AT3G20500

Purple acid phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0005127
Physical Location & Seq
Forward (+)
7157900 .. 7160446
2547 bp
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UTR
Exon/CDS
Intron
AT3G20500.1

Sequence Viewer

Length: 1314 bp
ATGGAAAAATGGGGGATTTTGCTACTCGTAACATTATCAGTGTCAATAATCTTTACTTCTGCGGCCGCAGATGATTACGTCCGACCAAAACCTCGTGAAACCTTGCAGTTTCCATGGAAACAAAAGTCCTCTTCTGTACCCGAGCAGGTGCATATCTCATTGGCTGGAGATAAACATATGCGAGTTACTTGGGTTACGAATGACAAATCGTCTCCTTCATTTGTTGAATATGGAACATCGCCAGGGAAATACTCTTATCTTGGTCAAGGAGAAAGCACCTCCTACAGCTATATAATGTATAGATCAGGGAAGATACATCATACCGTCATTGGGCCATTGGAAGCGGATACTGTTTATTATTACCGTTGTGGTGGAGAAGGACCTGAGTTCCATTTGAAAACACCACCAGCTCAGTTCCCGATTACTTTTGCAGTGGCGGGGGATCTTGGACAAACCGGTTGGACTAAGTCGACTCTAGATCATATTGATCAATGCAAATATGCTGTGCATCTACTTCCAGGGGATCTATCGTATGCGGACTATATGCAGCATAAGTGGGACACGTTTGGGGAACTAGTTCAGCCTCTGGCAAGTGTAAGGCCGTGGATGGTGACACAGGGAAACCATGAGAAAGAGAGTATTCCTTTTATAGTTGATGAATTTGTGTCTTTCAACTCGAGGTGGAAGATGCCGTATGAGGAAAGTGGGTCAAATTCAAATCTTTATTATTCCTTTGAGGTTGCTGGTGTCCATGCGATCATGCTTGGCTCATACACAGATTATGATCGGTACTCAGATCAATACAGCTGGTTAAAGGCTGATCTCTCGAAGGTGGACCGAGAAAGAACTCCGTGGCTAATAGTCCTCTTCCATGTACCATGGTATAACAGTAACAATGCCCATCAACATGAAGGTGATGAAATGATGGCTGAAATGGAGCCTTTGCTTTATGCGAGCGGTGTTGATATTGTATTTACTGGTCATGTCCATGCTTATGAACGCACGAAACGTGTCAACAATGGTAAATCAGATCCATGTGGTCCCGTACACATTACCATAGGTGATGGAGGAAACAGAGAAGGATTGGCTCGCAAGTACAAAGATCCGTCACCAGAGTGGTCAGTCTTCAGGGAAGCAAGCTTTGGACATGGGGAGCTTCAGATGGTGAATTCAACACATGCGCTATGGACCTGGCATAGGAACGATGATGACGAACCAACAAGGTCTGATGAAGTTTGGTTAAACTCTCTTGTGAACTCCGGATGCTTGAAGAAAAGGCCTCAAGAACTCAGGAAAATGCTCTTGGAACCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003993 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0009987 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0042578 GO:0044237
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metallophos Metallophos_C Pur_ac_phosph_N

Protein Analysis

437

Amino Acids

49.87

Weight (kDa)

5.81

Isoelectric Point (pI)

