Rh3BG292800

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0006634
Physical Location & Seq
Reverse (-)
29363927 .. 29366100
2174 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG292800.1

Sequence Viewer

Length: 621 bp
ATGTCCGGATACCCACAACCACCGCCAAACTACGGCTACGGCAGCGCCCCTCCGCCCGGCAAACCCTACGCCGCCTCCCCTCCCTACGGCGCACCTCCTCCTCCCGCCCAACACCAACACCAACAACACCAGGCCTCCTCCCCCTACGGAGCTCCCTCCGCGCCCTACGCCGCTCCTCCTCACGGAAGCAGCGGCGGCGCCGCCGCCGGATACGGTGCTCCGTACGCGGCTCCTCCACAACACGGCGGCGGCGGATACCCGACCCCGGCCTCGGCTCCGGCTCCTGGCTACGGAAGCCCCTTTGGTGCTCTGGTGCCGTCGTCGTTCCCGCCCGGTACGGACCCGAACGTGGTGGCTTGCTTTCAGATTGCTGATCAGGACGGCAGCGGGTTGATCGACGACAAGGAGTTGCAGAAGGCGCTCTCGTCGTACAATCAGAGCTTCAGCTTGAGGACTGTTCATCTTCTCATGTATCTCTTCACCCAGACCAACACCAGAAAGATCGGGCCTAAGGAATTCACTGCTGTGTTTTACAGTCTTCAGAACTGGAGGGTAATTTTTTTTTTTTTTTTTGTTGAAATGCTTTGTACTTTTGTGTGTGTGAAATTTGGATTTACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_6

Protein Analysis

206

Amino Acids

21.74

Weight (kDa)

7.73

Isoelectric Point (pI)

