Rroxscaffold_1G00041930

Purple acid phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0005127
Physical Location & Seq
Forward (+)
59933046 .. 59937538
4493 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00041930.1

Sequence Viewer

Length: 1065 bp
ATGGTTTGGCTGGGAAAGAAACAATGGAATCGAAGCTCTTTTTGTTGTTCATCACGGTGGGCTTCACAGTCATCGCAGGCGAGTATGTACGGCCTCAGCCTCGCAAAGACCTTCAATTCCCATGGGACGCAAAACCCTCTTCCCAACCTCAACAGTTATTTTTCTGTTAACTTCTACAAATGTGAAGGTCAAGGTCCTGAGTTTCAGCTCAAGACCCCTGCAGCTCAGTTTCCAATTACTTTTGCTGTGGTAGGAGATCTTGGTCAAACTGGATGGACAAAGTCAACCCTAGACCACATCGAGCGCTGCAAGTATGATGTGCATCTCCTTCCTGGAGACCTTTCATATGCTGATTATTTGCAGCCTCGGTGGGATACCTTTGGTGAGCTGGTTCAGCCACTCGCAAGTGCAAGGCCATGGATGGTAACCCAGGGAAACCATGAGAAGGAGAGCATACCATTCTTGAAGGATGGGTTTGAATCCTATAATTCTAGGTGGAAAATGCCGTATAAAGAGAGTGGATTGAGCTCGAATCTTTATTACTCCTTTGAAGTTGCAGGTGCTCATATTATCATGCTTGGCTCGTACACTGATTATGATGAATATTCAGATCAATACAAGTGGTTGAAGGGGTTGTTTAGTAACAAGGCTCATCAAGGTGAAGGGGACAGCATGATGGCAGCAATGGAGCCATTGCTTTATGCTGCTAGTGCTGATATAGTGTTTGCTGGTCATGTTCACGCTTATGAACGCTCGAACCGTGTTAACAGTGGAAAATCAAATACATTCAGTGCAGTTCACATTACCATTGGTGATGGAGGAAATAGAGAAGGTTTAGCTCAAAGGTATGAGCACTCGTCACCTGAGTGGTCAGTTTTCCGGGAAGCAAGTTTTGGTCACAGTGAGCTAGAGACAGTCAATTCAACCCATGCCTTTTGGACCTGGCATAGGAATGATGATGATGAGTCTGTAAGGTCTGACCAGGTATGGATGACCTCCCTGGTCAGTTCGGGATGTGTTGCTGAAAAGAGACATGAGCTGAGGAAGATACTAATGTCACCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003993 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0009987 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0042578 GO:0044237
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metallophos Metallophos_C Pur_ac_phosph_N

Protein Analysis

354

Amino Acids

40.11

Weight (kDa)

6.05

Isoelectric Point (pI)

