Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0006634

Physical Location & Seq

Genomic Coordinates Forward (+)

3186038 .. 3188540

Gene Structure 2503 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT3G10300.4

Sequence Viewer

CDS Protein Promoter (2k)

Length: 699 bp

ATGTCTGGTTATCCTCCATCAAGCCAAGGTTACGGTTACGGCGGTAATCCACCACCTCCTCAGCCACCGTACGGATCAACCGGCAATAACCCTCCTCCGTACGGATCATCCGGCAGCAATCCTCCTCCTCCGTACGGATCATCAGCCTCCTCACCGTACGCAGTTCCCTACGGAGCTCAGCCCGCTCCTTACGGTGCACCACCGTCAGCACCGTACGCGTCTCTTCCAGGAGACCATAATAAGCCGCACAAAGAGAAACCTCACGGCGCCTCCTACGGATCTCCATCTCCCGGTGGCTACGGTGCTCATCCATCGTCTGGACCTTCCGACTACGGTGGTTACGGAGGAGCGCCGCAGCAGTCTGGACATGGAGGAGGTTACGGAGGAGCGCCGCAGCAGTCTGGACATGGAGGAGGTTACGGAGCTCCTCCTCCGCAAGCTTCTTATGGAAGTCCGTTTGCGTCTCTGGTTCCGTCGGCGTTTCCTCCCGGAACAGATCCGAACATTGTGGCTTGTTTCCAAGCTGCGGATCGGGACAATAGTGGATTCATCGATGATAAGGAGCTTCAAGGAGCTCTATCTTCGTATAATCAGAGCTTCAGCATAAGAACTGTTCATCTCCTTATGTATCTATTCACCAACAGCAATGTCAGGAAGATTGGTCAGTTTCTTCTTTCTTTCAAACTGATCTCAAATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004175 GO:0004197 GO:0004198 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0016787 GO:0019538 GO:0043170 GO:0044238 GO:0044424 GO:0044464 GO:0070011 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04626 map04626

Pfam Domains

Protein Families

EF-hand_1 EF-hand_5 EF-hand_6

Protein Analysis

232

Amino Acids

23.55

Weight (kDa)

7.05

Isoelectric Point (pI)

61.73

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EF-hand_6	PF13405	235 - 263	3.5e-08	EF-hand domain
EF-hand_7	PF13499	235 - 296	6.9e-09	EF-hand domain pair
EF-hand_1	PF00036	235 - 262	9.7e-07	EF hand domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0006634)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G10300 AT3G10300 AT3G10300 AT3G10300 AT3G10300 AT3G10300 AT5G04170
fragaria_vesca	FvH4_6g24530
malus_domestica	MD12G1082900.v1.1 MD14G1061500.v1.1 MD14G1077400.v1.1 MD14G1077700.v1.1
prunus_persica	Prupe.7G053100_v2.0.a1
pyrus_communis	pycom12g06980 pycom14g06630 pycom14g06640
rosa_chinensis	RchiOBHm_Chr3g0483641
rosa_laevigata	RLG00000023262
rosa_multiflora	Rmu_sc0000555.1_g000008
rosa_roxburghii	Rroxscaffold_6G00398160
rosa_rugosa	Rorug03G0207700
rosa_samantha	Rh3AG256100 Rh3BG292800 Rh3CG290500 Rh3DG286400
rosa_wichuraiana	Rw3G023130

