Rorug03G0207700

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0006634
Physical Location & Seq
Reverse (-)
18435795 .. 18439439
3645 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0207700.1

Sequence Viewer

Length: 1083 bp
ATGTCCTTGAACCATTTCTCTTCCTCCGAGCTTCAAGAAACGCAGTGGTGGAACAACTCGACGACTCAACAAAACCAGCAACAGCCCTCTGAATTCACCATGGACACTTTCCACCCTCAATTGTATCAAAATTACCAGCCCGGCTGGCTCGGCTCCCAAATCGCCCAACAGCCCCCGACCCTCAATTTCAACCTCAACCATCAACACAACGACGCCGCCGACAGAGAATTACAACAGCCAGAGCTTCAGATCGACATGGGGGATGACAAAGAGACCATGTTCGAGAAGCCGCTGACCCCAAGCGACGTCGGCAAGCTCAACCGCCTCGTCATTCCCAAGCAGCACGCCGAGAGGTACTTCCCTCTTGGCGGCGGCAACGGCGGAGAGTCAGGAGGCGAGAAGGGGCTGCTGCTGAGCTTCGAGGATGAGTCCGGAAAGTGCTGGAGGTTCCGGTACTCGTACTGGAACAGCAGCCAAAGCTATGTATTAACCAAAGGGTGGAGCCGCTACGTGAAGGAAAAGCGCCTCGACGCCGGCGACGTCGTTTTGTTCGAGCGGCGCCGAAACGACAGCGAGCGCCTCTTCATTGGGTGGAGGCGCCGTGGGGCGCAAGAGAGCAGCGGCGCTGGTGGTGGGGCTAGTAACACGTCGGCGCAGGTTAGCGGAAGCGGCGGGGGTGCTGGTGCTGGTGTTGAAAGTGGAGGTAGTAGAGCTGGTTGGACCAGGATCTTCTATTCTGCGCCGCAGTCTTATCCTGCGCCCCATCATCACAGTGTGCCATACCCACCTGACTATCTACAACATGCAGCCGCAGCAGCAGCAGGATCTCCTGTTCAGAACCAGACGCCGTCGTCGTCATCTGGCGGCAACTCGAGGATATTGAGGCTGTTTGGGGTGAACTTGGAGTGCCAGCAGCAGGATGATGAGTCCGAACCATCCACACCAGATGGGTCGTCTTTGTCAAGCCAGGGTCCAACCCACCACCAGTTCTATCCTAATCCTACTCATGCTTATTATGGAGACCACAGGGATTTCACTTTCTCCAGAGATGCGAACCCCCCCATGAGAAATCGACGAGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_6

Protein Analysis

360

Amino Acids

40.0

Weight (kDa)

6.11

Isoelectric Point (pI)

