Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0008446

Physical Location & Seq

Genomic Coordinates Forward (+)

10759854 .. 10763538

Gene Structure 3685 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1139900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2034 bp

ATGGCTCCACCTGTTATGTCTTTATGCCCTCTTTTGTTGCCTCAAGTTGCTTGTATTCATGCTGCTGGCTTCGCATCTCCCACGCCAATTCAGGCACAAACATGGCCAATTGCTCTTCAAAGTAGGGATATAGTTGCAATTGCAAAAACTGGTTCCGGAAAAACTTTGGGCTATTTGATGCCTGCATTCATTCTTCTTAGACAACGCCATAATAATTCTCAGAATGGCCCAACAGTCCTGGTTTTGGCCCCAACACGGGAGCTTGCAACACAGATACAAGATGAGGTTTTGAAATTTGGGCGAGCTTCTAGGGTATCTTGCACGTGCCTGTATGGTGGAGCTCTGAAGGGCCCTCAGCTAAAAGAGTTAGATCGTGGAGCAGACATAGTGGTGGCAACTCCTGGTCGTCTTAATGACATACTGGAAATGAAGAAAATTGATTTTCGGCAAGTTTCTCTACTTGTACTTGATGAAGCAGATCGAATGCTTGACATGGGTTTTGAACCTCAAATCCGTAAAATCGTGGATCAAATACCCCCGCACAGACAAACTCTTATGTACACAGCAACATGGCCCAAAGAAGTAAGAAAGATAGCAAGTGATCTTCTTGTTAATCCTGTACAAGTGAATATTGGCAGCGTTAATGAGCTTGCTGCAAACAAGTCTATCACACAGTACGTTGAAGTTGTCCCACAAATGGAGAAGCAACGGCGTTTGGAGCAGATCCTGAGATCCCAAGAACAAGGTTCTAAGATCATTGTCTTTTGTTCCACCAAGAGGTTGTGTGATCAGATTGCACGTAGTATTGGACGCACTTTTGGGGCTGCTGCAATTCACGGGGACAAGTCTCAAGGTGAGCGAGACTGGGTTTTAAATCAGTTCCGGAGTGGAAAATCCCCAGTATTAGTTGCCACTGACGTTGCTGCTCGTGGGCTTGACATCAAAGATATAAGGGTGGTGGTAAACTATGATTTCCCTACTGGTGTTGAAGACTATGTTCACCGAATTGGAAGAACTGGGAGGGCTGGCGCAACGGGGGTGTCATACACCTTTTTCTCTGAGCAAGACTGGAAATATGCAGCTGATTTGATTAAAGTTCTGGAGGGAGCTAACCAGCAAGTGCCTCCAGAGGTGCGAGAGATTGCTCTACGTGGTGGGCCTGGTTTTGGCAAGGATAGGAATCCAATGAACCGTTTTGATTCTGGTGGGCCTGTTAATGGTGCTGCTGGCCGTTGGGATTCCGGCGGCCGTGATGGAATTAGGGATGGGGGTTTTGGTGGTCGTGGCAGTATGAGGGATGGGGGCTTTGGAGGTCGTGGTGGTTTTGGTGGTCGTGGCGGCATGCGAGATGGCAACTTTGGCGGTCGTGGCAGCATGCGAGATGGCAACTTTGGCGGTCGTGGGGGCCCTAGGGACAGTGCTGGTAGCGGCCGTGGTGGGAGAGGCGATTTCTTTTCTGGGCCTGGAAACAGGGGGCGAGGATTTGGTGGTCCTGGTGGTGGTAATGTTGGTCGGGGTAGAAATGGCAGTGGCCCACAAGATCGGTACAACAGTATGGATGGCCGTGGGGGACGTGGACGGGGACGATTTGATAATAGAGTTGATTTTCCTGACAGGAGCAGAGGCAGGAACTATAGCCGTAGCCCTGACAGAGTTCGAACATGGGATATTAACAGAAGCAGAAGCCGGAGTCGAAGCCGCAGCATTAGCCGGAGTAGGAGTCGGAGTCGTAGCCGGAGCTGGTCCCGTGGCCGTAGCCGCAGCTACAGCCATAGCCGAAGTCATAGTCGCAACCGTAGTCGGAGCCCCAGTTATGAAAGATATGAGAGGCCGCCACGCGTGTCAAAATTTGATATGAAGGATCCTGTTCCCGAATCTGTGGTTCCACCTGTACCAGAGATGGTTCCTACGTCCCCAGAGACACAAGTCAATGTATATCCAGACACCAAGCCGAATCTGCCAGTGGTTGAGCAAACGGGGCTAGTGGATACACAGGGCGGAGGAGGAGACCCTTCCCATCACTCATTGATTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005773 GO:0006139 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009987 GO:0010501 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031974 GO:0031981 GO:0034641 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0070013 GO:0070035 GO:0071704 GO:0090304 GO:0140098 GO:1901360

