWC 2 cut(s) 1133, 1454
BsiHKCI CYCGRG 2 cut(s) 490, 794
BslFI GGGAC 1 cut(s) 1086
BslI CCNNNNNNNGG 7 cut(s) 94, 190, 496, 511, 593, 717, 814
BsmAI GTCTC 3 cut(s) 121, 350, 1243
BsmBI CGTCTC 1 cut(s) 121
BsmFI GGGAC 1 cut(s) 1086
BsnI GGCC 8 cut(s) 126, 431, 489, 500, 620, 710, 811, 1339
BsoBI CYCGRG 2 cut(s) 490, 794
Bsp119I TTCGAA 1 cut(s) 854
Bsp1286I GDGCHC 2 cut(s) 1133, 1454
Bsp1720I GCTNAGC 1 cut(s) 1214
Bsp19I CCATGG 1 cut(s) 1079
BspACI CCGC 9 cut(s) 9, 88, 135, 309, 417, 486, 731, 1054, 1168
BspANI GGCC 8 cut(s) 126, 431, 489, 500, 620, 710, 811, 1339
BspFNI CGCG 1 cut(s) 486
BspHI TCATGA 1 cut(s) 1011
BspLI GGNNCC 5 cut(s) 28, 156, 288, 421, 522
BspMAI CTGCAG 1 cut(s) 1257
BspPI GGATC 7 cut(s) 60, 126, 214, 263, 269, 474, 810
BspT104I TTCGAA 1 cut(s) 854
BspT107I GGYRCC 1 cut(s) 154
BsrFI RCCGGY 3 cut(s) 11, 190, 807
BssAI RCCGGY 3 cut(s) 11, 190, 807
BssECI CCNNGG 8 cut(s) 52, 121, 423, 490, 645, 716, 841, 1079
BssNI GRCGYC 2 cut(s) 155, 782
BssSI CACGAG 1 cut(s) 740
BssT1I CCWWGG 3 cut(s) 423, 645, 1079
Bst2BI CACGAG 1 cut(s) 740
Bst2UI CCWGG 2 cut(s) 717, 842
Bst4CI ACNGT 1 cut(s) 538
Bst6I CTCTTC 4 cut(s) 245, 666, 999, 1263
BstACI GRCGYC 2 cut(s) 155, 782
BstAPI GCANNNNNTGC 1 cut(s) 583
BstBI TTCGAA 1 cut(s) 854
BstC8I GCNNGC 6 cut(s) 13, 584, 735, 809, 1170, 1182
BstDSI CCRYGG 2 cut(s) 52, 1079
BstF5I GGATG 3 cut(s) 59, 838, 1366
BstFNI CGCG 1 cut(s) 486
BstH2I RGCGCY 2 cut(s) 24, 158
BstHHI GCGC 2 cut(s) 23, 157
BstMAI GTCTC 3 cut(s) 121, 350, 1243
BstMCI CGRYCG 3 cut(s) 436, 484, 812
BstMWI GCNNNNNNNGC 4 cut(s) 315, 492, 583, 846
BstNI CCWGG 2 cut(s) 717, 842
BstNSI RCATGY 2 cut(s) 1168, 1432
BstSCI CCNGG 7 cut(s) 121, 222, 489, 490, 523, 715, 840
BstSFI CTRYAG 1 cut(s) 1253
BstUI CGCG 1 cut(s) 486
BstV1I GCAGC 4 cut(s) 618, 946, 949, 1063
BstX2I RGATCY 4 cut(s) 131, 219, 268, 274
BstYI RGATCY 4 cut(s) 131, 219, 268, 274
BstZI CGGCCG 1 cut(s) 809
BsuI GTATCC 1 cut(s) 1050
BsuRI GGCC 8 cut(s) 126, 431, 489, 500, 620, 710, 811, 1339
BtgI CCRYGG 2 cut(s) 52, 1079
