Rh3DG286400

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0006634
Physical Location & Seq
Reverse (-)
29269751 .. 29272651
2901 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG286400.1

Sequence Viewer

Length: 840 bp
ATGTCCGGATACCCACAACCACCGCCAAACTACGGCTACGGCAGCGCCCCTCCGCCCGGCAAACCCTACCCCGCCTCCCCTCCCTACGGCGCACCTCCTCCCGCCCAACACCAACACCAACAACACCAGGCCTCCCCCTACGGAGCTCCCTCCGCGCCTCCCTACGCCGCTCCTCCTCACGGAAGCAGCGGCGGCGGCCCCGGATACGGTGCTCCTTACGCGGCTCCTCCACAACACGGCGGCGGCGGATACCCGACCCCGGCCCCGGCTCCGGGCTACGGAAGCCCCTTCGGTGCTCTGGTGCCGTCGTCGTTCCCGCCCGGCACGGACCCGAACGTGGTGGCCTGCTTCCAGATTGCTGATCAGGACGGCAGCGGGCTCATCGACGACAAGGAGTTGCAGAAGGCGCTCTCGTCGTACAATCAGAGCTTCAGCTTGAGGACTGTTCATCTTCTCATGTATCTCTTCACCCAGACCAACACCAGAAAGATCGGGCCAAAGGAATTCACCGCGGTGTTTTACAGTCTTCAGAACTGGAGGGGAATATTTGAGAAGTTCGACAGAGACAGGAGTGGGAAAATTGACTCAAATGAGTTGAGAGATGCATTGGGGAGTCTGGGATTTTCGGTCTCACCTGCGGTTTTGGATTTGCTGGTGTCTAAGTTTGATAAGACTGGAGGCCACATGAAGGCCATTGAATATGATAATTTCATCGAGTGTTGTCTTACTGTTAAGGGATTAACTGAGAAGTTTAAGGAGAAGGACAAAGCATACACTGGTCATGCAACTTTTAGTTACGAGGACTTCATGTTGATTGTTTTGCCTTTCCTCATCGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_6

Protein Analysis

279

Amino Acids

29.89

Weight (kDa)

6.43

Isoelectric Point (pI)

