Rroxscaffold_2G00085110

Targeting protein for Xklp2 (TPX2)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0004726
Physical Location & Seq
Forward (+)
7502110 .. 7505667
3558 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00085110.1

Sequence Viewer

Length: 1455 bp
ATGGACTCGGTCGGCAAGAGCGCCGGAGCGGTGACTCCGATCAAAGACCAACACGGCTCCCGATCCAAGAAGCAAGAAAACTCCAGATACTCCGAGAATCTGAACCCCAACGTCTCTCCCGGCCCGAAATCGGCCAAGTCCCAGAAATCGGGTTCCAAGAACCCGACCAACCCGAACCCGGTTTTCCAGTCGCCCCGGAAGAAGATCCGGGAGAGGAAATTCGTGGTGGCGAAGAAGAGAGGGAAGAAGGAGGAAAATCCGAACCCGAATCCGAATTCGAACGCCGAGTGCAGCAAGTGCAAAGAGAAAGGCGGCGATTCGAAGAAGAAGTGTCTGTGTTTGGCTTACGAGAATCTGAGGGCTTCTCAGGAGGAGTTCTTTAAGACCCGACCCGAAACCGAAAACGGGTCGAAGGAGTCCGACAGGGCCGATGATCGGGAACTCGAGGAGGCCTTGCTGATTCAGGACCAGGACTTCGACGATCGCGACCCGGGAGAGCCCGACCCGACTGACGAAACGGGTACGATTAAGAGGAGGAGGGCGAGGCTGTTGGAGGAGGCGAGGAAGAGCGTGCCGGCGCGTGGGAAGGTGATGCACTTGGTTCAGGCGTTTGAGAAGCTGCTCACGATTCCCAAGGAAGGAGACGAAGCAGAGGAAGAAGAGAGTGGTAAGAAGGCTACGAAGTGGGCCTTGCCTGGGCTGCAACCGCCGGAGGCCCATGAGTCGTCGTCGTCGTCGTTTTGCCCGTCGGATTTGTTTCTGACGTCGGAGAATCTCGGGCTGGATCGCCGGCCGTCGTTGTCGTCGTCTTGGGATGGAAGCATTTCGAACAGGACGTCGAATGGGGGAGGAGAAGCCGAAGAAATAGCTCGGAGTCGTCTGGCACGATTGGAGGAAGTAGATGGAAGAAGAAGCAGCAGCTCAGAGCAACTAGCCTTAAACCGTTCAAGCTCAGGACAGAGGGGGAGAATGAAGGGAGAAGAGTTCATGAAGAAGGTACAGGAGATGATAATACAGGAGGAGAGGCAGCGGATACCGATTGCTCAGGGCCTCCCATGGACAACAGATGAACCAGAGTGCTTAATAAAACCTCCTGTAAAAGAGAGCACAAGACCAACAGACCTGAAGCTCCACACTGACATGCGGGCAGTAGAGCGTGCTGAGTTTGATCATCAGGTGGCAGAGAAGCTGAGCCTGTTTGAGCAATACAAGATGGAAAGAGAGAGACTGCAGAAGTTGGCAGAAGAGGAAGAAGTAAGAAGGCTGAGAAAGGAGCTTGTTCCAAAAGCACAGCCAATGCCCTACTTTGACAGGCCTTTCATTCCCAGAAGGTCAATGAAGCATCCAACCATACCCAAAGACCCAAAGTTCCATATCCCTCAACATAAGAAGATTAAGTGTTGCTTGTCATGGAATGATGATATGAGCTCCTACTCTTATCAAAGTGAAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPX2

Protein Analysis

484

Amino Acids

55.0

Weight (kDa)

8.77

Isoelectric Point (pI)

62.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPX2 PF06886 376 - 450 3.6e-24 Targeting protein for Xklp2 (TPX2) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 767, 839
AccBSI CCGCTC 1 cut(s) 29
