Prupe.7G053100_v2.0.a1

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp07
OG0006634
Physical Location & Seq
Reverse (-)
9135133 .. 9137539
2407 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.7G053100.1

Sequence Viewer

Length: 840 bp
ATGTCTGGATACCCACACCCGCCCCCCGGCTACGGCTACGGCAGCACGCCTCCACCAGGCAAACCCTACGCCGCGCCGCAGCCAGGCCAGCCAGGCCAACCCGGCCAGCCCCACCAACACCAAGCGCAACCGTACGGAGCGCCGGCTGCACCTTATGGCGCGCCGTCTGCACCCTACGGCGCACCATCGGCACCGTACGCCGCGCCGTACGCGCCAGAGAAGCCTCACCAGAGCCACGGAAGCAGCGGGGGTGGATACCCGCCGTCGGCTCCGGGCTACGGAAGCCCGTTCGCGGCTCTGGTGCCATCGTCGTTCCCACCCGGTACGGACCCGAATGTGGTGGCGTGCTTTCAGATGGCGGATCAGGACGGCAGTGGCTTCATCGACGACAAGGAGTTGCAGAGAGCTCTGTCGTCATACAATCAGAGCTTCAGCTTGAGGACTGTTCATCTTCTCATGTACCTCTTCACCCAAACCAACACCAGGAAGATCGGGCCTAAGGAATTCACTGCAGTGTTCTACAGTCTTCAGAGTTGGAGGGGAATATTTGAGAGGTTCGACAGGGATAGAAGTGGGCACATTGATGCAACTGAGCTGAGAGATGCACTGCTGAGTCTGGGTTTTTCCGTGTCACCTACGGTTTTGGATCTGCTGGTCTCTAAATTCGACAAGACCGGAGGCAAACACAGGGCCATTGAATATGACAATTTCATCGAGTGCTGTCTTACTGTTAAGGGTTTAACCGAAAAATTTAAGGAGAAGGACAAAGCGTACACCGGTACTGGAACTTTCAGCTATGAGGACTTCATGTTGACTGTGCTGCCTTTCCTCATTGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_6

Protein Analysis

280

Amino Acids

30.12

Weight (kDa)

6.44

Isoelectric Point (pI)