KEGG Pathways

Metabolic & Signaling

ko03040 ko05202 ko05205 map03040 map05202 map05205

Pfam Domains

Protein Families

DEAD Helicase_C WW

Protein Analysis

678

Amino Acids

73.91

Weight (kDa)

10.44

Isoelectric Point (pI)

51.19

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	28 - 197	5.3e-49	DEAD/DEAH box helicase
ResIII	PF04851	40 - 191	6.8e-11	Type III restriction enzyme, res subunit
Helicase_C	PF00271	235 - 343	5e-34	Helicase conserved C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008446)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G06480 AT3G06480
fragaria_vesca	FvH4_4g21040
malus_domestica	MD13G1145900.v1.1 MD16G1139800.v1.1 MD16G1139900.v1.1
prunus_persica	Prupe.1G109600_v2.0.a1
pyrus_communis	pycom13g12540 pycom16g12400
rosa_chinensis	RchiOBHm_Chr2g0174651 RchiOBHm_Chr4g0426571
rosa_laevigata	RLG00000007289
rosa_multiflora	Rmu_co8268839.1_g000001 Rmu_sc0001821.1_g000009 Rmu_sc0007189.1_g000006
rosa_roxburghii	Rroxscaffold_5G00368580
rosa_rugosa	Rorug04G0213500
rosa_samantha	Rh4AG268700 Rh4BG275000 Rh4CG289300 Rh4DG271800
rosa_wichuraiana	Rw2G054030 Rw4G023310

