Rw3G023130

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0006634
Physical Location & Seq
Reverse (-)
28798647 .. 28800403
1757 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G023130.1

Sequence Viewer

Length: 825 bp
ATGTCCGGATACCCACAACCACCGCCAAACTACGGCTACGGCAGCGCCCCTCCGCCCGGCAAACCCTACCCCGCCTCCCCTCCCTACGGCGCACCTCCTCCCGCCCAACACCAGGCCTCCCCCTACGGAGCTCCCTCCGCGCCTCCCTACGCCGCTCCTCCTCACGGAAGCAGCGGCGGCGGCGCCGGATACGGTGCTCCTTACGCGGCTCCTCCACAACTCGGCGGCGGCGGATACCCGACCCCGGCCCCGGCTCCGGGCTACGGAAGCCCCTTCGGTGCCCTGGTGCCGTCGTCGTTCCCGCCCGGCACGGACCCGAACGTGGTGGCCTGCTTCCAGATTGCTGATCAGGACGGCAGCGGGCTCATCGACGACAAGGAGTTGCAGAAGGCGCTCTCGTCGTACAATCAGAGCTTCAGCTTGAGGACTGTTCATCTTCTCATGTATCTCTTCACCCAGACCAACACCAGAAAGATCGGGCCTAAGGAATTCACCGCGGTGTTTTACAGTCTTCAGAACTGGAGGGGAATATTTGAGAAGTTCGACAGAGACAGGAGTGGGAAAATTGACTCAAATGAGTTGAGAGATGCATTGGGGAGTCTGGGATTTTCCGTCTCACCTGCGGTTTTGGATTTGCTGGTGTCCAAGTTTGATAAGACTGGAGGCCACATGAAGGCCATTGAATATGATAATTTCATCGAGTGTTGTCTTACTGTTAAGGGGTTAACTGAGAAGTTTAAGGAGAAGGACAAAGCATACACTGGTCATGCAACTTTTAGTTACGAGGACTTCATGTTGATTGTTTTGCCTTTCCTCATCGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_6

Protein Analysis

274

Amino Acids

29.18

Weight (kDa)

6.2

Isoelectric Point (pI)

