MD17G1057000.v1.1

Targeting protein for Xklp2 (TPX2)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0004726
Physical Location & Seq
Forward (+)
4642585 .. 4645279
2695 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1057000.v1.1.491

Sequence Viewer

Length: 1503 bp
ATGGATTTTATCGGCAAGAACGCCGGAGTGGTGACTCCTGTGAAAGACCAACACGGGTCTCGATACAAGAAGCACGAGAAATCAAGATGCACCGAGAATATGAACCCCAATGTTTCGCCTGGCCCGAAACACACTGCTTCCCCTGCGACGAAATCGGCGGGGAAGTCCCAGAAATCGGCGTCCAAGAACCCGAACCCGAACCCGGTTGTTCGTTCGCCCCGGAACAAGATCCGGGAGAGGAAGTTCATCGTTGCGAAGAAGAAATCAAAGAAGGAGAACCCGAACGTCTCATGCAAGTGCAAAGATAAGGGCAATTCGAAGATGTGCCTCTGCGTTGCGTATGAGAATCTGAGGGCGTCACAGGAAGAGTTCTTCAAGAAGCGAAACGACGACGTCGAATCGGAGAGTTTGAAGGAGAGCGAAAGAGCTGGGCGTGAACTGGAGGAAGAAATCGAGGAGGGTTTGAGGATTCAGGACCTTCAGATTGAAAATGGGTCGGGTGATATTGACCTGGATAGTGAAATGGGTTGTTCAACAATCAAGAGAAGAAGGGACAAGTTGCTGGAAGAGGCGAGGCAGAGCGTGCCGAAGAGTGGGAAGGTGATGCATTTGGTTCAGGCGTTTGAGAAGCTGCTTTCGATTCCGAGCTCGAAGGATTCGGATGAGCAGAATGGGGAGGAGGAAGCGGCGGAAGAGATTGGGAAGAAGGCGGCGAAGTGGGCATTGCCCGGATTGCAGCCTCCTCCTAAGGCGCTAGAAACACAGGTCTCGTCTTCTTCGTTTTTACCGTCGGACTTGTTTTTGACGTCGGAGAATCTCGGTCTGGATCGGCGAACTTCAGTCTCTTCTTCTTGGGATGGAAGTCTGGGAAGTGTCTCAAGCAGGACTTCGAATGGGGAGCGAAGGAGCCGAAGAAATAGCTCTGAATCTTCCAGCACAATCGGAGGAGGGAGATGGAAGAAGAAGAAGCAACAGAGAGCCACTAGCGCTAAGCCATTCAAGCTAAGAACAGAGGAAAGGGGAAGACAAAAGGAGGAAGAGTTCACGAAGAAGCTACAAGAGATGATGATGGAGGAGGAGAGACAGCGGATACCAATTGCTCAGGGCCTCCCATGGACAACTGACGAACCAGAGTGCTTACTGAAACCTCCAGTAAAAGAAATAACAATACCAACAGACCTGAAGCTCCACAGTGACATGCGGGCAGTAGAGCGAGCTGAGTTTGACCATCAGGTGGCAGAGAAGATGAGCCTGTTTGAGCAATACAAGATGGAAAGAGAAAGACTGCTGAAGTTGGCAGAAGAGGAGGAAATAAAAAGATTGAAGAGGGAGCTTGTTCCGAAAGCGCAGCCAATGCCCTACTTTGACAGGCCTTTCATTCCCAGAAGGTCAATGAAGCATCCAACCATACCGAAAGAACCGAAGTTTCATGTGCCTCAAAACAAGAAGATCAAATGCTACTTGTCGTGGGACGAGATGAGCACCTACACTTACGAACATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPX2

Protein Analysis

501

Amino Acids

57.15

Weight (kDa)

9.29

Isoelectric Point (pI)

