MD09G1063100.v1.1

Targeting protein for Xklp2 (TPX2)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0004726
Physical Location & Seq
Forward (+)
4273428 .. 4276600
3173 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1063100.v1.1.491

Sequence Viewer

Length: 1494 bp
ATGGATTTGATCGGCAAGAACGCCGGAGCGGTGACTCCTGTGAAAAACCAACATGGGTCTCGATCCAAGAAGCACGACAACTCGAGATACACTGAGAATTCGAACCCCAATGTTTCCCCTGGCCCCAAACCCACTGCTTCCCCTGCGTCGAAATCGGCGGCGAAGTCCCAGAAATCGGCGTCCAAGAACACAAACCCGGTTGTTCGTTCGCCCCGGAACAAGATCCGGGAGAGAAAGTTCGTCGTCGCGAAGAAGAAATCGAAGAAGGAGAATCCGAATGTCGCCTGCAAGTGCAAAGAGAAGGGAAATTCGAAAATGTGCCTCTGCGTTGCGTATGAAAATCTACGGGCGTCGCAGGAAGAGTTCTTCAAGAAGCGAAACGACGACGTCGAATCCGAGAGTTTGAAGGAGAGCGACAGAGCTGAGCGTGAACTGGAGGAAGCAATCGAGGAGGGTTTGAGGATTCAGGACCTTCATATTGAAGATGGGTCGGGTGATTTGGACCCGGATAGTGAATTGGGTTGTTCGACAATCAAGAGAAGAAGGGACAAGTTGTTGGAAGAGGCGAGGAAGAGTGTGCCAGAGCGTGGGAAGGTGATGCATTTGATTCAGGCATTTGAGAAGCTGCTTTCGATTCCAAGCTCAAAGGATTCGGATGAGCAGAATGGGGAGGAGGAGGCAGCGGAAGAGATTGGGAAGAAGGTAGAGAAGTGGACATTGCCTGGATTGCAGCCTCCTCCGAATGCGCCGGAAACACAGGTTTCGTCGTCGTTTTTCCCGTCTGATTTGTGCTTGACTTCGGAGAATCTCGGTCTGGATCGGCGGCCTTCAGTCTCTTCTTCTTGGGATGGAAGTCTGGGAAGTGGTTCAAGCAGGACTTCTAACGGGGGGCGAAGAAACAGAAGAAATAGCTCTGAATCTTCTAGCACAATGGGAGGAACGAGATGGAAGAAGAAGCGGCAACAGAGAGCCACTAGCGCTAAGCCATTCAAGCTAAGAACAGAGGAAAGGGGAAGACAAAAGGAGGAAGAGTTCGTGAAGAAGTTACAAGAGATGATGATGGAGGAGGAGAGGCAGCGGATACCAATTGCTCAGGGCCTCCCATGGACAACAGACGAACCAGAGTGCTTGCTGAAACCTCCGGTAAAAGAAATTACAATACCAACCGACCTGAAGCTCCACAGTGACATGCGGGCAGTAGAGCGAGCTGAGTTTGATCATCAGGTGGCAGAGAAGATGACCCTGTTTGAGCAATACAAGATGGAAAGAGAGAGACTGCAGAAGTTGGCAGAAGAGGAGGAAATAAGAAGATTGAGAAAGGAGCTTGTCCCAAAAGCACAGCCGATGCCCTACTTTGACAGGCCTTTCATTCCCAGAAGGTCAATGAAGCATCCAACTATACCGAAAGAACCGAAGTTTCATGTGCCTCAAAACAAGAAGATCAAGTGCTGCTTGTCATGGAACGATATGAGCTCCTACACTTACGAACATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPX2

Protein Analysis

498

Amino Acids

56.75

Weight (kDa)

9.26

Isoelectric Point (pI)

