Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0008446

Physical Location & Seq

Genomic Coordinates Forward (+)

11390791 .. 11396476

Gene Structure 5686 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1145900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3414 bp

ATGGCATCAGTGGAGCCAGCCGGTCCACGATATGCGCCAGATGATCCTACACTTCCAAATCCGTGGAAGGGTTTGATTGATGGTAGCACGGGTTTGTTATACTATTGGAATCCTGAAACTAACGTTACCCAGTATGAAAAACCGGCTTCTTTGCCTCCGCCCTTGCCAGCAGGCCCGCCTCCTGCTACTACCACACCAAAGCTGGCTGCAATTCCTGTTGCTCATTCAATGCAACCGAACGGAGTGATGCCTCAGGATGGGCAGCAGATAACACAAGCTCCACAACAGCAGGGGTCGCAACTAAACCAGTATTCTCAACCTCATGGACATATAATGGCACAACAGCTGAATCCGACATCATATGCTCAACAGCAGGGATCACAAATGGCTCAACCTGGACATCAACAGAGTTCACAAGTGGGACAGGCCATGCAACATCATGGGCATATGATGCAGCATCTGGGTCAGCAAATGCCTCAGCAACTGGGGCAACACACACCACAGGGTCACGGGTCACAAATGCATCAGTTTGCACATCAGCAGGTGCATTATATCCCTTATCAGCAAAGCATTCCTCCGCAAGGGCAGCAAAGTTCACAACAGCAATCTATGCATAGTGCACAAGGGCAACCACTGTCAAATCAACAAGAATATAAACCTGCATTCTCTCAGAGGGAGGAAGATGATTTTCAACAAAGAAATCAAATTGGTGTTTCTCCATCTCGATTTCAACCTGCGGGTGCCTCATCTGTGCAGAATCTTCCTACTGGGACCAATCCAGTACAAATGCCACAGAGGTCTGTTCATCAAGGGCCACCCCAACAATTTGGCGGCCCTATAGGAAACATGCAACACCCTTCCTCTTTTGGTCACCTGCAGCAACCTGGGGCTGATTTGCCTCACCATCAACATGGTTCTAGGTTCCAAAATCAAATGGACCCGGCAATGATACATGGTCAGCAGTCAAATGTGCCTCCTGTAGGATTAAGAATGGGTCATGAAGATAATTTCCATGGTAGAGGTGGAAATGACTATTACTTCAATAGTAACAATGAAGGGCCACCAGGTCCTCAGCAACCCAAGCTTGCATCTATTCCGATTGCAAGAAGTCAGCAGGAGATGAGTATGAGTGGTGGTCCTCCATTTCAAAATGCTACACCAGGTCATGCCAGTGCACTGAATACCATGCCAGGTCCCATGCATAACGTGTACAGTCATGCAACAGGTGGTCAACCTTTCCCAAATAACTCTTTGGTGAAGCCCCCTTATGCCGGACCTACAAGTGTTACTAGTCTTTCACCTGTTGAAGTTTATCGGCAACAACATGAAGTTACTGCATCGGGTGACAATGTTCCTGCACCGTTCATGACATTTGAAGACACTGGTTTTCCTCCAGAGATATTGAGAGAGATTCATGCTGCTGGCTTCACATCTCCCACACCAATTCAGGCACAAACATGGCCAATTGCTCTTCAAAGTAGGGATATAGTTGCAATTGCGAAAACTGGTTCTGGAAAAACTTTGGGCTATTTGATGCCTGCATTCATTCTTCTTAGACAACGCCGTAATCATTCTCAGAATGGCCCAACAGTCTTGGTTTTGGCCCCAACACGGGAGCTTGCAACGCAGATACAAGACGAGGTCTTGAAATTTGGGCGAGCTTCTAGGGTATCTTGCACGTGCTTGTATGGTGGAGCTCCAAAAGTCCTTCAGCTAAAAGAGTTAGATCGTGGAGCAGATATAGTTGTGGCAACTCCTGGTCGTCTTAATGACATACTGGAAATGAAGAAAATTGATTTTCGGCAAGTTTCTCTACTTGTACTTGATGAAGCAGATCGAATGCTTGATATGGGTTTTGAACCTCAAATCCGTAAAATCGTGGATCAAATACCCCCGCACAGACAAACCCTTATGTACACAGCAACATGGCCCAAAGAAGTAAGAAAAATAGCAAGTGACCTTCTTGTTAATCCTGTACAAGTGAATATTGGCAATGTTGACGAGCTTGCTGCAAACAAGTCTATCACACAGTACGTTGAAGTTGTCCCACAAATGGAGAAGCAAAGGCGCTTGGAGCAGATCCTAAGATCCCAAGAACAAGGTTCTAAGATCATTGTCTTTTGTTCCACCAAGAGGTTGTGCGATCAGATTGCACGTAGTATTGGACGCACCTTTGGGGCTGCTGCAATTCACGGGGACAAGTCTCAAGGTGAGCGAGACTGGGTTTTAAATCAGTTCCGGAGTGGAAAATCCCCAGTATTAGTTGCCACTGACGTTGCTGCTCGTGGGCTTGACATCAAAGATATAAGGGTGGTGGTAAACTATGATTTCCCTACTGGTGTTGAAGACTATGTTCACCGAATTGGAAGAACTGGGAGGGCTGGCGCAACTGGGATGTCCTACACCTTTTTCTCCGAGCAAGACTGGAAATATGCAGCTGATTTGATTAAAGTTCTGGAGGGAGCTAACCAGCAAGTGCCTCCAGAGGTGCGAGAGATTGCTCTACGTGGTGGGGCTGGTTTTGGCAAGGATAGGAATCCGATGAACCGTTTTGATTCTGGTGGGCCTGTTAATGGTGGTACTGGCCGCTGGGATTCTGGCGGCCGTGATGGAATGAGGGATGGGGGTTTTGGTGGTCGCGGCGGTACAAGGGATGGGGGTTTTGGAGGTCGCGGCGGTACAAGGGATGGGGGTTTTGGAGGTCGCGGCGGTACAAGGGATGGGGGTGGTGGCATGAGGGATGGTGGTTTTGGTGGTCGTGGCGGCATGCGAGATGGCTTTGGCGGTCGTGGTGGCTCTAGGGATGGTGCTGTTGGCGGCCGTGGTGGGAGAGGCGATTTCTTTTCTGGGCCTGGGAACAGGGGCCGAGGATTTGGTGGTCCTGGTGGTGGTAATGTTGGTTGGGGTAGAAATGGCAGTGGCCCACAAGATCGGTACAACAGTATGGATGGGCGTGGGGGACGTGGACGGGGACGATTTGATAATAGAGTTGATTTTCCTGACAGGAGCAGAGGCAGAAGCTATAGCCGTAGCCCTGACAGAGTTCGAACATGGGATATCAACAGAAGCAGAAGCCGGAGTCGAAGCCGCAGCCGCAGCCGGAGTAGGAGGAGTCGGAGCTGGACCAGGAGTCGGAGTCGTAGTCGGAGCTGGTCCCGTGGCCGTAGTCGCAGCTACAGTCGTAGCCGAAGTCGTAGTCGGAGCCCCAGTTATGAAAGATATGAGAGGCCGCCACGTGTTTCGAAATTTGATGTGAAGGATCCTGTTCCTGAATCTGGGGCTCCACCTGTACCAGAGATGGTTCCTAAGTCCCCAGACACCAAGCCCATGGGCGAACTGCCAGTGGTTGAGGAAACTGAGCCAGTGCATACAAAGGGTGTTGGAGGAGACCCTTCTCATCATCCAGCGACTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005773 GO:0006139 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009987 GO:0010501 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031974 GO:0031981 GO:0034641 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0070013 GO:0070035 GO:0071704 GO:0090304 GO:0140098 GO:1901360

