Rorug03G0320700

Belongs to the pyruvate kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0009431
Physical Location & Seq
Forward (+)
34669009 .. 34670829
1821 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0320700.1

Sequence Viewer

Length: 942 bp
ATGTTTCGCACTGTGGTATCGGGGTTCCGCCACCGTTGGGCACAAGGAAATGAAAAGTTAAACAACAAGGTTGGTAGGGTGCTGTGCTATTCTTCCGATGCCCAACCAAATGGCACAAAGACTTTGAGGGGTTGGAAGAAAGACTGTGAACCGACTCATAAAATCACTGATAAGTATGGGAAAGAAGTTCTTACATATGATGGGTTAACAGCACCAGGGATACTAGTACCCCTTGATTATCGAAAGCTAGTCAGTAGTCTACTTTTCAACATGATAGAGCTAGATTGTGAGAATCAAAACTTTGACCTTAGCTTGGATTCAATCAGCGTATTTAGAGGCTTGCCTTGGAAATTGAATGACATGATCGACCTAATTCTTTCTGATCGTGATATTGACTCGGCCCTTACACATGTGGCGACGCATTCTTCTATTAAGAGAAACATCAATCGCCGATATCTCTTTTCAAGGATCAGTGAGCTAGATGATTCTAAAGTGAGGGTGCATAACTTAACACTGAACAGATGGTGGCCATTTAAGAGAGATACTTATAGTAATGTGGTGTGGTTGGTTACGGTAGAGAGAAACAAAGGTTCAAATGCATTGGATCATCTTATATGGACCCACAATCTAGGAGTGTTTACACCACTTGAAATTAGTGTTTATCTTGCACCCATAGATCTTAAAAGGATACACAAGCCATCCGAGTTTCTGCGGATCTCAACACATGTTATTGTTCATCAACTTCCTCAGCAACTCTATAAAATGAAATTAGGGAGTACTGAAATGAAGCTTCATGAAGTTGATACGGTTTTGAGCTTAGCTTGGCGCCAGCATTTGGTGTTTACCAACAATTTTGCACTTGCATTTAAGAAACAAACATGCTCAGCTTTTGTTACGTCCAGCTCTAGTGGTCATGTTCCATGGACGCACGTAAATCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000287 GO:0003006 GO:0003674 GO:0003824 GO:0004743 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006629 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009791 GO:0009987 GO:0010035 GO:0010038 GO:0010154 GO:0010431 GO:0016052 GO:0016053 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0021700 GO:0022414 GO:0030955 GO:0031420 GO:0032501 GO:0032502 GO:0032504 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042221 GO:0042866 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046686 GO:0046700 GO:0046872 GO:0046939 GO:0048316 GO:0048608 GO:0048609 GO:0048731 GO:0048856 GO:0050896 GO:0051186 GO:0051188 GO:0055086 GO:0061458 GO:0071695 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00230 ko00620 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko04922 ko04930 ko05165 ko05203 ko05230 map00010 map00230 map00620 map01100 map01110 map01120 map01130 map01200 map01230 map04922 map04930 map05165 map05203 map05230
Pfam Domains
Protein Families
PK PK_C

Protein Analysis

313

Amino Acids

36.17

Weight (kDa)

9.5

Isoelectric Point (pI)