68.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPX2 PF06886 392 - 466 9.1e-25 Targeting protein for Xklp2 (TPX2) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 390
AccB7I CCANNNNNTGG 1 cut(s) 587
AccBSI CCGCTC 1 cut(s) 29
AccII CGCG 1 cut(s) 248
AciI CCGC 7 cut(s) 29, 158, 683, 823, 958, 1078, 1192
AclWI GGATC 3 cut(s) 57, 217, 825
AcsI RAATTY 2 cut(s) 97, 307
AcuI CTGAAG 2 cut(s) 813, 1193
AcyI GRCGYC 3 cut(s) 179, 350, 387
AfeI AGCGCT 1 cut(s) 979
AfiI CCNNNNNNNGG 2 cut(s) 175, 587
AgsI TTSAA 5 cut(s) 370, 406, 482, 870, 991
AjnI CCWGG 2 cut(s) 118, 721
AloI GAACNNNNNNTCC 4 cut(s) 221, 253, 1016, 1048
AluBI AGCT 9 cut(s) 422, 625, 642, 912, 994, 1177, 1208, 1324, 1473
AluI AGCT 9 cut(s) 422, 625, 642, 912, 994, 1177, 1208, 1324, 1473
Alw21I GWGCWC 1 cut(s) 1475
Alw26I GTCTC 3 cut(s) 63, 838, 1267
AlwI GGATC 3 cut(s) 57, 217, 825
Ama87I CYCGRG 1 cut(s) 82
Aor51HI AGCGCT 1 cut(s) 979
AoxI GGCC 4 cut(s) 121, 824, 1096, 1361
ApeKI GCWGC 5 cut(s) 625, 680, 730, 1075, 1449
ApoI RAATTY 2 cut(s) 97, 307
Asp700I GAANNNNTTC 1 cut(s) 865
AspLEI GCGC 2 cut(s) 748, 980
AspS9I GGNCC 4 cut(s) 122, 469, 502, 1096
AsuC2I CCSGG 4 cut(s) 197, 214, 227, 506
AsuHPI GGTGA 3 cut(s) 43, 506, 607
AsuII TTCGAA 2 cut(s) 101, 311
AvaI CYCGRG 1 cut(s) 82
AvaII GGWCC 2 cut(s) 469, 502
BanII GRGCYC 1 cut(s) 1475
BbsI GAAGAC 1 cut(s) 1021
Bbv12I GWGCWC 1 cut(s) 1475
BbvI GCAGC 5 cut(s) 612, 692, 742, 1087, 1436
BccI CCATC 5 cut(s) 479, 842, 939, 1054, 1255
BciT130I CCWGG 2 cut(s) 120, 723
BciVI GTATCC 1 cut(s) 1074
BclI TGATCA 1 cut(s) 1216
BcnI CCSGG 4 cut(s) 197, 214, 227, 506
BcoDI GTCTC 3 cut(s) 63, 838, 1267
BfaI CTAG 2 cut(s) 924, 975
BfmI CTRYAG 1 cut(s) 1277
BfoI RGCGCY 1 cut(s) 981
BfuI GTATCC 1 cut(s) 1074
BisI GCNGC 8 cut(s) 159, 626, 681, 731, 824, 959, 1076, 1450
BlpI GCTNAGC 2 cut(s) 423, 981
BlsI GCNGC 8 cut(s) 160, 627, 682, 732, 825, 960, 1077, 1451
Bme1390I CCNGG 6 cut(s) 120, 197, 214, 227, 506, 723
Bme18I GGWCC 2 cut(s) 469, 502
BmeT110I CYCGRG 1 cut(s) 82
BmgT120I GGNCC 4 cut(s) 122, 469, 502, 1096
BmiI GGNNCC 2 cut(s) 124, 504
BmrFI CCNGG 6 cut(s) 120, 197, 214, 227, 506, 723
BmsI GCATC 3 cut(s) 588, 1335, 1399
BpiI GAAGAC 1 cut(s) 1021
BpmI CTGGAG 1 cut(s) 455
Bpu10I CCTNAGC 1 cut(s) 1092
Bpu1102I GCTNAGC 2 cut(s) 423, 981
Bpu14I TTCGAA 2 cut(s) 101, 311
