Rmu_sc0000533.1_g000006

Purple acid phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000533.1
OG0005127
Physical Location & Seq
Forward (+)
25021 .. 28473
3453 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000533.1_g000006.1.cds

Sequence Viewer

Length: 1299 bp
atggaatcgaagctctttttgttgttaatcacggtgggcttcacagtcatcgcaggcgagtatgtccggcctcagcctcgcaaagacctccaattcccatgggacgcaaaacccacttcccaaccccaacaggtgcacatctctttggcaggagacaagcacatgagagtgacatgggtcactggtgatagatctgctccttcaattgttgaatatggaacatcatcggggaaatacagctccgtagctcaaggagaaagcacttcatatagttatatgatctataagtctggactgatacaccacactgtcattggtcctcttgaacatgatacagtttacttctacaaatgtggaggacaaggtcctgagtttcagctcaagacccatgcagctcagtttccaattacttttgctgtggctggagatcttggtcaaactggatggacaaagtcaaccctagaccacatcgagcgctgcaagtatgatgttcatatccttcctggagacctttcatatgctgattatttgcagcctcggtgggatacctttggtgagctggttcagccactcgcaagtgcaaggccatggatggtaacccagggaaaccatgagaaggagagcataccattcttgaaggatgggtttgaatcctataattctaggtggaaaatgccgtataaagagagtggatcgagctcgaatctttattactcctttgaagttgcaggtgctcatattatcatgcttggctcgtatactgattatgatgaatattcagatcaatacaagtggttgaaggctgatctgtcaaaggtagaccgacaaaagacaccttggttgcttgtggtatttcatgttccctggtataatagtaacaaggctcatcaaggtgaaggggacagcatgatggcagcaatggagccattgctttatgctgctagtgctgatatagtgtttgctggtcatgttcacgcttatgaacgctcgaaccgtgttaacagtggaaaatcaaatacatgtggtgcagtccacattaccattggtgatggaggaaatagagaaggcttagctcaaaggtttgagcacccctcacctgagtggtcagttttccgggaagcaagttttggtcacggtgagctagagatagtcaattcaacccatgccttttggacctggcataggaatgatgatgatgagcctgtaaggtctgatcaggtatggatgacctccttggtcagttcgggatgtgttgctgaaaagagacatgagctgaggaagatactcatatcaccatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003993 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0009987 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0042578 GO:0044237
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metallophos Metallophos_C Pur_ac_phosph_N

Protein Analysis

432

Amino Acids

48.78

Weight (kDa)

6.05

Isoelectric Point (pI)