KEGG Pathways

Metabolic & Signaling

ko03040 ko05202 ko05205 map03040 map05202 map05205

Pfam Domains

Protein Families

DEAD Helicase_C WW

Protein Analysis

1138

Amino Acids

123.88

Weight (kDa)

9.9

Isoelectric Point (pI)

61.33

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WW	PF00397	18 - 48	2.2e-07	WW domain
DEAD	PF00270	480 - 649	6.9e-49	DEAD/DEAH box helicase
ResIII	PF04851	492 - 643	6.7e-10	Type III restriction enzyme, res subunit
Helicase_C	PF00271	687 - 795	1.1e-33	Helicase conserved C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0008446)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G06480 AT3G06480
fragaria_vesca	FvH4_4g21040
malus_domestica	MD13G1145900.v1.1 MD16G1139800.v1.1 MD16G1139900.v1.1
prunus_persica	Prupe.1G109600_v2.0.a1
pyrus_communis	pycom13g12540 pycom16g12400
rosa_chinensis	RchiOBHm_Chr2g0174651 RchiOBHm_Chr4g0426571
rosa_laevigata	RLG00000007289
rosa_multiflora	Rmu_co8268839.1_g000001 Rmu_sc0001821.1_g000009 Rmu_sc0007189.1_g000006
rosa_roxburghii	Rroxscaffold_5G00368580
rosa_rugosa	Rorug04G0213500
rosa_samantha	Rh4AG268700 Rh4BG275000 Rh4CG289300 Rh4DG271800
rosa_wichuraiana	Rw2G054030 Rw4G023310