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	266
AccB7I	CCANNNNNTGG	1 cut(s)	317
AccBSI	CCGCTC	1 cut(s)	185
AccII	CGCG	1 cut(s)	218
AciI	CCGC	7 cut(s)	42, 183, 245, 353, 392, 434, 527
AclWI	GGATC	6 cut(s)	82, 112, 145, 286, 491, 537
AcuI	CTGAAG	1 cut(s)	583
AcyI	GRCGYC	1 cut(s)	267
AfaI	GTAC	5 cut(s)	71, 101, 134, 158, 215
AfiI	CCNNNNNNNGG	6 cut(s)	71, 101, 134, 290, 317, 526
AflIII	ACRYGT	1 cut(s)	216
AgsI	TTSAA	2 cut(s)	569, 682
AjnI	CCWGG	1 cut(s)	226
AluBI	AGCT	7 cut(s)	176, 425, 440, 524, 565, 575, 597
AluI	AGCT	7 cut(s)	176, 425, 440, 524, 565, 575, 597
Alw21I	GWGCWC	5 cut(s)	178, 199, 307, 427, 577
Alw26I	GTCTC	3 cut(s)	225, 225, 468
Alw44I	GTGCAC	1 cut(s)	195
AlwI	GGATC	6 cut(s)	82, 112, 145, 286, 491, 537
ApaLI	GTGCAC	1 cut(s)	195
ApeKI	GCWGC	4 cut(s)	114, 355, 394, 524
AspLEI	GCGC	3 cut(s)	269, 352, 391
AspS9I	GGNCC	1 cut(s)	320
AsuC2I	CCSGG	2 cut(s)	291, 489
AsuHPI	GGTGA	2 cut(s)	144, 628
AvaII	GGWCC	1 cut(s)	320
BaeGI	GKGCMC	1 cut(s)	199
BanI	GGYRCC	1 cut(s)	266
BanII	GRGCYC	3 cut(s)	178, 427, 577
Bbv12I	GWGCWC	5 cut(s)	178, 199, 307, 427, 577
BbvCI	CCTCAGC	1 cut(s)	60
BbvI	GCAGC	4 cut(s)	126, 367, 406, 511
BccI	CCATC	3 cut(s)	25, 292, 319
BceAI	ACGGC	2 cut(s)	55, 280
BciT130I	CCWGG	1 cut(s)	228
BcnI	CCSGG	2 cut(s)	291, 489
BcoDI	GTCTC	3 cut(s)	225, 225, 468
BfoI	RGCGCY	3 cut(s)	270, 353, 392
BisI	GCNGC	7 cut(s)	115, 245, 353, 356, 392, 395, 525
BlpI	GCTNAGC	1 cut(s)	177
BlsI	GCNGC	7 cut(s)	116, 246, 354, 357, 393, 396, 526
Bme1390I	CCNGG	3 cut(s)	228, 291, 489
Bme18I	GGWCC	1 cut(s)	320
BmgT120I	GGNCC	1 cut(s)	320
BmiI	GGNNCC	2 cut(s)	268, 471
BmrFI	CCNGG	3 cut(s)	228, 291, 489
Bpu10I	CCTNAGC	1 cut(s)	60
Bpu1102I	GCTNAGC	1 cut(s)	177
BpuMI	CCSGG	2 cut(s)	291, 489
Bsa29I	ATCGAT	1 cut(s)	552
BsaBI	GATNNNNATC	1 cut(s)	283
BsaHI	GRCGYC	1 cut(s)	267
BsaI	GGTCTC	1 cut(s)	225
BsaJI	CCNNGG	1 cut(s)	25
BsaXI	ACNNNNNCTCC	2 cut(s)	254, 284
Bsc4I	CCNNNNNNNGG	6 cut(s)	71, 101, 134, 290, 317, 526
Bse118I	RCCGGY	1 cut(s)	80
Bse3DI	GCAATG	1 cut(s)	652
Bse8I	GATNNNNATC	1 cut(s)	283
BseBI	CCWGG	1 cut(s)	228
BseCI	ATCGAT	1 cut(s)	552
BseDI	CCNNGG	1 cut(s)	25
BseGI	GGATG	2 cut(s)	107, 307
BseJI	GATNNNNATC	1 cut(s)	283
