AT5G15510

TPX2 (targeting protein for Xklp2) protein family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0004726
Physical Location & Seq
Forward (+)
5033933 .. 5036824
2892 bp
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UTR
Exon/CDS
Intron
AT5G15510.2

Sequence Viewer

Length: 1494 bp
ATGGAGTCGTTGACATTGAAGAATGGGAAAGCAGGGACTCCGGTGAAGGATCGGAGCAAGTTTCAGTCCAATCAAAAGATTTCCAGATCATCGGAGAATGCAAACCCTAACATTTCACAAGCGAGTCCTTCTAAATCACCGCTCATGAAATCTGCGAAGAAATCAGCGCAGAAGAATCCGAGTCCGAGCCCTAAACCTACCCAGGCGGTTTTCTCTCCCCGGAATCGGATCAGAGAGAGGAAGTTTGTTGTGGTGGCGAAGAAGAATTCGAGGAAAGGGAAGAAGGAGCCAGCGATTACAGAATCCAAGGCAGCTGAGATAGATTGTAAATGCGGAGAGAGGAAGAAGGGGAACATGAAGTGTGTTTGTGTTGCATATGAAACTCTGCGTGCTTCGCAAGAGGAGTTCTTCAAAAATCGAATTGAATCAGAGGAGGATAAGGAAGCTTTGGAGGAATGCTGTAATCTCGGAAATGGCGATGAACTAGAAGGAAAAGAATCTGGAGAACCGGAGAAAACAGGCGTTTCCACAATGAAGAGATCAAGAGCTAAGGTGGTGGAAGAAGCACGGCAAAGTGTGCCTGTATCTGGTAAAGTGATGAATCTTGTAGAAGCATTTGAGAAACTCACTTGCTTTAGTAATTCAAAGACTGCCAACAAGATAGAAGAGAATCAAACCGAAGAGGATACGAAGAAACCTGTGAAATTGGAATTCTTGGAGGGTGAGAAAGAGAAAAACCCATGGAGTTCTTCGTTTTGTCCATCTGAAATGGTCTTAACAGCTAAGAATCTGGGTTTAGACCCCAATGCTTCGATTTCTTCGTCCTGGGACAGCACCCGTGGCAGTGTTTTGAGTGGAAGTTCAAATGCTGGCAGGAGAAGTAGAAGAAATAGCATGGAGTCATCGACTACAATGGGAAGTAGAAGATCAAAGAAGAAGCAGGTTAAGGTCACTTCATTGAAGCCGTTTAAGCTTAGAACTGAGCAAAGAGGTAAAGTGAAAGAGGAAGAGTTAGCAAAAAAGATACACGAAATAACAATGGAGGAAGAAAAGATGAGAATCCCCATTGCTCAAGGTCTTCCATGGACAACCGATGAACCTGAGTGCCTAGTGAAGCCTCATTGGAAAGACATTACAAGACCAGTCGACTTGACGCTCCACTCAGATGTCCGGGCAGTTGAACGCGCAGAATTTGATTACCAGGTTGCCGAGAAGATGAGCTTCATTGAGCAATACAAAATGGAAAGAGAAAGACAACAGAAGTTGGCAGAGGAAGAAGAGATAAGAAGGCTGAGGAAAGAATTGGTCCCAAAAGCACAACCAATGCCATACTTTGATCGACCATTCATTCCAAGAAGGTCGAGCAAACATCCAACTGCACCACGAGATCCCAAGTTTCACATACCGCAACACAAGAAGATCAGATGCTGCAGTTCATCTTCTTGGAGTGAAACTGGCTCCTGCATGAGCGATTTCCAATATCAGTTTCTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPX2

Protein Analysis

497

Amino Acids

56.48

Weight (kDa)

9.44

Isoelectric Point (pI)