AccII CGCG 2 cut(s) 486, 580
AciI CCGC 5 cut(s) 29, 312, 707, 1030, 1144
AclWI GGATC 3 cut(s) 57, 199, 792
AcoI YGGCCR 2 cut(s) 132, 791
AcsI RAATTY 2 cut(s) 218, 274
AcuI CTGAAG 1 cut(s) 1145
AcyI GRCGYC 2 cut(s) 764, 836
AfaI GTAC 2 cut(s) 523, 999
AfiI CCNNNNNNNGG 8 cut(s) 130, 148, 178, 405, 435, 581, 638, 696
AgsI TTSAA 1 cut(s) 948
AjnI CCWGG 2 cut(s) 468, 694
AluBI AGCT 9 cut(s) 619, 869, 921, 951, 1129, 1189, 1276, 1428, 1451
AluI AGCT 9 cut(s) 619, 869, 921, 951, 1129, 1189, 1276, 1428, 1451
Alw21I GWGCWC 2 cut(s) 1109, 1430
Alw26I GTCTC 3 cut(s) 118, 636, 1219
AlwI GGATC 3 cut(s) 57, 199, 792
Ama87I CYCGRG 3 cut(s) 443, 490, 776
AoxI GGCC 9 cut(s) 121, 132, 426, 450, 687, 714, 791, 1048, 1313
ApeKI GCWGC 6 cut(s) 291, 619, 700, 915, 918, 1027
ApoI RAATTY 2 cut(s) 218, 274
ArsI GACNNNNNNTTYG 4 cut(s) 458, 490, 821, 853
Asp700I GAANNNNTTC 1 cut(s) 823
AspLEI GCGC 2 cut(s) 23, 580
AspS9I GGNCC 6 cut(s) 122, 426, 466, 687, 715, 1048
AsuC2I CCSGG 6 cut(s) 120, 179, 196, 209, 491, 492
AsuHPI GGTGA 2 cut(s) 43, 601
AsuII TTCGAA 3 cut(s) 278, 320, 827
AvaI CYCGRG 3 cut(s) 443, 490, 776
AvaII GGWCC 1 cut(s) 466
BanII GRGCYC 2 cut(s) 501, 1430
Bbv12I GWGCWC 2 cut(s) 1109, 1430
BbvI GCAGC 6 cut(s) 303, 606, 687, 927, 930, 1039
BccI CCATC 3 cut(s) 809, 896, 1207
BceAI ACGGC 2 cut(s) 70, 778
BciT130I CCWGG 2 cut(s) 470, 696
BciVI GTATCC 1 cut(s) 1026
BclI TGATCA 1 cut(s) 1168
BcnI CCSGG 6 cut(s) 120, 179, 196, 209, 491, 492
BcoDI GTCTC 3 cut(s) 118, 636, 1219
BfaI CTAG 1 cut(s) 932
BfmI CTRYAG 1 cut(s) 1229
BfoI RGCGCY 1 cut(s) 24
BfuI GTATCC 1 cut(s) 1026
BisI GCNGC 7 cut(s) 292, 313, 620, 701, 916, 919, 1028
BlpI GCTNAGC 1 cut(s) 1190
BlsI GCNGC 7 cut(s) 293, 314, 621, 702, 917, 920, 1029
Bme1390I CCNGG 8 cut(s) 120, 179, 196, 209, 470, 491, 492, 696
Bme18I GGWCC 1 cut(s) 466
BmeT110I CYCGRG 3 cut(s) 443, 490, 776
BmgT120I GGNCC 6 cut(s) 122, 426, 466, 687, 715, 1048
BmiI GGNNCC 2 cut(s) 58, 154
BmrFI CCNGG 8 cut(s) 120, 179, 196, 209, 470, 491, 492, 696
BmsI GCATC 2 cut(s) 582, 1351
BplI GAGNNNNNCTC 2 cut(s) 349, 381
