Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0008446

Physical Location & Seq

Genomic Coordinates Reverse (-)

1985338 .. 1990245

Gene Structure 4908 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT3G06480.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3267 bp

ATGGCCACAACAGAAGATACTCCAGCTTCTGCTGGTCCAAGATATGCTCCAGAGGACCCAACTCTTCCCCAACCATGGAAGGGACTTATTGATGGAAGCACAGGAATTCTCTATTACTGGAATCCTGAAACCAATGTCACTCAGTATGAAAGACCTTCTGCGCCTCCTCCCCACTCTGCCACAACACCGAAGCTAGCTCAGATTCCTGTACCCTCTTCAGGACAAGGTCACCAAGCTCAGCATGAACAAGCAAAACCAGTTGGTCATGTGTCTCAGCAGCATGGTTTCCAACAACAGCCACAGCAATTTCCATCCCAACATGTAAGACCCCAAATGATGCAGCAGCATCCGGCCCAGCAAATGCCTCAACAATCAGGTCAACAGTTTCCCCAACAACAGAGCCAATCGATGGTGCCGCACCCACATGGGCATCCTTCTGTACAGACTTATCAGCCAACGACGCAGCAGCAGCAGCAAGGGATGCAGAATCAGCATTCACAAATGCCTCAGCAGCTGAGCCATCAGTATGCTCATTCACAACAACATTACATGGGTTTTCGGCCTCACATGCAAACGCAAGGACTTCAAAATTCACATCAGACACCTCAGGGTGGTCCCCATGGTCAGCAGTTTCCGAGCCAGCAAGAGTACAACTCATTGGCTCCGAAGAGGGAGGGGGATGAGTTCCATGGGGGTAAGAAAACTGGGTTTTCTCAACCCCATTTACCAAATTCTGAACGTTCACCATCTCAAAATACCCACTTTGAAGCCAATGCAGCATCACAAAAGACGAATGCAAACTTGGCTATGGCTCAGAAATGCAATGGACCTCAAGCAAATGCTGCAGTCACACAGTTTCAACAGCCAGGGGCGAACCTAATTCACCAGCAACTTGGACCTAGGGCGCCAAATCAAATGGACCAGACAATGTTGCACCAGAAGTCTCACGTTTCCCCTTTCCAATCAAATAATACTTATGAGAATAATCTACAATCTAGGCCTGGTAATGACTCGTACGTTAACATGAGAATGGAAGTGCCGGTTAGGGGTGCCCAACCACTTCATCCTGCAGCAATGCCTAAGGATATAAGGATAAGTGGTGGTCCACCAACAAATGCTGATCCCGCCATGGGGCAAACAGGGCATGGAACATATGGACACGCTGGTCCAGCTTTTCCAAATAAATCCTTGGTGCGGCCTCATTTTGTCACATCTCCTGATGTTCCCCATCTCTCTCCCGTTGAAATATACCGAAAACAACATGAAGTCACAACAACTGGCGAGAACATTCCAGCACCATACATCACATTTGAATCTAGTGGTCTCCCACCTGAAATCCTTAGAGAGTTGCTTTCTGCTGGATTTCCATCGCCAACACCGATCCAGGCCCAAACATGGCCAATTGCTCTGCAAAGCAGGGATATAGTTGCAATTGCAAAAACTGGTTCTGGTAAAACATTGGGATACTTGATCCCTGCCTTCATTCTTCTAAGGCACTGTCGTAACGACTCCCGCAATGGCCCCACAGTTTTGATCCTAGCTCCTACTCGGGAGCTAGCCACACAAATACAAGACGAAGCACTAAGGTTTGGACGGTCTTCCAGGATATCATGCACTTGTTTGTATGGTGGAGCTCCAAAGGGTCCTCAATTGAAAGAGTTGGAACGAGGAGCTGATATTGTGGTGGCAACTCCTGGGCGTCTAAATGATATACTGGAGATGAAGATGATTGATTTCCAGCAGGTTTCCCTTCTTGTGCTTGACGAGGCAGACCGAATGCTTGACATGGGTTTTGAACCCCAAATCCGTAAGATTGTGAATGAAATACCTCCTCGGAGACAGACTCTTATGTACACAGCCACTTGGCCAAAAGAAGTAAGAAAAATTGCAAGCGATCTTCTTGTGAACCCTGTCCAAGTTAACATAGGCAGGGTAGATGAGTTGGCTGCTAACAAAGCAATAACGCAGTATGTTGAAGTAGTCCCACAAATGGAGAAAGAGAGACGTTTGGAGCAAATCCTTAGGTCACAAGAACGAGGTTCAAAAGTTATAATATTTTGCTCCACGAAGAGGCTTTGTGACCATCTTGCACGTAGTGTCGGACGTCATTTTGGCGCTGTTGTGATCCATGGAGACAAGACTCAAGGTGAGAGAGATTGGGTGCTTAACCAGTTCCGAAGCGGGAAGTCTTGTGTACTGATAGCTACTGACGTTGCTGCCCGGGGACTCGACATAAAAGACATACGAGTTGTGATCAACTACGATTTTCCAACTGGAGTTGAAGACTATGTCCACCGTATTGGGAGAACCGGTCGAGCTGGTGCAACTGGAGTTGCATTCACTTTCTTTACAGAGCAAGATTGGAAATACGCACCTGATTTGATCAAAGTCCTGGAAGGAGCGAACCAGCAAGTACCGCCTCAAGTAAGAGATATTGCAATGCGTGGTGGTGGTGGTGGTGGTCCCGGTTATAGCCAGGATCGAAGAGGTATGGTTAACCGGTTTGACTCTGGCGGTGGTGGTACCCGTTGGGATTCTGGTGGTGGGTTTGGTGGGCGTGGTGGTGGGTTTAGTGGCCGTGAGGGTGGGTTTGGAGGCCGTGAGGGTGGATTTGGGGGCCGTGAGGGTGGATTTGGAGGCCGTGGGGGAAGATTTGGAATGAGAGATGACTCATTTGGACGTGGCGGGAATCGTGGTCGGGGTTTTACTGGTCCTGATGCTGGTCATATGAATGTGGGTGGTAGAGGCGGATTTGGTCGGTTTGGCAATAATAATAATATGGAGAGCCGAGGCTTTGGTCGTGGTAGTGGTAGAGGCTTTGGTCGTGGTGTTGGACGGTTTGACAACAGAAGAGGTAGAAGTCGCAGCAGAAGTCCTGATTTGGTTCGGCCACGGCGCCGTAGCTCGAGCTATAGCCGGAGTCGAAGCCGGAGCGGGAGCTATAGTCGTAGCCGTAGCCGTAGCAGGAGCTGGTCACGTTCAAGGAGTCGCAGCCCTAGACATAGCCGTGACCGAGGTGGTCATAACAGGAGCAGAAGCTATAGCCGTAGTCCTAGTCCGGTTTATGAAAGACGAGATAGAGCGCCACGTGTTTCTGGGTTTGATATTAAACCACCAGTTGAATCTGTAGTGAACCTGGACATGAATGCCGCGGCAGCGATTGAAAACGTGGTTCCTACATCCTTGTCTGAGAGACAAGGGAATGGGGTTGTTGAATCGGAGGTAGAAGCGGCTTTAGTACGACCAGTGGTTGATGAAGAACCCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005773 GO:0006139 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009987 GO:0010501 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031974 GO:0031981 GO:0034641 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0070013 GO:0070035 GO:0071704 GO:0090304 GO:0140098 GO:1901360

