RLG00000010018

Belongs to the pyruvate kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0009431
Physical Location & Seq
Reverse (-)
61799670 .. 61804418
4749 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000010018

Sequence Viewer

Length: 1323 bp
ATGAACGTCGCCAGGCTCAACATGTGCCACGGCACGCGCGACTGGCACCGCGCCGTGATTGAGCGCGTGAGGAAGCTCAACGAGGACAAGGGCTACGCCGTCGCGATTATGATGGATACCGAAGGGAGTGAGATTCACATGGGCGATCTCGGCACTTCCTCCGTCAAAGCCGAGGATGGTGAGATATGGACTTTCAGTGTCAGAGCGTTCGGTTCTACTCTTCCGGAACGCACGATCAATGTGAATTACGAGGGCTTCGCCGAAGATGTGAAAGTGGGCGATGACCTACTTGTGGATGGTGGAATGGTGAGGTTTGAGGTGGTTGAGAAGCTTGGCCCGGATGTCAAGTGTAAATGTACTGATCCAGGACTGTTGCTGCCGCGGGCTAACTTGACTTTCTGGAGGGATGGGAGTTTAGTGCGAGAACGAAATGCCATGCTTCCCACAATTTCTTCCAAGGGTGTTGATTTCATCGCTGTATCTTTTGTCAAGTCTGCTGAAGTGATCAATCATCTTAAAAGCTATATTGCAGCACGGTCTAGCGATATTTCTGTTGTGGCAAAGATAGAGAGTATTGACTCTCTAAAGAACTTGGAAGAGATCATCCTTGCATCAGATGGAGCAATGGTAGCAAGAGGTGACCTAGGTGCTCAGATACCATTGGAACAGGTCCCATCAGCCCAGCAAAAGATTGTTCAAGTATGTCGGCAGCTAAATAAGCCAGTCATCGTTGCTTCTCAACTACTCGAATCTATGATCGAGTATCCTACACCCACTAGAGCTGAAGTGGCCGATGTTTCTGAAGCCGTGAGGCAGCGAGCTGATGCTCTGATGCTTTCTGGTGAGTCAGCTATGGGCCAGTTCCCAGATAAGTCATTAGCTGTTCTCAGAAGTGTCAGTTTGAGGATCGAGAGGTGGTGGAGGGAAGAGAAACGTCATGAACCTATGTTACTCCCAGCTATTGGATCATCATTTTCAGACAGTATATCGGAGGAGCTTTGTATTTCTGCTGCCAAGATGGCAAATAATTTAGAGGTGGATGCTCTTTTCATCTTTACAAAGACAGGCCACATGGCATCTCTCCTGTCGCGCTGCCGGCCTGACTGCCCAATCTTTGCTTTTACCAACACAACATCTGTGCGGAGACGTCTGAACCTACAGTGGGGGCTGATTCCATTTCGTGTTGGTTTCTCTGATGATATGGAAAGCAACCTTCACAAGACCTTCTCACTGCTGAAGGCCAGGAATCTGATCAAATCAGGTGACCTTGTCATTGCTGTCTCAGACATATTGCAGTCCATTCAAGTTATGAATGTACCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000287 GO:0003006 GO:0003674 GO:0003824 GO:0004743 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006629 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009791 GO:0009987 GO:0010035 GO:0010038 GO:0010154 GO:0010431 GO:0016052 GO:0016053 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0021700 GO:0022414 GO:0030955 GO:0031420 GO:0032501 GO:0032502 GO:0032504 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042221 GO:0042866 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046686 GO:0046700 GO:0046872 GO:0046939 GO:0048316 GO:0048608 GO:0048609 GO:0048731 GO:0048856 GO:0050896 GO:0051186 GO:0051188 GO:0055086 GO:0061458 GO:0071695 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00230 ko00620 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko04922 ko04930 ko05165 ko05203 ko05230 map00010 map00230 map00620 map01100 map01110 map01120 map01130 map01200 map01230 map04922 map04930 map05165 map05203 map05230
Pfam Domains
Protein Families
PK PK_C

Protein Analysis

441

Amino Acids

48.7

Weight (kDa)

5.79

Isoelectric Point (pI)

