pycom14g06640

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0006634
Physical Location & Seq
Forward (+)
6619000 .. 6620754
1755 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g06640.1

Sequence Viewer

Length: 444 bp
ATGCAGAGAGCTATGTCGTCGTACAATCAGAGATTCAGCTTGAGGACTGTTCATCTTCTCATGTACCTTTTCACCCGGACCAACACCAGGAAGATCGGGCCCAAGGAATTCGCTGCACTGTTCTACAGTCTTAAGAGTTGGAGGGGAGCGTTTGAGAGGTTCGACCGGGACAGAAGTGGGTTCATTGATGCAAATGAGTTGAGAGATGCACTGCTGAGTCTGGGATTTGCTGTCTCACCTGTAGTTTTAGAACTGCTGGTCTATAAATTTGGCAAGACTGGAGGCAAAAAGAGGGCCATTGAATATGACAAATTCATCGAGTGCTGTCTGACTGTTAAGGGATTAACTGAGAAGTTTAAGGAGAAGGACAAAGCGTACACCGGTGTGGGAACTTTCACTTATGAGGAGTTCATGTTGACTGTCCTGCCATTCCTCATTGCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_6

Protein Analysis

148

Amino Acids

17.02

Weight (kDa)

9.57

Isoelectric Point (pI)

