MD09G1063500.v1.1

Targeting protein for Xklp2 (TPX2)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0004726
Physical Location & Seq
Forward (+)
4351060 .. 4352800
1741 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1063500.v1.1.491

Sequence Viewer

Length: 393 bp
ATGGAAAGGGGAAGACAAAAGGAGGAAGAGTTCGTGAAGAAGTTACAAGAGATGATGATGGAGGAGAAGAGGCAGCGGATACCAATTGCTCAGGGCCTCCCATGGACAACTGACGAACCAGAGGTGGCAGAGAAGATGAGCCTGTTTGAGCAATACAAGATGGAAAGAGAGAGACTGCAGAAGTTGGCAGAAGAGGAGGAAATAAGAAGATTGAGAAAGGAGCTTGTCCCAAAAGCACAGCCGATGCCCTACTTTGACAGGCCTTTCATTCCCAGAAGGTCAATGAAGCATCCAACTATACCGAAAGAACCGAAGTTTCATGTGCCTCAAAACAAGAAGATCAAGTGCTGCTTGTCATGGAACGATATGAGCTCCTACACTTACGAACATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPX2

Protein Analysis

131

Amino Acids

15.94

Weight (kDa)

9.05

Isoelectric Point (pI)

79.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPX2 PF06886 41 - 99 1.7e-16 Targeting protein for Xklp2 (TPX2) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 76
AloI GAACNNNNNNTCC 2 cut(s) 14, 46
AluBI AGCT 2 cut(s) 223, 372
AluI AGCT 2 cut(s) 223, 372
Alw21I GWGCWC 1 cut(s) 374
Alw26I GTCTC 1 cut(s) 166
AoxI GGCC 2 cut(s) 94, 260
ApeKI GCWGC 2 cut(s) 73, 348
AspS9I GGNCC 1 cut(s) 94
BanII GRGCYC 1 cut(s) 374
BbsI GAAGAC 1 cut(s) 19
Bbv12I GWGCWC 1 cut(s) 374
BbvI GCAGC 2 cut(s) 85, 335
BccI CCATC 2 cut(s) 52, 154
BciVI GTATCC 1 cut(s) 72
BcoDI GTCTC 1 cut(s) 166
BfmI CTRYAG 1 cut(s) 176
BfuI GTATCC 1 cut(s) 72
BisI GCNGC 2 cut(s) 74, 349
BlsI GCNGC 2 cut(s) 75, 350
BmgT120I GGNCC 1 cut(s) 94
BmsI GCATC 2 cut(s) 234, 298
BpiI GAAGAC 1 cut(s) 19
Bpu10I CCTNAGC 1 cut(s) 90
BsaJI CCNNGG 1 cut(s) 101
BsaXI ACNNNNNCTCC 2 cut(s) 14, 44
BseDI CCNNGG 1 cut(s) 101
BseGI GGATG 1 cut(s) 289
BseMII CTCAG 1 cut(s) 104
BseRI GAGGAG 2 cut(s) 77, 209
BseXI GCAGC 2 cut(s) 85, 335
BshFI GGCC 2 cut(s) 96, 262
BsiHKAI GWGCWC 1 cut(s) 374
BslFI GGGAC 1 cut(s) 212
BsmAI GTCTC 1 cut(s) 166
BsmFI GGGAC 1 cut(s) 212
BsnI GGCC 2 cut(s) 96, 262
Bsp1286I GDGCHC 1 cut(s) 374
Bsp143I GATC 1 cut(s) 339
Bsp19I CCATGG 1 cut(s) 101
BspACI CCGC 1 cut(s) 76
BspANI GGCC 2 cut(s) 96, 262
BspCNI CTCAG 1 cut(s) 103
BspMAI CTGCAG 1 cut(s) 180
BssECI CCNNGG 1 cut(s) 101
BssMI GATC 1 cut(s) 339
BssT1I CCWWGG 1 cut(s) 101
Bst6I CTCTTC 3 cut(s) 21, 62, 186
BstDEI CTNAG 1 cut(s) 90
BstDSI CCRYGG 1 cut(s) 101
BstF5I GGATG 1 cut(s) 289
BstKTI GATC 1 cut(s) 342
BstMAI GTCTC 1 cut(s) 166
BstMBI GATC 1 cut(s) 339
BstSFI CTRYAG 1 cut(s) 176