66.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPX2 PF06886 385 - 453 3.2e-24 Targeting protein for Xklp2 (TPX2) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 931
AccBSI CCGCTC 1 cut(s) 142
AccI GTMKAC 1 cut(s) 1146
AccII CGCG 1 cut(s) 1185
AciI CCGC 4 cut(s) 140, 206, 333, 1406
AclWI GGATC 3 cut(s) 57, 236, 1382
AcsI RAATTY 3 cut(s) 265, 710, 1190
AfiI CCNNNNNNNGG 2 cut(s) 225, 587
AgsI TTSAA 7 cut(s) 19, 412, 425, 645, 864, 961, 1181
AjnI CCWGG 3 cut(s) 201, 824, 1200
AjuI GAANNNNNNNTTGG 1 cut(s) 1470
AluBI AGCT 6 cut(s) 314, 446, 548, 782, 973, 1221
AluI AGCT 6 cut(s) 314, 446, 548, 782, 973, 1221
AlwI GGATC 3 cut(s) 57, 236, 1382
AlwNI CAGNNNCTG 1 cut(s) 1428
ApeKI GCWGC 2 cut(s) 311, 1428
ApoI RAATTY 3 cut(s) 265, 710, 1190
AspLEI GCGC 2 cut(s) 169, 1187
AspS9I GGNCC 1 cut(s) 1306
AsuC2I CCSGG 2 cut(s) 220, 1172
AsuHPI GGTGA 3 cut(s) 55, 129, 734
AvaII GGWCC 1 cut(s) 1306
BanII GRGCYC 1 cut(s) 191
BauI CACGAG 1 cut(s) 1383
BbsI GAAGAC 1 cut(s) 1070
BbvCI CCTCAGC 1 cut(s) 1292
BbvI GCAGC 2 cut(s) 323, 1415
BccI CCATC 1 cut(s) 769
BceAI ACGGC 2 cut(s) 584, 949
BciT130I CCWGG 3 cut(s) 203, 826, 1202
BciVI GTATCC 1 cut(s) 679
BcnI CCSGG 2 cut(s) 220, 1172
BfaI CTAG 3 cut(s) 485, 1109, 1492
BfmI CTRYAG 1 cut(s) 1429
BfuAI ACCTGC 1 cut(s) 931
BfuI GTATCC 1 cut(s) 679
BisI GCNGC 2 cut(s) 312, 1429
BlsI GCNGC 2 cut(s) 313, 1430
Bme1390I CCNGG 5 cut(s) 203, 220, 826, 1172, 1202
Bme18I GGWCC 1 cut(s) 1306
BmgT120I GGNCC 1 cut(s) 1306
BmiI GGNNCC 3 cut(s) 288, 1308, 1459
BmrFI CCNGG 5 cut(s) 203, 220, 826, 1172, 1202
BmsI GCATC 1 cut(s) 1415
BpiI GAAGAC 1 cut(s) 1070
BpmI CTGGAG 1 cut(s) 522
Bpu10I CCTNAGC 2 cut(s) 549, 1292
BpuEI CTTGAG 1 cut(s) 1056
BpuMI CCSGG 2 cut(s) 220, 1172
BsaBI GATNNNNATC 1 cut(s) 1058
BsaJI CCNNGG 7 cut(s) 201, 218, 306, 740, 825, 838, 1082
BsaWI WCCGGW 2 cut(s) 40, 508
Bsc4I CCNNNNNNNGG 2 cut(s) 225, 587
Bse1I ACTGG 2 cut(s) 1142, 1459
Bse3DI GCAATG 1 cut(s) 1065
Bse8I GATNNNNATC 1 cut(s) 1058
BseBI CCWGG 3 cut(s) 203, 826, 1202
BseDI CCNNGG 7 cut(s) 201, 218, 306, 740, 825, 838, 1082
BseGI GGATG 1 cut(s) 1369
BseJI GATNNNNATC 1 cut(s) 1058
BseLI CCNNNNNNNGG 2 cut(s) 225, 587
BseMI GCAATG 1 cut(s) 1065
BseMII CTCAG 5 cut(s) 306, 972, 1092, 1176, 1283
BseNI ACTGG 2 cut(s) 1142, 1459
BseRI GAGGAG 2 cut(s) 416, 446