60.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 120 - 177 6.6e-06 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 197, 313
AccBSI CCGCTC 1 cut(s) 173
AccII CGCG 2 cut(s) 161, 227
AccIII TCCGGA 1 cut(s) 5
AcsI RAATTY 2 cut(s) 515, 605
AcuI CTGAAG 2 cut(s) 427, 524
AcyI GRCGYC 1 cut(s) 198
AfaI GTAC 4 cut(s) 224, 337, 431, 589
AgsI TTSAA 1 cut(s) 578
AjnI CCWGG 2 cut(s) 129, 283
AleI CACNNNNGTG 1 cut(s) 524
AluBI AGCT 3 cut(s) 152, 441, 447
AluI AGCT 3 cut(s) 152, 441, 447
Alw21I GWGCWC 3 cut(s) 154, 220, 310
Aor13HI TCCGGA 1 cut(s) 5
AoxI GGCC 3 cut(s) 132, 267, 506
ApeKI GCWGC 3 cut(s) 42, 189, 384
ApoI RAATTY 2 cut(s) 515, 605
AspLEI GCGC 5 cut(s) 47, 92, 163, 200, 421
AspS9I GGNCC 2 cut(s) 340, 506
AsuC2I CCSGG 3 cut(s) 57, 266, 333
AsuHPI GGTGA 1 cut(s) 472
AvaII GGWCC 1 cut(s) 340
AxyI CCTNAGG 1 cut(s) 510
BanI GGYRCC 2 cut(s) 197, 313
BanII GRGCYC 1 cut(s) 154
BbsI GAAGAC 1 cut(s) 530
Bbv12I GWGCWC 3 cut(s) 154, 220, 310
BbvI GCAGC 3 cut(s) 54, 201, 396
BceAI ACGGC 6 cut(s) 49, 55, 103, 259, 301, 397
BciT130I CCWGG 2 cut(s) 131, 285
BciVI GTATCC 2 cut(s) 203, 248
BclI TGATCA 1 cut(s) 373
BcnI CCSGG 3 cut(s) 57, 266, 333
BfoI RGCGCY 3 cut(s) 48, 201, 422
BfuI GTATCC 2 cut(s) 203, 248
Bme1390I CCNGG 5 cut(s) 57, 131, 266, 285, 333
Bme18I GGWCC 1 cut(s) 340
BmgT120I GGNCC 2 cut(s) 340, 506
BmiI GGNNCC 6 cut(s) 199, 231, 276, 282, 315, 342
BmrFI CCNGG 5 cut(s) 57, 131, 266, 285, 333
BpiI GAAGAC 1 cut(s) 530
BpmI CTGGAG 1 cut(s) 568
BpuEI CTTGAG 1 cut(s) 469
BpuMI CCSGG 3 cut(s) 57, 266, 333
BsaHI GRCGYC 1 cut(s) 198
BsaJI CCNNGG 2 cut(s) 264, 270
BsaWI WCCGGW 1 cut(s) 5
BsaXI ACNNNNNCTCC 4 cut(s) 59, 89, 119, 149
Bse1I ACTGG 1 cut(s) 551
Bse21I CCTNAGG 1 cut(s) 510
BseAI TCCGGA 1 cut(s) 5
BseBI CCWGG 2 cut(s) 131, 285
BseDI CCNNGG 2 cut(s) 264, 270
BseNI ACTGG 1 cut(s) 551
BseRI GAGGAG 6 cut(s) 87, 90, 127, 165, 168, 222
BseXI GCAGC 3 cut(s) 54, 201, 396
Bsh1236I CGCG 2 cut(s) 161, 227
BshFI GGCC 3 cut(s) 134, 269, 508
BshNI GGYRCC 2 cut(s) 197, 313
BsiHKAI GWGCWC 3 cut(s) 154, 220, 310
BsiSI CCGG 6 cut(s) 6, 57, 207, 266, 278, 333
BsiWI CGTACG 1 cut(s) 222
BsnI GGCC 3 cut(s) 134, 269, 508
Bsp1286I GDGCHC 3 cut(s) 154, 220, 310
Bsp13I TCCGGA 1 cut(s) 5
Bsp143I GATC 3 cut(s) 373, 393, 501
BspANI GGCC 3 cut(s) 134, 269, 508
BspEI TCCGGA 1 cut(s) 5
BspFNI CGCG 2 cut(s) 161, 227
BspLI GGNNCC 6 cut(s) 199, 231, 276, 282, 315, 342
BspT107I GGYRCC 2 cut(s) 197, 313
BsrBI CCGCTC 1 cut(s) 173
BsrI ACTGG 1 cut(s) 551
