Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0005127

Physical Location & Seq

Genomic Coordinates Forward (+)

27852127 .. 27856406

Gene Structure 4280 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1203900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1329 bp

ATGGACCCAAAGCCGATAATCACGGTCCTCTTCTTAATCTCCGCCACCGTCATCGCCGCCCAAGAATACGTCCGACCACCGCCTCGCAAGGCCCTCCATTTCCCATGGAGTCGAAAACCCTCTTCGCTTCCCCAGCAGAAAAAAGATAGAAAGAAGTTCAGTGTGCACATCTCTTTTGCAGGAGACAAGCACATGCGGGTGACGTGGGTCACTGATGATAAATCTGCTCCTTCAGTTGTTGAATATGGAACATCATCTGGGAAATACAGTTCTGTAGCTCAAGGAGAAAGCACCTCCTATACTTATATGCTCTATAAGTCAGGAAAGATACACCATACTGTCATTGGGCCTCTTGAACATGACACCGTTTACTTCTACCAATGTGGAGGCCAAGCTCCTGAGTTTCAGTTCAAGACCCCTCCAGCTCAATTTCCGATTACTTTTGCTGTGGCTGGAGATCTTGGACAAACTGGATGGACGAAGTCAACGCTAGACCACATTGACCAGTGCAAATATGATGTGCATCTGCTTCCTGGAGACCTTTCATATGCTGATTACATTCAGTCTCGGTGGGATACATTTGGTGAGCTGGTGCAGCCACTTGCAAGTGCAAGGCCATGGATGGTAACCCAAGGGAACCATGAAAAGGAAAATATACCATTGTTAAAGGATGGGTTTCAATCCTATAATTCCAGGTGGAAAATGCCGTATGAAGAAAGTGGATCAAGTTCAAATCTTTACTATTCCTTTGAAGTTGCAGGTGTCCATGCTATCATGCTCGGTTCATATACTGATAATGATGAGTATTCAGATCAATACAGGTGGCTGAAGGCTGATCTTTCAAAGGTTGACCGACAGAAGACACCTTGGTTGCTCGTGCTCTTTCATGTTCCCTGGTACAATAGTAACAAGGCTCATCAAGGTGAAGGGAACAGCATGATGGCAGCAATGGAGCCATTGCTTTATGCTGCCAGTGCGGATATAGTATTTGCTGGTCATGTTCATGCTTATGAACGAACGAAACGCGTGAACAATGGAAAATCAGATAGATGCGGTGCTGTCCACATAACCATTGGTGATGGCGGAAACAAAGAAGGCTTGGCTCACAAGTATAAAAACCCACCACCCGAGTGGTCGGTTTTCCGGGAAGCAAGTTTTGGTCATGGTGAGCTGAATATAGTGAATTCAACCCATGCCTTTTGGACCTGGCATAGGAATGATGACGAGGAGCCTGTTAGGTCCGATCAGGTCTGGATAACCTCGTTGGTTAGTTCAGGATGTGTTGCTGAGAAGACACTTGAACTAACGAAGATACTTACGGAACCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003993 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0009987 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0042578 GO:0044237

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Metallophos Metallophos_C Pur_ac_phosph_N

Protein Analysis

443

Amino Acids

49.92

Weight (kDa)

6.55

Isoelectric Point (pI)

38.07

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pur_ac_phosph_N	PF16656	54 - 139	7.6e-21	Purple acid Phosphatase, N-terminal domain
Metallophos	PF00149	147 - 337	1.5e-18	Calcineurin-like phosphoesterase
Metallophos_C	PF14008	353 - 409	3.8e-21	Iron/zinc purple acid phosphatase-like protein C