57.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 174 - 234 1.5e-08 EF-hand domain pair
EF-hand_6 PF13405 174 - 202 2.1e-07 EF-hand domain
EF-hand_1 PF00036 175 - 200 2e-06 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 628
Acc36I ACCTGC 1 cut(s) 628
AccB1I GGYRCC 3 cut(s) 182, 278, 286
AccBSI CCGCTC 1 cut(s) 155
AccII CGCG 3 cut(s) 140, 206, 497
AccIII TCCGGA 1 cut(s) 5
AcsI RAATTY 1 cut(s) 488
AcuI CTGAAG 2 cut(s) 400, 497
AcyI GRCGYC 1 cut(s) 183
AfaI GTAC 1 cut(s) 404
AgsI TTSAA 1 cut(s) 683
AjnI CCWGG 2 cut(s) 111, 282
AleI CACNNNNGTG 1 cut(s) 497
AluBI AGCT 3 cut(s) 131, 414, 420
AluI AGCT 3 cut(s) 131, 414, 420
Alw21I GWGCWC 2 cut(s) 133, 199
Alw26I GTCTC 2 cut(s) 543, 619
Aor13HI TCCGGA 1 cut(s) 5
AoxI GGCC 6 cut(s) 114, 246, 327, 479, 664, 675
ApeKI GCWGC 3 cut(s) 42, 171, 357
ApoI RAATTY 1 cut(s) 488
AspLEI GCGC 5 cut(s) 47, 92, 142, 185, 394
AspS9I GGNCC 3 cut(s) 247, 313, 479
AsuC2I CCSGG 5 cut(s) 57, 245, 251, 258, 306
AsuHPI GGTGA 3 cut(s) 445, 484, 609
AvaII GGWCC 1 cut(s) 313
AxyI CCTNAGG 1 cut(s) 483
BaeGI GKGCMC 1 cut(s) 283
BanI GGYRCC 3 cut(s) 182, 278, 286
BanII GRGCYC 2 cut(s) 133, 366
BbsI GAAGAC 1 cut(s) 503
Bbv12I GWGCWC 2 cut(s) 133, 199
BbvI GCAGC 3 cut(s) 54, 183, 369
BceAI ACGGC 5 cut(s) 49, 55, 103, 274, 370
BciT130I CCWGG 2 cut(s) 113, 284
BciVI GTATCC 2 cut(s) 182, 227
BclI TGATCA 1 cut(s) 346
BcnI CCSGG 5 cut(s) 57, 245, 251, 258, 306
BcoDI GTCTC 2 cut(s) 543, 619
BfoI RGCGCY 3 cut(s) 48, 186, 395
BfuAI ACCTGC 1 cut(s) 628
BfuI GTATCC 2 cut(s) 182, 227
Bme1390I CCNGG 7 cut(s) 57, 113, 245, 251, 258, 284, 306
Bme18I GGWCC 1 cut(s) 313
BmgT120I GGNCC 3 cut(s) 247, 313, 479
BmiI GGNNCC 7 cut(s) 184, 210, 249, 255, 280, 288, 315
BmrFI CCNGG 7 cut(s) 57, 113, 245, 251, 258, 284, 306
BmsI GCATC 1 cut(s) 577
BpiI GAAGAC 1 cut(s) 503
BpmI CTGGAG 2 cut(s) 541, 681
BpuEI CTTGAG 1 cut(s) 442
BpuMI CCSGG 5 cut(s) 57, 245, 251, 258, 306
BsaHI GRCGYC 1 cut(s) 183
BsaJI CCNNGG 4 cut(s) 243, 249, 282, 495
BsaWI WCCGGW 1 cut(s) 5
BsaXI ACNNNNNCTCC 4 cut(s) 59, 89, 101, 131
Bse1I ACTGG 3 cut(s) 524, 664, 766
Bse21I CCTNAGG 1 cut(s) 483
BseAI TCCGGA 1 cut(s) 5
BseBI CCWGG 2 cut(s) 113, 284
BseDI CCNNGG 4 cut(s) 243, 249, 282, 495
BseMII CTCAG 1 cut(s) 720
BseNI ACTGG 3 cut(s) 524, 664, 766
BseRI GAGGAG 4 cut(s) 87, 147, 150, 201