42.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK PF00224 1 - 292 7.5e-74 Pyruvate kinase, barrel domain
PK_C PF02887 331 - 430 3e-23 Pyruvate kinase, alpha/beta domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1150
AccB1I GGYRCC 1 cut(s) 45
AccB7I CCANNNNNTGG 1 cut(s) 962
AccII CGCG 7 cut(s) 37, 39, 51, 66, 104, 382, 1090
AccIII TCCGGA 1 cut(s) 223
AciI CCGC 4 cut(s) 49, 380, 382, 1141
AclWI GGATC 3 cut(s) 356, 914, 973
AcoI YGGCCR 1 cut(s) 789
AcuI CTGAAG 4 cut(s) 519, 804, 822, 1256
AcyI GRCGYC 1 cut(s) 1147
AfaI GTAC 2 cut(s) 358, 1317
AfiI CCNNNNNNNGG 3 cut(s) 292, 962, 1162
AflIII ACRYGT 1 cut(s) 21
AgsI TTSAA 2 cut(s) 698, 1304
AjnI CCWGG 3 cut(s) 11, 364, 1241
Alw21I GWGCWC 1 cut(s) 652
Alw26I GTCTC 2 cut(s) 1138, 1285
AlwI GGATC 3 cut(s) 356, 914, 973
Aor13HI TCCGGA 1 cut(s) 223
AoxI GGCC 6 cut(s) 334, 789, 856, 1066, 1097, 1239
ApeKI GCWGC 6 cut(s) 376, 530, 709, 814, 1010, 1092
AspA2I CCTAGG 1 cut(s) 643
AspLEI GCGC 4 cut(s) 39, 53, 66, 1092
AspS9I GGNCC 3 cut(s) 335, 670, 856
AsuC2I CCSGG 1 cut(s) 338
AsuHPI GGTGA 5 cut(s) 191, 319, 650, 854, 1274
AvaII GGWCC 1 cut(s) 670
AvrII CCTAGG 1 cut(s) 643
BanI GGYRCC 1 cut(s) 45
Bbv12I GWGCWC 1 cut(s) 652
BbvI GCAGC 6 cut(s) 363, 542, 721, 826, 997, 1079
BccI CCATC 7 cut(s) 106, 170, 290, 401, 611, 682, 1012
BceAI ACGGC 4 cut(s) 38, 46, 83, 791
BciT130I CCWGG 3 cut(s) 13, 366, 1243
BciVI GTATCC 2 cut(s) 109, 774
BclI TGATCA 2 cut(s) 504, 1251
BcnI CCSGG 1 cut(s) 338
BcoDI GTCTC 2 cut(s) 1138, 1285
BfaI CTAG 3 cut(s) 540, 644, 777
BfmI CTRYAG 1 cut(s) 1157
BfuI GTATCC 2 cut(s) 109, 774
BglI GCCNNNNNGGC 1 cut(s) 1019
BisI GCNGC 7 cut(s) 377, 380, 531, 710, 815, 1011, 1093
BlnI CCTAGG 1 cut(s) 643
BlsI GCNGC 7 cut(s) 378, 381, 532, 711, 816, 1012, 1094
Bme1390I CCNGG 4 cut(s) 13, 338, 366, 1243
Bme18I GGWCC 1 cut(s) 670
BmgT120I GGNCC 3 cut(s) 335, 670, 856
BmiI GGNNCC 2 cut(s) 47, 672
BmrFI CCNGG 4 cut(s) 13, 338, 366, 1243
BmsI GCATC 5 cut(s) 620, 814, 822, 1030, 1085
BpmI CTGGAG 1 cut(s) 421
BpuMI CCSGG 1 cut(s) 338
BsaHI GRCGYC 1 cut(s) 1147
BsaJI CCNNGG 5 cut(s) 28, 171, 380, 456, 643
BsaWI WCCGGW 1 cut(s) 223
BsaXI ACNNNNNCTCC 2 cut(s) 612, 642
Bsc4I CCNNNNNNNGG 3 cut(s) 292, 962, 1162
