MD14G1077400.v1.1

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0006634
Physical Location & Seq
Forward (+)
8792789 .. 8795254
2466 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1077400.v1.1.491

Sequence Viewer

Length: 813 bp
ATGTCAGGCTACCCACACCTTCCTCCCGGCTCCGGCTATGGAAGTGCTCCGCCAGGTCAACCCTACGGCTCGCCGCCCCAAGGGCAGCACCAACACCAGACAGCTCAACCCTACGGTGCTCCGTCTGCACCGTACGGCGCTCCAGCAGCACCGTACGGCGCGCCCTATGCTCCAGACAAGCCGTACAATCCAGACAAGCCTCACAAGAATCAAGGTGGCGGAGGCGGATATCCGGCTCCTGCTCCGGGATATGGCAGCCCGTTCGCGGCTTTGGTTCCGTCGGTGTTCCCGCCGGGCACGGACCCGAACGTCATAGCTTGCTTTCAGCTGGCGGATCAGGACGGCAGCGGCTTCATCGACGACAAGGAGATGCAGAGAGCTCTGTCGTCGTACAATCAGAGCTTCAGCTTGAGGACTGTTCATCTTCTCATGTACCTCTTCACCCAGACCAACACCAGGAAGATCGGGCCCAAGGAATTCGCTGCACTGTTCTACAGTCTTCAAAGTTGGAGGGGAGTGTTTGAGAGGTTCGACCGGGACAGAAGTGGGTTCATTGATGCAAATGAGTTGAGAGATGCACTGCTGAGTCTGGGATTTGCTGTCTCACCTGTAGTTTTAGAACTGCTGGTCTCTAAATTCGACAAGACTGGAGGCAAAAAGAGGGCCATTGAATATGACAATTTCATCGAGTGCTGTCTGACTGTTAAGGGATTAACCGAGAAGTTTAAGGAGAAGGACAAAGCGTACAATGGTATGGGAACTTTCAGTTACGAGGAGTTCATGTTGACTGTCCTGCCATTCCTCATTGCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_6

Protein Analysis

271

Amino Acids

29.37

Weight (kDa)

6.52

Isoelectric Point (pI)