BtsCI GGATG 3 cut(s) 59, 838, 1366
BtsIMutI CAGTG 1 cut(s) 1158
Cac8I GCNNGC 6 cut(s) 13, 584, 735, 809, 1170, 1182
CciI TCATGA 1 cut(s) 1011
CfoI GCGC 2 cut(s) 23, 157
Cfr10I RCCGGY 3 cut(s) 11, 190, 807
Cfr13I GGNCC 5 cut(s) 286, 420, 488, 521, 708
Cfr9I CCCGGG 1 cut(s) 490
CspAI ACCGGT 1 cut(s) 190
CviAII CATG 5 cut(s) 1012, 1080, 1165, 1429, 1434
DinI GGCGCC 1 cut(s) 156
EaeI YGGCCR 1 cut(s) 809
EagI CGGCCG 1 cut(s) 809
Eam1104I CTCTTC 4 cut(s) 245, 666, 999, 1263
EarI CTCTTC 4 cut(s) 245, 666, 999, 1263
EciI GGCGGA 1 cut(s) 124
Ecl136II GAGCTC 1 cut(s) 1452
EclXI CGGCCG 1 cut(s) 809
Eco130I CCWWGG 3 cut(s) 423, 645, 1079
Eco147I AGGCCT 2 cut(s) 620, 1339
Eco24I GRGCYC 1 cut(s) 1454
Eco47I GGWCC 3 cut(s) 286, 420, 521
Eco52I CGGCCG 1 cut(s) 809
Eco53kI GAGCTC 1 cut(s) 1452
Eco88I CYCGRG 2 cut(s) 490, 794
EcoICRI GAGCTC 1 cut(s) 1452
EcoRI GAATTC 1 cut(s) 280
EcoRII CCWGG 2 cut(s) 715, 840
EcoT14I CCWWGG 3 cut(s) 423, 645, 1079
EcoT22I ATGCAT 1 cut(s) 609
EcoT38I GRGCYC 1 cut(s) 1454
EgeI GGCGCC 1 cut(s) 156
EheI GGCGCC 1 cut(s) 156
ErhI CCWWGG 3 cut(s) 423, 645, 1079
Esp3I CGTCTC 1 cut(s) 121
FaeI CATG 5 cut(s) 1015, 1083, 1168, 1432, 1437
FalI AAGNNNNNCTT 1 cut(s) 1459
FaqI GGGAC 1 cut(s) 1086
FatI CATG 5 cut(s) 1011, 1079, 1164, 1428, 1433
FauI CCCGC 3 cut(s) 95, 410, 1161
FbaI TGATCA 1 cut(s) 1192
Fnu4HI GCNGC 5 cut(s) 487, 632, 935, 938, 1052
FokI GGATG 3 cut(s) 46, 845, 1353
FriOI GRGCYC 1 cut(s) 1454
Fsp4HI GCNGC 5 cut(s) 487, 632, 935, 938, 1052
FspBI CTAG 1 cut(s) 951
GlaI GCGC 2 cut(s) 22, 156
GluI GCNGC 5 cut(s) 487, 632, 935, 938, 1052
HaeII RGCGCY 2 cut(s) 24, 158
HaeIII GGCC 8 cut(s) 126, 431, 489, 500, 620, 710, 811, 1339
HhaI GCGC 2 cut(s) 23, 157
Hin1I GRCGYC 2 cut(s) 155, 782
Hin1II CATG 5 cut(s) 1015, 1083, 1168, 1432, 1437
Hin6I GCGC 2 cut(s) 21, 155
HinP1I GCGC 2 cut(s) 21, 155
HindIII AAGCTT 1 cut(s) 1473
HphI GGTGA 1 cut(s) 613
Hpy99I CGWCG 6 cut(s) 538, 703, 757, 769, 817, 826
HpyAV CCTTC 9 cut(s) 256, 592, 631, 653, 688, 991, 1012, 1278, 1347