BseXI GCAGC 2 cut(s) 323, 1415
BsgI GTGCAG 1 cut(s) 1362
Bsh1236I CGCG 1 cut(s) 1185
BsiSI CCGG 4 cut(s) 41, 220, 509, 1171
BslFI GGGAC 3 cut(s) 49, 842, 1292
BslI CCNNNNNNNGG 2 cut(s) 225, 587
BsmFI GGGAC 3 cut(s) 49, 842, 1292
BsmI GAATGC 2 cut(s) 103, 461
Bsp1286I GDGCHC 1 cut(s) 191
Bsp143I GATC 8 cut(s) 49, 86, 228, 539, 926, 1336, 1387, 1419
Bsp19I CCATGG 2 cut(s) 740, 1082
BspACI CCGC 4 cut(s) 140, 206, 333, 1406
BspCNI CTCAG 5 cut(s) 307, 973, 1093, 1175, 1284
BspFNI CGCG 1 cut(s) 1185
BspHI TCATGA 1 cut(s) 144
BspLI GGNNCC 3 cut(s) 288, 1308, 1459
BspMAI CTGCAG 1 cut(s) 1433
BspMI ACCTGC 1 cut(s) 931
BspPI GGATC 3 cut(s) 57, 236, 1382
BsrBI CCGCTC 1 cut(s) 142
BsrDI GCAATG 1 cut(s) 1065
BsrI ACTGG 2 cut(s) 1142, 1459
BssECI CCNNGG 7 cut(s) 201, 218, 306, 740, 825, 838, 1082
BssMI GATC 8 cut(s) 49, 86, 228, 539, 926, 1336, 1387, 1419
BssSI CACGAG 1 cut(s) 1383
BssT1I CCWWGG 3 cut(s) 306, 740, 1082
Bst2BI CACGAG 1 cut(s) 1383
Bst2UI CCWGG 3 cut(s) 203, 826, 1202
Bst6I CTCTTC 5 cut(s) 530, 660, 675, 1002, 1272
BstAPI GCANNNNNTGC 1 cut(s) 577
BstC8I GCNNGC 3 cut(s) 291, 390, 871
BstDEI CTNAG 8 cut(s) 315, 549, 783, 974, 981, 1101, 1162, 1292
BstDSI CCRYGG 3 cut(s) 740, 838, 1082
BstF5I GGATG 1 cut(s) 1369
BstFNI CGCG 1 cut(s) 1185
BstHHI GCGC 2 cut(s) 169, 1187
BstKTI GATC 8 cut(s) 52, 89, 231, 542, 929, 1339, 1390, 1422
BstMBI GATC 8 cut(s) 49, 86, 228, 539, 926, 1336, 1387, 1419
BstMWI GCNNNNNNNGC 4 cut(s) 394, 577, 840, 970
BstNI CCWGG 3 cut(s) 203, 826, 1202
BstSCI CCNGG 5 cut(s) 201, 218, 824, 1170, 1200
BstSFI CTRYAG 1 cut(s) 1429
BstUI CGCG 1 cut(s) 1185
BstV1I GCAGC 2 cut(s) 323, 1415
BstV2I GAAGAC 1 cut(s) 1070
BstX2I RGATCY 1 cut(s) 1387
BstYI RGATCY 1 cut(s) 1387
BsuI GTATCC 1 cut(s) 679
BtgI CCRYGG 3 cut(s) 740, 838, 1082
BtgZI GCGATG 1 cut(s) 492
BtsCI GGATG 1 cut(s) 1369
BtsI GCAGTG 1 cut(s) 850
BtsIMutI CAGTG 1 cut(s) 850
BveI ACCTGC 1 cut(s) 931
Cac8I GCNNGC 3 cut(s) 291, 390, 871
CaiI CAGNNNCTG 1 cut(s) 1428
CciI TCATGA 1 cut(s) 144
CfoI GCGC 2 cut(s) 169, 1187
Cfr13I GGNCC 1 cut(s) 1306
CseI GACGC 1 cut(s) 1162
CsiI ACCWGGT 1 cut(s) 1200
CviAII CATG 6 cut(s) 145, 355, 741, 895, 1083, 1465
DdeI CTNAG 8 cut(s) 315, 549, 783, 974, 981, 1101, 1162, 1292
DpnI GATC 8 cut(s) 51, 88, 230, 541, 928, 1338, 1389, 1421
DpnII GATC 8 cut(s) 49, 86, 228, 539, 926, 1336, 1387, 1419
