Rh5DG276500

Purple acid phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0005127
Physical Location & Seq
Reverse (-)
33826563 .. 33830565
4003 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG276500.1

Sequence Viewer

Length: 525 bp
ATGATCTATAAGTCTGGACTGATACACCACACTGTTATTGGTCCTCTTGAACATGATACGGTTTACTTCTACAAATGTGGAGGACAAGGTCCTGAGTTTCAGCTCAAGACCCCTGCAGCTCAGTTTCCAATTACTTTTGCTGTGGCAGGAGATCTTGGTCAAACTGGATGGACAAAGTCAACCCTAGACCACATCGAGCGCTGCAAGTATGATGTCCATATCCTTCCTGGAGACTTTTCATATGCTGATTATTTGCAGCCTTGGTGGGATACCTTTGGTGAGCTGGTTCAGCCACTCGCAAGTGCAAGGCCATGGATGATAACCCAGGGAAACCATGAGAAGGAGAGGATACCATTCTTGAAGGATGGGTTTGATACCTATAGGTGGAAAATGCCGTATAAAGAGAGTGGATCGAGCTCGAATCTTTATTACTCCTTTGAAGTTGCAGGTGCTCATATTATCATGCTTGGCTCGTATACTGATTATGGTGAATATTCAGATCAATACAAGTGGTTGAAGGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003993 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0009987 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0042578 GO:0044237
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metallophos Metallophos_C Pur_ac_phosph_N

Protein Analysis

174

Amino Acids

20.04

Weight (kDa)

5.75

Isoelectric Point (pI)