BseSI GKGCMC 1 cut(s) 283
BseXI GCAGC 3 cut(s) 54, 183, 369
Bsh1236I CGCG 3 cut(s) 140, 206, 497
BshFI GGCC 6 cut(s) 116, 248, 329, 481, 666, 677
BshNI GGYRCC 3 cut(s) 182, 278, 286
BsiHKAI GWGCWC 2 cut(s) 133, 199
BsiSI CCGG 7 cut(s) 6, 57, 186, 245, 251, 257, 306
BsmAI GTCTC 2 cut(s) 543, 619
BsmBI CGTCTC 1 cut(s) 619
BsnI GGCC 6 cut(s) 116, 248, 329, 481, 666, 677
Bsp1286I GDGCHC 4 cut(s) 133, 199, 283, 366
Bsp13I TCCGGA 1 cut(s) 5
Bsp143I GATC 2 cut(s) 346, 474
BspANI GGCC 6 cut(s) 116, 248, 329, 481, 666, 677
BspCNI CTCAG 1 cut(s) 721
BspEI TCCGGA 1 cut(s) 5
BspFNI CGCG 3 cut(s) 140, 206, 497
BspLI GGNNCC 7 cut(s) 184, 210, 249, 255, 280, 288, 315
BspMI ACCTGC 1 cut(s) 628
BspT107I GGYRCC 3 cut(s) 182, 278, 286
BsrBI CCGCTC 1 cut(s) 155
BsrI ACTGG 3 cut(s) 524, 664, 766
BssECI CCNNGG 4 cut(s) 243, 249, 282, 495
BssMI GATC 2 cut(s) 346, 474
BssNI GRCGYC 1 cut(s) 183
Bst2UI CCWGG 2 cut(s) 113, 284
Bst4CI ACNGT 4 cut(s) 194, 430, 509, 715
Bst6I CTCTTC 1 cut(s) 455
BstACI GRCGYC 1 cut(s) 183
BstC8I GCNNGC 2 cut(s) 331, 362
BstDEI CTNAG 2 cut(s) 483, 729
BstDSI CCRYGG 1 cut(s) 495
BstFNI CGCG 3 cut(s) 140, 206, 497
BstH2I RGCGCY 3 cut(s) 48, 186, 395
BstHHI GCGC 5 cut(s) 47, 92, 142, 185, 394
BstKTI GATC 2 cut(s) 349, 477
BstMAI GTCTC 2 cut(s) 543, 619
BstMBI GATC 2 cut(s) 346, 474
BstMWI GCNNNNNNNGC 7 cut(s) 42, 137, 177, 180, 203, 267, 391
BstNI CCWGG 2 cut(s) 113, 284
BstSCI CCNGG 7 cut(s) 55, 111, 243, 249, 256, 282, 304
BstSLI GKGCMC 1 cut(s) 283
BstUI CGCG 3 cut(s) 140, 206, 497
BstV1I GCAGC 3 cut(s) 54, 183, 369
BstV2I GAAGAC 1 cut(s) 503
Bsu36I CCTNAGG 1 cut(s) 483
BsuI GTATCC 2 cut(s) 182, 227
BsuRI GGCC 6 cut(s) 116, 248, 329, 481, 666, 677
BtgI CCRYGG 1 cut(s) 495
BtgZI GCGATG 1 cut(s) 802
BtsIMutI CAGTG 1 cut(s) 759
BveI ACCTGC 1 cut(s) 628
Cac8I GCNNGC 2 cut(s) 331, 362
CfoI GCGC 5 cut(s) 47, 92, 142, 185, 394
Cfr13I GGNCC 3 cut(s) 247, 313, 479
Cfr42I CCGCGG 1 cut(s) 498
Csp6I GTAC 1 cut(s) 403
CviAII CATG 4 cut(s) 442, 670, 767, 793
CviQI GTAC 1 cut(s) 403
DdeI CTNAG 2 cut(s) 483, 729
DinI GGCGCC 1 cut(s) 184
DpnI GATC 2 cut(s) 348, 476
DpnII GATC 2 cut(s) 346, 474
Eam1104I CTCTTC 1 cut(s) 455
EarI CTCTTC 1 cut(s) 455
EciI GGCGGA 2 cut(s) 42, 246
Ecl136II GAGCTC 1 cut(s) 131
Eco147I AGGCCT 1 cut(s) 116
Eco24I GRGCYC 2 cut(s) 133, 366