56.12

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 190, 301
AccII CGCG 5 cut(s) 74, 161, 203, 212, 293
AciI CCGC 8 cut(s) 20, 72, 77, 201, 246, 260, 293, 359
AclWI GGATC 2 cut(s) 369, 654
AcoI YGGCCR 1 cut(s) 103
AcsI RAATTY 3 cut(s) 503, 662, 749
AcuI CTGAAG 2 cut(s) 415, 512
AfaI GTAC 7 cut(s) 134, 197, 209, 325, 461, 773, 781
AfiI CCNNNNNNNGG 9 cut(s) 26, 32, 56, 83, 265, 278, 292, 337, 483
AgeI ACCGGT 1 cut(s) 776
AgsI TTSAA 1 cut(s) 698
AjnI CCWGG 4 cut(s) 55, 82, 91, 482
AleI CACNNNNGTG 1 cut(s) 512
AluBI AGCT 5 cut(s) 407, 429, 435, 595, 795
AluI AGCT 5 cut(s) 407, 429, 435, 595, 795
Alw21I GWGCWC 1 cut(s) 409
Alw26I GTCTC 1 cut(s) 661
AlwI GGATC 2 cut(s) 369, 654
AoxI GGCC 5 cut(s) 85, 94, 103, 494, 690
ApeKI GCWGC 5 cut(s) 42, 79, 146, 243, 820
ApoI RAATTY 3 cut(s) 503, 662, 749
AscI GGCGCGCC 1 cut(s) 159
AsiGI ACCGGT 1 cut(s) 776
AspLEI GCGC 8 cut(s) 76, 127, 142, 161, 163, 182, 205, 214
AspS9I GGNCC 3 cut(s) 328, 494, 690
AsuC2I CCSGG 4 cut(s) 27, 102, 273, 321
AsuHPI GGTGA 3 cut(s) 218, 460, 624
AvaII GGWCC 1 cut(s) 328
AxyI CCTNAGG 1 cut(s) 498
BaeGI GKGCMC 1 cut(s) 579
BanI GGYRCC 2 cut(s) 190, 301
BanII GRGCYC 1 cut(s) 409
BbsI GAAGAC 1 cut(s) 518
Bbv12I GWGCWC 1 cut(s) 409
BbvI GCAGC 5 cut(s) 54, 91, 133, 255, 807
BccI CCATC 3 cut(s) 193, 313, 349
BceAI ACGGC 7 cut(s) 49, 55, 148, 190, 193, 247, 385
BciT130I CCWGG 4 cut(s) 57, 84, 93, 484
BciVI GTATCC 1 cut(s) 248
BcnI CCSGG 4 cut(s) 27, 102, 273, 321
BcoDI GTCTC 1 cut(s) 661
BfmI CTRYAG 2 cut(s) 510, 520
BfoI RGCGCY 1 cut(s) 143
BfuI GTATCC 1 cut(s) 248
BglI GCCNNNNNGGC 2 cut(s) 93, 102
BisI GCNGC 9 cut(s) 43, 72, 77, 80, 147, 201, 244, 294, 821
BlsI GCNGC 9 cut(s) 44, 73, 78, 81, 148, 202, 245, 295, 822
Bme1390I CCNGG 8 cut(s) 27, 57, 84, 93, 102, 273, 321, 484
Bme18I GGWCC 1 cut(s) 328
BmgT120I GGNCC 3 cut(s) 328, 494, 690
BmiI GGNNCC 4 cut(s) 192, 270, 303, 330
BmrFI CCNGG 8 cut(s) 27, 57, 84, 93, 102, 273, 321, 484
BmsI GCATC 2 cut(s) 574, 592
BpiI GAAGAC 1 cut(s) 518
BpuEI CTTGAG 1 cut(s) 457
BpuMI CCSGG 4 cut(s) 27, 102, 273, 321
BsaI GGTCTC 1 cut(s) 661
BsaJI CCNNGG 2 cut(s) 25, 235
BsaWI WCCGGW 2 cut(s) 674, 776
Bsc4I CCNNNNNNNGG 9 cut(s) 26, 32, 56, 83, 265, 278, 292, 337, 483
Bse118I RCCGGY 2 cut(s) 142, 776
Bse1I ACTGG 1 cut(s) 787
Bse21I CCTNAGG 1 cut(s) 498
Bse3DI GCAATG 1 cut(s) 831
BseBI CCWGG 4 cut(s) 57, 84, 93, 484
BseDI CCNNGG 2 cut(s) 25, 235
BseLI CCNNNNNNNGG 9 cut(s) 26, 32, 56, 83, 265, 278, 292, 337, 483
BseMI GCAATG 1 cut(s) 831
BseMII CTCAG 3 cut(s) 582, 587, 602
BseNI ACTGG 1 cut(s) 787
