Rroxscaffold_5G00335190

Belongs to the pyruvate kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0009431
Physical Location & Seq
Forward (+)
2618830 .. 2625054
6225 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00335190.1

Sequence Viewer

Length: 1740 bp
ATGTCTCACTCCCTTCACCTCCTCACCCCCACCACCTTAACCTTCCCCAAACCCCATTCCTCCTCCTCCTCCTCCTCATTCCTCAACCCCTTCTCCACCGCCTATCGCCGGTCTCCGGTCACCACCGGCTTCCCCACCTTCTCCATCAAAGCCTCCGCCGCGGATCAAAAACCCTCCGTCCTCGTCTCCGGCAACGGCACCTCCTCCTCCGCGGTACAATCCCAGCAGGACTATGCCTCCTCCACCTCCATCGAGGTCGACGCCGTCACCGAGGCCGAGCTCCGGGAGAACGGCTTCCGAAGCACGCGGCGGACGAAGCTCGTCTGCACCATCGGCCCCGCCACCGCCGGCTTCGACCAGCTCGAGGCCCTCGCCGTCGGCGGCATGAACGTCGCCAGGCTCAACATGTGCCACGGCACGCGCGACTGGCACCGCGCCGTGATTGAGCGCGTGAGGAAGCTCAACGAGGACAAGGGCTACGCCGTCGCGATTATGATGGATACCGAAGGGAGTGAGATTCACATGGGCGATCTCGGCACTTCCTCCGTCAAAGCCGAGGATGGTGAGATATGGACTTTCAGTGTCAGAGCGTTCGGTTCTACTCGTCCGGAACGCACGATCAATGTGAATTACGAGGGTTTCGCCGAAGATGTGAAAGTGGGGGATGAGCTACTTGTGGATGGTGGAATGGTGAGGTTTGAGGTGGTTGAGAAGCTTGGCCCGGATGTCAAGTGTAGATGTACTGATCCTGGACTGTTGCTGCCGCGGGCTAACTTGACTTTCTGGAGGGATGGGAGTTTAGTGCGAGAACGAAATGCCATGCTTCCCACAATTTCTTCCAAGGATTGGTTGGACATTGATTTCGGGATTGCTGAGGGTGTTGATTTCATCGCTGTATCTTTTGTCAAGTCTGCTGAAGTGATCAATCATCTTAAAAGCTATATTGCAGCACGGTCTAGCGATATTTCTGTTGTGGCAAAGATAGAGAGTATTGACTCTCTAAAGAACTTGGAAGAGATCATCCTTGCATCAGATGGAGCAATGGTAGCAAGGGGTGACCTAGGTGCTCAGATACCATTGGAACAGGTCCCATCAGCCCAGCAAAAGATTGTTCAAGTATGTCGGCAGCTAAATAAGCCAGTTATCGTTGCTTCTCAACTACTCGAATCTATGATCGAGTATCCTACACCCACTAGAGCTGAAGTGGCCGATGTTTCTGAAGCCGTGAGGCAGCGGGCTGATGCTCTGATGCTTTCTGGTGAGTCAGCTATGGGCCAGTTCCCAGATAAGTCATTAGCTGTTCTCAGAAGTGTCAGTTTGAGGATCGAGAGGTGGTGGAGGGAAGAGAAACGTCATGAACCTATGTTACTCCCAGCTATTGGATCATCATTTTCAGACAGTATATCGGAGGAGCTTTGTATTTCTGCTGCCAAGATGGCAAATAATTTAGAGGTGGATGCTCTTTTCATCTTTACAAAGACGGGCCACATGGCATCTCTCCTGTCGCGCTGCCGGCCTGACTGCCCAATCTTTGCTTTTACCAACACAACATCTGTGCGGAGACGTCTGAACCTACAGTGGGGGCTGATACCATTTCGTGTTAGTTTCTCTGATGATATGGAAAGCAACCTTCACAAGACCTTCTCACTGCTGAAGGCCAGGAATCTGATCAAATCAGGTGACCTTGTCATTGCTGTCTCAGACATATTGCAGTCCATTCAAGTTATGAATGTACCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000287 GO:0003006 GO:0003674 GO:0003824 GO:0004743 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006629 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009791 GO:0009987 GO:0010035 GO:0010038 GO:0010154 GO:0010431 GO:0016052 GO:0016053 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0021700 GO:0022414 GO:0030955 GO:0031420 GO:0032501 GO:0032502 GO:0032504 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042221 GO:0042866 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046686 GO:0046700 GO:0046872 GO:0046939 GO:0048316 GO:0048608 GO:0048609 GO:0048731 GO:0048856 GO:0050896 GO:0051186 GO:0051188 GO:0055086 GO:0061458 GO:0071695 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00230 ko00620 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko04922 ko04930 ko05165 ko05203 ko05230 map00010 map00230 map00620 map01100 map01110 map01120 map01130 map01200 map01230 map04922 map04930 map05165 map05203 map05230
Pfam Domains
Protein Families
PK PK_C