36.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_6 PF13405 47 - 75 6.8e-08 EF-hand domain
EF-hand_7 PF13499 47 - 107 1.4e-06 EF-hand domain pair
EF-hand_1 PF00036 48 - 72 3.9e-06 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 3 cut(s) 107, 266, 311
AfaI GTAC 3 cut(s) 23, 65, 377
AfiI CCNNNNNNNGG 1 cut(s) 87
AflII CTTAAG 1 cut(s) 131
AgeI ACCGGT 1 cut(s) 380
AgsI TTSAA 1 cut(s) 302
AjnI CCWGG 1 cut(s) 86
AleI CACNNNNGTG 1 cut(s) 383
AluBI AGCT 2 cut(s) 11, 39
AluI AGCT 2 cut(s) 11, 39
Alw26I GTCTC 1 cut(s) 238
AoxI GGCC 2 cut(s) 98, 294
ApaI GGGCCC 1 cut(s) 102
ApeKI GCWGC 1 cut(s) 113
ApoI RAATTY 3 cut(s) 107, 266, 311
AsiGI ACCGGT 1 cut(s) 380
AspS9I GGNCC 4 cut(s) 78, 98, 99, 294
AsuC2I CCSGG 2 cut(s) 76, 167
AsuHPI GGTGA 2 cut(s) 64, 228
AvaII GGWCC 1 cut(s) 78
BaeGI GKGCMC 1 cut(s) 102
BanII GRGCYC 1 cut(s) 102
BbvI GCAGC 1 cut(s) 100
BciT130I CCWGG 1 cut(s) 88
BcnI CCSGG 2 cut(s) 76, 167
BcoDI GTCTC 1 cut(s) 238
BfmI CTRYAG 2 cut(s) 124, 240
BfrI CTTAAG 1 cut(s) 131
BisI GCNGC 1 cut(s) 114
BlsI GCNGC 1 cut(s) 115
Bme1390I CCNGG 3 cut(s) 76, 88, 167
Bme18I GGWCC 1 cut(s) 78
BmgT120I GGNCC 4 cut(s) 78, 98, 99, 294
BmiI GGNNCC 1 cut(s) 100
BmrFI CCNGG 3 cut(s) 76, 88, 167
BmsI GCATC 2 cut(s) 178, 196
BpmI CTGGAG 1 cut(s) 300
BpuEI CTTGAG 1 cut(s) 61
BpuMI CCSGG 2 cut(s) 76, 167
BsaJI CCNNGG 1 cut(s) 102
BsaWI WCCGGW 1 cut(s) 380
BsaXI ACNNNNNCTCC 2 cut(s) 398, 428
Bsc4I CCNNNNNNNGG 1 cut(s) 87
Bse118I RCCGGY 1 cut(s) 380
Bse1I ACTGG 1 cut(s) 283
Bse3DI GCAATG 1 cut(s) 435
BseBI CCWGG 1 cut(s) 88
BseDI CCNNGG 1 cut(s) 102
BseLI CCNNNNNNNGG 1 cut(s) 87
BseMI GCAATG 1 cut(s) 435
BseMII CTCAG 2 cut(s) 206, 339
BseNI ACTGG 1 cut(s) 283
BseRI GAGGAG 1 cut(s) 419
BseSI GKGCMC 1 cut(s) 102
BseXI GCAGC 1 cut(s) 100
BsgI GTGCAG 1 cut(s) 99
Bsh1285I CGRYCG 1 cut(s) 166
BshFI GGCC 2 cut(s) 100, 296
BshTI ACCGGT 1 cut(s) 380
BsiEI CGRYCG 1 cut(s) 166
BsiSI CCGG 3 cut(s) 76, 166, 381
BslFI GGGAC 1 cut(s) 182
BslI CCNNNNNNNGG 1 cut(s) 87
BsmAI GTCTC 1 cut(s) 238
BsmFI GGGAC 1 cut(s) 182
BsnI GGCC 2 cut(s) 100, 296
Bsp120I GGGCCC 1 cut(s) 98
Bsp1286I GDGCHC 1 cut(s) 102
Bsp143I GATC 1 cut(s) 93
BspANI GGCC 2 cut(s) 100, 296
BspCNI CTCAG 2 cut(s) 207, 340
BspLI GGNNCC 1 cut(s) 100
BspTI CTTAAG 1 cut(s) 131
BsrDI GCAATG 1 cut(s) 435
BsrFI RCCGGY 1 cut(s) 380
BsrI ACTGG 1 cut(s) 283
BssAI RCCGGY 1 cut(s) 380
BssECI CCNNGG 1 cut(s) 102
BssMI GATC 1 cut(s) 93
BssT1I CCWWGG 1 cut(s) 102
Bst2UI CCWGG 1 cut(s) 88
Bst4CI ACNGT 5 cut(s) 49, 120, 128, 334, 421
BstAFI CTTAAG 1 cut(s) 131
BstDEI CTNAG 2 cut(s) 215, 348
BstKTI GATC 1 cut(s) 96
BstMAI GTCTC 1 cut(s) 238
BstMBI GATC 1 cut(s) 93
BstMCI CGRYCG 1 cut(s) 166
BstNI CCWGG 1 cut(s) 88
BstSCI CCNGG 3 cut(s) 74, 86, 165
BstSFI CTRYAG 2 cut(s) 124, 240
BstSLI GKGCMC 1 cut(s) 102
BstV1I GCAGC 1 cut(s) 100
BsuRI GGCC 2 cut(s) 100, 296
BtsI GCAGTG 1 cut(s) 209
BtsIMutI CAGTG 2 cut(s) 116, 209
Cfr10I RCCGGY 1 cut(s) 380
Cfr13I GGNCC 4 cut(s) 78, 98, 99, 294
Csp6I GTAC 3 cut(s) 22, 64, 376
CspAI ACCGGT 1 cut(s) 380
CviAII CATG 2 cut(s) 61, 412
CviJI RGCY 4 cut(s) 11, 39, 100, 296
CviKI_1 RGCY 4 cut(s) 11, 39, 100, 296
