pycom11g19470

Purple acid phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0005127
Physical Location & Seq
Forward (+)
22018440 .. 22022774
4335 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom11g19470.1

Sequence Viewer

Length: 1305 bp
ATGGACCCCAAGCAGATAATCACGCTCCTTTTCTTAATCTCCGCCACCGTCATCTCCGCCCATGAATACCTCCGACCACCGCCTCGCAAGGCCCTCCATTTCCCATGGAGTCGAAAATCCTCTTCTCTTCCCCAGCAGGTCCACATCTCTTTGGCAGGAGACAAGCACATGCGAGTGACGTGGGTCACTGATGATAAATATGCTCCTTCAGTTGTCGAATATGGAACATCATCTGGGACATACAGTTCTGTAGCTCAAGGAGAAAGCACCTCTTATACGTATATGCTCTATAGCTCAGGAAAGATACACCATACTGTCATTGGGCCTCTTGAACATGACACCGTCTACTTCTACCAATGTGGAGGCCAAGGTCCTGAGTTTCAGTTCAAGACCCCTCCAGCTCAATTTCCAATTACTTTTGCTGTGGCTGGAGATCTCGGACAAACTGGATGGACAAAGTCAACCCTAGACCACATTGACCAGTGCAAATATGATGTGCATCTGCTTCCTGGAGACCTTTCATATGCTGATTACATTCAGTCTCGGTGGGATACATTTGGTGAGCTGGTGCAGCCACTTGCAAGTGCAAGGCCATGGATGGTAACCCAAGGGAACCATGAAAGGGAAAATATACCATTGTTAAAGGATGGGTTTCAATCCTATAATTCCAGGTGGAAAATGCCGTATGAAGAAAGCGGATCAAGTTCAAAGCTTTACTATTCCTTCGAACTTGCAGGTGTCCATGCTATCATGCTTGGTTCATATACTGATTATGATGAGTATTCAGGTCAATACAGGTGGTTGAAGGCTGATCTTTCAAAGGTTGACCGACAGAAGACGCCTTGGCTGCTCGTGCTATTTCATGTTCCCTGGTACAATAGTAACAAGGCTCATCAAGGTGAAGGGGACAGCATGATGGCAGCAATGGAGCCGTTGCTTTATGCTGCCAGTGTGGATATAGTGTTTGCTGGTCATGTTCATGCTTATGAACGGACGAAACGTGTGAACAATGGAAGATCAGATACATGCGGTGCTGTCCACATAACCATTGGTGATGGCGGAAACAGAGAAGGGTTAGCTCACAAGTATAAAAGGCCATCACCTGAGTGGTCAGTTTTCCGGGAAGCAAGTTTTGGTCATGGTGAGCTGAATATAAAGAACTCGACCCATGCCTTATGGACCTGGCATAGGAATGACGACGACGAGCCTGTAAGATCCGATCAGGTCTGGATAACCTCGTTGGTTAGTTCGGGATGCGTTGCTGAGAAGAGACATGAACAAAGGAAGATACTCATCGAACCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003993 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0009987 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0042578 GO:0044237
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metallophos Metallophos_C Pur_ac_phosph_N

Protein Analysis

435

Amino Acids

49.19

Weight (kDa)

6.39

Isoelectric Point (pI)