Bse118I RCCGGY 1 cut(s) 1095
Bse1I ACTGG 3 cut(s) 47, 722, 859
Bse3DI GCAATG 2 cut(s) 630, 1272
BseAI TCCGGA 1 cut(s) 223
BseBI CCWGG 3 cut(s) 13, 366, 1243
BseDI CCNNGG 5 cut(s) 28, 171, 380, 456, 643
BseGI GGATG 6 cut(s) 181, 301, 346, 412, 603, 1045
BseLI CCNNNNNNNGG 3 cut(s) 292, 962, 1162
BseMI GCAATG 2 cut(s) 630, 1272
BseMII CTCAG 3 cut(s) 665, 901, 1296
BseNI ACTGG 3 cut(s) 47, 722, 859
BseRI GAGGAG 1 cut(s) 1007
BseXI GCAGC 6 cut(s) 363, 542, 721, 826, 997, 1079
BseYI CCCAGC 2 cut(s) 681, 955
Bsh1236I CGCG 7 cut(s) 37, 39, 51, 66, 104, 382, 1090
BshFI GGCC 6 cut(s) 336, 791, 858, 1068, 1099, 1241
BshNI GGYRCC 1 cut(s) 45
BsiHKAI GWGCWC 1 cut(s) 652
BsiSI CCGG 3 cut(s) 224, 338, 1096
BslFI GGGAC 1 cut(s) 656
BslI CCNNNNNNNGG 3 cut(s) 292, 962, 1162
BsmAI GTCTC 2 cut(s) 1138, 1285
BsmBI CGTCTC 1 cut(s) 1138
BsmFI GGGAC 1 cut(s) 656
BsnI GGCC 6 cut(s) 336, 791, 858, 1068, 1099, 1241
Bsp1286I GDGCHC 1 cut(s) 652
Bsp13I TCCGGA 1 cut(s) 223
Bsp143I GATC 9 cut(s) 145, 234, 361, 504, 600, 756, 906, 965, 1251
Bsp68I TCGCGA 1 cut(s) 104
BspACI CCGC 4 cut(s) 49, 380, 382, 1141
BspANI GGCC 6 cut(s) 336, 791, 858, 1068, 1099, 1241
BspCNI CTCAG 3 cut(s) 664, 900, 1295
BspEI TCCGGA 1 cut(s) 223
BspFNI CGCG 7 cut(s) 37, 39, 51, 66, 104, 382, 1090
BspHI TCATGA 1 cut(s) 937
BspLI GGNNCC 2 cut(s) 47, 672
BspPI GGATC 3 cut(s) 356, 914, 973
BspT107I GGYRCC 1 cut(s) 45
BsrDI GCAATG 2 cut(s) 630, 1272
BsrFI RCCGGY 1 cut(s) 1095
BsrI ACTGG 3 cut(s) 47, 722, 859
BssAI RCCGGY 1 cut(s) 1095
BssECI CCNNGG 5 cut(s) 28, 171, 380, 456, 643
BssMI GATC 9 cut(s) 145, 234, 361, 504, 600, 756, 906, 965, 1251
BssNI GRCGYC 1 cut(s) 1147
BssT1I CCWWGG 2 cut(s) 456, 643
Bst2UI CCWGG 3 cut(s) 13, 366, 1243
Bst4CI ACNGT 5 cut(s) 372, 537, 983, 1161, 1320
Bst6I CTCTTC 3 cut(s) 225, 591, 921
BstACI GRCGYC 1 cut(s) 1147
BstC8I GCNNGC 4 cut(s) 35, 384, 819, 1097
BstDEI CTNAG 3 cut(s) 651, 887, 1282
BstDSI CCRYGG 2 cut(s) 28, 380
BstEII GGTNACC 2 cut(s) 638, 1262
BstF5I GGATG 6 cut(s) 181, 301, 346, 412, 603, 1045
BstFNI CGCG 7 cut(s) 37, 39, 51, 66, 104, 382, 1090
BstHHI GCGC 4 cut(s) 39, 53, 66, 1092
BstKTI GATC 9 cut(s) 148, 237, 364, 507, 603, 759, 909, 968, 1254
BstMAI GTCTC 2 cut(s) 1138, 1285
