AT3G22960

Pyruvate kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0009431
Physical Location & Seq
Forward (+)
8139185 .. 8142052
2868 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G22960.1

Sequence Viewer

Length: 1791 bp
ATGTCTCAGTCTATTCAATTCTCCACTCCTTCACACACTCCTCACCTTCTCCATCTCCCTCACTCACAATTCAACCGTCCTCTCTCCTCTATCTCCTTCCGTCGCTTCCCTCTAACAACCATCAAATACACTTCCATCAGAGCCTCCTCGTCATCATCTCCTTCACCGGATCTCGATTCATCGTCCTCATCATCATCCTCGCAAGTACTTCTCTCACCTAACGGTACTGGTGCTGTGAAGTCTGATGAGAGATCCGTTGTCGCTACGGCGGTTACGACTGATACGTCTGGGATTGAGGTTGATACTGTGACGGAAGCTGAGCTTAAGGAGAATGGATTTAGAAGTACGAGGAGGACGAAGCTGATCTGTACGATCGGACCGGCGACTTGTGGATTTGAGCAGCTTGAGGCGCTTGCTGTGGGAGGTATGAATGTGGCAAGGCTTAATATGTGTCACGGTACGCGTGATTGGCACCGCGGTGTGATTCGTAGTGTTCGGAGGCTTAATGAGGAGAAAGGCTTTGCGGTTGCTATTATGATGGATACTGAAGGTAGTGAGATTCATATGGGAGATCTTGGTGGTGAAGCTTCAGCTAAAGCAGAGGATGGTGAGGTTTGGACTTTCACTGTTAGAGCTTTTGATTCTTCTCGTCCTGAACGTACCATTAGTGTTAGCTACGATGGTTTCGCTGAAGATGTAAGAGTTGGGGATGAACTTTTGGTTGATGGTGGGATGGTGAGATTTGAAGTGATTGAGAAGATTGGTCCTGATGTTAAGTGTCTATGTACCGATCCTGGATTGTTGCTTCCTCGAGCTAACTTGACGTTTTGGAGAGATGGAAGTCTTGTACGAGAGCGTAATGCCATGCTTCCAACAATTTCTTCCAAGGACTGGTTGGATATTGATTTTGGAATTGCTGAAGGTGTGGATTTCATTGCTGTATCGTTTGTCAAGTCGGCTGAAGTCATTAATCACCTTAAAAGTTATCTTGCTGCTCGTTCCCGTGGAGGGGAAATTGGAGTGATTGCAAAGATCGAGAGTATCGATTCACTGACCAATTTGGAAGAAATTATTCTAGCATCAGATGGGGCCATGGTTGCAAGAGGAGATCTGGGAGCTCAGATACCTCTTGAGCAAGTTCCAGCAGCTCAACAGAGAATCGTCCAAGTATGCAGAGCTCTTAACAAACCCGTCATTGTCGCTTCACAGCTATTGGAGTCCATGATTGAGTACCCAACTCCAACCAGAGCAGAAGTTGCCGACGTGTCTGAAGCAGTAAGACAAAGATCAGATGCATTGATGCTCTCTGGAGAATCAGCTATGGGACAATTCCCAGACAAGGCGCTCACGGTTCTAAGGACTGTCAGTTTAAGAATCGAGAGATGGTGGAGGGAAGAGAAACGCCATGAGTCTGTACCGCTTCAAGCCATAGGCTCTTCATTTTCAGACAAAATCTCAGAAGAGATCTGTAACTCAGCTGCTAAAATGGCTAACAATCTTGGAGTGGACGCGGTTTTCGTTTACACAACGAGCGGACACATGGCATCACTGGTCTCCCGATGTCGCCCGGACTGCCCGATCTTTGCTTTCACAACCACAACCTCAGTGAGAAGACGCTTAAACCTACAATGGGGACTTATCCCATTCCGTCTCAGCTTCTCAGACGACATGGAAAGCAACTTGAACAAAACATTCTCGTTACTGAAATCAAGAGGTATGATCAAATCTGGTGACCTCGTGATCGCAGTCTCGGACATGCTGCAATCAATCCAGGTAATGAACGTCCCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000287 GO:0003006 GO:0003674 GO:0003824 GO:0004743 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006629 