61.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 94 - 201 6.2e-27 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 850
AatII GACGTC 2 cut(s) 309, 543
Acc36I ACCTGC 1 cut(s) 646
AccB1I GGYRCC 2 cut(s) 558, 597
AccB7I CCANNNNNTGG 1 cut(s) 498
AccBSI CCGCTC 1 cut(s) 556
AccIII TCCGGA 1 cut(s) 431
AclWI GGATC 2 cut(s) 734, 832
AcsI RAATTY 1 cut(s) 92
AcuI CTGAAG 1 cut(s) 230
AcyI GRCGYC 7 cut(s) 213, 306, 531, 540, 559, 598, 845
AdeI CACNNNGTG 1 cut(s) 775
AfaI GTAC 3 cut(s) 356, 455, 461
AfiI CCNNNNNNNGG 3 cut(s) 368, 498, 916
AflIII ACRYGT 1 cut(s) 645
AgsI TTSAA 4 cut(s) 10, 35, 190, 695
AjiI CACGTC 1 cut(s) 648
AjnI CCWGG 2 cut(s) 722, 966
AloI GAACNNNNNNTCC 2 cut(s) 816, 848
AluBI AGCT 6 cut(s) 31, 244, 316, 417, 480, 713
AluI AGCT 6 cut(s) 31, 244, 316, 417, 480, 713
Alw26I GTCTC 2 cut(s) 266, 1014
AlwI GGATC 2 cut(s) 734, 832
Ama87I CYCGRG 1 cut(s) 871
Aor13HI TCCGGA 1 cut(s) 431
ApoI RAATTY 1 cut(s) 92
Asp700I GAANNNNTTC 1 cut(s) 14
AspLEI GCGC 9 cut(s) 525, 561, 579, 600, 610, 626, 655, 742, 760
AspS9I GGNCC 2 cut(s) 720, 971
AsuC2I CCSGG 1 cut(s) 141
AsuHPI GGTGA 2 cut(s) 88, 907
AvaI CYCGRG 1 cut(s) 871
AvaII GGWCC 2 cut(s) 720, 971
BanI GGYRCC 2 cut(s) 558, 597
BccI CCATC 4 cut(s) 207, 771, 941, 943
BceAI ACGGC 3 cut(s) 394, 585, 832
BciT130I CCWGG 2 cut(s) 724, 968
BcnI CCSGG 1 cut(s) 141
BcoDI GTCTC 2 cut(s) 266, 1014
BfaI CTAG 1 cut(s) 639
BfoI RGCGCY 5 cut(s) 526, 562, 580, 601, 627
BfuAI ACCTGC 1 cut(s) 646
BglI GCCNNNNNGGC 1 cut(s) 145
BlpI GCTNAGC 1 cut(s) 413
Bme1390I CCNGG 3 cut(s) 141, 724, 968
Bme18I GGWCC 2 cut(s) 720, 971
BmeT110I CYCGRG 1 cut(s) 871
BmgBI CACGTC 1 cut(s) 648
BmgT120I GGNCC 2 cut(s) 720, 971
BmiI GGNNCC 6 cut(s) 154, 449, 503, 560, 599, 972
BmrFI CCNGG 3 cut(s) 141, 724, 968
BmsI GCATC 1 cut(s) 1039
BpmI CTGGAG 2 cut(s) 463, 1027
Bpu1102I GCTNAGC 1 cut(s) 413
BpuMI CCSGG 1 cut(s) 141
BsaAI YACGTR 1 cut(s) 511
BsaHI GRCGYC 7 cut(s) 213, 306, 531, 540, 559, 598, 845
BsaI GGTCTC 2 cut(s) 266, 1014
BsaJI CCNNGG 3 cut(s) 99, 601, 967
BsaWI WCCGGW 2 cut(s) 431, 450
BsaXI ACNNNNNCTCC 2 cut(s) 586, 616
Bsc4I CCNNNNNNNGG 3 cut(s) 368, 498, 916
Bse118I RCCGGY 1 cut(s) 533
Bse1I ACTGG 2 cut(s) 467, 985
BseAI TCCGGA 1 cut(s) 431
BseBI CCWGG 2 cut(s) 724, 968
BseDI CCNNGG 3 cut(s) 99, 601, 967
BseGI GGATG 4 cut(s) 268, 430, 925, 935
BseLI CCNNNNNNNGG 3 cut(s) 368, 498, 916
BseMII CTCAG 1 cut(s) 404
BseNI ACTGG 2 cut(s) 467, 985
BshNI GGYRCC 2 cut(s) 558, 597