Eam1104I CTCTTC 5 cut(s) 530, 660, 675, 1002, 1272
EarI CTCTTC 5 cut(s) 530, 660, 675, 1002, 1272
Eco130I CCWWGG 3 cut(s) 306, 740, 1082
Eco24I GRGCYC 1 cut(s) 191
Eco47I GGWCC 1 cut(s) 1306
EcoRI GAATTC 2 cut(s) 265, 710
EcoRII CCWGG 3 cut(s) 201, 824, 1200
EcoT14I CCWWGG 3 cut(s) 306, 740, 1082
EcoT38I GRGCYC 1 cut(s) 191
ErhI CCWWGG 3 cut(s) 306, 740, 1082
FaeI CATG 6 cut(s) 148, 358, 744, 898, 1086, 1468
FalI AAGNNNNNCTT 2 cut(s) 1205, 1237
FaqI GGGAC 3 cut(s) 49, 842, 1292
FatI CATG 6 cut(s) 144, 354, 740, 894, 1082, 1464
FauNDI CATATG 1 cut(s) 376
FblI GTMKAC 1 cut(s) 1146
Fnu4HI GCNGC 2 cut(s) 312, 1429
FokI GGATG 1 cut(s) 1356
FriOI GRGCYC 1 cut(s) 191
Fsp4HI GCNGC 2 cut(s) 312, 1429
FspBI CTAG 3 cut(s) 485, 1109, 1492
GlaI GCGC 2 cut(s) 168, 1186
GluI GCNGC 2 cut(s) 312, 1429
GsuI CTGGAG 1 cut(s) 522
HapII CCGG 4 cut(s) 41, 220, 509, 1171
HgaI GACGC 1 cut(s) 1162
HhaI GCGC 2 cut(s) 169, 1187
Hin1II CATG 6 cut(s) 148, 358, 744, 898, 1086, 1468
Hin6I GCGC 2 cut(s) 167, 1185
HinP1I GCGC 2 cut(s) 167, 1185
HincII GTYRAC 2 cut(s) 12, 1147
HindII GTYRAC 2 cut(s) 12, 1147
HindIII AAGCTT 2 cut(s) 444, 971
HpaII CCGG 4 cut(s) 41, 220, 509, 1171
HphI GGTGA 3 cut(s) 55, 129, 734
Hpy166II GTNNAC 2 cut(s) 12, 1147
Hpy188III TCNNGA 4 cut(s) 84, 145, 501, 543
Hpy8I GTNNAC 2 cut(s) 12, 1147
HpyAV CCTTC 7 cut(s) 40, 138, 277, 340, 482, 1281, 1350
HpyCH4V TGCA 5 cut(s) 101, 374, 1379, 1431, 1464
HpyF10VI GCNNNNNNNGC 4 cut(s) 394, 577, 840, 970
HpyF3I CTNAG 8 cut(s) 315, 549, 783, 974, 981, 1101, 1162, 1292
Hsp92II CATG 6 cut(s) 148, 358, 744, 898, 1086, 1468
HspAI GCGC 2 cut(s) 167, 1185
Kzo9I GATC 8 cut(s) 49, 86, 228, 539, 926, 1336, 1387, 1419
LmnI GCTCC 4 cut(s) 54, 286, 1161, 1463
Lsp1109I GCAGC 2 cut(s) 323, 1415
LweI GCATC 1 cut(s) 1415
MabI ACCWGGT 1 cut(s) 1200
MaeI CTAG 3 cut(s) 485, 1109, 1492
MaeIII GTNAC 1 cut(s) 949
MalI GATC 8 cut(s) 51, 88, 230, 541, 928, 1338, 1389, 1421
MbiI CCGCTC 1 cut(s) 142
MboI GATC 8 cut(s) 49, 86, 228, 539, 926, 1336, 1387, 1419
MflI RGATCY 1 cut(s) 1387
MhlI GDGCHC 1 cut(s) 191
MluCI AATT 7 cut(s) 265, 420, 640, 704, 710, 1190, 1301
MlyI GAGTC 5 cut(s) 14, 31, 133, 190, 908
MmeI TCCRAC 1 cut(s) 1397
MseI TTAA 3 cut(s) 776, 945, 969
MslI CAYNNNNRTG 1 cut(s) 1164
MspA1I CMGCKG 1 cut(s) 314
