pycom17g05710

Targeting protein for Xklp2 (TPX2)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0004726
Physical Location & Seq
Forward (+)
4043206 .. 4045624
2419 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom17g05710.1

Sequence Viewer

Length: 1497 bp
ATGGATTTTATCGGCAAGAACGCCGGAGTGGTGACTCCTGTGAAAGACCAACACGGGTCTCGATACAAGAAGCACGAGAAATCAAGATGCACTGAGAATATGAACCCCAATGTTTCGCCTGGCCCGAAACACACTGCTTCCCCTGCGACGAAATCGGCGGGGAAGTCCCAGAAATCGGCGTCCAAGAACCCGAACCCGGTTGTTCGTTCGCCCCGGAACAAGATCCGGGAGAGGAAGTTCATCGTTGCGAAGAAGAAATCAAAGAAGGAGAACCCGAACGTCTCCTGCAAGTGCAAAGATAAGGGCAATTCGAAGATGTGCCTCTGCGTTGCGTATGAGAATCTGAGGGCGTCGCAGGAAGAGTTCTTCAAGAAGCGAAACGACAACGTCGAATCGGAGAGTTTGAAGGAGAGCGAAAGAGCTGGGCGTGAACTGGAGGAAGAAATCGAGGAGGGGTTGAGGATTCAGGACCTTCAGATTGAAAATGGGTCGGGTGATATTGACCCGGATAGTGAAATGGGTTGTTCGACAATCAAGAGAAGAAGGGACAAGTTGCTGGAAGAGGCGAGGAAGAGCGTGCCGACGAGTGGGAAGGTGATGCATTTGGTTCAGGCGTTTGAGAAGCTGCTTTCGATTCCGAGCTCGAAGGATTCGGATGAGCAGAATGGGGAGGAGGAAGCGGCGGAAGAGATTGGGAAGAAGGCGGCGAAGTGGGCATTGCCCGGATTGCAGCCTCCTCCTAAGGCGCTGGAAACACAGGTCTCGTCTTCTTCGTTTTTCCCGTCGGACTTGTTCTTGACGTCGGAGAATCTCGGTCTGGATCGGCGAACTTCAGTCTCTTCTTCTTGGGATGGAAGTCTGGGAAGTGTCTCAAGCAAGACTTCGAATGGGGGGCGAAGGAGCCGAAGAAATAGCTCTGAGTCTTCCAGCACAATCGGAGGAGGGAGATGGAAGAAGAAGAAGCAACAGAGAGCCACTAGCGCTAAGCCATTCAAGCTAAGAACAGAGGAAAGGGGAAGACAAAAGGAGGAAGAGTTCACGAAGAAGCTACAAGAGATGATGATGGAGGAGGAGAGGCAGCGGATACCAATTGCTCAGGGCCTCCCATGGACAACTGACGAACCAGAGTGCTTACTGAAACCTCCAGTAAAAGAAATAACAATACCAACAGACCTGAAGCTCCACAGTGACATGCGGGCAGTAGAGCGAGCTGAGTTCGACCATCAGGTGGCAGAGAAGATGAGCCTGTTTGAGCAATACAAGATGGAAAGAGAAAGACTGCTGAAGTTGGCAGAAGAGGAGGAAATAAGAAGATTGAAAAAGGAGCTTGTTCCGAAAGCGCAGCCAATGCCCTACTTTGACAGGCCTTTCATTCCCAGAAGGTCAATGAAGCATCCAACCATACCGAAAGAACCGAAGTTTCACGTGCCTCAAAACAAGAAGATCAAATGTTACTTGTCCTGGAACGAGATGAGCACCTACACTTACGAACATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPX2

Protein Analysis

499

Amino Acids

56.82

Weight (kDa)

9.4

Isoelectric Point (pI)

