Rh5BG268800

Purple acid phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0005127
Physical Location & Seq
Reverse (-)
33519793 .. 33521324
1532 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG268800.1

Sequence Viewer

Length: 717 bp
ATGGGGCAAATACAGCTCCGTAGCTCAAGGAGAAAGCACTTCGTATATAAGTCTGGACTGATACACCACACTGTTATTGGTCCTCTTGAACATGATACAGTTTACTTCTACAAATGTGGAGGACAAGGTCCTGAGTTTCAGCTCAAGACCCCTGCAGCTCAGTTTCCAATTACTTTTGCTGTGGCAGGAGATCTTGGTCAAACTGGATGGACAAAGTCAACCCTAGACCACATCGAGCGCTGCAAGTATGTTGTCCATATCCTTCCTGGAGACTTTTCATATGCTGATTATTTGCAGCCTTGGTGGGATACCTTTGGTGAGCTGGTTCAGCCACTCGCAAGTGCAAGGCCATGGATGATAACCCAGGGAAACCATGAGAAGGGGAGGATACCATTCTTGAAGGATGGGTTTGAATCCTATAATTCTAGGTGGAAAATGCCGTATAAAGAGAGAGGATCGAGCTCGAATCTTTATTACTCCTTTGAAGTTGCAGGTGCTCATATTATCATGCTTGGCTCGTATACTGATTATGATGAATATTCAGATCAATACAAGTGGTTGAAGGCTGATCTGTCAAAGTTAGACCGACAAAAGACACCTTGGTTGCTTGTAGTATTTCATGTTCCCTGGTATAATAATAACAAGGCTCATCAAGGTGAAGGGGACAGCATGATGGCAGCAATGGAGCCAATGTTTATGCTGCTTGTTCTGATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003993 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0009987 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0042578 GO:0044237
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metallophos Metallophos_C Pur_ac_phosph_N

Protein Analysis

238

Amino Acids

27.53

Weight (kDa)

7.15

Isoelectric Point (pI)