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	532, 882
Acc36I	ACCTGC	4 cut(s)	532, 667, 742, 882
AccB1I	GGYRCC	1 cut(s)	740
AccII	CGCG	3 cut(s)	2640, 2673, 2706
AccIII	TCCGGA	1 cut(s)	2238
AclI	AACGTT	1 cut(s)	123
AclWI	GGATC	7 cut(s)	38, 385, 1890, 2074, 2082, 3254, 3267
AcoI	YGGCCR	5 cut(s)	1460, 2584, 2602, 2818, 3160
AcsI	RAATTY	2 cut(s)	1649, 3245
AcuI	CTGAAG	1 cut(s)	1694
AcvI	CACGTG	2 cut(s)	1680, 3236
AflIII	ACRYGT	2 cut(s)	1206, 3235
AhdI	GACNNNNNGTC	1 cut(s)	3129
AhlI	ACTAGT	1 cut(s)	1289
AjiI	CACGTC	1 cut(s)	2963
AjnI	CCWGG	9 cut(s)	394, 883, 1063, 1159, 1189, 1756, 2851, 2881, 3125
AloI	GAACNNNNNNTCC	2 cut(s)	1851, 1883
Alw21I	GWGCWC	3 cut(s)	622, 1177, 1699
Alw26I	GTCTC	3 cut(s)	2208, 2211, 3381
Alw44I	GTGCAC	2 cut(s)	618, 1173
AlwI	GGATC	7 cut(s)	38, 385, 1890, 2074, 2082, 3254, 3267
AlwNI	CAGNNNCTG	2 cut(s)	460, 484
Aor13HI	TCCGGA	1 cut(s)	2238
ApaLI	GTGCAC	2 cut(s)	618, 1173
ApoI	RAATTY	2 cut(s)	1649, 3245
ArsI	GACNNNNNNTTYG	2 cut(s)	3181, 3213
AspLEI	GCGC	3 cut(s)	37, 2070, 2387
AsuC2I	CCSGG	1 cut(s)	941
AsuHPI	GGTGA	7 cut(s)	863, 893, 1267, 1290, 1355, 2222, 2348
AsuII	TTCGAA	2 cut(s)	3046, 3242
AxyI	CCTNAGG	1 cut(s)	252
BaeGI	GKGCMC	2 cut(s)	622, 1177
BaeI	ACNNNNGTAYC	2 cut(s)	2926, 2959
BalI	TGGCCA	1 cut(s)	1462
BamHI	GGATCC	1 cut(s)	3259
BanI	GGYRCC	1 cut(s)	740
BanII	GRGCYC	3 cut(s)	1699, 3206, 3283
BauI	CACGAG	1 cut(s)	2283
BbrPI	CACGTG	2 cut(s)	1680, 3236
BbsI	GAAGAC	2 cut(s)	1383, 2352
Bbv12I	GWGCWC	3 cut(s)	622, 1177, 1699
BbvCI	CCTCAGC	2 cut(s)	477, 1071
BceAI	ACGGC	5 cut(s)	1548, 2589, 2805, 3012, 3147
BcgI	CGANNNNNNTGC	4 cut(s)	1991, 2025, 2132, 2166
BciT130I	CCWGG	9 cut(s)	396, 885, 1065, 1161, 1191, 1758, 2853, 2883, 3127
BcnI	CCSGG	1 cut(s)	941
BcoDI	GTCTC	3 cut(s)	2208, 2211, 3381
BcuI	ACTAGT	1 cut(s)	1289
BfaI	CTAG	4 cut(s)	918, 1290, 1665, 2799
BfmI	CTRYAG	5 cut(s)	837, 875, 978, 3022, 3175
BfoI	RGCGCY	1 cut(s)	2071
BfuAI	ACCTGC	4 cut(s)	532, 667, 742, 882
BmeRI	GACNNNNNGTC	1 cut(s)	3129
BmgBI	CACGTC	1 cut(s)	2963
BmrI	ACTGGG	8 cut(s)	124, 494, 777, 2230, 2249, 2382, 2400, 3201
BmsI	GCATC	8 cut(s)	14, 237, 441, 466, 532, 1097, 1346, 1524
BmuI	ACTGGG	8 cut(s)	124, 494, 777, 2230, 2249, 2382, 2400, 3201
BpiI	GAAGAC	2 cut(s)	1383, 2352
BpmI	CTGGAG	3 cut(s)	1377, 2466, 2477
Bpu10I	CCTNAGC	2 cut(s)	477, 1071
Bpu14I	TTCGAA	2 cut(s)	3046, 3242
BpuEI	CTTGAG	1 cut(s)	2190
BpuMI	CCSGG	1 cut(s)	941
BsaAI	YACGTR	4 cut(s)	1680, 2156, 2507, 3236
BsaBI	GATNNNNATC	1 cut(s)	2535
BsaI	GGTCTC	1 cut(s)	3381
