Rroxscaffold_6G00398160

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0006634
Physical Location & Seq
Forward (+)
19985633 .. 19988199
2567 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00398160.1

Sequence Viewer

Length: 846 bp
ATGTCAGGATACCCACAACCACCACCAAACTACGGCTACGGCAGCGCCCCTCCGCCCGGCAAACCCTACGCCGCCTCCCCTCCCTACGGCGCACCTCCTCCCGCCCAACACCAACACCAACACCAACAACACCAGGCCTCCCCCTACGGAGCTCCCTCCGCTCCTCCCTACGCCGCTCCTCCTCACGGAAGCAGCGGCGGCGGCGCCGGATACGGTGCTCCTTACGCGGCTCCTCCTCAACACGGCGGCGGCGGATACCCGACCCCGGCCCCGGCTCCGGGCTACGGAAGCCCCTTCGGTGCTCTGGTGCCGTCGTCGTTCCCGCCCGGTACGGACCCGAACGTGGTGGCCTGCTTCCAGATTGCTGATCAGGACGGCAGCGGGCTCATCGACGACAAGGAGTTGCAGAAGGCGCTCTCGTCGTACAATCAGAGCTTCAGCTTGAGGACTGTTCATCTTCTCATGTATCTCTTCACCCAGACCAACACCAGAAAGATCGGGCCTAAGGAATTCACCGCGGTGTTTTACAGTCTTCAGAACTGGAGGGGAATATTTGAGAAGTTCGACAGAGACAGGAGTGGGAAAATTGACTCAAATGAGTTGAGAGATGCATTGGGGAGTCTGGGATTTTCTGTCTCACCTGCGGTTTTGGATTTGCTGGTGTCTAAGTTTGATAAGACTGGAGGCCACATGAAGGCCATTGAATATGATAATTTCATCGAGTGTTGTCTTACTGTTAAGGGATTAACTGAGAAGTTTAAGGAGAAGGACAAAGCATACACTGGTCATGCAACTTTTAGTTACGAGGACTTCATGTTGATTGTTTTGCCTTTCCTCATCGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_6

Protein Analysis

281

Amino Acids

30.11

Weight (kDa)

6.49

Isoelectric Point (pI)