BpmI CTGGAG 1 cut(s) 67
Bpu10I CCTNAGC 2 cut(s) 952, 1044
Bpu1102I GCTNAGC 1 cut(s) 1190
Bpu14I TTCGAA 3 cut(s) 278, 320, 827
BpuMI CCSGG 6 cut(s) 120, 179, 196, 209, 491, 492
BsaHI GRCGYC 2 cut(s) 764, 836
BsaJI CCNNGG 5 cut(s) 194, 490, 633, 695, 1055
Bsc4I CCNNNNNNNGG 8 cut(s) 130, 148, 178, 405, 435, 581, 638, 696
Bse118I RCCGGY 2 cut(s) 574, 789
Bse1I ACTGG 1 cut(s) 187
BseBI CCWGG 2 cut(s) 470, 696
BseDI CCNNGG 5 cut(s) 194, 490, 633, 695, 1055
BseGI GGATG 2 cut(s) 820, 1342
BseLI CCNNNNNNNGG 8 cut(s) 130, 148, 178, 405, 435, 581, 638, 696
BseMII CTCAG 8 cut(s) 347, 380, 936, 966, 1058, 1152, 1181, 1256
BseNI ACTGG 1 cut(s) 187
BseRI GAGGAG 7 cut(s) 386, 461, 547, 550, 569, 864, 1034
BseX3I CGGCCG 1 cut(s) 791
BseXI GCAGC 6 cut(s) 303, 606, 687, 927, 930, 1039
BsgI GTGCAG 1 cut(s) 310
Bsh1236I CGCG 2 cut(s) 486, 580
Bsh1285I CGRYCG 3 cut(s) 12, 484, 794
BshFI GGCC 9 cut(s) 123, 134, 428, 452, 689, 716, 793, 1050, 1315
BsiEI CGRYCG 3 cut(s) 12, 484, 794
BsiHKAI GWGCWC 2 cut(s) 1109, 1430
BsiHKCI CYCGRG 3 cut(s) 443, 490, 776
BsiSI CCGG 9 cut(s) 24, 120, 179, 196, 208, 491, 575, 710, 790
BslFI GGGAC 1 cut(s) 124
BslI CCNNNNNNNGG 8 cut(s) 130, 148, 178, 405, 435, 581, 638, 696
BsmAI GTCTC 3 cut(s) 118, 636, 1219
BsmBI CGTCTC 2 cut(s) 118, 636
BsmFI GGGAC 1 cut(s) 124
BsnI GGCC 9 cut(s) 123, 134, 428, 452, 689, 716, 793, 1050, 1315
BsoBI CYCGRG 3 cut(s) 443, 490, 776
Bsp119I TTCGAA 3 cut(s) 278, 320, 827
Bsp1286I GDGCHC 3 cut(s) 501, 1109, 1430
Bsp143I GATC 7 cut(s) 39, 62, 204, 433, 481, 784, 1168
Bsp1720I GCTNAGC 1 cut(s) 1190
Bsp19I CCATGG 1 cut(s) 1055
Bsp68I TCGCGA 1 cut(s) 486
BspACI CCGC 5 cut(s) 29, 312, 707, 1030, 1144
BspANI GGCC 9 cut(s) 123, 134, 428, 452, 689, 716, 793, 1050, 1315
BspCNI CTCAG 8 cut(s) 348, 379, 935, 965, 1057, 1153, 1182, 1257
BspFNI CGCG 2 cut(s) 486, 580
BspHI TCATGA 1 cut(s) 987
BspLI GGNNCC 2 cut(s) 58, 154
BspMAI CTGCAG 1 cut(s) 1233
BspPI GGATC 3 cut(s) 57, 199, 792
BspQI GCTCTTC 1 cut(s) 560
BspT104I TTCGAA 3 cut(s) 278, 320, 827
BsrBI CCGCTC 1 cut(s) 29
BsrFI RCCGGY 2 cut(s) 574, 789
BsrI ACTGG 1 cut(s) 187