BstV1I GCAGC 2 cut(s) 85, 335
BstV2I GAAGAC 1 cut(s) 19
BsuI GTATCC 1 cut(s) 72
BsuRI GGCC 2 cut(s) 96, 262
BtgI CCRYGG 1 cut(s) 101
BtsCI GGATG 1 cut(s) 289
Cfr13I GGNCC 1 cut(s) 94
CviAII CATG 3 cut(s) 102, 320, 357
CviJI RGCY 6 cut(s) 96, 141, 223, 241, 262, 372
CviKI_1 RGCY 6 cut(s) 96, 141, 223, 241, 262, 372
DdeI CTNAG 1 cut(s) 90
DpnI GATC 1 cut(s) 341
DpnII GATC 1 cut(s) 339
Eam1104I CTCTTC 3 cut(s) 21, 62, 186
EarI CTCTTC 3 cut(s) 21, 62, 186
Ecl136II GAGCTC 1 cut(s) 372
Eco130I CCWWGG 1 cut(s) 101
Eco147I AGGCCT 1 cut(s) 262
Eco24I GRGCYC 1 cut(s) 374
Eco53kI GAGCTC 1 cut(s) 372
EcoICRI GAGCTC 1 cut(s) 372
EcoO109I RGGNCCY 1 cut(s) 94
EcoT14I CCWWGG 1 cut(s) 101
EcoT38I GRGCYC 1 cut(s) 374
ErhI CCWWGG 1 cut(s) 101
FaeI CATG 3 cut(s) 105, 323, 360
FaiI YATR 5 cut(s) 103, 299, 321, 358, 368
FalI AAGNNNNNCTT 2 cut(s) 335, 367
FaqI GGGAC 1 cut(s) 212
FatI CATG 3 cut(s) 101, 319, 356
Fnu4HI GCNGC 2 cut(s) 74, 349
FokI GGATG 1 cut(s) 276
FriOI GRGCYC 1 cut(s) 374
Fsp4HI GCNGC 2 cut(s) 74, 349
GluI GCNGC 2 cut(s) 74, 349
HaeIII GGCC 2 cut(s) 96, 262
Hin1II CATG 3 cut(s) 105, 323, 360
Hpy188III TCNNGA 1 cut(s) 34
HpyAV CCTTC 1 cut(s) 270
HpyCH4V TGCA 1 cut(s) 178
HpyF3I CTNAG 1 cut(s) 90
Hsp92II CATG 3 cut(s) 105, 323, 360
Kzo9I GATC 1 cut(s) 339
LmnI GCTCC 2 cut(s) 220, 377
LpnPI CCDG 5 cut(s) 77, 132, 155, 244, 286
Lsp1109I GCAGC 2 cut(s) 85, 335
LweI GCATC 2 cut(s) 234, 298
MaeIII GTNAC 1 cut(s) 42
MalI GATC 1 cut(s) 341
MboI GATC 1 cut(s) 339
MboII GAAGA 8 cut(s) 24, 38, 49, 79, 145, 203, 219, 349
MfeI CAATTG 1 cut(s) 84
MhlI GDGCHC 1 cut(s) 374
MluCI AATT 1 cut(s) 84
MmeI TCCRAC 1 cut(s) 317
MnlI CCTC 8 cut(s) 16, 55, 63, 107, 115, 187, 190, 336
MspA1I CMGCKG 1 cut(s) 76
MunI CAATTG 1 cut(s) 84
NcoI CCATGG 1 cut(s) 101
NdeII GATC 1 cut(s) 339
NlaIII CATG 3 cut(s) 105, 323, 360
PceI AGGCCT 1 cut(s) 262
PkrI GCNGC 2 cut(s) 75, 350
Psp124BI GAGCTC 1 cut(s) 374
PspPI GGNCC 1 cut(s) 94
PstI CTGCAG 1 cut(s) 180
SacI GAGCTC 1 cut(s) 374
SatI GCNGC 2 cut(s) 74, 349
Sau3AI GATC 1 cut(s) 339
Sau96I GGNCC 1 cut(s) 94
SduI GDGCHC 1 cut(s) 374
SetI ASST 4 cut(s) 126, 225, 281, 374
SfaNI GCATC 2 cut(s) 234, 298
SfcI CTRYAG 1 cut(s) 176
Sse9I AATT 1 cut(s) 84
SseBI AGGCCT 1 cut(s) 262
SsiI CCGC 1 cut(s) 76
SstI GAGCTC 1 cut(s) 374
StuI AGGCCT 1 cut(s) 262
StyI CCWWGG 1 cut(s) 101
TasI AATT 1 cut(s) 84
TseI GCWGC 2 cut(s) 73, 348
TspDTI ATGAA 3 cut(s) 256, 299, 308
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.