67.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPX2 PF06886 395 - 469 4.3e-23 Targeting protein for Xklp2 (TPX2) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 396, 809
AccB7I CCANNNNNTGG 1 cut(s) 1234
AciI CCGC 6 cut(s) 158, 686, 689, 710, 1087, 1201
AclWI GGATC 2 cut(s) 223, 834
AcuI CTGAAG 4 cut(s) 464, 822, 1202, 1310
AcyI GRCGYC 4 cut(s) 179, 356, 393, 806
AfeI AGCGCT 1 cut(s) 988
AfiI CCNNNNNNNGG 4 cut(s) 175, 202, 593, 1234
AgsI TTSAA 6 cut(s) 376, 412, 488, 534, 1000, 1324
AjnI CCWGG 2 cut(s) 118, 510
AloI GAACNNNNNNTCC 4 cut(s) 227, 259, 1025, 1057
AluBI AGCT 9 cut(s) 428, 631, 648, 921, 1003, 1054, 1186, 1217, 1333
AluI AGCT 9 cut(s) 428, 631, 648, 921, 1003, 1054, 1186, 1217, 1333
Alw21I GWGCWC 2 cut(s) 650, 1484
Alw26I GTCTC 6 cut(s) 63, 292, 772, 847, 880, 1075
AlwI GGATC 2 cut(s) 223, 834
Aor51HI AGCGCT 1 cut(s) 988
AoxI GGCC 3 cut(s) 121, 1105, 1370
ApeKI GCWGC 3 cut(s) 631, 736, 1348
AspLEI GCGC 3 cut(s) 754, 989, 1348
AspS9I GGNCC 3 cut(s) 122, 475, 1105
AsuC2I CCSGG 4 cut(s) 203, 220, 233, 729
AsuHPI GGTGA 3 cut(s) 43, 512, 613
AsuII TTCGAA 2 cut(s) 317, 890
AvaII GGWCC 1 cut(s) 475
AxyI CCTNAGG 1 cut(s) 747
BanII GRGCYC 1 cut(s) 650
BauI CACGAG 1 cut(s) 74
BbsI GAAGAC 2 cut(s) 765, 1030
Bbv12I GWGCWC 2 cut(s) 650, 1484
BbvI GCAGC 3 cut(s) 618, 748, 1360
BccI CCATC 5 cut(s) 851, 948, 1063, 1236, 1264
BciT130I CCWGG 2 cut(s) 120, 512
BciVI GTATCC 1 cut(s) 1083
BcnI CCSGG 4 cut(s) 203, 220, 233, 729
BcoDI GTCTC 6 cut(s) 63, 292, 772, 847, 880, 1075
BfaI CTAG 2 cut(s) 755, 984
BfoI RGCGCY 2 cut(s) 755, 990
BfuI GTATCC 1 cut(s) 1083
BisI GCNGC 5 cut(s) 632, 687, 711, 737, 1349
BlpI GCTNAGC 1 cut(s) 990
BlsI GCNGC 5 cut(s) 633, 688, 712, 738, 1350
Bme1390I CCNGG 6 cut(s) 120, 203, 220, 233, 512, 729
Bme18I GGWCC 1 cut(s) 475
BmgT120I GGNCC 3 cut(s) 122, 475, 1105
BmiI GGNNCC 1 cut(s) 908
BmrFI CCNGG 6 cut(s) 120, 203, 220, 233, 512, 729
BmsI GCATC 3 cut(s) 77, 594, 1408
BpiI GAAGAC 2 cut(s) 765, 1030
BpmI CTGGAG 2 cut(s) 461, 1134
Bpu10I CCTNAGC 1 cut(s) 1101
Bpu1102I GCTNAGC 1 cut(s) 990
Bpu14I TTCGAA 2 cut(s) 317, 890
BpuEI CTTGAG 1 cut(s) 862
BpuMI CCSGG 4 cut(s) 203, 220, 233, 729