BssAI RCCGGY 2 cut(s) 574, 789
BssECI CCNNGG 5 cut(s) 194, 490, 633, 695, 1055
BssMI GATC 7 cut(s) 39, 62, 204, 433, 481, 784, 1168
BssNI GRCGYC 2 cut(s) 764, 836
BssT1I CCWWGG 2 cut(s) 633, 1055
Bst2UI CCWGG 2 cut(s) 470, 696
Bst4CI ACNGT 1 cut(s) 944
Bst6I CTCTTC 5 cut(s) 230, 560, 654, 975, 1239
BstACI GRCGYC 2 cut(s) 764, 836
BstAPI GCANNNNNTGC 1 cut(s) 297
BstBI TTCGAA 3 cut(s) 278, 320, 827
BstC8I GCNNGC 5 cut(s) 572, 576, 791, 1146, 1158
BstDEI CTNAG 8 cut(s) 356, 366, 922, 952, 1044, 1161, 1190, 1265
BstDSI CCRYGG 1 cut(s) 1055
BstF5I GGATG 2 cut(s) 820, 1342
BstFNI CGCG 2 cut(s) 486, 580
BstH2I RGCGCY 1 cut(s) 24
BstHHI GCGC 2 cut(s) 23, 580
BstKTI GATC 7 cut(s) 42, 65, 207, 436, 484, 787, 1171
BstMAI GTCTC 3 cut(s) 118, 636, 1219
BstMBI GATC 7 cut(s) 39, 62, 204, 433, 481, 784, 1168
BstMCI CGRYCG 3 cut(s) 12, 484, 794
BstMWI GCNNNNNNNGC 3 cut(s) 297, 700, 706
BstNI CCWGG 2 cut(s) 470, 696
BstNSI RCATGY 1 cut(s) 1144
BstSCI CCNGG 8 cut(s) 118, 177, 194, 207, 468, 489, 490, 694
BstSFI CTRYAG 1 cut(s) 1229
BstUI CGCG 2 cut(s) 486, 580
BstV1I GCAGC 6 cut(s) 303, 606, 687, 927, 930, 1039
BstX2I RGATCY 1 cut(s) 204
BstYI RGATCY 1 cut(s) 204
BstZI CGGCCG 1 cut(s) 791
BsuI GTATCC 1 cut(s) 1026
BsuRI GGCC 9 cut(s) 123, 134, 428, 452, 689, 716, 793, 1050, 1315
BtgI CCRYGG 1 cut(s) 1055
BtsCI GGATG 2 cut(s) 820, 1342
BtsIMutI CAGTG 1 cut(s) 1134
BtuMI TCGCGA 1 cut(s) 486
Cac8I GCNNGC 5 cut(s) 572, 576, 791, 1146, 1158
CciI TCATGA 1 cut(s) 987
CfoI GCGC 2 cut(s) 23, 580
Cfr10I RCCGGY 2 cut(s) 574, 789
Cfr13I GGNCC 6 cut(s) 122, 426, 466, 687, 715, 1048
Cfr9I CCCGGG 1 cut(s) 490
Csp6I GTAC 2 cut(s) 522, 998
CviAII CATG 5 cut(s) 719, 988, 1056, 1141, 1410
CviQI GTAC 2 cut(s) 522, 998
DdeI CTNAG 8 cut(s) 356, 366, 922, 952, 1044, 1161, 1190, 1265
DpnI GATC 7 cut(s) 41, 64, 206, 435, 483, 786, 1170
DpnII GATC 7 cut(s) 39, 62, 204, 433, 481, 784, 1168
EaeI YGGCCR 2 cut(s) 132, 791
EagI CGGCCG 1 cut(s) 791
Eam1104I CTCTTC 5 cut(s) 230, 560, 654, 975, 1239
EarI CTCTTC 5 cut(s) 230, 560, 654, 975, 1239
Ecl136II GAGCTC 1 cut(s) 1428