BpuMI CCSGG 4 cut(s) 197, 214, 227, 506
BsaHI GRCGYC 3 cut(s) 179, 350, 387
BsaI GGTCTC 1 cut(s) 63
BsaJI CCNNGG 3 cut(s) 118, 212, 1103
BsaWI WCCGGW 1 cut(s) 1141
BsaXI ACNNNNNCTCC 4 cut(s) 221, 251, 1016, 1046
Bsc4I CCNNNNNNNGG 2 cut(s) 175, 587
Bse1I ACTGG 1 cut(s) 438
Bse3DI GCAATG 1 cut(s) 716
BseBI CCWGG 2 cut(s) 120, 723
BseDI CCNNGG 3 cut(s) 118, 212, 1103
BseGI GGATG 3 cut(s) 661, 853, 1390
BseLI CCNNNNNNNGG 2 cut(s) 175, 587
BseMI GCAATG 1 cut(s) 716
BseMII CTCAG 4 cut(s) 84, 414, 1106, 1200
BseNI ACTGG 1 cut(s) 438
BseRI GAGGAG 7 cut(s) 464, 686, 689, 726, 1079, 1082, 1310
BseXI GCAGC 5 cut(s) 612, 692, 742, 1087, 1436
Bsh1236I CGCG 1 cut(s) 248
BshFI GGCC 4 cut(s) 123, 826, 1098, 1363
BsiHKAI GWGCWC 1 cut(s) 1475
BsiHKCI CYCGRG 1 cut(s) 82
BsiSI CCGG 7 cut(s) 24, 197, 214, 226, 506, 749, 1142
BslFI GGGAC 3 cut(s) 151, 560, 1313
BslI CCNNNNNNNGG 2 cut(s) 175, 587
BsmAI GTCTC 3 cut(s) 63, 838, 1267
BsmFI GGGAC 3 cut(s) 151, 560, 1313
BsmI GAATGC 1 cut(s) 748
BsnI GGCC 4 cut(s) 123, 826, 1098, 1363
Bso31I GGTCTC 1 cut(s) 63
BsoBI CYCGRG 1 cut(s) 82
Bsp119I TTCGAA 2 cut(s) 101, 311
Bsp1286I GDGCHC 1 cut(s) 1475
Bsp143I GATC 6 cut(s) 9, 62, 222, 817, 1216, 1440
Bsp1720I GCTNAGC 2 cut(s) 423, 981
Bsp19I CCATGG 1 cut(s) 1103
Bsp68I TCGCGA 1 cut(s) 248
BspACI CCGC 7 cut(s) 29, 158, 683, 823, 958, 1078, 1192
BspANI GGCC 4 cut(s) 123, 826, 1098, 1363
BspCNI CTCAG 4 cut(s) 85, 415, 1105, 1201
BspFNI CGCG 1 cut(s) 248
BspLI GGNNCC 2 cut(s) 124, 504
BspMAI CTGCAG 1 cut(s) 1281
BspPI GGATC 3 cut(s) 57, 217, 825
BspT104I TTCGAA 2 cut(s) 101, 311
BspTNI GGTCTC 1 cut(s) 63
BsrBI CCGCTC 1 cut(s) 29
BsrDI GCAATG 1 cut(s) 716
BsrI ACTGG 1 cut(s) 438
BssECI CCNNGG 3 cut(s) 118, 212, 1103
BssMI GATC 6 cut(s) 9, 62, 222, 817, 1216, 1440
BssNI GRCGYC 3 cut(s) 179, 350, 387
BssT1I CCWWGG 1 cut(s) 1103
Bst2UI CCWGG 2 cut(s) 120, 723
Bst4CI ACNGT 1 cut(s) 1184
Bst6I CTCTTC 7 cut(s) 354, 555, 566, 681, 841, 1023, 1287
BstACI GRCGYC 3 cut(s) 179, 350, 387
BstBI TTCGAA 2 cut(s) 101, 311
BstC8I GCNNGC 4 cut(s) 286, 1130, 1194, 1206
BstDEI CTNAG 6 cut(s) 93, 423, 981, 995, 1092, 1209
BstDSI CCRYGG 1 cut(s) 1103
BstF5I GGATG 3 cut(s) 661, 853, 1390
BstFNI CGCG 1 cut(s) 248
BstH2I RGCGCY 1 cut(s) 981
BstHHI GCGC 2 cut(s) 748, 980
BstKTI GATC 6 cut(s) 12, 65, 225, 820, 1219, 1443
BstMAI GTCTC 3 cut(s) 63, 838, 1267