HpyCH4III ACNGT 1 cut(s) 538
HpyCH4IV ACGT 2 cut(s) 114, 782
HpyCH4V TGCA 5 cut(s) 318, 607, 1024, 1255, 1428
HpyF10VI GCNNNNNNNGC 4 cut(s) 315, 492, 583, 846
HpySE526I ACGT 2 cut(s) 114, 782
Hsp92I GRCGYC 2 cut(s) 155, 782
Hsp92II CATG 5 cut(s) 1015, 1083, 1168, 1432, 1437
HspAI GCGC 2 cut(s) 21, 155
KasI GGCGCC 1 cut(s) 154
KroI GCCGGC 2 cut(s) 11, 807
KroNI GCCGGC 2 cut(s) 13, 809
Ksp22I TGATCA 1 cut(s) 1192
LmnI GCTCC 5 cut(s) 26, 589, 1158, 1297, 1457
Lsp1109I GCAGC 4 cut(s) 618, 946, 949, 1063
LweI GCATC 2 cut(s) 594, 1375
MaeI CTAG 1 cut(s) 951
MaeII ACGT 2 cut(s) 114, 782
MaeIII GTNAC 1 cut(s) 31
MfeI CAATTG 1 cut(s) 1062
MflI RGATCY 4 cut(s) 131, 219, 268, 274
MhlI GDGCHC 2 cut(s) 1133, 1454
MluCI AATT 3 cut(s) 280, 1062, 1107
Mly113I GGCGCC 1 cut(s) 155
MlyI GAGTC 5 cut(s) 28, 352, 752, 902, 1068
MmeI TCCRAC 3 cut(s) 543, 747, 1394
Mph1103I ATGCAT 1 cut(s) 609
MroNI GCCGGC 2 cut(s) 11, 807
MroXI GAANNNNTTC 1 cut(s) 850
MseI TTAA 4 cut(s) 258, 540, 1106, 1110
MslI CAYNNNNRTG 1 cut(s) 1163
MspA1I CMGCKG 1 cut(s) 1054
MspR9I CCNGG 7 cut(s) 123, 224, 491, 492, 525, 717, 842
MunI CAATTG 1 cut(s) 1062
MvaI CCWGG 2 cut(s) 717, 842
MvnI CGCG 1 cut(s) 486
MwoI GCNNNNNNNGC 4 cut(s) 315, 492, 583, 846
NaeI GCCGGC 2 cut(s) 13, 809
NarI GGCGCC 1 cut(s) 155
NciI CCSGG 5 cut(s) 123, 224, 491, 492, 525
NcoI CCATGG 1 cut(s) 1079
NgoMIV GCCGGC 2 cut(s) 11, 807
NlaIII CATG 5 cut(s) 1015, 1083, 1168, 1432, 1437
NlaIV GGNNCC 5 cut(s) 28, 156, 288, 421, 522
NmeAIII GCCGAG 1 cut(s) 328
NmuCI GTSAC 1 cut(s) 31
NsiI ATGCAT 1 cut(s) 609
NspI RCATGY 2 cut(s) 1168, 1432
NspV TTCGAA 1 cut(s) 854
PacI TTAATTAA 1 cut(s) 1110
PagI TCATGA 1 cut(s) 1011
PceI AGGCCT 2 cut(s) 620, 1339
PcsI WCGNNNNNNNCGW 4 cut(s) 752, 761, 821, 902
PdiI GCCGGC 2 cut(s) 13, 809
PdmI GAANNNNTTC 1 cut(s) 850
PfeI GAWTC 9 cut(s) 5, 82, 100, 163, 169, 292, 460, 640, 790
PfoI TCCNGGA 1 cut(s) 222
PinAI ACCGGT 1 cut(s) 190
PkrI GCNGC 5 cut(s) 488, 633, 936, 939, 1053
Ple19I CGATCG 2 cut(s) 436, 484
PleI GAGTC 5 cut(s) 28, 352, 751, 901, 1068