KEGG Pathways

Metabolic & Signaling

ko03040 ko05202 ko05205 map03040 map05202 map05205

Pfam Domains

Protein Families

DEAD Helicase_C WW

Protein Analysis

1088

Amino Acids

119.56

Weight (kDa)

9.95

Isoelectric Point (pI)

62.46

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WW	PF00397	22 - 52	1.1e-07	WW domain
DEAD	PF00270	459 - 628	4.2e-49	DEAD/DEAH box helicase
ResIII	PF04851	471 - 622	3.2e-08	Type III restriction enzyme, res subunit
Helicase_C	PF00271	666 - 753	3.9e-21	Helicase conserved C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0008446)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G06480 AT3G06480
fragaria_vesca	FvH4_4g21040
malus_domestica	MD13G1145900.v1.1 MD16G1139800.v1.1 MD16G1139900.v1.1
prunus_persica	Prupe.1G109600_v2.0.a1
pyrus_communis	pycom13g12540 pycom16g12400
rosa_chinensis	RchiOBHm_Chr2g0174651 RchiOBHm_Chr4g0426571
rosa_laevigata	RLG00000007289
rosa_multiflora	Rmu_co8268839.1_g000001 Rmu_sc0001821.1_g000009 Rmu_sc0007189.1_g000006
rosa_roxburghii	Rroxscaffold_5G00368580
rosa_rugosa	Rorug04G0213500
rosa_samantha	Rh4AG268700 Rh4BG275000 Rh4CG289300 Rh4DG271800
rosa_wichuraiana	Rw2G054030 Rw4G023310

