Rmu_sc0020961.1_g000001

Targeting protein for Xklp2 (TPX2)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0020961.1
OG0004726
Physical Location & Seq
Reverse (-)
2 .. 277
276 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0020961.1_g000001.1.cds

Sequence Viewer

Length: 276 bp
atggactcggtcggcaagagcgccggagcagtgactccgatcagagaccaacacggctcccgatccaagaagcaagaaaactccagatactccgagaatctgaaccccaacgtctctcccggcccgaaatcggccaagtcccagaaatcgggttccaagaacccgaacaacccgaacccggttttccagtcgcccaggaagaagatccgggagaggaaattcgtggtggcgaagaagagagggaagaaggaggagaatccgaatccgaatccgaat
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPX2

Protein Analysis

92

Amino Acids

10.16

Weight (kDa)

10.73

Isoelectric Point (pI)

41.89

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 57, 199
AcoI YGGCCR 1 cut(s) 132
AcsI RAATTY 1 cut(s) 218
AfiI CCNNNNNNNGG 3 cut(s) 130, 148, 178
AjnI CCWGG 1 cut(s) 194
Alw26I GTCTC 2 cut(s) 39, 118
AlwI GGATC 2 cut(s) 57, 199
AoxI GGCC 2 cut(s) 121, 132
ApoI RAATTY 1 cut(s) 218
AspLEI GCGC 1 cut(s) 23
AspS9I GGNCC 1 cut(s) 122
AsuC2I CCSGG 3 cut(s) 120, 179, 209
BceAI ACGGC 1 cut(s) 70
BciT130I CCWGG 1 cut(s) 196
BcnI CCSGG 3 cut(s) 120, 179, 209
BcoDI GTCTC 2 cut(s) 39, 118
BfoI RGCGCY 1 cut(s) 24
Bme1390I CCNGG 4 cut(s) 120, 179, 196, 209
BmgT120I GGNCC 1 cut(s) 122
BmiI GGNNCC 2 cut(s) 58, 154
BmrFI CCNGG 4 cut(s) 120, 179, 196, 209
BpmI CTGGAG 1 cut(s) 67
BpuMI CCSGG 3 cut(s) 120, 179, 209
BsaI GGTCTC 1 cut(s) 39
BsaJI CCNNGG 1 cut(s) 194
Bsc4I CCNNNNNNNGG 3 cut(s) 130, 148, 178
Bse1I ACTGG 1 cut(s) 187
BseBI CCWGG 1 cut(s) 196
BseDI CCNNGG 1 cut(s) 194
BseLI CCNNNNNNNGG 3 cut(s) 130, 148, 178
BseNI ACTGG 1 cut(s) 187
BseRI GAGGAG 1 cut(s) 266
Bsh1285I CGRYCG 1 cut(s) 12
BshFI GGCC 2 cut(s) 123, 134
BsiEI CGRYCG 1 cut(s) 12
BsiSI CCGG 4 cut(s) 24, 120, 179, 208
BslFI GGGAC 1 cut(s) 124
BslI CCNNNNNNNGG 3 cut(s) 130, 148, 178
BsmAI GTCTC 2 cut(s) 39, 118
BsmBI CGTCTC 1 cut(s) 118
BsmFI GGGAC 1 cut(s) 124
BsnI GGCC 2 cut(s) 123, 134
Bso31I GGTCTC 1 cut(s) 39
Bsp143I GATC 3 cut(s) 39, 62, 204
BspANI GGCC 2 cut(s) 123, 134
BspLI GGNNCC 2 cut(s) 58, 154
BspPI GGATC 2 cut(s) 57, 199
BspTNI GGTCTC 1 cut(s) 39
BsrI ACTGG 1 cut(s) 187
BssECI CCNNGG 1 cut(s) 194
BssMI GATC 3 cut(s) 39, 62, 204
Bst2UI CCWGG 1 cut(s) 196
Bst6I CTCTTC 1 cut(s) 230
BstH2I RGCGCY 1 cut(s) 24
BstHHI GCGC 1 cut(s) 23
BstKTI GATC 3 cut(s) 42, 65, 207
BstMAI GTCTC 2 cut(s) 39, 118
BstMBI GATC 3 cut(s) 39, 62, 204