EclXI CGGCCG 1 cut(s) 791
Eco130I CCWWGG 2 cut(s) 633, 1055
Eco147I AGGCCT 2 cut(s) 452, 1315
Eco24I GRGCYC 2 cut(s) 501, 1430
Eco47I GGWCC 1 cut(s) 466
Eco52I CGGCCG 1 cut(s) 791
Eco53kI GAGCTC 1 cut(s) 1428
Eco57I CTGAAG 1 cut(s) 1145
Eco88I CYCGRG 3 cut(s) 443, 490, 776
EcoICRI GAGCTC 1 cut(s) 1428
EcoO109I RGGNCCY 1 cut(s) 1048
EcoRI GAATTC 1 cut(s) 274
EcoRII CCWGG 2 cut(s) 468, 694
EcoT14I CCWWGG 2 cut(s) 633, 1055
EcoT38I GRGCYC 2 cut(s) 501, 1430
ErhI CCWWGG 2 cut(s) 633, 1055
Esp3I CGTCTC 2 cut(s) 118, 636
FaeI CATG 5 cut(s) 722, 991, 1059, 1144, 1413
FaiI YATR 9 cut(s) 720, 989, 1057, 1142, 1352, 1374, 1386, 1411, 1424
FalI AAGNNNNNCTT 5 cut(s) 674, 706, 1388, 1420, 1435
FaqI GGGAC 1 cut(s) 124
FatI CATG 5 cut(s) 718, 987, 1055, 1140, 1409
FauI CCCGC 1 cut(s) 1137
FbaI TGATCA 1 cut(s) 1168
Fnu4HI GCNGC 7 cut(s) 292, 313, 620, 701, 916, 919, 1028
FokI GGATG 2 cut(s) 827, 1329
FriOI GRGCYC 2 cut(s) 501, 1430
Fsp4HI GCNGC 7 cut(s) 292, 313, 620, 701, 916, 919, 1028
FspBI CTAG 1 cut(s) 932
GlaI GCGC 2 cut(s) 22, 579
GluI GCNGC 7 cut(s) 292, 313, 620, 701, 916, 919, 1028
GsuI CTGGAG 1 cut(s) 67
HaeII RGCGCY 1 cut(s) 24
HaeIII GGCC 9 cut(s) 123, 134, 428, 452, 689, 716, 793, 1050, 1315
HapII CCGG 9 cut(s) 24, 120, 179, 196, 208, 491, 575, 710, 790
HhaI GCGC 2 cut(s) 23, 580
Hin1I GRCGYC 2 cut(s) 764, 836
Hin1II CATG 5 cut(s) 722, 991, 1059, 1144, 1413
Hin6I GCGC 2 cut(s) 21, 578
HinP1I GCGC 2 cut(s) 21, 578
HindIII AAGCTT 1 cut(s) 1449
HpaII CCGG 9 cut(s) 24, 120, 179, 196, 208, 491, 575, 710, 790
HphI GGTGA 2 cut(s) 43, 601
Hpy188III TCNNGA 9 cut(s) 60, 84, 368, 437, 464, 485, 625, 954, 988
HpyAV CCTTC 9 cut(s) 241, 406, 580, 632, 667, 967, 988, 1254, 1323
HpyCH4III ACNGT 1 cut(s) 944
HpyCH4IV ACGT 3 cut(s) 111, 764, 836
HpyCH4V TGCA 5 cut(s) 291, 300, 595, 703, 1231
HpyF10VI GCNNNNNNNGC 3 cut(s) 297, 700, 706
HpyF3I CTNAG 8 cut(s) 356, 366, 922, 952, 1044, 1161, 1190, 1265
HpySE526I ACGT 3 cut(s) 111, 764, 836
Hsp92I GRCGYC 2 cut(s) 764, 836
Hsp92II CATG 5 cut(s) 722, 991, 1059, 1144, 1413
HspAI GCGC 2 cut(s) 21, 578
KroI GCCGGC 2 cut(s) 574, 789
