MD00G1006000.v1.1

Targeting protein for Xklp2 (TPX2)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0004726
Physical Location & Seq
Reverse (-)
820054 .. 821298
1245 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1006000.v1.1.491

Sequence Viewer

Length: 408 bp
ATGCATTTTGTTCAGGCGTTTGAGAAGTTGCTTTCGATTCCGAGCTCGAAGGATTCGGATGAGCAGAATGGGGAGGAGGAAGCGGCGGAAGAGATTGGGAAGAAGGTGGTGAAGTGGGCATTACCCGGATTGCAACCTCAGCCTAAGGCGTCGGAAACACAGGTCTCATCTTATTCGTTTTTCTCGTCGGACTTGTTCTTGACGTCAGATAATCTTGGTCTGGATTGGCGAACTTTAGTCTCTTCTTCTTGGGATGGAAGTCTGGGAAGTGTCTCAAACAGGACTTTGAATTTTGAGGGGGGTGCAAAGGAGCCGAAGAAATCTCTGAATCTTCTAGCACAATCGGATAAGGGAGATGGAAGAAGAAGAAGCAACAGAGAGACACTAGCGCTAAGCCATTCAAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPX2

Protein Analysis

136

Amino Acids

14.81

Weight (kDa)

5.41

Isoelectric Point (pI)