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccII	CGCG	1 cut(s)	1839
AccIII	TCCGGA	2 cut(s)	155, 882
AclWI	GGATC	5 cut(s)	534, 718, 726, 1856, 1869
AcoI	YGGCCR	6 cut(s)	104, 1228, 1246, 1429, 1561, 1750
AcsI	RAATTY	2 cut(s)	293, 1847
AcuI	CTGAAG	1 cut(s)	365
AcvI	CACGTG	1 cut(s)	324
AfaI	GTAC	6 cut(s)	465, 560, 621, 677, 1547, 1893
AfiI	CCNNNNNNNGG	8 cut(s)	244, 255, 256, 697, 777, 1166, 1217, 1499
AflIII	ACRYGT	1 cut(s)	1837
AgsI	TTSAA	5 cut(s)	119, 292, 503, 683, 989
AjiI	CACGTC	1 cut(s)	1574
AjnI	CCWGG	5 cut(s)	237, 400, 1159, 1462, 1492
AloI	GAACNNNNNNTCC	2 cut(s)	495, 527
AluBI	AGCT	9 cut(s)	262, 305, 341, 358, 649, 1082, 1109, 1740, 1764
AluI	AGCT	9 cut(s)	262, 305, 341, 358, 649, 1082, 1109, 1740, 1764
Alw21I	GWGCWC	1 cut(s)	343
Alw26I	GTCTC	4 cut(s)	852, 855, 1913, 2001
AlwI	GGATC	5 cut(s)	534, 718, 726, 1856, 1869
AlwNI	CAGNNNCTG	1 cut(s)	727
Aor13HI	TCCGGA	2 cut(s)	155, 882
ApaI	GGGCCC	2 cut(s)	353, 1409
ApoI	RAATTY	2 cut(s)	293, 1847
ArsI	GACNNNNNNTTYG	4 cut(s)	482, 514, 1771, 1803
AspA2I	CCTAGG	1 cut(s)	1409
AspLEI	GCGC	1 cut(s)	1031
AsuHPI	GGTGA	2 cut(s)	866, 992
AsuII	TTCGAA	1 cut(s)	1657
AvaII	GGWCC	2 cut(s)	1490, 1743
AvrII	CCTAGG	1 cut(s)	1409
BaeGI	GKGCMC	2 cut(s)	353, 1409
BaeI	ACNNNNGTAYC	2 cut(s)	1537, 1570
BalI	TGGCCA	1 cut(s)	106
BamHI	GGATCC	1 cut(s)	1861
BanII	GRGCYC	4 cut(s)	343, 353, 1409, 1808
BauI	CACGAG	1 cut(s)	927
BbrPI	CACGTG	1 cut(s)	324
BbsI	GAAGAC	1 cut(s)	996
Bbv12I	GWGCWC	1 cut(s)	343
BbvCI	CCTCAGC	1 cut(s)	354
BccI	CCATC	8 cut(s)	1247, 1259, 1292, 1343, 1376, 1553, 1894, 2025
BceAI	ACGGC	7 cut(s)	725, 1215, 1233, 1416, 1548, 1623, 1737
BciT130I	CCWGG	5 cut(s)	239, 402, 1161, 1464, 1494
BciVI	GTATCC	1 cut(s)	1981
BclI	TGATCA	1 cut(s)	787
BcoDI	GTCTC	4 cut(s)	852, 855, 1913, 2001
BfaI	CTAG	3 cut(s)	309, 1410, 1982
BfmI	CTRYAG	2 cut(s)	1633, 1765
BfuI	GTATCC	1 cut(s)	1981
BlnI	CCTAGG	1 cut(s)	1409
Bme1390I	CCNGG	5 cut(s)	239, 402, 1161, 1464, 1494
Bme18I	GGWCC	2 cut(s)	1490, 1743
BmgBI	CACGTC	1 cut(s)	1574
BmrFI	CCNGG	5 cut(s)	239, 402, 1161, 1464, 1494
BmrI	ACTGGG	4 cut(s)	874, 893, 1026, 1803
BmsI	GCATC	2 cut(s)	83, 168
BmuI	ACTGGG	4 cut(s)	874, 893, 1026, 1803
BoxI	GACNNNNGTC	1 cut(s)	1925
BpiI	GAAGAC	1 cut(s)	996
BpmI	CTGGAG	2 cut(s)	1110, 1121
Bpu10I	CCTNAGC	1 cut(s)	354