36.61

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 825
AccB7I CCANNNNNTGG 1 cut(s) 835
AccI GTMKAC 1 cut(s) 259
AciI CCGC 2 cut(s) 28, 712
AclWI GGATC 3 cut(s) 476, 612, 722
AcoI YGGCCR 1 cut(s) 527
AcyI GRCGYC 1 cut(s) 826
AfaI GTAC 2 cut(s) 228, 778
AfiI CCNNNNNNNGG 3 cut(s) 37, 313, 835
AflIII ACRYGT 2 cut(s) 409, 724
AgsI TTSAA 6 cut(s) 268, 321, 355, 465, 594, 650
AhlI ACTAGT 1 cut(s) 223
AjnI CCWGG 1 cut(s) 214
AluBI AGCT 9 cut(s) 247, 280, 312, 478, 790, 816, 821, 887, 903
AluI AGCT 9 cut(s) 247, 280, 312, 478, 790, 816, 821, 887, 903
AlwI GGATC 3 cut(s) 476, 612, 722
AoxI GGCC 2 cut(s) 399, 527
AspLEI GCGC 1 cut(s) 828
AspS9I GGNCC 2 cut(s) 400, 618
AvaII GGWCC 1 cut(s) 618
BaeGI GKGCMC 1 cut(s) 43
BaeI ACNNNNGTAYC 1 cut(s) 33
BalI TGGCCA 1 cut(s) 529
BanI GGYRCC 1 cut(s) 825
BbvCI CCTCAGC 1 cut(s) 747
BccI CCATC 3 cut(s) 194, 516, 706
BcgI CGANNNNNNTGC 1 cut(s) 917
BciT130I CCWGG 1 cut(s) 216
BciVI GTATCC 2 cut(s) 213, 681
BcuI ACTAGT 1 cut(s) 223
BfaI CTAG 6 cut(s) 224, 248, 281, 479, 629, 906
BfoI RGCGCY 1 cut(s) 829
BfuI GTATCC 2 cut(s) 213, 681
BglII AGATCT 1 cut(s) 676
BlpI GCTNAGC 2 cut(s) 817, 883
BmcAI AGTACT 1 cut(s) 778
Bme1390I CCNGG 1 cut(s) 216
Bme18I GGWCC 1 cut(s) 618
BmgT120I GGNCC 2 cut(s) 400, 618
BmiI GGNNCC 3 cut(s) 26, 620, 827
BmrFI CCNGG 1 cut(s) 216
BmsI GCATC 1 cut(s) 88
Bpu10I CCTNAGC 2 cut(s) 308, 747
Bpu1102I GCTNAGC 2 cut(s) 817, 883
BsaAI YACGTR 1 cut(s) 931
BsaHI GRCGYC 1 cut(s) 826
BsaJI CCNNGG 3 cut(s) 215, 344, 920
Bsc4I CCNNNNNNNGG 3 cut(s) 37, 313, 835
BseBI CCWGG 1 cut(s) 216
BseDI CCNNGG 3 cut(s) 215, 344, 920
BseGI GGATG 1 cut(s) 698
BseLI CCNNNNNNNGG 3 cut(s) 37, 313, 835
BseMII CTCAG 2 cut(s) 761, 897
BseSI GKGCMC 1 cut(s) 43
BshFI GGCC 2 cut(s) 401, 529
BshNI GGYRCC 1 cut(s) 825
BslI CCNNNNNNNGG 3 cut(s) 37, 313, 835
BsmI GAATGC 1 cut(s) 421
BsnI GGCC 2 cut(s) 401, 529
Bsp1286I GDGCHC 1 cut(s) 43
Bsp143I GATC 6 cut(s) 363, 382, 468, 604, 676, 714
Bsp1720I GCTNAGC 2 cut(s) 817, 883
Bsp19I CCATGG 1 cut(s) 920
BspACI CCGC 2 cut(s) 28, 712
BspANI GGCC 2 cut(s) 401, 529
BspCNI CTCAG 2 cut(s) 760, 896
BspHI TCATGA 1 cut(s) 793
BspLI GGNNCC 3 cut(s) 26, 620, 827
BspPI GGATC 3 cut(s) 476, 612, 722
BspT107I GGYRCC 1 cut(s) 825
BssECI CCNNGG 3 cut(s) 215, 344, 920
BssMI GATC 6 cut(s) 363, 382, 468, 604, 676, 714
BssNI GRCGYC 1 cut(s) 826
BssT1I CCWWGG 2 cut(s) 344, 920
Bst2UI CCWGG 1 cut(s) 216
Bst4CI ACNGT 5 cut(s) 13, 35, 146, 574, 808
BstACI GRCGYC 1 cut(s) 826
BstBAI YACGTR 1 cut(s) 931
BstC8I GCNNGC 2 cut(s) 341, 830
BstDEI CTNAG 4 cut(s) 308, 747, 817, 883
BstDSI CCRYGG 1 cut(s) 920
BstF5I GGATG 1 cut(s) 698
BstH2I RGCGCY 1 cut(s) 829
BstHHI GCGC 1 cut(s) 828