52.58

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 206
AciI CCGC 2 cut(s) 83, 86
AcsI RAATTY 1 cut(s) 289
AcyI GRCGYC 2 cut(s) 149, 203
AfeI AGCGCT 1 cut(s) 390
AgsI TTSAA 2 cut(s) 289, 402
AluBI AGCT 2 cut(s) 45, 405
AluI AGCT 2 cut(s) 45, 405
Alw21I GWGCWC 1 cut(s) 47
Alw26I GTCTC 4 cut(s) 169, 244, 277, 374
Aor51HI AGCGCT 1 cut(s) 390
ApoI RAATTY 1 cut(s) 289
AspLEI GCGC 1 cut(s) 391
AsuC2I CCSGG 1 cut(s) 126
AsuHPI GGTGA 1 cut(s) 121
AxyI CCTNAGG 1 cut(s) 144
BanII GRGCYC 1 cut(s) 47
Bbv12I GWGCWC 1 cut(s) 47
BbvCI CCTCAGC 1 cut(s) 138
BccI CCATC 2 cut(s) 248, 350
BcnI CCSGG 1 cut(s) 126
BcoDI GTCTC 4 cut(s) 169, 244, 277, 374
BfaI CTAG 2 cut(s) 335, 386
BfoI RGCGCY 1 cut(s) 392
BisI GCNGC 1 cut(s) 84
BlpI GCTNAGC 1 cut(s) 392
BlsI GCNGC 1 cut(s) 85
Bme1390I CCNGG 1 cut(s) 126
BmiI GGNNCC 1 cut(s) 312
BmrFI CCNGG 1 cut(s) 126
Bpu10I CCTNAGC 1 cut(s) 138
Bpu1102I GCTNAGC 1 cut(s) 392
BpuMI CCSGG 1 cut(s) 126
BsaHI GRCGYC 2 cut(s) 149, 203
BsaI GGTCTC 1 cut(s) 169
Bse21I CCTNAGG 1 cut(s) 144
BseGI GGATG 2 cut(s) 64, 259
BseMII CTCAG 1 cut(s) 152
BseRI GAGGAG 1 cut(s) 89
BsiHKAI GWGCWC 1 cut(s) 47
BsiSI CCGG 1 cut(s) 126
BsmAI GTCTC 4 cut(s) 169, 244, 277, 374
Bso31I GGTCTC 1 cut(s) 169
Bsp1286I GDGCHC 1 cut(s) 47
Bsp1720I GCTNAGC 1 cut(s) 392
BspACI CCGC 2 cut(s) 83, 86
BspCNI CTCAG 1 cut(s) 151
BspLI GGNNCC 1 cut(s) 312
BspTNI GGTCTC 1 cut(s) 169
BssNI GRCGYC 2 cut(s) 149, 203
Bst6I CTCTTC 2 cut(s) 84, 247
BstACI GRCGYC 2 cut(s) 149, 203
BstDEI CTNAG 3 cut(s) 138, 144, 392
BstF5I GGATG 2 cut(s) 64, 259
BstH2I RGCGCY 1 cut(s) 392
BstHHI GCGC 1 cut(s) 391
BstMAI GTCTC 4 cut(s) 169, 244, 277, 374
BstMWI GCNNNNNNNGC 2 cut(s) 139, 402
BstSCI CCNGG 1 cut(s) 124
Bsu36I CCTNAGG 1 cut(s) 144
BtsCI GGATG 2 cut(s) 64, 259
CfoI GCGC 1 cut(s) 391
CseI GACGC 1 cut(s) 138
CviJI RGCY 5 cut(s) 45, 142, 313, 396, 405
CviKI_1 RGCY 5 cut(s) 45, 142, 313, 396, 405
DdeI CTNAG 3 cut(s) 138, 144, 392
Eam1104I CTCTTC 2 cut(s) 84, 247
EarI CTCTTC 2 cut(s) 84, 247
EciI GGCGGA 1 cut(s) 101
Ecl136II GAGCTC 1 cut(s) 45
Eco24I GRGCYC 1 cut(s) 47
Eco31I GGTCTC 1 cut(s) 169
Eco47III AGCGCT 1 cut(s) 390
Eco53kI GAGCTC 1 cut(s) 45
Eco81I CCTNAGG 1 cut(s) 144
EcoICRI GAGCTC 1 cut(s) 45
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 1 cut(s) 47
Fnu4HI GCNGC 1 cut(s) 84
FokI GGATG 2 cut(s) 71, 266
FriOI GRGCYC 1 cut(s) 47
Fsp4HI GCNGC 1 cut(s) 84
FspBI CTAG 2 cut(s) 335, 386
GlaI GCGC 1 cut(s) 390
GluI GCNGC 1 cut(s) 84
HaeII RGCGCY 1 cut(s) 392
HapII CCGG 1 cut(s) 126
HgaI GACGC 1 cut(s) 138
HhaI GCGC 1 cut(s) 391
Hin1I GRCGYC 2 cut(s) 149, 203
Hin6I GCGC 1 cut(s) 389
HinP1I GCGC 1 cut(s) 389
HinfI GANTC 3 cut(s) 37, 53, 328
HpaII CCGG 1 cut(s) 126
HphI GGTGA 1 cut(s) 121
Hpy188I TCNGA 7 cut(s) 42, 58, 154, 190, 208, 327, 346
Hpy188III TCNNGA 2 cut(s) 199, 221
Hpy99I CGWCG 2 cut(s) 154, 190
HpyAV CCTTC 2 cut(s) 43, 97
HpyCH4IV ACGT 1 cut(s) 203
HpyCH4V TGCA 3 cut(s) 4, 133, 305
HpyF10VI GCNNNNNNNGC 2 cut(s) 139, 402
HpyF3I CTNAG 3 cut(s) 138, 144, 392
HpySE526I ACGT 1 cut(s) 203
Hsp92I GRCGYC 2 cut(s) 149, 203
HspAI GCGC 1 cut(s) 389
LmnI GCTCC 1 cut(s) 310
LpnPI CCDG 5 cut(s) 139, 146, 206, 248, 265
MaeI CTAG 2 cut(s) 335, 386
MaeII ACGT 1 cut(s) 203
MboII GAAGA 9 cut(s) 101, 112, 234, 237, 323, 328, 372, 375, 378
MhlI GDGCHC 1 cut(s) 47
MluCI AATT 1 cut(s) 289
MmeI TCCRAC 2 cut(s) 132, 168
MnlI CCTC 4 cut(s) 67, 70, 147, 289
Mph1103I ATGCAT 1 cut(s) 6
MspI CCGG 1 cut(s) 126
MspR9I CCNGG 1 cut(s) 126
MwoI GCNNNNNNNGC 2 cut(s) 139, 402
NciI CCSGG 1 cut(s) 126
NlaIV GGNNCC 1 cut(s) 312
NsiI ATGCAT 1 cut(s) 6
PcsI WCGNNNNNNNCGW 1 cut(s) 182
PfeI GAWTC 3 cut(s) 37, 53, 328
PkrI GCNGC 1 cut(s) 85
Psp124BI GAGCTC 1 cut(s) 47
PspN4I GGNNCC 1 cut(s) 312
SacI GAGCTC 1 cut(s) 47
SatI GCNGC 1 cut(s) 84
ScrFI CCNGG 1 cut(s) 126
SduI GDGCHC 1 cut(s) 47
SetI ASST 6 cut(s) 47, 108, 139, 165, 206, 407
Sse9I AATT 1 cut(s) 289
SsiI CCGC 2 cut(s) 83, 86
SspMI CTAG 2 cut(s) 335, 386
SstI GAGCTC 1 cut(s) 47
StyD4I CCNGG 1 cut(s) 124
TaiI ACGT 1 cut(s) 206
TaqI TCGA 2 cut(s) 35, 47
TasI AATT 1 cut(s) 289
TauI GCSGC 1 cut(s) 86
TfiI GAWTC 3 cut(s) 37, 53, 328
XapI RAATTY 1 cut(s) 289
XspI CTAG 2 cut(s) 335, 386
ZraI GACGTC 1 cut(s) 204
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.