Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0006634

Physical Location & Seq

Genomic Coordinates Forward (+)

10072636 .. 10075256

Gene Structure 2621 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1082900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1005 bp

ATGTCAGGCTACCCACACCCTCCTTCCGGCTACGGCTACGGCAGCGCTCCACCACCAGGCCAACCTTACGGCGCGCCGCCTCAAGGCCAGCCCTACGGTGCGCCGCCCCAAGGTCAACCCTACGGCGCAACGCCGCAAGGGCAGCACCAGACAGCCCAACCCTACAGTGCTCCGTCTGCACCGTACGGCGCTCCATCCTCCGCACCGTACGGTGCTCCGGCAGCACCGTACGGCGCGCCTTACGCGCCAGAGAAGCCTTACAATCCAGACAAGCCTCACAAGAATCAAGGCGGAGGAGGCGGAGGCGGAGGCGGATACCCGAATCAAGGTGGAGGAGGCGGAGGCGGAGGCGGATACCCGAATCAAGGTGGAGGAGGCGGAGGCGGATACCCGAATCAAGGCGGAGGAGGCGGAGGCGGATACCCGCCACCTGCTCATGGTTACGGGAGCCCGTTTGCGGCTTTGTTGCCGTCGGTGTTCCCGCCGGGCACAGACCCGAACGTCATAGCTTGCTTTCAGCTGGCGGATCAGGACGGCAGCGGCTTCATCGACGACAAGGAGATGCAGAGAGCTCTGTCGTCGTACAATGAAAGCTTCAGCTTGAGGACTGTTCATCTTCTCATGTACACCTTCACCCAGACCAACGCCAGGAAGATCGGGCCTAAGGAATTCGCTGCACTGTTCTACAGTCTTCAAAGTTGGAGGGGAATATTTGAGAGGTTTGACCGGGACAGAAGTGGGTTCATTGATGCAAATGAGTTGAGAGATGCACTGCTGAGTCTGGGATTTTCTGTGTCACCTGTAGTTTTGGAACTGCTGGTCTCTAAATTCGACAAGTCTGGAGGCAAAAAGAGGGCCATTGAATATGACAATTTCATCGAGTGCTGTCTGACTGTTAAGGGATTAACTGAGAAGTTTAAGGAGAAGGACAAAGCGTACACTGGTATGGGAACTTTCAATTATGAGGAGTTCATGTTGACTGTCCTGCCATTCCTCATTGCGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko04626 map04626

Pfam Domains

Protein Families

EF-hand_1 EF-hand_5 EF-hand_6

Protein Analysis

335

Amino Acids

34.92

Weight (kDa)

6.21

Isoelectric Point (pI)

55.09

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EF-hand_7	PF13499	171 - 229	5.4e-06	EF-hand domain pair
EF-hand_7	PF13499	234 - 294	1.7e-08	EF-hand domain pair
EF-hand_6	PF13405	234 - 262	2.8e-07	EF-hand domain
EF-hand_1	PF00036	235 - 259	9.4e-06	EF hand domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006634)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G10300 AT3G10300 AT3G10300 AT3G10300 AT3G10300 AT3G10300 AT5G04170
fragaria_vesca	FvH4_6g24530
malus_domestica	MD12G1082900.v1.1 MD14G1061500.v1.1 MD14G1077400.v1.1 MD14G1077700.v1.1
prunus_persica	Prupe.7G053100_v2.0.a1
pyrus_communis	pycom12g06980 pycom14g06630 pycom14g06640
rosa_chinensis	RchiOBHm_Chr3g0483641
rosa_laevigata	RLG00000023262
rosa_multiflora	Rmu_sc0000555.1_g000008
rosa_roxburghii	Rroxscaffold_6G00398160
rosa_rugosa	Rorug03G0207700
rosa_samantha	Rh3AG256100 Rh3BG292800 Rh3CG290500 Rh3DG286400
rosa_wichuraiana	Rw3G023130