48.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Metallophos PF00149 80 - 249 5.1e-13 Calcineurin-like phosphoesterase
Metallophos_C PF14008 266 - 324 2.8e-19 Iron/zinc purple acid phosphatase-like protein C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 548
Acc36I ACCTGC 1 cut(s) 548
AfaI GTAC 2 cut(s) 89, 587
AfeI AGCGCT 1 cut(s) 305
AfiI CCNNNNNNNGG 2 cut(s) 445, 948
AgsI TTSAA 6 cut(s) 115, 466, 479, 551, 628, 924
AjnI CCWGG 5 cut(s) 331, 429, 941, 981, 999
AjuI GAANNNNNNNTTGG 2 cut(s) 876, 908
AleI CACNNNNGTG 1 cut(s) 865
AluBI AGCT 8 cut(s) 36, 208, 224, 388, 528, 839, 907, 1039
AluI AGCT 8 cut(s) 36, 208, 224, 388, 528, 839, 907, 1039
Alw21I GWGCWC 3 cut(s) 530, 565, 855
Alw26I GTCTC 3 cut(s) 330, 905, 1024
Aor51HI AGCGCT 1 cut(s) 305
AoxI GGCC 2 cut(s) 91, 413
ApeKI GCWGC 5 cut(s) 221, 306, 361, 680, 704
AspLEI GCGC 1 cut(s) 306
AspS9I GGNCC 2 cut(s) 194, 939
AsuC2I CCSGG 1 cut(s) 881
AsuHPI GGTGA 5 cut(s) 395, 671, 824, 852, 1050
AvaII GGWCC 2 cut(s) 194, 939
BaeI ACNNNNGTAYC 1 cut(s) 1040
BanII GRGCYC 1 cut(s) 530
Bbv12I GWGCWC 3 cut(s) 530, 565, 855
BbvCI CCTCAGC 2 cut(s) 95, 1040
BbvI GCAGC 5 cut(s) 233, 293, 373, 691, 692
BccI CCATC 5 cut(s) 267, 415, 464, 670, 809
BceAI ACGGC 2 cut(s) 106, 490
BciT130I CCWGG 5 cut(s) 333, 431, 943, 983, 1001
BciVI GTATCC 1 cut(s) 367
BcnI CCSGG 1 cut(s) 881
BcoDI GTCTC 3 cut(s) 330, 905, 1024
BfaI CTAG 4 cut(s) 290, 492, 708, 908
BfmI CTRYAG 1 cut(s) 219
BfoI RGCGCY 1 cut(s) 307
BfuAI ACCTGC 1 cut(s) 548
BfuI GTATCC 1 cut(s) 367
BglII AGATCT 1 cut(s) 256
BisI GCNGC 5 cut(s) 222, 307, 362, 681, 705
BlsI GCNGC 5 cut(s) 223, 308, 363, 682, 706
Bme1390I CCNGG 6 cut(s) 333, 431, 881, 943, 983, 1001
Bme18I GGWCC 2 cut(s) 194, 939
BmgT120I GGNCC 2 cut(s) 194, 939
BmiI GGNNCC 1 cut(s) 690
BmrFI CCNGG 6 cut(s) 333, 431, 881, 943, 983, 1001
BmsI GCATC 1 cut(s) 331
BpmI CTGGAG 1 cut(s) 354
Bpu10I CCTNAGC 2 cut(s) 95, 1040
BpuEI CTTGAG 1 cut(s) 194
BpuMI CCSGG 1 cut(s) 881
BsaBI GATNNNNATC 1 cut(s) 321
BsaI GGTCTC 1 cut(s) 330
BsaJI CCNNGG 6 cut(s) 121, 365, 416, 429, 430, 999
Bsc4I CCNNNNNNNGG 2 cut(s) 445, 948
Bse1I ACTGG 1 cut(s) 274
Bse3DI GCAATG 2 cut(s) 690, 692
Bse8I GATNNNNATC 1 cut(s) 321
BseBI CCWGG 5 cut(s) 333, 431, 943, 983, 1001
BseDI CCNNGG 6 cut(s) 121, 365, 416, 429, 430, 999
BseGI GGATG 5 cut(s) 278, 426, 475, 996, 1019
BseJI GATNNNNATC 1 cut(s) 321
BseLI CCNNNNNNNGG 2 cut(s) 445, 948
BseMI GCAATG 2 cut(s) 690, 692
BseMII CTCAG 5 cut(s) 109, 189, 239, 855, 1031