44.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pur_ac_phosph_N PF16656 44 - 131 7.3e-22 Purple acid Phosphatase, N-terminal domain
Metallophos PF00149 139 - 329 1.2e-18 Calcineurin-like phosphoesterase
Metallophos_C PF14008 345 - 401 3.5e-21 Iron/zinc purple acid phosphatase-like protein C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 725
Acc36I ACCTGC 2 cut(s) 127, 725
AccI GTMKAC 1 cut(s) 345
AciI CCGC 6 cut(s) 42, 57, 80, 696, 1029, 1059
AclWI GGATC 2 cut(s) 706, 1209
AcuI CTGAAG 1 cut(s) 192
AcyI GRCGYC 1 cut(s) 839
AfaI GTAC 1 cut(s) 875
AfiI CCNNNNNNNGG 2 cut(s) 622, 1188
AflIII ACRYGT 1 cut(s) 1000
AgsI TTSAA 6 cut(s) 332, 388, 656, 708, 805, 819
AjiI CACGTC 1 cut(s) 180
AjnI CCWGG 4 cut(s) 508, 668, 869, 1181
AjuI GAANNNNNNNTTGG 2 cut(s) 1116, 1148
AleI CACNNNNGTG 1 cut(s) 1105
AloI GAACNNNNNNTCC 2 cut(s) 229, 261
AluBI AGCT 7 cut(s) 254, 294, 401, 565, 712, 1079, 1147
AluI AGCT 7 cut(s) 254, 294, 401, 565, 712, 1079, 1147
Alw26I GTCTC 4 cut(s) 153, 507, 546, 1264
AlwI GGATC 2 cut(s) 706, 1209
AoxI GGCC 5 cut(s) 90, 323, 364, 590, 1094
ApeKI GCWGC 4 cut(s) 571, 847, 920, 944
AspS9I GGNCC 6 cut(s) 4, 91, 139, 323, 371, 1179
AsuC2I CCSGG 1 cut(s) 1121
AsuHPI GGTGA 5 cut(s) 572, 911, 1064, 1092, 1154
AsuII TTCGAA 1 cut(s) 726
AvaII GGWCC 4 cut(s) 4, 139, 371, 1179
BaeI ACNNNNGTAYC 2 cut(s) 865, 898
BarI GAAGNNNNNNTAC 2 cut(s) 1006, 1038
BauI CACGAG 1 cut(s) 851
BbsI GAAGAC 1 cut(s) 842
BbvI GCAGC 4 cut(s) 583, 834, 931, 932
BccI CCATC 6 cut(s) 444, 592, 641, 910, 1049, 1105
BceAI ACGGC 2 cut(s) 667, 916
BciT130I CCWGG 4 cut(s) 510, 670, 871, 1183
BciVI GTATCC 1 cut(s) 544
BcnI CCSGG 1 cut(s) 1121
BcoDI GTCTC 4 cut(s) 153, 507, 546, 1264
BfaI CTAG 1 cut(s) 467
BfmI CTRYAG 2 cut(s) 249, 289
BfuAI ACCTGC 2 cut(s) 127, 725
BfuI GTATCC 1 cut(s) 544
BglII AGATCT 1 cut(s) 433
BisI GCNGC 4 cut(s) 572, 848, 921, 945
BlsI GCNGC 4 cut(s) 573, 849, 922, 946
Bme1390I CCNGG 5 cut(s) 510, 670, 871, 1121, 1183
Bme18I GGWCC 4 cut(s) 4, 139, 371, 1179
BmgBI CACGTC 1 cut(s) 180
BmgT120I GGNCC 6 cut(s) 4, 91, 139, 323, 371, 1179
BmiI GGNNCC 3 cut(s) 6, 614, 930
BmrFI CCNGG 5 cut(s) 510, 670, 871, 1121, 1183