KroNI GCCGGC 2 cut(s) 576, 791
Ksp22I TGATCA 1 cut(s) 1168
Kzo9I GATC 7 cut(s) 39, 62, 204, 433, 481, 784, 1168
LguI GCTCTTC 1 cut(s) 560
LmnI GCTCC 5 cut(s) 26, 62, 1134, 1273, 1433
Lsp1109I GCAGC 6 cut(s) 303, 606, 687, 927, 930, 1039
LweI GCATC 2 cut(s) 582, 1351
MaeI CTAG 1 cut(s) 932
MaeII ACGT 3 cut(s) 111, 764, 836
MaeIII GTNAC 1 cut(s) 31
MalI GATC 7 cut(s) 41, 64, 206, 435, 483, 786, 1170
MbiI CCGCTC 1 cut(s) 29
MboI GATC 7 cut(s) 39, 62, 204, 433, 481, 784, 1168
MflI RGATCY 1 cut(s) 204
MhlI GDGCHC 3 cut(s) 501, 1109, 1430
MluCI AATT 2 cut(s) 218, 274
MlyI GAGTC 4 cut(s) 28, 425, 731, 883
MmeI TCCRAC 5 cut(s) 444, 531, 729, 747, 1370
MroNI GCCGGC 2 cut(s) 574, 789
MroXI GAANNNNTTC 1 cut(s) 823
MseI TTAA 5 cut(s) 381, 528, 938, 1082, 1395
MslI CAYNNNNRTG 1 cut(s) 1139
MspA1I CMGCKG 1 cut(s) 1030
MspI CCGG 9 cut(s) 24, 120, 179, 196, 208, 491, 575, 710, 790
MspR9I CCNGG 8 cut(s) 120, 179, 196, 209, 470, 491, 492, 696
MvaI CCWGG 2 cut(s) 470, 696
MvnI CGCG 2 cut(s) 486, 580
MwoI GCNNNNNNNGC 3 cut(s) 297, 700, 706
NaeI GCCGGC 2 cut(s) 576, 791
NciI CCSGG 6 cut(s) 120, 179, 196, 209, 491, 492
NcoI CCATGG 1 cut(s) 1055
NdeII GATC 7 cut(s) 39, 62, 204, 433, 481, 784, 1168
NgoMIV GCCGGC 2 cut(s) 574, 789
NlaIII CATG 5 cut(s) 722, 991, 1059, 1144, 1413
NlaIV GGNNCC 2 cut(s) 58, 154
NmeAIII GCCGAG 1 cut(s) 310
NmuCI GTSAC 1 cut(s) 31
NruI TCGCGA 1 cut(s) 486
NspI RCATGY 1 cut(s) 1144
NspV TTCGAA 3 cut(s) 278, 320, 827
PaeR7I CTCGAG 1 cut(s) 443
PagI TCATGA 1 cut(s) 987
PceI AGGCCT 2 cut(s) 452, 1315
PciSI GCTCTTC 1 cut(s) 560
PcsI WCGNNNNNNNCGW 7 cut(s) 483, 731, 734, 743, 803, 833, 883
PdiI GCCGGC 2 cut(s) 576, 791
PdmI GAANNNNTTC 1 cut(s) 823
PfeI GAWTC 7 cut(s) 97, 268, 317, 352, 460, 628, 772
PflFI GACNNNGTC 1 cut(s) 8
PfoI TCCNGGA 1 cut(s) 207
PkrI GCNGC 7 cut(s) 293, 314, 621, 702, 917, 920, 1029
Ple19I CGATCG 1 cut(s) 484
PleI GAGTC 4 cut(s) 28, 424, 730, 882
PpsI GAGTC 4 cut(s) 28, 424, 730, 882
Psp124BI GAGCTC 1 cut(s) 1430
Psp6I CCWGG 2 cut(s) 468, 694
PspGI CCWGG 2 cut(s) 468, 694
PspN4I GGNNCC 2 cut(s) 58, 154