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	439
AasI	GACNNNNNNGTC	1 cut(s)	500
Acc36I	ACCTGC	1 cut(s)	439
AccII	CGCG	3 cut(s)	74, 236, 245
AclWI	GGATC	1 cut(s)	534
AcsI	RAATTY	2 cut(s)	668, 827
AcuI	CTGAAG	1 cut(s)	580
AfaI	GTAC	6 cut(s)	185, 209, 230, 584, 626, 938
AfeI	AGCGCT	1 cut(s)	46
AgsI	TTSAA	3 cut(s)	695, 863, 958
AjnI	CCWGG	2 cut(s)	55, 647
AluBI	AGCT	5 cut(s)	509, 520, 572, 594, 600
AluI	AGCT	5 cut(s)	509, 520, 572, 594, 600
Alw21I	GWGCWC	3 cut(s)	172, 217, 574
Alw26I	GTCTC	1 cut(s)	826
AlwI	GGATC	1 cut(s)	534
Aor51HI	AGCGCT	1 cut(s)	46
AoxI	GGCC	4 cut(s)	58, 85, 659, 855
ApeKI	GCWGC	5 cut(s)	42, 142, 221, 537, 674
ApoI	RAATTY	2 cut(s)	668, 827
AscI	GGCGCGCC	2 cut(s)	72, 234
AspLEI	GCGC	9 cut(s)	47, 74, 76, 103, 128, 191, 236, 238, 247
AspS9I	GGNCC	2 cut(s)	659, 855
AsuC2I	CCSGG	2 cut(s)	486, 728
AsuHPI	GGTGA	2 cut(s)	625, 789
AxyI	CCTNAGG	1 cut(s)	663
BaeGI	GKGCMC	1 cut(s)	491
BanII	GRGCYC	2 cut(s)	452, 574
BbsI	GAAGAC	1 cut(s)	683
Bbv12I	GWGCWC	3 cut(s)	172, 217, 574
BbvI	GCAGC	5 cut(s)	54, 154, 233, 549, 661
BccI	CCATC	1 cut(s)	202
BceAI	ACGGC	8 cut(s)	49, 55, 85, 139, 202, 247, 454, 550
BciT130I	CCWGG	2 cut(s)	57, 649
BciVI	GTATCC	4 cut(s)	308, 347, 380, 413
BcnI	CCSGG	2 cut(s)	486, 728
BcoDI	GTCTC	1 cut(s)	826
BfmI	CTRYAG	3 cut(s)	163, 685, 801
BfoI	RGCGCY	2 cut(s)	48, 192
BfuAI	ACCTGC	1 cut(s)	439
BfuI	GTATCC	4 cut(s)	308, 347, 380, 413
BglI	GCCNNNNNGGC	1 cut(s)	139
Bme1390I	CCNGG	4 cut(s)	57, 486, 649, 728
BmgT120I	GGNCC	2 cut(s)	659, 855
BmiI	GGNNCC	1 cut(s)	449
BmrFI	CCNGG	4 cut(s)	57, 486, 649, 728
BmsI	GCATC	3 cut(s)	552, 739, 757
BpiI	GAAGAC	1 cut(s)	683
BpmI	CTGGAG	1 cut(s)	861
BpuEI	CTTGAG	2 cut(s)	66, 622
BpuMI	CCSGG	2 cut(s)	486, 728
BsaI	GGTCTC	1 cut(s)	826
BsaJI	CCNNGG	1 cut(s)	109
BsaXI	ACNNNNNCTCC	2 cut(s)	959, 989
Bse1I	ACTGG	1 cut(s)	946
Bse21I	CCTNAGG	1 cut(s)	663
Bse3DI	GCAATG	1 cut(s)	996
BseBI	CCWGG	2 cut(s)	57, 649
BseDI	CCNNGG	1 cut(s)	109
BseGI	GGATG	1 cut(s)	194
BseMI	GCAATG	1 cut(s)	996
BseMII	CTCAG	2 cut(s)	767, 900
BseNI	ACTGG	1 cut(s)	946
BsePI	GCGCGC	2 cut(s)	72, 234
BseRI	GAGGAG	5 cut(s)	309, 348, 387, 420, 980
BseSI	GKGCMC	1 cut(s)	491