BstKTI GATC 6 cut(s) 366, 385, 471, 607, 679, 717
BstMBI GATC 6 cut(s) 363, 382, 468, 604, 676, 714
BstNI CCWGG 1 cut(s) 216
BstNSI RCATGY 3 cut(s) 413, 728, 882
BstSCI CCNGG 1 cut(s) 214
BstSLI GKGCMC 1 cut(s) 43
BstX2I RGATCY 2 cut(s) 676, 714
BstXI CCANNNNNNTGG 1 cut(s) 110
BstYI RGATCY 2 cut(s) 676, 714
BsuI GTATCC 2 cut(s) 213, 681
BsuRI GGCC 2 cut(s) 401, 529
BtgI CCRYGG 1 cut(s) 920
BtsCI GGATG 1 cut(s) 698
BtsIMutI CAGTG 4 cut(s) 9, 165, 478, 512
Cac8I GCNNGC 2 cut(s) 341, 830
CciI TCATGA 1 cut(s) 793
CfoI GCGC 1 cut(s) 828
Cfr13I GGNCC 2 cut(s) 400, 618
CseI GACGC 2 cut(s) 427, 934
Csp6I GTAC 2 cut(s) 227, 777
CviAII CATG 8 cut(s) 271, 361, 410, 725, 794, 879, 914, 921
CviQI GTAC 2 cut(s) 227, 777
DdeI CTNAG 4 cut(s) 308, 747, 817, 883
DinI GGCGCC 1 cut(s) 827
DpnI GATC 6 cut(s) 365, 384, 470, 606, 678, 716
DpnII GATC 6 cut(s) 363, 382, 468, 604, 676, 714
EaeI YGGCCR 1 cut(s) 527
EciI GGCGGA 1 cut(s) 17
Eco130I CCWWGG 2 cut(s) 344, 920
Eco32I GATATC 1 cut(s) 455
Eco47I GGWCC 1 cut(s) 618
EcoRII CCWGG 1 cut(s) 214
EcoRV GATATC 1 cut(s) 455
EcoT14I CCWWGG 2 cut(s) 344, 920
EcoT22I ATGCAT 1 cut(s) 601
EgeI GGCGCC 1 cut(s) 827
EheI GGCGCC 1 cut(s) 827
ErhI CCWWGG 2 cut(s) 344, 920
FaeI CATG 8 cut(s) 274, 364, 413, 728, 797, 882, 917, 924
FalI AAGNNNNNCTT 2 cut(s) 174, 206
FatI CATG 8 cut(s) 270, 360, 409, 724, 793, 878, 913, 920
FauNDI CATATG 1 cut(s) 196
FblI GTMKAC 1 cut(s) 259
FokI GGATG 1 cut(s) 685
FspBI CTAG 6 cut(s) 224, 248, 281, 479, 629, 906
GlaI GCGC 1 cut(s) 827
HaeII RGCGCY 1 cut(s) 829
HaeIII GGCC 2 cut(s) 401, 529
HgaI GACGC 2 cut(s) 427, 934
HhaI GCGC 1 cut(s) 828
Hin1I GRCGYC 1 cut(s) 826
Hin1II CATG 8 cut(s) 274, 364, 413, 728, 797, 882, 917, 924
Hin6I GCGC 1 cut(s) 826
HinP1I GCGC 1 cut(s) 826
HincII GTYRAC 1 cut(s) 207
HindII GTYRAC 1 cut(s) 207
HindIII AAGCTT 1 cut(s) 788
HinfI GANTC 5 cut(s) 154, 292, 317, 395, 485
HpaI GTTAAC 1 cut(s) 207
Hpy166II GTNNAC 5 cut(s) 149, 207, 260, 639, 843
Hpy188I TCNGA 3 cut(s) 97, 382, 703
Hpy188III TCNNGA 2 cut(s) 386, 794
Hpy8I GTNNAC 5 cut(s) 149, 207, 260, 639, 843
Hpy99I CGWCG 1 cut(s) 421
HpyCH4III ACNGT 5 cut(s) 13, 35, 146, 574, 808
HpyCH4IV ACGT 2 cut(s) 896, 930
HpyCH4V TGCA 5 cut(s) 502, 599, 668, 857, 863
HpyF3I CTNAG 4 cut(s) 308, 747, 817, 883
HpySE526I ACGT 2 cut(s) 896, 930
Hsp92I GRCGYC 1 cut(s) 826
Hsp92II CATG 8 cut(s) 274, 364, 413, 728, 797, 882, 917, 924
HspAI GCGC 1 cut(s) 826
KasI GGCGCC 1 cut(s) 825
KspAI GTTAAC 1 cut(s) 207
Kzo9I GATC 6 cut(s) 363, 382, 468, 604, 676, 714
LpnPI CCDG 4 cut(s) 201, 228, 842, 913
LweI GCATC 1 cut(s) 88
MaeI CTAG 6 cut(s) 224, 248, 281, 479, 629, 906
MaeII ACGT 2 cut(s) 896, 930
MaeIII GTNAC 2 cut(s) 568, 892