BsePI GCGCGC 1 cut(s) 159
BseSI GKGCMC 1 cut(s) 579
BseXI GCAGC 5 cut(s) 54, 91, 133, 255, 807
BsgI GTGCAG 2 cut(s) 132, 153
Bsh1236I CGCG 5 cut(s) 74, 161, 203, 212, 293
BshFI GGCC 5 cut(s) 87, 96, 105, 496, 692
BshNI GGYRCC 2 cut(s) 190, 301
BshTI ACCGGT 1 cut(s) 776
BsiHKAI GWGCWC 1 cut(s) 409
BsiSI CCGG 7 cut(s) 27, 102, 143, 272, 321, 675, 777
BsiWI CGTACG 3 cut(s) 132, 195, 207
BslI CCNNNNNNNGG 9 cut(s) 26, 32, 56, 83, 265, 278, 292, 337, 483
BsmAI GTCTC 1 cut(s) 661
BsnI GGCC 5 cut(s) 87, 96, 105, 496, 692
Bso31I GGTCTC 1 cut(s) 661
Bsp1286I GDGCHC 2 cut(s) 409, 579
Bsp143I GATC 3 cut(s) 361, 489, 646
BspACI CCGC 8 cut(s) 20, 72, 77, 201, 246, 260, 293, 359
BspANI GGCC 5 cut(s) 87, 96, 105, 496, 692
BspCNI CTCAG 3 cut(s) 583, 588, 603
BspFNI CGCG 5 cut(s) 74, 161, 203, 212, 293
BspLI GGNNCC 4 cut(s) 192, 270, 303, 330
BspMAI CTGCAG 1 cut(s) 514
BspPI GGATC 2 cut(s) 369, 654
BspT107I GGYRCC 2 cut(s) 190, 301
BspTNI GGTCTC 1 cut(s) 661
BsrDI GCAATG 1 cut(s) 831
BsrFI RCCGGY 2 cut(s) 142, 776
BsrI ACTGG 1 cut(s) 787
BssAI RCCGGY 2 cut(s) 142, 776
BssECI CCNNGG 2 cut(s) 25, 235
BssHII GCGCGC 1 cut(s) 159
BssMI GATC 3 cut(s) 361, 489, 646
Bst2UI CCWGG 4 cut(s) 57, 84, 93, 484
Bst4CI ACNGT 7 cut(s) 132, 195, 445, 524, 640, 730, 817
Bst6I CTCTTC 1 cut(s) 470
BstC8I GCNNGC 6 cut(s) 47, 89, 107, 144, 161, 346
BstDEI CTNAG 4 cut(s) 498, 591, 596, 611
BstDSI CCRYGG 1 cut(s) 235
BstENI CCTNNNNNAGG 1 cut(s) 54
BstFNI CGCG 5 cut(s) 74, 161, 203, 212, 293
BstH2I RGCGCY 1 cut(s) 143
BstHHI GCGC 8 cut(s) 76, 127, 142, 161, 163, 182, 205, 214
BstKTI GATC 3 cut(s) 364, 492, 649
BstMAI GTCTC 1 cut(s) 661
BstMBI GATC 3 cut(s) 361, 489, 646
BstNI CCWGG 4 cut(s) 57, 84, 93, 484
BstSCI CCNGG 8 cut(s) 25, 55, 82, 91, 100, 271, 319, 482
BstSFI CTRYAG 2 cut(s) 510, 520
BstSLI GKGCMC 1 cut(s) 579
BstUI CGCG 5 cut(s) 74, 161, 203, 212, 293
BstV1I GCAGC 5 cut(s) 54, 91, 133, 255, 807
BstV2I GAAGAC 1 cut(s) 518
BstX2I RGATCY 1 cut(s) 646
BstYI RGATCY 1 cut(s) 646
Bsu36I CCTNAGG 1 cut(s) 498
BsuI GTATCC 1 cut(s) 248
BsuRI GGCC 5 cut(s) 87, 96, 105, 496, 692
BtgI CCRYGG 1 cut(s) 235
BtsI GCAGTG 4 cut(s) 379, 507, 519, 605
BtsIMutI CAGTG 4 cut(s) 379, 507, 519, 605
Cac8I GCNNGC 6 cut(s) 47, 89, 107, 144, 161, 346
CfoI GCGC 8 cut(s) 76, 127, 142, 161, 163, 182, 205, 214
Cfr10I RCCGGY 2 cut(s) 142, 776
Cfr13I GGNCC 3 cut(s) 328, 494, 690
Csp6I GTAC 7 cut(s) 133, 196, 208, 324, 460, 772, 780
CspAI ACCGGT 1 cut(s) 776
CviAII CATG 2 cut(s) 457, 808
CviQI GTAC 7 cut(s) 133, 196, 208, 324, 460, 772, 780
DdeI CTNAG 4 cut(s) 498, 591, 596, 611