56.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 179 - 239 1.6e-08 EF-hand domain pair
EF-hand_6 PF13405 179 - 207 2.2e-07 EF-hand domain
EF-hand_1 PF00036 180 - 205 2.1e-06 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 643
Acc36I ACCTGC 1 cut(s) 643
AccB1I GGYRCC 1 cut(s) 301
AccBSI CCGCTC 1 cut(s) 170
AccII CGCG 3 cut(s) 155, 221, 512
AccIII TCCGGA 1 cut(s) 5
AcsI RAATTY 1 cut(s) 503
AcuI CTGAAG 2 cut(s) 415, 512
AfaI GTAC 1 cut(s) 419
AgsI TTSAA 1 cut(s) 698
AjnI CCWGG 1 cut(s) 126
AleI CACNNNNGTG 1 cut(s) 512
AluBI AGCT 3 cut(s) 146, 429, 435
AluI AGCT 3 cut(s) 146, 429, 435
Alw21I GWGCWC 3 cut(s) 148, 214, 298
Alw26I GTCTC 2 cut(s) 558, 634
Aor13HI TCCGGA 1 cut(s) 5
AoxI GGCC 7 cut(s) 129, 196, 261, 342, 494, 679, 690
ApeKI GCWGC 3 cut(s) 42, 186, 372
ApoI RAATTY 1 cut(s) 503
AspLEI GCGC 4 cut(s) 47, 92, 157, 409
AspS9I GGNCC 4 cut(s) 197, 262, 328, 494
AsuC2I CCSGG 6 cut(s) 57, 201, 260, 266, 273, 321
AsuHPI GGTGA 3 cut(s) 460, 499, 624
AvaII GGWCC 1 cut(s) 328
BanI GGYRCC 1 cut(s) 301
BanII GRGCYC 2 cut(s) 148, 381
BbsI GAAGAC 1 cut(s) 518
Bbv12I GWGCWC 3 cut(s) 148, 214, 298
BbvI GCAGC 3 cut(s) 54, 198, 384
BceAI ACGGC 6 cut(s) 49, 55, 103, 253, 289, 385
BciT130I CCWGG 1 cut(s) 128
BciVI GTATCC 2 cut(s) 197, 242
BclI TGATCA 1 cut(s) 361
BcnI CCSGG 6 cut(s) 57, 201, 260, 266, 273, 321
BcoDI GTCTC 2 cut(s) 558, 634
BfoI RGCGCY 2 cut(s) 48, 410
BfuAI ACCTGC 1 cut(s) 643
BfuI GTATCC 2 cut(s) 197, 242
Bme1390I CCNGG 7 cut(s) 57, 128, 201, 260, 266, 273, 321
Bme18I GGWCC 1 cut(s) 328
BmgT120I GGNCC 4 cut(s) 197, 262, 328, 494
BmiI GGNNCC 6 cut(s) 199, 225, 264, 270, 303, 330
BmrFI CCNGG 7 cut(s) 57, 128, 201, 260, 266, 273, 321
BmsI GCATC 1 cut(s) 592
BpiI GAAGAC 1 cut(s) 518
BpmI CTGGAG 2 cut(s) 556, 696
BpuEI CTTGAG 1 cut(s) 457
BpuMI CCSGG 6 cut(s) 57, 201, 260, 266, 273, 321
BsaI GGTCTC 1 cut(s) 634
BsaJI CCNNGG 4 cut(s) 199, 258, 264, 510
BsaWI WCCGGW 1 cut(s) 5
BsaXI ACNNNNNCTCC 4 cut(s) 59, 89, 116, 146
Bse1I ACTGG 3 cut(s) 539, 679, 781
BseAI TCCGGA 1 cut(s) 5
BseBI CCWGG 1 cut(s) 128
BseDI CCNNGG 4 cut(s) 199, 258, 264, 510
BseMII CTCAG 1 cut(s) 735
BseNI ACTGG 3 cut(s) 539, 679, 781
BseRI GAGGAG 4 cut(s) 87, 162, 165, 216
BseXI GCAGC 3 cut(s) 54, 198, 384
Bsh1236I CGCG 3 cut(s) 155, 221, 512
BshFI GGCC 7 cut(s) 131, 198, 263, 344, 496, 681, 692
BshNI GGYRCC 1 cut(s) 301
BsiHKAI GWGCWC 3 cut(s) 148, 214, 298
BsiSI CCGG 7 cut(s) 6, 57, 201, 260, 266, 272, 321
BsmAI GTCTC 2 cut(s) 558, 634
BsnI GGCC 7 cut(s) 131, 198, 263, 344, 496, 681, 692
Bso31I GGTCTC 1 cut(s) 634
Bsp1286I GDGCHC 4 cut(s) 148, 214, 298, 381
Bsp13I TCCGGA 1 cut(s) 5
Bsp143I GATC 2 cut(s) 361, 489
BspANI GGCC 7 cut(s) 131, 198, 263, 344, 496, 681, 692
BspCNI CTCAG 1 cut(s) 736
BspEI TCCGGA 1 cut(s) 5
BspFNI CGCG 3 cut(s) 155, 221, 512
BspLI GGNNCC 6 cut(s) 199, 225, 264, 270, 303, 330