BsiHKCI CYCGRG 1 cut(s) 871
BsiSI CCGG 4 cut(s) 141, 432, 451, 534
BslI CCNNNNNNNGG 3 cut(s) 368, 498, 916
BsmAI GTCTC 2 cut(s) 266, 1014
Bso31I GGTCTC 2 cut(s) 266, 1014
BsoBI CYCGRG 1 cut(s) 871
Bsp13I TCCGGA 1 cut(s) 431
Bsp143I GATC 3 cut(s) 249, 726, 824
Bsp1720I GCTNAGC 1 cut(s) 413
Bsp19I CCATGG 1 cut(s) 99
BspCNI CTCAG 1 cut(s) 405
BspEI TCCGGA 1 cut(s) 431
BspLI GGNNCC 6 cut(s) 154, 449, 503, 560, 599, 972
BspMI ACCTGC 1 cut(s) 646
BspPI GGATC 2 cut(s) 734, 832
BspT107I GGYRCC 2 cut(s) 558, 597
BspTNI GGTCTC 2 cut(s) 266, 1014
BsrBI CCGCTC 1 cut(s) 556
BsrFI RCCGGY 1 cut(s) 533
BsrI ACTGG 2 cut(s) 467, 985
BssAI RCCGGY 1 cut(s) 533
BssECI CCNNGG 3 cut(s) 99, 601, 967
BssMI GATC 3 cut(s) 249, 726, 824
BssNI GRCGYC 7 cut(s) 213, 306, 531, 540, 559, 598, 845
BssT1I CCWWGG 1 cut(s) 99
Bst2UI CCWGG 2 cut(s) 724, 968
Bst4CI ACNGT 1 cut(s) 773
Bst6I CTCTTC 2 cut(s) 25, 587
BstACI GRCGYC 7 cut(s) 213, 306, 531, 540, 559, 598, 845
BstBAI YACGTR 1 cut(s) 511
BstC8I GCNNGC 6 cut(s) 146, 314, 345, 535, 575, 911
BstDEI CTNAG 1 cut(s) 413
BstDSI CCRYGG 2 cut(s) 99, 601
BstF5I GGATG 4 cut(s) 268, 430, 925, 935
BstH2I RGCGCY 5 cut(s) 526, 562, 580, 601, 627
BstHHI GCGC 9 cut(s) 525, 561, 579, 600, 610, 626, 655, 742, 760
BstKTI GATC 3 cut(s) 252, 729, 827
BstMAI GTCTC 2 cut(s) 266, 1014
BstMBI GATC 3 cut(s) 249, 726, 824
BstMWI GCNNNNNNNGC 9 cut(s) 145, 150, 309, 378, 477, 669, 812, 815, 818
BstNI CCWGG 2 cut(s) 724, 968
BstNSI RCATGY 1 cut(s) 806
BstSCI CCNGG 3 cut(s) 139, 722, 966
BstX2I RGATCY 2 cut(s) 726, 824
BstYI RGATCY 2 cut(s) 726, 824
BtgI CCRYGG 2 cut(s) 99, 601
BtrI CACGTC 1 cut(s) 648
BtsCI GGATG 4 cut(s) 268, 430, 925, 935
BtsI GCAGTG 1 cut(s) 50
BtsIMutI CAGTG 2 cut(s) 50, 778
BveI ACCTGC 1 cut(s) 646
Cac8I GCNNGC 6 cut(s) 146, 314, 345, 535, 575, 911
CfoI GCGC 9 cut(s) 525, 561, 579, 600, 610, 626, 655, 742, 760
Cfr10I RCCGGY 1 cut(s) 533
Cfr13I GGNCC 2 cut(s) 720, 971
CseI GACGC 3 cut(s) 221, 539, 853
Csp6I GTAC 3 cut(s) 355, 454, 460
CviAII CATG 6 cut(s) 100, 256, 277, 803, 1007, 1063
CviQI GTAC 3 cut(s) 355, 454, 460
DdeI CTNAG 1 cut(s) 413
DinI GGCGCC 2 cut(s) 560, 599
DpnI GATC 3 cut(s) 251, 728, 826
DpnII GATC 3 cut(s) 249, 726, 824
DraIII CACNNNGTG 1 cut(s) 775
DrdI GACNNNNNNGTC 1 cut(s) 850
DseDI GACNNNNNNGTC 1 cut(s) 850
Eam1104I CTCTTC 2 cut(s) 25, 587
EarI CTCTTC 2 cut(s) 25, 587
EciI GGCGGA 1 cut(s) 396
Eco130I CCWWGG 1 cut(s) 99
Eco31I GGTCTC 2 cut(s) 266, 1014