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2051
AasI	GACNNNNNNGTC	1 cut(s)	1164
AatII	GACGTC	1 cut(s)	2107
Acc36I	ACCTGC	1 cut(s)	1732
Acc65I	GGTACC	1 cut(s)	2522
AccB1I	GGYRCC	5 cut(s)	412, 904, 1049, 2522, 2895
AccB7I	CCANNNNNTGG	1 cut(s)	409
AccBSI	CCGCTC	1 cut(s)	2934
AccII	CGCG	1 cut(s)	3152
AclI	AACGTT	1 cut(s)	739
AclWI	GGATC	6 cut(s)	1115, 1375, 1465, 1528, 2119, 2487
AcoI	YGGCCR	5 cut(s)	3, 1397, 1865, 2575, 2888
AcsI	RAATTY	3 cut(s)	105, 589, 730
AcuI	CTGAAG	1 cut(s)	201
AcvI	CACGTG	1 cut(s)	3089
AcyI	GRCGYC	4 cut(s)	905, 1699, 2104, 2896
AdeI	CACNNNGTG	1 cut(s)	2096
AfaI	GTAC	9 cut(s)	210, 441, 650, 1016, 1853, 2196, 2415, 2524, 3240
AflIII	ACRYGT	2 cut(s)	319, 3088
AgeI	ACCGGT	2 cut(s)	2309, 2499
AjiI	CACGTC	1 cut(s)	2681
AjnI	CCWGG	8 cut(s)	865, 1000, 1383, 1601, 1693, 2391, 2475, 3135
AjuI	GAANNNNNNNTTGG	2 cut(s)	785, 817
AloI	GAACNNNNNNTCC	2 cut(s)	1788, 1820
Alw21I	GWGCWC	1 cut(s)	1636
Alw26I	GTCTC	7 cut(s)	276, 948, 1328, 1831, 1996, 2127, 3187
AlwI	GGATC	6 cut(s)	1115, 1375, 1465, 1528, 2119, 2487
AlwNI	CAGNNNCTG	3 cut(s)	29, 514, 2970
Ama87I	CYCGRG	3 cut(s)	1548, 2220, 2905
ApoI	RAATTY	3 cut(s)	105, 589, 730
ArsI	GACNNNNNNTTYG	2 cut(s)	1775, 1807
AsiGI	ACCGGT	2 cut(s)	2309, 2499
Asp718I	GGTACC	1 cut(s)	2522
AspA2I	CCTAGG	1 cut(s)	899
AspLEI	GCGC	5 cut(s)	163, 907, 2117, 2898, 3085
AsuC2I	CCSGG	3 cut(s)	2221, 2222, 2466
AsuHPI	GGTGA	4 cut(s)	221, 735, 875, 2159
AsuNHI	GCTAGC	2 cut(s)	193, 1555
AvaI	CYCGRG	3 cut(s)	1548, 2220, 2905
AvrII	CCTAGG	1 cut(s)	899
AxyI	CCTNAGG	3 cut(s)	606, 1080, 2021
BaeGI	GKGCMC	1 cut(s)	1054
BalI	TGGCCA	3 cut(s)	5, 1399, 1867
BanI	GGYRCC	5 cut(s)	412, 904, 1049, 2522, 2895
BanII	GRGCYC	1 cut(s)	1636
BbrPI	CACGTG	1 cut(s)	3089
BbsI	GAAGAC	2 cut(s)	1590, 2289
Bbv12I	GWGCWC	1 cut(s)	1636
BbvCI	CCTCAGC	1 cut(s)	507
BccI	CCATC	8 cut(s)	86, 319, 403, 528, 754, 1236, 1375, 2091
BciT130I	CCWGG	8 cut(s)	867, 1002, 1385, 1603, 1695, 2393, 2477, 3137
BciVI	GTATCC	1 cut(s)	1457
BclI	TGATCA	2 cut(s)	2253, 2382
BcnI	CCSGG	3 cut(s)	2221, 2222, 2466
BcoDI	GTCTC	7 cut(s)	276, 948, 1328, 1831, 1996, 2127, 3187
BfaI	CTAG	8 cut(s)	194, 900, 996, 1317, 1538, 1556, 2997, 3054
BfmI	CTRYAG	6 cut(s)	843, 1068, 2911, 2941, 3040, 3126
BfoI	RGCGCY	4 cut(s)	908, 2118, 2899, 3086
BfuAI	ACCTGC	1 cut(s)	1732
BfuI	GTATCC	1 cut(s)	1457
BlnI	CCTAGG	1 cut(s)	899
BlpI	GCTNAGC	2 cut(s)	237, 515
BmeT110I	CYCGRG	3 cut(s)	1548, 2220, 2905
BmgBI	CACGTC	1 cut(s)	2681
BmrI	ACTGGG	1 cut(s)	714
BmsI	GCATC	6 cut(s)	327, 355, 439, 471, 788, 2707