42.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Metallophos PF00149 58 - 217 5.3e-13 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 482
Acc36I ACCTGC 1 cut(s) 482
AccI GTMKAC 1 cut(s) 521
AclWI GGATC 1 cut(s) 463
AfeI AGCGCT 1 cut(s) 239
AfiI CCNNNNNNNGG 1 cut(s) 379
AgsI TTSAA 5 cut(s) 89, 400, 413, 485, 562
AjnI CCWGG 3 cut(s) 265, 363, 626
AluBI AGCT 6 cut(s) 16, 24, 142, 158, 322, 462
AluI AGCT 6 cut(s) 16, 24, 142, 158, 322, 462
Alw21I GWGCWC 2 cut(s) 464, 499
Alw26I GTCTC 1 cut(s) 264
AlwI GGATC 1 cut(s) 463
Aor51HI AGCGCT 1 cut(s) 239
AoxI GGCC 1 cut(s) 347
ApeKI GCWGC 5 cut(s) 155, 240, 295, 677, 700
AspLEI GCGC 1 cut(s) 240
AspS9I GGNCC 2 cut(s) 80, 128
AsuHPI GGTGA 2 cut(s) 329, 668
AvaII GGWCC 2 cut(s) 80, 128
BanII GRGCYC 1 cut(s) 464
Bbv12I GWGCWC 2 cut(s) 464, 499
BbvI GCAGC 5 cut(s) 167, 227, 307, 687, 689
BccI CCATC 3 cut(s) 201, 398, 667
BceAI ACGGC 1 cut(s) 424
BciT130I CCWGG 3 cut(s) 267, 365, 628
BciVI GTATCC 2 cut(s) 301, 381
BcoDI GTCTC 1 cut(s) 264
BfaI CTAG 2 cut(s) 224, 426
BfmI CTRYAG 1 cut(s) 153
BfoI RGCGCY 1 cut(s) 241
BfuAI ACCTGC 1 cut(s) 482
BfuI GTATCC 2 cut(s) 301, 381
BglII AGATCT 1 cut(s) 190
BisI GCNGC 5 cut(s) 156, 241, 296, 678, 701
BlsI GCNGC 5 cut(s) 157, 242, 297, 679, 702
Bme1390I CCNGG 3 cut(s) 267, 365, 628
Bme18I GGWCC 2 cut(s) 80, 128
BmgT120I GGNCC 2 cut(s) 80, 128
BmiI GGNNCC 1 cut(s) 687
BmrFI CCNGG 3 cut(s) 267, 365, 628
BpmI CTGGAG 1 cut(s) 288
BpuEI CTTGAG 2 cut(s) 10, 128
BsaJI CCNNGG 6 cut(s) 299, 350, 363, 364, 599, 626
BsaXI ACNNNNNCTCC 2 cut(s) 677, 707
Bsc4I CCNNNNNNNGG 1 cut(s) 379
Bse1I ACTGG 1 cut(s) 208
Bse3DI GCAATG 1 cut(s) 687
BseBI CCWGG 3 cut(s) 267, 365, 628
BseDI CCNNGG 6 cut(s) 299, 350, 363, 364, 599, 626
BseGI GGATG 3 cut(s) 212, 360, 409
BseLI CCNNNNNNNGG 1 cut(s) 379
BseMI GCAATG 1 cut(s) 687
BseMII CTCAG 2 cut(s) 123, 173
BseNI ACTGG 1 cut(s) 208
BseXI GCAGC 5 cut(s) 167, 227, 307, 687, 689
BshFI GGCC 1 cut(s) 349
BsiHKAI GWGCWC 2 cut(s) 464, 499
BslFI GGGAC 1 cut(s) 677
BslI CCNNNNNNNGG 1 cut(s) 379
BsmAI GTCTC 1 cut(s) 264
BsmFI GGGAC 1 cut(s) 677
BsnI GGCC 1 cut(s) 349
Bsp1286I GDGCHC 2 cut(s) 464, 499
Bsp143I GATC 4 cut(s) 190, 455, 544, 568
Bsp19I CCATGG 1 cut(s) 350
BspANI GGCC 1 cut(s) 349
BspCNI CTCAG 2 cut(s) 124, 172
BspLI GGNNCC 1 cut(s) 687
BspMAI CTGCAG 1 cut(s) 157
BspMI ACCTGC 1 cut(s) 482
BspPI GGATC 1 cut(s) 463
BsrDI GCAATG 1 cut(s) 687
BsrI ACTGG 1 cut(s) 208
BssECI CCNNGG 6 cut(s) 299, 350, 363, 364, 599, 626