55.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 181 - 241 1.6e-08 EF-hand domain pair
EF-hand_6 PF13405 181 - 209 2.2e-07 EF-hand domain
EF-hand_1 PF00036 182 - 207 2.1e-06 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 649
Acc36I ACCTGC 1 cut(s) 649
AccB1I GGYRCC 2 cut(s) 203, 307
AccBSI CCGCTC 2 cut(s) 161, 176
AccII CGCG 2 cut(s) 227, 518
AcsI RAATTY 1 cut(s) 509
AcuI CTGAAG 2 cut(s) 421, 518
AcyI GRCGYC 1 cut(s) 204
AfaI GTAC 2 cut(s) 331, 425
AgsI TTSAA 1 cut(s) 704
AjnI CCWGG 1 cut(s) 132
AleI CACNNNNGTG 1 cut(s) 518
AluBI AGCT 3 cut(s) 152, 435, 441
AluI AGCT 3 cut(s) 152, 435, 441
Alw21I GWGCWC 3 cut(s) 154, 220, 304
Alw26I GTCTC 2 cut(s) 564, 640
AoxI GGCC 6 cut(s) 135, 267, 348, 500, 685, 696
ApeKI GCWGC 3 cut(s) 42, 192, 378
ApoI RAATTY 1 cut(s) 509
AspLEI GCGC 4 cut(s) 47, 92, 206, 415
AspS9I GGNCC 3 cut(s) 268, 334, 500
AsuC2I CCSGG 5 cut(s) 57, 266, 272, 279, 327
AsuHPI GGTGA 3 cut(s) 466, 505, 630
AvaII GGWCC 1 cut(s) 334
AxyI CCTNAGG 1 cut(s) 504
BanI GGYRCC 2 cut(s) 203, 307
BanII GRGCYC 2 cut(s) 154, 387
BbsI GAAGAC 1 cut(s) 524
Bbv12I GWGCWC 3 cut(s) 154, 220, 304
BbvI GCAGC 3 cut(s) 54, 204, 390
BceAI ACGGC 6 cut(s) 49, 55, 103, 259, 295, 391
BciT130I CCWGG 1 cut(s) 134
BciVI GTATCC 2 cut(s) 203, 248
BclI TGATCA 1 cut(s) 367
BcnI CCSGG 5 cut(s) 57, 266, 272, 279, 327
BcoDI GTCTC 2 cut(s) 564, 640
BfoI RGCGCY 3 cut(s) 48, 207, 416
BfuAI ACCTGC 1 cut(s) 649
BfuI GTATCC 2 cut(s) 203, 248
Bme1390I CCNGG 6 cut(s) 57, 134, 266, 272, 279, 327
Bme18I GGWCC 1 cut(s) 334
BmgT120I GGNCC 3 cut(s) 268, 334, 500
BmiI GGNNCC 6 cut(s) 205, 231, 270, 276, 309, 336
BmrFI CCNGG 6 cut(s) 57, 134, 266, 272, 279, 327
BmsI GCATC 1 cut(s) 598
BpiI GAAGAC 1 cut(s) 524
BpmI CTGGAG 2 cut(s) 562, 702
BpuEI CTTGAG 1 cut(s) 463
BpuMI CCSGG 5 cut(s) 57, 266, 272, 279, 327
BsaHI GRCGYC 1 cut(s) 204
BsaJI CCNNGG 3 cut(s) 264, 270, 516
BsaXI ACNNNNNCTCC 4 cut(s) 59, 89, 122, 152
Bse1I ACTGG 3 cut(s) 545, 685, 787
Bse21I CCTNAGG 1 cut(s) 504
BseBI CCWGG 1 cut(s) 134
BseDI CCNNGG 3 cut(s) 264, 270, 516
BseMII CTCAG 1 cut(s) 741
BseNI ACTGG 3 cut(s) 545, 685, 787
BseRI GAGGAG 6 cut(s) 87, 153, 168, 171, 222, 225
BseXI GCAGC 3 cut(s) 54, 204, 390
Bsh1236I CGCG 2 cut(s) 227, 518
BshFI GGCC 6 cut(s) 137, 269, 350, 502, 687, 698
BshNI GGYRCC 2 cut(s) 203, 307
BsiHKAI GWGCWC 3 cut(s) 154, 220, 304
BsiSI CCGG 6 cut(s) 57, 207, 266, 272, 278, 327
BsmAI GTCTC 2 cut(s) 564, 640
BsnI GGCC 6 cut(s) 137, 269, 350, 502, 687, 698
Bsp1286I GDGCHC 4 cut(s) 154, 220, 304, 387
Bsp143I GATC 2 cut(s) 367, 495
BspANI GGCC 6 cut(s) 137, 269, 350, 502, 687, 698
BspCNI CTCAG 1 cut(s) 742
BspFNI CGCG 2 cut(s) 227, 518
BspLI GGNNCC 6 cut(s) 205, 231, 270, 276, 309, 336
BspMI ACCTGC 1 cut(s) 649
BspT107I GGYRCC 2 cut(s) 203, 307
BsrBI CCGCTC 2 cut(s) 161, 176
BsrI ACTGG 3 cut(s) 545, 685, 787
BssECI CCNNGG 3 cut(s) 264, 270, 516