BseNI ACTGG 1 cut(s) 274
BseXI GCAGC 5 cut(s) 233, 293, 373, 691, 692
BseYI CCCAGC 1 cut(s) 10
BsgI GTGCAG 1 cut(s) 813
BshFI GGCC 2 cut(s) 93, 415
BsiHKAI GWGCWC 3 cut(s) 530, 565, 855
BsiSI CCGG 1 cut(s) 880
BslFI GGGAC 2 cut(s) 139, 680
BslI CCNNNNNNNGG 2 cut(s) 445, 948
BsmAI GTCTC 3 cut(s) 330, 905, 1024
BsmFI GGGAC 2 cut(s) 139, 680
BsnI GGCC 2 cut(s) 93, 415
Bso31I GGTCTC 1 cut(s) 330
Bsp1286I GDGCHC 3 cut(s) 530, 565, 855
Bsp143I GATC 2 cut(s) 256, 610
Bsp19I CCATGG 2 cut(s) 121, 416
BspANI GGCC 2 cut(s) 93, 415
BspCNI CTCAG 5 cut(s) 108, 190, 238, 856, 1032
BspLI GGNNCC 1 cut(s) 690
BspMAI CTGCAG 1 cut(s) 223
BspMI ACCTGC 1 cut(s) 548
BspTNI GGTCTC 1 cut(s) 330
BsrDI GCAATG 2 cut(s) 690, 692
BsrI ACTGG 1 cut(s) 274
BssECI CCNNGG 6 cut(s) 121, 365, 416, 429, 430, 999
BssMI GATC 2 cut(s) 256, 610
BssT1I CCWWGG 2 cut(s) 121, 416
Bst2UI CCWGG 5 cut(s) 333, 431, 943, 983, 1001
Bst4CI ACNGT 7 cut(s) 57, 69, 155, 761, 770, 902, 916
Bst6I CTCTTC 1 cut(s) 144
BstC8I GCNNGC 1 cut(s) 78
BstDEI CTNAG 5 cut(s) 95, 198, 225, 864, 1040
BstDSI CCRYGG 2 cut(s) 121, 416
BstEII GGTNACC 1 cut(s) 424
BstENI CCTNNNNNAGG 1 cut(s) 946
BstF5I GGATG 5 cut(s) 278, 426, 475, 996, 1019
BstH2I RGCGCY 1 cut(s) 307
BstHHI GCGC 1 cut(s) 306
BstKTI GATC 2 cut(s) 259, 613
BstMAI GTCTC 3 cut(s) 330, 905, 1024
BstMBI GATC 2 cut(s) 256, 610
BstMWI GCNNNNNNNGC 2 cut(s) 394, 710
BstNI CCWGG 5 cut(s) 333, 431, 943, 983, 1001
BstPI GGTNACC 1 cut(s) 424
BstSCI CCNGG 6 cut(s) 331, 429, 879, 941, 981, 999
BstSFI CTRYAG 1 cut(s) 219
BstV1I GCAGC 5 cut(s) 233, 293, 373, 691, 692
BstX2I RGATCY 1 cut(s) 256
BstYI RGATCY 1 cut(s) 256
BsuI GTATCC 1 cut(s) 367
BsuRI GGCC 2 cut(s) 93, 415
BtgI CCRYGG 2 cut(s) 121, 416
BtgZI GCGATG 1 cut(s) 57
BtsCI GGATG 5 cut(s) 278, 426, 475, 996, 1019
BtsIMutI CAGTG 4 cut(s) 588, 775, 796, 907
BveI ACCTGC 1 cut(s) 548
Cac8I GCNNGC 1 cut(s) 78
CfoI GCGC 1 cut(s) 306
Cfr13I GGNCC 2 cut(s) 194, 939
CseI GACGC 1 cut(s) 136
CsiI ACCWGGT 1 cut(s) 981
Csp6I GTAC 2 cut(s) 88, 586
CspCI CAANNNNNGTGG 2 cut(s) 602, 637
CviAII CATG 8 cut(s) 122, 417, 440, 574, 673, 734, 929, 1034
CviQI GTAC 2 cut(s) 88, 586
DdeI CTNAG 5 cut(s) 95, 198, 225, 864, 1040
DpnI GATC 2 cut(s) 258, 612
DpnII GATC 2 cut(s) 256, 610
Eam1104I CTCTTC 1 cut(s) 144
EarI CTCTTC 1 cut(s) 144
Ecl136II GAGCTC 1 cut(s) 528
Eco130I CCWWGG 2 cut(s) 121, 416
Eco24I GRGCYC 1 cut(s) 530
Eco31I GGTCTC 1 cut(s) 330
Eco47I GGWCC 2 cut(s) 194, 939