Bpu14I	TTCGAA	1 cut(s)	1657
BpuEI	CTTGAG	2 cut(s)	27, 834
BsaAI	YACGTR	3 cut(s)	324, 800, 1151
BsaBI	GATNNNNATC	1 cut(s)	1179
BsaI	GGTCTC	1 cut(s)	2001
BsaJI	CCNNGG	4 cut(s)	1409, 1432, 1564, 1747
BsaWI	WCCGGW	2 cut(s)	155, 882
BsaXI	ACNNNNNCTCC	6 cut(s)	1705, 1711, 1735, 1741, 1795, 1825
Bsc4I	CCNNNNNNNGG	8 cut(s)	244, 255, 256, 697, 777, 1166, 1217, 1499
Bse1I	ACTGG	9 cut(s)	154, 426, 869, 899, 985, 1021, 1073, 1809, 1961
Bse8I	GATNNNNATC	1 cut(s)	1179
BseAI	TCCGGA	2 cut(s)	155, 882
BseBI	CCWGG	5 cut(s)	239, 402, 1161, 1464, 1494
BseDI	CCNNGG	4 cut(s)	1409, 1432, 1564, 1747
BseGI	GGATG	3 cut(s)	1270, 1303, 1564
BseJI	GATNNNNATC	1 cut(s)	1179
BseLI	CCNNNNNNNGG	8 cut(s)	244, 255, 256, 697, 777, 1166, 1217, 1499
BseMII	CTCAG	4 cut(s)	233, 368, 719, 1050
BseNI	ACTGG	9 cut(s)	154, 426, 869, 899, 985, 1021, 1073, 1809, 1961
BseRI	GAGGAG	2 cut(s)	2016, 2019
BseSI	GKGCMC	2 cut(s)	353, 1409
BseX3I	CGGCCG	2 cut(s)	1246, 1429
Bsh1236I	CGCG	1 cut(s)	1839
Bsh1285I	CGRYCG	4 cut(s)	1249, 1366, 1399, 1432
BsiEI	CGRYCG	4 cut(s)	1249, 1366, 1399, 1432
BsiHKAI	GWGCWC	1 cut(s)	343
BsiSI	CCGG	6 cut(s)	156, 883, 1242, 1687, 1711, 1735
BslFI	GGGAC	7 cut(s)	674, 854, 1427, 1584, 1596, 1729, 1897
BslI	CCNNNNNNNGG	8 cut(s)	244, 255, 256, 697, 777, 1166, 1217, 1499
BsmAI	GTCTC	4 cut(s)	852, 855, 1913, 2001
BsmFI	GGGAC	7 cut(s)	674, 854, 1427, 1584, 1596, 1729, 1897
BsmI	GAATGC	2 cut(s)	185, 489
Bso31I	GGTCTC	1 cut(s)	2001
Bsp119I	TTCGAA	1 cut(s)	1657
Bsp120I	GGGCCC	2 cut(s)	349, 1405
Bsp1286I	GDGCHC	4 cut(s)	343, 353, 1409, 1808
Bsp13I	TCCGGA	2 cut(s)	155, 882
Bsp1407I	TGTACA	2 cut(s)	558, 619
BspCNI	CTCAG	4 cut(s)	232, 367, 720, 1051
BspEI	TCCGGA	2 cut(s)	155, 882
BspFNI	CGCG	1 cut(s)	1839
BspPI	GGATC	5 cut(s)	534, 718, 726, 1856, 1869
BspQI	GCTCTTC	1 cut(s)	120
BspT104I	TTCGAA	1 cut(s)	1657
BspTNI	GGTCTC	1 cut(s)	2001
BsrGI	TGTACA	2 cut(s)	558, 619
BsrI	ACTGG	9 cut(s)	154, 426, 869, 899, 985, 1021, 1073, 1809, 1961
BssECI	CCNNGG	4 cut(s)	1409, 1432, 1564, 1747
BssSI	CACGAG	1 cut(s)	927
BssT1I	CCWWGG	1 cut(s)	1409
Bst2BI	CACGAG	1 cut(s)	927
Bst2UI	CCWGG	5 cut(s)	239, 402, 1161, 1464, 1494
Bst4CI	ACNGT	6 cut(s)	235, 675, 1193, 1418, 1553, 1796
Bst6I	CTCTTC	1 cut(s)	120
BstAUI	TGTACA	2 cut(s)	558, 619
BstBAI	YACGTR	3 cut(s)	324, 800, 1151
BstBI	TTCGAA	1 cut(s)	1657