40.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Metallophos PF00149 45 - 171 1.2e-08 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 437
Acc36I ACCTGC 1 cut(s) 437
AccI GTMKAC 1 cut(s) 476
AclWI GGATC 1 cut(s) 418
AfeI AGCGCT 1 cut(s) 200
AfiI CCNNNNNNNGG 2 cut(s) 340, 384
AgsI TTSAA 4 cut(s) 50, 361, 440, 517
AjnI CCWGG 2 cut(s) 226, 324
AluBI AGCT 4 cut(s) 103, 119, 283, 417
AluI AGCT 4 cut(s) 103, 119, 283, 417
Alw21I GWGCWC 2 cut(s) 419, 454
Alw26I GTCTC 1 cut(s) 225
AlwI GGATC 1 cut(s) 418
Aor51HI AGCGCT 1 cut(s) 200
AoxI GGCC 1 cut(s) 308
ApeKI GCWGC 3 cut(s) 116, 201, 256
AspLEI GCGC 1 cut(s) 201
AspS9I GGNCC 2 cut(s) 41, 89
AsuHPI GGTGA 2 cut(s) 290, 500
AvaII GGWCC 2 cut(s) 41, 89
BanII GRGCYC 1 cut(s) 419
Bbv12I GWGCWC 2 cut(s) 419, 454
BbvI GCAGC 3 cut(s) 128, 188, 268
BccI CCATC 2 cut(s) 162, 359
BceAI ACGGC 1 cut(s) 379
BciT130I CCWGG 2 cut(s) 228, 326
BciVI GTATCC 2 cut(s) 262, 342
BcoDI GTCTC 1 cut(s) 225
BfaI CTAG 1 cut(s) 185
BfmI CTRYAG 2 cut(s) 114, 379
BfoI RGCGCY 1 cut(s) 202
BfuAI ACCTGC 1 cut(s) 437
BfuI GTATCC 2 cut(s) 262, 342
BglII AGATCT 1 cut(s) 151
BisI GCNGC 3 cut(s) 117, 202, 257
BlsI GCNGC 3 cut(s) 118, 203, 258
Bme1390I CCNGG 2 cut(s) 228, 326
Bme18I GGWCC 2 cut(s) 41, 89
BmgT120I GGNCC 2 cut(s) 41, 89
BmrFI CCNGG 2 cut(s) 228, 326
BpmI CTGGAG 1 cut(s) 249
BpuEI CTTGAG 1 cut(s) 89
BsaJI CCNNGG 4 cut(s) 260, 311, 324, 325
Bsc4I CCNNNNNNNGG 2 cut(s) 340, 384
Bse1I ACTGG 1 cut(s) 169
BseBI CCWGG 2 cut(s) 228, 326
BseDI CCNNGG 4 cut(s) 260, 311, 324, 325
BseGI GGATG 3 cut(s) 173, 321, 370
BseLI CCNNNNNNNGG 2 cut(s) 340, 384
BseMII CTCAG 2 cut(s) 84, 134
BseNI ACTGG 1 cut(s) 169
BseXI GCAGC 3 cut(s) 128, 188, 268
BshFI GGCC 1 cut(s) 310
BsiHKAI GWGCWC 2 cut(s) 419, 454
BslI CCNNNNNNNGG 2 cut(s) 340, 384
BsmAI GTCTC 1 cut(s) 225
BsnI GGCC 1 cut(s) 310
Bsp1286I GDGCHC 2 cut(s) 419, 454
Bsp143I GATC 4 cut(s) 3, 151, 410, 499
Bsp19I CCATGG 1 cut(s) 311
BspANI GGCC 1 cut(s) 310
BspCNI CTCAG 2 cut(s) 85, 133
BspMAI CTGCAG 1 cut(s) 118
BspMI ACCTGC 1 cut(s) 437
BspPI GGATC 1 cut(s) 418
BsrI ACTGG 1 cut(s) 169
BssECI CCNNGG 4 cut(s) 260, 311, 324, 325
BssMI GATC 4 cut(s) 3, 151, 410, 499
BssNAI GTATAC 1 cut(s) 477
BssT1I CCWWGG 2 cut(s) 260, 311
Bst1107I GTATAC 1 cut(s) 477
Bst2UI CCWGG 2 cut(s) 228, 326
Bst4CI ACNGT 2 cut(s) 34, 61
BstDEI CTNAG 2 cut(s) 93, 120
BstDSI CCRYGG 1 cut(s) 311
BstF5I GGATG 3 cut(s) 173, 321, 370
BstH2I RGCGCY 1 cut(s) 202
BstHHI GCGC 1 cut(s) 201
BstKTI GATC 4 cut(s) 6, 154, 413, 502
BstMAI GTCTC 1 cut(s) 225
BstMBI GATC 4 cut(s) 3, 151, 410, 499
BstMWI GCNNNNNNNGC 1 cut(s) 289
BstNI CCWGG 2 cut(s) 228, 326
BstSCI CCNGG 2 cut(s) 226, 324
BstSFI CTRYAG 2 cut(s) 114, 379
BstV1I GCAGC 3 cut(s) 128, 188, 268
BstX2I RGATCY 1 cut(s) 151
BstYI RGATCY 1 cut(s) 151
BstZ17I GTATAC 1 cut(s) 477
BsuI GTATCC 2 cut(s) 262, 342
BsuRI GGCC 1 cut(s) 310
BtgI CCRYGG 1 cut(s) 311
BtsCI GGATG 3 cut(s) 173, 321, 370
BtsIMutI CAGTG 1 cut(s) 30
BveI ACCTGC 1 cut(s) 437
CfoI GCGC 1 cut(s) 201
Cfr13I GGNCC 2 cut(s) 41, 89
CspCI CAANNNNNGTGG 1 cut(s) 491
CviAII CATG 4 cut(s) 53, 312, 335, 463
CviJI RGCY 8 cut(s) 103, 119, 259, 283, 292, 310, 417, 471
CviKI_1 RGCY 8 cut(s) 103, 119, 259, 283, 292, 310, 417, 471
DdeI CTNAG 2 cut(s) 93, 120
DpnI GATC 4 cut(s) 5, 153, 412, 501