BseXI	GCAGC	5 cut(s)	54, 154, 233, 549, 661
BsgI	GTGCAG	2 cut(s)	162, 660
Bsh1236I	CGCG	3 cut(s)	74, 236, 245
BshFI	GGCC	4 cut(s)	60, 87, 661, 857
BsiHKAI	GWGCWC	3 cut(s)	172, 217, 574
BsiSI	CCGG	4 cut(s)	27, 218, 485, 727
BsiWI	CGTACG	3 cut(s)	183, 207, 228
BslFI	GGGAC	1 cut(s)	743
BsmAI	GTCTC	1 cut(s)	826
BsmFI	GGGAC	1 cut(s)	743
BsnI	GGCC	4 cut(s)	60, 87, 661, 857
Bso31I	GGTCTC	1 cut(s)	826
Bsp1286I	GDGCHC	5 cut(s)	172, 217, 452, 491, 574
Bsp1407I	TGTACA	1 cut(s)	624
Bsp143I	GATC	2 cut(s)	526, 654
BspANI	GGCC	4 cut(s)	60, 87, 661, 857
BspCNI	CTCAG	2 cut(s)	768, 901
BspFNI	CGCG	3 cut(s)	74, 236, 245
BspLI	GGNNCC	1 cut(s)	449
BspMI	ACCTGC	1 cut(s)	439
BspPI	GGATC	1 cut(s)	534
BspTNI	GGTCTC	1 cut(s)	826
BsrDI	GCAATG	1 cut(s)	996
BsrGI	TGTACA	1 cut(s)	624
BsrI	ACTGG	1 cut(s)	946
BssECI	CCNNGG	1 cut(s)	109
BssHII	GCGCGC	2 cut(s)	72, 234
BssMI	GATC	2 cut(s)	526, 654
BssT1I	CCWWGG	1 cut(s)	109
Bst2UI	CCWGG	2 cut(s)	57, 649
BstAUI	TGTACA	1 cut(s)	624
BstC8I	GCNNGC	5 cut(s)	74, 89, 236, 511, 522
BstDEI	CTNAG	3 cut(s)	663, 776, 909
BstF5I	GGATG	1 cut(s)	194
BstFNI	CGCG	3 cut(s)	74, 236, 245
BstH2I	RGCGCY	2 cut(s)	48, 192
BstHHI	GCGC	9 cut(s)	47, 74, 76, 103, 128, 191, 236, 238, 247
BstKTI	GATC	2 cut(s)	529, 657
BstMAI	GTCTC	1 cut(s)	826
BstMBI	GATC	2 cut(s)	526, 654
BstNI	CCWGG	2 cut(s)	57, 649
BstSCI	CCNGG	4 cut(s)	55, 484, 647, 726
BstSFI	CTRYAG	3 cut(s)	163, 685, 801
BstSLI	GKGCMC	1 cut(s)	491
BstUI	CGCG	3 cut(s)	74, 236, 245
BstV1I	GCAGC	5 cut(s)	54, 154, 233, 549, 661
BstV2I	GAAGAC	1 cut(s)	683
Bsu36I	CCTNAGG	1 cut(s)	663
BsuI	GTATCC	4 cut(s)	308, 347, 380, 413
BsuRI	GGCC	4 cut(s)	60, 87, 661, 857
BtsCI	GGATG	1 cut(s)	194
BtsI	GCAGTG	1 cut(s)	770
BtsIMutI	CAGTG	4 cut(s)	172, 677, 770, 939
BveI	ACCTGC	1 cut(s)	439
Cac8I	GCNNGC	5 cut(s)	74, 89, 236, 511, 522
CfoI	GCGC	9 cut(s)	47, 74, 76, 103, 128, 191, 236, 238, 247
Cfr13I	GGNCC	2 cut(s)	659, 855
Csp6I	GTAC	6 cut(s)	184, 208, 229, 583, 625, 937
CviAII	CATG	3 cut(s)	437, 622, 973
CviQI	GTAC	6 cut(s)	184, 208, 229, 583, 625, 937
DdeI	CTNAG	3 cut(s)	663, 776, 909
DpnI	GATC	2 cut(s)	528, 656
DpnII	GATC	2 cut(s)	526, 654
DrdI	GACNNNNNNGTC	1 cut(s)	500