35.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pur_ac_phosph_N PF16656 47 - 134 1.6e-21 Purple acid Phosphatase, N-terminal domain
Metallophos PF00149 142 - 332 2.5e-19 Calcineurin-like phosphoesterase
Metallophos_C PF14008 347 - 404 8.7e-22 Iron/zinc purple acid phosphatase-like protein C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 136
Acc36I ACCTGC 1 cut(s) 136
AccBSI CCGCTC 1 cut(s) 957
AccI GTMKAC 1 cut(s) 470
AccIII TCCGGA 1 cut(s) 1259
AciI CCGC 6 cut(s) 62, 66, 344, 437, 536, 957
AclWI GGATC 4 cut(s) 450, 531, 1025, 1097
AcoI YGGCCR 1 cut(s) 63
AcsI RAATTY 3 cut(s) 659, 712, 1168
AcuI CTGAAG 2 cut(s) 1111, 1142
AfaI GTAC 5 cut(s) 138, 791, 876, 1047, 1097
AfiI CCNNNNNNNGG 2 cut(s) 330, 1197
AflIII ACRYGT 2 cut(s) 561, 1009
AgeI ACCGGT 1 cut(s) 455
AgsI TTSAA 6 cut(s) 227, 397, 673, 717, 1173, 1270
AhdI GACNNNNNGTC 1 cut(s) 208
AhlI ACTAGT 1 cut(s) 574
AjnI CCWGG 3 cut(s) 241, 517, 1190
AjuI GAANNNNNNNTTGG 3 cut(s) 1125, 1157, 1286
AleI CACNNNNGTG 1 cut(s) 1114
AluBI AGCT 5 cut(s) 288, 410, 807, 1140, 1156
AluI AGCT 5 cut(s) 288, 410, 807, 1140, 1156
Alw26I GTCTC 1 cut(s) 216
AlwI GGATC 4 cut(s) 450, 531, 1025, 1097
AlwNI CAGNNNCTG 1 cut(s) 586
Ama87I CYCGRG 2 cut(s) 140, 676
Aor13HI TCCGGA 1 cut(s) 1259
AoxI GGCC 4 cut(s) 63, 332, 599, 1277
ApeKI GCWGC 1 cut(s) 547
ApoI RAATTY 3 cut(s) 659, 712, 1168
AsiGI ACCGGT 1 cut(s) 455
Asp700I GAANNNNTTC 1 cut(s) 576
AspLEI GCGC 1 cut(s) 1183
AspS9I GGNCC 5 cut(s) 332, 380, 835, 1040, 1188
AsuHPI GGTGA 5 cut(s) 622, 926, 1073, 1101, 1177
AvaI CYCGRG 2 cut(s) 140, 676
AvaII GGWCC 4 cut(s) 380, 835, 1040, 1188
BauI CACGAG 1 cut(s) 93
BbsI GAAGAC 1 cut(s) 1117
BbvI GCAGC 1 cut(s) 559
BccI CCATC 5 cut(s) 601, 909, 919, 1058, 1156
BceAI ACGGC 2 cut(s) 586, 676
BciT130I CCWGG 3 cut(s) 243, 519, 1192
BciVI GTATCC 1 cut(s) 340
BclI TGATCA 1 cut(s) 487
BcoDI GTCTC 1 cut(s) 216
BcuI ACTAGT 1 cut(s) 574
BfaI CTAG 2 cut(s) 476, 575
BfmI CTRYAG 1 cut(s) 283
BfuAI ACCTGC 1 cut(s) 136
BfuI GTATCC 1 cut(s) 340
BisI GCNGC 3 cut(s) 63, 66, 548
BlsI GCNGC 3 cut(s) 64, 67, 549
Bme1390I CCNGG 3 cut(s) 243, 519, 1192