BssECI CCNNGG 2 cut(s) 264, 270
BssMI GATC 3 cut(s) 373, 393, 501
BssNI GRCGYC 1 cut(s) 198
Bst2UI CCWGG 2 cut(s) 131, 285
Bst4CI ACNGT 3 cut(s) 215, 457, 536
Bst6I CTCTTC 1 cut(s) 482
BstACI GRCGYC 1 cut(s) 198
BstC8I GCNNGC 1 cut(s) 358
BstDEI CTNAG 2 cut(s) 510, 618
BstFNI CGCG 2 cut(s) 161, 227
BstH2I RGCGCY 3 cut(s) 48, 201, 422
BstHHI GCGC 5 cut(s) 47, 92, 163, 200, 421
BstKTI GATC 3 cut(s) 376, 396, 504
BstMBI GATC 3 cut(s) 373, 393, 501
BstMWI GCNNNNNNNGC 7 cut(s) 42, 158, 167, 195, 224, 294, 418
BstNI CCWGG 2 cut(s) 131, 285
BstSCI CCNGG 5 cut(s) 55, 129, 264, 283, 331
BstUI CGCG 2 cut(s) 161, 227
BstV1I GCAGC 3 cut(s) 54, 201, 396
BstV2I GAAGAC 1 cut(s) 530
Bsu36I CCTNAGG 1 cut(s) 510
BsuI GTATCC 2 cut(s) 203, 248
BsuRI GGCC 3 cut(s) 134, 269, 508
BtsI GCAGTG 1 cut(s) 519
BtsIMutI CAGTG 1 cut(s) 519
Cac8I GCNNGC 1 cut(s) 358
CfoI GCGC 5 cut(s) 47, 92, 163, 200, 421
Cfr13I GGNCC 2 cut(s) 340, 506
Csp6I GTAC 4 cut(s) 223, 336, 430, 588
CviAII CATG 1 cut(s) 469
CviQI GTAC 4 cut(s) 223, 336, 430, 588
DdeI CTNAG 2 cut(s) 510, 618
DinI GGCGCC 1 cut(s) 199
DpnI GATC 3 cut(s) 375, 395, 503
DpnII GATC 3 cut(s) 373, 393, 501
Eam1104I CTCTTC 1 cut(s) 482
EarI CTCTTC 1 cut(s) 482
EciI GGCGGA 2 cut(s) 42, 267
Ecl136II GAGCTC 1 cut(s) 152
Eco147I AGGCCT 1 cut(s) 134
Eco24I GRGCYC 1 cut(s) 154
Eco47I GGWCC 1 cut(s) 340
Eco53kI GAGCTC 1 cut(s) 152
Eco57I CTGAAG 2 cut(s) 427, 524
Eco81I CCTNAGG 1 cut(s) 510
EcoICRI GAGCTC 1 cut(s) 152
EcoRI GAATTC 1 cut(s) 515
EcoRII CCWGG 2 cut(s) 129, 283
EcoT38I GRGCYC 1 cut(s) 154
EgeI GGCGCC 1 cut(s) 199
EheI GGCGCC 1 cut(s) 199
FaeI CATG 1 cut(s) 472
FaiI YATR 1 cut(s) 470
FatI CATG 1 cut(s) 468
FauI CCCGC 3 cut(s) 112, 336, 380
FbaI TGATCA 1 cut(s) 373
FriOI GRGCYC 1 cut(s) 154
GlaI GCGC 5 cut(s) 46, 91, 162, 199, 420
GsuI CTGGAG 1 cut(s) 568
HaeII RGCGCY 3 cut(s) 48, 201, 422
HaeIII GGCC 3 cut(s) 134, 269, 508
HapII CCGG 6 cut(s) 6, 57, 207, 266, 278, 333
HhaI GCGC 5 cut(s) 47, 92, 163, 200, 421
Hin1I GRCGYC 1 cut(s) 198
Hin1II CATG 1 cut(s) 472
Hin6I GCGC 5 cut(s) 45, 90, 161, 198, 419
HinP1I GCGC 5 cut(s) 45, 90, 161, 198, 419
HpaII CCGG 6 cut(s) 6, 57, 207, 266, 278, 333
HphI GGTGA 1 cut(s) 472
Hpy188I TCNGA 3 cut(s) 366, 438, 543
Hpy188III TCNNGA 2 cut(s) 6, 377
Hpy99I CGWCG 4 cut(s) 322, 325, 401, 430
HpyAV CCTTC 1 cut(s) 409
HpyCH4III ACNGT 3 cut(s) 215, 457, 536
HpyCH4IV ACGT 1 cut(s) 348
HpyCH4V TGCA 1 cut(s) 412
HpyF10VI GCNNNNNNNGC 7 cut(s) 42, 158, 167, 195, 224, 294, 418
HpyF3I CTNAG 2 cut(s) 510, 618