MalI GATC 6 cut(s) 365, 384, 470, 606, 678, 716
MboI GATC 6 cut(s) 363, 382, 468, 604, 676, 714
MboII GAAGA 3 cut(s) 84, 148, 417
MflI RGATCY 2 cut(s) 676, 714
MhlI GDGCHC 1 cut(s) 43
MlsI TGGCCA 1 cut(s) 529
MluCI AATT 5 cut(s) 350, 372, 651, 767, 850
MluNI TGGCCA 1 cut(s) 529
Mly113I GGCGCC 1 cut(s) 826
MlyI GAGTC 2 cut(s) 148, 389
MmeI TCCRAC 1 cut(s) 113
MnlI CCTC 4 cut(s) 120, 329, 489, 756
Mox20I TGGCCA 1 cut(s) 529
Mph1103I ATGCAT 1 cut(s) 601
MscI TGGCCA 1 cut(s) 529
MseI TTAA 7 cut(s) 59, 206, 432, 509, 534, 681, 867
Msp20I TGGCCA 1 cut(s) 529
MspR9I CCNGG 1 cut(s) 216
Mva1269I GAATGC 1 cut(s) 421
MvaI CCWGG 1 cut(s) 216
NarI GGCGCC 1 cut(s) 826
NcoI CCATGG 1 cut(s) 920
NdeI CATATG 1 cut(s) 196
NdeII GATC 6 cut(s) 363, 382, 468, 604, 676, 714
NlaIII CATG 8 cut(s) 274, 364, 413, 728, 797, 882, 917, 924
NlaIV GGNNCC 3 cut(s) 26, 620, 827
NmeAIII GCCGAG 1 cut(s) 377
NsiI ATGCAT 1 cut(s) 601
NspI RCATGY 3 cut(s) 413, 728, 882
PagI TCATGA 1 cut(s) 793
PciI ACATGT 2 cut(s) 409, 724
PctI GAATGC 1 cut(s) 421
PfeI GAWTC 3 cut(s) 292, 317, 485
PflMI CCANNNNNTGG 1 cut(s) 835
PleI GAGTC 2 cut(s) 148, 389
PluTI GGCGCC 1 cut(s) 829
PpsI GAGTC 2 cut(s) 148, 389
Ppu21I YACGTR 1 cut(s) 931
PscI ACATGT 2 cut(s) 409, 724
Psp6I CCWGG 1 cut(s) 214
PspGI CCWGG 1 cut(s) 214
PspN4I GGNNCC 3 cut(s) 26, 620, 827
PspPI GGNCC 2 cut(s) 400, 618
PsuI RGATCY 2 cut(s) 676, 714
RsaI GTAC 2 cut(s) 228, 778
RsaNI GTAC 2 cut(s) 227, 777
SaqAI TTAA 7 cut(s) 59, 206, 432, 509, 534, 681, 867
Sau3AI GATC 6 cut(s) 363, 382, 468, 604, 676, 714
Sau96I GGNCC 2 cut(s) 400, 618
ScaI AGTACT 1 cut(s) 778
SchI GAGTC 2 cut(s) 148, 389
ScrFI CCNGG 1 cut(s) 216
SduI GDGCHC 1 cut(s) 43
SfaNI GCATC 1 cut(s) 88
SfoI GGCGCC 1 cut(s) 827
SinI GGWCC 1 cut(s) 618
SpeI ACTAGT 1 cut(s) 223
Sse9I AATT 5 cut(s) 350, 372, 651, 767, 850
SsiI CCGC 2 cut(s) 28, 712
SspDI GGCGCC 1 cut(s) 825
SspMI CTAG 6 cut(s) 224, 248, 281, 479, 629, 906
StyD4I CCNGG 1 cut(s) 214
StyI CCWWGG 2 cut(s) 344, 920
TaaI ACNGT 5 cut(s) 13, 35, 146, 574, 808
TaiI ACGT 2 cut(s) 899, 933
TaqI TCGA 2 cut(s) 241, 366
TasI AATT 5 cut(s) 350, 372, 651, 767, 850
TatI WGTACW 1 cut(s) 776
TfiI GAWTC 3 cut(s) 292, 317, 485
Tru1I TTAA 7 cut(s) 59, 206, 432, 509, 534, 681, 867
Tru9I TTAA 7 cut(s) 59, 206, 432, 509, 534, 681, 867
TscAI CASTG 4 cut(s) 16, 172, 478, 519
TspDTI ATGAA 6 cut(s) 66, 725, 779, 782, 800, 810
TspRI CASTG 4 cut(s) 16, 172, 478, 519
Van91I CCANNNNNTGG 1 cut(s) 835
VpaK11BI GGWCC 1 cut(s) 618
XceI RCATGY 3 cut(s) 413, 728, 882
XmiI GTMKAC 1 cut(s) 259
XspI CTAG 6 cut(s) 224, 248, 281, 479, 629, 906
ZrmI AGTACT 1 cut(s) 778
Zsp2I ATGCAT 1 cut(s) 601
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.