BsaHI GRCGYC 4 cut(s) 179, 356, 393, 806
BsaI GGTCTC 2 cut(s) 63, 772
BsaJI CCNNGG 2 cut(s) 218, 1112
BsaXI ACNNNNNCTCC 4 cut(s) 227, 257, 1025, 1055
Bsc4I CCNNNNNNNGG 4 cut(s) 175, 202, 593, 1234
Bse1I ACTGG 2 cut(s) 444, 1151
Bse21I CCTNAGG 1 cut(s) 747
Bse3DI GCAATG 1 cut(s) 722
BseBI CCWGG 2 cut(s) 120, 512
BseDI CCNNGG 2 cut(s) 218, 1112
BseGI GGATG 3 cut(s) 667, 862, 1399
BseLI CCNNNNNNNGG 4 cut(s) 175, 202, 593, 1234
BseMI GCAATG 1 cut(s) 722
BseMII CTCAG 3 cut(s) 341, 1115, 1209
BseNI ACTGG 2 cut(s) 444, 1151
BseRI GAGGAG 7 cut(s) 470, 692, 732, 960, 1088, 1091, 1319
BseXI GCAGC 3 cut(s) 618, 748, 1360
BseYI CCCAGC 1 cut(s) 428
BshFI GGCC 3 cut(s) 123, 1107, 1372
BsiHKAI GWGCWC 2 cut(s) 650, 1484
BsiSI CCGG 5 cut(s) 24, 203, 220, 232, 729
BslFI GGGAC 3 cut(s) 151, 566, 1484
BslI CCNNNNNNNGG 4 cut(s) 175, 202, 593, 1234
BsmAI GTCTC 6 cut(s) 63, 292, 772, 847, 880, 1075
BsmBI CGTCTC 1 cut(s) 292
BsmFI GGGAC 3 cut(s) 151, 566, 1484
BsnI GGCC 3 cut(s) 123, 1107, 1372
Bso31I GGTCTC 2 cut(s) 63, 772
Bsp119I TTCGAA 2 cut(s) 317, 890
Bsp1286I GDGCHC 2 cut(s) 650, 1484
Bsp143I GATC 3 cut(s) 228, 826, 1449
Bsp1720I GCTNAGC 1 cut(s) 990
Bsp19I CCATGG 1 cut(s) 1112
BspACI CCGC 6 cut(s) 158, 686, 689, 710, 1087, 1201
BspANI GGCC 3 cut(s) 123, 1107, 1372
BspCNI CTCAG 3 cut(s) 342, 1114, 1210
BspLI GGNNCC 1 cut(s) 908
BspPI GGATC 2 cut(s) 223, 834
BspT104I TTCGAA 2 cut(s) 317, 890
BspTNI GGTCTC 2 cut(s) 63, 772
BsrDI GCAATG 1 cut(s) 722
BsrI ACTGG 2 cut(s) 444, 1151
BssECI CCNNGG 2 cut(s) 218, 1112
BssMI GATC 3 cut(s) 228, 826, 1449
BssNI GRCGYC 4 cut(s) 179, 356, 393, 806
BssSI CACGAG 1 cut(s) 74
BssT1I CCWWGG 1 cut(s) 1112
Bst2BI CACGAG 1 cut(s) 74
Bst2UI CCWGG 2 cut(s) 120, 512
Bst4CI ACNGT 2 cut(s) 789, 1193
Bst6I CTCTTC 8 cut(s) 360, 561, 584, 687, 850, 1032, 1296, 1319
BstACI GRCGYC 4 cut(s) 179, 356, 393, 806
BstAPI GCANNNNNTGC 2 cut(s) 583, 1354
BstBI TTCGAA 2 cut(s) 317, 890
BstC8I GCNNGC 3 cut(s) 584, 1203, 1215
BstDEI CTNAG 6 cut(s) 350, 747, 990, 1004, 1101, 1218
BstDSI CCRYGG 1 cut(s) 1112
BstF5I GGATG 3 cut(s) 667, 862, 1399
BstH2I RGCGCY 2 cut(s) 755, 990
BstHHI GCGC 3 cut(s) 754, 989, 1348