HpySE526I ACGT 1 cut(s) 348
Hsp92I GRCGYC 1 cut(s) 198
Hsp92II CATG 1 cut(s) 472
HspAI GCGC 5 cut(s) 45, 90, 161, 198, 419
KasI GGCGCC 1 cut(s) 197
Kpn2I TCCGGA 1 cut(s) 5
Ksp22I TGATCA 1 cut(s) 373
Kzo9I GATC 3 cut(s) 373, 393, 501
LmnI GCTCC 7 cut(s) 149, 157, 178, 223, 235, 280, 286
Lsp1109I GCAGC 3 cut(s) 54, 201, 396
MaeII ACGT 1 cut(s) 348
MalI GATC 3 cut(s) 375, 395, 503
MbiI CCGCTC 1 cut(s) 173
MboI GATC 3 cut(s) 373, 393, 501
MboII GAAGA 3 cut(s) 455, 469, 530
MhlI GDGCHC 3 cut(s) 154, 220, 310
MluCI AATT 3 cut(s) 515, 555, 605
Mly113I GGCGCC 1 cut(s) 198
MroI TCCGGA 1 cut(s) 5
MslI CAYNNNNRTG 1 cut(s) 524
MspA1I CMGCKG 2 cut(s) 192, 387
MspI CCGG 6 cut(s) 6, 57, 207, 266, 278, 333
MspR9I CCNGG 5 cut(s) 57, 131, 266, 285, 333
MvaI CCWGG 2 cut(s) 131, 285
MvnI CGCG 2 cut(s) 161, 227
MwoI GCNNNNNNNGC 7 cut(s) 42, 158, 167, 195, 224, 294, 418
NarI GGCGCC 1 cut(s) 198
NciI CCSGG 3 cut(s) 57, 266, 333
NdeII GATC 3 cut(s) 373, 393, 501
NlaIII CATG 1 cut(s) 472
NlaIV GGNNCC 6 cut(s) 199, 231, 276, 282, 315, 342
NmeAIII GCCGAG 1 cut(s) 251
OliI CACNNNNGTG 1 cut(s) 524
PceI AGGCCT 1 cut(s) 134
Pfl23II CGTACG 1 cut(s) 222
PluTI GGCGCC 1 cut(s) 201
Psp124BI GAGCTC 1 cut(s) 154
Psp6I CCWGG 2 cut(s) 129, 283
PspGI CCWGG 2 cut(s) 129, 283
PspLI CGTACG 1 cut(s) 222
PspN4I GGNNCC 6 cut(s) 199, 231, 276, 282, 315, 342
PspPI GGNCC 2 cut(s) 340, 506
RsaI GTAC 4 cut(s) 224, 337, 431, 589
RsaNI GTAC 4 cut(s) 223, 336, 430, 588
RseI CAYNNNNRTG 1 cut(s) 524
SacI GAGCTC 1 cut(s) 154
Sau3AI GATC 3 cut(s) 373, 393, 501
Sau96I GGNCC 2 cut(s) 340, 506
ScrFI CCNGG 5 cut(s) 57, 131, 266, 285, 333
SduI GDGCHC 3 cut(s) 154, 220, 310
SetI ASST 5 cut(s) 97, 154, 351, 443, 449
SfoI GGCGCC 1 cut(s) 199
SinI GGWCC 1 cut(s) 340
SmiMI CAYNNNNRTG 1 cut(s) 524
SmlI CTYRAG 1 cut(s) 448
SmoI CTYRAG 1 cut(s) 448
Sse9I AATT 3 cut(s) 515, 555, 605
SseBI AGGCCT 1 cut(s) 134
SspDI GGCGCC 1 cut(s) 197
SstI GAGCTC 1 cut(s) 154
StuI AGGCCT 1 cut(s) 134
StyD4I CCNGG 5 cut(s) 55, 129, 264, 283, 331
TaaI ACNGT 3 cut(s) 215, 457, 536
TaiI ACGT 1 cut(s) 351
TaqI TCGA 1 cut(s) 396
TasI AATT 3 cut(s) 515, 555, 605
TatI WGTACW 1 cut(s) 587
TauI GCSGC 9 cut(s) 74, 173, 195, 198, 203, 206, 230, 249, 252
TscAI CASTG 1 cut(s) 526
TseI GCWGC 3 cut(s) 42, 189, 384
TspDTI ATGAA 1 cut(s) 449
TspGWI ACGGA 5 cut(s) 162, 198, 210, 306, 353
TspRI CASTG 1 cut(s) 526
VpaK11BI GGWCC 1 cut(s) 340
XapI RAATTY 2 cut(s) 515, 605
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.