PspPI GGNCC 6 cut(s) 122, 426, 466, 687, 715, 1048
PspXI VCTCGAGB 1 cut(s) 443
PstI CTGCAG 1 cut(s) 1233
PsuI RGATCY 1 cut(s) 204
PsyI GACNNNGTC 1 cut(s) 8
PvuI CGATCG 1 cut(s) 484
RruI TCGCGA 1 cut(s) 486
RsaI GTAC 2 cut(s) 523, 999
RsaNI GTAC 2 cut(s) 522, 998
RseI CAYNNNNRTG 1 cut(s) 1139
SacI GAGCTC 1 cut(s) 1430
SapI GCTCTTC 1 cut(s) 560
SaqAI TTAA 5 cut(s) 381, 528, 938, 1082, 1395
SatI GCNGC 7 cut(s) 292, 313, 620, 701, 916, 919, 1028
Sau3AI GATC 7 cut(s) 39, 62, 204, 433, 481, 784, 1168
Sau96I GGNCC 6 cut(s) 122, 426, 466, 687, 715, 1048
SchI GAGTC 4 cut(s) 28, 425, 731, 883
ScrFI CCNGG 8 cut(s) 120, 179, 196, 209, 470, 491, 492, 696
SduI GDGCHC 3 cut(s) 501, 1109, 1430
SfaNI GCATC 2 cut(s) 582, 1351
SfcI CTRYAG 1 cut(s) 1229
Sfr274I CTCGAG 1 cut(s) 443
SfuI TTCGAA 3 cut(s) 278, 320, 827
SinI GGWCC 1 cut(s) 466
SlaI CTCGAG 1 cut(s) 443
SmaI CCCGGG 1 cut(s) 492
SmiMI CAYNNNNRTG 1 cut(s) 1139
SmlI CTYRAG 1 cut(s) 443
SmoI CTYRAG 1 cut(s) 443
Sse9I AATT 2 cut(s) 218, 274
SseBI AGGCCT 2 cut(s) 452, 1315
SsiI CCGC 5 cut(s) 29, 312, 707, 1030, 1144
SspMI CTAG 1 cut(s) 932
SstI GAGCTC 1 cut(s) 1430
StuI AGGCCT 2 cut(s) 452, 1315
StyD4I CCNGG 8 cut(s) 118, 177, 194, 207, 468, 489, 490, 694
StyI CCWWGG 2 cut(s) 633, 1055
TaaI ACNGT 1 cut(s) 944
TaiI ACGT 3 cut(s) 114, 767, 839
TaqI TCGA 7 cut(s) 278, 320, 410, 444, 477, 827, 839
TasI AATT 2 cut(s) 218, 274
TauI GCSGC 1 cut(s) 315
TfiI GAWTC 7 cut(s) 97, 268, 317, 352, 460, 628, 772
Tru1I TTAA 5 cut(s) 381, 528, 938, 1082, 1395
Tru9I TTAA 5 cut(s) 381, 528, 938, 1082, 1395
TscAI CASTG 1 cut(s) 1141
TseFI GTSAC 1 cut(s) 31
TseI GCWGC 6 cut(s) 291, 619, 700, 915, 918, 1027
Tsp45I GTSAC 1 cut(s) 31
TspDTI ATGAA 6 cut(s) 976, 986, 1004, 1083, 1309, 1352
TspMI CCCGGG 1 cut(s) 490
TspRI CASTG 1 cut(s) 1141
Tth111I GACNNNGTC 1 cut(s) 8
VpaK11BI GGWCC 1 cut(s) 466
XapI RAATTY 2 cut(s) 218, 274
XceI RCATGY 1 cut(s) 1144
XhoI CTCGAG 1 cut(s) 443
XmaI CCCGGG 1 cut(s) 490
XmnI GAANNNNTTC 1 cut(s) 823
XspI CTAG 1 cut(s) 932
ZraI GACGTC 2 cut(s) 765, 837
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.