DpnII GATC 4 cut(s) 3, 151, 410, 499
Ecl136II GAGCTC 1 cut(s) 417
Eco130I CCWWGG 2 cut(s) 260, 311
Eco24I GRGCYC 1 cut(s) 419
Eco47I GGWCC 2 cut(s) 41, 89
Eco47III AGCGCT 1 cut(s) 200
Eco53kI GAGCTC 1 cut(s) 417
EcoICRI GAGCTC 1 cut(s) 417
EcoO109I RGGNCCY 1 cut(s) 89
EcoRII CCWGG 2 cut(s) 226, 324
EcoT14I CCWWGG 2 cut(s) 260, 311
EcoT38I GRGCYC 1 cut(s) 419
ErhI CCWWGG 2 cut(s) 260, 311
FaeI CATG 4 cut(s) 56, 315, 338, 466
FatI CATG 4 cut(s) 52, 311, 334, 462
FauNDI CATATG 1 cut(s) 241
FblI GTMKAC 1 cut(s) 476
Fnu4HI GCNGC 3 cut(s) 117, 202, 257
FokI GGATG 3 cut(s) 180, 328, 377
FriOI GRGCYC 1 cut(s) 419
Fsp4HI GCNGC 3 cut(s) 117, 202, 257
FspBI CTAG 1 cut(s) 185
GlaI GCGC 1 cut(s) 200
GluI GCNGC 3 cut(s) 117, 202, 257
GsuI CTGGAG 1 cut(s) 249
HaeII RGCGCY 1 cut(s) 202
HaeIII GGCC 1 cut(s) 310
HhaI GCGC 1 cut(s) 201
Hin1II CATG 4 cut(s) 56, 315, 338, 466
Hin6I GCGC 1 cut(s) 199
HinP1I GCGC 1 cut(s) 199
HincII GTYRAC 1 cut(s) 180
HindII GTYRAC 1 cut(s) 180
HinfI GANTC 1 cut(s) 421
HphI GGTGA 2 cut(s) 290, 500
Hpy166II GTNNAC 3 cut(s) 64, 180, 477
Hpy188I TCNGA 1 cut(s) 499
Hpy188III TCNNGA 5 cut(s) 15, 47, 92, 106, 358
Hpy8I GTNNAC 3 cut(s) 64, 180, 477
HpyAV CCTTC 4 cut(s) 233, 334, 355, 511
HpyCH4III ACNGT 2 cut(s) 34, 61
HpyCH4V TGCA 5 cut(s) 116, 204, 256, 305, 446
HpyF10VI GCNNNNNNNGC 1 cut(s) 289
HpyF3I CTNAG 2 cut(s) 93, 120
Hsp92II CATG 4 cut(s) 56, 315, 338, 466
HspAI GCGC 1 cut(s) 199
Kzo9I GATC 4 cut(s) 3, 151, 410, 499
Lsp1109I GCAGC 3 cut(s) 128, 188, 268
MaeI CTAG 1 cut(s) 185
MalI GATC 4 cut(s) 5, 153, 412, 501
MboI GATC 4 cut(s) 3, 151, 410, 499
MflI RGATCY 1 cut(s) 151
MhlI GDGCHC 2 cut(s) 419, 454
MluCI AATT 1 cut(s) 129
MnlI CCTC 3 cut(s) 54, 74, 339
MspR9I CCNGG 2 cut(s) 228, 326
MvaI CCWGG 2 cut(s) 228, 326
MwoI GCNNNNNNNGC 1 cut(s) 289
NcoI CCATGG 1 cut(s) 311
NdeI CATATG 1 cut(s) 241
NdeII GATC 4 cut(s) 3, 151, 410, 499
NlaIII CATG 4 cut(s) 56, 315, 338, 466
PaqCI CACCTGC 1 cut(s) 437
PasI CCCWGGG 1 cut(s) 325
PfeI GAWTC 1 cut(s) 421
PflFI GACNNNGTC 2 cut(s) 87, 175
PfoI TCCNGGA 1 cut(s) 226
PkrI GCNGC 3 cut(s) 118, 203, 258
PpuMI RGGWCCY 1 cut(s) 89
Psp124BI GAGCTC 1 cut(s) 419
Psp5II RGGWCCY 1 cut(s) 89
Psp6I CCWGG 2 cut(s) 226, 324
PspGI CCWGG 2 cut(s) 226, 324
PspPI GGNCC 2 cut(s) 41, 89
PspPPI RGGWCCY 1 cut(s) 89
PstI CTGCAG 1 cut(s) 118
PsuI RGATCY 1 cut(s) 151
PsyI GACNNNGTC 2 cut(s) 87, 175
SacI GAGCTC 1 cut(s) 419
SatI GCNGC 3 cut(s) 117, 202, 257
Sau3AI GATC 4 cut(s) 3, 151, 410, 499
Sau96I GGNCC 2 cut(s) 41, 89
ScrFI CCNGG 2 cut(s) 228, 326
SduI GDGCHC 2 cut(s) 419, 454
SfcI CTRYAG 2 cut(s) 114, 379
SinI GGWCC 2 cut(s) 41, 89
SmlI CTYRAG 1 cut(s) 104
SmoI CTYRAG 1 cut(s) 104
Sse9I AATT 1 cut(s) 129
SspI AATATT 1 cut(s) 494
SspMI CTAG 1 cut(s) 185
SstI GAGCTC 1 cut(s) 419
StyD4I CCNGG 2 cut(s) 226, 324
StyI CCWWGG 2 cut(s) 260, 311
TaaI ACNGT 2 cut(s) 34, 61
TaqI TCGA 3 cut(s) 195, 413, 419
TasI AATT 1 cut(s) 129
TfiI GAWTC 1 cut(s) 421
TscAI CASTG 1 cut(s) 37
TseI GCWGC 3 cut(s) 116, 201, 256
TspDTI ATGAA 1 cut(s) 228
TspRI CASTG 1 cut(s) 37
Tth111I GACNNNGTC 2 cut(s) 87, 175
VpaK11BI GGWCC 2 cut(s) 41, 89
XcmI CCANNNNNNNNNTGG 2 cut(s) 35, 224
XmiI GTMKAC 1 cut(s) 476
XspI CTAG 1 cut(s) 185
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.