BmsI GCATC 2 cut(s) 508, 1244
BoxI GACNNNNGTC 1 cut(s) 182
BpiI GAAGAC 1 cut(s) 842
BpmI CTGGAG 3 cut(s) 381, 450, 531
Bpu10I CCTNAGC 1 cut(s) 295
Bpu14I TTCGAA 1 cut(s) 726
BpuEI CTTGAG 1 cut(s) 240
BpuMI CCSGG 1 cut(s) 1121
BsaAI YACGTR 1 cut(s) 279
BsaBI GATNNNNATC 2 cut(s) 498, 1292
BsaHI GRCGYC 1 cut(s) 839
BsaI GGTCTC 1 cut(s) 507
BsaJI CCNNGG 6 cut(s) 104, 367, 593, 607, 842, 869
Bsc4I CCNNNNNNNGG 2 cut(s) 622, 1188
Bse1I ACTGG 3 cut(s) 451, 481, 948
Bse3DI GCAATG 1 cut(s) 930
Bse8I GATNNNNATC 2 cut(s) 498, 1292
BseBI CCWGG 4 cut(s) 510, 670, 871, 1183
BseDI CCNNGG 6 cut(s) 104, 367, 593, 607, 842, 869
BseGI GGATG 4 cut(s) 455, 603, 652, 1259
BseJI GATNNNNATC 2 cut(s) 498, 1292
BseLI CCNNNNNNNGG 2 cut(s) 622, 1188
BseMI GCAATG 1 cut(s) 930
BseMII CTCAG 4 cut(s) 309, 366, 1095, 1254
BseNI ACTGG 3 cut(s) 451, 481, 948
BseXI GCAGC 4 cut(s) 583, 834, 931, 932
BseYI CCCAGC 1 cut(s) 132
BsgI GTGCAG 1 cut(s) 590
BshFI GGCC 5 cut(s) 92, 325, 366, 592, 1096
BsiSI CCGG 1 cut(s) 1120
BslFI GGGAC 2 cut(s) 250, 920
BslI CCNNNNNNNGG 2 cut(s) 622, 1188
BsmAI GTCTC 4 cut(s) 153, 507, 546, 1264
BsmFI GGGAC 2 cut(s) 250, 920
BsnI GGCC 5 cut(s) 92, 325, 366, 592, 1096
Bso31I GGTCTC 1 cut(s) 507
Bsp119I TTCGAA 1 cut(s) 726
Bsp143I GATC 6 cut(s) 433, 698, 811, 1016, 1214, 1219
Bsp19I CCATGG 2 cut(s) 104, 593
BspACI CCGC 6 cut(s) 42, 57, 80, 696, 1029, 1059
BspANI GGCC 5 cut(s) 92, 325, 366, 592, 1096
BspCNI CTCAG 4 cut(s) 308, 367, 1096, 1255
BspLI GGNNCC 3 cut(s) 6, 614, 930
BspMI ACCTGC 2 cut(s) 127, 725
BspPI GGATC 2 cut(s) 706, 1209
BspT104I TTCGAA 1 cut(s) 726
BspTNI GGTCTC 1 cut(s) 507
BsrDI GCAATG 1 cut(s) 930
BsrI ACTGG 3 cut(s) 451, 481, 948
BssECI CCNNGG 6 cut(s) 104, 367, 593, 607, 842, 869
BssMI GATC 6 cut(s) 433, 698, 811, 1016, 1214, 1219
BssNI GRCGYC 1 cut(s) 839
BssSI CACGAG 1 cut(s) 851
BssT1I CCWWGG 5 cut(s) 104, 367, 593, 607, 842
Bst2BI CACGAG 1 cut(s) 851
Bst2UI CCWGG 4 cut(s) 510, 670, 871, 1183
Bst4CI ACNGT 4 cut(s) 49, 245, 316, 343
Bst6I CTCTTC 3 cut(s) 127, 132, 1262
BstACI GRCGYC 1 cut(s) 839
BstBAI YACGTR 1 cut(s) 279
BstBI TTCGAA 1 cut(s) 726