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009791 GO:0009987 GO:0010035 GO:0010038 GO:0010154 GO:0010431 GO:0016052 GO:0016053 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0021700 GO:0022414 GO:0030955 GO:0031420 GO:0032501 GO:0032502 GO:0032504 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042221 GO:0042866 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046686 GO:0046700 GO:0046872 GO:0046939 GO:0048316 GO:0048608 GO:0048609 GO:0048731 GO:0048856 GO:0050896 GO:0051186 GO:0051188 GO:0055086 GO:0061458 GO:0071695 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00230 ko00620 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko04922 ko04930 ko05165 ko05203 ko05230 map00010 map00230 map00620 map01100 map01110 map01120 map01130 map01200 map01230 map04922 map04930 map05165 map05203 map05230
Pfam Domains
Protein Families
PK PK_C

Protein Analysis

596

Amino Acids

65.13

Weight (kDa)

5.64

Isoelectric Point (pI)

50.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK PF00224 117 - 448 1.2e-87 Pyruvate kinase, barrel domain
PK_C PF02887 487 - 586 3.1e-26 Pyruvate kinase, alpha/beta domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 283
AccB1I GGYRCC 1 cut(s) 471
AccB7I CCANNNNNTGG 1 cut(s) 891
AccBSI CCGCTC 1 cut(s) 1533
AccII CGCG 3 cut(s) 463, 477, 1511
AciI CCGC 7 cut(s) 269, 475, 477, 524, 1418, 1511, 1533
AclWI GGATC 3 cut(s) 177, 246, 785
AcuI CTGAAG 6 cut(s) 567, 573, 711, 939, 981, 1290
AfiI CCNNNNNNNGG 5 cut(s) 891, 1008, 1009, 1339, 1630
AflII CTTAAG 1 cut(s) 323
AflIII ACRYGT 2 cut(s) 461, 1263
AgsI TTSAA 5 cut(s) 17, 73, 746, 1424, 1684
AjiI CACGTC 1 cut(s) 1264
AjnI CCWGG 2 cut(s) 793, 1770
AjuI GAANNNNNNNTTGG 2 cut(s) 865, 897
AleI CACNNNNGTG 1 cut(s) 477
Alw21I GWGCWC 2 cut(s) 1120, 1180
Alw26I GTCTC 4 cut(s) 9, 1558, 1655, 1753
AlwI GGATC 3 cut(s) 177, 246, 785
Ama87I CYCGRG 1 cut(s) 810
AoxI GGCC 1 cut(s) 1089
ApeKI GCWGC 5 cut(s) 400, 992, 1145, 1478, 1759
ArsI GACNNNNNNTTYG 2 cut(s) 1499, 1531
AseI ATTAAT 1 cut(s) 969
Asp700I GAANNNNTTC 1 cut(s) 1071
AspLEI GCGC 2 cut(s) 412, 1345
AspS9I GGNCC 3 cut(s) 377, 764, 1089
AsuC2I CCSGG 1 cut(s) 1568
AsuHPI GGTGA 8 cut(s) 35, 156, 207, 593, 620, 748, 965, 1742
AvaI CYCGRG 1 cut(s) 810