BsaJI	CCNNGG	8 cut(s)	62, 884, 1012, 2821, 2852, 2866, 3157, 3327
BsaWI	WCCGGW	1 cut(s)	2238
BsaXI	ACNNNNNCTCC	8 cut(s)	262, 292, 3121, 3127, 3151, 3157, 3193, 3223
Bse118I	RCCGGY	2 cut(s)	20, 142
Bse21I	CCTNAGG	1 cut(s)	252
Bse3DI	GCAATG	2 cut(s)	951, 1999
Bse8I	GATNNNNATC	1 cut(s)	2535
BseAI	TCCGGA	1 cut(s)	2238
BseBI	CCWGG	9 cut(s)	396, 885, 1065, 1161, 1191, 1758, 2853, 2883, 3127
BseDI	CCNNGG	8 cut(s)	62, 884, 1012, 2821, 2852, 2866, 3157, 3327
BseJI	GATNNNNATC	1 cut(s)	2535
BseMI	GCAATG	2 cut(s)	951, 1999
BseMII	CTCAG	6 cut(s)	266, 491, 683, 1085, 1589, 3348
BseRI	GAGGAG	2 cut(s)	3124, 3399
BseSI	GKGCMC	2 cut(s)	622, 1177
BseX3I	CGGCCG	2 cut(s)	2602, 2818
BseYI	CCCAGC	1 cut(s)	2589
BsgI	GTGCAG	2 cut(s)	773, 1341
Bsh1236I	CGCG	3 cut(s)	2640, 2673, 2706
Bsh1285I	CGRYCG	3 cut(s)	2605, 2788, 2821
BshNI	GGYRCC	1 cut(s)	740
BsiEI	CGRYCG	3 cut(s)	2605, 2788, 2821
BsiHKAI	GWGCWC	3 cut(s)	622, 1177, 1699
BsiSI	CCGG	7 cut(s)	21, 143, 941, 1272, 2239, 3076, 3100
BslFI	GGGAC	9 cut(s)	435, 784, 1179, 2030, 2210, 2973, 2985, 3139, 3295
BsmAI	GTCTC	3 cut(s)	2208, 2211, 3381
BsmFI	GGGAC	9 cut(s)	435, 784, 1179, 2030, 2210, 2973, 2985, 3139, 3295
BsmI	GAATGC	4 cut(s)	570, 662, 1541, 1845
Bso31I	GGTCTC	1 cut(s)	3381
Bsp119I	TTCGAA	2 cut(s)	3046, 3242
Bsp1286I	GDGCHC	5 cut(s)	622, 1177, 1699, 3206, 3283
Bsp13I	TCCGGA	1 cut(s)	2238
Bsp1407I	TGTACA	3 cut(s)	1209, 1914, 1975
Bsp19I	CCATGG	2 cut(s)	1012, 3327
BspCNI	CTCAG	6 cut(s)	265, 490, 682, 1084, 1588, 3349
BspEI	TCCGGA	1 cut(s)	2238
BspFNI	CGCG	3 cut(s)	2640, 2673, 2706
BspHI	TCATGA	2 cut(s)	997, 1365
BspMAI	CTGCAG	1 cut(s)	879
BspMI	ACCTGC	4 cut(s)	532, 667, 742, 882
BspPI	GGATC	7 cut(s)	38, 385, 1890, 2074, 2082, 3254, 3267
BspQI	GCTCTTC	1 cut(s)	1476
BspT104I	TTCGAA	2 cut(s)	3046, 3242
BspT107I	GGYRCC	1 cut(s)	740
BspTNI	GGTCTC	1 cut(s)	3381
BsrDI	GCAATG	2 cut(s)	951, 1999
BsrFI	RCCGGY	2 cut(s)	20, 142
BsrGI	TGTACA	3 cut(s)	1209, 1914, 1975
BssAI	RCCGGY	2 cut(s)	20, 142
BssECI	CCNNGG	8 cut(s)	62, 884, 1012, 2821, 2852, 2866, 3157, 3327
BssSI	CACGAG	1 cut(s)	2283
BssT1I	CCWWGG	2 cut(s)	1012, 3327
Bst2BI	CACGAG	1 cut(s)	2283
Bst2UI	CCWGG	9 cut(s)	396, 885, 1065, 1161, 1191, 1758, 2853, 2883, 3127
Bst4CI	ACNGT	8 cut(s)	636, 1214, 1362, 1591, 2031, 2549, 2942, 3179
Bst6I	CTCTTC	1 cut(s)	1476
BstAPI	GCANNNNNTGC	2 cut(s)	451, 610
BstAUI	TGTACA	3 cut(s)	1209, 1914, 1975
BstBAI	YACGTR	4 cut(s)	1680, 2156, 2507, 3236
BstBI	TTCGAA	2 cut(s)	3046, 3242
BstDSI	CCRYGG	5 cut(s)	62, 1012, 2821, 3157, 3327