BseLI	CCNNNNNNNGG	6 cut(s)	71, 101, 134, 290, 317, 526
BseMI	GCAATG	1 cut(s)	652
BseMII	CTCAG	2 cut(s)	74, 191
BseSI	GKGCMC	1 cut(s)	199
BseXI	GCAGC	4 cut(s)	126, 367, 406, 511
Bsh1236I	CGCG	1 cut(s)	218
BshNI	GGYRCC	1 cut(s)	266
BshVI	ATCGAT	1 cut(s)	552
BsiHKAI	GWGCWC	5 cut(s)	178, 199, 307, 427, 577
BsiSI	CCGG	4 cut(s)	81, 111, 291, 489
BsiWI	CGTACG	5 cut(s)	69, 99, 132, 156, 213
BslFI	GGGAC	1 cut(s)	548
BslI	CCNNNNNNNGG	6 cut(s)	71, 101, 134, 290, 317, 526
BsmAI	GTCTC	3 cut(s)	225, 225, 468
BsmBI	CGTCTC	2 cut(s)	225, 468
BsmFI	GGGAC	1 cut(s)	548
Bso31I	GGTCTC	1 cut(s)	225
Bsp1286I	GDGCHC	5 cut(s)	178, 199, 307, 427, 577
Bsp143I	GATC	7 cut(s)	74, 104, 137, 278, 496, 529, 687
Bsp1720I	GCTNAGC	1 cut(s)	177
BspACI	CCGC	7 cut(s)	42, 183, 245, 353, 392, 434, 527
BspCNI	CTCAG	2 cut(s)	73, 190
BspDI	ATCGAT	1 cut(s)	552
BspFNI	CGCG	1 cut(s)	218
BspLI	GGNNCC	2 cut(s)	268, 471
BspPI	GGATC	6 cut(s)	82, 112, 145, 286, 491, 537
BspT107I	GGYRCC	1 cut(s)	266
BspTNI	GGTCTC	1 cut(s)	225
BsrBI	CCGCTC	1 cut(s)	185
BsrDI	GCAATG	1 cut(s)	652
BsrFI	RCCGGY	1 cut(s)	80
BssAI	RCCGGY	1 cut(s)	80
BssECI	CCNNGG	1 cut(s)	25
BssMI	GATC	7 cut(s)	74, 104, 137, 278, 496, 529, 687
BssNI	GRCGYC	1 cut(s)	267
BssT1I	CCWWGG	1 cut(s)	25
Bst2UI	CCWGG	1 cut(s)	228
Bst4CI	ACNGT	9 cut(s)	35, 69, 156, 194, 204, 213, 302, 335, 613
Bst6I	CTCTTC	1 cut(s)	228
BstACI	GRCGYC	1 cut(s)	267
BstC8I	GCNNGC	2 cut(s)	183, 438
BstDEI	CTNAG	2 cut(s)	60, 177
BstF5I	GGATG	2 cut(s)	107, 307
BstFNI	CGCG	1 cut(s)	218
BstH2I	RGCGCY	3 cut(s)	270, 353, 392
BstHHI	GCGC	3 cut(s)	269, 352, 391
BstKTI	GATC	7 cut(s)	77, 107, 140, 281, 499, 532, 690
BstMAI	GTCTC	3 cut(s)	225, 225, 468
BstMBI	GATC	7 cut(s)	74, 104, 137, 278, 496, 529, 687
BstMWI	GCNNNNNNNGC	2 cut(s)	182, 215
BstNI	CCWGG	1 cut(s)	228
BstSCI	CCNGG	3 cut(s)	226, 289, 487
BstSLI	GKGCMC	1 cut(s)	199
BstUI	CGCG	1 cut(s)	218
BstV1I	GCAGC	4 cut(s)	126, 367, 406, 511
BstX2I	RGATCY	2 cut(s)	278, 496
BstYI	RGATCY	2 cut(s)	278, 496
Bsu15I	ATCGAT	1 cut(s)	552
BsuTUI	ATCGAT	1 cut(s)	552
BtsCI	GGATG	2 cut(s)	107, 307
Cac8I	GCNNGC	2 cut(s)	183, 438
CfoI	GCGC	3 cut(s)	269, 352, 391
Cfr10I	RCCGGY	1 cut(s)	80
Cfr13I	GGNCC	1 cut(s)	320
ClaI	ATCGAT	1 cut(s)	552