BmtI	GCTAGC	2 cut(s)	197, 1559
BmuI	ACTGGG	1 cut(s)	714
BpiI	GAAGAC	2 cut(s)	1590, 2289
BplI	GAGNNNNNCTC	4 cut(s)	638, 670, 1828, 1860
BpmI	CTGGAG	5 cut(s)	6, 33, 1736, 2295, 2349
Bpu10I	CCTNAGC	1 cut(s)	507
Bpu1102I	GCTNAGC	2 cut(s)	237, 515
BpuEI	CTTGAG	3 cut(s)	816, 2127, 2406
BpuMI	CCSGG	3 cut(s)	2221, 2222, 2466
Bsa29I	ATCGAT	1 cut(s)	407
BsaAI	YACGTR	2 cut(s)	2093, 3089
BsaBI	GATNNNNATC	1 cut(s)	1366
BsaHI	GRCGYC	4 cut(s)	905, 1699, 2104, 2896
BsaI	GGTCTC	1 cut(s)	1328
BsaWI	WCCGGW	3 cut(s)	2309, 2499, 3058
BsaXI	ACNNNNNCTCC	4 cut(s)	2296, 2326, 2929, 2959
Bse118I	RCCGGY	3 cut(s)	1039, 2309, 2499
Bse21I	CCTNAGG	3 cut(s)	606, 1080, 2021
Bse3DI	GCAATG	4 cut(s)	829, 1080, 1522, 2445
Bse8I	GATNNNNATC	1 cut(s)	1366
BseBI	CCWGG	8 cut(s)	867, 1002, 1385, 1603, 1695, 2393, 2477, 3137
BseCI	ATCGAT	1 cut(s)	407
BseGI	GGATG	7 cut(s)	311, 346, 430, 486, 685, 1063, 3179
BseJI	GATNNNNATC	1 cut(s)	1366
BseMI	GCAATG	4 cut(s)	829, 1080, 1522, 2445
BseMII	CTCAG	9 cut(s)	155, 212, 251, 287, 506, 521, 620, 827, 3180
BseRI	GAGGAG	3 cut(s)	156, 1683, 1821
BseSI	GKGCMC	1 cut(s)	1054
BseYI	CCCAGC	1 cut(s)	354
Bsh1236I	CGCG	1 cut(s)	3152
Bsh1285I	CGRYCG	1 cut(s)	2314
BshNI	GGYRCC	5 cut(s)	412, 904, 1049, 2522, 2895
BshTI	ACCGGT	2 cut(s)	2309, 2499
BshVI	ATCGAT	1 cut(s)	407
BsiEI	CGRYCG	1 cut(s)	2314
BsiHKAI	GWGCWC	1 cut(s)	1636
BsiHKCI	CYCGRG	3 cut(s)	1548, 2220, 2905
BsiSI	CCGG	9 cut(s)	350, 1040, 2221, 2310, 2466, 2500, 2917, 2929, 3059
BsiWI	CGTACG	1 cut(s)	1014
BslFI	GGGAC	5 cut(s)	96, 600, 1967, 2238, 2448
BsmAI	GTCTC	7 cut(s)	276, 948, 1328, 1831, 1996, 2127, 3187
BsmBI	CGTCTC	1 cut(s)	1996
BsmFI	GGGAC	5 cut(s)	96, 600, 1967, 2238, 2448
BsmI	GAATGC	5 cut(s)	493, 799, 1782, 2336, 3151
Bso31I	GGTCTC	1 cut(s)	1328
BsoBI	CYCGRG	3 cut(s)	1548, 2220, 2905
Bsp1286I	GDGCHC	2 cut(s)	1054, 1636
Bsp1407I	TGTACA	2 cut(s)	439, 1851
Bsp143I	GATC	9 cut(s)	1120, 1380, 1470, 1533, 1894, 2124, 2253, 2382, 2479
Bsp1720I	GCTNAGC	2 cut(s)	237, 515
Bsp19I	CCATGG	5 cut(s)	74, 619, 688, 1128, 2128
BspCNI	CTCAG	9 cut(s)	154, 211, 250, 286, 507, 520, 619, 826, 3181
BspDI	ATCGAT	1 cut(s)	407
BspFNI	CGCG	1 cut(s)	3152
BspMAI	CTGCAG	2 cut(s)	847, 1072
BspMI	ACCTGC	1 cut(s)	1732
BspOI	GCTAGC	2 cut(s)	197, 1559
BspPI	GGATC	6 cut(s)	1115, 1375, 1465, 1528, 2119, 2487
BspT107I	GGYRCC	5 cut(s)	412, 904, 1049, 2522, 2895
BspTNI	GGTCTC	1 cut(s)	1328
BsrBI	CCGCTC	1 cut(s)	2934
BsrDI	GCAATG	4 cut(s)	829, 1080, 1522, 2445
BsrFI	RCCGGY	3 cut(s)	1039, 2309, 2499
BsrGI	TGTACA	2 cut(s)	439, 1851
BssAI	RCCGGY	3 cut(s)	1039, 2309, 2499