BstMBI GATC 9 cut(s) 145, 234, 361, 504, 600, 756, 906, 965, 1251
BstMWI GCNNNNNNNGC 7 cut(s) 43, 150, 629, 718, 788, 1019, 1096
BstNI CCWGG 3 cut(s) 13, 366, 1243
BstNSI RCATGY 1 cut(s) 25
BstPI GGTNACC 2 cut(s) 638, 1262
BstSCI CCNGG 4 cut(s) 11, 336, 364, 1241
BstSFI CTRYAG 1 cut(s) 1157
BstUI CGCG 7 cut(s) 37, 39, 51, 66, 104, 382, 1090
BstV1I GCAGC 6 cut(s) 363, 542, 721, 826, 997, 1079
BsuI GTATCC 2 cut(s) 109, 774
BsuRI GGCC 6 cut(s) 336, 791, 858, 1068, 1099, 1241
BtgI CCRYGG 2 cut(s) 28, 380
BtgZI GCGATG 2 cut(s) 294, 457
BtsCI GGATG 6 cut(s) 181, 301, 346, 412, 603, 1045
BtsI GCAGTG 1 cut(s) 1229
BtsIMutI CAGTG 3 cut(s) 202, 1166, 1229
BtuMI TCGCGA 1 cut(s) 104
Cac8I GCNNGC 4 cut(s) 35, 384, 819, 1097
CciI TCATGA 1 cut(s) 937
CfoI GCGC 4 cut(s) 39, 53, 66, 1092
Cfr10I RCCGGY 1 cut(s) 1095
Cfr13I GGNCC 3 cut(s) 335, 670, 856
Cfr42I CCGCGG 1 cut(s) 383
Csp6I GTAC 2 cut(s) 357, 1316
CviAII CATG 5 cut(s) 22, 139, 436, 938, 1072
CviQI GTAC 2 cut(s) 357, 1316
DdeI CTNAG 3 cut(s) 651, 887, 1282
DpnI GATC 9 cut(s) 147, 236, 363, 506, 602, 758, 908, 967, 1253
DpnII GATC 9 cut(s) 145, 234, 361, 504, 600, 756, 906, 965, 1251
EaeI YGGCCR 1 cut(s) 789
Eam1104I CTCTTC 3 cut(s) 225, 591, 921
EarI CTCTTC 3 cut(s) 225, 591, 921
Eco130I CCWWGG 2 cut(s) 456, 643
Eco47I GGWCC 1 cut(s) 670
Eco57I CTGAAG 4 cut(s) 519, 804, 822, 1256
Eco91I GGTNACC 2 cut(s) 638, 1262
EcoO109I RGGNCCY 1 cut(s) 670
EcoO65I GGTNACC 2 cut(s) 638, 1262
EcoRII CCWGG 3 cut(s) 11, 364, 1241
EcoT14I CCWWGG 2 cut(s) 456, 643
ErhI CCWWGG 2 cut(s) 456, 643
Esp3I CGTCTC 1 cut(s) 1138
FaeI CATG 5 cut(s) 25, 142, 439, 941, 1075
FaqI GGGAC 1 cut(s) 656
FatI CATG 5 cut(s) 21, 138, 435, 937, 1071
FauI CCCGC 1 cut(s) 375
FbaI TGATCA 2 cut(s) 504, 1251
Fnu4HI GCNGC 7 cut(s) 377, 380, 531, 710, 815, 1011, 1093
FokI GGATG 6 cut(s) 188, 308, 353, 419, 590, 1052
Fsp4HI GCNGC 7 cut(s) 377, 380, 531, 710, 815, 1011, 1093
FspBI CTAG 3 cut(s) 540, 644, 777
GlaI GCGC 4 cut(s) 38, 52, 65, 1091
GluI GCNGC 7 cut(s) 377, 380, 531, 710, 815, 1011, 1093
GsaI CCCAGC 2 cut(s) 685, 959
GsuI CTGGAG 1 cut(s) 421
HaeIII GGCC 6 cut(s) 336, 791, 858, 1068, 1099, 1241
HapII CCGG 3 cut(s) 224, 338, 1096
HhaI GCGC 4 cut(s) 39, 53, 66, 1092