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005127)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G20500
fragaria_vesca	FvH4_3g22510 FvH4_3g22510 FvH4_3g22510 FvH4_3g22510
malus_domestica	MD03G1203900.v1.1 MD11G1220800.v1.1
prunus_persica	Prupe.4G211500_v2.0.a1 Prupe.4G211500_v2.0.a1
pyrus_communis	pycom03g15460 pycom11g19470
rosa_chinensis	RchiOBHm_Chr3g0493801 RchiOBHm_Chr5g0039161
rosa_laevigata	RLG00000033899
rosa_multiflora	Rmu_sc0000533.1_g000006 Rmu_sc0002235.1_g000065
rosa_roxburghii	Rroxscaffold_1G00040430 Rroxscaffold_1G00041930 Rroxscaffold_1G00041940
rosa_rugosa	Rorug05G0177100
rosa_samantha	Rh5AG263600 Rh5AG263900 Rh5BG268500 Rh5BG268800 Rh5CG301400 Rh5CG301800 Rh5DG276300 Rh5DG276500
rosa_wichuraiana	Rw5G024650 Rw5G024680

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	749
Acc36I	ACCTGC	1 cut(s)	749
AccII	CGCG	1 cut(s)	1026
AciI	CCGC	7 cut(s)	42, 57, 80, 196, 977, 1053, 1083
AclWI	GGATC	1 cut(s)	730
AcsI	RAATTY	1 cut(s)	1183
AcuI	CTGAAG	2 cut(s)	216, 848
AfaI	GTAC	1 cut(s)	899
AfiI	CCNNNNNNNGG	2 cut(s)	646, 1212
AflIII	ACRYGT	1 cut(s)	1024
AgsI	TTSAA	9 cut(s)	242, 356, 412, 680, 732, 752, 843, 1188, 1301
AjiI	CACGTC	1 cut(s)	204
AjnI	CCWGG	4 cut(s)	532, 692, 893, 1205
AjuI	GAANNNNNNNTTGG	2 cut(s)	1140, 1172
AleI	CACNNNNGTG	1 cut(s)	1129
AloI	GAACNNNNNNTCC	2 cut(s)	253, 285
AluBI	AGCT	5 cut(s)	278, 395, 425, 589, 1171
AluI	AGCT	5 cut(s)	278, 395, 425, 589, 1171
Alw21I	GWGCWC	2 cut(s)	168, 882
Alw26I	GTCTC	3 cut(s)	177, 531, 570
Alw44I	GTGCAC	1 cut(s)	164
AlwI	GGATC	1 cut(s)	730
Ama87I	CYCGRG	1 cut(s)	1127
AoxI	GGCC	4 cut(s)	90, 347, 388, 614
ApaLI	GTGCAC	1 cut(s)	164
ApeKI	GCWGC	3 cut(s)	595, 944, 968
ApoI	RAATTY	1 cut(s)	1183
AspS9I	GGNCC	6 cut(s)	4, 25, 91, 347, 1203, 1239
AsuC2I	CCSGG	1 cut(s)	1145
AsuHPI	GGTGA	5 cut(s)	211, 596, 935, 1088, 1178
AvaI	CYCGRG	1 cut(s)	1127
AvaII	GGWCC	4 cut(s)	4, 25, 1203, 1239
BaeGI	GKGCMC	1 cut(s)	168
BaeI	ACNNNNGTAYC	2 cut(s)	889, 922
BauI	CACGAG	1 cut(s)	875
BbsI	GAAGAC	2 cut(s)	866, 1298
Bbv12I	GWGCWC	2 cut(s)	168, 882
BbvI	GCAGC	3 cut(s)	607, 955, 956
BccI	CCATC	5 cut(s)	468, 616, 665, 934, 1073