DseDI	GACNNNNNNGTC	1 cut(s)	500
Ecl136II	GAGCTC	1 cut(s)	572
Eco130I	CCWWGG	1 cut(s)	109
Eco24I	GRGCYC	2 cut(s)	452, 574
Eco31I	GGTCTC	1 cut(s)	826
Eco47III	AGCGCT	1 cut(s)	46
Eco53kI	GAGCTC	1 cut(s)	572
Eco57I	CTGAAG	1 cut(s)	580
Eco81I	CCTNAGG	1 cut(s)	663
EcoICRI	GAGCTC	1 cut(s)	572
EcoRI	GAATTC	1 cut(s)	668
EcoRII	CCWGG	2 cut(s)	55, 647
EcoT14I	CCWWGG	1 cut(s)	109
EcoT38I	GRGCYC	2 cut(s)	452, 574
ErhI	CCWWGG	1 cut(s)	109
FaeI	CATG	3 cut(s)	440, 625, 976
FaiI	YATR	7 cut(s)	438, 506, 623, 867, 947, 963, 974
FaqI	GGGAC	1 cut(s)	743
FatI	CATG	3 cut(s)	436, 621, 972
FauI	CCCGC	2 cut(s)	432, 489
FokI	GGATG	1 cut(s)	181
FriOI	GRGCYC	2 cut(s)	452, 574
GlaI	GCGC	9 cut(s)	46, 73, 75, 102, 127, 190, 235, 237, 246
GsuI	CTGGAG	1 cut(s)	861
HaeII	RGCGCY	2 cut(s)	48, 192
HaeIII	GGCC	4 cut(s)	60, 87, 661, 857
HapII	CCGG	4 cut(s)	27, 218, 485, 727
HhaI	GCGC	9 cut(s)	47, 74, 76, 103, 128, 191, 236, 238, 247
Hin1II	CATG	3 cut(s)	440, 625, 976
Hin6I	GCGC	9 cut(s)	45, 72, 74, 101, 126, 189, 234, 236, 245
HinP1I	GCGC	9 cut(s)	45, 72, 74, 101, 126, 189, 234, 236, 245
HincII	GTYRAC	2 cut(s)	116, 978
HindII	GTYRAC	2 cut(s)	116, 978
HindIII	AAGCTT	1 cut(s)	592
HinfI	GANTC	5 cut(s)	283, 322, 361, 394, 778
HpaII	CCGG	4 cut(s)	27, 218, 485, 727
HphI	GGTGA	2 cut(s)	625, 789
Hpy166II	GTNNAC	4 cut(s)	116, 627, 939, 978
Hpy188I	TCNGA	1 cut(s)	891
Hpy188III	TCNNGA	3 cut(s)	266, 530, 840
Hpy8I	GTNNAC	4 cut(s)	116, 627, 939, 978
Hpy99I	CGWCG	3 cut(s)	475, 554, 583
HpyAV	CCTTC	3 cut(s)	33, 640, 919
HpyCH4IV	ACGT	1 cut(s)	501
HpyCH4V	TGCA	5 cut(s)	179, 565, 677, 752, 770
HpyF3I	CTNAG	3 cut(s)	663, 776, 909
HpySE526I	ACGT	1 cut(s)	501
Hsp92II	CATG	3 cut(s)	440, 625, 976
HspAI	GCGC	9 cut(s)	45, 72, 74, 101, 126, 189, 234, 236, 245
Kzo9I	GATC	2 cut(s)	526, 654
LmnI	GCTCC	5 cut(s)	52, 175, 196, 220, 447
Lsp1109I	GCAGC	5 cut(s)	54, 154, 233, 549, 661
LweI	GCATC	3 cut(s)	552, 739, 757
MaeII	ACGT	1 cut(s)	501
MaeIII	GTNAC	2 cut(s)	440, 795
MalI	GATC	2 cut(s)	528, 656
MboI	GATC	2 cut(s)	526, 654
MboII	GAAGA	3 cut(s)	608, 664, 683
MhlI	GDGCHC	5 cut(s)	172, 217, 452, 491, 574
MluCI	AATT	4 cut(s)	668, 827, 871, 958
MlyI	GAGTC	1 cut(s)	787
MmeI	TCCRAC	1 cut(s)	680