BssMI	GATC	9 cut(s)	1120, 1380, 1470, 1533, 1894, 2124, 2253, 2382, 2479
BssNI	GRCGYC	4 cut(s)	905, 1699, 2104, 2896
BssT1I	CCWWGG	7 cut(s)	74, 619, 688, 899, 1128, 1188, 2128
Bst2UI	CCWGG	8 cut(s)	867, 1002, 1385, 1603, 1695, 2393, 2477, 3137
Bst4CI	ACNGT	7 cut(s)	384, 855, 1499, 1528, 1596, 2297, 2838
Bst6I	CTCTTC	6 cut(s)	69, 220, 662, 2063, 2479, 2845
BstACI	GRCGYC	4 cut(s)	905, 1699, 2104, 2896
BstAPI	GCANNNNNTGC	2 cut(s)	481, 842
BstAUI	TGTACA	2 cut(s)	439, 1851
BstBAI	YACGTR	2 cut(s)	2093, 3089
BstC8I	GCNNGC	4 cut(s)	195, 641, 1557, 1891
BstDSI	CCRYGG	8 cut(s)	74, 619, 688, 1128, 2128, 2641, 2891, 3150
BstEII	GGTNACC	1 cut(s)	227
BstF5I	GGATG	7 cut(s)	311, 346, 430, 486, 685, 1063, 3179
BstFNI	CGCG	1 cut(s)	3152
BstH2I	RGCGCY	4 cut(s)	908, 2118, 2899, 3086
BstHHI	GCGC	5 cut(s)	163, 907, 2117, 2898, 3085
BstKTI	GATC	9 cut(s)	1123, 1383, 1473, 1536, 1897, 2127, 2256, 2385, 2482
BstMAI	GTCTC	7 cut(s)	276, 948, 1328, 1831, 1996, 2127, 3187
BstMBI	GATC	9 cut(s)	1120, 1380, 1470, 1533, 1894, 2124, 2253, 2382, 2479
BstMCI	CGRYCG	1 cut(s)	2314
BstNI	CCWGG	8 cut(s)	867, 1002, 1385, 1603, 1695, 2393, 2477, 3137
BstNSI	RCATGY	2 cut(s)	323, 571
BstPI	GGTNACC	1 cut(s)	227
BstSFI	CTRYAG	6 cut(s)	843, 1068, 2911, 2941, 3040, 3126
BstSLI	GKGCMC	1 cut(s)	1054
BstUI	CGCG	1 cut(s)	3152
BstV2I	GAAGAC	2 cut(s)	1590, 2289
BstXI	CCANNNNNNTGG	2 cut(s)	893, 2300
Bsu15I	ATCGAT	1 cut(s)	407
Bsu36I	CCTNAGG	3 cut(s)	606, 1080, 2021
BsuI	GTATCC	1 cut(s)	1457
BsuTUI	ATCGAT	1 cut(s)	407
BtgI	CCRYGG	8 cut(s)	74, 619, 688, 1128, 2128, 2641, 2891, 3150
BtgZI	GCGATG	1 cut(s)	1353
BtrI	CACGTC	1 cut(s)	2681
BtsCI	GGATG	7 cut(s)	311, 346, 430, 486, 685, 1063, 3179
BtsIMutI	CAGTG	2 cut(s)	1495, 3252
BveI	ACCTGC	1 cut(s)	1732
Cac8I	GCNNGC	4 cut(s)	195, 641, 1557, 1891
CaiI	CAGNNNCTG	3 cut(s)	29, 514, 2970
CfoI	GCGC	5 cut(s)	163, 907, 2117, 2898, 3085
Cfr10I	RCCGGY	3 cut(s)	1039, 2309, 2499
Cfr42I	CCGCGG	1 cut(s)	3153
Cfr9I	CCCGGG	1 cut(s)	2220
ClaI	ATCGAT	1 cut(s)	407
CseI	GACGC	2 cut(s)	469, 1688
Csp6I	GTAC	9 cut(s)	209, 440, 649, 1015, 1852, 2195, 2414, 2523, 3239
CspAI	ACCGGT	2 cut(s)	2309, 2499
CviQI	GTAC	9 cut(s)	209, 440, 649, 1015, 1852, 2195, 2414, 2523, 3239
DinI	GGCGCC	2 cut(s)	906, 2897
DpnI	GATC	9 cut(s)	1122, 1382, 1472, 1535, 1896, 2126, 2255, 2384, 2481
DpnII	GATC	9 cut(s)	1120, 1380, 1470, 1533, 1894, 2124, 2253, 2382, 2479
DraIII	CACNNNGTG	1 cut(s)	2096
DrdI	GACNNNNNNGTC	1 cut(s)	1164
DseDI	GACNNNNNNGTC	1 cut(s)	1164
EaeI	YGGCCR	5 cut(s)	3, 1397, 1865, 2575, 2888