CseI	GACGC	2 cut(s)	207, 450
Csp6I	GTAC	5 cut(s)	70, 100, 133, 157, 214
CviAII	CATG	2 cut(s)	368, 407
CviQI	GTAC	5 cut(s)	70, 100, 133, 157, 214
DdeI	CTNAG	2 cut(s)	60, 177
DinI	GGCGCC	1 cut(s)	268
DpnI	GATC	7 cut(s)	76, 106, 139, 280, 498, 531, 689
DpnII	GATC	7 cut(s)	74, 104, 137, 278, 496, 529, 687
Eam1104I	CTCTTC	1 cut(s)	228
EarI	CTCTTC	1 cut(s)	228
Ecl136II	GAGCTC	3 cut(s)	176, 425, 575
Eco130I	CCWWGG	1 cut(s)	25
Eco24I	GRGCYC	3 cut(s)	178, 427, 577
Eco31I	GGTCTC	1 cut(s)	225
Eco47I	GGWCC	1 cut(s)	320
Eco53kI	GAGCTC	3 cut(s)	176, 425, 575
Eco57I	CTGAAG	1 cut(s)	583
EcoICRI	GAGCTC	3 cut(s)	176, 425, 575
EcoRII	CCWGG	1 cut(s)	226
EcoT14I	CCWWGG	1 cut(s)	25
EcoT38I	GRGCYC	3 cut(s)	178, 427, 577
EgeI	GGCGCC	1 cut(s)	268
EheI	GGCGCC	1 cut(s)	268
ErhI	CCWWGG	1 cut(s)	25
Esp3I	CGTCTC	2 cut(s)	225, 468
FaeI	CATG	2 cut(s)	371, 410
FaiI	YATR	7 cut(s)	237, 369, 408, 447, 588, 605, 626
FaqI	GGGAC	1 cut(s)	548
FatI	CATG	2 cut(s)	367, 406
FauI	CCCGC	1 cut(s)	190
Fnu4HI	GCNGC	7 cut(s)	115, 245, 353, 356, 392, 395, 525
FokI	GGATG	2 cut(s)	94, 294
FriOI	GRGCYC	3 cut(s)	178, 427, 577
Fsp4HI	GCNGC	7 cut(s)	115, 245, 353, 356, 392, 395, 525
GlaI	GCGC	3 cut(s)	268, 351, 390
GluI	GCNGC	7 cut(s)	115, 245, 353, 356, 392, 395, 525
HaeII	RGCGCY	3 cut(s)	270, 353, 392
HapII	CCGG	4 cut(s)	81, 111, 291, 489
HgaI	GACGC	2 cut(s)	207, 450
HhaI	GCGC	3 cut(s)	269, 352, 391
Hin1I	GRCGYC	1 cut(s)	267
Hin1II	CATG	2 cut(s)	371, 410
Hin6I	GCGC	3 cut(s)	267, 350, 389
HinP1I	GCGC	3 cut(s)	267, 350, 389
HindIII	AAGCTT	1 cut(s)	438
HinfI	GANTC	1 cut(s)	546
HpaII	CCGG	4 cut(s)	81, 111, 291, 489
HphI	GGTGA	2 cut(s)	144, 628
Hpy166II	GTNNAC	1 cut(s)	197
Hpy188I	TCNGA	3 cut(s)	328, 501, 594
Hpy188III	TCNNGA	5 cut(s)	318, 363, 402, 533, 652
Hpy8I	GTNNAC	1 cut(s)	197
Hpy99I	CGWCG	1 cut(s)	478
HpyAV	CCTTC	1 cut(s)	333
HpyCH4III	ACNGT	9 cut(s)	35, 69, 156, 194, 204, 213, 302, 335, 613
HpyCH4V	TGCA	1 cut(s)	197
HpyF10VI	GCNNNNNNNGC	2 cut(s)	182, 215
HpyF3I	CTNAG	2 cut(s)	60, 177
Hsp92I	GRCGYC	1 cut(s)	267
Hsp92II	CATG	2 cut(s)	371, 410
HspAI	GCGC	3 cut(s)	267, 350, 389
KasI	GGCGCC	1 cut(s)	266
Kzo9I	GATC	7 cut(s)	74, 104, 137, 278, 496, 529, 687
LmnI	GCTCC	8 cut(s)	173, 190, 347, 386, 422, 430, 562, 572