BstC8I	GCNNGC	9 cut(s)	67, 183, 264, 303, 651, 1027, 1228, 1343, 1376
BstDEI	CTNAG	6 cut(s)	197, 219, 354, 728, 750, 1059
BstDSI	CCRYGG	3 cut(s)	1432, 1564, 1747
BstF5I	GGATG	3 cut(s)	1270, 1303, 1564
BstFNI	CGCG	1 cut(s)	1839
BstHHI	GCGC	1 cut(s)	1031
BstMAI	GTCTC	4 cut(s)	852, 855, 1913, 2001
BstMCI	CGRYCG	4 cut(s)	1249, 1366, 1399, 1432
BstNI	CCWGG	5 cut(s)	239, 402, 1161, 1464, 1494
BstNSI	RCATGY	2 cut(s)	1345, 1378
BstPAI	GACNNNNGTC	1 cut(s)	1925
BstSCI	CCNGG	5 cut(s)	237, 400, 1159, 1462, 1492
BstSFI	CTRYAG	2 cut(s)	1633, 1765
BstSLI	GKGCMC	2 cut(s)	353, 1409
BstUI	CGCG	1 cut(s)	1839
BstV2I	GAAGAC	1 cut(s)	996
BstX2I	RGATCY	3 cut(s)	723, 731, 1861
BstYI	RGATCY	3 cut(s)	723, 731, 1861
BstZI	CGGCCG	2 cut(s)	1246, 1429
BsuI	GTATCC	1 cut(s)	1981
BtgI	CCRYGG	3 cut(s)	1432, 1564, 1747
BtrI	CACGTC	1 cut(s)	1574
BtsCI	GGATG	3 cut(s)	1270, 1303, 1564
BtsI	GCAGTG	1 cut(s)	1534
BtsIMutI	CAGTG	4 cut(s)	912, 1423, 1534, 1968
Cac8I	GCNNGC	9 cut(s)	67, 183, 264, 303, 651, 1027, 1228, 1343, 1376
CaiI	CAGNNNCTG	1 cut(s)	727
CfoI	GCGC	1 cut(s)	1031
CseI	GACGC	1 cut(s)	819
Csp6I	GTAC	6 cut(s)	464, 559, 620, 676, 1546, 1892
CspCI	CAANNNNNGTGG	2 cut(s)	901, 936
CviAII	CATG	7 cut(s)	59, 102, 493, 570, 1342, 1375, 1662
CviQI	GTAC	6 cut(s)	464, 559, 620, 676, 1546, 1892
DdeI	CTNAG	6 cut(s)	197, 219, 354, 728, 750, 1059
DraI	TTTAAA	1 cut(s)	873
EaeI	YGGCCR	6 cut(s)	104, 1228, 1246, 1429, 1561, 1750
EagI	CGGCCG	2 cut(s)	1246, 1429
Eam1104I	CTCTTC	1 cut(s)	120
EarI	CTCTTC	1 cut(s)	120
EciI	GGCGGA	1 cut(s)	2013
Ecl136II	GAGCTC	1 cut(s)	341
EclXI	CGGCCG	2 cut(s)	1246, 1429
Eco130I	CCWWGG	1 cut(s)	1409
Eco24I	GRGCYC	4 cut(s)	343, 353, 1409, 1808
Eco31I	GGTCTC	1 cut(s)	2001
Eco47I	GGWCC	2 cut(s)	1490, 1743
Eco52I	CGGCCG	2 cut(s)	1246, 1429
Eco53kI	GAGCTC	1 cut(s)	341
Eco57I	CTGAAG	1 cut(s)	365
Eco72I	CACGTG	1 cut(s)	324
EcoICRI	GAGCTC	1 cut(s)	341
EcoO109I	RGGNCCY	4 cut(s)	349, 350, 1405, 1406
EcoRII	CCWGG	5 cut(s)	237, 400, 1159, 1462, 1492
EcoT14I	CCWWGG	1 cut(s)	1409
EcoT38I	GRGCYC	4 cut(s)	343, 353, 1409, 1808
ErhI	CCWWGG	1 cut(s)	1409
FaeI	CATG	7 cut(s)	62, 105, 496, 573, 1345, 1378, 1665
FaqI	GGGAC	7 cut(s)	674, 854, 1427, 1584, 1596, 1729, 1897
FatI	CATG	7 cut(s)	58, 101, 492, 569, 1341, 1374, 1661
FauI	CCCGC	1 cut(s)	546