BceAI	ACGGC	1 cut(s)	691
BciT130I	CCWGG	4 cut(s)	534, 694, 895, 1207
BciVI	GTATCC	1 cut(s)	568
BcnI	CCSGG	1 cut(s)	1145
BcoDI	GTCTC	3 cut(s)	177, 531, 570
BfaI	CTAG	1 cut(s)	491
BfmI	CTRYAG	1 cut(s)	273
BfuAI	ACCTGC	1 cut(s)	749
BfuI	GTATCC	1 cut(s)	568
BglII	AGATCT	1 cut(s)	457
BisI	GCNGC	4 cut(s)	57, 596, 945, 969
BlsI	GCNGC	4 cut(s)	58, 597, 946, 970
Bme1390I	CCNGG	5 cut(s)	534, 694, 895, 1145, 1207
Bme18I	GGWCC	4 cut(s)	4, 25, 1203, 1239
BmeT110I	CYCGRG	1 cut(s)	1127
BmgBI	CACGTC	1 cut(s)	204
BmgT120I	GGNCC	6 cut(s)	4, 25, 91, 347, 1203, 1239
BmiI	GGNNCC	5 cut(s)	6, 638, 954, 1230, 1323
BmrFI	CCNGG	5 cut(s)	534, 694, 895, 1145, 1207
BmsI	GCATC	2 cut(s)	532, 1040
BoxI	GACNNNNGTC	1 cut(s)	206
BpiI	GAAGAC	2 cut(s)	866, 1298
BpmI	CTGGAG	3 cut(s)	405, 474, 555
BpuEI	CTTGAG	1 cut(s)	264
BpuMI	CCSGG	1 cut(s)	1145
BsaBI	GATNNNNATC	1 cut(s)	522
BsaI	GGTCTC	1 cut(s)	531
BsaJI	CCNNGG	5 cut(s)	104, 617, 631, 866, 893
Bsc4I	CCNNNNNNNGG	2 cut(s)	646, 1212
Bse1I	ACTGG	3 cut(s)	475, 505, 972
Bse3DI	GCAATG	2 cut(s)	954, 956
Bse8I	GATNNNNATC	1 cut(s)	522
BseBI	CCWGG	4 cut(s)	534, 694, 895, 1207
BseDI	CCNNGG	5 cut(s)	104, 617, 631, 866, 893
BseGI	GGATG	4 cut(s)	479, 627, 676, 1283
BseJI	GATNNNNATC	1 cut(s)	522
BseLI	CCNNNNNNNGG	2 cut(s)	646, 1212
BseMI	GCAATG	2 cut(s)	954, 956
BseMII	CTCAG	2 cut(s)	390, 1278
BseNI	ACTGG	3 cut(s)	475, 505, 972
BseRI	GAGGAG	1 cut(s)	1241
BseSI	GKGCMC	1 cut(s)	168
BseXI	GCAGC	3 cut(s)	607, 955, 956
BseYI	CCCAGC	1 cut(s)	132
BsgI	GTGCAG	1 cut(s)	614
Bsh1236I	CGCG	1 cut(s)	1026
BshFI	GGCC	4 cut(s)	92, 349, 390, 616
BsiHKAI	GWGCWC	2 cut(s)	168, 882
BsiHKCI	CYCGRG	1 cut(s)	1127
BsiSI	CCGG	1 cut(s)	1144
BslI	CCNNNNNNNGG	2 cut(s)	646, 1212
BsmAI	GTCTC	3 cut(s)	177, 531, 570
BsnI	GGCC	4 cut(s)	92, 349, 390, 616
Bso31I	GGTCTC	1 cut(s)	531
BsoBI	CYCGRG	1 cut(s)	1127
Bsp1286I	GDGCHC	2 cut(s)	168, 882
Bsp143I	GATC	5 cut(s)	457, 722, 811, 835, 1243
Bsp19I	CCATGG	2 cut(s)	104, 617
BspACI	CCGC	7 cut(s)	42, 57, 80, 196, 977, 1053, 1083
BspANI	GGCC	4 cut(s)	92, 349, 390, 616
BspCNI	CTCAG	2 cut(s)	391, 1279
BspFNI	CGCG	1 cut(s)	1026
BspLI	GGNNCC	5 cut(s)	6, 638, 954, 1230, 1323