Eco47I GGWCC 2 cut(s) 720, 971
Eco57I CTGAAG 1 cut(s) 230
Eco88I CYCGRG 1 cut(s) 871
EcoRI GAATTC 1 cut(s) 92
EcoRII CCWGG 2 cut(s) 722, 966
EcoT14I CCWWGG 1 cut(s) 99
EgeI GGCGCC 2 cut(s) 560, 599
EheI GGCGCC 2 cut(s) 560, 599
ErhI CCWWGG 1 cut(s) 99
FaeI CATG 6 cut(s) 103, 259, 280, 806, 1010, 1066
FaiI YATR 9 cut(s) 101, 257, 278, 483, 781, 804, 1008, 1017, 1064
FatI CATG 6 cut(s) 99, 255, 276, 802, 1006, 1062
FauI CCCGC 1 cut(s) 665
FokI GGATG 4 cut(s) 275, 437, 922, 932
FspBI CTAG 1 cut(s) 639
GlaI GCGC 9 cut(s) 524, 560, 578, 599, 609, 625, 654, 741, 759
GsuI CTGGAG 2 cut(s) 463, 1027
HaeII RGCGCY 5 cut(s) 526, 562, 580, 601, 627
HapII CCGG 4 cut(s) 141, 432, 451, 534
HgaI GACGC 3 cut(s) 221, 539, 853
HhaI GCGC 9 cut(s) 525, 561, 579, 600, 610, 626, 655, 742, 760
Hin1I GRCGYC 7 cut(s) 213, 306, 531, 540, 559, 598, 845
Hin1II CATG 6 cut(s) 103, 259, 280, 806, 1010, 1066
Hin6I GCGC 9 cut(s) 523, 559, 577, 598, 608, 624, 653, 740, 758
HinP1I GCGC 9 cut(s) 523, 559, 577, 598, 608, 624, 653, 740, 758
HinfI GANTC 4 cut(s) 64, 386, 428, 926
HpaII CCGG 4 cut(s) 141, 432, 451, 534
HphI GGTGA 2 cut(s) 88, 907
Hpy166II GTNNAC 1 cut(s) 898
Hpy188I TCNGA 5 cut(s) 28, 91, 249, 837, 931
Hpy188III TCNNGA 5 cut(s) 35, 283, 390, 432, 1044
Hpy8I GTNNAC 1 cut(s) 898
HpyAV CCTTC 2 cut(s) 394, 508
HpyCH4III ACNGT 1 cut(s) 773
HpyCH4IV ACGT 4 cut(s) 306, 510, 540, 647
HpyCH4V TGCA 1 cut(s) 806
HpyF10VI GCNNNNNNNGC 9 cut(s) 145, 150, 309, 378, 477, 669, 812, 815, 818
HpyF3I CTNAG 1 cut(s) 413
HpySE526I ACGT 4 cut(s) 306, 510, 540, 647
Hsp92I GRCGYC 7 cut(s) 213, 306, 531, 540, 559, 598, 845
Hsp92II CATG 6 cut(s) 103, 259, 280, 806, 1010, 1066
HspAI GCGC 9 cut(s) 523, 559, 577, 598, 608, 624, 653, 740, 758
KasI GGCGCC 2 cut(s) 558, 597
Kpn2I TCCGGA 1 cut(s) 431
KroI GCCGGC 1 cut(s) 533
KroNI GCCGGC 1 cut(s) 535
Kzo9I GATC 3 cut(s) 249, 726, 824
LmnI GCTCC 2 cut(s) 158, 501
LweI GCATC 1 cut(s) 1039
MaeI CTAG 1 cut(s) 639
MaeII ACGT 4 cut(s) 306, 510, 540, 647
MaeIII GTNAC 1 cut(s) 641
MalI GATC 3 cut(s) 251, 728, 826
MbiI CCGCTC 1 cut(s) 556
MboI GATC 3 cut(s) 249, 726, 824
MboII GAAGA 3 cut(s) 12, 574, 721
MfeI CAATTG 1 cut(s) 119
MflI RGATCY 2 cut(s) 726, 824
MluCI AATT 5 cut(s) 92, 119, 130, 184, 227
Mly113I GGCGCC 2 cut(s) 559, 598
MlyI GAGTC 4 cut(s) 58, 395, 437, 935
MmeI TCCRAC 2 cut(s) 698, 998
MreI CGCCGGCG 1 cut(s) 533
MroI TCCGGA 1 cut(s) 431
MroNI GCCGGC 1 cut(s) 533
MroXI GAANNNNTTC 1 cut(s) 14