51.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 107 - 165 1.5e-06 EF-hand domain pair
EF-hand_7 PF13499 170 - 230 1.4e-08 EF-hand domain pair
EF-hand_6 PF13405 170 - 198 3e-07 EF-hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 308
AccII CGCG 2 cut(s) 161, 266
AciI CCGC 8 cut(s) 50, 74, 219, 225, 266, 290, 332, 348
AclWI GGATC 1 cut(s) 342
AcsI RAATTY 2 cut(s) 476, 635
AcuI CTGAAG 1 cut(s) 388
AfaI GTAC 6 cut(s) 134, 155, 185, 392, 434, 746
AfiI CCNNNNNNNGG 6 cut(s) 32, 80, 245, 251, 265, 456
AgsI TTSAA 2 cut(s) 503, 671
AjnI CCWGG 2 cut(s) 52, 455
AluBI AGCT 6 cut(s) 104, 317, 328, 380, 402, 408
AluI AGCT 6 cut(s) 104, 317, 328, 380, 402, 408
Alw21I GWGCWC 3 cut(s) 49, 121, 382
Alw26I GTCTC 2 cut(s) 607, 634
AlwI GGATC 1 cut(s) 342
AoxI GGCC 2 cut(s) 467, 663
ApaI GGGCCC 1 cut(s) 471
ApeKI GCWGC 5 cut(s) 85, 146, 255, 345, 482
ApoI RAATTY 2 cut(s) 476, 635
AscI GGCGCGCC 1 cut(s) 159
AspLEI GCGC 3 cut(s) 140, 161, 163
AspS9I GGNCC 4 cut(s) 301, 467, 468, 663
AsuC2I CCSGG 4 cut(s) 27, 246, 294, 536
AsuHPI GGTGA 2 cut(s) 433, 597
AvaII GGWCC 1 cut(s) 301
BaeGI GKGCMC 2 cut(s) 299, 471
BanII GRGCYC 2 cut(s) 382, 471
BbsI GAAGAC 1 cut(s) 491
Bbv12I GWGCWC 3 cut(s) 49, 121, 382
BbvI GCAGC 5 cut(s) 97, 158, 267, 357, 469
BceAI ACGGC 5 cut(s) 82, 151, 166, 172, 358
BcgI CGANNNNNNTGC 2 cut(s) 244, 278
BciT130I CCWGG 2 cut(s) 54, 457
BcnI CCSGG 4 cut(s) 27, 246, 294, 536
BcoDI GTCTC 2 cut(s) 607, 634
BfmI CTRYAG 2 cut(s) 493, 609
BfoI RGCGCY 1 cut(s) 141
BglI GCCNNNNNGGC 1 cut(s) 82
BisI GCNGC 8 cut(s) 74, 86, 147, 256, 267, 346, 349, 483
BlsI GCNGC 8 cut(s) 75, 87, 148, 257, 268, 347, 350, 484
Bme1390I CCNGG 6 cut(s) 27, 54, 246, 294, 457, 536
Bme18I GGWCC 1 cut(s) 301
BmgT120I GGNCC 4 cut(s) 301, 467, 468, 663
BmiI GGNNCC 5 cut(s) 31, 237, 276, 303, 469
BmrFI CCNGG 6 cut(s) 27, 54, 246, 294, 457, 536
BmsI GCATC 3 cut(s) 360, 547, 565
BpiI GAAGAC 1 cut(s) 491
BpmI CTGGAG 3 cut(s) 126, 156, 669
BpuEI CTTGAG 1 cut(s) 430
BpuMI CCSGG 4 cut(s) 27, 246, 294, 536
BsaI GGTCTC 1 cut(s) 634
BsaJI CCNNGG 2 cut(s) 79, 471
BsaXI ACNNNNNCTCC 2 cut(s) 767, 797
Bsc4I CCNNNNNNNGG 6 cut(s) 32, 80, 245, 251, 265, 456
Bse1I ACTGG 1 cut(s) 652
Bse3DI GCAATG 1 cut(s) 804
BseBI CCWGG 2 cut(s) 54, 457
BseDI CCNNGG 2 cut(s) 79, 471
BseLI CCNNNNNNNGG 6 cut(s) 32, 80, 245, 251, 265, 456