BspMI	ACCTGC	1 cut(s)	749
BspPI	GGATC	1 cut(s)	730
BspTNI	GGTCTC	1 cut(s)	531
BsrDI	GCAATG	2 cut(s)	954, 956
BsrI	ACTGG	3 cut(s)	475, 505, 972
BssECI	CCNNGG	5 cut(s)	104, 617, 631, 866, 893
BssMI	GATC	5 cut(s)	457, 722, 811, 835, 1243
BssSI	CACGAG	1 cut(s)	875
BssT1I	CCWWGG	4 cut(s)	104, 617, 631, 866
Bst2BI	CACGAG	1 cut(s)	875
Bst2UI	CCWGG	4 cut(s)	534, 694, 895, 1207
Bst4CI	ACNGT	5 cut(s)	25, 49, 269, 340, 367
Bst6I	CTCTTC	2 cut(s)	35, 127
BstDEI	CTNAG	2 cut(s)	399, 1287
BstDSI	CCRYGG	2 cut(s)	104, 617
BstEII	GGTNACC	1 cut(s)	625
BstENI	CCTNNNNNAGG	1 cut(s)	1210
BstF5I	GGATG	4 cut(s)	479, 627, 676, 1283
BstFNI	CGCG	1 cut(s)	1026
BstKTI	GATC	5 cut(s)	460, 725, 814, 838, 1246
BstMAI	GTCTC	3 cut(s)	177, 531, 570
BstMBI	GATC	5 cut(s)	457, 722, 811, 835, 1243
BstMWI	GCNNNNNNNGC	3 cut(s)	133, 595, 974
BstNI	CCWGG	4 cut(s)	534, 694, 895, 1207
BstNSI	RCATGY	1 cut(s)	196
BstPAI	GACNNNNGTC	1 cut(s)	206
BstPI	GGTNACC	1 cut(s)	625
BstSCI	CCNGG	5 cut(s)	532, 692, 893, 1143, 1205
BstSFI	CTRYAG	1 cut(s)	273
BstSLI	GKGCMC	1 cut(s)	168
BstUI	CGCG	1 cut(s)	1026
BstV1I	GCAGC	3 cut(s)	607, 955, 956
BstV2I	GAAGAC	2 cut(s)	866, 1298
BstX2I	RGATCY	1 cut(s)	457
BstXI	CCANNNNNNTGG	1 cut(s)	1131
BstYI	RGATCY	1 cut(s)	457
BsuI	GTATCC	1 cut(s)	568
BsuRI	GGCC	4 cut(s)	92, 349, 390, 616
BtgI	CCRYGG	2 cut(s)	104, 617
BtgZI	GCGATG	1 cut(s)	37
BtrI	CACGTC	1 cut(s)	204
BtsCI	GGATG	4 cut(s)	479, 627, 676, 1283
BtsIMutI	CAGTG	4 cut(s)	166, 210, 512, 979
BveI	ACCTGC	1 cut(s)	749
Cfr13I	GGNCC	6 cut(s)	4, 25, 91, 347, 1203, 1239
Csp6I	GTAC	1 cut(s)	898
CspCI	CAANNNNNGTGG	2 cut(s)	803, 838
CviQI	GTAC	1 cut(s)	898
DdeI	CTNAG	2 cut(s)	399, 1287
DpnI	GATC	5 cut(s)	459, 724, 813, 837, 1245
DpnII	GATC	5 cut(s)	457, 722, 811, 835, 1243
Eam1104I	CTCTTC	2 cut(s)	35, 127
EarI	CTCTTC	2 cut(s)	35, 127
EciI	GGCGGA	2 cut(s)	31, 1098
Eco130I	CCWWGG	4 cut(s)	104, 617, 631, 866
Eco31I	GGTCTC	1 cut(s)	531
Eco47I	GGWCC	4 cut(s)	4, 25, 1203, 1239
Eco57I	CTGAAG	2 cut(s)	216, 848
Eco88I	CYCGRG	1 cut(s)	1127
Eco91I	GGTNACC	1 cut(s)	625
EcoNI	CCTNNNNNAGG	1 cut(s)	1210
EcoO109I	RGGNCCY	1 cut(s)	91
EcoO65I	GGTNACC	1 cut(s)	625
EcoRI	GAATTC	1 cut(s)	1183