Bme18I GGWCC 4 cut(s) 380, 835, 1040, 1188
BmeRI GACNNNNNGTC 1 cut(s) 208
BmeT110I CYCGRG 2 cut(s) 140, 676
BmgT120I GGNCC 5 cut(s) 332, 380, 835, 1040, 1188
BmiI GGNNCC 3 cut(s) 939, 1042, 1308
BmrFI CCNGG 3 cut(s) 243, 519, 1192
BmsI GCATC 3 cut(s) 517, 678, 1253
BpiI GAAGAC 1 cut(s) 1117
BpmI CTGGAG 1 cut(s) 186
BpuEI CTTGAG 1 cut(s) 1266
BsaBI GATNNNNATC 1 cut(s) 783
BsaJI CCNNGG 6 cut(s) 113, 242, 518, 602, 851, 878
BsaWI WCCGGW 2 cut(s) 455, 1259
Bsc4I CCNNNNNNNGG 2 cut(s) 330, 1197
Bse118I RCCGGY 1 cut(s) 455
Bse1I ACTGG 1 cut(s) 982
Bse8I GATNNNNATC 1 cut(s) 783
BseAI TCCGGA 1 cut(s) 1259
BseBI CCWGG 3 cut(s) 243, 519, 1192
BseDI CCNNGG 6 cut(s) 113, 242, 518, 602, 851, 878
BseGI GGATG 2 cut(s) 612, 1268
BseJI GATNNNNATC 1 cut(s) 783
BseLI CCNNNNNNNGG 2 cut(s) 330, 1197
BseMII CTCAG 4 cut(s) 375, 425, 807, 1303
BseNI ACTGG 1 cut(s) 982
BseX3I CGGCCG 1 cut(s) 63
BseXI GCAGC 1 cut(s) 559
Bsh1285I CGRYCG 1 cut(s) 66
BshFI GGCC 4 cut(s) 65, 334, 601, 1279
BshTI ACCGGT 1 cut(s) 455
BsiEI CGRYCG 1 cut(s) 66
BsiHKCI CYCGRG 2 cut(s) 140, 676
BsiSI CCGG 2 cut(s) 456, 1260
BslFI GGGAC 2 cut(s) 572, 1026
BslI CCNNNNNNNGG 2 cut(s) 330, 1197
BsmAI GTCTC 1 cut(s) 216
BsmBI CGTCTC 1 cut(s) 216
BsmFI GGGAC 2 cut(s) 572, 1026
BsnI GGCC 4 cut(s) 65, 334, 601, 1279
BsoBI CYCGRG 2 cut(s) 140, 676
Bsp13I TCCGGA 1 cut(s) 1259
Bsp19I CCATGG 2 cut(s) 113, 878
BspACI CCGC 6 cut(s) 62, 66, 344, 437, 536, 957
BspANI GGCC 4 cut(s) 65, 334, 601, 1279
BspCNI CTCAG 4 cut(s) 376, 424, 806, 1302
BspEI TCCGGA 1 cut(s) 1259
BspLI GGNNCC 3 cut(s) 939, 1042, 1308
BspMI ACCTGC 1 cut(s) 136
BspPI GGATC 4 cut(s) 450, 531, 1025, 1097
BsrBI CCGCTC 1 cut(s) 957
BsrFI RCCGGY 1 cut(s) 455
BsrI ACTGG 1 cut(s) 982
BssAI RCCGGY 1 cut(s) 455
BssECI CCNNGG 6 cut(s) 113, 242, 518, 602, 851, 878
BssSI CACGAG 1 cut(s) 93
BssT1I CCWWGG 2 cut(s) 113, 878
Bst2BI CACGAG 1 cut(s) 93
Bst2UI CCWGG 3 cut(s) 243, 519, 1192
Bst4CI ACNGT 4 cut(s) 325, 352, 365, 890
Bst6I CTCTTC 2 cut(s) 136, 872
BstC8I GCNNGC 3 cut(s) 955, 1090, 1138
BstDEI CTNAG 5 cut(s) 384, 411, 465, 793, 1289