AvaII GGWCC 2 cut(s) 377, 764
BanI GGYRCC 1 cut(s) 471
BanII GRGCYC 2 cut(s) 1120, 1180
BauI CACGAG 1 cut(s) 1736
BbsI GAAGAC 1 cut(s) 1618
Bbv12I GWGCWC 2 cut(s) 1120, 1180
BbvI GCAGC 5 cut(s) 412, 979, 1157, 1465, 1746
BceAI ACGGC 1 cut(s) 282
BciT130I CCWGG 2 cut(s) 795, 1772
BciVI GTATCC 1 cut(s) 535
BclI TGATCA 1 cut(s) 1719
BcnI CCSGG 1 cut(s) 1568
BcoDI GTCTC 4 cut(s) 9, 1558, 1655, 1753
BfaI CTAG 1 cut(s) 1076
BfoI RGCGCY 2 cut(s) 413, 1346
BfrI CTTAAG 1 cut(s) 323
BfuI GTATCC 1 cut(s) 535
BglII AGATCT 3 cut(s) 571, 1108, 1464
BisI GCNGC 5 cut(s) 401, 993, 1146, 1479, 1760
BlpI GCTNAGC 1 cut(s) 318
BlsI GCNGC 5 cut(s) 402, 994, 1147, 1480, 1761
BmcAI AGTACT 1 cut(s) 207
Bme1390I CCNGG 3 cut(s) 795, 1568, 1772
Bme18I GGWCC 2 cut(s) 377, 764
BmeT110I CYCGRG 1 cut(s) 810
BmgBI CACGTC 1 cut(s) 1264
BmgT120I GGNCC 3 cut(s) 377, 764, 1089
BmiI GGNNCC 2 cut(s) 473, 1090
BmrFI CCNGG 3 cut(s) 795, 1568, 1772
BmsI GCATC 4 cut(s) 1088, 1282, 1290, 1553
BpiI GAAGAC 1 cut(s) 1618
BpmI CTGGAG 1 cut(s) 1329
Bpu1102I GCTNAGC 1 cut(s) 318
BpuEI CTTGAG 2 cut(s) 425, 1151
BpuMI CCSGG 1 cut(s) 1568
Bsa29I ATCGAT 1 cut(s) 1044
BsaI GGTCTC 1 cut(s) 1558
BsaJI CCNNGG 4 cut(s) 475, 885, 1003, 1092
BsaWI WCCGGW 1 cut(s) 166
Bsc4I CCNNNNNNNGG 5 cut(s) 891, 1008, 1009, 1339, 1630
Bse118I RCCGGY 1 cut(s) 379
Bse1I ACTGG 3 cut(s) 232, 896, 1554
Bse3DI GCAATG 1 cut(s) 933
BseBI CCWGG 2 cut(s) 795, 1772
BseCI ATCGAT 1 cut(s) 1044
BseDI CCNNGG 4 cut(s) 475, 885, 1003, 1092
BseGI GGATG 4 cut(s) 194, 610, 715, 738
BseLI CCNNNNNNNGG 5 cut(s) 891, 1008, 1009, 1339, 1630
BseMI GCAATG 1 cut(s) 933
BseMII CTCAG 8 cut(s) 20, 309, 1133, 1470, 1488, 1617, 1666, 1674
BseNI ACTGG 3 cut(s) 232, 896, 1554
BseRI GAGGAG 6 cut(s) 30, 76, 136, 364, 524, 1119
BseXI GCAGC 5 cut(s) 412, 979, 1157, 1465, 1746
Bsh1236I CGCG 3 cut(s) 463, 477, 1511
Bsh1285I CGRYCG 1 cut(s) 375
BshFI GGCC 1 cut(s) 1091
BshNI GGYRCC 1 cut(s) 471
BshVI ATCGAT 1 cut(s) 1044
BsiEI CGRYCG 1 cut(s) 375
BsiHKAI GWGCWC 2 cut(s) 1120, 1180
BsiHKCI CYCGRG 1 cut(s) 810
BsiSI CCGG 3 cut(s) 167, 380, 1568
BslFI GGGAC 3 cut(s) 1338, 1647, 1769
BslI CCNNNNNNNGG 5 cut(s) 891, 1008, 1009, 1339, 1630
BsmAI GTCTC 4 cut(s) 9, 1558, 1655, 1753
BsmBI CGTCTC 1 cut(s) 1655