Eco47III AGCGCT 1 cut(s) 305
Eco53kI GAGCTC 1 cut(s) 528
Eco91I GGTNACC 1 cut(s) 424
EcoICRI GAGCTC 1 cut(s) 528
EcoNI CCTNNNNNAGG 1 cut(s) 946
EcoO109I RGGNCCY 1 cut(s) 194
EcoO65I GGTNACC 1 cut(s) 424
EcoRII CCWGG 5 cut(s) 331, 429, 941, 981, 999
EcoT14I CCWWGG 2 cut(s) 121, 416
EcoT38I GRGCYC 1 cut(s) 530
ErhI CCWWGG 2 cut(s) 121, 416
FaeI CATG 8 cut(s) 125, 420, 443, 577, 676, 737, 932, 1037
FaqI GGGAC 2 cut(s) 139, 680
FatI CATG 8 cut(s) 121, 416, 439, 573, 672, 733, 928, 1033
FauNDI CATATG 1 cut(s) 346
Fnu4HI GCNGC 5 cut(s) 222, 307, 362, 681, 705
FokI GGATG 5 cut(s) 285, 433, 482, 1003, 1026
FriOI GRGCYC 1 cut(s) 530
Fsp4HI GCNGC 5 cut(s) 222, 307, 362, 681, 705
FspBI CTAG 4 cut(s) 290, 492, 708, 908
GlaI GCGC 1 cut(s) 305
GluI GCNGC 5 cut(s) 222, 307, 362, 681, 705
GsaI CCCAGC 1 cut(s) 14
GsuI CTGGAG 1 cut(s) 354
HaeII RGCGCY 1 cut(s) 307
HaeIII GGCC 2 cut(s) 93, 415
HapII CCGG 1 cut(s) 880
HgaI GACGC 1 cut(s) 136
HhaI GCGC 1 cut(s) 306
Hin1II CATG 8 cut(s) 125, 420, 443, 577, 676, 737, 932, 1037
Hin6I GCGC 1 cut(s) 304
HinP1I GCGC 1 cut(s) 304
HincII GTYRAC 3 cut(s) 169, 285, 766
HindII GTYRAC 3 cut(s) 169, 285, 766
HinfI GANTC 4 cut(s) 28, 479, 532, 965
HpaI GTTAAC 2 cut(s) 169, 766
HpaII CCGG 1 cut(s) 880
HphI GGTGA 5 cut(s) 395, 671, 824, 852, 1050
Hpy166II GTNNAC 6 cut(s) 169, 285, 588, 739, 766, 799
Hpy188I TCNGA 2 cut(s) 610, 979
Hpy188III TCNNGA 4 cut(s) 197, 211, 463, 1011
Hpy8I GTNNAC 6 cut(s) 169, 285, 588, 739, 766, 799
HpyAV CCTTC 8 cut(s) 121, 179, 338, 439, 460, 622, 656, 824
HpyCH4III ACNGT 7 cut(s) 57, 69, 155, 761, 770, 902, 916
HpyCH4V TGCA 7 cut(s) 221, 309, 322, 361, 410, 557, 794
HpyF10VI GCNNNNNNNGC 2 cut(s) 394, 710
HpyF3I CTNAG 5 cut(s) 95, 198, 225, 864, 1040
Hsp92II CATG 8 cut(s) 125, 420, 443, 577, 676, 737, 932, 1037
HspAI GCGC 1 cut(s) 304
KspAI GTTAAC 2 cut(s) 169, 766
Kzo9I GATC 2 cut(s) 256, 610
LmnI GCTCC 1 cut(s) 688
Lsp1109I GCAGC 5 cut(s) 233, 293, 373, 691, 692
LweI GCATC 1 cut(s) 331
MabI ACCWGGT 1 cut(s) 981
MaeI CTAG 4 cut(s) 290, 492, 708, 908
MaeIII GTNAC 5 cut(s) 424, 641, 858, 896, 1056
MalI GATC 2 cut(s) 258, 612
MboI GATC 2 cut(s) 256, 610
MboII GAAGA 2 cut(s) 131, 1057
MflI RGATCY 1 cut(s) 256
MhlI GDGCHC 3 cut(s) 530, 565, 855
MluCI AATT 4 cut(s) 115, 234, 487, 919
MlyI GAGTC 1 cut(s) 974
MnlI CCTC 8 cut(s) 104, 110, 147, 158, 375, 812, 1006, 1035
MseI TTAA 2 cut(s) 168, 765
MslI CAYNNNNRTG 5 cut(s) 55, 657, 744, 865, 951
MspI CCGG 1 cut(s) 880
