pycom14g06630

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0006634
Physical Location & Seq
Forward (+)
6601303 .. 6603713
2411 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g06630.2

Sequence Viewer

Length: 864 bp
ATGTCAGGCTACCCACACCCTCCTCCCGGCTCCGGCTACGGAAGCGCACCGCCAGGCCAAGCCTACGGCGCGCCGCCCCAAGGCCAACCCTACGGCGCCCCACCCCAAGGGCAGCACCAGCACCAGACAGCTCAACCCTACGGTGCTCCGTCTGCACCTTACGGCGTCCCATCCTCCGCACCATACGGCGCTCCAGCGGCACCGTACGGCGCGTCCTATGCACCAGACAAGCCGTACAATCCAGACAAACCTCACAAGAATCAAGGCGGCGGAGGCGGATATCTGGCGCCTGCTCCGGGATACGGCAGCCCGTTCGCGGCTTTGGTTCCGTCGGTGTTCCCGCCGGGCACCGACCCGAACGTCATAGCTTGCTTTCAGCTGGCGGATCAGGACGGCAGCGGCTTCATCGACGACAAGGAGATGCAGAGAGCTCTGTCGTCGTACAATCAAAGCTTCAGCTTGAGGACTGTTCATCTTCTCATGTACCTCTTCACCCAGACCAACACCAGGAAGATCGGGCCCAAGGAATTCGCTGCACTGTTCTACAGTCTTCAAAGTTGGAGGGGAGTGTTTGAGAGGTTCGACCGGGACAGAAGTGGGTTCATTGATGCAAATGAGTTGAGAGATGCACTGCTGAGTCTGGGATTTGCTGTCTCACCTGTAGTTTTAGAACTGCTGGTCTCTAAATTCGACAAGACTGGAGGCAAAAAGAGGGCCATTGAATATGACAATTTCATCGAGTGCTGTCTAACTGTTAAGGGATTAACCGAGAAGTTTAAGGAGAAGGACAAGGCGTACACTGGTATGGGAACTTTCAGTTACGAGGAGTTCATGTTGACTGTCCTGCCATTCCTCATTGCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_6

Protein Analysis

288

Amino Acids

30.96

Weight (kDa)

6.52

Isoelectric Point (pI)

