pycom03g15460

Purple acid phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0005127
Physical Location & Seq
Forward (+)
16710040 .. 16713493
3454 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g15460.2

Sequence Viewer

Length: 1305 bp
ATGGACCCAAAGCCGATCATCACGGTCCTTTTCTTAATCTCCGCCACCGTCATCGCCGCCCAAGAATACGTCCGACCGCCGCCTCGCAAGGCCCTCCATTTCCCATGGAGTCGAAAACCCTCTTCTCTTCCCCAGCAGGTGCACATCTCTTTTGCAGGAGACAAGCACATGCGGGTGACGTGGGTCACTAATGATAAATCTGCTCCTTCTGTTGTTGAATATGGAACATCATCTGGGAAATACAGTTCTGTAGCTCAAGGAGAAAGCACCTCTTATAAGTATATTCTCTATAGCTCAGGAAAGATACACCATACTGTCATTGGGCCTCTTGAACATGACACCGTTTACTTCTACCAATGCGGAGGCCAAGCTCCTGAGTTTCAGTTCAAGACCCCTCCAGCTCAATTTCCAATTACTTTTGCTGTGGCTGGAGATCTTGGACAAACTGGATGGACGAAGTCAACGCTAGACCACATTGACCAGTGCAAATATGATGTGCATCTGCTTCCTGGAGACCTTTCATACGCTGATTACATTCAGTCTCGGTGGGATACATTTGGTGAGCTGGTGCAGCCACTTGCAAGTGCAAGGCCATGGATGGTAACCCAAGGGAACCATGAAAAGGAAAATATACCATTTTTAAAGGATGGGTTTCAATCCTATAATTCCAGGTGGAAAATGCCGTATGAAGAGAGTGGATCAAGTTCAAATCTTTACTATTCCTTTGAAGTTGCAGGTGTCCATGCTATCATGCTTGGTTCATATACTGATAATGATGAGTATTCAGATCAATACAGGTGGCTGAAGGCTGATCTTGCAAAGGTTGACCGACAGAAGACACCTTGGCTGCTCGTGCTCTTTCATGTTCCCTGGTACAATAGTAACAAGGCTCATCAAGGTGAAGGGGACGGCATGATGGCAGCAATGGAGCCATTGCTTTCTGCTGCCAGTGCGGATATAGTATTTGCTGGTCATGTTCATGCTTATGAACGAACGAAACGCGTGAACAATGGAAAATCAGATAGGTGCGGTGCTGTCCACATAACCATTGGTGATGGCGGAAACAAAGAAGGCTTGGCTCACAAGTATAAAAACCCACCACCCGAGTGGTCAGTTTTCCGGGAAGCAAGTTTTGGTCATGGTGAGCTGAATATAGTGAATTCAACCCATGCCTTTTGGACCTGGCATAGGAATGATGACGACGAGCCTATTAGGTCCGATCAGGTCTGGATAACCTCGTTGGTTAGTTCAGGATGTGTTGCTGAGAAGACACATGAACTAAGGAAGATACTTACGGAACCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003993 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0009987 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0042578 GO:0044237
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metallophos Metallophos_C Pur_ac_phosph_N

Protein Analysis

435

Amino Acids

48.86

Weight (kDa)

6.28

Isoelectric Point (pI)