BstMBI GATC 6 cut(s) 9, 62, 222, 817, 1216, 1440
BstMWI GCNNNNNNNGC 4 cut(s) 143, 727, 977, 991
BstNI CCWGG 2 cut(s) 120, 723
BstNSI RCATGY 1 cut(s) 1192
BstSCI CCNGG 6 cut(s) 118, 195, 212, 225, 504, 721
BstSFI CTRYAG 1 cut(s) 1277
BstUI CGCG 1 cut(s) 248
BstV1I GCAGC 5 cut(s) 612, 692, 742, 1087, 1436
BstV2I GAAGAC 1 cut(s) 1021
BstX2I RGATCY 1 cut(s) 222
BstYI RGATCY 1 cut(s) 222
BsuI GTATCC 1 cut(s) 1074
BsuRI GGCC 4 cut(s) 123, 826, 1098, 1363
BtgI CCRYGG 1 cut(s) 1103
BtsCI GGATG 3 cut(s) 661, 853, 1390
BtsI GCAGTG 1 cut(s) 132
BtsIMutI CAGTG 3 cut(s) 90, 132, 1189
BtuMI TCGCGA 1 cut(s) 248
Cac8I GCNNGC 4 cut(s) 286, 1130, 1194, 1206
CfoI GCGC 2 cut(s) 748, 980
Cfr13I GGNCC 4 cut(s) 122, 469, 502, 1096
CseI GACGC 3 cut(s) 135, 168, 339
CviAII CATG 5 cut(s) 53, 1104, 1189, 1421, 1458
DdeI CTNAG 6 cut(s) 93, 423, 981, 995, 1092, 1209
DpnI GATC 6 cut(s) 11, 64, 224, 819, 1218, 1442
DpnII GATC 6 cut(s) 9, 62, 222, 817, 1216, 1440
Eam1104I CTCTTC 7 cut(s) 354, 555, 566, 681, 841, 1023, 1287
EarI CTCTTC 7 cut(s) 354, 555, 566, 681, 841, 1023, 1287
Ecl136II GAGCTC 1 cut(s) 1473
Eco130I CCWWGG 1 cut(s) 1103
Eco147I AGGCCT 1 cut(s) 1363
Eco24I GRGCYC 1 cut(s) 1475
Eco31I GGTCTC 1 cut(s) 63
Eco47I GGWCC 2 cut(s) 469, 502
Eco47III AGCGCT 1 cut(s) 979
Eco53kI GAGCTC 1 cut(s) 1473
Eco57I CTGAAG 2 cut(s) 813, 1193
Eco88I CYCGRG 1 cut(s) 82
EcoICRI GAGCTC 1 cut(s) 1473
EcoO109I RGGNCCY 2 cut(s) 469, 1096
EcoRI GAATTC 1 cut(s) 97
EcoRII CCWGG 2 cut(s) 118, 721
EcoT14I CCWWGG 1 cut(s) 1103
EcoT22I ATGCAT 1 cut(s) 603
EcoT38I GRGCYC 1 cut(s) 1475
ErhI CCWWGG 1 cut(s) 1103
FaeI CATG 5 cut(s) 56, 1107, 1192, 1424, 1461
FaiI YATR 9 cut(s) 54, 336, 477, 1105, 1190, 1400, 1422, 1459, 1469
FalI AAGNNNNNCTT 4 cut(s) 862, 894, 1436, 1468
FaqI GGGAC 3 cut(s) 151, 560, 1313
FatI CATG 5 cut(s) 52, 1103, 1188, 1420, 1457
FauI CCCGC 1 cut(s) 1185
FbaI TGATCA 1 cut(s) 1216
Fnu4HI GCNGC 8 cut(s) 159, 626, 681, 731, 824, 959, 1076, 1450
FokI GGATG 3 cut(s) 668, 860, 1377
FriOI GRGCYC 1 cut(s) 1475
Fsp4HI GCNGC 8 cut(s) 159, 626, 681, 731, 824, 959, 1076, 1450
FspBI CTAG 2 cut(s) 924, 975
GlaI GCGC 2 cut(s) 747, 979
GluI GCNGC 8 cut(s) 159, 626, 681, 731, 824, 959, 1076, 1450
GsuI CTGGAG 1 cut(s) 455
HaeII RGCGCY 1 cut(s) 981
HaeIII GGCC 4 cut(s) 123, 826, 1098, 1363