49.31

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 359
AccB1I GGYRCC 4 cut(s) 95, 199, 286, 347
AccII CGCG 3 cut(s) 71, 212, 317
AclWI GGATC 1 cut(s) 393
AcsI RAATTY 2 cut(s) 527, 686
AcuI CTGAAG 1 cut(s) 439
AcyI GRCGYC 3 cut(s) 96, 165, 287
AfaI GTAC 5 cut(s) 206, 236, 443, 485, 797
AfiI CCNNNNNNNGG 8 cut(s) 26, 32, 80, 107, 296, 302, 316, 507
AgsI TTSAA 2 cut(s) 554, 722
AjnI CCWGG 2 cut(s) 52, 506
AluBI AGCT 6 cut(s) 131, 368, 379, 431, 453, 459
AluI AGCT 6 cut(s) 131, 368, 379, 431, 453, 459
Alw21I GWGCWC 2 cut(s) 148, 433
Alw26I GTCTC 2 cut(s) 658, 685
AlwI GGATC 1 cut(s) 393
AoxI GGCC 4 cut(s) 55, 82, 518, 714
ApaI GGGCCC 1 cut(s) 522
ApeKI GCWGC 4 cut(s) 112, 306, 396, 533
ApoI RAATTY 2 cut(s) 527, 686
AscI GGCGCGCC 1 cut(s) 69
AspLEI GCGC 7 cut(s) 47, 71, 73, 98, 191, 212, 289
AspS9I GGNCC 3 cut(s) 518, 519, 714
AsuC2I CCSGG 4 cut(s) 27, 297, 345, 587
AsuHPI GGTGA 2 cut(s) 484, 648
BaeGI GKGCMC 2 cut(s) 350, 522
BanI GGYRCC 4 cut(s) 95, 199, 286, 347
BanII GRGCYC 2 cut(s) 433, 522
BbsI GAAGAC 1 cut(s) 542
Bbv12I GWGCWC 2 cut(s) 148, 433
BbvI GCAGC 4 cut(s) 124, 318, 408, 520
BccI CCATC 1 cut(s) 178
BceAI ACGGC 8 cut(s) 82, 109, 178, 202, 217, 223, 319, 409
BcgI CGANNNNNNTGC 2 cut(s) 295, 329
BciT130I CCWGG 2 cut(s) 54, 508
BciVI GTATCC 1 cut(s) 293
BcnI CCSGG 4 cut(s) 27, 297, 345, 587
BcoDI GTCTC 2 cut(s) 658, 685
BfmI CTRYAG 2 cut(s) 544, 660
BfoI RGCGCY 3 cut(s) 99, 192, 290
BfuI GTATCC 1 cut(s) 293
BisI GCNGC 9 cut(s) 74, 113, 198, 268, 307, 318, 397, 400, 534
BlsI GCNGC 9 cut(s) 75, 114, 199, 269, 308, 319, 398, 401, 535
Bme1390I CCNGG 6 cut(s) 27, 54, 297, 345, 508, 587
BmgT120I GGNCC 3 cut(s) 518, 519, 714
BmiI GGNNCC 7 cut(s) 31, 97, 201, 288, 327, 349, 520
BmrFI CCNGG 6 cut(s) 27, 54, 297, 345, 508, 587
BmsI GCATC 3 cut(s) 411, 598, 616
BpiI GAAGAC 1 cut(s) 542
BpmI CTGGAG 2 cut(s) 177, 720
BpuEI CTTGAG 1 cut(s) 481
BpuMI CCSGG 4 cut(s) 27, 297, 345, 587
BsaHI GRCGYC 3 cut(s) 96, 165, 287
BsaI GGTCTC 1 cut(s) 685
BsaJI CCNNGG 3 cut(s) 79, 106, 522
BsaXI ACNNNNNCTCC 2 cut(s) 818, 848
Bsc4I CCNNNNNNNGG 8 cut(s) 26, 32, 80, 107, 296, 302, 316, 507
Bse1I ACTGG 2 cut(s) 703, 805
Bse3DI GCAATG 1 cut(s) 855
BseBI CCWGG 2 cut(s) 54, 508
BseDI CCNNGG 3 cut(s) 79, 106, 522
BseGI GGATG 1 cut(s) 170
BseLI CCNNNNNNNGG 8 cut(s) 26, 32, 80, 107, 296, 302, 316, 507
BseMI GCAATG 1 cut(s) 855
BseMII CTCAG 1 cut(s) 626
BseNI ACTGG 2 cut(s) 703, 805
BsePI GCGCGC 1 cut(s) 69
BseRI GAGGAG 2 cut(s) 12, 839
BseSI GKGCMC 2 cut(s) 350, 522