Hin1I GRCGYC 1 cut(s) 1147
Hin1II CATG 5 cut(s) 25, 142, 439, 941, 1075
Hin6I GCGC 4 cut(s) 37, 51, 64, 1090
HinP1I GCGC 4 cut(s) 37, 51, 64, 1090
HindIII AAGCTT 1 cut(s) 329
HinfI GANTC 6 cut(s) 133, 578, 749, 845, 1171, 1246
HpaII CCGG 3 cut(s) 224, 338, 1096
HphI GGTGA 5 cut(s) 191, 319, 650, 854, 1274
Hpy188III TCNNGA 5 cut(s) 103, 224, 400, 910, 938
Hpy99I CGWCG 2 cut(s) 11, 104
HpyAV CCTTC 4 cut(s) 116, 1223, 1231, 1234
HpyCH4III ACNGT 5 cut(s) 372, 537, 983, 1161, 1320
HpyCH4IV ACGT 3 cut(s) 6, 934, 1147
HpyCH4V TGCA 3 cut(s) 530, 611, 1294
HpyF10VI GCNNNNNNNGC 7 cut(s) 43, 150, 629, 718, 788, 1019, 1096
HpyF3I CTNAG 3 cut(s) 651, 887, 1282
HpySE526I ACGT 3 cut(s) 6, 934, 1147
Hsp92I GRCGYC 1 cut(s) 1147
Hsp92II CATG 5 cut(s) 25, 142, 439, 941, 1075
HspAI GCGC 4 cut(s) 37, 51, 64, 1090
Kpn2I TCCGGA 1 cut(s) 223
KroI GCCGGC 1 cut(s) 1095
KroNI GCCGGC 1 cut(s) 1097
Ksp22I TGATCA 2 cut(s) 504, 1251
KspI CCGCGG 1 cut(s) 383
Kzo9I GATC 9 cut(s) 145, 234, 361, 504, 600, 756, 906, 965, 1251
LmnI GCTCC 2 cut(s) 620, 994
Lsp1109I GCAGC 6 cut(s) 363, 542, 721, 826, 997, 1079
LweI GCATC 5 cut(s) 620, 814, 822, 1030, 1085
MaeI CTAG 3 cut(s) 540, 644, 777
MaeII ACGT 3 cut(s) 6, 934, 1147
MaeIII GTNAC 3 cut(s) 638, 948, 1262
MalI GATC 9 cut(s) 147, 236, 363, 506, 602, 758, 908, 967, 1253
MboI GATC 9 cut(s) 145, 234, 361, 504, 600, 756, 906, 965, 1251
MboII GAAGA 5 cut(s) 212, 275, 444, 608, 938
MhlI GDGCHC 1 cut(s) 652
MluCI AATT 3 cut(s) 244, 447, 1027
MlyI GAGTC 2 cut(s) 572, 854
MroI TCCGGA 1 cut(s) 223
MroNI GCCGGC 1 cut(s) 1095
MseI TTAA 1 cut(s) 516
MspA1I CMGCKG 1 cut(s) 382
MspI CCGG 3 cut(s) 224, 338, 1096
MspR9I CCNGG 4 cut(s) 13, 338, 366, 1243
MvaI CCWGG 3 cut(s) 13, 366, 1243
MvnI CGCG 7 cut(s) 37, 39, 51, 66, 104, 382, 1090
MwoI GCNNNNNNNGC 7 cut(s) 43, 150, 629, 718, 788, 1019, 1096
NaeI GCCGGC 1 cut(s) 1097
NciI CCSGG 1 cut(s) 338
NdeII GATC 9 cut(s) 145, 234, 361, 504, 600, 756, 906, 965, 1251
NgoMIV GCCGGC 1 cut(s) 1095
NlaIII CATG 5 cut(s) 25, 142, 439, 941, 1075
NlaIV GGNNCC 2 cut(s) 47, 672
NmeAIII GCCGAG 2 cut(s) 129, 196
NmuCI GTSAC 2 cut(s) 638, 1262
NruI TCGCGA 1 cut(s) 104
NspI RCATGY 1 cut(s) 25
PagI TCATGA 1 cut(s) 937