37.65

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 276
AarI CACCTGC 2 cut(s) 127, 725
Acc36I ACCTGC 2 cut(s) 127, 725
AccII CGCG 1 cut(s) 1002
AciI CCGC 9 cut(s) 42, 57, 77, 80, 172, 360, 953, 1029, 1059
AclWI GGATC 1 cut(s) 706
AcsI RAATTY 1 cut(s) 1159
AcuI CTGAAG 1 cut(s) 824
AfaI GTAC 1 cut(s) 875
AfiI CCNNNNNNNGG 2 cut(s) 622, 1188
AflIII ACRYGT 1 cut(s) 1000
AgsI TTSAA 7 cut(s) 218, 332, 388, 656, 708, 728, 1164
AjiI CACGTC 1 cut(s) 180
AjnI CCWGG 4 cut(s) 508, 668, 869, 1181
AjuI GAANNNNNNNTTGG 2 cut(s) 1116, 1148
AleI CACNNNNGTG 1 cut(s) 1105
AloI GAACNNNNNNTCC 2 cut(s) 229, 261
AluBI AGCT 6 cut(s) 254, 294, 371, 401, 565, 1147
AluI AGCT 6 cut(s) 254, 294, 371, 401, 565, 1147
Alw21I GWGCWC 2 cut(s) 144, 858
Alw26I GTCTC 3 cut(s) 153, 507, 546
Alw44I GTGCAC 1 cut(s) 140
AlwI GGATC 1 cut(s) 706
Ama87I CYCGRG 1 cut(s) 1103
AoxI GGCC 4 cut(s) 90, 323, 364, 590
ApaLI GTGCAC 1 cut(s) 140
ApeKI GCWGC 4 cut(s) 571, 847, 920, 944
ApoI RAATTY 1 cut(s) 1159
AspS9I GGNCC 6 cut(s) 4, 25, 91, 323, 1179, 1215
AsuC2I CCSGG 1 cut(s) 1121
AsuHPI GGTGA 5 cut(s) 187, 572, 911, 1064, 1154
AvaI CYCGRG 1 cut(s) 1103
AvaII GGWCC 4 cut(s) 4, 25, 1179, 1215
BaeGI GKGCMC 1 cut(s) 144
BaeI ACNNNNGTAYC 2 cut(s) 865, 898
BauI CACGAG 1 cut(s) 851
BbsI GAAGAC 2 cut(s) 842, 1274
Bbv12I GWGCWC 2 cut(s) 144, 858
BbvI GCAGC 4 cut(s) 583, 834, 931, 932
BccI CCATC 5 cut(s) 444, 592, 641, 910, 1049
BceAI ACGGC 2 cut(s) 667, 925
BciT130I CCWGG 4 cut(s) 510, 670, 871, 1183
BciVI GTATCC 1 cut(s) 544
BcnI CCSGG 1 cut(s) 1121
BcoDI GTCTC 3 cut(s) 153, 507, 546
BfaI CTAG 1 cut(s) 467
BfmI CTRYAG 2 cut(s) 249, 289
BfuAI ACCTGC 2 cut(s) 127, 725
BfuI GTATCC 1 cut(s) 544
BglII AGATCT 1 cut(s) 433
BisI GCNGC 6 cut(s) 57, 80, 572, 848, 921, 945
BlsI GCNGC 6 cut(s) 58, 81, 573, 849, 922, 946
Bme1390I CCNGG 5 cut(s) 510, 670, 871, 1121, 1183
Bme18I GGWCC 4 cut(s) 4, 25, 1179, 1215
BmeT110I CYCGRG 1 cut(s) 1103
BmgBI CACGTC 1 cut(s) 180
BmgT120I GGNCC 6 cut(s) 4, 25, 91, 323, 1179, 1215
BmiI GGNNCC 4 cut(s) 6, 614, 930, 1299
BmrFI CCNGG 5 cut(s) 510, 670, 871, 1121, 1183
BmsI GCATC 1 cut(s) 508
BoxI GACNNNNGTC 1 cut(s) 182
BpiI GAAGAC 2 cut(s) 842, 1274
BpmI CTGGAG 3 cut(s) 381, 450, 531
Bpu10I CCTNAGC 1 cut(s) 295
BpuEI CTTGAG 1 cut(s) 240
BpuMI CCSGG 1 cut(s) 1121
BsaBI GATNNNNATC 1 cut(s) 498
BsaI GGTCTC 1 cut(s) 507
BsaJI CCNNGG 5 cut(s) 104, 593, 607, 842, 869
Bsc4I CCNNNNNNNGG 2 cut(s) 622, 1188
Bse1I ACTGG 3 cut(s) 451, 481, 948
Bse3DI GCAATG 2 cut(s) 930, 932
Bse8I GATNNNNATC 1 cut(s) 498
BseBI CCWGG 4 cut(s) 510, 670, 871, 1183
BseDI CCNNGG 5 cut(s) 104, 593, 607, 842, 869