BseXI GCAGC 4 cut(s) 124, 318, 408, 520
BsgI GTGCAG 2 cut(s) 138, 519
Bsh1236I CGCG 3 cut(s) 71, 212, 317
Bsh1285I CGRYCG 1 cut(s) 586
BshFI GGCC 4 cut(s) 57, 84, 520, 716
BshNI GGYRCC 4 cut(s) 95, 199, 286, 347
BsiEI CGRYCG 1 cut(s) 586
BsiHKAI GWGCWC 2 cut(s) 148, 433
BsiSI CCGG 5 cut(s) 27, 33, 296, 344, 586
BsiWI CGTACG 1 cut(s) 204
BslFI GGGAC 2 cut(s) 152, 602
BslI CCNNNNNNNGG 8 cut(s) 26, 32, 80, 107, 296, 302, 316, 507
BsmAI GTCTC 2 cut(s) 658, 685
BsmFI GGGAC 2 cut(s) 152, 602
BsnI GGCC 4 cut(s) 57, 84, 520, 716
Bso31I GGTCTC 1 cut(s) 685
Bsp120I GGGCCC 1 cut(s) 518
Bsp1286I GDGCHC 4 cut(s) 148, 350, 433, 522
Bsp143I GATC 2 cut(s) 385, 513
BspANI GGCC 4 cut(s) 57, 84, 520, 716
BspCNI CTCAG 1 cut(s) 627
BspFNI CGCG 3 cut(s) 71, 212, 317
BspLI GGNNCC 7 cut(s) 31, 97, 201, 288, 327, 349, 520
BspPI GGATC 1 cut(s) 393
BspT107I GGYRCC 4 cut(s) 95, 199, 286, 347
BspTNI GGTCTC 1 cut(s) 685
BsrDI GCAATG 1 cut(s) 855
BsrI ACTGG 2 cut(s) 703, 805
BssECI CCNNGG 3 cut(s) 79, 106, 522
BssHII GCGCGC 1 cut(s) 69
BssMI GATC 2 cut(s) 385, 513
BssNI GRCGYC 3 cut(s) 96, 165, 287
BssT1I CCWWGG 3 cut(s) 79, 106, 522
Bst2UI CCWGG 2 cut(s) 54, 508
Bst4CI ACNGT 7 cut(s) 143, 204, 469, 540, 548, 754, 841
Bst6I CTCTTC 1 cut(s) 494
BstACI GRCGYC 3 cut(s) 96, 165, 287
BstC8I GCNNGC 4 cut(s) 71, 291, 370, 381
BstDEI CTNAG 1 cut(s) 635
BstF5I GGATG 1 cut(s) 170
BstFNI CGCG 3 cut(s) 71, 212, 317
BstH2I RGCGCY 3 cut(s) 99, 192, 290
BstHHI GCGC 7 cut(s) 47, 71, 73, 98, 191, 212, 289
BstKTI GATC 2 cut(s) 388, 516
BstMAI GTCTC 2 cut(s) 658, 685
BstMBI GATC 2 cut(s) 385, 513
BstMCI CGRYCG 1 cut(s) 586
BstMWI GCNNNNNNNGC 7 cut(s) 42, 68, 118, 152, 197, 218, 273
BstNI CCWGG 2 cut(s) 54, 508
BstSCI CCNGG 6 cut(s) 25, 52, 295, 343, 506, 585
BstSFI CTRYAG 2 cut(s) 544, 660
BstSLI GKGCMC 2 cut(s) 350, 522
BstUI CGCG 3 cut(s) 71, 212, 317
BstV1I GCAGC 4 cut(s) 124, 318, 408, 520
BstV2I GAAGAC 1 cut(s) 542
BsuI GTATCC 1 cut(s) 293
BsuRI GGCC 4 cut(s) 57, 84, 520, 716
BtsCI GGATG 1 cut(s) 170
BtsI GCAGTG 1 cut(s) 629
BtsIMutI CAGTG 3 cut(s) 536, 629, 798
Cac8I GCNNGC 4 cut(s) 71, 291, 370, 381
CfoI GCGC 7 cut(s) 47, 71, 73, 98, 191, 212, 289
Cfr13I GGNCC 3 cut(s) 518, 519, 714
CseI GACGC 2 cut(s) 154, 201
Csp6I GTAC 5 cut(s) 205, 235, 442, 484, 796
CviAII CATG 2 cut(s) 481, 832
CviQI GTAC 5 cut(s) 205, 235, 442, 484, 796
DdeI CTNAG 1 cut(s) 635
DinI GGCGCC 2 cut(s) 97, 288
DpnI GATC 2 cut(s) 387, 515
DpnII GATC 2 cut(s) 385, 513
DrdI GACNNNNNNGTC 1 cut(s) 359
DseDI GACNNNNNNGTC 1 cut(s) 359