Eam1104I	CTCTTC	6 cut(s)	69, 220, 662, 2063, 2479, 2845
EarI	CTCTTC	6 cut(s)	69, 220, 662, 2063, 2479, 2845
EciI	GGCGGA	1 cut(s)	2763
Ecl136II	GAGCTC	1 cut(s)	1634
Eco130I	CCWWGG	7 cut(s)	74, 619, 688, 899, 1128, 1188, 2128
Eco147I	AGGCCT	1 cut(s)	1000
Eco24I	GRGCYC	1 cut(s)	1636
Eco31I	GGTCTC	1 cut(s)	1328
Eco32I	GATATC	1 cut(s)	1608
Eco53kI	GAGCTC	1 cut(s)	1634
Eco57I	CTGAAG	1 cut(s)	201
Eco72I	CACGTG	1 cut(s)	3089
Eco81I	CCTNAGG	3 cut(s)	606, 1080, 2021
Eco88I	CYCGRG	3 cut(s)	1548, 2220, 2905
Eco91I	GGTNACC	1 cut(s)	227
EcoICRI	GAGCTC	1 cut(s)	1634
EcoO109I	RGGNCCY	2 cut(s)	55, 1643
EcoO65I	GGTNACC	1 cut(s)	227
EcoRI	GAATTC	1 cut(s)	105
EcoRII	CCWGG	8 cut(s)	865, 1000, 1383, 1601, 1693, 2391, 2475, 3135
EcoRV	GATATC	1 cut(s)	1608
EcoT14I	CCWWGG	7 cut(s)	74, 619, 688, 899, 1128, 1188, 2128
EcoT38I	GRGCYC	1 cut(s)	1636
EgeI	GGCGCC	2 cut(s)	906, 2897
EheI	GGCGCC	2 cut(s)	906, 2897
ErhI	CCWWGG	7 cut(s)	74, 619, 688, 899, 1128, 1188, 2128
Esp3I	CGTCTC	1 cut(s)	1996
FaqI	GGGAC	5 cut(s)	96, 600, 1967, 2238, 2448
FauI	CCCGC	5 cut(s)	1132, 1520, 2174, 2678, 2927
FauNDI	CATATG	2 cut(s)	1153, 2727
FbaI	TGATCA	2 cut(s)	2253, 2382
FokI	GGATG	7 cut(s)	298, 333, 417, 493, 692, 1050, 3166
FriOI	GRGCYC	1 cut(s)	1636
FspBI	CTAG	8 cut(s)	194, 900, 996, 1317, 1538, 1556, 2997, 3054
GlaI	GCGC	5 cut(s)	162, 906, 2116, 2897, 3084
GsaI	CCCAGC	1 cut(s)	358
GsuI	CTGGAG	5 cut(s)	6, 33, 1736, 2295, 2349
HaeII	RGCGCY	4 cut(s)	908, 2118, 2899, 3086
HapII	CCGG	9 cut(s)	350, 1040, 2221, 2310, 2466, 2500, 2917, 2929, 3059
HgaI	GACGC	2 cut(s)	469, 1688
HhaI	GCGC	5 cut(s)	163, 907, 2117, 2898, 3085
Hin1I	GRCGYC	4 cut(s)	905, 1699, 2104, 2896
Hin6I	GCGC	5 cut(s)	161, 905, 2115, 2896, 3083
HinP1I	GCGC	5 cut(s)	161, 905, 2115, 2896, 3083
HincII	GTYRAC	4 cut(s)	380, 1021, 1921, 2497
HindII	GTYRAC	4 cut(s)	380, 1021, 1921, 2497
HpaI	GTTAAC	3 cut(s)	1021, 1921, 2497
HpaII	CCGG	9 cut(s)	350, 1040, 2221, 2310, 2466, 2500, 2917, 2929, 3059
HphI	GGTGA	4 cut(s)	221, 735, 875, 2159
Hpy188III	TCNNGA	7 cut(s)	50, 125, 219, 1217, 1550, 2714, 2876
Hpy99I	CGWCG	1 cut(s)	463
HpyAV	CCTTC	6 cut(s)	73, 165, 444, 1489, 1760, 2390
HpyCH4III	ACNGT	7 cut(s)	384, 855, 1499, 1528, 1596, 2297, 2838
Hsp92I	GRCGYC	4 cut(s)	905, 1699, 2104, 2896
HspAI	GCGC	5 cut(s)	161, 905, 2115, 2896, 3083
KasI	GGCGCC	2 cut(s)	904, 2895
KpnI	GGTACC	1 cut(s)	2526
Ksp22I	TGATCA	2 cut(s)	2253, 2382
KspAI	GTTAAC	3 cut(s)	1021, 1921, 2497
KspI	CCGCGG	1 cut(s)	3153
Kzo9I	GATC	9 cut(s)	1120, 1380, 1470, 1533, 1894, 2124, 2253, 2382, 2479
LweI	GCATC	6 cut(s)	327, 355, 439, 471, 788, 2707