BseGI GGATG 4 cut(s) 455, 603, 652, 1259
BseJI GATNNNNATC 1 cut(s) 498
BseLI CCNNNNNNNGG 2 cut(s) 622, 1188
BseMI GCAATG 2 cut(s) 930, 932
BseMII CTCAG 3 cut(s) 309, 366, 1254
BseNI ACTGG 3 cut(s) 451, 481, 948
BseSI GKGCMC 1 cut(s) 144
BseXI GCAGC 4 cut(s) 583, 834, 931, 932
BseYI CCCAGC 1 cut(s) 132
BsgI GTGCAG 1 cut(s) 590
Bsh1236I CGCG 1 cut(s) 1002
Bsh1285I CGRYCG 1 cut(s) 77
BshFI GGCC 4 cut(s) 92, 325, 366, 592
BsiEI CGRYCG 1 cut(s) 77
BsiHKAI GWGCWC 2 cut(s) 144, 858
BsiHKCI CYCGRG 1 cut(s) 1103
BsiSI CCGG 1 cut(s) 1120
BslFI GGGAC 1 cut(s) 920
BslI CCNNNNNNNGG 2 cut(s) 622, 1188
BsmAI GTCTC 3 cut(s) 153, 507, 546
BsmFI GGGAC 1 cut(s) 920
BsnI GGCC 4 cut(s) 92, 325, 366, 592
Bso31I GGTCTC 1 cut(s) 507
BsoBI CYCGRG 1 cut(s) 1103
Bsp1286I GDGCHC 2 cut(s) 144, 858
Bsp143I GATC 6 cut(s) 15, 433, 698, 787, 811, 1219
Bsp19I CCATGG 2 cut(s) 104, 593
BspACI CCGC 9 cut(s) 42, 57, 77, 80, 172, 360, 953, 1029, 1059
BspANI GGCC 4 cut(s) 92, 325, 366, 592
BspCNI CTCAG 3 cut(s) 308, 367, 1255
BspFNI CGCG 1 cut(s) 1002
BspLI GGNNCC 4 cut(s) 6, 614, 930, 1299
BspMI ACCTGC 2 cut(s) 127, 725
BspPI GGATC 1 cut(s) 706
BspTNI GGTCTC 1 cut(s) 507
BsrDI GCAATG 2 cut(s) 930, 932
BsrI ACTGG 3 cut(s) 451, 481, 948
BssECI CCNNGG 5 cut(s) 104, 593, 607, 842, 869
BssMI GATC 6 cut(s) 15, 433, 698, 787, 811, 1219
BssSI CACGAG 1 cut(s) 851
BssT1I CCWWGG 4 cut(s) 104, 593, 607, 842
Bst2BI CACGAG 1 cut(s) 851
Bst2UI CCWGG 4 cut(s) 510, 670, 871, 1183
Bst4CI ACNGT 6 cut(s) 25, 49, 245, 316, 343, 1302
Bst6I CTCTTC 3 cut(s) 127, 132, 684
BstDEI CTNAG 4 cut(s) 295, 375, 1263, 1280
BstDSI CCRYGG 2 cut(s) 104, 593
BstEII GGTNACC 1 cut(s) 601
BstENI CCTNNNNNAGG 1 cut(s) 1186
BstF5I GGATG 4 cut(s) 455, 603, 652, 1259
BstFNI CGCG 1 cut(s) 1002
BstKTI GATC 6 cut(s) 18, 436, 701, 790, 814, 1222
BstMAI GTCTC 3 cut(s) 153, 507, 546
BstMBI GATC 6 cut(s) 15, 433, 698, 787, 811, 1219
BstMCI CGRYCG 1 cut(s) 77
BstMWI GCNNNNNNNGC 4 cut(s) 571, 815, 853, 950
BstNI CCWGG 4 cut(s) 510, 670, 871, 1183
BstNSI RCATGY 1 cut(s) 172
BstPAI GACNNNNGTC 1 cut(s) 182
BstPI GGTNACC 1 cut(s) 601
BstSCI CCNGG 5 cut(s) 508, 668, 869, 1119, 1181
BstSFI CTRYAG 2 cut(s) 249, 289
BstSLI GKGCMC 1 cut(s) 144
BstUI CGCG 1 cut(s) 1002
BstV1I GCAGC 4 cut(s) 583, 834, 931, 932
BstV2I GAAGAC 2 cut(s) 842, 1274
BstX2I RGATCY 1 cut(s) 433
BstXI CCANNNNNNTGG 1 cut(s) 1107
BstYI RGATCY 1 cut(s) 433
BsuI GTATCC 1 cut(s) 544
BsuRI GGCC 4 cut(s) 92, 325, 366, 592
BtgI CCRYGG 2 cut(s) 104, 593
BtgZI GCGATG 1 cut(s) 37
BtrI CACGTC 1 cut(s) 180
BtsCI GGATG 4 cut(s) 455, 603, 652, 1259
BtsIMutI CAGTG 2 cut(s) 488, 955