Eco47I GGWCC 1 cut(s) 313
Eco53kI GAGCTC 1 cut(s) 131
Eco57I CTGAAG 2 cut(s) 400, 497
Eco81I CCTNAGG 1 cut(s) 483
EcoICRI GAGCTC 1 cut(s) 131
EcoRI GAATTC 1 cut(s) 488
EcoRII CCWGG 2 cut(s) 111, 282
EcoT22I ATGCAT 1 cut(s) 592
EcoT38I GRGCYC 2 cut(s) 133, 366
EgeI GGCGCC 1 cut(s) 184
EheI GGCGCC 1 cut(s) 184
Esp3I CGTCTC 1 cut(s) 619
FaeI CATG 4 cut(s) 445, 673, 770, 796
FaiI YATR 7 cut(s) 443, 671, 687, 757, 768, 794, 823
FatI CATG 4 cut(s) 441, 669, 766, 792
FauI CCCGC 4 cut(s) 79, 109, 309, 353
FbaI TGATCA 1 cut(s) 346
FriOI GRGCYC 2 cut(s) 133, 366
GlaI GCGC 5 cut(s) 46, 91, 141, 184, 393
GsuI CTGGAG 2 cut(s) 541, 681
HaeII RGCGCY 3 cut(s) 48, 186, 395
HaeIII GGCC 6 cut(s) 116, 248, 329, 481, 666, 677
HapII CCGG 7 cut(s) 6, 57, 186, 245, 251, 257, 306
HhaI GCGC 5 cut(s) 47, 92, 142, 185, 394
Hin1I GRCGYC 1 cut(s) 183
Hin1II CATG 4 cut(s) 445, 673, 770, 796
Hin6I GCGC 5 cut(s) 45, 90, 140, 183, 392
HinP1I GCGC 5 cut(s) 45, 90, 140, 183, 392
HincII GTYRAC 1 cut(s) 726
HindII GTYRAC 1 cut(s) 726
HinfI GANTC 2 cut(s) 569, 598
HpaI GTTAAC 1 cut(s) 726
HpaII CCGG 7 cut(s) 6, 57, 186, 245, 251, 257, 306
HphI GGTGA 3 cut(s) 445, 484, 609
Hpy166II GTNNAC 1 cut(s) 726
Hpy188I TCNGA 2 cut(s) 411, 516
Hpy188III TCNNGA 3 cut(s) 6, 337, 350
Hpy8I GTNNAC 1 cut(s) 726
Hpy99I CGWCG 4 cut(s) 295, 298, 374, 403
HpyAV CCTTC 4 cut(s) 283, 382, 667, 739
HpyCH4III ACNGT 4 cut(s) 194, 430, 509, 715
HpyCH4IV ACGT 1 cut(s) 321
HpyCH4V TGCA 3 cut(s) 385, 590, 770
HpyF10VI GCNNNNNNNGC 7 cut(s) 42, 137, 177, 180, 203, 267, 391
HpyF3I CTNAG 2 cut(s) 483, 729
HpySE526I ACGT 1 cut(s) 321
Hsp92I GRCGYC 1 cut(s) 183
Hsp92II CATG 4 cut(s) 445, 673, 770, 796
HspAI GCGC 5 cut(s) 45, 90, 140, 183, 392
KasI GGCGCC 1 cut(s) 182
Kpn2I TCCGGA 1 cut(s) 5
Ksp22I TGATCA 1 cut(s) 346
KspAI GTTAAC 1 cut(s) 726
KspI CCGCGG 1 cut(s) 498
Kzo9I GATC 2 cut(s) 346, 474
LmnI GCTCC 6 cut(s) 128, 136, 160, 202, 214, 259
Lsp1109I GCAGC 3 cut(s) 54, 183, 369
LweI GCATC 1 cut(s) 577
MaeII ACGT 1 cut(s) 321
MaeIII GTNAC 1 cut(s) 779
MalI GATC 2 cut(s) 348, 476
MbiI CCGCTC 1 cut(s) 155
MboI GATC 2 cut(s) 346, 474
MboII GAAGA 3 cut(s) 428, 442, 503
MhlI GDGCHC 4 cut(s) 133, 199, 283, 366
MluCI AATT 3 cut(s) 488, 564, 691
Mly113I GGCGCC 1 cut(s) 183
MlyI GAGTC 2 cut(s) 563, 607
Mph1103I ATGCAT 1 cut(s) 592
MroI TCCGGA 1 cut(s) 5