BstKTI GATC 3 cut(s) 231, 829, 1452
BstMAI GTCTC 6 cut(s) 63, 292, 772, 847, 880, 1075
BstMBI GATC 3 cut(s) 228, 826, 1449
BstMWI GCNNNNNNNGC 7 cut(s) 143, 583, 719, 733, 986, 1000, 1354
BstNI CCWGG 2 cut(s) 120, 512
BstNSI RCATGY 1 cut(s) 1201
BstSCI CCNGG 6 cut(s) 118, 201, 218, 231, 510, 727
BstV1I GCAGC 3 cut(s) 618, 748, 1360
BstV2I GAAGAC 2 cut(s) 765, 1030
BstX2I RGATCY 1 cut(s) 228
BstYI RGATCY 1 cut(s) 228
Bsu36I CCTNAGG 1 cut(s) 747
BsuI GTATCC 1 cut(s) 1083
BsuRI GGCC 3 cut(s) 123, 1107, 1372
BtgI CCRYGG 1 cut(s) 1112
BtsCI GGATG 3 cut(s) 667, 862, 1399
BtsI GCAGTG 1 cut(s) 132
BtsIMutI CAGTG 2 cut(s) 132, 1198
Cac8I GCNNGC 3 cut(s) 584, 1203, 1215
CfoI GCGC 3 cut(s) 754, 989, 1348
Cfr13I GGNCC 3 cut(s) 122, 475, 1105
CseI GACGC 2 cut(s) 168, 345
CviAII CATG 4 cut(s) 291, 1113, 1198, 1430
DdeI CTNAG 6 cut(s) 350, 747, 990, 1004, 1101, 1218
DpnI GATC 3 cut(s) 230, 828, 1451
DpnII GATC 3 cut(s) 228, 826, 1449
Eam1104I CTCTTC 8 cut(s) 360, 561, 584, 687, 850, 1032, 1296, 1319
EarI CTCTTC 8 cut(s) 360, 561, 584, 687, 850, 1032, 1296, 1319
EciI GGCGGA 1 cut(s) 704
Ecl136II GAGCTC 1 cut(s) 648
Eco130I CCWWGG 1 cut(s) 1112
Eco147I AGGCCT 1 cut(s) 1372
Eco24I GRGCYC 1 cut(s) 650
Eco31I GGTCTC 2 cut(s) 63, 772
Eco47I GGWCC 1 cut(s) 475
Eco47III AGCGCT 1 cut(s) 988
Eco53kI GAGCTC 1 cut(s) 648
Eco57I CTGAAG 4 cut(s) 464, 822, 1202, 1310
Eco81I CCTNAGG 1 cut(s) 747
EcoICRI GAGCTC 1 cut(s) 648
EcoO109I RGGNCCY 2 cut(s) 475, 1105
EcoRII CCWGG 2 cut(s) 118, 510
EcoT14I CCWWGG 1 cut(s) 1112
EcoT22I ATGCAT 1 cut(s) 609
EcoT38I GRGCYC 1 cut(s) 650
ErhI CCWWGG 1 cut(s) 1112
Esp3I CGTCTC 1 cut(s) 292
FaeI CATG 4 cut(s) 294, 1116, 1201, 1433
FaiI YATR 7 cut(s) 101, 292, 342, 1114, 1199, 1409, 1431
FalI AAGNNNNNCTT 2 cut(s) 871, 903
FaqI GGGAC 3 cut(s) 151, 566, 1484
FatI CATG 4 cut(s) 290, 1112, 1197, 1429
FauI CCCGC 2 cut(s) 151, 1194
Fnu4HI GCNGC 5 cut(s) 632, 687, 711, 737, 1349
FokI GGATG 3 cut(s) 674, 869, 1386
FriOI GRGCYC 1 cut(s) 650
Fsp4HI GCNGC 5 cut(s) 632, 687, 711, 737, 1349
FspBI CTAG 2 cut(s) 755, 984
GlaI GCGC 3 cut(s) 753, 988, 1347
GluI GCNGC 5 cut(s) 632, 687, 711, 737, 1349