PciI ACATGT 1 cut(s) 21
PcsI WCGNNNNNNNCGW 1 cut(s) 36
PdiI GCCGGC 1 cut(s) 1097
PfeI GAWTC 4 cut(s) 133, 749, 1171, 1246
PflFI GACNNNGTC 1 cut(s) 1268
PflMI CCANNNNNTGG 1 cut(s) 962
PfoI TCCNGGA 1 cut(s) 364
PkrI GCNGC 7 cut(s) 378, 381, 532, 711, 816, 1012, 1094
PleI GAGTC 2 cut(s) 572, 853
PpsI GAGTC 2 cut(s) 572, 853
PpuMI RGGWCCY 1 cut(s) 670
PscI ACATGT 1 cut(s) 21
Psp5II RGGWCCY 1 cut(s) 670
Psp6I CCWGG 3 cut(s) 11, 364, 1241
PspEI GGTNACC 2 cut(s) 638, 1262
PspFI CCCAGC 2 cut(s) 681, 955
PspGI CCWGG 3 cut(s) 11, 364, 1241
PspN4I GGNNCC 2 cut(s) 47, 672
PspPI GGNCC 3 cut(s) 335, 670, 856
PspPPI RGGWCCY 1 cut(s) 670
PsrI GAACNNNNNNTAC 2 cut(s) 933, 965
PsyI GACNNNGTC 1 cut(s) 1268
RruI TCGCGA 1 cut(s) 104
RsaI GTAC 2 cut(s) 358, 1317
RsaNI GTAC 2 cut(s) 357, 1316
SacII CCGCGG 1 cut(s) 383
SaqAI TTAA 1 cut(s) 516
SatI GCNGC 7 cut(s) 377, 380, 531, 710, 815, 1011, 1093
Sau3AI GATC 9 cut(s) 145, 234, 361, 504, 600, 756, 906, 965, 1251
Sau96I GGNCC 3 cut(s) 335, 670, 856
SchI GAGTC 2 cut(s) 572, 854
ScrFI CCNGG 4 cut(s) 13, 338, 366, 1243
SduI GDGCHC 1 cut(s) 652
SfaNI GCATC 5 cut(s) 620, 814, 822, 1030, 1085
SfcI CTRYAG 1 cut(s) 1157
Sfr303I CCGCGG 1 cut(s) 383
SgrBI CCGCGG 1 cut(s) 383
SinI GGWCC 1 cut(s) 670
Sse9I AATT 3 cut(s) 244, 447, 1027
SsiI CCGC 4 cut(s) 49, 380, 382, 1141
SspMI CTAG 3 cut(s) 540, 644, 777
StyD4I CCNGG 4 cut(s) 11, 336, 364, 1241
StyI CCWWGG 2 cut(s) 456, 643
TaaI ACNGT 5 cut(s) 372, 537, 983, 1161, 1320
TaiI ACGT 3 cut(s) 9, 937, 1150
TaqI TCGA 3 cut(s) 747, 759, 909
TasI AATT 3 cut(s) 244, 447, 1027
TatI WGTACW 1 cut(s) 356
TauI GCSGC 1 cut(s) 382
TfiI GAWTC 4 cut(s) 133, 749, 1171, 1246
Tru1I TTAA 1 cut(s) 516
Tru9I TTAA 1 cut(s) 516
TscAI CASTG 3 cut(s) 202, 1166, 1236
TseFI GTSAC 2 cut(s) 638, 1262
TseI GCWGC 6 cut(s) 376, 530, 709, 814, 1010, 1092
Tsp45I GTSAC 2 cut(s) 638, 1262
TspDTI ATGAA 4 cut(s) 17, 460, 954, 1039
TspGWI ACGGA 1 cut(s) 151
TspRI CASTG 3 cut(s) 202, 1166, 1236
Tth111I GACNNNGTC 1 cut(s) 1268
Van91I CCANNNNNTGG 1 cut(s) 962
VpaK11BI GGWCC 1 cut(s) 670
XceI RCATGY 1 cut(s) 25
XmaJI CCTAGG 1 cut(s) 643
XspI CTAG 3 cut(s) 540, 644, 777
ZraI GACGTC 1 cut(s) 1148
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.