Eam1104I CTCTTC 1 cut(s) 494
EarI CTCTTC 1 cut(s) 494
EciI GGCGGA 3 cut(s) 285, 291, 398
Ecl136II GAGCTC 1 cut(s) 431
Eco130I CCWWGG 3 cut(s) 79, 106, 522
Eco24I GRGCYC 2 cut(s) 433, 522
Eco31I GGTCTC 1 cut(s) 685
Eco32I GATATC 1 cut(s) 281
Eco53kI GAGCTC 1 cut(s) 431
Eco57I CTGAAG 1 cut(s) 439
EcoICRI GAGCTC 1 cut(s) 431
EcoRI GAATTC 1 cut(s) 527
EcoRII CCWGG 2 cut(s) 52, 506
EcoRV GATATC 1 cut(s) 281
EcoT14I CCWWGG 3 cut(s) 79, 106, 522
EcoT38I GRGCYC 2 cut(s) 433, 522
EgeI GGCGCC 2 cut(s) 97, 288
EheI GGCGCC 2 cut(s) 97, 288
ErhI CCWWGG 3 cut(s) 79, 106, 522
FaeI CATG 2 cut(s) 484, 835
FaiI YATR 7 cut(s) 184, 219, 365, 482, 726, 806, 833
FaqI GGGAC 2 cut(s) 152, 602
FatI CATG 2 cut(s) 480, 831
FauI CCCGC 1 cut(s) 348
Fnu4HI GCNGC 9 cut(s) 74, 113, 198, 268, 307, 318, 397, 400, 534
FokI GGATG 1 cut(s) 157
FriOI GRGCYC 2 cut(s) 433, 522
Fsp4HI GCNGC 9 cut(s) 74, 113, 198, 268, 307, 318, 397, 400, 534
GlaI GCGC 7 cut(s) 46, 70, 72, 97, 190, 211, 288
GluI GCNGC 9 cut(s) 74, 113, 198, 268, 307, 318, 397, 400, 534
GsuI CTGGAG 2 cut(s) 177, 720
HaeII RGCGCY 3 cut(s) 99, 192, 290
HaeIII GGCC 4 cut(s) 57, 84, 520, 716
HapII CCGG 5 cut(s) 27, 33, 296, 344, 586
HgaI GACGC 2 cut(s) 154, 201
HhaI GCGC 7 cut(s) 47, 71, 73, 98, 191, 212, 289
Hin1I GRCGYC 3 cut(s) 96, 165, 287
Hin1II CATG 2 cut(s) 484, 835
Hin6I GCGC 7 cut(s) 45, 69, 71, 96, 189, 210, 287
HinP1I GCGC 7 cut(s) 45, 69, 71, 96, 189, 210, 287
HincII GTYRAC 1 cut(s) 837
HindII GTYRAC 1 cut(s) 837
HindIII AAGCTT 1 cut(s) 451
HinfI GANTC 2 cut(s) 259, 637
HpaII CCGG 5 cut(s) 27, 33, 296, 344, 586
HphI GGTGA 2 cut(s) 484, 648
Hpy166II GTNNAC 2 cut(s) 798, 837
Hpy188III TCNNGA 2 cut(s) 242, 389
Hpy8I GTNNAC 2 cut(s) 798, 837
Hpy99I CGWCG 3 cut(s) 334, 413, 442
HpyAV CCTTC 1 cut(s) 778
HpyCH4III ACNGT 7 cut(s) 143, 204, 469, 540, 548, 754, 841
HpyCH4IV ACGT 1 cut(s) 360
HpyCH4V TGCA 6 cut(s) 155, 221, 424, 536, 611, 629
HpyF10VI GCNNNNNNNGC 7 cut(s) 42, 68, 118, 152, 197, 218, 273
HpyF3I CTNAG 1 cut(s) 635
HpySE526I ACGT 1 cut(s) 360
Hsp92I GRCGYC 3 cut(s) 96, 165, 287
Hsp92II CATG 2 cut(s) 484, 835
HspAI GCGC 7 cut(s) 45, 69, 71, 96, 189, 210, 287
KasI GGCGCC 2 cut(s) 95, 286
Kzo9I GATC 2 cut(s) 385, 513
LmnI GCTCC 4 cut(s) 35, 151, 196, 298
Lsp1109I GCAGC 4 cut(s) 124, 318, 408, 520
LweI GCATC 3 cut(s) 411, 598, 616
MaeII ACGT 1 cut(s) 360
MaeIII GTNAC 1 cut(s) 818
MalI GATC 2 cut(s) 387, 515
MboI GATC 2 cut(s) 385, 513
MboII GAAGA 4 cut(s) 467, 481, 523, 542
MhlI GDGCHC 4 cut(s) 148, 350, 433, 522