MseI TTAA 3 cut(s) 717, 725, 738
MslI CAYNNNNRTG 1 cut(s) 497
MspA1I CMGCKG 3 cut(s) 174, 360, 497
MspI CCGG 7 cut(s) 6, 57, 186, 245, 251, 257, 306
MspR9I CCNGG 7 cut(s) 57, 113, 245, 251, 258, 284, 306
MvaI CCWGG 2 cut(s) 113, 284
MvnI CGCG 3 cut(s) 140, 206, 497
MwoI GCNNNNNNNGC 7 cut(s) 42, 137, 177, 180, 203, 267, 391
NarI GGCGCC 1 cut(s) 183
NciI CCSGG 5 cut(s) 57, 245, 251, 258, 306
NdeII GATC 2 cut(s) 346, 474
NlaIII CATG 4 cut(s) 445, 673, 770, 796
NlaIV GGNNCC 7 cut(s) 184, 210, 249, 255, 280, 288, 315
NmeAIII GCCGAG 1 cut(s) 201
NsiI ATGCAT 1 cut(s) 592
OliI CACNNNNGTG 1 cut(s) 497
PaqCI CACCTGC 1 cut(s) 628
PceI AGGCCT 1 cut(s) 116
PleI GAGTC 2 cut(s) 563, 606
PluTI GGCGCC 1 cut(s) 186
PpsI GAGTC 2 cut(s) 563, 606
Psp124BI GAGCTC 1 cut(s) 133
Psp6I CCWGG 2 cut(s) 111, 282
PspGI CCWGG 2 cut(s) 111, 282
PspN4I GGNNCC 7 cut(s) 184, 210, 249, 255, 280, 288, 315
PspPI GGNCC 3 cut(s) 247, 313, 479
RsaI GTAC 1 cut(s) 404
RsaNI GTAC 1 cut(s) 403
RseI CAYNNNNRTG 1 cut(s) 497
SacI GAGCTC 1 cut(s) 133
SacII CCGCGG 1 cut(s) 498
SaqAI TTAA 3 cut(s) 717, 725, 738
Sau3AI GATC 2 cut(s) 346, 474
Sau96I GGNCC 3 cut(s) 247, 313, 479
SchI GAGTC 2 cut(s) 563, 607
ScrFI CCNGG 7 cut(s) 57, 113, 245, 251, 258, 284, 306
SduI GDGCHC 4 cut(s) 133, 199, 283, 366
SetI ASST 6 cut(s) 97, 133, 324, 416, 422, 622
SfaNI GCATC 1 cut(s) 577
SfoI GGCGCC 1 cut(s) 184
Sfr303I CCGCGG 1 cut(s) 498
SgrBI CCGCGG 1 cut(s) 498
SinI GGWCC 1 cut(s) 313
SmiMI CAYNNNNRTG 1 cut(s) 497
SmlI CTYRAG 1 cut(s) 421
SmoI CTYRAG 1 cut(s) 421
Sse9I AATT 3 cut(s) 488, 564, 691
SseBI AGGCCT 1 cut(s) 116
SspDI GGCGCC 1 cut(s) 182
SspI AATATT 1 cut(s) 531
SstI GAGCTC 1 cut(s) 133
StuI AGGCCT 1 cut(s) 116
StyD4I CCNGG 7 cut(s) 55, 111, 243, 249, 256, 282, 304
TaaI ACNGT 4 cut(s) 194, 430, 509, 715
TaiI ACGT 1 cut(s) 324
TaqI TCGA 3 cut(s) 369, 543, 699
TasI AATT 3 cut(s) 488, 564, 691
TauI GCSGC 7 cut(s) 155, 177, 180, 183, 209, 228, 231
Tru1I TTAA 3 cut(s) 717, 725, 738
Tru9I TTAA 3 cut(s) 717, 725, 738
TscAI CASTG 1 cut(s) 766
TseI GCWGC 3 cut(s) 42, 171, 357
TspDTI ATGAA 4 cut(s) 422, 685, 686, 781
TspGWI ACGGA 5 cut(s) 141, 180, 279, 326, 601
TspRI CASTG 1 cut(s) 766
VpaK11BI GGWCC 1 cut(s) 313
XapI RAATTY 1 cut(s) 488
Zsp2I ATGCAT 1 cut(s) 592
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.