BstEII	GGTNACC	1 cut(s)	869
BstENI	CCTNNNNNAGG	2 cut(s)	579, 978
BstFNI	CGCG	3 cut(s)	2640, 2673, 2706
BstH2I	RGCGCY	1 cut(s)	2071
BstHHI	GCGC	3 cut(s)	37, 2070, 2387
BstMAI	GTCTC	3 cut(s)	2208, 2211, 3381
BstMCI	CGRYCG	3 cut(s)	2605, 2788, 2821
BstNI	CCWGG	9 cut(s)	396, 885, 1065, 1161, 1191, 1758, 2853, 2883, 3127
BstNSI	RCATGY	2 cut(s)	850, 2770
BstPI	GGTNACC	1 cut(s)	869
BstSFI	CTRYAG	5 cut(s)	837, 875, 978, 3022, 3175
BstSLI	GKGCMC	2 cut(s)	622, 1177
BstUI	CGCG	3 cut(s)	2640, 2673, 2706
BstV2I	GAAGAC	2 cut(s)	1383, 2352
BstX2I	RGATCY	3 cut(s)	2079, 2087, 3259
BstXI	CCANNNNNNTGG	4 cut(s)	63, 827, 911, 3328
BstYI	RGATCY	3 cut(s)	2079, 2087, 3259
BstZI	CGGCCG	2 cut(s)	2602, 2818
Bsu36I	CCTNAGG	1 cut(s)	252
BtgI	CCRYGG	5 cut(s)	62, 1012, 2821, 3157, 3327
BtrI	CACGTC	1 cut(s)	2963
BtsI	GCAGTG	1 cut(s)	2923
BtsIMutI	CAGTG	9 cut(s)	15, 632, 1175, 1177, 1380, 2268, 2923, 3348, 3369
BveI	ACCTGC	4 cut(s)	532, 667, 742, 882
CaiI	CAGNNNCTG	2 cut(s)	460, 484
CciI	TCATGA	2 cut(s)	997, 1365
CfoI	GCGC	3 cut(s)	37, 2070, 2387
Cfr10I	RCCGGY	2 cut(s)	20, 142
CseI	GACGC	1 cut(s)	2175
CsiI	ACCWGGT	2 cut(s)	1063, 1159
CspCI	CAANNNNNGTGG	2 cut(s)	2257, 2292
DraI	TTTAAA	1 cut(s)	2229
DriI	GACNNNNNGTC	1 cut(s)	3129
EaeI	YGGCCR	5 cut(s)	1460, 2584, 2602, 2818, 3160
EagI	CGGCCG	2 cut(s)	2602, 2818
Eam1104I	CTCTTC	1 cut(s)	1476
Eam1105I	GACNNNNNGTC	1 cut(s)	3129
EarI	CTCTTC	1 cut(s)	1476
EciI	GGCGGA	1 cut(s)	147
Ecl136II	GAGCTC	1 cut(s)	1697
EclXI	CGGCCG	2 cut(s)	2602, 2818
Eco130I	CCWWGG	2 cut(s)	1012, 3327
Eco24I	GRGCYC	3 cut(s)	1699, 3206, 3283
Eco31I	GGTCTC	1 cut(s)	3381
Eco32I	GATATC	1 cut(s)	3058
Eco52I	CGGCCG	2 cut(s)	2602, 2818
Eco53kI	GAGCTC	1 cut(s)	1697
Eco57I	CTGAAG	1 cut(s)	1694
Eco72I	CACGTG	2 cut(s)	1680, 3236
Eco81I	CCTNAGG	1 cut(s)	252
Eco91I	GGTNACC	1 cut(s)	869
EcoICRI	GAGCTC	1 cut(s)	1697
EcoNI	CCTNNNNNAGG	2 cut(s)	579, 978
EcoO109I	RGGNCCY	2 cut(s)	1067, 1193
EcoO65I	GGTNACC	1 cut(s)	869
EcoRII	CCWGG	9 cut(s)	394, 883, 1063, 1159, 1189, 1756, 2851, 2881, 3125
EcoRV	GATATC	1 cut(s)	3058
EcoT14I	CCWWGG	2 cut(s)	1012, 3327
EcoT22I	ATGCAT	3 cut(s)	525, 615, 1203
EcoT38I	GRGCYC	3 cut(s)	1699, 3206, 3283
ErhI	CCWWGG	2 cut(s)	1012, 3327
FaqI	GGGAC	9 cut(s)	435, 784, 1179, 2030, 2210, 2973, 2985, 3139, 3295
FauI	CCCGC	3 cut(s)	183, 730, 1902
FauNDI	CATATG	2 cut(s)	361, 447
FriOI	GRGCYC	3 cut(s)	1699, 3206, 3283
FspBI	CTAG	4 cut(s)	918, 1290, 1665, 2799
GlaI	GCGC	3 cut(s)	36, 2069, 2386
GsaI	CCCAGC	1 cut(s)	2593
GsuI	CTGGAG	3 cut(s)	1377, 2466, 2477