BsmFI GGGAC 3 cut(s) 1338, 1647, 1769
BsnI GGCC 1 cut(s) 1091
Bso31I GGTCTC 1 cut(s) 1558
BsoBI CYCGRG 1 cut(s) 810
Bsp1286I GDGCHC 2 cut(s) 1120, 1180
Bsp1720I GCTNAGC 1 cut(s) 318
Bsp19I CCATGG 1 cut(s) 1092
BspACI CCGC 7 cut(s) 269, 475, 477, 524, 1418, 1511, 1533
BspANI GGCC 1 cut(s) 1091
BspCNI CTCAG 8 cut(s) 19, 310, 1132, 1469, 1487, 1616, 1665, 1673
BspDI ATCGAT 1 cut(s) 1044
BspFNI CGCG 3 cut(s) 463, 477, 1511
BspLI GGNNCC 2 cut(s) 473, 1090
BspPI GGATC 3 cut(s) 177, 246, 785
BspQI GCTCTTC 1 cut(s) 1441
BspT107I GGYRCC 1 cut(s) 471
BspTI CTTAAG 1 cut(s) 323
BspTNI GGTCTC 1 cut(s) 1558
BsrBI CCGCTC 1 cut(s) 1533
BsrDI GCAATG 1 cut(s) 933
BsrFI RCCGGY 1 cut(s) 379
BsrI ACTGG 3 cut(s) 232, 896, 1554
BssAI RCCGGY 1 cut(s) 379
BssECI CCNNGG 4 cut(s) 475, 885, 1003, 1092
BssSI CACGAG 1 cut(s) 1736
BssT1I CCWWGG 2 cut(s) 885, 1092
Bst2BI CACGAG 1 cut(s) 1736
Bst2UI CCWGG 2 cut(s) 795, 1772
Bst4CI ACNGT 7 cut(s) 77, 224, 307, 458, 628, 1351, 1363
Bst6I CTCTTC 3 cut(s) 1389, 1441, 1455
BstAFI CTTAAG 1 cut(s) 323
BstAPI GCANNNNNTGC 1 cut(s) 1256
BstC8I GCNNGC 1 cut(s) 414
BstDEI CTNAG 9 cut(s) 6, 318, 1119, 1355, 1456, 1474, 1603, 1652, 1660
BstDSI CCRYGG 3 cut(s) 475, 1003, 1092
BstEII GGTNACC 1 cut(s) 1730
BstF5I GGATG 4 cut(s) 194, 610, 715, 738
BstFNI CGCG 3 cut(s) 463, 477, 1511
BstH2I RGCGCY 2 cut(s) 413, 1346
BstHHI GCGC 2 cut(s) 412, 1345
BstMAI GTCTC 4 cut(s) 9, 1558, 1655, 1753
BstMCI CGRYCG 1 cut(s) 375
BstMWI GCNNNNNNNGC 6 cut(s) 409, 469, 1097, 1256, 1487, 1572
BstNI CCWGG 2 cut(s) 795, 1772
BstNSI RCATGY 1 cut(s) 1759
BstPI GGTNACC 1 cut(s) 1730
BstSCI CCNGG 3 cut(s) 793, 1566, 1770
BstUI CGCG 3 cut(s) 463, 477, 1511
BstV1I GCAGC 5 cut(s) 412, 979, 1157, 1465, 1746
BstV2I GAAGAC 1 cut(s) 1618
BstX2I RGATCY 5 cut(s) 169, 251, 571, 1108, 1464
BstYI RGATCY 5 cut(s) 169, 251, 571, 1108, 1464
Bsu15I ATCGAT 1 cut(s) 1044
BsuI GTATCC 1 cut(s) 535
BsuRI GGCC 1 cut(s) 1091
BsuTUI ATCGAT 1 cut(s) 1044
BtgI CCRYGG 3 cut(s) 475, 1003, 1092
BtrI CACGTC 1 cut(s) 1264
BtsCI GGATG 4 cut(s) 194, 610, 715, 738
BtsIMutI CAGTG 4 cut(s) 624, 1049, 1547, 1611
Cac8I GCNNGC 1 cut(s) 414
CfoI GCGC 2 cut(s) 412, 1345
Cfr10I RCCGGY 1 cut(s) 379
Cfr13I GGNCC 3 cut(s) 377, 764, 1089