BstDEI CTNAG 4 cut(s) 295, 375, 1104, 1263
BstDSI CCRYGG 2 cut(s) 104, 593
BstEII GGTNACC 1 cut(s) 601
BstENI CCTNNNNNAGG 1 cut(s) 1186
BstF5I GGATG 4 cut(s) 455, 603, 652, 1259
BstKTI GATC 6 cut(s) 436, 701, 814, 1019, 1217, 1222
BstMAI GTCTC 4 cut(s) 153, 507, 546, 1264
BstMBI GATC 6 cut(s) 433, 698, 811, 1016, 1214, 1219
BstMWI GCNNNNNNNGC 3 cut(s) 571, 847, 853
BstNI CCWGG 4 cut(s) 510, 670, 871, 1183
BstNSI RCATGY 2 cut(s) 172, 1029
BstPAI GACNNNNGTC 1 cut(s) 182
BstPI GGTNACC 1 cut(s) 601
BstSCI CCNGG 5 cut(s) 508, 668, 869, 1119, 1181
BstSFI CTRYAG 2 cut(s) 249, 289
BstSNI TACGTA 1 cut(s) 279
BstV1I GCAGC 4 cut(s) 583, 834, 931, 932
BstV2I GAAGAC 1 cut(s) 842
BstX2I RGATCY 2 cut(s) 433, 1214
BstYI RGATCY 2 cut(s) 433, 1214
BsuI GTATCC 1 cut(s) 544
BsuRI GGCC 5 cut(s) 92, 325, 366, 592, 1096
BtgI CCRYGG 2 cut(s) 104, 593
BtrI CACGTC 1 cut(s) 180
BtsCI GGATG 4 cut(s) 455, 603, 652, 1259
BtsIMutI CAGTG 3 cut(s) 186, 488, 955
BveI ACCTGC 2 cut(s) 127, 725
Cfr13I GGNCC 6 cut(s) 4, 91, 139, 323, 371, 1179
CseI GACGC 1 cut(s) 847
Csp6I GTAC 1 cut(s) 874
CspCI CAANNNNNGTGG 2 cut(s) 779, 814
CviQI GTAC 1 cut(s) 874
DdeI CTNAG 4 cut(s) 295, 375, 1104, 1263
DpnI GATC 6 cut(s) 435, 700, 813, 1018, 1216, 1221
DpnII GATC 6 cut(s) 433, 698, 811, 1016, 1214, 1219
Eam1104I CTCTTC 3 cut(s) 127, 132, 1262
EarI CTCTTC 3 cut(s) 127, 132, 1262
EciI GGCGGA 3 cut(s) 31, 46, 1074
Eco105I TACGTA 1 cut(s) 279
Eco130I CCWWGG 5 cut(s) 104, 367, 593, 607, 842
Eco31I GGTCTC 1 cut(s) 507
Eco47I GGWCC 4 cut(s) 4, 139, 371, 1179
Eco57I CTGAAG 1 cut(s) 192
Eco91I GGTNACC 1 cut(s) 601
EcoNI CCTNNNNNAGG 1 cut(s) 1186
EcoO109I RGGNCCY 2 cut(s) 91, 371
EcoO65I GGTNACC 1 cut(s) 601
EcoRII CCWGG 4 cut(s) 508, 668, 869, 1181
EcoT14I CCWWGG 5 cut(s) 104, 367, 593, 607, 842
ErhI CCWWGG 5 cut(s) 104, 367, 593, 607, 842
FalI AAGNNNNNCTT 2 cut(s) 256, 288
FaqI GGGAC 2 cut(s) 250, 920
FauNDI CATATG 1 cut(s) 523
FblI GTMKAC 1 cut(s) 345
Fnu4HI GCNGC 4 cut(s) 572, 848, 921, 945
FokI GGATG 4 cut(s) 462, 610, 659, 1266
Fsp4HI GCNGC 4 cut(s) 572, 848, 921, 945
FspBI CTAG 1 cut(s) 467
GluI GCNGC 4 cut(s) 572, 848, 921, 945
GsaI CCCAGC 1 cut(s) 136