BseMI GCAATG 1 cut(s) 804
BseMII CTCAG 1 cut(s) 575
BseNI ACTGG 1 cut(s) 652
BsePI GCGCGC 1 cut(s) 159
BseRI GAGGAG 1 cut(s) 788
BseSI GKGCMC 2 cut(s) 299, 471
BseXI GCAGC 5 cut(s) 97, 158, 267, 357, 469
BsgI GTGCAG 2 cut(s) 111, 468
Bsh1236I CGCG 2 cut(s) 161, 266
Bsh1285I CGRYCG 1 cut(s) 535
BshFI GGCC 2 cut(s) 469, 665
BsiEI CGRYCG 1 cut(s) 535
BsiHKAI GWGCWC 3 cut(s) 49, 121, 382
BsiSI CCGG 6 cut(s) 27, 33, 233, 245, 293, 535
BsiWI CGTACG 2 cut(s) 132, 153
BslFI GGGAC 1 cut(s) 551
BslI CCNNNNNNNGG 6 cut(s) 32, 80, 245, 251, 265, 456
BsmAI GTCTC 2 cut(s) 607, 634
BsmFI GGGAC 1 cut(s) 551
BsnI GGCC 2 cut(s) 469, 665
Bso31I GGTCTC 1 cut(s) 634
Bsp120I GGGCCC 1 cut(s) 467
Bsp1286I GDGCHC 5 cut(s) 49, 121, 299, 382, 471
Bsp143I GATC 2 cut(s) 334, 462
BspACI CCGC 8 cut(s) 50, 74, 219, 225, 266, 290, 332, 348
BspANI GGCC 2 cut(s) 469, 665
BspCNI CTCAG 1 cut(s) 576
BspFNI CGCG 2 cut(s) 161, 266
BspLI GGNNCC 5 cut(s) 31, 237, 276, 303, 469
BspPI GGATC 1 cut(s) 342
BspTNI GGTCTC 1 cut(s) 634
BsrDI GCAATG 1 cut(s) 804
BsrI ACTGG 1 cut(s) 652
BssECI CCNNGG 2 cut(s) 79, 471
BssHII GCGCGC 1 cut(s) 159
BssMI GATC 2 cut(s) 334, 462
BssT1I CCWWGG 2 cut(s) 79, 471
Bst2UI CCWGG 2 cut(s) 54, 457
Bst4CI ACNGT 8 cut(s) 116, 132, 153, 418, 489, 497, 703, 790
Bst6I CTCTTC 1 cut(s) 443
BstC8I GCNNGC 4 cut(s) 71, 161, 319, 330
BstDEI CTNAG 1 cut(s) 584
BstFNI CGCG 2 cut(s) 161, 266
BstH2I RGCGCY 1 cut(s) 141
BstHHI GCGC 3 cut(s) 140, 161, 163
BstKTI GATC 2 cut(s) 337, 465
BstMAI GTCTC 2 cut(s) 607, 634
BstMBI GATC 2 cut(s) 334, 462
BstMCI CGRYCG 1 cut(s) 535
BstMWI GCNNNNNNNGC 4 cut(s) 82, 125, 146, 167
BstNI CCWGG 2 cut(s) 54, 457
BstSCI CCNGG 6 cut(s) 25, 52, 244, 292, 455, 534
BstSFI CTRYAG 2 cut(s) 493, 609
BstSLI GKGCMC 2 cut(s) 299, 471
BstUI CGCG 2 cut(s) 161, 266
BstV1I GCAGC 5 cut(s) 97, 158, 267, 357, 469
BstV2I GAAGAC 1 cut(s) 491
BsuRI GGCC 2 cut(s) 469, 665
BtsI GCAGTG 1 cut(s) 578
BtsIMutI CAGTG 2 cut(s) 485, 578
Cac8I GCNNGC 4 cut(s) 71, 161, 319, 330
CfoI GCGC 3 cut(s) 140, 161, 163
Cfr13I GGNCC 4 cut(s) 301, 467, 468, 663
Csp6I GTAC 6 cut(s) 133, 154, 184, 391, 433, 745
CviAII CATG 2 cut(s) 430, 781
CviQI GTAC 6 cut(s) 133, 154, 184, 391, 433, 745
DdeI CTNAG 1 cut(s) 584
DpnI GATC 2 cut(s) 336, 464
DpnII GATC 2 cut(s) 334, 462
DrdI GACNNNNNNGTC 1 cut(s) 308