Cfr42I CCGCGG 1 cut(s) 478
ClaI ATCGAT 1 cut(s) 1044
CpoI CGGWCCG 1 cut(s) 377
CseI GACGC 2 cut(s) 1517, 1623
CspI CGGWCCG 1 cut(s) 377
CviAII CATG 7 cut(s) 865, 1093, 1222, 1406, 1540, 1669, 1756
DdeI CTNAG 9 cut(s) 6, 318, 1119, 1355, 1456, 1474, 1603, 1652, 1660
DrdI GACNNNNNNGTC 1 cut(s) 283
DseDI GACNNNNNNGTC 1 cut(s) 283
Eam1104I CTCTTC 3 cut(s) 1389, 1441, 1455
EarI CTCTTC 3 cut(s) 1389, 1441, 1455
Ecl136II GAGCTC 2 cut(s) 1118, 1178
Eco130I CCWWGG 2 cut(s) 885, 1092
Eco24I GRGCYC 2 cut(s) 1120, 1180
Eco31I GGTCTC 1 cut(s) 1558
Eco47I GGWCC 2 cut(s) 377, 764
Eco53kI GAGCTC 2 cut(s) 1118, 1178
Eco57I CTGAAG 6 cut(s) 567, 573, 711, 939, 981, 1290
Eco88I CYCGRG 1 cut(s) 810
Eco91I GGTNACC 1 cut(s) 1730
EcoICRI GAGCTC 2 cut(s) 1118, 1178
EcoO65I GGTNACC 1 cut(s) 1730
EcoRII CCWGG 2 cut(s) 793, 1770
EcoT14I CCWWGG 2 cut(s) 885, 1092
EcoT22I ATGCAT 1 cut(s) 1297
EcoT38I GRGCYC 2 cut(s) 1120, 1180
ErhI CCWWGG 2 cut(s) 885, 1092
Esp3I CGTCTC 1 cut(s) 1655
FaeI CATG 7 cut(s) 868, 1096, 1225, 1409, 1543, 1672, 1759
FalI AAGNNNNNCTT 2 cut(s) 306, 338
FaqI GGGAC 3 cut(s) 1338, 1647, 1769
FatI CATG 7 cut(s) 864, 1092, 1221, 1405, 1539, 1668, 1755
FauNDI CATATG 1 cut(s) 564
FbaI TGATCA 1 cut(s) 1719
Fnu4HI GCNGC 5 cut(s) 401, 993, 1146, 1479, 1760
FokI GGATG 4 cut(s) 181, 617, 722, 745
FriOI GRGCYC 2 cut(s) 1120, 1180
Fsp4HI GCNGC 5 cut(s) 401, 993, 1146, 1479, 1760
FspBI CTAG 1 cut(s) 1076
GlaI GCGC 2 cut(s) 411, 1344
GluI GCNGC 5 cut(s) 401, 993, 1146, 1479, 1760
GsuI CTGGAG 1 cut(s) 1329
HaeII RGCGCY 2 cut(s) 413, 1346
HaeIII GGCC 1 cut(s) 1091
HapII CCGG 3 cut(s) 167, 380, 1568
HgaI GACGC 2 cut(s) 1517, 1623
HhaI GCGC 2 cut(s) 412, 1345
Hin1II CATG 7 cut(s) 868, 1096, 1225, 1409, 1543, 1672, 1759
Hin6I GCGC 2 cut(s) 410, 1343
HinP1I GCGC 2 cut(s) 410, 1343
HindIII AAGCTT 1 cut(s) 585
HpaII CCGG 3 cut(s) 167, 380, 1568
HphI GGTGA 8 cut(s) 35, 156, 207, 593, 620, 748, 965, 1742
Hpy166II GTNNAC 2 cut(s) 1507, 1522
Hpy8I GTNNAC 2 cut(s) 1507, 1522
Hpy99I CGWCG 2 cut(s) 105, 1265
HpyAV CCTTC 6 cut(s) 39, 56, 106, 171, 542, 914
HpyCH4III ACNGT 7 cut(s) 77, 224, 307, 458, 628, 1351, 1363
HpyCH4IV ACGT 5 cut(s) 284, 658, 824, 1263, 1782
HpyCH4V TGCA 5 cut(s) 1028, 1100, 1173, 1295, 1762