DpnI GATC 3 cut(s) 363, 491, 648
DpnII GATC 3 cut(s) 361, 489, 646
EaeI YGGCCR 1 cut(s) 103
Eam1104I CTCTTC 1 cut(s) 470
EarI CTCTTC 1 cut(s) 470
EciI GGCGGA 1 cut(s) 374
Ecl136II GAGCTC 1 cut(s) 407
Eco24I GRGCYC 1 cut(s) 409
Eco31I GGTCTC 1 cut(s) 661
Eco47I GGWCC 1 cut(s) 328
Eco53kI GAGCTC 1 cut(s) 407
Eco57I CTGAAG 2 cut(s) 415, 512
Eco81I CCTNAGG 1 cut(s) 498
EcoICRI GAGCTC 1 cut(s) 407
EcoNI CCTNNNNNAGG 1 cut(s) 54
EcoRI GAATTC 1 cut(s) 503
EcoRII CCWGG 4 cut(s) 55, 82, 91, 482
EcoT38I GRGCYC 1 cut(s) 409
FaeI CATG 2 cut(s) 460, 811
FaiI YATR 7 cut(s) 156, 418, 458, 702, 798, 809, 838
FatI CATG 2 cut(s) 456, 807
FauI CCCGC 3 cut(s) 27, 239, 267
Fnu4HI GCNGC 9 cut(s) 43, 72, 77, 80, 147, 201, 244, 294, 821
FriOI GRGCYC 1 cut(s) 409
Fsp4HI GCNGC 9 cut(s) 43, 72, 77, 80, 147, 201, 244, 294, 821
GlaI GCGC 8 cut(s) 75, 126, 141, 160, 162, 181, 204, 213
GluI GCNGC 9 cut(s) 43, 72, 77, 80, 147, 201, 244, 294, 821
HaeII RGCGCY 1 cut(s) 143
HaeIII GGCC 5 cut(s) 87, 96, 105, 496, 692
HapII CCGG 7 cut(s) 27, 102, 143, 272, 321, 675, 777
HhaI GCGC 8 cut(s) 76, 127, 142, 161, 163, 182, 205, 214
Hin1II CATG 2 cut(s) 460, 811
Hin6I GCGC 8 cut(s) 74, 125, 140, 159, 161, 180, 203, 212
HinP1I GCGC 8 cut(s) 74, 125, 140, 159, 161, 180, 203, 212
HincII GTYRAC 1 cut(s) 813
HindII GTYRAC 1 cut(s) 813
HinfI GANTC 1 cut(s) 613
HpaII CCGG 7 cut(s) 27, 102, 143, 272, 321, 675, 777
HphI GGTGA 3 cut(s) 218, 460, 624
Hpy166II GTNNAC 2 cut(s) 774, 813
Hpy188I TCNGA 3 cut(s) 354, 426, 531
Hpy188III TCNNGA 2 cut(s) 6, 365
Hpy8I GTNNAC 2 cut(s) 774, 813
Hpy99I CGWCG 3 cut(s) 268, 313, 389
HpyAV CCTTC 1 cut(s) 754
HpyCH4III ACNGT 7 cut(s) 132, 195, 445, 524, 640, 730, 817
HpyCH4V TGCA 7 cut(s) 149, 170, 400, 512, 587, 605, 836
HpyF3I CTNAG 4 cut(s) 498, 591, 596, 611
Hsp92II CATG 2 cut(s) 460, 811
HspAI GCGC 8 cut(s) 74, 125, 140, 159, 161, 180, 203, 212
KroI GCCGGC 1 cut(s) 142
KroNI GCCGGC 1 cut(s) 144
Kzo9I GATC 3 cut(s) 361, 489, 646
LmnI GCTCC 2 cut(s) 137, 274
Lsp1109I GCAGC 5 cut(s) 54, 91, 133, 255, 807
LweI GCATC 2 cut(s) 574, 592
MaeIII GTNAC 1 cut(s) 630
MalI GATC 3 cut(s) 363, 491, 648
MboI GATC 3 cut(s) 361, 489, 646
MboII GAAGA 4 cut(s) 443, 457, 499, 518
MflI RGATCY 1 cut(s) 646
MhlI GDGCHC 2 cut(s) 409, 579
MluCI AATT 4 cut(s) 503, 662, 706, 749
MlyI GAGTC 1 cut(s) 622
MmeI TCCRAC 1 cut(s) 515
MnlI CCTC 9 cut(s) 60, 234, 432, 473, 531, 546, 671, 793, 839
MroNI GCCGGC 1 cut(s) 142
MseI TTAA 3 cut(s) 732, 740, 753
MslI CAYNNNNRTG 2 cut(s) 512, 582
MspA1I CMGCKG 1 cut(s) 246