GsuI CTGGAG 3 cut(s) 381, 450, 531
HaeIII GGCC 5 cut(s) 92, 325, 366, 592, 1096
HapII CCGG 1 cut(s) 1120
HgaI GACGC 1 cut(s) 847
Hin1I GRCGYC 1 cut(s) 839
HincII GTYRAC 2 cut(s) 462, 826
HindII GTYRAC 2 cut(s) 462, 826
HindIII AAGCTT 1 cut(s) 710
HinfI GANTC 1 cut(s) 109
HpaII CCGG 1 cut(s) 1120
HphI GGTGA 5 cut(s) 572, 911, 1064, 1092, 1154
Hpy166II GTNNAC 6 cut(s) 142, 346, 462, 826, 1006, 1039
Hpy188I TCNGA 4 cut(s) 74, 440, 1021, 1219
Hpy188III TCNNGA 6 cut(s) 297, 329, 374, 388, 1228, 1251
Hpy8I GTNNAC 6 cut(s) 142, 346, 462, 826, 1006, 1039
Hpy99I CGWCG 2 cut(s) 1202, 1205
HpyAV CCTTC 5 cut(s) 216, 733, 799, 896, 1064
HpyCH4III ACNGT 4 cut(s) 49, 245, 316, 343
HpyCH4IV ACGT 3 cut(s) 179, 278, 1000
HpyCH4V TGCA 6 cut(s) 486, 499, 571, 581, 587, 734
HpyF10VI GCNNNNNNNGC 3 cut(s) 571, 847, 853
HpyF3I CTNAG 4 cut(s) 295, 375, 1104, 1263
HpySE526I ACGT 3 cut(s) 179, 278, 1000
Hsp92I GRCGYC 1 cut(s) 839
Kzo9I GATC 6 cut(s) 433, 698, 811, 1016, 1214, 1219
LmnI GCTCC 3 cut(s) 30, 208, 928
Lsp1109I GCAGC 4 cut(s) 583, 834, 931, 932
LweI GCATC 2 cut(s) 508, 1244
MaeI CTAG 1 cut(s) 467
MaeII ACGT 3 cut(s) 179, 278, 1000
MaeIII GTNAC 4 cut(s) 175, 184, 601, 881
MalI GATC 6 cut(s) 435, 700, 813, 1018, 1216, 1221
MboI GATC 6 cut(s) 433, 698, 811, 1016, 1214, 1219
MboII GAAGA 7 cut(s) 114, 119, 701, 847, 1026, 1279, 1297
MflI RGATCY 2 cut(s) 433, 1214
MluCI AATT 3 cut(s) 404, 411, 664
MlyI GAGTC 1 cut(s) 118
MmeI TCCRAC 1 cut(s) 97
MnlI CCTC 9 cut(s) 80, 93, 104, 130, 280, 336, 356, 405, 1246
MseI TTAA 2 cut(s) 35, 641
MslI CAYNNNNRTG 6 cut(s) 173, 897, 978, 984, 1105, 1191
MspI CCGG 1 cut(s) 1120
MspR9I CCNGG 5 cut(s) 510, 670, 871, 1121, 1183
MvaI CCWGG 4 cut(s) 510, 670, 871, 1183
MwoI GCNNNNNNNGC 3 cut(s) 571, 847, 853
NciI CCSGG 1 cut(s) 1121
NcoI CCATGG 2 cut(s) 104, 593
NdeI CATATG 1 cut(s) 523
NdeII GATC 6 cut(s) 433, 698, 811, 1016, 1214, 1219
NlaIV GGNNCC 3 cut(s) 6, 614, 930
NmuCI GTSAC 2 cut(s) 175, 184
NspI RCATGY 2 cut(s) 172, 1029
NspV TTCGAA 1 cut(s) 726
OliI CACNNNNGTG 1 cut(s) 1105
PaqCI CACCTGC 1 cut(s) 725
PcsI WCGNNNNNNNCGW 1 cut(s) 997
PflFI GACNNNGTC 2 cut(s) 341, 457