HaeII	RGCGCY	1 cut(s)	2071
HapII	CCGG	7 cut(s)	21, 143, 941, 1272, 2239, 3076, 3100
HgaI	GACGC	1 cut(s)	2175
HhaI	GCGC	3 cut(s)	37, 2070, 2387
Hin6I	GCGC	3 cut(s)	35, 2068, 2385
HinP1I	GCGC	3 cut(s)	35, 2068, 2385
HincII	GTYRAC	2 cut(s)	1232, 1999
HindII	GTYRAC	2 cut(s)	1232, 1999
HindIII	AAGCTT	1 cut(s)	1082
HpaII	CCGG	7 cut(s)	21, 143, 941, 1272, 2239, 3076, 3100
HphI	GGTGA	7 cut(s)	863, 893, 1267, 1290, 1355, 2222, 2348
HpyAV	CCTTC	7 cut(s)	61, 867, 1049, 1718, 1970, 3250, 3402
HpyCH4III	ACNGT	8 cut(s)	636, 1214, 1362, 1591, 2031, 2549, 2942, 3179
HpyCH4IV	ACGT	9 cut(s)	123, 1206, 1679, 2034, 2155, 2274, 2506, 2962, 3235
HpySE526I	ACGT	9 cut(s)	123, 1206, 1679, 2034, 2155, 2274, 2506, 2962, 3235
HspAI	GCGC	3 cut(s)	35, 2068, 2385
Kpn2I	TCCGGA	1 cut(s)	2238
LguI	GCTCTTC	1 cut(s)	1476
LweI	GCATC	8 cut(s)	14, 237, 441, 466, 532, 1097, 1346, 1524
MabI	ACCWGGT	2 cut(s)	1063, 1159
MaeI	CTAG	4 cut(s)	918, 1290, 1665, 2799
MaeII	ACGT	9 cut(s)	123, 1206, 1679, 2034, 2155, 2274, 2506, 2962, 3235
MaeIII	GTNAC	9 cut(s)	124, 506, 513, 869, 1046, 1285, 1330, 1343, 1955
MboII	GAAGA	9 cut(s)	692, 752, 1013, 1388, 1463, 1541, 1798, 2357, 2379
MfeI	CAATTG	2 cut(s)	1464, 1494
MflI	RGATCY	3 cut(s)	2079, 2087, 3259
MhlI	GDGCHC	5 cut(s)	622, 1177, 1699, 3206, 3283
MlsI	TGGCCA	1 cut(s)	1462
MluNI	TGGCCA	1 cut(s)	1462
MlyI	GAGTC	4 cut(s)	3088, 3121, 3139, 3145
MmeI	TCCRAC	6 cut(s)	377, 3095, 3113, 3125, 3179, 3361
Mox20I	TGGCCA	1 cut(s)	1462
Mph1103I	ATGCAT	3 cut(s)	525, 615, 1203
MroI	TCCGGA	1 cut(s)	2238
MscI	TGGCCA	1 cut(s)	1462
MseI	TTAA	7 cut(s)	986, 1767, 1968, 2228, 2448, 2571, 3412
MslI	CAYNNNNRTG	3 cut(s)	909, 1170, 1456
Msp20I	TGGCCA	1 cut(s)	1462
MspA1I	CMGCKG	3 cut(s)	346, 2438, 2589
MspI	CCGG	7 cut(s)	21, 143, 941, 1272, 2239, 3076, 3100
MunI	CAATTG	2 cut(s)	1464, 1494
Mva1269I	GAATGC	4 cut(s)	570, 662, 1541, 1845
MvaI	CCWGG	9 cut(s)	396, 885, 1065, 1161, 1191, 1758, 2853, 2883, 3127
MvnI	CGCG	3 cut(s)	2640, 2673, 2706
NciI	CCSGG	1 cut(s)	941
NcoI	CCATGG	2 cut(s)	1012, 3327
NdeI	CATATG	2 cut(s)	361, 447
NmeAIII	GCCGAG	1 cut(s)	2891
NmuCI	GTSAC	5 cut(s)	506, 513, 869, 1343, 1955
NsiI	ATGCAT	3 cut(s)	525, 615, 1203
NspI	RCATGY	2 cut(s)	850, 2770
NspV	TTCGAA	2 cut(s)	3046, 3242
PaeI	GCATGC	1 cut(s)	2770
PagI	TCATGA	2 cut(s)	997, 1365
PaqCI	CACCTGC	2 cut(s)	532, 882
PciSI	GCTCTTC	1 cut(s)	1476
PctI	GAATGC	4 cut(s)	570, 662, 1541, 1845
PfeI	GAWTC	8 cut(s)	109, 349, 757, 1411, 2536, 2555, 2594, 3272
PflFI	GACNNNGTC	1 cut(s)	1640