BstDSI CCRYGG 4 cut(s) 113, 602, 851, 878
BstENI CCTNNNNNAGG 1 cut(s) 1195
BstF5I GGATG 2 cut(s) 612, 1268
BstHHI GCGC 1 cut(s) 1183
BstMAI GTCTC 1 cut(s) 216
BstMCI CGRYCG 1 cut(s) 66
BstNI CCWGG 3 cut(s) 243, 519, 1192
BstNSI RCATGY 1 cut(s) 1181
BstSCI CCNGG 3 cut(s) 241, 517, 1190
BstSFI CTRYAG 1 cut(s) 283
BstV1I GCAGC 1 cut(s) 559
BstV2I GAAGAC 1 cut(s) 1117
BstX2I RGATCY 4 cut(s) 442, 523, 1030, 1102
BstYI RGATCY 4 cut(s) 442, 523, 1030, 1102
BstZI CGGCCG 1 cut(s) 63
BsuI GTATCC 1 cut(s) 340
BsuRI GGCC 4 cut(s) 65, 334, 601, 1279
BtgI CCRYGG 4 cut(s) 113, 602, 851, 878
BtgZI GCGATG 1 cut(s) 222
BtsCI GGATG 2 cut(s) 612, 1268
BtsI GCAGTG 1 cut(s) 438
BtsIMutI CAGTG 2 cut(s) 45, 438
BveI ACCTGC 1 cut(s) 136
Cac8I GCNNGC 3 cut(s) 955, 1090, 1138
CaiI CAGNNNCTG 1 cut(s) 586
CciNI GCGGCCGC 1 cut(s) 63
CfoI GCGC 1 cut(s) 1183
Cfr10I RCCGGY 1 cut(s) 455
Cfr13I GGNCC 5 cut(s) 332, 380, 835, 1040, 1188
Csp6I GTAC 5 cut(s) 137, 790, 875, 1046, 1096
CspAI ACCGGT 1 cut(s) 455
CviQI GTAC 5 cut(s) 137, 790, 875, 1046, 1096
DdeI CTNAG 5 cut(s) 384, 411, 465, 793, 1289
DriI GACNNNNNGTC 1 cut(s) 208
EaeI YGGCCR 1 cut(s) 63
EagI CGGCCG 1 cut(s) 63
Eam1104I CTCTTC 2 cut(s) 136, 872
Eam1105I GACNNNNNGTC 1 cut(s) 208
EarI CTCTTC 2 cut(s) 136, 872
EclXI CGGCCG 1 cut(s) 63
Eco130I CCWWGG 2 cut(s) 113, 878
Eco147I AGGCCT 1 cut(s) 1279
Eco47I GGWCC 4 cut(s) 380, 835, 1040, 1188
Eco52I CGGCCG 1 cut(s) 63
Eco57I CTGAAG 2 cut(s) 1111, 1142
Eco88I CYCGRG 2 cut(s) 140, 676
EcoNI CCTNNNNNAGG 1 cut(s) 1195
EcoO109I RGGNCCY 1 cut(s) 380
EcoRI GAATTC 1 cut(s) 1168
EcoRII CCWGG 3 cut(s) 241, 517, 1190
EcoT14I CCWWGG 2 cut(s) 113, 878
ErhI CCWWGG 2 cut(s) 113, 878
Esp3I CGTCTC 1 cut(s) 216
FaqI GGGAC 2 cut(s) 572, 1026
FauI CCCGC 1 cut(s) 430
FauNDI CATATG 1 cut(s) 177
FbaI TGATCA 1 cut(s) 487
FblI GTMKAC 1 cut(s) 470
Fnu4HI GCNGC 3 cut(s) 63, 66, 548
FokI GGATG 2 cut(s) 619, 1275
Fsp4HI GCNGC 3 cut(s) 63, 66, 548
FspBI CTAG 2 cut(s) 476, 575
GlaI GCGC 1 cut(s) 1182
GluI GCNGC 3 cut(s) 63, 66, 548
GsuI CTGGAG 1 cut(s) 186
HaeIII GGCC 4 cut(s) 65, 334, 601, 1279