41.48

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 719
Acc36I ACCTGC 1 cut(s) 719
AccI GTMKAC 2 cut(s) 758, 819
AclWI GGATC 1 cut(s) 700
AfeI AGCGCT 1 cut(s) 476
AfiI CCNNNNNNNGG 2 cut(s) 616, 1182
AflIII ACRYGT 1 cut(s) 1019
AgsI TTSAA 8 cut(s) 204, 212, 326, 637, 650, 722, 799, 1158
AjnI CCWGG 4 cut(s) 502, 600, 863, 1175
AjuI GAANNNNNNNTTGG 2 cut(s) 1110, 1142
AleI CACNNNNGTG 1 cut(s) 1099
Alw21I GWGCWC 4 cut(s) 138, 701, 736, 1089
Alw26I GTCTC 3 cut(s) 147, 501, 1258
Alw44I GTGCAC 1 cut(s) 134
AlwI GGATC 1 cut(s) 700
Aor51HI AGCGCT 1 cut(s) 476
AoxI GGCC 2 cut(s) 68, 584
ApaLI GTGCAC 1 cut(s) 134
ApeKI GCWGC 5 cut(s) 392, 477, 532, 914, 938
AspLEI GCGC 1 cut(s) 477
AspS9I GGNCC 3 cut(s) 317, 365, 1173
AsuC2I CCSGG 1 cut(s) 1115
AsuHPI GGTGA 7 cut(s) 197, 566, 905, 1058, 1086, 1148, 1284
AvaII GGWCC 3 cut(s) 317, 365, 1173
BaeGI GKGCMC 1 cut(s) 138
BanII GRGCYC 1 cut(s) 701
Bbv12I GWGCWC 4 cut(s) 138, 701, 736, 1089
BbvCI CCTCAGC 2 cut(s) 72, 1274
BbvI GCAGC 5 cut(s) 404, 464, 544, 925, 926
BccI CCATC 5 cut(s) 438, 586, 635, 904, 1043
BceAI ACGGC 1 cut(s) 661
BciT130I CCWGG 4 cut(s) 504, 602, 865, 1177
BciVI GTATCC 1 cut(s) 538
BclI TGATCA 1 cut(s) 1213
BcnI CCSGG 1 cut(s) 1115
BcoDI GTCTC 3 cut(s) 147, 501, 1258
BfaI CTAG 4 cut(s) 461, 663, 942, 1142
BfoI RGCGCY 1 cut(s) 478
BfuAI ACCTGC 1 cut(s) 719
BfuI GTATCC 1 cut(s) 538
BglII AGATCT 2 cut(s) 191, 427
BisI GCNGC 5 cut(s) 393, 478, 533, 915, 939
BlpI GCTNAGC 1 cut(s) 1069
BlsI GCNGC 5 cut(s) 394, 479, 534, 916, 940
Bme1390I CCNGG 5 cut(s) 504, 602, 865, 1115, 1177
Bme18I GGWCC 3 cut(s) 317, 365, 1173
BmgT120I GGNCC 3 cut(s) 317, 365, 1173
BmiI GGNNCC 1 cut(s) 924
BmrFI CCNGG 5 cut(s) 504, 602, 865, 1115, 1177
BoxI GACNNNNGTC 1 cut(s) 176
BplI GAGNNNNNCTC 2 cut(s) 1076, 1108
BpmI CTGGAG 2 cut(s) 444, 525
Bpu10I CCTNAGC 2 cut(s) 72, 1274
Bpu1102I GCTNAGC 1 cut(s) 1069
BpuEI CTTGAG 2 cut(s) 234, 365
BpuMI CCSGG 1 cut(s) 1115
BsaI GGTCTC 1 cut(s) 501
BsaJI CCNNGG 8 cut(s) 98, 536, 587, 600, 601, 836, 863, 1233
Bsc4I CCNNNNNNNGG 2 cut(s) 616, 1182
Bse1I ACTGG 2 cut(s) 187, 445
Bse3DI GCAATG 2 cut(s) 924, 926
BseBI CCWGG 4 cut(s) 504, 602, 865, 1177
BseDI CCNNGG 8 cut(s) 98, 536, 587, 600, 601, 836, 863, 1233
BseGI GGATG 5 cut(s) 449, 597, 646, 1230, 1253
BseLI CCNNNNNNNGG 2 cut(s) 616, 1182
BseMI GCAATG 2 cut(s) 924, 926
BseMII CTCAG 5 cut(s) 86, 360, 410, 1089, 1265
BseNI ACTGG 2 cut(s) 187, 445
BseSI GKGCMC 1 cut(s) 138
BseXI GCAGC 5 cut(s) 404, 464, 544, 925, 926