BssMI GATC 4 cut(s) 190, 455, 544, 568
BssNAI GTATAC 1 cut(s) 522
BssT1I CCWWGG 3 cut(s) 299, 350, 599
Bst1107I GTATAC 1 cut(s) 522
Bst2UI CCWGG 3 cut(s) 267, 365, 628
Bst4CI ACNGT 2 cut(s) 73, 100
BstDEI CTNAG 2 cut(s) 132, 159
BstDSI CCRYGG 1 cut(s) 350
BstF5I GGATG 3 cut(s) 212, 360, 409
BstH2I RGCGCY 1 cut(s) 241
BstHHI GCGC 1 cut(s) 240
BstKTI GATC 4 cut(s) 193, 458, 547, 571
BstMAI GTCTC 1 cut(s) 264
BstMBI GATC 4 cut(s) 190, 455, 544, 568
BstMWI GCNNNNNNNGC 2 cut(s) 13, 328
BstNI CCWGG 3 cut(s) 267, 365, 628
BstSCI CCNGG 3 cut(s) 265, 363, 626
BstSFI CTRYAG 1 cut(s) 153
BstV1I GCAGC 5 cut(s) 167, 227, 307, 687, 689
BstX2I RGATCY 1 cut(s) 190
BstYI RGATCY 1 cut(s) 190
BstZ17I GTATAC 1 cut(s) 522
BsuI GTATCC 2 cut(s) 301, 381
BsuRI GGCC 1 cut(s) 349
BtgI CCRYGG 1 cut(s) 350
BtsCI GGATG 3 cut(s) 212, 360, 409
BtsIMutI CAGTG 1 cut(s) 69
BveI ACCTGC 1 cut(s) 482
CfoI GCGC 1 cut(s) 240
Cfr13I GGNCC 2 cut(s) 80, 128
CspCI CAANNNNNGTGG 2 cut(s) 536, 571
CviAII CATG 6 cut(s) 92, 351, 374, 508, 620, 670
DdeI CTNAG 2 cut(s) 132, 159
DpnI GATC 4 cut(s) 192, 457, 546, 570
DpnII GATC 4 cut(s) 190, 455, 544, 568
Ecl136II GAGCTC 1 cut(s) 462
Eco130I CCWWGG 3 cut(s) 299, 350, 599
Eco24I GRGCYC 1 cut(s) 464
Eco47I GGWCC 2 cut(s) 80, 128
Eco47III AGCGCT 1 cut(s) 239
Eco53kI GAGCTC 1 cut(s) 462
EcoICRI GAGCTC 1 cut(s) 462
EcoO109I RGGNCCY 1 cut(s) 128
EcoRII CCWGG 3 cut(s) 265, 363, 626
EcoT14I CCWWGG 3 cut(s) 299, 350, 599
EcoT38I GRGCYC 1 cut(s) 464
ErhI CCWWGG 3 cut(s) 299, 350, 599
FaeI CATG 6 cut(s) 95, 354, 377, 511, 623, 673
FaqI GGGAC 1 cut(s) 677
FatI CATG 6 cut(s) 91, 350, 373, 507, 619, 669
FauNDI CATATG 1 cut(s) 280
FblI GTMKAC 1 cut(s) 521
Fnu4HI GCNGC 5 cut(s) 156, 241, 296, 678, 701
FokI GGATG 3 cut(s) 219, 367, 416
FriOI GRGCYC 1 cut(s) 464
Fsp4HI GCNGC 5 cut(s) 156, 241, 296, 678, 701
FspBI CTAG 2 cut(s) 224, 426
GlaI GCGC 1 cut(s) 239
GluI GCNGC 5 cut(s) 156, 241, 296, 678, 701
GsuI CTGGAG 1 cut(s) 288
HaeII RGCGCY 1 cut(s) 241
HaeIII GGCC 1 cut(s) 349
HhaI GCGC 1 cut(s) 240
Hin1II CATG 6 cut(s) 95, 354, 377, 511, 623, 673
Hin6I GCGC 1 cut(s) 238
HinP1I GCGC 1 cut(s) 238
HincII GTYRAC 1 cut(s) 219
HindII GTYRAC 1 cut(s) 219
HinfI GANTC 2 cut(s) 413, 466
HphI GGTGA 2 cut(s) 329, 668
Hpy166II GTNNAC 3 cut(s) 103, 219, 522
Hpy188I TCNGA 2 cut(s) 544, 711
Hpy188III TCNNGA 5 cut(s) 54, 86, 131, 145, 397
Hpy8I GTNNAC 3 cut(s) 103, 219, 522
HpyAV CCTTC 5 cut(s) 272, 373, 394, 556, 653