MluCI AATT 3 cut(s) 527, 686, 730
Mly113I GGCGCC 2 cut(s) 96, 287
MlyI GAGTC 1 cut(s) 646
MmeI TCCRAC 1 cut(s) 539
MseI TTAA 3 cut(s) 756, 764, 777
MslI CAYNNNNRTG 1 cut(s) 803
MspA1I CMGCKG 3 cut(s) 197, 379, 399
MspI CCGG 5 cut(s) 27, 33, 296, 344, 586
MspR9I CCNGG 6 cut(s) 27, 54, 297, 345, 508, 587
MvaI CCWGG 2 cut(s) 54, 508
MvnI CGCG 3 cut(s) 71, 212, 317
MwoI GCNNNNNNNGC 7 cut(s) 42, 68, 118, 152, 197, 218, 273
NarI GGCGCC 2 cut(s) 96, 287
NciI CCSGG 4 cut(s) 27, 297, 345, 587
NdeII GATC 2 cut(s) 385, 513
NlaIII CATG 2 cut(s) 484, 835
NlaIV GGNNCC 7 cut(s) 31, 97, 201, 288, 327, 349, 520
PalAI GGCGCGCC 1 cut(s) 69
PauI GCGCGC 1 cut(s) 69
PfeI GAWTC 1 cut(s) 259
Pfl23II CGTACG 1 cut(s) 204
PfoI TCCNGGA 1 cut(s) 295
PkrI GCNGC 9 cut(s) 75, 114, 199, 269, 308, 319, 398, 401, 535
PleI GAGTC 1 cut(s) 645
PluTI GGCGCC 2 cut(s) 99, 290
PpsI GAGTC 1 cut(s) 645
Psp124BI GAGCTC 1 cut(s) 433
Psp6I CCWGG 2 cut(s) 52, 506
PspGI CCWGG 2 cut(s) 52, 506
PspLI CGTACG 1 cut(s) 204
PspN4I GGNNCC 7 cut(s) 31, 97, 201, 288, 327, 349, 520
PspOMI GGGCCC 1 cut(s) 518
PspPI GGNCC 3 cut(s) 518, 519, 714
PteI GCGCGC 1 cut(s) 69
PvuII CAGCTG 1 cut(s) 379
RsaI GTAC 5 cut(s) 206, 236, 443, 485, 797
RsaNI GTAC 5 cut(s) 205, 235, 442, 484, 796
RseI CAYNNNNRTG 1 cut(s) 803
SacI GAGCTC 1 cut(s) 433
SaqAI TTAA 3 cut(s) 756, 764, 777
SatI GCNGC 9 cut(s) 74, 113, 198, 268, 307, 318, 397, 400, 534
Sau3AI GATC 2 cut(s) 385, 513
Sau96I GGNCC 3 cut(s) 518, 519, 714
SchI GAGTC 1 cut(s) 646
ScrFI CCNGG 6 cut(s) 27, 54, 297, 345, 508, 587
SduI GDGCHC 4 cut(s) 148, 350, 433, 522
SfaNI GCATC 3 cut(s) 411, 598, 616
SfcI CTRYAG 2 cut(s) 544, 660
SfoI GGCGCC 2 cut(s) 97, 288
SgsI GGCGCGCC 1 cut(s) 69
SmiMI CAYNNNNRTG 1 cut(s) 803
SmlI CTYRAG 1 cut(s) 460
SmoI CTYRAG 1 cut(s) 460
Sse9I AATT 3 cut(s) 527, 686, 730
SspDI GGCGCC 2 cut(s) 95, 286
SstI GAGCTC 1 cut(s) 433
StyD4I CCNGG 6 cut(s) 25, 52, 295, 343, 506, 585
StyI CCWWGG 3 cut(s) 79, 106, 522
TaaI ACNGT 7 cut(s) 143, 204, 469, 540, 548, 754, 841
TaiI ACGT 1 cut(s) 363
TaqI TCGA 4 cut(s) 408, 582, 690, 738
TasI AATT 3 cut(s) 527, 686, 730
TauI GCSGC 5 cut(s) 76, 200, 270, 320, 402
TfiI GAWTC 1 cut(s) 259
Tru1I TTAA 3 cut(s) 756, 764, 777
Tru9I TTAA 3 cut(s) 756, 764, 777
TscAI CASTG 3 cut(s) 543, 636, 805
TseI GCWGC 4 cut(s) 112, 306, 396, 533
TspDTI ATGAA 5 cut(s) 394, 461, 592, 724, 820
TspGWI ACGGA 3 cut(s) 54, 138, 318
TspRI CASTG 3 cut(s) 543, 636, 805
XapI RAATTY 2 cut(s) 527, 686
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.