GsaI CCCAGC 1 cut(s) 432
GsuI CTGGAG 2 cut(s) 461, 1134
HaeII RGCGCY 2 cut(s) 755, 990
HaeIII GGCC 3 cut(s) 123, 1107, 1372
HapII CCGG 5 cut(s) 24, 203, 220, 232, 729
HgaI GACGC 2 cut(s) 168, 345
HhaI GCGC 3 cut(s) 754, 989, 1348
Hin1I GRCGYC 4 cut(s) 179, 356, 393, 806
Hin1II CATG 4 cut(s) 294, 1116, 1201, 1433
Hin6I GCGC 3 cut(s) 752, 987, 1346
HinP1I GCGC 3 cut(s) 752, 987, 1346
HinfI GANTC 8 cut(s) 34, 346, 398, 469, 640, 656, 814, 926
HpaII CCGG 5 cut(s) 24, 203, 220, 232, 729
HphI GGTGA 3 cut(s) 43, 512, 613
Hpy166II GTNNAC 2 cut(s) 437, 1044
Hpy188III TCNNGA 7 cut(s) 60, 84, 376, 473, 541, 824, 1045
Hpy8I GTNNAC 2 cut(s) 437, 1044
Hpy99I CGWCG 6 cut(s) 151, 392, 395, 398, 793, 811
HpyAV CCTTC 9 cut(s) 265, 406, 488, 543, 592, 646, 700, 897, 1380
HpyCH4III ACNGT 2 cut(s) 789, 1193
HpyCH4IV ACGT 3 cut(s) 285, 393, 806
HpyCH4V TGCA 5 cut(s) 90, 294, 300, 607, 736
HpyF10VI GCNNNNNNNGC 7 cut(s) 143, 583, 719, 733, 986, 1000, 1354
HpyF3I CTNAG 6 cut(s) 350, 747, 990, 1004, 1101, 1218
HpySE526I ACGT 3 cut(s) 285, 393, 806
Hsp92I GRCGYC 4 cut(s) 179, 356, 393, 806
Hsp92II CATG 4 cut(s) 294, 1116, 1201, 1433
HspAI GCGC 3 cut(s) 752, 987, 1346
Kzo9I GATC 3 cut(s) 228, 826, 1449
LmnI GCTCC 4 cut(s) 898, 906, 1191, 1330
Lsp1109I GCAGC 3 cut(s) 618, 748, 1360
LweI GCATC 3 cut(s) 77, 594, 1408
MaeI CTAG 2 cut(s) 755, 984
MaeII ACGT 3 cut(s) 285, 393, 806
MaeIII GTNAC 3 cut(s) 31, 357, 1193
MalI GATC 3 cut(s) 230, 828, 1451
MboI GATC 3 cut(s) 228, 826, 1449
MfeI CAATTG 1 cut(s) 1095
MflI RGATCY 1 cut(s) 228
MhlI GDGCHC 2 cut(s) 650, 1484
MluCI AATT 2 cut(s) 313, 1095
MlyI GAGTC 1 cut(s) 28
MmeI TCCRAC 3 cut(s) 771, 789, 1427
Mph1103I ATGCAT 1 cut(s) 609
MslI CAYNNNNRTG 1 cut(s) 295
MspA1I CMGCKG 1 cut(s) 1087
MspI CCGG 5 cut(s) 24, 203, 220, 232, 729
MspR9I CCNGG 6 cut(s) 120, 203, 220, 233, 512, 729
MunI CAATTG 1 cut(s) 1095
MvaI CCWGG 2 cut(s) 120, 512
MwoI GCNNNNNNNGC 7 cut(s) 143, 583, 719, 733, 986, 1000, 1354
NciI CCSGG 4 cut(s) 203, 220, 233, 729
NcoI CCATGG 1 cut(s) 1112
NdeII GATC 3 cut(s) 228, 826, 1449
NlaIII CATG 4 cut(s) 294, 1116, 1201, 1433
NlaIV GGNNCC 1 cut(s) 908
NmuCI GTSAC 3 cut(s) 31, 357, 1193