MspI CCGG 4 cut(s) 41, 220, 509, 1171
MspR9I CCNGG 5 cut(s) 203, 220, 826, 1172, 1202
Mva1269I GAATGC 2 cut(s) 103, 461
MvaI CCWGG 3 cut(s) 203, 826, 1202
MvnI CGCG 1 cut(s) 1185
MwoI GCNNNNNNNGC 4 cut(s) 394, 577, 840, 970
NciI CCSGG 2 cut(s) 220, 1172
NcoI CCATGG 2 cut(s) 740, 1082
NdeI CATATG 1 cut(s) 376
NdeII GATC 8 cut(s) 49, 86, 228, 539, 926, 1336, 1387, 1419
NlaIII CATG 6 cut(s) 148, 358, 744, 898, 1086, 1468
NlaIV GGNNCC 3 cut(s) 288, 1308, 1459
NmeAIII GCCGAG 1 cut(s) 1234
NmuCI GTSAC 1 cut(s) 949
PagI TCATGA 1 cut(s) 144
PcsI WCGNNNNNNNCGW 2 cut(s) 474, 818
PctI GAATGC 2 cut(s) 103, 461
PfeI GAWTC 9 cut(s) 175, 223, 302, 425, 497, 601, 670, 787, 1059
PkrI GCNGC 2 cut(s) 313, 1430
PleI GAGTC 5 cut(s) 13, 31, 132, 189, 907
PpsI GAGTC 5 cut(s) 13, 31, 132, 189, 907
Psp6I CCWGG 3 cut(s) 201, 824, 1200
PspGI CCWGG 3 cut(s) 201, 824, 1200
PspN4I GGNNCC 3 cut(s) 288, 1308, 1459
PspPI GGNCC 1 cut(s) 1306
PstI CTGCAG 1 cut(s) 1433
PstNI CAGNNNCTG 1 cut(s) 1428
PsuI RGATCY 1 cut(s) 1387
PvuII CAGCTG 1 cut(s) 314
RseI CAYNNNNRTG 1 cut(s) 1164
SalI GTCGAC 1 cut(s) 1145
SaqAI TTAA 3 cut(s) 776, 945, 969
SatI GCNGC 2 cut(s) 312, 1429
Sau3AI GATC 8 cut(s) 49, 86, 228, 539, 926, 1336, 1387, 1419
Sau96I GGNCC 1 cut(s) 1306
SchI GAGTC 5 cut(s) 14, 31, 133, 190, 908
ScrFI CCNGG 5 cut(s) 203, 220, 826, 1172, 1202
SduI GDGCHC 1 cut(s) 191
SexAI ACCWGGT 1 cut(s) 1200
SfaNI GCATC 1 cut(s) 1415
SfcI CTRYAG 1 cut(s) 1429
SinI GGWCC 1 cut(s) 1306
SmiMI CAYNNNNRTG 1 cut(s) 1164
SmlI CTYRAG 1 cut(s) 1071
SmoI CTYRAG 1 cut(s) 1071
Sse9I AATT 7 cut(s) 265, 420, 640, 704, 710, 1190, 1301
SsiI CCGC 4 cut(s) 140, 206, 333, 1406
SspMI CTAG 3 cut(s) 485, 1109, 1492
StyD4I CCNGG 5 cut(s) 201, 218, 824, 1170, 1200
StyI CCWWGG 3 cut(s) 306, 740, 1082
TaqI TCGA 7 cut(s) 269, 418, 812, 905, 1146, 1339, 1361
TasI AATT 7 cut(s) 265, 420, 640, 704, 710, 1190, 1301
TfiI GAWTC 9 cut(s) 175, 223, 302, 425, 497, 601, 670, 787, 1059
Tru1I TTAA 3 cut(s) 776, 945, 969
Tru9I TTAA 3 cut(s) 776, 945, 969
TscAI CASTG 1 cut(s) 850
TseFI GTSAC 1 cut(s) 949
TseI GCWGC 2 cut(s) 311, 1428
Tsp45I GTSAC 1 cut(s) 949
TspRI CASTG 1 cut(s) 850
VpaK11BI GGWCC 1 cut(s) 1306
XapI RAATTY 3 cut(s) 265, 710, 1190
XmiI GTMKAC 1 cut(s) 1146
XspI CTAG 3 cut(s) 485, 1109, 1492
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.