EcoRII	CCWGG	4 cut(s)	532, 692, 893, 1205
EcoT14I	CCWWGG	4 cut(s)	104, 617, 631, 866
ErhI	CCWWGG	4 cut(s)	104, 617, 631, 866
FauI	CCCGC	1 cut(s)	189
FauNDI	CATATG	1 cut(s)	547
Fnu4HI	GCNGC	4 cut(s)	57, 596, 945, 969
FokI	GGATG	4 cut(s)	486, 634, 683, 1290
Fsp4HI	GCNGC	4 cut(s)	57, 596, 945, 969
FspBI	CTAG	1 cut(s)	491
GluI	GCNGC	4 cut(s)	57, 596, 945, 969
GsaI	CCCAGC	1 cut(s)	136
GsuI	CTGGAG	3 cut(s)	405, 474, 555
HaeIII	GGCC	4 cut(s)	92, 349, 390, 616
HapII	CCGG	1 cut(s)	1144
HincII	GTYRAC	2 cut(s)	486, 850
HindII	GTYRAC	2 cut(s)	486, 850
HinfI	GANTC	1 cut(s)	109
HpaII	CCGG	1 cut(s)	1144
HphI	GGTGA	5 cut(s)	211, 596, 935, 1088, 1178
Hpy166II	GTNNAC	6 cut(s)	166, 370, 486, 850, 1030, 1063
Hpy188I	TCNGA	5 cut(s)	74, 435, 811, 1045, 1243
Hpy188III	TCNNGA	6 cut(s)	321, 353, 398, 412, 1252, 1275
Hpy8I	GTNNAC	6 cut(s)	166, 370, 486, 850, 1030, 1063
HpyAV	CCTTC	4 cut(s)	240, 823, 920, 1088
HpyCH4III	ACNGT	5 cut(s)	25, 49, 269, 340, 367
HpyCH4IV	ACGT	2 cut(s)	69, 203
HpyCH4V	TGCA	8 cut(s)	166, 179, 510, 523, 595, 605, 611, 758
HpyF10VI	GCNNNNNNNGC	3 cut(s)	133, 595, 974
HpyF3I	CTNAG	2 cut(s)	399, 1287
HpySE526I	ACGT	2 cut(s)	69, 203
Kzo9I	GATC	5 cut(s)	457, 722, 811, 835, 1243
LmnI	GCTCC	4 cut(s)	232, 400, 952, 1228
Lsp1109I	GCAGC	3 cut(s)	607, 955, 956
LweI	GCATC	2 cut(s)	532, 1040
MaeI	CTAG	1 cut(s)	491
MaeII	ACGT	2 cut(s)	69, 203
MaeIII	GTNAC	4 cut(s)	199, 208, 625, 905
MalI	GATC	5 cut(s)	459, 724, 813, 837, 1245
MboI	GATC	5 cut(s)	457, 722, 811, 835, 1243
MboII	GAAGA	6 cut(s)	22, 114, 725, 871, 1303, 1321
MflI	RGATCY	1 cut(s)	457
MhlI	GDGCHC	2 cut(s)	168, 882
MluCI	AATT	3 cut(s)	428, 688, 1183
MluI	ACGCGT	1 cut(s)	1024
MlyI	GAGTC	1 cut(s)	118
MmeI	TCCRAC	1 cut(s)	97
MseI	TTAA	2 cut(s)	35, 665
MslI	CAYNNNNRTG	6 cut(s)	197, 921, 1002, 1008, 1129, 1215
MspI	CCGG	1 cut(s)	1144
MspR9I	CCNGG	5 cut(s)	534, 694, 895, 1145, 1207
MvaI	CCWGG	4 cut(s)	534, 694, 895, 1207
MvnI	CGCG	1 cut(s)	1026
MwoI	GCNNNNNNNGC	3 cut(s)	133, 595, 974
NciI	CCSGG	1 cut(s)	1145
NcoI	CCATGG	2 cut(s)	104, 617
NdeI	CATATG	1 cut(s)	547
NdeII	GATC	5 cut(s)	457, 722, 811, 835, 1243
NlaIV	GGNNCC	5 cut(s)	6, 638, 954, 1230, 1323