BspMI ACCTGC 1 cut(s) 643
BspT107I GGYRCC 1 cut(s) 301
BspTNI GGTCTC 1 cut(s) 634
BsrBI CCGCTC 1 cut(s) 170
BsrI ACTGG 3 cut(s) 539, 679, 781
BssECI CCNNGG 4 cut(s) 199, 258, 264, 510
BssMI GATC 2 cut(s) 361, 489
Bst2UI CCWGG 1 cut(s) 128
Bst4CI ACNGT 4 cut(s) 209, 445, 524, 730
Bst6I CTCTTC 1 cut(s) 470
BstC8I GCNNGC 2 cut(s) 346, 377
BstDEI CTNAG 2 cut(s) 660, 744
BstDSI CCRYGG 1 cut(s) 510
BstFNI CGCG 3 cut(s) 155, 221, 512
BstH2I RGCGCY 2 cut(s) 48, 410
BstHHI GCGC 4 cut(s) 47, 92, 157, 409
BstKTI GATC 2 cut(s) 364, 492
BstMAI GTCTC 2 cut(s) 558, 634
BstMBI GATC 2 cut(s) 361, 489
BstMWI GCNNNNNNNGC 7 cut(s) 42, 152, 192, 195, 218, 282, 406
BstNI CCWGG 1 cut(s) 128
BstSCI CCNGG 7 cut(s) 55, 126, 199, 258, 264, 271, 319
BstUI CGCG 3 cut(s) 155, 221, 512
BstV1I GCAGC 3 cut(s) 54, 198, 384
BstV2I GAAGAC 1 cut(s) 518
BsuI GTATCC 2 cut(s) 197, 242
BsuRI GGCC 7 cut(s) 131, 198, 263, 344, 496, 681, 692
BtgI CCRYGG 1 cut(s) 510
BtgZI GCGATG 1 cut(s) 817
BtsIMutI CAGTG 1 cut(s) 774
BveI ACCTGC 1 cut(s) 643
Cac8I GCNNGC 2 cut(s) 346, 377
CfoI GCGC 4 cut(s) 47, 92, 157, 409
Cfr13I GGNCC 4 cut(s) 197, 262, 328, 494
Cfr42I CCGCGG 1 cut(s) 513
Csp6I GTAC 1 cut(s) 418
CviAII CATG 4 cut(s) 457, 685, 782, 808
CviQI GTAC 1 cut(s) 418
DdeI CTNAG 2 cut(s) 660, 744
DpnI GATC 2 cut(s) 363, 491
DpnII GATC 2 cut(s) 361, 489
Eam1104I CTCTTC 1 cut(s) 470
EarI CTCTTC 1 cut(s) 470
EciI GGCGGA 2 cut(s) 42, 261
Ecl136II GAGCTC 1 cut(s) 146
Eco147I AGGCCT 1 cut(s) 131
Eco24I GRGCYC 2 cut(s) 148, 381
Eco31I GGTCTC 1 cut(s) 634
Eco47I GGWCC 1 cut(s) 328
Eco53kI GAGCTC 1 cut(s) 146
Eco57I CTGAAG 2 cut(s) 415, 512
EcoICRI GAGCTC 1 cut(s) 146
EcoRI GAATTC 1 cut(s) 503
EcoRII CCWGG 1 cut(s) 126
EcoT22I ATGCAT 1 cut(s) 607
EcoT38I GRGCYC 2 cut(s) 148, 381
FaeI CATG 4 cut(s) 460, 688, 785, 811
FaiI YATR 7 cut(s) 458, 686, 702, 772, 783, 809, 838
FatI CATG 4 cut(s) 456, 684, 781, 807
FauI CCCGC 4 cut(s) 79, 109, 324, 368
FbaI TGATCA 1 cut(s) 361
FriOI GRGCYC 2 cut(s) 148, 381
GlaI GCGC 4 cut(s) 46, 91, 156, 408
GsuI CTGGAG 2 cut(s) 556, 696
HaeII RGCGCY 2 cut(s) 48, 410
HaeIII GGCC 7 cut(s) 131, 198, 263, 344, 496, 681, 692
HapII CCGG 7 cut(s) 6, 57, 201, 260, 266, 272, 321
HhaI GCGC 4 cut(s) 47, 92, 157, 409
Hin1II CATG 4 cut(s) 460, 688, 785, 811
Hin6I GCGC 4 cut(s) 45, 90, 155, 407
HinP1I GCGC 4 cut(s) 45, 90, 155, 407
HinfI GANTC 2 cut(s) 584, 613
HpaII CCGG 7 cut(s) 6, 57, 201, 260, 266, 272, 321
HphI GGTGA 3 cut(s) 460, 499, 624
Hpy188I TCNGA 2 cut(s) 426, 531
Hpy188III TCNNGA 3 cut(s) 6, 352, 365
Hpy99I CGWCG 4 cut(s) 310, 313, 389, 418
HpyAV CCTTC 4 cut(s) 298, 397, 682, 754
HpyCH4III ACNGT 4 cut(s) 209, 445, 524, 730
HpyCH4IV ACGT 1 cut(s) 336
HpyCH4V TGCA 3 cut(s) 400, 605, 785
HpyF10VI GCNNNNNNNGC 7 cut(s) 42, 152, 192, 195, 218, 282, 406
HpyF3I CTNAG 2 cut(s) 660, 744