HpyCH4III ACNGT 2 cut(s) 73, 100
HpyCH4V TGCA 5 cut(s) 155, 243, 295, 344, 491
HpyF10VI GCNNNNNNNGC 2 cut(s) 13, 328
HpyF3I CTNAG 2 cut(s) 132, 159
Hsp92II CATG 6 cut(s) 95, 354, 377, 511, 623, 673
HspAI GCGC 1 cut(s) 238
Kzo9I GATC 4 cut(s) 190, 455, 544, 568
LmnI GCTCC 2 cut(s) 21, 685
Lsp1109I GCAGC 5 cut(s) 167, 227, 307, 687, 689
MaeI CTAG 2 cut(s) 224, 426
MalI GATC 4 cut(s) 192, 457, 546, 570
MboI GATC 4 cut(s) 190, 455, 544, 568
MflI RGATCY 1 cut(s) 190
MhlI GDGCHC 2 cut(s) 464, 499
MluCI AATT 2 cut(s) 168, 421
MnlI CCTC 4 cut(s) 93, 113, 378, 446
MslI CAYNNNNRTG 1 cut(s) 654
MspR9I CCNGG 3 cut(s) 267, 365, 628
MvaI CCWGG 3 cut(s) 267, 365, 628
MwoI GCNNNNNNNGC 2 cut(s) 13, 328
NcoI CCATGG 1 cut(s) 350
NdeI CATATG 1 cut(s) 280
NdeII GATC 4 cut(s) 190, 455, 544, 568
NlaIII CATG 6 cut(s) 95, 354, 377, 511, 623, 673
NlaIV GGNNCC 1 cut(s) 687
PaqCI CACCTGC 1 cut(s) 482
PasI CCCWGGG 1 cut(s) 364
PfeI GAWTC 2 cut(s) 413, 466
PflFI GACNNNGTC 2 cut(s) 126, 214
PfoI TCCNGGA 1 cut(s) 265
PkrI GCNGC 5 cut(s) 157, 242, 297, 679, 702
PpuMI RGGWCCY 1 cut(s) 128
Psp124BI GAGCTC 1 cut(s) 464
Psp5II RGGWCCY 1 cut(s) 128
Psp6I CCWGG 3 cut(s) 265, 363, 626
PspGI CCWGG 3 cut(s) 265, 363, 626
PspN4I GGNNCC 1 cut(s) 687
PspPI GGNCC 2 cut(s) 80, 128
PspPPI RGGWCCY 1 cut(s) 128
PsrI GAACNNNNNNTAC 2 cut(s) 606, 638
PstI CTGCAG 1 cut(s) 157
PsuI RGATCY 1 cut(s) 190
PsyI GACNNNGTC 2 cut(s) 126, 214
RseI CAYNNNNRTG 1 cut(s) 654
SacI GAGCTC 1 cut(s) 464
SatI GCNGC 5 cut(s) 156, 241, 296, 678, 701
Sau3AI GATC 4 cut(s) 190, 455, 544, 568
Sau96I GGNCC 2 cut(s) 80, 128
ScrFI CCNGG 3 cut(s) 267, 365, 628
SduI GDGCHC 2 cut(s) 464, 499
SfcI CTRYAG 1 cut(s) 153
SinI GGWCC 2 cut(s) 80, 128
SmiMI CAYNNNNRTG 1 cut(s) 654
SmlI CTYRAG 2 cut(s) 25, 143
SmoI CTYRAG 2 cut(s) 25, 143
Sse9I AATT 2 cut(s) 168, 421
SspI AATATT 1 cut(s) 539
SspMI CTAG 2 cut(s) 224, 426
SstI GAGCTC 1 cut(s) 464
StyD4I CCNGG 3 cut(s) 265, 363, 626
StyI CCWWGG 3 cut(s) 299, 350, 599
TaaI ACNGT 2 cut(s) 73, 100
TaqI TCGA 3 cut(s) 234, 458, 464
TaqII GACCGA 1 cut(s) 600
TasI AATT 2 cut(s) 168, 421
TfiI GAWTC 2 cut(s) 413, 466
TscAI CASTG 1 cut(s) 76
TseI GCWGC 5 cut(s) 155, 240, 295, 677, 700
TspDTI ATGAA 3 cut(s) 267, 549, 608
TspGWI ACGGA 1 cut(s) 8
TspRI CASTG 1 cut(s) 76
Tth111I GACNNNGTC 2 cut(s) 126, 214
VpaK11BI GGWCC 2 cut(s) 80, 128
XcmI CCANNNNNNNNNTGG 2 cut(s) 74, 263
XmiI GTMKAC 1 cut(s) 521
XspI CTAG 2 cut(s) 224, 426
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.