67.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPX2 PF06886 393 - 467 2.7e-23 Targeting protein for Xklp2 (TPX2) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 803
AccB7I CCANNNNNTGG 1 cut(s) 1228
AciI CCGC 6 cut(s) 158, 680, 683, 704, 1081, 1195
AclWI GGATC 2 cut(s) 217, 828
AcuI CTGAAG 4 cut(s) 458, 816, 1196, 1304
AcvI CACGTG 1 cut(s) 1426
AcyI GRCGYC 3 cut(s) 179, 350, 800
AfeI AGCGCT 1 cut(s) 982
AfiI CCNNNNNNNGG 4 cut(s) 175, 196, 587, 1228
AgsI TTSAA 5 cut(s) 370, 406, 482, 994, 1318
AjnI CCWGG 2 cut(s) 118, 1460
AloI GAACNNNNNNTCC 4 cut(s) 221, 253, 1019, 1051
AluBI AGCT 9 cut(s) 422, 625, 642, 915, 997, 1048, 1180, 1211, 1327
AluI AGCT 9 cut(s) 422, 625, 642, 915, 997, 1048, 1180, 1211, 1327
Alw21I GWGCWC 2 cut(s) 644, 1478
Alw26I GTCTC 5 cut(s) 63, 286, 766, 841, 874
AlwI GGATC 2 cut(s) 217, 828
Aor51HI AGCGCT 1 cut(s) 982
AoxI GGCC 3 cut(s) 121, 1099, 1364
ApeKI GCWGC 4 cut(s) 625, 730, 1078, 1342
AspLEI GCGC 3 cut(s) 748, 983, 1342
AspS9I GGNCC 3 cut(s) 122, 469, 1099
AsuC2I CCSGG 5 cut(s) 197, 214, 227, 506, 723
AsuHPI GGTGA 3 cut(s) 43, 506, 607
AsuII TTCGAA 2 cut(s) 311, 884
AvaII GGWCC 1 cut(s) 469
AxyI CCTNAGG 1 cut(s) 741
BanII GRGCYC 1 cut(s) 644
BauI CACGAG 1 cut(s) 74
BbrPI CACGTG 1 cut(s) 1426
BbsI GAAGAC 3 cut(s) 759, 915, 1024
Bbv12I GWGCWC 2 cut(s) 644, 1478
BbvI GCAGC 4 cut(s) 612, 742, 1090, 1354
BccI CCATC 5 cut(s) 845, 942, 1057, 1230, 1258
BciT130I CCWGG 2 cut(s) 120, 1462
BciVI GTATCC 1 cut(s) 1077
BcnI CCSGG 5 cut(s) 197, 214, 227, 506, 723
BcoDI GTCTC 5 cut(s) 63, 286, 766, 841, 874
BfaI CTAG 1 cut(s) 978
BfoI RGCGCY 2 cut(s) 749, 984
BfuI GTATCC 1 cut(s) 1077
BisI GCNGC 6 cut(s) 626, 681, 705, 731, 1079, 1343
BlpI GCTNAGC 1 cut(s) 984
BlsI GCNGC 6 cut(s) 627, 682, 706, 732, 1080, 1344
Bme1390I CCNGG 7 cut(s) 120, 197, 214, 227, 506, 723, 1462
Bme18I GGWCC 1 cut(s) 469
BmgT120I GGNCC 3 cut(s) 122, 469, 1099
BmiI GGNNCC 1 cut(s) 902
BmrFI CCNGG 7 cut(s) 120, 197, 214, 227, 506, 723, 1462
BmsI GCATC 3 cut(s) 77, 588, 1402
BpiI GAAGAC 3 cut(s) 759, 915, 1024
BpmI CTGGAG 2 cut(s) 455, 1128
Bpu10I CCTNAGC 1 cut(s) 1095
Bpu1102I GCTNAGC 1 cut(s) 984
Bpu14I TTCGAA 2 cut(s) 311, 884
BpuEI CTTGAG 1 cut(s) 856