HpyF10VI GCNNNNNNNGC 6 cut(s) 409, 469, 1097, 1256, 1487, 1572
HpyF3I CTNAG 9 cut(s) 6, 318, 1119, 1355, 1456, 1474, 1603, 1652, 1660
HpySE526I ACGT 5 cut(s) 284, 658, 824, 1263, 1782
Hsp92II CATG 7 cut(s) 868, 1096, 1225, 1409, 1543, 1672, 1759
HspAI GCGC 2 cut(s) 410, 1343
Ksp22I TGATCA 1 cut(s) 1719
KspI CCGCGG 1 cut(s) 478
LguI GCTCTTC 1 cut(s) 1441
LmnI GCTCC 1 cut(s) 1115
Lsp1109I GCAGC 5 cut(s) 412, 979, 1157, 1465, 1746
LweI GCATC 4 cut(s) 1088, 1282, 1290, 1553
MaeI CTAG 1 cut(s) 1076
MaeII ACGT 5 cut(s) 284, 658, 824, 1263, 1782
MaeIII GTNAC 6 cut(s) 271, 307, 452, 1469, 1698, 1730
MbiI CCGCTC 1 cut(s) 1533
MboII GAAGA 9 cut(s) 636, 704, 769, 873, 1076, 1406, 1428, 1472, 1623
MflI RGATCY 5 cut(s) 169, 251, 571, 1108, 1464
MhlI GDGCHC 2 cut(s) 1120, 1180
MluCI AATT 8 cut(s) 17, 68, 876, 912, 1014, 1057, 1068, 1328
MluI ACGCGT 1 cut(s) 461
MlyI GAGTC 2 cut(s) 1226, 1418
MmeI TCCRAC 3 cut(s) 876, 896, 1265
Mph1103I ATGCAT 1 cut(s) 1297
MroXI GAANNNNTTC 1 cut(s) 1071
MseI TTAA 9 cut(s) 324, 444, 504, 774, 969, 978, 1182, 1370, 1619
MslI CAYNNNNRTG 1 cut(s) 477
MspA1I CMGCKG 2 cut(s) 477, 1478
MspCI CTTAAG 1 cut(s) 323
MspI CCGG 3 cut(s) 167, 380, 1568
MspR9I CCNGG 3 cut(s) 795, 1568, 1772
MvaI CCWGG 2 cut(s) 795, 1772
MvnI CGCG 3 cut(s) 463, 477, 1511
MwoI GCNNNNNNNGC 6 cut(s) 409, 469, 1097, 1256, 1487, 1572
NciI CCSGG 1 cut(s) 1568
NcoI CCATGG 1 cut(s) 1092
NdeI CATATG 1 cut(s) 564
NlaIII CATG 7 cut(s) 868, 1096, 1225, 1409, 1543, 1672, 1759
NlaIV GGNNCC 2 cut(s) 473, 1090
NmuCI GTSAC 3 cut(s) 307, 452, 1730
NsiI ATGCAT 1 cut(s) 1297
NspI RCATGY 1 cut(s) 1759
OliI CACNNNNGTG 1 cut(s) 477
PaeR7I CTCGAG 1 cut(s) 810
PciSI GCTCTTC 1 cut(s) 1441
PcsI WCGNNNNNNNCGW 6 cut(s) 272, 281, 353, 655, 1041, 1515
PdmI GAANNNNTTC 1 cut(s) 1071
PfeI GAWTC 8 cut(s) 176, 484, 559, 641, 1046, 1158, 1313, 1374
PflMI CCANNNNNTGG 1 cut(s) 891
PfoI TCCNGGA 1 cut(s) 793
PkrI GCNGC 5 cut(s) 402, 994, 1147, 1480, 1761
Ple19I CGATCG 1 cut(s) 375
PleI GAGTC 2 cut(s) 1225, 1417
PpsI GAGTC 2 cut(s) 1225, 1417
PshBI ATTAAT 1 cut(s) 969
Psp124BI GAGCTC 2 cut(s) 1120, 1180
Psp6I CCWGG 2 cut(s) 793, 1770
PspEI GGTNACC 1 cut(s) 1730
PspGI CCWGG 2 cut(s) 793, 1770
PspN4I GGNNCC 2 cut(s) 473, 1090