PleI	GAGTC	4 cut(s)	3087, 3120, 3138, 3144
PmaCI	CACGTG	2 cut(s)	1680, 3236
PmlI	CACGTG	2 cut(s)	1680, 3236
PpsI	GAGTC	4 cut(s)	3087, 3120, 3138, 3144
Ppu21I	YACGTR	4 cut(s)	1680, 2156, 2507, 3236
PpuMI	RGGWCCY	2 cut(s)	1067, 1193
Psp124BI	GAGCTC	1 cut(s)	1699
Psp1406I	AACGTT	1 cut(s)	123
Psp5II	RGGWCCY	2 cut(s)	1067, 1193
Psp6I	CCWGG	9 cut(s)	394, 883, 1063, 1159, 1189, 1756, 2851, 2881, 3125
PspCI	CACGTG	2 cut(s)	1680, 3236
PspEI	GGTNACC	1 cut(s)	869
PspFI	CCCAGC	1 cut(s)	2589
PspGI	CCWGG	9 cut(s)	394, 883, 1063, 1159, 1189, 1756, 2851, 2881, 3125
PspPPI	RGGWCCY	2 cut(s)	1067, 1193
PstI	CTGCAG	1 cut(s)	879
PstNI	CAGNNNCTG	2 cut(s)	460, 484
PsuI	RGATCY	3 cut(s)	2079, 2087, 3259
PsyI	GACNNNGTC	1 cut(s)	1640
PvuII	CAGCTG	2 cut(s)	346, 2438
RseI	CAYNNNNRTG	3 cut(s)	909, 1170, 1456
SacI	GAGCTC	1 cut(s)	1699
SapI	GCTCTTC	1 cut(s)	1476
SaqAI	TTAA	7 cut(s)	986, 1767, 1968, 2228, 2448, 2571, 3412
SchI	GAGTC	4 cut(s)	3088, 3121, 3139, 3145
SduI	GDGCHC	5 cut(s)	622, 1177, 1699, 3206, 3283
SexAI	ACCWGGT	2 cut(s)	1063, 1159
SfaNI	GCATC	8 cut(s)	14, 237, 441, 466, 532, 1097, 1346, 1524
SfcI	CTRYAG	5 cut(s)	837, 875, 978, 3022, 3175
SfuI	TTCGAA	2 cut(s)	3046, 3242
SmiMI	CAYNNNNRTG	3 cut(s)	909, 1170, 1456
SmlI	CTYRAG	1 cut(s)	2205
SmoI	CTYRAG	1 cut(s)	2205
SpeI	ACTAGT	1 cut(s)	1289
SphI	GCATGC	1 cut(s)	2770
SspI	AATATT	1 cut(s)	1987
SspMI	CTAG	4 cut(s)	918, 1290, 1665, 2799
SstI	GAGCTC	1 cut(s)	1699
StyI	CCWWGG	2 cut(s)	1012, 3327
TaaI	ACNGT	8 cut(s)	636, 1214, 1362, 1591, 2031, 2549, 2942, 3179
TaiI	ACGT	9 cut(s)	126, 1209, 1682, 2037, 2158, 2277, 2509, 2965, 3238
TaqI	TCGA	5 cut(s)	724, 1837, 3046, 3082, 3242
TatI	WGTACW	5 cut(s)	781, 1209, 1819, 1914, 1975
TfiI	GAWTC	8 cut(s)	109, 349, 757, 1411, 2536, 2555, 2594, 3272
Tru1I	TTAA	7 cut(s)	986, 1767, 1968, 2228, 2448, 2571, 3412
Tru9I	TTAA	7 cut(s)	986, 1767, 1968, 2228, 2448, 2571, 3412
TscAI	CASTG	9 cut(s)	15, 639, 1177, 1182, 1387, 2275, 2923, 3348, 3369
TseFI	GTSAC	5 cut(s)	506, 513, 869, 1343, 1955
Tsp45I	GTSAC	5 cut(s)	506, 513, 869, 1343, 1955
TspGWI	ACGGA	3 cut(s)	51, 255, 1859
TspRI	CASTG	9 cut(s)	15, 639, 1177, 1182, 1387, 2275, 2923, 3348, 3369
Tth111I	GACNNNGTC	1 cut(s)	1640
VneI	GTGCAC	2 cut(s)	618, 1173
XagI	CCTNNNNNAGG	2 cut(s)	579, 978
XapI	RAATTY	2 cut(s)	1649, 3245
XceI	RCATGY	2 cut(s)	850, 2770
XcmI	CCANNNNNNNNNTGG	2 cut(s)	199, 1019
XspI	CTAG	4 cut(s)	918, 1290, 1665, 2799
Zsp2I	ATGCAT	3 cut(s)	525, 615, 1203

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.