MspI CCGG 7 cut(s) 27, 102, 143, 272, 321, 675, 777
MspR9I CCNGG 8 cut(s) 27, 57, 84, 93, 102, 273, 321, 484
MvaI CCWGG 4 cut(s) 57, 84, 93, 484
MvnI CGCG 5 cut(s) 74, 161, 203, 212, 293
NaeI GCCGGC 1 cut(s) 144
NciI CCSGG 4 cut(s) 27, 102, 273, 321
NdeII GATC 3 cut(s) 361, 489, 646
NgoMIV GCCGGC 1 cut(s) 142
NlaIII CATG 2 cut(s) 460, 811
NlaIV GGNNCC 4 cut(s) 192, 270, 303, 330
NmuCI GTSAC 1 cut(s) 630
OliI CACNNNNGTG 1 cut(s) 512
PalAI GGCGCGCC 1 cut(s) 159
PauI GCGCGC 1 cut(s) 159
PdiI GCCGGC 1 cut(s) 144
Pfl23II CGTACG 3 cut(s) 132, 195, 207
PinAI ACCGGT 1 cut(s) 776
PkrI GCNGC 9 cut(s) 44, 73, 78, 81, 148, 202, 245, 295, 822
PleI GAGTC 1 cut(s) 621
PpsI GAGTC 1 cut(s) 621
Psp124BI GAGCTC 1 cut(s) 409
Psp6I CCWGG 4 cut(s) 55, 82, 91, 482
PspGI CCWGG 4 cut(s) 55, 82, 91, 482
PspLI CGTACG 3 cut(s) 132, 195, 207
PspN4I GGNNCC 4 cut(s) 192, 270, 303, 330
PspPI GGNCC 3 cut(s) 328, 494, 690
PstI CTGCAG 1 cut(s) 514
PsuI RGATCY 1 cut(s) 646
PteI GCGCGC 1 cut(s) 159
RsaI GTAC 7 cut(s) 134, 197, 209, 325, 461, 773, 781
RsaNI GTAC 7 cut(s) 133, 196, 208, 324, 460, 772, 780
RseI CAYNNNNRTG 2 cut(s) 512, 582
SacI GAGCTC 1 cut(s) 409
SaqAI TTAA 3 cut(s) 732, 740, 753
SatI GCNGC 9 cut(s) 43, 72, 77, 80, 147, 201, 244, 294, 821
Sau3AI GATC 3 cut(s) 361, 489, 646
Sau96I GGNCC 3 cut(s) 328, 494, 690
SchI GAGTC 1 cut(s) 622
ScrFI CCNGG 8 cut(s) 27, 57, 84, 93, 102, 273, 321, 484
SduI GDGCHC 2 cut(s) 409, 579
SetI ASST 9 cut(s) 154, 409, 431, 437, 465, 557, 597, 637, 797
SfaNI GCATC 2 cut(s) 574, 592
SfcI CTRYAG 2 cut(s) 510, 520
SfiI GGCCNNNNNGGCC 2 cut(s) 93, 102
SgsI GGCGCGCC 1 cut(s) 159
SinI GGWCC 1 cut(s) 328
SmiMI CAYNNNNRTG 2 cut(s) 512, 582
SmlI CTYRAG 1 cut(s) 436
SmoI CTYRAG 1 cut(s) 436
Sse9I AATT 4 cut(s) 503, 662, 706, 749
SsiI CCGC 8 cut(s) 20, 72, 77, 201, 246, 260, 293, 359
SspI AATATT 1 cut(s) 546
SstI GAGCTC 1 cut(s) 409
StyD4I CCNGG 8 cut(s) 25, 55, 82, 91, 100, 271, 319, 482
TaaI ACNGT 7 cut(s) 132, 195, 445, 524, 640, 730, 817
TaqI TCGA 4 cut(s) 384, 558, 666, 714
TasI AATT 4 cut(s) 503, 662, 706, 749
TauI GCSGC 4 cut(s) 74, 79, 203, 296
Tru1I TTAA 3 cut(s) 732, 740, 753
Tru9I TTAA 3 cut(s) 732, 740, 753
TscAI CASTG 4 cut(s) 379, 514, 519, 612
TseFI GTSAC 1 cut(s) 630
TseI GCWGC 5 cut(s) 42, 79, 146, 243, 820
Tsp45I GTSAC 1 cut(s) 630
TspDTI ATGAA 4 cut(s) 370, 437, 700, 796
TspGWI ACGGA 5 cut(s) 150, 252, 294, 341, 616
TspRI CASTG 4 cut(s) 379, 514, 519, 612
VpaK11BI GGWCC 1 cut(s) 328
XagI CCTNNNNNAGG 1 cut(s) 54
XapI RAATTY 3 cut(s) 503, 662, 749
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.