BpuMI CCSGG 5 cut(s) 197, 214, 227, 506, 723
BsaAI YACGTR 1 cut(s) 1426
BsaHI GRCGYC 3 cut(s) 179, 350, 800
BsaI GGTCTC 2 cut(s) 63, 766
BsaJI CCNNGG 2 cut(s) 212, 1106
BsaXI ACNNNNNCTCC 4 cut(s) 221, 251, 1019, 1049
Bsc4I CCNNNNNNNGG 4 cut(s) 175, 196, 587, 1228
Bse1I ACTGG 2 cut(s) 438, 1145
Bse21I CCTNAGG 1 cut(s) 741
Bse3DI GCAATG 1 cut(s) 716
BseBI CCWGG 2 cut(s) 120, 1462
BseDI CCNNGG 2 cut(s) 212, 1106
BseGI GGATG 3 cut(s) 661, 856, 1393
BseLI CCNNNNNNNGG 4 cut(s) 175, 196, 587, 1228
BseMI GCAATG 1 cut(s) 716
BseMII CTCAG 5 cut(s) 84, 335, 909, 1109, 1203
BseNI ACTGG 2 cut(s) 438, 1145
BseRI GAGGAG 7 cut(s) 464, 686, 726, 954, 1082, 1085, 1313
BseXI GCAGC 4 cut(s) 612, 742, 1090, 1354
BseYI CCCAGC 1 cut(s) 422
BshFI GGCC 3 cut(s) 123, 1101, 1366
BsiHKAI GWGCWC 2 cut(s) 644, 1478
BsiSI CCGG 6 cut(s) 24, 197, 214, 226, 506, 723
BslFI GGGAC 2 cut(s) 151, 560
BslI CCNNNNNNNGG 4 cut(s) 175, 196, 587, 1228
BsmAI GTCTC 5 cut(s) 63, 286, 766, 841, 874
BsmBI CGTCTC 1 cut(s) 286
BsmFI GGGAC 2 cut(s) 151, 560
BsnI GGCC 3 cut(s) 123, 1101, 1366
Bso31I GGTCTC 2 cut(s) 63, 766
Bsp119I TTCGAA 2 cut(s) 311, 884
Bsp1286I GDGCHC 2 cut(s) 644, 1478
Bsp143I GATC 3 cut(s) 222, 820, 1443
Bsp1720I GCTNAGC 1 cut(s) 984
Bsp19I CCATGG 1 cut(s) 1106
BspACI CCGC 6 cut(s) 158, 680, 683, 704, 1081, 1195
BspANI GGCC 3 cut(s) 123, 1101, 1366
BspCNI CTCAG 5 cut(s) 85, 336, 910, 1108, 1204
BspLI GGNNCC 1 cut(s) 902
BspPI GGATC 2 cut(s) 217, 828
BspQI GCTCTTC 1 cut(s) 566
BspT104I TTCGAA 2 cut(s) 311, 884
BspTNI GGTCTC 2 cut(s) 63, 766
BsrDI GCAATG 1 cut(s) 716
BsrI ACTGG 2 cut(s) 438, 1145
BssECI CCNNGG 2 cut(s) 212, 1106
BssMI GATC 3 cut(s) 222, 820, 1443
BssNI GRCGYC 3 cut(s) 179, 350, 800
BssSI CACGAG 1 cut(s) 74
BssT1I CCWWGG 1 cut(s) 1106
Bst2BI CACGAG 1 cut(s) 74
Bst2UI CCWGG 2 cut(s) 120, 1462
Bst4CI ACNGT 1 cut(s) 1187
Bst6I CTCTTC 7 cut(s) 354, 555, 566, 681, 844, 1026, 1290
BstACI GRCGYC 3 cut(s) 179, 350, 800
BstAPI GCANNNNNTGC 1 cut(s) 1348
BstBAI YACGTR 1 cut(s) 1426
BstBI TTCGAA 2 cut(s) 311, 884
BstC8I GCNNGC 3 cut(s) 578, 1197, 1209
BstDEI CTNAG 8 cut(s) 93, 344, 741, 918, 984, 998, 1095, 1212
BstDSI CCRYGG 1 cut(s) 1106
BstF5I GGATG 3 cut(s) 661, 856, 1393