MaeI	CTAG	8 cut(s)	194, 900, 996, 1317, 1538, 1556, 2997, 3054
MalI	GATC	9 cut(s)	1122, 1382, 1472, 1535, 1896, 2126, 2255, 2384, 2481
MbiI	CCGCTC	1 cut(s)	2934
MboI	GATC	9 cut(s)	1120, 1380, 1470, 1533, 1894, 2124, 2253, 2382, 2479
MfeI	CAATTG	3 cut(s)	1401, 1431, 1649
MhlI	GDGCHC	2 cut(s)	1054, 1636
MlsI	TGGCCA	3 cut(s)	5, 1399, 1867
MluCI	AATT	9 cut(s)	105, 305, 589, 730, 879, 1401, 1431, 1649, 1884
MluNI	TGGCCA	3 cut(s)	5, 1399, 1867
Mly113I	GGCGCC	2 cut(s)	905, 2896
MlyI	GAGTC	9 cut(s)	1004, 1502, 1837, 2134, 2220, 2501, 2663, 2929, 2995
MmeI	TCCRAC	5 cut(s)	313, 1641, 2080, 2294, 2812
Mox20I	TGGCCA	3 cut(s)	5, 1399, 1867
MscI	TGGCCA	3 cut(s)	5, 1399, 1867
MseI	TTAA	5 cut(s)	1020, 1920, 2166, 2496, 3108
MslI	CAYNNNNRTG	5 cut(s)	423, 525, 1028, 2730, 3006
Msp20I	TGGCCA	3 cut(s)	5, 1399, 1867
MspA1I	CMGCKG	2 cut(s)	514, 3152
MspI	CCGG	9 cut(s)	350, 1040, 2221, 2310, 2466, 2500, 2917, 2929, 3059
MunI	CAATTG	3 cut(s)	1401, 1431, 1649
Mva1269I	GAATGC	5 cut(s)	493, 799, 1782, 2336, 3151
MvaI	CCWGG	8 cut(s)	867, 1002, 1385, 1603, 1695, 2393, 2477, 3137
MvnI	CGCG	1 cut(s)	3152
NarI	GGCGCC	2 cut(s)	905, 2896
NciI	CCSGG	3 cut(s)	2221, 2222, 2466
NcoI	CCATGG	5 cut(s)	74, 619, 688, 1128, 2128
NdeI	CATATG	2 cut(s)	1153, 2727
NdeII	GATC	9 cut(s)	1120, 1380, 1470, 1533, 1894, 2124, 2253, 2382, 2479
NheI	GCTAGC	2 cut(s)	193, 1555
NmeAIII	GCCGAG	1 cut(s)	2813
NmuCI	GTSAC	9 cut(s)	136, 227, 847, 1207, 1267, 2025, 2078, 2973, 3008
NspI	RCATGY	2 cut(s)	323, 571
PaeR7I	CTCGAG	1 cut(s)	2905
PceI	AGGCCT	1 cut(s)	1000
PciI	ACATGT	1 cut(s)	319
PcsI	WCGNNNNNNNCGW	1 cut(s)	1376
PctI	GAATGC	5 cut(s)	493, 799, 1782, 2336, 3151
PfeI	GAWTC	8 cut(s)	121, 202, 487, 1313, 2534, 2689, 3122, 3215
Pfl23II	CGTACG	1 cut(s)	1014
PflFI	GACNNNGTC	2 cut(s)	225, 2288
PflMI	CCANNNNNTGG	1 cut(s)	409
PfoI	TCCNGGA	2 cut(s)	1601, 2391
PinAI	ACCGGT	2 cut(s)	2309, 2499
PleI	GAGTC	9 cut(s)	1004, 1502, 1837, 2134, 2220, 2501, 2663, 2928, 2994
PluTI	GGCGCC	2 cut(s)	908, 2899
PmaCI	CACGTG	1 cut(s)	3089
PmlI	CACGTG	1 cut(s)	3089
PpsI	GAGTC	9 cut(s)	1004, 1502, 1837, 2134, 2220, 2501, 2663, 2928, 2994
Ppu21I	YACGTR	2 cut(s)	2093, 3089
PpuMI	RGGWCCY	2 cut(s)	55, 1643
PscI	ACATGT	1 cut(s)	319
PsiI	TTATAA	1 cut(s)	2051
Psp124BI	GAGCTC	1 cut(s)	1636
Psp1406I	AACGTT	1 cut(s)	739
Psp5II	RGGWCCY	2 cut(s)	55, 1643
Psp6I	CCWGG	8 cut(s)	865, 1000, 1383, 1601, 1693, 2391, 2475, 3135
PspCI	CACGTG	1 cut(s)	3089
PspEI	GGTNACC	1 cut(s)	227
PspFI	CCCAGC	1 cut(s)	354
PspGI	CCWGG	8 cut(s)	865, 1000, 1383, 1601, 1693, 2391, 2475, 3135