MseI TTAA 1 cut(s) 488
MslI CAYNNNNRTG 1 cut(s) 771
MspA1I CMGCKG 2 cut(s) 292, 621
MspI CCGG 4 cut(s) 141, 432, 451, 534
MspR9I CCNGG 3 cut(s) 141, 724, 968
MunI CAATTG 1 cut(s) 119
MvaI CCWGG 2 cut(s) 724, 968
MwoI GCNNNNNNNGC 9 cut(s) 145, 150, 309, 378, 477, 669, 812, 815, 818
NaeI GCCGGC 1 cut(s) 535
NarI GGCGCC 2 cut(s) 559, 598
NciI CCSGG 1 cut(s) 141
NcoI CCATGG 1 cut(s) 99
NdeII GATC 3 cut(s) 249, 726, 824
NgoMIV GCCGGC 1 cut(s) 533
NlaIII CATG 6 cut(s) 103, 259, 280, 806, 1010, 1066
NlaIV GGNNCC 6 cut(s) 154, 449, 503, 560, 599, 972
NmeAIII GCCGAG 2 cut(s) 129, 373
NspI RCATGY 1 cut(s) 806
PaeR7I CTCGAG 1 cut(s) 871
PcsI WCGNNNNNNNCGW 5 cut(s) 216, 534, 537, 549, 851
PdiI GCCGGC 1 cut(s) 535
PdmI GAANNNNTTC 1 cut(s) 14
PflFI GACNNNGTC 1 cut(s) 847
PflMI CCANNNNNTGG 1 cut(s) 498
PleI GAGTC 4 cut(s) 58, 394, 436, 934
PluTI GGCGCC 2 cut(s) 562, 601
PpsI GAGTC 4 cut(s) 58, 394, 436, 934
Ppu21I YACGTR 1 cut(s) 511
Psp6I CCWGG 2 cut(s) 722, 966
PspGI CCWGG 2 cut(s) 722, 966
PspN4I GGNNCC 6 cut(s) 154, 449, 503, 560, 599, 972
PspPI GGNCC 2 cut(s) 720, 971
PspXI VCTCGAGB 1 cut(s) 871
PsuI RGATCY 2 cut(s) 726, 824
PsyI GACNNNGTC 1 cut(s) 847
RsaI GTAC 3 cut(s) 356, 455, 461
RsaNI GTAC 3 cut(s) 355, 454, 460
RseI CAYNNNNRTG 1 cut(s) 771
SaqAI TTAA 1 cut(s) 488
Sau3AI GATC 3 cut(s) 249, 726, 824
Sau96I GGNCC 2 cut(s) 720, 971
SchI GAGTC 4 cut(s) 58, 395, 437, 935
ScrFI CCNGG 3 cut(s) 141, 724, 968
SfaNI GCATC 1 cut(s) 1039
SfoI GGCGCC 2 cut(s) 560, 599
Sfr274I CTCGAG 1 cut(s) 871
SgrAI CRCCGGYG 1 cut(s) 533
SinI GGWCC 2 cut(s) 720, 971
SlaI CTCGAG 1 cut(s) 871
SmiMI CAYNNNNRTG 1 cut(s) 771
SmlI CTYRAG 1 cut(s) 871
SmoI CTYRAG 1 cut(s) 871
Sse9I AATT 5 cut(s) 92, 119, 130, 184, 227
SspDI GGCGCC 2 cut(s) 558, 597
SspMI CTAG 1 cut(s) 639
StyD4I CCNGG 3 cut(s) 139, 722, 966
StyI CCWWGG 1 cut(s) 99
TaaI ACNGT 1 cut(s) 773
TaiI ACGT 4 cut(s) 309, 513, 543, 650
TaqI TCGA 8 cut(s) 59, 252, 282, 420, 528, 552, 872, 1072
TasI AATT 5 cut(s) 92, 119, 130, 184, 227
Tru1I TTAA 1 cut(s) 488
Tru9I TTAA 1 cut(s) 488
TscAI CASTG 2 cut(s) 50, 778
TspDTI ATGAA 1 cut(s) 574
TspRI CASTG 2 cut(s) 50, 778
Tth111I GACNNNGTC 1 cut(s) 847
Van91I CCANNNNNTGG 1 cut(s) 498
VpaK11BI GGWCC 2 cut(s) 720, 971
XapI RAATTY 1 cut(s) 92
XceI RCATGY 1 cut(s) 806
XhoI CTCGAG 1 cut(s) 871
XmnI GAANNNNTTC 1 cut(s) 14
XspI CTAG 1 cut(s) 639
ZraI GACGTC 2 cut(s) 307, 541
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.