BstMCI CGRYCG 1 cut(s) 12
BstNI CCWGG 1 cut(s) 196
BstSCI CCNGG 4 cut(s) 118, 177, 194, 207
BstX2I RGATCY 1 cut(s) 204
BstYI RGATCY 1 cut(s) 204
BsuRI GGCC 2 cut(s) 123, 134
BtsI GCAGTG 1 cut(s) 36
BtsIMutI CAGTG 1 cut(s) 36
CfoI GCGC 1 cut(s) 23
Cfr13I GGNCC 1 cut(s) 122
CviJI RGCY 3 cut(s) 57, 123, 134
CviKI_1 RGCY 3 cut(s) 57, 123, 134
DpnI GATC 3 cut(s) 41, 64, 206
DpnII GATC 3 cut(s) 39, 62, 204
EaeI YGGCCR 1 cut(s) 132
Eam1104I CTCTTC 1 cut(s) 230
EarI CTCTTC 1 cut(s) 230
Eco31I GGTCTC 1 cut(s) 39
EcoRII CCWGG 1 cut(s) 194
Esp3I CGTCTC 1 cut(s) 118
FaqI GGGAC 1 cut(s) 124
GlaI GCGC 1 cut(s) 22
GsuI CTGGAG 1 cut(s) 67
HaeII RGCGCY 1 cut(s) 24
HaeIII GGCC 2 cut(s) 123, 134
HapII CCGG 4 cut(s) 24, 120, 179, 208
HhaI GCGC 1 cut(s) 23
Hin6I GCGC 1 cut(s) 21
HinP1I GCGC 1 cut(s) 21
HinfI GANTC 6 cut(s) 5, 34, 97, 256, 262, 268
HpaII CCGG 4 cut(s) 24, 120, 179, 208
Hpy188I TCNGA 7 cut(s) 39, 44, 94, 102, 261, 267, 273
Hpy188III TCNNGA 2 cut(s) 60, 84
HpyAV CCTTC 1 cut(s) 241
HpyCH4IV ACGT 1 cut(s) 111
HpySE526I ACGT 1 cut(s) 111
HspAI GCGC 1 cut(s) 21
Kzo9I GATC 3 cut(s) 39, 62, 204
LmnI GCTCC 2 cut(s) 26, 62
LpnPI CCDG 9 cut(s) 37, 97, 133, 155, 181, 192, 200, 208, 221
MaeII ACGT 1 cut(s) 111
MaeIII GTNAC 1 cut(s) 31
MalI GATC 3 cut(s) 41, 64, 206
MboI GATC 3 cut(s) 39, 62, 204
MboII GAAGA 5 cut(s) 211, 214, 244, 247, 256
MflI RGATCY 1 cut(s) 204
MluCI AATT 1 cut(s) 218
MlyI GAGTC 1 cut(s) 28
MnlI CCTC 3 cut(s) 207, 233, 244
MspI CCGG 4 cut(s) 24, 120, 179, 208
MspR9I CCNGG 4 cut(s) 120, 179, 196, 209
MvaI CCWGG 1 cut(s) 196
NciI CCSGG 3 cut(s) 120, 179, 209
NdeII GATC 3 cut(s) 39, 62, 204
NlaIV GGNNCC 2 cut(s) 58, 154
NmuCI GTSAC 1 cut(s) 31
PfeI GAWTC 4 cut(s) 97, 256, 262, 268
PflFI GACNNNGTC 1 cut(s) 8
PfoI TCCNGGA 1 cut(s) 207
PleI GAGTC 1 cut(s) 28
PpsI GAGTC 1 cut(s) 28
Psp6I CCWGG 1 cut(s) 194
PspGI CCWGG 1 cut(s) 194
PspN4I GGNNCC 2 cut(s) 58, 154
PspPI GGNCC 1 cut(s) 122
PsuI RGATCY 1 cut(s) 204
PsyI GACNNNGTC 1 cut(s) 8
Sau3AI GATC 3 cut(s) 39, 62, 204
Sau96I GGNCC 1 cut(s) 122
SchI GAGTC 1 cut(s) 28
ScrFI CCNGG 4 cut(s) 120, 179, 196, 209
SetI ASST 1 cut(s) 114
Sse9I AATT 1 cut(s) 218
StyD4I CCNGG 4 cut(s) 118, 177, 194, 207
TaiI ACGT 1 cut(s) 114
TasI AATT 1 cut(s) 218
TfiI GAWTC 4 cut(s) 97, 256, 262, 268
TscAI CASTG 1 cut(s) 36
TseFI GTSAC 1 cut(s) 31
Tsp45I GTSAC 1 cut(s) 31
TspRI CASTG 1 cut(s) 36
Tth111I GACNNNGTC 1 cut(s) 8
XapI RAATTY 1 cut(s) 218
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.