MseI	TTAA	3 cut(s)	897, 905, 918
MslI	CAYNNNNRTG	1 cut(s)	944
MspA1I	CMGCKG	2 cut(s)	520, 540
MspI	CCGG	4 cut(s)	27, 218, 485, 727
MspR9I	CCNGG	4 cut(s)	57, 486, 649, 728
MvaI	CCWGG	2 cut(s)	57, 649
MvnI	CGCG	3 cut(s)	74, 236, 245
NciI	CCSGG	2 cut(s)	486, 728
NdeII	GATC	2 cut(s)	526, 654
NlaIII	CATG	3 cut(s)	440, 625, 976
NlaIV	GGNNCC	1 cut(s)	449
NmuCI	GTSAC	1 cut(s)	795
PalAI	GGCGCGCC	2 cut(s)	72, 234
PaqCI	CACCTGC	1 cut(s)	439
PauI	GCGCGC	2 cut(s)	72, 234
PfeI	GAWTC	4 cut(s)	283, 322, 361, 394
Pfl23II	CGTACG	3 cut(s)	183, 207, 228
PleI	GAGTC	1 cut(s)	786
PpsI	GAGTC	1 cut(s)	786
Psp124BI	GAGCTC	1 cut(s)	574
Psp6I	CCWGG	2 cut(s)	55, 647
PspGI	CCWGG	2 cut(s)	55, 647
PspLI	CGTACG	3 cut(s)	183, 207, 228
PspN4I	GGNNCC	1 cut(s)	449
PspPI	GGNCC	2 cut(s)	659, 855
PteI	GCGCGC	2 cut(s)	72, 234
PvuII	CAGCTG	1 cut(s)	520
RsaI	GTAC	6 cut(s)	185, 209, 230, 584, 626, 938
RsaNI	GTAC	6 cut(s)	184, 208, 229, 583, 625, 937
RseI	CAYNNNNRTG	1 cut(s)	944
SacI	GAGCTC	1 cut(s)	574
SaqAI	TTAA	3 cut(s)	897, 905, 918
Sau3AI	GATC	2 cut(s)	526, 654
Sau96I	GGNCC	2 cut(s)	659, 855
SchI	GAGTC	1 cut(s)	787
ScrFI	CCNGG	4 cut(s)	57, 486, 649, 728
SduI	GDGCHC	5 cut(s)	172, 217, 452, 491, 574
SfaNI	GCATC	3 cut(s)	552, 739, 757
SfcI	CTRYAG	3 cut(s)	163, 685, 801
SgsI	GGCGCGCC	2 cut(s)	72, 234
SmiMI	CAYNNNNRTG	1 cut(s)	944
SmlI	CTYRAG	2 cut(s)	81, 601
SmoI	CTYRAG	2 cut(s)	81, 601
Sse9I	AATT	4 cut(s)	668, 827, 871, 958
SspI	AATATT	1 cut(s)	711
SstI	GAGCTC	1 cut(s)	574
StyD4I	CCNGG	4 cut(s)	55, 484, 647, 726
StyI	CCWWGG	1 cut(s)	109
TaiI	ACGT	1 cut(s)	504
TaqI	TCGA	3 cut(s)	549, 831, 879
TasI	AATT	4 cut(s)	668, 827, 871, 958
TatI	WGTACW	1 cut(s)	624
TauI	GCSGC	5 cut(s)	79, 106, 136, 461, 543
TfiI	GAWTC	4 cut(s)	283, 322, 361, 394
Tru1I	TTAA	3 cut(s)	897, 905, 918
Tru9I	TTAA	3 cut(s)	897, 905, 918
TscAI	CASTG	4 cut(s)	172, 684, 777, 946
TseFI	GTSAC	1 cut(s)	795
TseI	GCWGC	5 cut(s)	42, 142, 221, 537, 674
Tsp45I	GTSAC	1 cut(s)	795
TspDTI	ATGAA	6 cut(s)	535, 602, 603, 733, 865, 961
TspGWI	ACGGA	1 cut(s)	162
TspRI	CASTG	4 cut(s)	172, 684, 777, 946
XapI	RAATTY	2 cut(s)	668, 827

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.