BstH2I RGCGCY 2 cut(s) 749, 984
BstHHI GCGC 3 cut(s) 748, 983, 1342
BstKTI GATC 3 cut(s) 225, 823, 1446
BstMAI GTCTC 5 cut(s) 63, 286, 766, 841, 874
BstMBI GATC 3 cut(s) 222, 820, 1443
BstMWI GCNNNNNNNGC 6 cut(s) 143, 713, 727, 980, 994, 1348
BstNI CCWGG 2 cut(s) 120, 1462
BstNSI RCATGY 1 cut(s) 1195
BstSCI CCNGG 7 cut(s) 118, 195, 212, 225, 504, 721, 1460
BstV1I GCAGC 4 cut(s) 612, 742, 1090, 1354
BstV2I GAAGAC 3 cut(s) 759, 915, 1024
BstX2I RGATCY 1 cut(s) 222
BstYI RGATCY 1 cut(s) 222
Bsu36I CCTNAGG 1 cut(s) 741
BsuI GTATCC 1 cut(s) 1077
BsuRI GGCC 3 cut(s) 123, 1101, 1366
BtgI CCRYGG 1 cut(s) 1106
BtsCI GGATG 3 cut(s) 661, 856, 1393
BtsI GCAGTG 1 cut(s) 132
BtsIMutI CAGTG 3 cut(s) 90, 132, 1192
Cac8I GCNNGC 3 cut(s) 578, 1197, 1209
CfoI GCGC 3 cut(s) 748, 983, 1342
Cfr13I GGNCC 3 cut(s) 122, 469, 1099
CseI GACGC 2 cut(s) 168, 339
CviAII CATG 2 cut(s) 1107, 1192
DdeI CTNAG 8 cut(s) 93, 344, 741, 918, 984, 998, 1095, 1212
DpnI GATC 3 cut(s) 224, 822, 1445
DpnII GATC 3 cut(s) 222, 820, 1443
Eam1104I CTCTTC 7 cut(s) 354, 555, 566, 681, 844, 1026, 1290
EarI CTCTTC 7 cut(s) 354, 555, 566, 681, 844, 1026, 1290
EciI GGCGGA 1 cut(s) 698
Ecl136II GAGCTC 1 cut(s) 642
Eco130I CCWWGG 1 cut(s) 1106
Eco147I AGGCCT 1 cut(s) 1366
Eco24I GRGCYC 1 cut(s) 644
Eco31I GGTCTC 2 cut(s) 63, 766
Eco47I GGWCC 1 cut(s) 469
Eco47III AGCGCT 1 cut(s) 982
Eco53kI GAGCTC 1 cut(s) 642
Eco57I CTGAAG 4 cut(s) 458, 816, 1196, 1304
Eco72I CACGTG 1 cut(s) 1426
Eco81I CCTNAGG 1 cut(s) 741
EcoICRI GAGCTC 1 cut(s) 642
EcoO109I RGGNCCY 2 cut(s) 469, 1099
EcoRII CCWGG 2 cut(s) 118, 1460
EcoT14I CCWWGG 1 cut(s) 1106
EcoT22I ATGCAT 1 cut(s) 603
EcoT38I GRGCYC 1 cut(s) 644
ErhI CCWWGG 1 cut(s) 1106
Esp3I CGTCTC 1 cut(s) 286
FaeI CATG 2 cut(s) 1110, 1195
FaiI YATR 5 cut(s) 101, 336, 1108, 1193, 1403
FalI AAGNNNNNCTT 2 cut(s) 865, 897
FaqI GGGAC 2 cut(s) 151, 560
FatI CATG 2 cut(s) 1106, 1191
FauI CCCGC 2 cut(s) 151, 1188
Fnu4HI GCNGC 6 cut(s) 626, 681, 705, 731, 1079, 1343
FokI GGATG 3 cut(s) 668, 863, 1380
FriOI GRGCYC 1 cut(s) 644
Fsp4HI GCNGC 6 cut(s) 626, 681, 705, 731, 1079, 1343
FspBI CTAG 1 cut(s) 978
GlaI GCGC 3 cut(s) 747, 982, 1341
GluI GCNGC 6 cut(s) 626, 681, 705, 731, 1079, 1343
GsaI CCCAGC 1 cut(s) 426