PfoI TCCNGGA 2 cut(s) 508, 1119
PkrI GCNGC 4 cut(s) 573, 849, 922, 946
PleI GAGTC 1 cut(s) 117
PpsI GAGTC 1 cut(s) 117
Ppu21I YACGTR 1 cut(s) 279
PpuMI RGGWCCY 1 cut(s) 371
PshAI GACNNNNGTC 1 cut(s) 182
Psp5II RGGWCCY 1 cut(s) 371
Psp6I CCWGG 4 cut(s) 508, 668, 869, 1181
PspEI GGTNACC 1 cut(s) 601
PspFI CCCAGC 1 cut(s) 132
PspGI CCWGG 4 cut(s) 508, 668, 869, 1181
PspN4I GGNNCC 3 cut(s) 6, 614, 930
PspPI GGNCC 6 cut(s) 4, 91, 139, 323, 371, 1179
PspPPI RGGWCCY 1 cut(s) 371
PsuI RGATCY 2 cut(s) 433, 1214
PsyI GACNNNGTC 2 cut(s) 341, 457
RsaI GTAC 1 cut(s) 875
RsaNI GTAC 1 cut(s) 874
RseI CAYNNNNRTG 6 cut(s) 173, 897, 978, 984, 1105, 1191
SaqAI TTAA 2 cut(s) 35, 641
SatI GCNGC 4 cut(s) 572, 848, 921, 945
Sau3AI GATC 6 cut(s) 433, 698, 811, 1016, 1214, 1219
Sau96I GGNCC 6 cut(s) 4, 91, 139, 323, 371, 1179
SchI GAGTC 1 cut(s) 118
ScrFI CCNGG 5 cut(s) 510, 670, 871, 1121, 1183
SfaNI GCATC 2 cut(s) 508, 1244
SfcI CTRYAG 2 cut(s) 249, 289
SfuI TTCGAA 1 cut(s) 726
SinI GGWCC 4 cut(s) 4, 139, 371, 1179
SmiMI CAYNNNNRTG 6 cut(s) 173, 897, 978, 984, 1105, 1191
SmlI CTYRAG 1 cut(s) 255
SmoI CTYRAG 1 cut(s) 255
SnaBI TACGTA 1 cut(s) 279
Sse9I AATT 3 cut(s) 404, 411, 664
SsiI CCGC 6 cut(s) 42, 57, 80, 696, 1029, 1059
SspMI CTAG 1 cut(s) 467
StyD4I CCNGG 5 cut(s) 508, 668, 869, 1119, 1181
StyI CCWWGG 5 cut(s) 104, 367, 593, 607, 842
TaaI ACNGT 4 cut(s) 49, 245, 316, 343
TaiI ACGT 3 cut(s) 182, 281, 1003
TaqI TCGA 5 cut(s) 112, 216, 726, 1163, 1296
TaqII GACCGA 1 cut(s) 843
TasI AATT 3 cut(s) 404, 411, 664
Tru1I TTAA 2 cut(s) 35, 641
Tru9I TTAA 2 cut(s) 35, 641
TscAI CASTG 3 cut(s) 193, 488, 955
TseFI GTSAC 2 cut(s) 175, 184
TseI GCWGC 4 cut(s) 571, 847, 920, 944
Tsp45I GTSAC 2 cut(s) 175, 184
TspDTI ATGAA 9 cut(s) 78, 510, 633, 702, 750, 851, 968, 1002, 1290
TspGWI ACGGA 1 cut(s) 1006
TspRI CASTG 3 cut(s) 193, 488, 955
Tth111I GACNNNGTC 2 cut(s) 341, 457
VpaK11BI GGWCC 4 cut(s) 4, 139, 371, 1179
XagI CCTNNNNNAGG 1 cut(s) 1186
XceI RCATGY 2 cut(s) 172, 1029
XcmI CCANNNNNNNNNTGG 3 cut(s) 317, 1046, 1104
XmiI GTMKAC 1 cut(s) 345
XspI CTAG 1 cut(s) 467
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.