NmuCI	GTSAC	2 cut(s)	199, 208
NspI	RCATGY	1 cut(s)	196
OliI	CACNNNNGTG	1 cut(s)	1129
PaqCI	CACCTGC	1 cut(s)	749
PflFI	GACNNNGTC	1 cut(s)	481
PfoI	TCCNGGA	2 cut(s)	532, 1143
PkrI	GCNGC	4 cut(s)	58, 597, 946, 970
PleI	GAGTC	1 cut(s)	117
PpsI	GAGTC	1 cut(s)	117
PshAI	GACNNNNGTC	1 cut(s)	206
Psp6I	CCWGG	4 cut(s)	532, 692, 893, 1205
PspEI	GGTNACC	1 cut(s)	625
PspFI	CCCAGC	1 cut(s)	132
PspGI	CCWGG	4 cut(s)	532, 692, 893, 1205
PspN4I	GGNNCC	5 cut(s)	6, 638, 954, 1230, 1323
PspPI	GGNCC	6 cut(s)	4, 25, 91, 347, 1203, 1239
PsuI	RGATCY	1 cut(s)	457
PsyI	GACNNNGTC	1 cut(s)	481
RsaI	GTAC	1 cut(s)	899
RsaNI	GTAC	1 cut(s)	898
RseI	CAYNNNNRTG	6 cut(s)	197, 921, 1002, 1008, 1129, 1215
SaqAI	TTAA	2 cut(s)	35, 665
SatI	GCNGC	4 cut(s)	57, 596, 945, 969
Sau3AI	GATC	5 cut(s)	457, 722, 811, 835, 1243
Sau96I	GGNCC	6 cut(s)	4, 25, 91, 347, 1203, 1239
SchI	GAGTC	1 cut(s)	118
ScrFI	CCNGG	5 cut(s)	534, 694, 895, 1145, 1207
SduI	GDGCHC	2 cut(s)	168, 882
SfaNI	GCATC	2 cut(s)	532, 1040
SfcI	CTRYAG	1 cut(s)	273
SinI	GGWCC	4 cut(s)	4, 25, 1203, 1239
SmiMI	CAYNNNNRTG	6 cut(s)	197, 921, 1002, 1008, 1129, 1215
SmlI	CTYRAG	1 cut(s)	279
SmoI	CTYRAG	1 cut(s)	279
Sse9I	AATT	3 cut(s)	428, 688, 1183
SsiI	CCGC	7 cut(s)	42, 57, 80, 196, 977, 1053, 1083
SspMI	CTAG	1 cut(s)	491
StyD4I	CCNGG	5 cut(s)	532, 692, 893, 1143, 1205
StyI	CCWWGG	4 cut(s)	104, 617, 631, 866
TaaI	ACNGT	5 cut(s)	25, 49, 269, 340, 367
TaiI	ACGT	2 cut(s)	72, 206
TaqI	TCGA	1 cut(s)	112
TaqII	GACCGA	1 cut(s)	867
TasI	AATT	3 cut(s)	428, 688, 1183
TauI	GCSGC	1 cut(s)	59
Tru1I	TTAA	2 cut(s)	35, 665
Tru9I	TTAA	2 cut(s)	35, 665
TscAI	CASTG	4 cut(s)	166, 217, 512, 979
TseFI	GTSAC	2 cut(s)	199, 208
TseI	GCWGC	3 cut(s)	595, 944, 968
Tsp45I	GTSAC	2 cut(s)	199, 208
TspDTI	ATGAA	7 cut(s)	534, 657, 726, 774, 875, 992, 1026
TspRI	CASTG	4 cut(s)	166, 217, 512, 979
Tth111I	GACNNNGTC	1 cut(s)	481
VneI	GTGCAC	1 cut(s)	164
VpaK11BI	GGWCC	4 cut(s)	4, 25, 1203, 1239
XagI	CCTNNNNNAGG	1 cut(s)	1210
XapI	RAATTY	1 cut(s)	1183
XceI	RCATGY	1 cut(s)	196
XcmI	CCANNNNNNNNNTGG	3 cut(s)	341, 1070, 1128
XspI	CTAG	1 cut(s)	491

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.