GsuI CTGGAG 2 cut(s) 455, 1128
HaeII RGCGCY 2 cut(s) 749, 984
HaeIII GGCC 3 cut(s) 123, 1101, 1366
HapII CCGG 6 cut(s) 24, 197, 214, 226, 506, 723
HgaI GACGC 2 cut(s) 168, 339
HhaI GCGC 3 cut(s) 748, 983, 1342
Hin1I GRCGYC 3 cut(s) 179, 350, 800
Hin1II CATG 2 cut(s) 1110, 1195
Hin6I GCGC 3 cut(s) 746, 981, 1340
HinP1I GCGC 3 cut(s) 746, 981, 1340
HinfI GANTC 8 cut(s) 34, 340, 392, 463, 634, 650, 808, 920
HpaII CCGG 6 cut(s) 24, 197, 214, 226, 506, 723
HphI GGTGA 3 cut(s) 43, 506, 607
Hpy166II GTNNAC 2 cut(s) 431, 1038
Hpy188III TCNNGA 8 cut(s) 60, 84, 370, 467, 535, 796, 818, 1039
Hpy8I GTNNAC 2 cut(s) 431, 1038
Hpy99I CGWCG 6 cut(s) 151, 355, 392, 586, 787, 805
HpyAV CCTTC 9 cut(s) 259, 400, 482, 537, 586, 640, 694, 891, 1374
HpyCH4III ACNGT 1 cut(s) 1187
HpyCH4IV ACGT 4 cut(s) 279, 387, 800, 1425
HpyCH4V TGCA 5 cut(s) 90, 288, 294, 601, 730
HpyF10VI GCNNNNNNNGC 6 cut(s) 143, 713, 727, 980, 994, 1348
HpyF3I CTNAG 8 cut(s) 93, 344, 741, 918, 984, 998, 1095, 1212
HpySE526I ACGT 4 cut(s) 279, 387, 800, 1425
Hsp92I GRCGYC 3 cut(s) 179, 350, 800
Hsp92II CATG 2 cut(s) 1110, 1195
HspAI GCGC 3 cut(s) 746, 981, 1340
Kzo9I GATC 3 cut(s) 222, 820, 1443
LguI GCTCTTC 1 cut(s) 566
LmnI GCTCC 3 cut(s) 900, 1185, 1324
Lsp1109I GCAGC 4 cut(s) 612, 742, 1090, 1354
LweI GCATC 3 cut(s) 77, 588, 1402
MaeI CTAG 1 cut(s) 978
MaeII ACGT 4 cut(s) 279, 387, 800, 1425
MaeIII GTNAC 3 cut(s) 31, 1187, 1451
MalI GATC 3 cut(s) 224, 822, 1445
MboI GATC 3 cut(s) 222, 820, 1443
MfeI CAATTG 1 cut(s) 1089
MflI RGATCY 1 cut(s) 222
MhlI GDGCHC 2 cut(s) 644, 1478
MluCI AATT 2 cut(s) 307, 1089
MlyI GAGTC 2 cut(s) 28, 929
MmeI TCCRAC 3 cut(s) 765, 783, 1421
Mph1103I ATGCAT 1 cut(s) 603
MspA1I CMGCKG 1 cut(s) 1081
MspI CCGG 6 cut(s) 24, 197, 214, 226, 506, 723
MspR9I CCNGG 7 cut(s) 120, 197, 214, 227, 506, 723, 1462
MunI CAATTG 1 cut(s) 1089
MvaI CCWGG 2 cut(s) 120, 1462
MwoI GCNNNNNNNGC 6 cut(s) 143, 713, 727, 980, 994, 1348
NciI CCSGG 5 cut(s) 197, 214, 227, 506, 723
NcoI CCATGG 1 cut(s) 1106
NdeII GATC 3 cut(s) 222, 820, 1443
NlaIII CATG 2 cut(s) 1110, 1195
NlaIV GGNNCC 1 cut(s) 902
NmuCI GTSAC 2 cut(s) 31, 1187
NsiI ATGCAT 1 cut(s) 603
NspI RCATGY 1 cut(s) 1195
NspV TTCGAA 2 cut(s) 311, 884