DseDI GACNNNNNNGTC 1 cut(s) 308
Eam1104I CTCTTC 1 cut(s) 443
EarI CTCTTC 1 cut(s) 443
EciI GGCGGA 4 cut(s) 39, 234, 240, 347
Ecl136II GAGCTC 1 cut(s) 380
Eco130I CCWWGG 2 cut(s) 79, 471
Eco24I GRGCYC 2 cut(s) 382, 471
Eco31I GGTCTC 1 cut(s) 634
Eco32I GATATC 1 cut(s) 230
Eco47I GGWCC 1 cut(s) 301
Eco53kI GAGCTC 1 cut(s) 380
Eco57I CTGAAG 1 cut(s) 388
EcoICRI GAGCTC 1 cut(s) 380
EcoRI GAATTC 1 cut(s) 476
EcoRII CCWGG 2 cut(s) 52, 455
EcoRV GATATC 1 cut(s) 230
EcoT14I CCWWGG 2 cut(s) 79, 471
EcoT38I GRGCYC 2 cut(s) 382, 471
ErhI CCWWGG 2 cut(s) 79, 471
FaeI CATG 2 cut(s) 433, 784
FaiI YATR 8 cut(s) 39, 168, 252, 314, 431, 675, 755, 782
FaqI GGGAC 1 cut(s) 551
FatI CATG 2 cut(s) 429, 780
FauI CCCGC 1 cut(s) 297
Fnu4HI GCNGC 8 cut(s) 74, 86, 147, 256, 267, 346, 349, 483
FriOI GRGCYC 2 cut(s) 382, 471
Fsp4HI GCNGC 8 cut(s) 74, 86, 147, 256, 267, 346, 349, 483
GlaI GCGC 3 cut(s) 139, 160, 162
GluI GCNGC 8 cut(s) 74, 86, 147, 256, 267, 346, 349, 483
GsuI CTGGAG 3 cut(s) 126, 156, 669
HaeII RGCGCY 1 cut(s) 141
HaeIII GGCC 2 cut(s) 469, 665
HapII CCGG 6 cut(s) 27, 33, 233, 245, 293, 535
HhaI GCGC 3 cut(s) 140, 161, 163
Hin1II CATG 2 cut(s) 433, 784
Hin6I GCGC 3 cut(s) 138, 159, 161
HinP1I GCGC 3 cut(s) 138, 159, 161
HincII GTYRAC 2 cut(s) 59, 786
HindII GTYRAC 2 cut(s) 59, 786
HinfI GANTC 2 cut(s) 208, 586
HpaII CCGG 6 cut(s) 27, 33, 233, 245, 293, 535
HphI GGTGA 2 cut(s) 433, 597
Hpy166II GTNNAC 2 cut(s) 59, 786
Hpy188I TCNGA 2 cut(s) 399, 699
Hpy188III TCNNGA 3 cut(s) 173, 191, 338
Hpy8I GTNNAC 2 cut(s) 59, 786
Hpy99I CGWCG 3 cut(s) 283, 362, 391
HpyAV CCTTC 2 cut(s) 29, 727
HpyCH4III ACNGT 8 cut(s) 116, 132, 153, 418, 489, 497, 703, 790
HpyCH4IV ACGT 1 cut(s) 309
HpyCH4V TGCA 5 cut(s) 128, 373, 485, 560, 578
HpyF10VI GCNNNNNNNGC 4 cut(s) 82, 125, 146, 167
HpyF3I CTNAG 1 cut(s) 584
HpySE526I ACGT 1 cut(s) 309
Hsp92II CATG 2 cut(s) 433, 784
HspAI GCGC 3 cut(s) 138, 159, 161
Kzo9I GATC 2 cut(s) 334, 462
LmnI GCTCC 7 cut(s) 35, 52, 124, 145, 175, 241, 247
Lsp1109I GCAGC 5 cut(s) 97, 158, 267, 357, 469
LweI GCATC 3 cut(s) 360, 547, 565
MaeII ACGT 1 cut(s) 309
MaeIII GTNAC 1 cut(s) 767
MalI GATC 2 cut(s) 336, 464
MboI GATC 2 cut(s) 334, 462
MboII GAAGA 4 cut(s) 416, 430, 472, 491
MhlI GDGCHC 5 cut(s) 49, 121, 299, 382, 471
MluCI AATT 3 cut(s) 476, 635, 679