HapII CCGG 2 cut(s) 456, 1260
HhaI GCGC 1 cut(s) 1183
Hin6I GCGC 1 cut(s) 1181
HinP1I GCGC 1 cut(s) 1181
HincII GTYRAC 2 cut(s) 471, 1015
HindII GTYRAC 2 cut(s) 471, 1015
HindIII AAGCTT 1 cut(s) 1138
HinfI GANTC 1 cut(s) 472
HpaII CCGG 2 cut(s) 456, 1260
HphI GGTGA 5 cut(s) 622, 926, 1073, 1101, 1177
Hpy166II GTNNAC 5 cut(s) 471, 835, 1015, 1048, 1255
Hpy188I TCNGA 5 cut(s) 83, 796, 1030, 1161, 1228
Hpy188III TCNNGA 7 cut(s) 95, 418, 476, 826, 1260, 1283, 1291
Hpy8I GTNNAC 5 cut(s) 471, 835, 1015, 1048, 1255
HpyAV CCTTC 5 cut(s) 225, 371, 823, 905, 1073
HpyCH4III ACNGT 4 cut(s) 325, 352, 365, 890
HpyCH4IV ACGT 3 cut(s) 78, 563, 1009
HpyCH4V TGCA 6 cut(s) 106, 151, 431, 495, 508, 547
HpyF3I CTNAG 5 cut(s) 384, 411, 465, 793, 1289
HpySE526I ACGT 3 cut(s) 78, 563, 1009
HspAI GCGC 1 cut(s) 1181
Kpn2I TCCGGA 1 cut(s) 1259
Ksp22I TGATCA 1 cut(s) 487
LmnI GCTCC 2 cut(s) 937, 1153
Lsp1109I GCAGC 1 cut(s) 559
LweI GCATC 3 cut(s) 517, 678, 1253
MaeI CTAG 2 cut(s) 476, 575
MaeII ACGT 3 cut(s) 78, 563, 1009
MaeIII GTNAC 6 cut(s) 28, 184, 193, 610, 890, 1107
MbiI CCGCTC 1 cut(s) 957
MboII GAAGA 6 cut(s) 123, 322, 697, 859, 1117, 1282
MflI RGATCY 4 cut(s) 442, 523, 1030, 1102
MluCI AATT 3 cut(s) 659, 712, 1168
MlyI GAGTC 1 cut(s) 466
MmeI TCCRAC 2 cut(s) 106, 440
MroI TCCGGA 1 cut(s) 1259
MroXI GAANNNNTTC 1 cut(s) 576
MseI TTAA 3 cut(s) 812, 1241, 1312
MslI CAYNNNNRTG 5 cut(s) 906, 912, 987, 993, 1114
MspA1I CMGCKG 1 cut(s) 807
MspI CCGG 2 cut(s) 456, 1260
MspR9I CCNGG 3 cut(s) 243, 519, 1192
MvaI CCWGG 3 cut(s) 243, 519, 1192
NcoI CCATGG 2 cut(s) 113, 878
NdeI CATATG 1 cut(s) 177
NlaIV GGNNCC 3 cut(s) 939, 1042, 1308
NmuCI GTSAC 2 cut(s) 610, 1107
NotI GCGGCCGC 1 cut(s) 63
NspI RCATGY 1 cut(s) 1181
OliI CACNNNNGTG 1 cut(s) 1114
PaeR7I CTCGAG 1 cut(s) 676
PaqCI CACCTGC 1 cut(s) 136
PceI AGGCCT 1 cut(s) 1279
PcsI WCGNNNNNNNCGW 2 cut(s) 1006, 1209
PdmI GAANNNNTTC 1 cut(s) 576
PflFI GACNNNGTC 1 cut(s) 466
PinAI ACCGGT 1 cut(s) 455
PkrI GCNGC 3 cut(s) 64, 67, 549
PleI GAGTC 1 cut(s) 466
PpsI GAGTC 1 cut(s) 466
PpuMI RGGWCCY 1 cut(s) 380
Psp5II RGGWCCY 1 cut(s) 380