PceI AGGCCT 1 cut(s) 1366
PciSI GCTCTTC 1 cut(s) 566
PcsI WCGNNNNNNNCGW 4 cut(s) 122, 387, 770, 779
PfeI GAWTC 6 cut(s) 340, 392, 463, 634, 650, 808
PflFI GACNNNGTC 1 cut(s) 386
PflMI CCANNNNNTGG 1 cut(s) 1228
PfoI TCCNGGA 2 cut(s) 225, 1460
PkrI GCNGC 6 cut(s) 627, 682, 706, 732, 1080, 1344
PleI GAGTC 2 cut(s) 28, 928
PmaCI CACGTG 1 cut(s) 1426
PmlI CACGTG 1 cut(s) 1426
PpsI GAGTC 2 cut(s) 28, 928
Ppu21I YACGTR 1 cut(s) 1426
PpuMI RGGWCCY 1 cut(s) 469
Psp124BI GAGCTC 1 cut(s) 644
Psp5II RGGWCCY 1 cut(s) 469
Psp6I CCWGG 2 cut(s) 118, 1460
PspCI CACGTG 1 cut(s) 1426
PspFI CCCAGC 1 cut(s) 422
PspGI CCWGG 2 cut(s) 118, 1460
PspN4I GGNNCC 1 cut(s) 902
PspPI GGNCC 3 cut(s) 122, 469, 1099
PspPPI RGGWCCY 1 cut(s) 469
PsuI RGATCY 1 cut(s) 222
PsyI GACNNNGTC 1 cut(s) 386
SacI GAGCTC 1 cut(s) 644
SapI GCTCTTC 1 cut(s) 566
SatI GCNGC 6 cut(s) 626, 681, 705, 731, 1079, 1343
Sau3AI GATC 3 cut(s) 222, 820, 1443
Sau96I GGNCC 3 cut(s) 122, 469, 1099
SchI GAGTC 2 cut(s) 28, 929
ScrFI CCNGG 7 cut(s) 120, 197, 214, 227, 506, 723, 1462
SduI GDGCHC 2 cut(s) 644, 1478
SfaNI GCATC 3 cut(s) 77, 588, 1402
SfuI TTCGAA 2 cut(s) 311, 884
SinI GGWCC 1 cut(s) 469
SmlI CTYRAG 1 cut(s) 871
SmoI CTYRAG 1 cut(s) 871
Sse9I AATT 2 cut(s) 307, 1089
SseBI AGGCCT 1 cut(s) 1366
SsiI CCGC 6 cut(s) 158, 680, 683, 704, 1081, 1195
SspMI CTAG 1 cut(s) 978
SstI GAGCTC 1 cut(s) 644
StuI AGGCCT 1 cut(s) 1366
StyD4I CCNGG 7 cut(s) 118, 195, 212, 225, 504, 721, 1460
StyI CCWWGG 1 cut(s) 1106
TaaI ACNGT 1 cut(s) 1187
TaiI ACGT 4 cut(s) 282, 390, 803, 1428
TaqI TCGA 9 cut(s) 61, 311, 390, 447, 527, 632, 644, 884, 1218
TaqII GACCGA 1 cut(s) 803
TasI AATT 2 cut(s) 307, 1089
TauI GCSGC 2 cut(s) 683, 707
TfiI GAWTC 6 cut(s) 340, 392, 463, 634, 650, 808
TscAI CASTG 3 cut(s) 97, 139, 1192
TseFI GTSAC 2 cut(s) 31, 1187
TseI GCWGC 4 cut(s) 625, 730, 1078, 1342
Tsp45I GTSAC 2 cut(s) 31, 1187
TspDTI ATGAA 4 cut(s) 116, 229, 1360, 1403
TspRI CASTG 3 cut(s) 97, 139, 1192
Tth111I GACNNNGTC 1 cut(s) 386
Van91I CCANNNNNTGG 1 cut(s) 1228
VpaK11BI GGWCC 1 cut(s) 469
XceI RCATGY 1 cut(s) 1195
XspI CTAG 1 cut(s) 978
ZraI GACGTC 1 cut(s) 801
Zsp2I ATGCAT 1 cut(s) 603
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.