FbaI	TGATCA	1 cut(s)	787
FokI	GGATG	3 cut(s)	1277, 1310, 1571
FriOI	GRGCYC	4 cut(s)	343, 353, 1409, 1808
FspBI	CTAG	3 cut(s)	309, 1410, 1982
GlaI	GCGC	1 cut(s)	1030
GsuI	CTGGAG	2 cut(s)	1110, 1121
HapII	CCGG	6 cut(s)	156, 883, 1242, 1687, 1711, 1735
HgaI	GACGC	1 cut(s)	819
HhaI	GCGC	1 cut(s)	1031
Hin1II	CATG	7 cut(s)	62, 105, 496, 573, 1345, 1378, 1665
Hin6I	GCGC	1 cut(s)	1029
HinP1I	GCGC	1 cut(s)	1029
HinfI	GANTC	8 cut(s)	1180, 1199, 1238, 1690, 1720, 1726, 1874, 1954
HpaII	CCGG	6 cut(s)	156, 883, 1242, 1687, 1711, 1735
HphI	GGTGA	2 cut(s)	866, 992
Hpy166II	GTNNAC	4 cut(s)	561, 964, 1000, 1577
Hpy188I	TCNGA	6 cut(s)	222, 345, 792, 1060, 1725, 1803
Hpy188III	TCNNGA	8 cut(s)	156, 727, 883, 1100, 1127, 1610, 1871, 1940
Hpy8I	GTNNAC	4 cut(s)	561, 964, 1000, 1577
HpyAV	CCTTC	3 cut(s)	340, 1852, 2022
HpyCH4III	ACNGT	6 cut(s)	235, 675, 1193, 1418, 1553, 1796
HpyCH4IV	ACGT	7 cut(s)	323, 678, 799, 918, 1150, 1573, 1910
HpyCH4V	TGCA	9 cut(s)	137, 143, 185, 266, 321, 656, 797, 830, 1079
HpyF3I	CTNAG	6 cut(s)	197, 219, 354, 728, 750, 1059
HpySE526I	ACGT	7 cut(s)	323, 678, 799, 918, 1150, 1573, 1910
Hsp92II	CATG	7 cut(s)	62, 105, 496, 573, 1345, 1378, 1665
HspAI	GCGC	1 cut(s)	1029
Kpn2I	TCCGGA	2 cut(s)	155, 882
Ksp22I	TGATCA	1 cut(s)	787
LguI	GCTCTTC	1 cut(s)	120
LmnI	GCTCC	9 cut(s)	10, 259, 338, 377, 718, 1106, 1617, 1737, 1803
LweI	GCATC	2 cut(s)	83, 168
MaeI	CTAG	3 cut(s)	309, 1410, 1982
MaeII	ACGT	7 cut(s)	323, 678, 799, 918, 1150, 1573, 1910
MboII	GAAGA	6 cut(s)	107, 185, 442, 596, 1001, 1023
MfeI	CAATTG	2 cut(s)	108, 138
MflI	RGATCY	3 cut(s)	723, 731, 1861
MhlI	GDGCHC	4 cut(s)	343, 353, 1409, 1808
MlsI	TGGCCA	1 cut(s)	106
MluI	ACGCGT	1 cut(s)	1837
MluNI	TGGCCA	1 cut(s)	106
MlyI	GAGTC	3 cut(s)	1699, 1729, 1735
MmeI	TCCRAC	2 cut(s)	1703, 1781
Mox20I	TGGCCA	1 cut(s)	106
MroI	TCCGGA	2 cut(s)	155, 882
MscI	TGGCCA	1 cut(s)	106
MseI	TTAA	8 cut(s)	411, 612, 642, 872, 1092, 1215, 1671, 2032
MslI	CAYNNNNRTG	2 cut(s)	100, 389
Msp20I	TGGCCA	1 cut(s)	106
MspA1I	CMGCKG	1 cut(s)	1082
MspI	CCGG	6 cut(s)	156, 883, 1242, 1687, 1711, 1735
MspR9I	CCNGG	5 cut(s)	239, 402, 1161, 1464, 1494
MunI	CAATTG	2 cut(s)	108, 138
Mva1269I	GAATGC	2 cut(s)	185, 489
MvaI	CCWGG	5 cut(s)	239, 402, 1161, 1464, 1494
MvnI	CGCG	1 cut(s)	1839
NlaIII	CATG	7 cut(s)	62, 105, 496, 573, 1345, 1378, 1665