Lsp1109I	GCAGC	4 cut(s)	126, 367, 406, 511
MaeIII	GTNAC	5 cut(s)	29, 35, 338, 377, 416
MalI	GATC	7 cut(s)	76, 106, 139, 280, 498, 531, 689
MbiI	CCGCTC	1 cut(s)	185
MboI	GATC	7 cut(s)	74, 104, 137, 278, 496, 529, 687
MboII	GAAGA	4 cut(s)	215, 573, 662, 667
MflI	RGATCY	2 cut(s)	278, 496
MhlI	GDGCHC	5 cut(s)	178, 199, 307, 427, 577
MluCI	AATT	1 cut(s)	694
MluI	ACGCGT	1 cut(s)	216
Mly113I	GGCGCC	1 cut(s)	267
MmeI	TCCRAC	1 cut(s)	351
MspI	CCGG	4 cut(s)	81, 111, 291, 489
MspR9I	CCNGG	3 cut(s)	228, 291, 489
MvaI	CCWGG	1 cut(s)	228
MvnI	CGCG	1 cut(s)	218
MwoI	GCNNNNNNNGC	2 cut(s)	182, 215
NarI	GGCGCC	1 cut(s)	267
NciI	CCSGG	2 cut(s)	291, 489
NdeII	GATC	7 cut(s)	74, 104, 137, 278, 496, 529, 687
NlaIII	CATG	2 cut(s)	371, 410
NlaIV	GGNNCC	2 cut(s)	268, 471
PfeI	GAWTC	1 cut(s)	546
Pfl23II	CGTACG	5 cut(s)	69, 99, 132, 156, 213
PflMI	CCANNNNNTGG	1 cut(s)	317
PfoI	TCCNGGA	2 cut(s)	226, 487
PkrI	GCNGC	7 cut(s)	116, 246, 354, 357, 393, 396, 526
PluTI	GGCGCC	1 cut(s)	270
Psp124BI	GAGCTC	3 cut(s)	178, 427, 577
Psp6I	CCWGG	1 cut(s)	226
PspGI	CCWGG	1 cut(s)	226
PspLI	CGTACG	5 cut(s)	69, 99, 132, 156, 213
PspN4I	GGNNCC	2 cut(s)	268, 471
PspPI	GGNCC	1 cut(s)	320
PsuI	RGATCY	2 cut(s)	278, 496
RsaI	GTAC	5 cut(s)	71, 101, 134, 158, 215
RsaNI	GTAC	5 cut(s)	70, 100, 133, 157, 214
SacI	GAGCTC	3 cut(s)	178, 427, 577
SatI	GCNGC	7 cut(s)	115, 245, 353, 356, 392, 395, 525
Sau3AI	GATC	7 cut(s)	74, 104, 137, 278, 496, 529, 687
Sau96I	GGNCC	1 cut(s)	320
ScrFI	CCNGG	3 cut(s)	228, 291, 489
SduI	GDGCHC	5 cut(s)	178, 199, 307, 427, 577
SfoI	GGCGCC	1 cut(s)	268
SinI	GGWCC	1 cut(s)	320
Sse9I	AATT	1 cut(s)	694
SsiI	CCGC	7 cut(s)	42, 183, 245, 353, 392, 434, 527
SspDI	GGCGCC	1 cut(s)	266
SstI	GAGCTC	3 cut(s)	178, 427, 577
StyD4I	CCNGG	3 cut(s)	226, 289, 487
StyI	CCWWGG	1 cut(s)	25
TaaI	ACNGT	9 cut(s)	35, 69, 156, 194, 204, 213, 302, 335, 613
TaqI	TCGA	1 cut(s)	552
TasI	AATT	1 cut(s)	694
TauI	GCSGC	3 cut(s)	247, 355, 394
TfiI	GAWTC	1 cut(s)	546
TseI	GCWGC	4 cut(s)	114, 355, 394, 524
TspDTI	ATGAA	2 cut(s)	538, 605
Van91I	CCANNNNNTGG	1 cut(s)	317
VneI	GTGCAC	1 cut(s)	195
VpaK11BI	GGWCC	1 cut(s)	320

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.