PluTI GGCGCC 1 cut(s) 158
PpsI GAGTC 5 cut(s) 28, 352, 751, 901, 1068
Psp124BI GAGCTC 1 cut(s) 1454
Psp6I CCWGG 2 cut(s) 715, 840
PspGI CCWGG 2 cut(s) 715, 840
PspN4I GGNNCC 5 cut(s) 28, 156, 288, 421, 522
PspPI GGNCC 5 cut(s) 286, 420, 488, 521, 708
PstI CTGCAG 1 cut(s) 1257
PsuI RGATCY 4 cut(s) 131, 219, 268, 274
PvuI CGATCG 2 cut(s) 436, 484
RseI CAYNNNNRTG 1 cut(s) 1163
SacI GAGCTC 1 cut(s) 1454
SaqAI TTAA 4 cut(s) 258, 540, 1106, 1110
SatI GCNGC 5 cut(s) 487, 632, 935, 938, 1052
Sau96I GGNCC 5 cut(s) 286, 420, 488, 521, 708
SchI GAGTC 5 cut(s) 28, 352, 752, 902, 1068
ScrFI CCNGG 7 cut(s) 123, 224, 491, 492, 525, 717, 842
SduI GDGCHC 2 cut(s) 1133, 1454
SfaNI GCATC 2 cut(s) 594, 1375
SfcI CTRYAG 1 cut(s) 1253
SfoI GGCGCC 1 cut(s) 156
SfuI TTCGAA 1 cut(s) 854
SinI GGWCC 3 cut(s) 286, 420, 521
SmaI CCCGGG 1 cut(s) 492
SmiMI CAYNNNNRTG 1 cut(s) 1163
SrfI GCCCGGGC 1 cut(s) 492
Sse9I AATT 3 cut(s) 280, 1062, 1107
SseBI AGGCCT 2 cut(s) 620, 1339
SsiI CCGC 9 cut(s) 9, 88, 135, 309, 417, 486, 731, 1054, 1168
SspDI GGCGCC 1 cut(s) 154
SspMI CTAG 1 cut(s) 951
SstI GAGCTC 1 cut(s) 1454
StuI AGGCCT 2 cut(s) 620, 1339
StyD4I CCNGG 7 cut(s) 121, 222, 489, 490, 523, 715, 840
StyI CCWWGG 3 cut(s) 423, 645, 1079
TaaI ACNGT 1 cut(s) 538
TaiI ACGT 2 cut(s) 117, 785
TaqI TCGA 4 cut(s) 444, 533, 624, 854
TasI AATT 3 cut(s) 280, 1062, 1107
TauI GCSGC 1 cut(s) 489
TfiI GAWTC 9 cut(s) 5, 82, 100, 163, 169, 292, 460, 640, 790
Tru1I TTAA 4 cut(s) 258, 540, 1106, 1110
Tru9I TTAA 4 cut(s) 258, 540, 1106, 1110
TscAI CASTG 1 cut(s) 1165
TseFI GTSAC 1 cut(s) 31
TseI GCWGC 4 cut(s) 631, 934, 937, 1051
Tsp45I GTSAC 1 cut(s) 31
TspDTI ATGAA 6 cut(s) 1000, 1010, 1028, 1107, 1333, 1376
TspMI CCCGGG 1 cut(s) 490
TspRI CASTG 1 cut(s) 1165
VpaK11BI GGWCC 3 cut(s) 286, 420, 521
XapI RAATTY 1 cut(s) 280
XceI RCATGY 2 cut(s) 1168, 1432
XmaI CCCGGG 1 cut(s) 490
XmnI GAANNNNTTC 1 cut(s) 850
XspI CTAG 1 cut(s) 951
ZraI GACGTC 1 cut(s) 783
Zsp2I ATGCAT 1 cut(s) 609
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.