NspI	RCATGY	2 cut(s)	1345, 1378
NspV	TTCGAA	1 cut(s)	1657
PaeI	GCATGC	2 cut(s)	1345, 1378
PciSI	GCTCTTC	1 cut(s)	120
PctI	GAATGC	2 cut(s)	185, 489
PfeI	GAWTC	5 cut(s)	1180, 1199, 1238, 1874, 1954
PleI	GAGTC	3 cut(s)	1698, 1728, 1734
PmaCI	CACGTG	1 cut(s)	324
PmlI	CACGTG	1 cut(s)	324
PpsI	GAGTC	3 cut(s)	1698, 1728, 1734
Ppu21I	YACGTR	3 cut(s)	324, 800, 1151
PshAI	GACNNNNGTC	1 cut(s)	1925
Psp124BI	GAGCTC	1 cut(s)	343
Psp6I	CCWGG	5 cut(s)	237, 400, 1159, 1462, 1492
PspCI	CACGTG	1 cut(s)	324
PspGI	CCWGG	5 cut(s)	237, 400, 1159, 1462, 1492
PspOMI	GGGCCC	2 cut(s)	349, 1405
PstNI	CAGNNNCTG	1 cut(s)	727
PsuI	RGATCY	3 cut(s)	723, 731, 1861
PvuII	CAGCTG	1 cut(s)	1082
RsaI	GTAC	6 cut(s)	465, 560, 621, 677, 1547, 1893
RsaNI	GTAC	6 cut(s)	464, 559, 620, 676, 1546, 1892
RseI	CAYNNNNRTG	2 cut(s)	100, 389
SacI	GAGCTC	1 cut(s)	343
SapI	GCTCTTC	1 cut(s)	120
SaqAI	TTAA	8 cut(s)	411, 612, 642, 872, 1092, 1215, 1671, 2032
SchI	GAGTC	3 cut(s)	1699, 1729, 1735
ScrFI	CCNGG	5 cut(s)	239, 402, 1161, 1464, 1494
SduI	GDGCHC	4 cut(s)	343, 353, 1409, 1808
SfaNI	GCATC	2 cut(s)	83, 168
SfcI	CTRYAG	2 cut(s)	1633, 1765
SfuI	TTCGAA	1 cut(s)	1657
SinI	GGWCC	2 cut(s)	1490, 1743
SmiMI	CAYNNNNRTG	2 cut(s)	100, 389
SmlI	CTYRAG	2 cut(s)	42, 849
SmoI	CTYRAG	2 cut(s)	42, 849
SphI	GCATGC	2 cut(s)	1345, 1378
SspI	AATATT	1 cut(s)	631
SspMI	CTAG	3 cut(s)	309, 1410, 1982
SstI	GAGCTC	1 cut(s)	343
StyD4I	CCNGG	5 cut(s)	237, 400, 1159, 1462, 1492
StyI	CCWWGG	1 cut(s)	1409
TaaI	ACNGT	6 cut(s)	235, 675, 1193, 1418, 1553, 1796
TaiI	ACGT	7 cut(s)	326, 681, 802, 921, 1153, 1576, 1913
TaqI	TCGA	3 cut(s)	481, 1657, 1693
TatI	WGTACW	3 cut(s)	463, 558, 619
TauI	GCSGC	6 cut(s)	1248, 1341, 1431, 1701, 1761, 1834
TfiI	GAWTC	5 cut(s)	1180, 1199, 1238, 1874, 1954
Tru1I	TTAA	8 cut(s)	411, 612, 642, 872, 1092, 1215, 1671, 2032
Tru9I	TTAA	8 cut(s)	411, 612, 642, 872, 1092, 1215, 1671, 2032
TscAI	CASTG	4 cut(s)	919, 1423, 1534, 1968
TspDTI	ATGAA	7 cut(s)	47, 178, 443, 486, 1202, 1830, 1871
TspGWI	ACGGA	1 cut(s)	503
TspRI	CASTG	4 cut(s)	919, 1423, 1534, 1968
VpaK11BI	GGWCC	2 cut(s)	1490, 1743
XapI	RAATTY	2 cut(s)	293, 1847
XceI	RCATGY	2 cut(s)	1345, 1378
XmaJI	CCTAGG	1 cut(s)	1409
XspI	CTAG	3 cut(s)	309, 1410, 1982

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.