PspPI GGNCC 3 cut(s) 377, 764, 1089
PspXI VCTCGAGB 1 cut(s) 810
PsuI RGATCY 5 cut(s) 169, 251, 571, 1108, 1464
PvuI CGATCG 1 cut(s) 375
PvuII CAGCTG 1 cut(s) 1478
RseI CAYNNNNRTG 1 cut(s) 477
Rsr2I CGGWCCG 1 cut(s) 377
RsrII CGGWCCG 1 cut(s) 377
SacI GAGCTC 2 cut(s) 1120, 1180
SacII CCGCGG 1 cut(s) 478
SapI GCTCTTC 1 cut(s) 1441
SaqAI TTAA 9 cut(s) 324, 444, 504, 774, 969, 978, 1182, 1370, 1619
SatI GCNGC 5 cut(s) 401, 993, 1146, 1479, 1760
Sau96I GGNCC 3 cut(s) 377, 764, 1089
ScaI AGTACT 1 cut(s) 207
SchI GAGTC 2 cut(s) 1226, 1418
ScrFI CCNGG 3 cut(s) 795, 1568, 1772
SduI GDGCHC 2 cut(s) 1120, 1180
SfaNI GCATC 4 cut(s) 1088, 1282, 1290, 1553
Sfr274I CTCGAG 1 cut(s) 810
Sfr303I CCGCGG 1 cut(s) 478
SgrBI CCGCGG 1 cut(s) 478
SinI GGWCC 2 cut(s) 377, 764
SlaI CTCGAG 1 cut(s) 810
SmiMI CAYNNNNRTG 1 cut(s) 477
SmlI CTYRAG 4 cut(s) 323, 404, 810, 1130
SmoI CTYRAG 4 cut(s) 323, 404, 810, 1130
Sse9I AATT 8 cut(s) 17, 68, 876, 912, 1014, 1057, 1068, 1328
SsiI CCGC 7 cut(s) 269, 475, 477, 524, 1418, 1511, 1533
SspMI CTAG 1 cut(s) 1076
SstI GAGCTC 2 cut(s) 1120, 1180
StyD4I CCNGG 3 cut(s) 793, 1566, 1770
StyI CCWWGG 2 cut(s) 885, 1092
TaaI ACNGT 7 cut(s) 77, 224, 307, 458, 628, 1351, 1363
TaiI ACGT 5 cut(s) 287, 661, 827, 1266, 1785
TaqI TCGA 5 cut(s) 174, 811, 1035, 1044, 1377
TasI AATT 8 cut(s) 17, 68, 876, 912, 1014, 1057, 1068, 1328
TatI WGTACW 1 cut(s) 205
TfiI GAWTC 8 cut(s) 176, 484, 559, 641, 1046, 1158, 1313, 1374
Tru1I TTAA 9 cut(s) 324, 444, 504, 774, 969, 978, 1182, 1370, 1619
Tru9I TTAA 9 cut(s) 324, 444, 504, 774, 969, 978, 1182, 1370, 1619
TscAI CASTG 4 cut(s) 631, 1056, 1554, 1611
TseFI GTSAC 3 cut(s) 307, 452, 1730
TseI GCWGC 5 cut(s) 400, 992, 1145, 1478, 1759
Tsp45I GTSAC 3 cut(s) 307, 452, 1730
TspDTI ATGAA 6 cut(s) 168, 443, 551, 726, 922, 1428
TspGWI ACGGA 4 cut(s) 89, 244, 326, 1637
TspRI CASTG 4 cut(s) 631, 1056, 1554, 1611
Van91I CCANNNNNTGG 1 cut(s) 891
Vha464I CTTAAG 1 cut(s) 323
VpaK11BI GGWCC 2 cut(s) 377, 764
VspI ATTAAT 1 cut(s) 969
XceI RCATGY 1 cut(s) 1759
XcmI CCANNNNNNNNNTGG 1 cut(s) 892
XhoI CTCGAG 1 cut(s) 810
XmnI GAANNNNTTC 1 cut(s) 1071
XspI CTAG 1 cut(s) 1076
ZrmI AGTACT 1 cut(s) 207
Zsp2I ATGCAT 1 cut(s) 1297
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.