HapII CCGG 7 cut(s) 24, 197, 214, 226, 506, 749, 1142
HgaI GACGC 3 cut(s) 135, 168, 339
HhaI GCGC 2 cut(s) 748, 980
Hin1I GRCGYC 3 cut(s) 179, 350, 387
Hin1II CATG 5 cut(s) 56, 1107, 1192, 1424, 1461
Hin6I GCGC 2 cut(s) 746, 978
HinP1I GCGC 2 cut(s) 746, 978
HinfI GANTC 9 cut(s) 34, 271, 392, 463, 607, 634, 650, 805, 917
HpaII CCGG 7 cut(s) 24, 197, 214, 226, 506, 749, 1142
HphI GGTGA 3 cut(s) 43, 506, 607
Hpy166II GTNNAC 2 cut(s) 431, 714
Hpy188I TCNGA 7 cut(s) 276, 397, 655, 741, 784, 802, 916
Hpy188III TCNNGA 8 cut(s) 60, 84, 247, 370, 467, 535, 815, 1036
Hpy8I GTNNAC 2 cut(s) 431, 714
Hpy99I CGWCG 9 cut(s) 151, 245, 248, 355, 386, 389, 392, 769, 772
HpyAV CCTTC 9 cut(s) 259, 295, 400, 482, 537, 586, 694, 837, 1371
HpyCH4III ACNGT 1 cut(s) 1184
HpyCH4IV ACGT 1 cut(s) 387
HpyCH4V TGCA 5 cut(s) 288, 294, 601, 730, 1279
HpyF10VI GCNNNNNNNGC 4 cut(s) 143, 727, 977, 991
HpyF3I CTNAG 6 cut(s) 93, 423, 981, 995, 1092, 1209
HpySE526I ACGT 1 cut(s) 387
Hsp92I GRCGYC 3 cut(s) 179, 350, 387
Hsp92II CATG 5 cut(s) 56, 1107, 1192, 1424, 1461
HspAI GCGC 2 cut(s) 746, 978
Ksp22I TGATCA 1 cut(s) 1216
Kzo9I GATC 6 cut(s) 9, 62, 222, 817, 1216, 1440
LmnI GCTCC 4 cut(s) 26, 1182, 1321, 1478
Lsp1109I GCAGC 5 cut(s) 612, 692, 742, 1087, 1436
LweI GCATC 3 cut(s) 588, 1335, 1399
MaeI CTAG 2 cut(s) 924, 975
MaeII ACGT 1 cut(s) 387
MaeIII GTNAC 3 cut(s) 31, 1044, 1184
MalI GATC 6 cut(s) 11, 64, 224, 819, 1218, 1442
MbiI CCGCTC 1 cut(s) 29
MboI GATC 6 cut(s) 9, 62, 222, 817, 1216, 1440
MfeI CAATTG 1 cut(s) 1086
MflI RGATCY 1 cut(s) 222
MhlI GDGCHC 1 cut(s) 1475
MluCI AATT 5 cut(s) 97, 307, 515, 1086, 1152
MlyI GAGTC 1 cut(s) 28
MmeI TCCRAC 2 cut(s) 537, 1418
Mph1103I ATGCAT 1 cut(s) 603
MroXI GAANNNNTTC 1 cut(s) 865
MspA1I CMGCKG 2 cut(s) 683, 1078
MspI CCGG 7 cut(s) 24, 197, 214, 226, 506, 749, 1142
MspR9I CCNGG 6 cut(s) 120, 197, 214, 227, 506, 723
MunI CAATTG 1 cut(s) 1086
Mva1269I GAATGC 1 cut(s) 748
MvaI CCWGG 2 cut(s) 120, 723
MvnI CGCG 1 cut(s) 248
MwoI GCNNNNNNNGC 4 cut(s) 143, 727, 977, 991
NciI CCSGG 4 cut(s) 197, 214, 227, 506
NcoI CCATGG 1 cut(s) 1103
NdeII GATC 6 cut(s) 9, 62, 222, 817, 1216, 1440
NlaIII CATG 5 cut(s) 56, 1107, 1192, 1424, 1461
NlaIV GGNNCC 2 cut(s) 124, 504
NmuCI GTSAC 2 cut(s) 31, 1184
NruI TCGCGA 1 cut(s) 248
NsiI ATGCAT 1 cut(s) 603
NspI RCATGY 1 cut(s) 1192
NspV TTCGAA 2 cut(s) 101, 311