BssMI GATC 2 cut(s) 367, 495
BssNI GRCGYC 1 cut(s) 204
Bst2UI CCWGG 1 cut(s) 134
Bst4CI ACNGT 4 cut(s) 215, 451, 530, 736
Bst6I CTCTTC 1 cut(s) 476
BstACI GRCGYC 1 cut(s) 204
BstC8I GCNNGC 2 cut(s) 352, 383
BstDEI CTNAG 3 cut(s) 504, 666, 750
BstDSI CCRYGG 1 cut(s) 516
BstFNI CGCG 2 cut(s) 227, 518
BstH2I RGCGCY 3 cut(s) 48, 207, 416
BstHHI GCGC 4 cut(s) 47, 92, 206, 415
BstKTI GATC 2 cut(s) 370, 498
BstMAI GTCTC 2 cut(s) 564, 640
BstMBI GATC 2 cut(s) 367, 495
BstMWI GCNNNNNNNGC 7 cut(s) 42, 158, 198, 201, 224, 288, 412
BstNI CCWGG 1 cut(s) 134
BstSCI CCNGG 6 cut(s) 55, 132, 264, 270, 277, 325
BstUI CGCG 2 cut(s) 227, 518
BstV1I GCAGC 3 cut(s) 54, 204, 390
BstV2I GAAGAC 1 cut(s) 524
Bsu36I CCTNAGG 1 cut(s) 504
BsuI GTATCC 2 cut(s) 203, 248
BsuRI GGCC 6 cut(s) 137, 269, 350, 502, 687, 698
BtgI CCRYGG 1 cut(s) 516
BtgZI GCGATG 1 cut(s) 823
BtsIMutI CAGTG 1 cut(s) 780
BveI ACCTGC 1 cut(s) 649
Cac8I GCNNGC 2 cut(s) 352, 383
CfoI GCGC 4 cut(s) 47, 92, 206, 415
Cfr13I GGNCC 3 cut(s) 268, 334, 500
Cfr42I CCGCGG 1 cut(s) 519
Csp6I GTAC 2 cut(s) 330, 424
CviAII CATG 4 cut(s) 463, 691, 788, 814
CviQI GTAC 2 cut(s) 330, 424
DdeI CTNAG 3 cut(s) 504, 666, 750
DinI GGCGCC 1 cut(s) 205
DpnI GATC 2 cut(s) 369, 497
DpnII GATC 2 cut(s) 367, 495
Eam1104I CTCTTC 1 cut(s) 476
EarI CTCTTC 1 cut(s) 476
EciI GGCGGA 2 cut(s) 42, 267
Ecl136II GAGCTC 1 cut(s) 152
Eco147I AGGCCT 1 cut(s) 137
Eco24I GRGCYC 2 cut(s) 154, 387
Eco47I GGWCC 1 cut(s) 334
Eco53kI GAGCTC 1 cut(s) 152
Eco57I CTGAAG 2 cut(s) 421, 518
Eco81I CCTNAGG 1 cut(s) 504
EcoICRI GAGCTC 1 cut(s) 152
EcoRI GAATTC 1 cut(s) 509
EcoRII CCWGG 1 cut(s) 132
EcoT22I ATGCAT 1 cut(s) 613
EcoT38I GRGCYC 2 cut(s) 154, 387
EgeI GGCGCC 1 cut(s) 205
EheI GGCGCC 1 cut(s) 205
FaeI CATG 4 cut(s) 466, 694, 791, 817
FaiI YATR 7 cut(s) 464, 692, 708, 778, 789, 815, 844
FatI CATG 4 cut(s) 462, 690, 787, 813
FauI CCCGC 3 cut(s) 109, 330, 374
FbaI TGATCA 1 cut(s) 367
FriOI GRGCYC 2 cut(s) 154, 387
GlaI GCGC 4 cut(s) 46, 91, 205, 414
GsuI CTGGAG 2 cut(s) 562, 702
HaeII RGCGCY 3 cut(s) 48, 207, 416
HaeIII GGCC 6 cut(s) 137, 269, 350, 502, 687, 698
HapII CCGG 6 cut(s) 57, 207, 266, 272, 278, 327
HhaI GCGC 4 cut(s) 47, 92, 206, 415
Hin1I GRCGYC 1 cut(s) 204
Hin1II CATG 4 cut(s) 466, 694, 791, 817
Hin6I GCGC 4 cut(s) 45, 90, 204, 413
HinP1I GCGC 4 cut(s) 45, 90, 204, 413
HinfI GANTC 2 cut(s) 590, 619
HpaII CCGG 6 cut(s) 57, 207, 266, 272, 278, 327
HphI GGTGA 3 cut(s) 466, 505, 630
Hpy188I TCNGA 2 cut(s) 432, 537
Hpy188III TCNNGA 3 cut(s) 6, 358, 371
Hpy99I CGWCG 4 cut(s) 316, 319, 395, 424
HpyAV CCTTC 4 cut(s) 304, 403, 688, 760
HpyCH4III ACNGT 4 cut(s) 215, 451, 530, 736
HpyCH4IV ACGT 1 cut(s) 342
HpyCH4V TGCA 3 cut(s) 406, 611, 791
HpyF10VI GCNNNNNNNGC 7 cut(s) 42, 158, 198, 201, 224, 288, 412
HpyF3I CTNAG 3 cut(s) 504, 666, 750
HpySE526I ACGT 1 cut(s) 342
Hsp92I GRCGYC 1 cut(s) 204