BsgI GTGCAG 1 cut(s) 1047
BshFI GGCC 2 cut(s) 70, 586
BsiHKAI GWGCWC 4 cut(s) 138, 701, 736, 1089
BsiSI CCGG 2 cut(s) 67, 1114
BslFI GGGAC 2 cut(s) 116, 914
BslI CCNNNNNNNGG 2 cut(s) 616, 1182
BsmAI GTCTC 3 cut(s) 147, 501, 1258
BsmFI GGGAC 2 cut(s) 116, 914
BsnI GGCC 2 cut(s) 70, 586
Bso31I GGTCTC 1 cut(s) 501
Bsp1286I GDGCHC 4 cut(s) 138, 701, 736, 1089
Bsp143I GATC 7 cut(s) 191, 279, 427, 692, 781, 805, 1213
Bsp1720I GCTNAGC 1 cut(s) 1069
Bsp19I CCATGG 2 cut(s) 98, 587
BspANI GGCC 2 cut(s) 70, 586
BspCNI CTCAG 5 cut(s) 85, 361, 409, 1090, 1266
BspLI GGNNCC 1 cut(s) 924
BspMI ACCTGC 1 cut(s) 719
BspPI GGATC 1 cut(s) 700
BspTNI GGTCTC 1 cut(s) 501
BsrDI GCAATG 2 cut(s) 924, 926
BsrI ACTGG 2 cut(s) 187, 445
BssECI CCNNGG 8 cut(s) 98, 536, 587, 600, 601, 836, 863, 1233
BssMI GATC 7 cut(s) 191, 279, 427, 692, 781, 805, 1213
BssNAI GTATAC 1 cut(s) 759
BssT1I CCWWGG 4 cut(s) 98, 587, 836, 1233
Bst1107I GTATAC 1 cut(s) 759
Bst2UI CCWGG 4 cut(s) 504, 602, 865, 1177
Bst4CI ACNGT 7 cut(s) 34, 46, 310, 337, 995, 1004, 1136
BstC8I GCNNGC 1 cut(s) 55
BstDEI CTNAG 6 cut(s) 72, 369, 396, 1069, 1098, 1274
BstDSI CCRYGG 2 cut(s) 98, 587
BstEII GGTNACC 1 cut(s) 595
BstENI CCTNNNNNAGG 1 cut(s) 1180
BstF5I GGATG 5 cut(s) 449, 597, 646, 1230, 1253
BstH2I RGCGCY 1 cut(s) 478
BstHHI GCGC 1 cut(s) 477
BstKTI GATC 7 cut(s) 194, 282, 430, 695, 784, 808, 1216
BstMAI GTCTC 3 cut(s) 147, 501, 1258
BstMBI GATC 7 cut(s) 191, 279, 427, 692, 781, 805, 1213
BstMWI GCNNNNNNNGC 2 cut(s) 565, 944
BstNI CCWGG 4 cut(s) 504, 602, 865, 1177
BstNSI RCATGY 1 cut(s) 1023
BstPAI GACNNNNGTC 1 cut(s) 176
BstPI GGTNACC 1 cut(s) 595
BstSCI CCNGG 5 cut(s) 502, 600, 863, 1113, 1175
BstSLI GKGCMC 1 cut(s) 138
BstV1I GCAGC 5 cut(s) 404, 464, 544, 925, 926
BstX2I RGATCY 2 cut(s) 191, 427
BstYI RGATCY 2 cut(s) 191, 427
BstZ17I GTATAC 1 cut(s) 759
BsuI GTATCC 1 cut(s) 538
BsuRI GGCC 2 cut(s) 70, 586
BtgI CCRYGG 2 cut(s) 98, 587
BtgZI GCGATG 1 cut(s) 34
BtsCI GGATG 5 cut(s) 449, 597, 646, 1230, 1253
BtsIMutI CAGTG 3 cut(s) 180, 306, 1009
BveI ACCTGC 1 cut(s) 719
Cac8I GCNNGC 1 cut(s) 55
CfoI GCGC 1 cut(s) 477
Cfr13I GGNCC 3 cut(s) 317, 365, 1173
CseI GACGC 1 cut(s) 113
CspCI CAANNNNNGTGG 2 cut(s) 773, 808
DdeI CTNAG 6 cut(s) 72, 369, 396, 1069, 1098, 1274
DpnI GATC 7 cut(s) 193, 281, 429, 694, 783, 807, 1215
DpnII GATC 7 cut(s) 191, 279, 427, 692, 781, 805, 1213
Ecl136II GAGCTC 1 cut(s) 699
Eco130I CCWWGG 4 cut(s) 98, 587, 836, 1233
Eco24I GRGCYC 1 cut(s) 701
Eco31I GGTCTC 1 cut(s) 501
Eco47I GGWCC 3 cut(s) 317, 365, 1173