BveI ACCTGC 2 cut(s) 127, 725
Cfr13I GGNCC 6 cut(s) 4, 25, 91, 323, 1179, 1215
Csp6I GTAC 1 cut(s) 874
CspCI CAANNNNNGTGG 2 cut(s) 779, 814
CviQI GTAC 1 cut(s) 874
DdeI CTNAG 4 cut(s) 295, 375, 1263, 1280
DpnI GATC 6 cut(s) 17, 435, 700, 789, 813, 1221
DpnII GATC 6 cut(s) 15, 433, 698, 787, 811, 1219
DraI TTTAAA 1 cut(s) 642
Eam1104I CTCTTC 3 cut(s) 127, 132, 684
EarI CTCTTC 3 cut(s) 127, 132, 684
EciI GGCGGA 2 cut(s) 31, 1074
Eco130I CCWWGG 4 cut(s) 104, 593, 607, 842
Eco31I GGTCTC 1 cut(s) 507
Eco47I GGWCC 4 cut(s) 4, 25, 1179, 1215
Eco57I CTGAAG 1 cut(s) 824
Eco88I CYCGRG 1 cut(s) 1103
Eco91I GGTNACC 1 cut(s) 601
EcoNI CCTNNNNNAGG 1 cut(s) 1186
EcoO109I RGGNCCY 1 cut(s) 91
EcoO65I GGTNACC 1 cut(s) 601
EcoRI GAATTC 1 cut(s) 1159
EcoRII CCWGG 4 cut(s) 508, 668, 869, 1181
EcoT14I CCWWGG 4 cut(s) 104, 593, 607, 842
ErhI CCWWGG 4 cut(s) 104, 593, 607, 842
FalI AAGNNNNNCTT 2 cut(s) 256, 288
FaqI GGGAC 1 cut(s) 920
FauI CCCGC 1 cut(s) 165
Fnu4HI GCNGC 6 cut(s) 57, 80, 572, 848, 921, 945
FokI GGATG 4 cut(s) 462, 610, 659, 1266
Fsp4HI GCNGC 6 cut(s) 57, 80, 572, 848, 921, 945
FspBI CTAG 1 cut(s) 467
GluI GCNGC 6 cut(s) 57, 80, 572, 848, 921, 945
GsaI CCCAGC 1 cut(s) 136
GsuI CTGGAG 3 cut(s) 381, 450, 531
HaeIII GGCC 4 cut(s) 92, 325, 366, 592
HapII CCGG 1 cut(s) 1120
HincII GTYRAC 2 cut(s) 462, 826
HindII GTYRAC 2 cut(s) 462, 826
HinfI GANTC 1 cut(s) 109
HpaII CCGG 1 cut(s) 1120
HphI GGTGA 5 cut(s) 187, 572, 911, 1064, 1154
Hpy166II GTNNAC 6 cut(s) 142, 346, 462, 826, 1006, 1039
Hpy188I TCNGA 4 cut(s) 74, 787, 1021, 1219
Hpy188III TCNNGA 6 cut(s) 297, 329, 374, 388, 1228, 1251
Hpy8I GTNNAC 6 cut(s) 142, 346, 462, 826, 1006, 1039
Hpy99I CGWCG 1 cut(s) 1205
HpyAV CCTTC 4 cut(s) 216, 799, 896, 1064
HpyCH4III ACNGT 6 cut(s) 25, 49, 245, 316, 343, 1302
HpyCH4IV ACGT 2 cut(s) 69, 179
HpyCH4V TGCA 9 cut(s) 142, 155, 486, 499, 571, 581, 587, 734, 818
HpyF10VI GCNNNNNNNGC 4 cut(s) 571, 815, 853, 950
HpyF3I CTNAG 4 cut(s) 295, 375, 1263, 1280
HpySE526I ACGT 2 cut(s) 69, 179
Kzo9I GATC 6 cut(s) 15, 433, 698, 787, 811, 1219
LmnI GCTCC 3 cut(s) 208, 376, 928
Lsp1109I GCAGC 4 cut(s) 583, 834, 931, 932
LweI GCATC 1 cut(s) 508
MaeI CTAG 1 cut(s) 467
MaeII ACGT 2 cut(s) 69, 179
MaeIII GTNAC 4 cut(s) 175, 184, 601, 881
MalI GATC 6 cut(s) 17, 435, 700, 789, 813, 1221
MboI GATC 6 cut(s) 15, 433, 698, 787, 811, 1219
MboII GAAGA 6 cut(s) 114, 119, 701, 847, 1279, 1297
MflI RGATCY 1 cut(s) 433
MhlI GDGCHC 2 cut(s) 144, 858
MluCI AATT 4 cut(s) 404, 411, 664, 1159
MluI ACGCGT 1 cut(s) 1000
MlyI GAGTC 1 cut(s) 118
MmeI TCCRAC 1 cut(s) 97
MnlI CCTC 8 cut(s) 93, 104, 130, 280, 336, 356, 405, 1246