PspLI	CGTACG	1 cut(s)	1014
PspPPI	RGGWCCY	2 cut(s)	55, 1643
PspXI	VCTCGAGB	1 cut(s)	2905
PstI	CTGCAG	2 cut(s)	847, 1072
PstNI	CAGNNNCTG	3 cut(s)	29, 514, 2970
PsyI	GACNNNGTC	2 cut(s)	225, 2288
PvuII	CAGCTG	1 cut(s)	514
RsaI	GTAC	9 cut(s)	210, 441, 650, 1016, 1853, 2196, 2415, 2524, 3240
RsaNI	GTAC	9 cut(s)	209, 440, 649, 1015, 1852, 2195, 2414, 2523, 3239
RseI	CAYNNNNRTG	5 cut(s)	423, 525, 1028, 2730, 3006
SacI	GAGCTC	1 cut(s)	1636
SacII	CCGCGG	1 cut(s)	3153
SaqAI	TTAA	5 cut(s)	1020, 1920, 2166, 2496, 3108
Sau3AI	GATC	9 cut(s)	1120, 1380, 1470, 1533, 1894, 2124, 2253, 2382, 2479
SchI	GAGTC	9 cut(s)	1004, 1502, 1837, 2134, 2220, 2501, 2663, 2929, 2995
SduI	GDGCHC	2 cut(s)	1054, 1636
SfaNI	GCATC	6 cut(s)	327, 355, 439, 471, 788, 2707
SfcI	CTRYAG	6 cut(s)	843, 1068, 2911, 2941, 3040, 3126
SfoI	GGCGCC	2 cut(s)	906, 2897
Sfr274I	CTCGAG	1 cut(s)	2905
Sfr303I	CCGCGG	1 cut(s)	3153
SgrBI	CCGCGG	1 cut(s)	3153
SlaI	CTCGAG	1 cut(s)	2905
SmaI	CCCGGG	1 cut(s)	2222
SmiMI	CAYNNNNRTG	5 cut(s)	423, 525, 1028, 2730, 3006
SmlI	CTYRAG	4 cut(s)	831, 2142, 2421, 2905
SmoI	CTYRAG	4 cut(s)	831, 2142, 2421, 2905
Sse9I	AATT	9 cut(s)	105, 305, 589, 730, 879, 1401, 1431, 1649, 1884
SseBI	AGGCCT	1 cut(s)	1000
SspDI	GGCGCC	2 cut(s)	904, 2895
SspI	AATATT	1 cut(s)	2055
SspMI	CTAG	8 cut(s)	194, 900, 996, 1317, 1538, 1556, 2997, 3054
SstI	GAGCTC	1 cut(s)	1636
StuI	AGGCCT	1 cut(s)	1000
StyI	CCWWGG	7 cut(s)	74, 619, 688, 899, 1128, 1188, 2128
TaaI	ACNGT	7 cut(s)	384, 855, 1499, 1528, 1596, 2297, 2838
TaqI	TCGA	6 cut(s)	407, 2229, 2314, 2482, 2906, 2923
TaqII	GACCGA	2 cut(s)	1788, 3027
TasI	AATT	9 cut(s)	105, 305, 589, 730, 879, 1401, 1431, 1649, 1884
TatI	WGTACW	4 cut(s)	439, 648, 1851, 2194
TauI	GCSGC	5 cut(s)	418, 1198, 3152, 3155, 3233
TfiI	GAWTC	8 cut(s)	121, 202, 487, 1313, 2534, 2689, 3122, 3215
Tru1I	TTAA	5 cut(s)	1020, 1920, 2166, 2496, 3108
Tru9I	TTAA	5 cut(s)	1020, 1920, 2166, 2496, 3108
TscAI	CASTG	2 cut(s)	1502, 3252
TseFI	GTSAC	9 cut(s)	136, 227, 847, 1207, 1267, 2025, 2078, 2973, 3008
Tsp45I	GTSAC	9 cut(s)	136, 227, 847, 1207, 1267, 2025, 2078, 2973, 3008
TspGWI	ACGGA	1 cut(s)	1796
TspMI	CCCGGG	1 cut(s)	2220
TspRI	CASTG	2 cut(s)	1502, 3252
Tth111I	GACNNNGTC	2 cut(s)	225, 2288
Van91I	CCANNNNNTGG	1 cut(s)	409
XapI	RAATTY	3 cut(s)	105, 589, 730
XceI	RCATGY	2 cut(s)	323, 571
XhoI	CTCGAG	1 cut(s)	2905
XmaI	CCCGGG	1 cut(s)	2220
XmaJI	CCTAGG	1 cut(s)	899
XspI	CTAG	8 cut(s)	194, 900, 996, 1317, 1538, 1556, 2997, 3054
ZraI	GACGTC	1 cut(s)	2105

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.