Eco47III AGCGCT 1 cut(s) 476
Eco53kI GAGCTC 1 cut(s) 699
Eco91I GGTNACC 1 cut(s) 595
EcoICRI GAGCTC 1 cut(s) 699
EcoNI CCTNNNNNAGG 1 cut(s) 1180
EcoO109I RGGNCCY 1 cut(s) 365
EcoO65I GGTNACC 1 cut(s) 595
EcoRII CCWGG 4 cut(s) 502, 600, 863, 1175
EcoT14I CCWWGG 4 cut(s) 98, 587, 836, 1233
EcoT38I GRGCYC 1 cut(s) 701
ErhI CCWWGG 4 cut(s) 98, 587, 836, 1233
FaqI GGGAC 2 cut(s) 116, 914
FauNDI CATATG 1 cut(s) 517
FbaI TGATCA 1 cut(s) 1213
FblI GTMKAC 2 cut(s) 758, 819
Fnu4HI GCNGC 5 cut(s) 393, 478, 533, 915, 939
FokI GGATG 5 cut(s) 456, 604, 653, 1237, 1260
FriOI GRGCYC 1 cut(s) 701
Fsp4HI GCNGC 5 cut(s) 393, 478, 533, 915, 939
FspBI CTAG 4 cut(s) 461, 663, 942, 1142
GlaI GCGC 1 cut(s) 476
GluI GCNGC 5 cut(s) 393, 478, 533, 915, 939
GsuI CTGGAG 2 cut(s) 444, 525
HaeII RGCGCY 1 cut(s) 478
HaeIII GGCC 2 cut(s) 70, 586
HapII CCGG 2 cut(s) 67, 1114
HgaI GACGC 1 cut(s) 113
HhaI GCGC 1 cut(s) 477
Hin6I GCGC 1 cut(s) 475
HinP1I GCGC 1 cut(s) 475
HincII GTYRAC 2 cut(s) 456, 1000
HindII GTYRAC 2 cut(s) 456, 1000
HinfI GANTC 3 cut(s) 5, 650, 703
HpaI GTTAAC 1 cut(s) 1000
HpaII CCGG 2 cut(s) 67, 1114
HphI GGTGA 7 cut(s) 197, 566, 905, 1058, 1086, 1148, 1284
Hpy166II GTNNAC 8 cut(s) 136, 340, 456, 759, 820, 973, 1000, 1033
Hpy188I TCNGA 2 cut(s) 781, 1213
Hpy188III TCNNGA 6 cut(s) 291, 323, 368, 382, 634, 1245
Hpy8I GTNNAC 8 cut(s) 136, 340, 456, 759, 820, 973, 1000, 1033
HpyAV CCTTC 7 cut(s) 210, 509, 610, 631, 793, 890, 1058
HpyCH4III ACNGT 7 cut(s) 34, 46, 310, 337, 995, 1004, 1136
HpyCH4V TGCA 7 cut(s) 136, 392, 480, 532, 581, 728, 1028
HpyF10VI GCNNNNNNNGC 2 cut(s) 565, 944
HpyF3I CTNAG 6 cut(s) 72, 369, 396, 1069, 1098, 1274
HspAI GCGC 1 cut(s) 475
Ksp22I TGATCA 1 cut(s) 1213
KspAI GTTAAC 1 cut(s) 1000
Kzo9I GATC 7 cut(s) 191, 279, 427, 692, 781, 805, 1213
LmnI GCTCC 3 cut(s) 202, 245, 922
Lsp1109I GCAGC 5 cut(s) 404, 464, 544, 925, 926
MaeI CTAG 4 cut(s) 461, 663, 942, 1142
MaeIII GTNAC 5 cut(s) 169, 178, 595, 875, 1130
MalI GATC 7 cut(s) 193, 281, 429, 694, 783, 807, 1215
MboI GATC 7 cut(s) 191, 279, 427, 692, 781, 805, 1213
MboII GAAGA 1 cut(s) 1291
MfeI CAATTG 1 cut(s) 204
MflI RGATCY 2 cut(s) 191, 427
MhlI GDGCHC 4 cut(s) 138, 701, 736, 1089
MluCI AATT 5 cut(s) 92, 204, 405, 658, 1153
MseI TTAA 2 cut(s) 26, 999
MslI CAYNNNNRTG 5 cut(s) 167, 891, 978, 1099, 1185
MspI CCGG 2 cut(s) 67, 1114
MspR9I CCNGG 5 cut(s) 504, 602, 865, 1115, 1177
MunI CAATTG 1 cut(s) 204
MvaI CCWGG 4 cut(s) 504, 602, 865, 1177
MwoI GCNNNNNNNGC 2 cut(s) 565, 944