NsiI ATGCAT 1 cut(s) 609
NspI RCATGY 1 cut(s) 1201
NspV TTCGAA 2 cut(s) 317, 890
PceI AGGCCT 1 cut(s) 1372
PcsI WCGNNNNNNNCGW 4 cut(s) 122, 393, 776, 785
PfeI GAWTC 7 cut(s) 346, 398, 469, 640, 656, 814, 926
PflFI GACNNNGTC 1 cut(s) 392
PflMI CCANNNNNTGG 1 cut(s) 1234
PfoI TCCNGGA 1 cut(s) 231
PkrI GCNGC 5 cut(s) 633, 688, 712, 738, 1350
PleI GAGTC 1 cut(s) 28
PpsI GAGTC 1 cut(s) 28
PpuMI RGGWCCY 1 cut(s) 475
Psp124BI GAGCTC 1 cut(s) 650
Psp5II RGGWCCY 1 cut(s) 475
Psp6I CCWGG 2 cut(s) 118, 510
PspFI CCCAGC 1 cut(s) 428
PspGI CCWGG 2 cut(s) 118, 510
PspN4I GGNNCC 1 cut(s) 908
PspPI GGNCC 3 cut(s) 122, 475, 1105
PspPPI RGGWCCY 1 cut(s) 475
PsuI RGATCY 1 cut(s) 228
PsyI GACNNNGTC 1 cut(s) 392
RseI CAYNNNNRTG 1 cut(s) 295
SacI GAGCTC 1 cut(s) 650
SatI GCNGC 5 cut(s) 632, 687, 711, 737, 1349
Sau3AI GATC 3 cut(s) 228, 826, 1449
Sau96I GGNCC 3 cut(s) 122, 475, 1105
SchI GAGTC 1 cut(s) 28
ScrFI CCNGG 6 cut(s) 120, 203, 220, 233, 512, 729
SduI GDGCHC 2 cut(s) 650, 1484
SfaNI GCATC 3 cut(s) 77, 594, 1408
SfuI TTCGAA 2 cut(s) 317, 890
SinI GGWCC 1 cut(s) 475
SmiMI CAYNNNNRTG 1 cut(s) 295
SmlI CTYRAG 1 cut(s) 877
SmoI CTYRAG 1 cut(s) 877
Sse9I AATT 2 cut(s) 313, 1095
SseBI AGGCCT 1 cut(s) 1372
SsiI CCGC 6 cut(s) 158, 686, 689, 710, 1087, 1201
SspMI CTAG 2 cut(s) 755, 984
SstI GAGCTC 1 cut(s) 650
StuI AGGCCT 1 cut(s) 1372
StyD4I CCNGG 6 cut(s) 118, 201, 218, 231, 510, 727
StyI CCWWGG 1 cut(s) 1112
TaaI ACNGT 2 cut(s) 789, 1193
TaiI ACGT 3 cut(s) 288, 396, 809
TaqI TCGA 7 cut(s) 61, 317, 396, 453, 638, 650, 890
TaqII GACCGA 1 cut(s) 809
TasI AATT 2 cut(s) 313, 1095
TauI GCSGC 2 cut(s) 689, 713
TfiI GAWTC 7 cut(s) 346, 398, 469, 640, 656, 814, 926
TscAI CASTG 2 cut(s) 139, 1198
TseFI GTSAC 3 cut(s) 31, 357, 1193
TseI GCWGC 3 cut(s) 631, 736, 1348
Tsp45I GTSAC 3 cut(s) 31, 357, 1193
TspDTI ATGAA 5 cut(s) 116, 235, 1366, 1409, 1418
TspRI CASTG 2 cut(s) 139, 1198
Tth111I GACNNNGTC 1 cut(s) 392
Van91I CCANNNNNTGG 1 cut(s) 1234
VpaK11BI GGWCC 1 cut(s) 475
XceI RCATGY 1 cut(s) 1201
XspI CTAG 2 cut(s) 755, 984
ZraI GACGTC 2 cut(s) 394, 807
Zsp2I ATGCAT 1 cut(s) 609
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.