Psp6I CCWGG 3 cut(s) 241, 517, 1190
PspGI CCWGG 3 cut(s) 241, 517, 1190
PspN4I GGNNCC 3 cut(s) 939, 1042, 1308
PspPI GGNCC 5 cut(s) 332, 380, 835, 1040, 1188
PspPPI RGGWCCY 1 cut(s) 380
PspXI VCTCGAGB 1 cut(s) 676
PstNI CAGNNNCTG 1 cut(s) 586
PsuI RGATCY 4 cut(s) 442, 523, 1030, 1102
PsyI GACNNNGTC 1 cut(s) 466
PvuII CAGCTG 1 cut(s) 807
RsaI GTAC 5 cut(s) 138, 791, 876, 1047, 1097
RsaNI GTAC 5 cut(s) 137, 790, 875, 1046, 1096
RseI CAYNNNNRTG 5 cut(s) 906, 912, 987, 993, 1114
SalI GTCGAC 1 cut(s) 469
SaqAI TTAA 3 cut(s) 812, 1241, 1312
SatI GCNGC 3 cut(s) 63, 66, 548
Sau96I GGNCC 5 cut(s) 332, 380, 835, 1040, 1188
SchI GAGTC 1 cut(s) 466
ScrFI CCNGG 3 cut(s) 243, 519, 1192
SfaNI GCATC 3 cut(s) 517, 678, 1253
SfcI CTRYAG 1 cut(s) 283
Sfr274I CTCGAG 1 cut(s) 676
SinI GGWCC 4 cut(s) 380, 835, 1040, 1188
SlaI CTCGAG 1 cut(s) 676
SmiMI CAYNNNNRTG 5 cut(s) 906, 912, 987, 993, 1114
SmlI CTYRAG 2 cut(s) 676, 1281
SmoI CTYRAG 2 cut(s) 676, 1281
SpeI ACTAGT 1 cut(s) 574
Sse9I AATT 3 cut(s) 659, 712, 1168
SseBI AGGCCT 1 cut(s) 1279
SsiI CCGC 6 cut(s) 62, 66, 344, 437, 536, 957
SspMI CTAG 2 cut(s) 476, 575
StuI AGGCCT 1 cut(s) 1279
StyD4I CCNGG 3 cut(s) 241, 517, 1190
StyI CCWWGG 2 cut(s) 113, 878
TaaI ACNGT 4 cut(s) 325, 352, 365, 890
TaiI ACGT 3 cut(s) 81, 566, 1012
TaqI TCGA 3 cut(s) 470, 677, 827
TaqII GACCGA 1 cut(s) 852
TasI AATT 3 cut(s) 659, 712, 1168
TatI WGTACW 1 cut(s) 1095
TauI GCSGC 2 cut(s) 65, 68
Tru1I TTAA 3 cut(s) 812, 1241, 1312
Tru9I TTAA 3 cut(s) 812, 1241, 1312
TscAI CASTG 2 cut(s) 45, 438
TseFI GTSAC 2 cut(s) 610, 1107
TseI GCWGC 1 cut(s) 547
Tsp45I GTSAC 2 cut(s) 610, 1107
TspDTI ATGAA 6 cut(s) 207, 672, 924, 933, 1011, 1245
TspGWI ACGGA 2 cut(s) 840, 1095
TspRI CASTG 2 cut(s) 45, 438
Tth111I GACNNNGTC 1 cut(s) 466
VpaK11BI GGWCC 4 cut(s) 380, 835, 1040, 1188
XagI CCTNNNNNAGG 1 cut(s) 1195
XapI RAATTY 3 cut(s) 659, 712, 1168
XbaI TCTAGA 1 cut(s) 475
XceI RCATGY 1 cut(s) 1181
XhoI CTCGAG 1 cut(s) 676
XmiI GTMKAC 1 cut(s) 470
XmnI GAANNNNTTC 1 cut(s) 576
XspI CTAG 2 cut(s) 476, 575
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.