PaeR7I CTCGAG 1 cut(s) 82
PceI AGGCCT 1 cut(s) 1363
PcsI WCGNNNNNNNCGW 3 cut(s) 387, 393, 776
PctI GAATGC 1 cut(s) 748
PdmI GAANNNNTTC 1 cut(s) 865
PfeI GAWTC 8 cut(s) 271, 392, 463, 607, 634, 650, 805, 917
PflFI GACNNNGTC 1 cut(s) 386
PflMI CCANNNNNTGG 1 cut(s) 587
PfoI TCCNGGA 1 cut(s) 225
PkrI GCNGC 8 cut(s) 160, 627, 682, 732, 825, 960, 1077, 1451
PleI GAGTC 1 cut(s) 28
PpsI GAGTC 1 cut(s) 28
PpuMI RGGWCCY 1 cut(s) 469
Psp124BI GAGCTC 1 cut(s) 1475
Psp5II RGGWCCY 1 cut(s) 469
Psp6I CCWGG 2 cut(s) 118, 721
PspGI CCWGG 2 cut(s) 118, 721
PspN4I GGNNCC 2 cut(s) 124, 504
PspPI GGNCC 4 cut(s) 122, 469, 502, 1096
PspPPI RGGWCCY 1 cut(s) 469
PstI CTGCAG 1 cut(s) 1281
PsuI RGATCY 1 cut(s) 222
PsyI GACNNNGTC 1 cut(s) 386
RruI TCGCGA 1 cut(s) 248
SacI GAGCTC 1 cut(s) 1475
SatI GCNGC 8 cut(s) 159, 626, 681, 731, 824, 959, 1076, 1450
Sau3AI GATC 6 cut(s) 9, 62, 222, 817, 1216, 1440
Sau96I GGNCC 4 cut(s) 122, 469, 502, 1096
SchI GAGTC 1 cut(s) 28
ScrFI CCNGG 6 cut(s) 120, 197, 214, 227, 506, 723
SduI GDGCHC 1 cut(s) 1475
SfaNI GCATC 3 cut(s) 588, 1335, 1399
SfcI CTRYAG 1 cut(s) 1277
Sfr274I CTCGAG 1 cut(s) 82
SfuI TTCGAA 2 cut(s) 101, 311
SinI GGWCC 2 cut(s) 469, 502
SlaI CTCGAG 1 cut(s) 82
SmlI CTYRAG 1 cut(s) 82
SmoI CTYRAG 1 cut(s) 82
Sse9I AATT 5 cut(s) 97, 307, 515, 1086, 1152
SseBI AGGCCT 1 cut(s) 1363
SsiI CCGC 7 cut(s) 29, 158, 683, 823, 958, 1078, 1192
SspMI CTAG 2 cut(s) 924, 975
SstI GAGCTC 1 cut(s) 1475
StuI AGGCCT 1 cut(s) 1363
StyD4I CCNGG 6 cut(s) 118, 195, 212, 225, 504, 721
StyI CCWWGG 1 cut(s) 1103
TaaI ACNGT 1 cut(s) 1184
TaiI ACGT 1 cut(s) 390
TaqII GACCGA 1 cut(s) 800
TasI AATT 5 cut(s) 97, 307, 515, 1086, 1152
TauI GCSGC 3 cut(s) 161, 826, 961
TfiI GAWTC 8 cut(s) 271, 392, 463, 607, 634, 650, 805, 917
TscAI CASTG 3 cut(s) 97, 139, 1189
TseFI GTSAC 2 cut(s) 31, 1184
TseI GCWGC 5 cut(s) 625, 680, 730, 1075, 1449
Tsp45I GTSAC 2 cut(s) 31, 1184
TspDTI ATGAA 5 cut(s) 351, 464, 1357, 1400, 1409
TspRI CASTG 3 cut(s) 97, 139, 1189
Tth111I GACNNNGTC 1 cut(s) 386
Van91I CCANNNNNTGG 1 cut(s) 587
VpaK11BI GGWCC 2 cut(s) 469, 502
XapI RAATTY 2 cut(s) 97, 307
XceI RCATGY 1 cut(s) 1192
XhoI CTCGAG 1 cut(s) 82
XmnI GAANNNNTTC 1 cut(s) 865
XspI CTAG 2 cut(s) 924, 975
ZraI GACGTC 1 cut(s) 388
Zsp2I ATGCAT 1 cut(s) 603
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.