MlyI GAGTC 1 cut(s) 595
MmeI TCCRAC 1 cut(s) 488
MseI TTAA 3 cut(s) 705, 713, 726
MspA1I CMGCKG 2 cut(s) 328, 348
MspI CCGG 6 cut(s) 27, 33, 233, 245, 293, 535
MspR9I CCNGG 6 cut(s) 27, 54, 246, 294, 457, 536
MvaI CCWGG 2 cut(s) 54, 457
MvnI CGCG 2 cut(s) 161, 266
MwoI GCNNNNNNNGC 4 cut(s) 82, 125, 146, 167
NciI CCSGG 4 cut(s) 27, 246, 294, 536
NdeII GATC 2 cut(s) 334, 462
NlaIII CATG 2 cut(s) 433, 784
NlaIV GGNNCC 5 cut(s) 31, 237, 276, 303, 469
PalAI GGCGCGCC 1 cut(s) 159
PauI GCGCGC 1 cut(s) 159
PfeI GAWTC 1 cut(s) 208
Pfl23II CGTACG 2 cut(s) 132, 153
PfoI TCCNGGA 1 cut(s) 244
PkrI GCNGC 8 cut(s) 75, 87, 148, 257, 268, 347, 350, 484
PleI GAGTC 1 cut(s) 594
PpsI GAGTC 1 cut(s) 594
Psp124BI GAGCTC 1 cut(s) 382
Psp6I CCWGG 2 cut(s) 52, 455
PspGI CCWGG 2 cut(s) 52, 455
PspLI CGTACG 2 cut(s) 132, 153
PspN4I GGNNCC 5 cut(s) 31, 237, 276, 303, 469
PspOMI GGGCCC 1 cut(s) 467
PspPI GGNCC 4 cut(s) 301, 467, 468, 663
PteI GCGCGC 1 cut(s) 159
PvuII CAGCTG 1 cut(s) 328
RsaI GTAC 6 cut(s) 134, 155, 185, 392, 434, 746
RsaNI GTAC 6 cut(s) 133, 154, 184, 391, 433, 745
SacI GAGCTC 1 cut(s) 382
SaqAI TTAA 3 cut(s) 705, 713, 726
SatI GCNGC 8 cut(s) 74, 86, 147, 256, 267, 346, 349, 483
Sau3AI GATC 2 cut(s) 334, 462
Sau96I GGNCC 4 cut(s) 301, 467, 468, 663
SchI GAGTC 1 cut(s) 595
ScrFI CCNGG 6 cut(s) 27, 54, 246, 294, 457, 536
SduI GDGCHC 5 cut(s) 49, 121, 299, 382, 471
SfaNI GCATC 3 cut(s) 360, 547, 565
SfcI CTRYAG 2 cut(s) 493, 609
SgsI GGCGCGCC 1 cut(s) 159
SinI GGWCC 1 cut(s) 301
SmlI CTYRAG 1 cut(s) 409
SmoI CTYRAG 1 cut(s) 409
Sse9I AATT 3 cut(s) 476, 635, 679
SsiI CCGC 8 cut(s) 50, 74, 219, 225, 266, 290, 332, 348
SstI GAGCTC 1 cut(s) 382
StyD4I CCNGG 6 cut(s) 25, 52, 244, 292, 455, 534
StyI CCWWGG 2 cut(s) 79, 471
TaaI ACNGT 8 cut(s) 116, 132, 153, 418, 489, 497, 703, 790
TaiI ACGT 1 cut(s) 312
TaqI TCGA 4 cut(s) 357, 531, 639, 687
TasI AATT 3 cut(s) 476, 635, 679
TauI GCSGC 3 cut(s) 76, 269, 351
TfiI GAWTC 1 cut(s) 208
Tru1I TTAA 3 cut(s) 705, 713, 726
Tru9I TTAA 3 cut(s) 705, 713, 726
TscAI CASTG 2 cut(s) 492, 585
TseI GCWGC 5 cut(s) 85, 146, 255, 345, 482
TspDTI ATGAA 5 cut(s) 343, 410, 541, 673, 769
TspGWI ACGGA 3 cut(s) 111, 267, 314
TspRI CASTG 2 cut(s) 492, 585
VpaK11BI GGWCC 1 cut(s) 301
XapI RAATTY 2 cut(s) 476, 635
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.