HpySE526I ACGT 1 cut(s) 336
Hsp92II CATG 4 cut(s) 460, 688, 785, 811
HspAI GCGC 4 cut(s) 45, 90, 155, 407
Kpn2I TCCGGA 1 cut(s) 5
Ksp22I TGATCA 1 cut(s) 361
KspI CCGCGG 1 cut(s) 513
Kzo9I GATC 2 cut(s) 361, 489
LmnI GCTCC 6 cut(s) 143, 151, 175, 217, 229, 274
Lsp1109I GCAGC 3 cut(s) 54, 198, 384
LweI GCATC 1 cut(s) 592
MaeII ACGT 1 cut(s) 336
MaeIII GTNAC 1 cut(s) 794
MalI GATC 2 cut(s) 363, 491
MbiI CCGCTC 1 cut(s) 170
MboI GATC 2 cut(s) 361, 489
MboII GAAGA 3 cut(s) 443, 457, 518
MhlI GDGCHC 4 cut(s) 148, 214, 298, 381
MluCI AATT 3 cut(s) 503, 579, 706
MlyI GAGTC 2 cut(s) 578, 622
Mph1103I ATGCAT 1 cut(s) 607
MroI TCCGGA 1 cut(s) 5
MseI TTAA 3 cut(s) 732, 740, 753
MslI CAYNNNNRTG 1 cut(s) 512
MspA1I CMGCKG 3 cut(s) 189, 375, 512
MspI CCGG 7 cut(s) 6, 57, 201, 260, 266, 272, 321
MspR9I CCNGG 7 cut(s) 57, 128, 201, 260, 266, 273, 321
MvaI CCWGG 1 cut(s) 128
MvnI CGCG 3 cut(s) 155, 221, 512
MwoI GCNNNNNNNGC 7 cut(s) 42, 152, 192, 195, 218, 282, 406
NciI CCSGG 6 cut(s) 57, 201, 260, 266, 273, 321
NdeII GATC 2 cut(s) 361, 489
NlaIII CATG 4 cut(s) 460, 688, 785, 811
NlaIV GGNNCC 6 cut(s) 199, 225, 264, 270, 303, 330
NsiI ATGCAT 1 cut(s) 607
OliI CACNNNNGTG 1 cut(s) 512
PaqCI CACCTGC 1 cut(s) 643
PceI AGGCCT 1 cut(s) 131
PleI GAGTC 2 cut(s) 578, 621
PpsI GAGTC 2 cut(s) 578, 621
Psp124BI GAGCTC 1 cut(s) 148
Psp6I CCWGG 1 cut(s) 126
PspGI CCWGG 1 cut(s) 126
PspN4I GGNNCC 6 cut(s) 199, 225, 264, 270, 303, 330
PspPI GGNCC 4 cut(s) 197, 262, 328, 494
RsaI GTAC 1 cut(s) 419
RsaNI GTAC 1 cut(s) 418
RseI CAYNNNNRTG 1 cut(s) 512
SacI GAGCTC 1 cut(s) 148
SacII CCGCGG 1 cut(s) 513
SaqAI TTAA 3 cut(s) 732, 740, 753
Sau3AI GATC 2 cut(s) 361, 489
Sau96I GGNCC 4 cut(s) 197, 262, 328, 494
SchI GAGTC 2 cut(s) 578, 622
ScrFI CCNGG 7 cut(s) 57, 128, 201, 260, 266, 273, 321
SduI GDGCHC 4 cut(s) 148, 214, 298, 381
SetI ASST 6 cut(s) 97, 148, 339, 431, 437, 637
SfaNI GCATC 1 cut(s) 592
Sfr303I CCGCGG 1 cut(s) 513
SgrBI CCGCGG 1 cut(s) 513
SinI GGWCC 1 cut(s) 328
SmiMI CAYNNNNRTG 1 cut(s) 512
SmlI CTYRAG 1 cut(s) 436
SmoI CTYRAG 1 cut(s) 436
Sse9I AATT 3 cut(s) 503, 579, 706
SseBI AGGCCT 1 cut(s) 131
SspI AATATT 1 cut(s) 546
SstI GAGCTC 1 cut(s) 148
StuI AGGCCT 1 cut(s) 131
StyD4I CCNGG 7 cut(s) 55, 126, 199, 258, 264, 271, 319
TaaI ACNGT 4 cut(s) 209, 445, 524, 730
TaiI ACGT 1 cut(s) 339
TaqI TCGA 3 cut(s) 384, 558, 714
TaqII GACCGA 1 cut(s) 616
TasI AATT 3 cut(s) 503, 579, 706
TauI GCSGC 7 cut(s) 170, 192, 195, 198, 224, 243, 246
Tru1I TTAA 3 cut(s) 732, 740, 753
Tru9I TTAA 3 cut(s) 732, 740, 753
TscAI CASTG 1 cut(s) 781
TseI GCWGC 3 cut(s) 42, 186, 372
TspDTI ATGAA 4 cut(s) 437, 700, 701, 796
TspGWI ACGGA 4 cut(s) 156, 195, 294, 341
TspRI CASTG 1 cut(s) 781
VpaK11BI GGWCC 1 cut(s) 328
XapI RAATTY 1 cut(s) 503
Zsp2I ATGCAT 1 cut(s) 607
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.