MspR9I CCNGG 6 cut(s) 333, 431, 881, 943, 983, 1001
MvaI CCWGG 5 cut(s) 333, 431, 943, 983, 1001
MwoI GCNNNNNNNGC 2 cut(s) 394, 710
NciI CCSGG 1 cut(s) 881
NcoI CCATGG 2 cut(s) 121, 416
NdeI CATATG 1 cut(s) 346
NdeII GATC 2 cut(s) 256, 610
NlaIII CATG 8 cut(s) 125, 420, 443, 577, 676, 737, 932, 1037
NlaIV GGNNCC 1 cut(s) 690
NmuCI GTSAC 3 cut(s) 858, 896, 1056
OliI CACNNNNGTG 1 cut(s) 865
PaqCI CACCTGC 1 cut(s) 548
PasI CCCWGGG 1 cut(s) 430
PcsI WCGNNNNNNNCGW 1 cut(s) 757
PfeI GAWTC 3 cut(s) 28, 479, 532
PflFI GACNNNGTC 1 cut(s) 280
PfoI TCCNGGA 2 cut(s) 331, 879
PkrI GCNGC 5 cut(s) 223, 308, 363, 682, 706
PleI GAGTC 1 cut(s) 973
PpsI GAGTC 1 cut(s) 973
PpuMI RGGWCCY 1 cut(s) 194
Psp124BI GAGCTC 1 cut(s) 530
Psp5II RGGWCCY 1 cut(s) 194
Psp6I CCWGG 5 cut(s) 331, 429, 941, 981, 999
PspEI GGTNACC 1 cut(s) 424
PspFI CCCAGC 1 cut(s) 10
PspGI CCWGG 5 cut(s) 331, 429, 941, 981, 999
PspN4I GGNNCC 1 cut(s) 690
PspPI GGNCC 2 cut(s) 194, 939
PspPPI RGGWCCY 1 cut(s) 194
PstI CTGCAG 1 cut(s) 223
PsuI RGATCY 1 cut(s) 256
PsyI GACNNNGTC 1 cut(s) 280
RsaI GTAC 2 cut(s) 89, 587
RsaNI GTAC 2 cut(s) 88, 586
RseI CAYNNNNRTG 5 cut(s) 55, 657, 744, 865, 951
SacI GAGCTC 1 cut(s) 530
SaqAI TTAA 2 cut(s) 168, 765
SatI GCNGC 5 cut(s) 222, 307, 362, 681, 705
Sau3AI GATC 2 cut(s) 256, 610
Sau96I GGNCC 2 cut(s) 194, 939
SchI GAGTC 1 cut(s) 974
ScrFI CCNGG 6 cut(s) 333, 431, 881, 943, 983, 1001
SduI GDGCHC 3 cut(s) 530, 565, 855
SexAI ACCWGGT 1 cut(s) 981
SfaNI GCATC 1 cut(s) 331
SfcI CTRYAG 1 cut(s) 219
SinI GGWCC 2 cut(s) 194, 939
SmiMI CAYNNNNRTG 5 cut(s) 55, 657, 744, 865, 951
SmlI CTYRAG 1 cut(s) 209
SmoI CTYRAG 1 cut(s) 209
Sse9I AATT 4 cut(s) 115, 234, 487, 919
SspI AATATT 1 cut(s) 605
SspMI CTAG 4 cut(s) 290, 492, 708, 908
SstI GAGCTC 1 cut(s) 530
StyD4I CCNGG 6 cut(s) 331, 429, 879, 941, 981, 999
StyI CCWWGG 2 cut(s) 121, 416
TaaI ACNGT 7 cut(s) 57, 69, 155, 761, 770, 902, 916
TaqI TCGA 4 cut(s) 31, 300, 530, 755
TasI AATT 4 cut(s) 115, 234, 487, 919
TfiI GAWTC 3 cut(s) 28, 479, 532
Tru1I TTAA 2 cut(s) 168, 765
Tru9I TTAA 2 cut(s) 168, 765
TscAI CASTG 4 cut(s) 595, 775, 796, 907
TseFI GTSAC 3 cut(s) 858, 896, 1056
TseI GCWGC 5 cut(s) 221, 306, 361, 680, 704
Tsp45I GTSAC 3 cut(s) 858, 896, 1056
TspDTI ATGAA 4 cut(s) 39, 333, 615, 762
TspRI CASTG 4 cut(s) 595, 775, 796, 907
Tth111I GACNNNGTC 1 cut(s) 280
VpaK11BI GGWCC 2 cut(s) 194, 939
XagI CCTNNNNNAGG 1 cut(s) 946
XspI CTAG 4 cut(s) 290, 492, 708, 908
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.