NciI CCSGG 1 cut(s) 1115
NcoI CCATGG 2 cut(s) 98, 587
NdeI CATATG 1 cut(s) 517
NdeII GATC 7 cut(s) 191, 279, 427, 692, 781, 805, 1213
NlaIV GGNNCC 1 cut(s) 924
NmuCI GTSAC 3 cut(s) 169, 178, 1130
NspI RCATGY 1 cut(s) 1023
OliI CACNNNNGTG 1 cut(s) 1099
PaqCI CACCTGC 1 cut(s) 719
PasI CCCWGGG 1 cut(s) 601
PciI ACATGT 1 cut(s) 1019
PcsI WCGNNNNNNNCGW 1 cut(s) 991
PfeI GAWTC 3 cut(s) 5, 650, 703
PflFI GACNNNGTC 2 cut(s) 363, 451
PfoI TCCNGGA 2 cut(s) 502, 1113
PkrI GCNGC 5 cut(s) 394, 479, 534, 916, 940
PpuMI RGGWCCY 1 cut(s) 365
PscI ACATGT 1 cut(s) 1019
PshAI GACNNNNGTC 1 cut(s) 176
Psp124BI GAGCTC 1 cut(s) 701
Psp5II RGGWCCY 1 cut(s) 365
Psp6I CCWGG 4 cut(s) 502, 600, 863, 1175
PspEI GGTNACC 1 cut(s) 595
PspGI CCWGG 4 cut(s) 502, 600, 863, 1175
PspN4I GGNNCC 1 cut(s) 924
PspPI GGNCC 3 cut(s) 317, 365, 1173
PspPPI RGGWCCY 1 cut(s) 365
PsrI GAACNNNNNNTAC 2 cut(s) 843, 875
PsuI RGATCY 2 cut(s) 191, 427
PsyI GACNNNGTC 2 cut(s) 363, 451
RseI CAYNNNNRTG 5 cut(s) 167, 891, 978, 1099, 1185
SacI GAGCTC 1 cut(s) 701
SaqAI TTAA 2 cut(s) 26, 999
SatI GCNGC 5 cut(s) 393, 478, 533, 915, 939
Sau3AI GATC 7 cut(s) 191, 279, 427, 692, 781, 805, 1213
Sau96I GGNCC 3 cut(s) 317, 365, 1173
ScrFI CCNGG 5 cut(s) 504, 602, 865, 1115, 1177
SduI GDGCHC 4 cut(s) 138, 701, 736, 1089
SinI GGWCC 3 cut(s) 317, 365, 1173
SmiMI CAYNNNNRTG 5 cut(s) 167, 891, 978, 1099, 1185
SmlI CTYRAG 2 cut(s) 249, 380
SmoI CTYRAG 2 cut(s) 249, 380
Sse9I AATT 5 cut(s) 92, 204, 405, 658, 1153
SspI AATATT 1 cut(s) 776
SspMI CTAG 4 cut(s) 461, 663, 942, 1142
SstI GAGCTC 1 cut(s) 701
StyD4I CCNGG 5 cut(s) 502, 600, 863, 1113, 1175
StyI CCWWGG 4 cut(s) 98, 587, 836, 1233
TaaI ACNGT 7 cut(s) 34, 46, 310, 337, 995, 1004, 1136
TaqI TCGA 5 cut(s) 8, 471, 695, 701, 989
TaqII GACCGA 1 cut(s) 837
TasI AATT 5 cut(s) 92, 204, 405, 658, 1153
TfiI GAWTC 3 cut(s) 5, 650, 703
Tru1I TTAA 2 cut(s) 26, 999
Tru9I TTAA 2 cut(s) 26, 999
TscAI CASTG 3 cut(s) 187, 313, 1009
TseFI GTSAC 3 cut(s) 169, 178, 1130
TseI GCWGC 5 cut(s) 392, 477, 532, 914, 938
Tsp45I GTSAC 3 cut(s) 169, 178, 1130
TspDTI ATGAA 6 cut(s) 255, 482, 504, 786, 845, 996
TspGWI ACGGA 1 cut(s) 232
TspRI CASTG 3 cut(s) 187, 313, 1009
Tth111I GACNNNGTC 2 cut(s) 363, 451
VneI GTGCAC 1 cut(s) 134
VpaK11BI GGWCC 3 cut(s) 317, 365, 1173
XagI CCTNNNNNAGG 1 cut(s) 1180
XceI RCATGY 1 cut(s) 1023
XcmI CCANNNNNNNNNTGG 2 cut(s) 311, 1040
XmiI GTMKAC 2 cut(s) 758, 819
XspI CTAG 4 cut(s) 461, 663, 942, 1142
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.