Hsp92II CATG 4 cut(s) 466, 694, 791, 817
HspAI GCGC 4 cut(s) 45, 90, 204, 413
KasI GGCGCC 1 cut(s) 203
Ksp22I TGATCA 1 cut(s) 367
KspI CCGCGG 1 cut(s) 519
Kzo9I GATC 2 cut(s) 367, 495
LmnI GCTCC 7 cut(s) 149, 157, 166, 181, 223, 235, 280
Lsp1109I GCAGC 3 cut(s) 54, 204, 390
LweI GCATC 1 cut(s) 598
MaeII ACGT 1 cut(s) 342
MaeIII GTNAC 1 cut(s) 800
MalI GATC 2 cut(s) 369, 497
MbiI CCGCTC 2 cut(s) 161, 176
MboI GATC 2 cut(s) 367, 495
MboII GAAGA 3 cut(s) 449, 463, 524
MhlI GDGCHC 4 cut(s) 154, 220, 304, 387
MluCI AATT 3 cut(s) 509, 585, 712
Mly113I GGCGCC 1 cut(s) 204
MlyI GAGTC 2 cut(s) 584, 628
Mph1103I ATGCAT 1 cut(s) 613
MseI TTAA 3 cut(s) 738, 746, 759
MslI CAYNNNNRTG 1 cut(s) 518
MspA1I CMGCKG 3 cut(s) 195, 381, 518
MspI CCGG 6 cut(s) 57, 207, 266, 272, 278, 327
MspR9I CCNGG 6 cut(s) 57, 134, 266, 272, 279, 327
MvaI CCWGG 1 cut(s) 134
MvnI CGCG 2 cut(s) 227, 518
MwoI GCNNNNNNNGC 7 cut(s) 42, 158, 198, 201, 224, 288, 412
NarI GGCGCC 1 cut(s) 204
NciI CCSGG 5 cut(s) 57, 266, 272, 279, 327
NdeII GATC 2 cut(s) 367, 495
NlaIII CATG 4 cut(s) 466, 694, 791, 817
NlaIV GGNNCC 6 cut(s) 205, 231, 270, 276, 309, 336
NsiI ATGCAT 1 cut(s) 613
OliI CACNNNNGTG 1 cut(s) 518
PaqCI CACCTGC 1 cut(s) 649
PceI AGGCCT 1 cut(s) 137
PleI GAGTC 2 cut(s) 584, 627
PluTI GGCGCC 1 cut(s) 207
PpsI GAGTC 2 cut(s) 584, 627
Psp124BI GAGCTC 1 cut(s) 154
Psp6I CCWGG 1 cut(s) 132
PspGI CCWGG 1 cut(s) 132
PspN4I GGNNCC 6 cut(s) 205, 231, 270, 276, 309, 336
PspPI GGNCC 3 cut(s) 268, 334, 500
RsaI GTAC 2 cut(s) 331, 425
RsaNI GTAC 2 cut(s) 330, 424
RseI CAYNNNNRTG 1 cut(s) 518
SacI GAGCTC 1 cut(s) 154
SacII CCGCGG 1 cut(s) 519
SaqAI TTAA 3 cut(s) 738, 746, 759
Sau3AI GATC 2 cut(s) 367, 495
Sau96I GGNCC 3 cut(s) 268, 334, 500
SchI GAGTC 2 cut(s) 584, 628
ScrFI CCNGG 6 cut(s) 57, 134, 266, 272, 279, 327
SduI GDGCHC 4 cut(s) 154, 220, 304, 387
SetI ASST 6 cut(s) 97, 154, 345, 437, 443, 643
SfaNI GCATC 1 cut(s) 598
SfoI GGCGCC 1 cut(s) 205
Sfr303I CCGCGG 1 cut(s) 519
SgrBI CCGCGG 1 cut(s) 519
SinI GGWCC 1 cut(s) 334
SmiMI CAYNNNNRTG 1 cut(s) 518
SmlI CTYRAG 1 cut(s) 442
SmoI CTYRAG 1 cut(s) 442
Sse9I AATT 3 cut(s) 509, 585, 712
SseBI AGGCCT 1 cut(s) 137
SspDI GGCGCC 1 cut(s) 203
SspI AATATT 1 cut(s) 552
SstI GAGCTC 1 cut(s) 154
StuI AGGCCT 1 cut(s) 137
StyD4I CCNGG 6 cut(s) 55, 132, 264, 270, 277, 325
TaaI ACNGT 4 cut(s) 215, 451, 530, 736
TaiI ACGT 1 cut(s) 345
TaqI TCGA 3 cut(s) 390, 564, 720
TasI AATT 3 cut(s) 509, 585, 712
TauI GCSGC 8 cut(s) 74, 176, 198, 201, 204, 230, 249, 252
Tru1I TTAA 3 cut(s) 738, 746, 759
Tru9I TTAA 3 cut(s) 738, 746, 759
TscAI CASTG 1 cut(s) 787
TseI GCWGC 3 cut(s) 42, 192, 378
TspDTI ATGAA 4 cut(s) 443, 706, 707, 802
TspGWI ACGGA 4 cut(s) 162, 201, 300, 347
TspRI CASTG 1 cut(s) 787
VpaK11BI GGWCC 1 cut(s) 334
XapI RAATTY 1 cut(s) 509
Zsp2I ATGCAT 1 cut(s) 613
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.