MseI TTAA 2 cut(s) 35, 641
MslI CAYNNNNRTG 6 cut(s) 173, 897, 978, 984, 1105, 1191
MspI CCGG 1 cut(s) 1120
MspR9I CCNGG 5 cut(s) 510, 670, 871, 1121, 1183
MvaI CCWGG 4 cut(s) 510, 670, 871, 1183
MvnI CGCG 1 cut(s) 1002
MwoI GCNNNNNNNGC 4 cut(s) 571, 815, 853, 950
NciI CCSGG 1 cut(s) 1121
NcoI CCATGG 2 cut(s) 104, 593
NdeII GATC 6 cut(s) 15, 433, 698, 787, 811, 1219
NlaIV GGNNCC 4 cut(s) 6, 614, 930, 1299
NmuCI GTSAC 2 cut(s) 175, 184
NspI RCATGY 1 cut(s) 172
OliI CACNNNNGTG 1 cut(s) 1105
PaqCI CACCTGC 2 cut(s) 127, 725
PflFI GACNNNGTC 1 cut(s) 457
PfoI TCCNGGA 2 cut(s) 508, 1119
PkrI GCNGC 6 cut(s) 58, 81, 573, 849, 922, 946
PleI GAGTC 1 cut(s) 117
PpsI GAGTC 1 cut(s) 117
PshAI GACNNNNGTC 1 cut(s) 182
PsiI TTATAA 1 cut(s) 276
Psp6I CCWGG 4 cut(s) 508, 668, 869, 1181
PspEI GGTNACC 1 cut(s) 601
PspFI CCCAGC 1 cut(s) 132
PspGI CCWGG 4 cut(s) 508, 668, 869, 1181
PspN4I GGNNCC 4 cut(s) 6, 614, 930, 1299
PspPI GGNCC 6 cut(s) 4, 25, 91, 323, 1179, 1215
PsuI RGATCY 1 cut(s) 433
PsyI GACNNNGTC 1 cut(s) 457
RsaI GTAC 1 cut(s) 875
RsaNI GTAC 1 cut(s) 874
RseI CAYNNNNRTG 6 cut(s) 173, 897, 978, 984, 1105, 1191
SaqAI TTAA 2 cut(s) 35, 641
SatI GCNGC 6 cut(s) 57, 80, 572, 848, 921, 945
Sau3AI GATC 6 cut(s) 15, 433, 698, 787, 811, 1219
Sau96I GGNCC 6 cut(s) 4, 25, 91, 323, 1179, 1215
SchI GAGTC 1 cut(s) 118
ScrFI CCNGG 5 cut(s) 510, 670, 871, 1121, 1183
SduI GDGCHC 2 cut(s) 144, 858
SfaNI GCATC 1 cut(s) 508
SfcI CTRYAG 2 cut(s) 249, 289
SinI GGWCC 4 cut(s) 4, 25, 1179, 1215
SmiMI CAYNNNNRTG 6 cut(s) 173, 897, 978, 984, 1105, 1191
SmlI CTYRAG 1 cut(s) 255
SmoI CTYRAG 1 cut(s) 255
Sse9I AATT 4 cut(s) 404, 411, 664, 1159
SsiI CCGC 9 cut(s) 42, 57, 77, 80, 172, 360, 953, 1029, 1059
SspMI CTAG 1 cut(s) 467
StyD4I CCNGG 5 cut(s) 508, 668, 869, 1119, 1181
StyI CCWWGG 4 cut(s) 104, 593, 607, 842
TaaI ACNGT 6 cut(s) 25, 49, 245, 316, 343, 1302
TaiI ACGT 2 cut(s) 72, 182
TaqI TCGA 1 cut(s) 112
TaqII GACCGA 1 cut(s) 843
TasI AATT 4 cut(s) 404, 411, 664, 1159
TauI GCSGC 2 cut(s) 59, 82
Tru1I TTAA 2 cut(s) 35, 641
Tru9I TTAA 2 cut(s) 35, 641
TscAI CASTG 2 cut(s) 488, 955
TseFI GTSAC 2 cut(s) 175, 184
TseI GCWGC 4 cut(s) 571, 847, 920, 944
Tsp45I GTSAC 2 cut(s) 175, 184
TspDTI ATGAA 8 cut(s) 510, 633, 702, 750, 851, 968, 1002, 1290
TspRI CASTG 2 cut(s) 488, 955
Tth111I GACNNNGTC 1 cut(s) 457
VneI GTGCAC 1 cut(s) 140
VpaK11BI GGWCC 4 cut(s) 4, 25, 1179, 1215
XagI CCTNNNNNAGG 1 cut(s) 1186
XapI RAATTY 1 cut(s) 1159
XceI RCATGY 1 cut(s) 172
XcmI CCANNNNNNNNNTGG 3 cut(s) 317, 1046, 1104
XspI CTAG 1 cut(s) 467
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.