CviQI GTAC 3 cut(s) 22, 64, 376
DdeI CTNAG 2 cut(s) 215, 348
DpnI GATC 1 cut(s) 95
DpnII GATC 1 cut(s) 93
Eco130I CCWWGG 1 cut(s) 102
Eco24I GRGCYC 1 cut(s) 102
Eco47I GGWCC 1 cut(s) 78
EcoRI GAATTC 1 cut(s) 107
EcoRII CCWGG 1 cut(s) 86
EcoT14I CCWWGG 1 cut(s) 102
EcoT38I GRGCYC 1 cut(s) 102
ErhI CCWWGG 1 cut(s) 102
FaeI CATG 2 cut(s) 64, 415
FaiI YATR 6 cut(s) 14, 62, 264, 306, 402, 413
FaqI GGGAC 1 cut(s) 182
FatI CATG 2 cut(s) 60, 411
Fnu4HI GCNGC 1 cut(s) 114
FriOI GRGCYC 1 cut(s) 102
Fsp4HI GCNGC 1 cut(s) 114
GluI GCNGC 1 cut(s) 114
GsuI CTGGAG 1 cut(s) 300
HaeIII GGCC 2 cut(s) 100, 296
HapII CCGG 3 cut(s) 76, 166, 381
Hin1II CATG 2 cut(s) 64, 415
HincII GTYRAC 1 cut(s) 417
HindII GTYRAC 1 cut(s) 417
HinfI GANTC 2 cut(s) 33, 217
HpaII CCGG 3 cut(s) 76, 166, 381
HphI GGTGA 2 cut(s) 64, 228
Hpy166II GTNNAC 2 cut(s) 378, 417
Hpy188I TCNGA 2 cut(s) 30, 330
Hpy8I GTNNAC 2 cut(s) 378, 417
Hpy99I CGWCG 1 cut(s) 22
HpyAV CCTTC 1 cut(s) 358
HpyCH4III ACNGT 5 cut(s) 49, 120, 128, 334, 421
HpyCH4V TGCA 4 cut(s) 4, 116, 191, 209
HpyF3I CTNAG 2 cut(s) 215, 348
Hsp92II CATG 2 cut(s) 64, 415
Kzo9I GATC 1 cut(s) 93
LmnI GCTCC 1 cut(s) 146
Lsp1109I GCAGC 1 cut(s) 100
LweI GCATC 2 cut(s) 178, 196
MalI GATC 1 cut(s) 95
MboI GATC 1 cut(s) 93
MboII GAAGA 2 cut(s) 47, 103
MhlI GDGCHC 1 cut(s) 102
MluCI AATT 3 cut(s) 107, 266, 311
MlyI GAGTC 1 cut(s) 226
MmeI TCCRAC 1 cut(s) 119
MnlI CCTC 7 cut(s) 36, 135, 150, 275, 285, 397, 443
MseI TTAA 4 cut(s) 132, 336, 344, 357
MslI CAYNNNNRTG 1 cut(s) 383
MspCI CTTAAG 1 cut(s) 131
MspI CCGG 3 cut(s) 76, 166, 381
MspR9I CCNGG 3 cut(s) 76, 88, 167
MvaI CCWGG 1 cut(s) 88
NciI CCSGG 2 cut(s) 76, 167
NdeII GATC 1 cut(s) 93
NlaIII CATG 2 cut(s) 64, 415
NlaIV GGNNCC 1 cut(s) 100
OliI CACNNNNGTG 1 cut(s) 383
PfeI GAWTC 1 cut(s) 33
PinAI ACCGGT 1 cut(s) 380
PkrI GCNGC 1 cut(s) 115
PleI GAGTC 1 cut(s) 225
PpsI GAGTC 1 cut(s) 225
Psp6I CCWGG 1 cut(s) 86
PspGI CCWGG 1 cut(s) 86
PspN4I GGNNCC 1 cut(s) 100
PspOMI GGGCCC 1 cut(s) 98
PspPI GGNCC 4 cut(s) 78, 98, 99, 294
RsaI GTAC 3 cut(s) 23, 65, 377
RsaNI GTAC 3 cut(s) 22, 64, 376
RseI CAYNNNNRTG 1 cut(s) 383
SaqAI TTAA 4 cut(s) 132, 336, 344, 357
SatI GCNGC 1 cut(s) 114
Sau3AI GATC 1 cut(s) 93
Sau96I GGNCC 4 cut(s) 78, 98, 99, 294
SchI GAGTC 1 cut(s) 226
ScrFI CCNGG 3 cut(s) 76, 88, 167
SduI GDGCHC 1 cut(s) 102
SetI ASST 5 cut(s) 13, 41, 69, 161, 241
SfaNI GCATC 2 cut(s) 178, 196
SfcI CTRYAG 2 cut(s) 124, 240
SgrAI CRCCGGYG 1 cut(s) 380
SinI GGWCC 1 cut(s) 78
SmiMI CAYNNNNRTG 1 cut(s) 383
SmlI CTYRAG 2 cut(s) 40, 131
SmoI CTYRAG 2 cut(s) 40, 131
Sse9I AATT 3 cut(s) 107, 266, 311
StyD4I CCNGG 3 cut(s) 74, 86, 165
StyI CCWWGG 1 cut(s) 102
TaaI ACNGT 5 cut(s) 49, 120, 128, 334, 421
TaqI TCGA 2 cut(s) 162, 318
TasI AATT 3 cut(s) 107, 266, 311
TfiI GAWTC 1 cut(s) 33
Tru1I TTAA 4 cut(s) 132, 336, 344, 357
Tru9I TTAA 4 cut(s) 132, 336, 344, 357
TscAI CASTG 2 cut(s) 123, 216
TseI GCWGC 1 cut(s) 113
TspDTI ATGAA 4 cut(s) 41, 172, 304, 400
TspRI CASTG 2 cut(s) 123, 216
Vha464I CTTAAG 1 cut(s) 131
VpaK11BI GGWCC 1 cut(s) 78
XapI RAATTY 3 cut(s) 107, 266, 311
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.