Protein Analysis

579

Amino Acids

63.5

Weight (kDa)

5.81

Isoelectric Point (pI)

46.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK PF00224 103 - 431 5e-91 Pyruvate kinase, barrel domain
PK_C PF02887 470 - 568 2.3e-23 Pyruvate kinase, alpha/beta domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1567
AccB1I GGYRCC 2 cut(s) 197, 429
AccB7I CCANNNNNTGG 2 cut(s) 846, 1379
AccI GTMKAC 1 cut(s) 258
AccIII TCCGGA 1 cut(s) 607
AclWI GGATC 4 cut(s) 171, 740, 1331, 1390
AcoI YGGCCR 1 cut(s) 1206
AcuI CTGAAG 4 cut(s) 936, 1221, 1239, 1673
AcyI GRCGYC 2 cut(s) 261, 1564
AfaI GTAC 3 cut(s) 216, 742, 1734
AfiI CCNNNNNNNGG 7 cut(s) 108, 115, 282, 364, 846, 1379, 1579
AflIII ACRYGT 1 cut(s) 405
AgsI TTSAA 2 cut(s) 1115, 1721
AjnI CCWGG 3 cut(s) 395, 748, 1658
Alw21I GWGCWC 2 cut(s) 282, 1069
Alw26I GTCTC 5 cut(s) 9, 117, 190, 1555, 1702
AlwI GGATC 4 cut(s) 171, 740, 1331, 1390
Ama87I CYCGRG 1 cut(s) 362
Aor13HI TCCGGA 1 cut(s) 607
AoxI GGCC 9 cut(s) 273, 334, 366, 718, 1206, 1273, 1483, 1514, 1656
ApeKI GCWGC 6 cut(s) 760, 947, 1126, 1231, 1427, 1509
ArsI GACNNNNNNTTYG 2 cut(s) 845, 877
Asp700I GAANNNNTTC 1 cut(s) 293
AspA2I CCTAGG 1 cut(s) 1060
AspLEI GCGC 4 cut(s) 423, 437, 450, 1509
AspS9I GGNCC 6 cut(s) 335, 367, 719, 1087, 1273, 1483
AsuC2I CCSGG 2 cut(s) 284, 722
AsuHPI GGTGA 9 cut(s) 8, 16, 112, 259, 575, 703, 1067, 1271, 1691
AvaI CYCGRG 1 cut(s) 362
AvaII GGWCC 1 cut(s) 1087
AvrII CCTAGG 1 cut(s) 1060
BanI GGYRCC 2 cut(s) 197, 429
BanII GRGCYC 1 cut(s) 282
Bbv12I GWGCWC 2 cut(s) 282, 1069
BbvCI CCTCAGC 1 cut(s) 873
BbvI GCAGC 6 cut(s) 747, 959, 1138, 1243, 1414, 1496
BceAI ACGGC 8 cut(s) 211, 248, 307, 359, 422, 430, 467, 1208
BcgI CGANNNNNNTGC 2 cut(s) 373, 407
BciT130I CCWGG 3 cut(s) 397, 750, 1660
BciVI GTATCC 2 cut(s) 493, 1191
BclI TGATCA 2 cut(s) 921, 1668
BcnI CCSGG 2 cut(s) 284, 722
BcoDI GTCTC 5 cut(s) 9, 117, 190, 1555, 1702
BfaI CTAG 3 cut(s) 957, 1061, 1194
BfmI CTRYAG 1 cut(s) 1574
BfuI GTATCC 2 cut(s) 493, 1191
BglI GCCNNNNNGGC 1 cut(s) 1436
BlnI CCTAGG 1 cut(s) 1060
Bme1390I CCNGG 5 cut(s) 284, 397, 722, 750, 1660
Bme18I GGWCC 1 cut(s) 1087
BmeT110I CYCGRG 1 cut(s) 362
BmgT120I GGNCC 6 cut(s) 335, 367, 719, 1087, 1273, 1483
BmiI GGNNCC 4 cut(s) 199, 337, 431, 1089
BmrFI CCNGG 5 cut(s) 284, 397, 722, 750, 1660
BmsI GCATC 5 cut(s) 1037, 1231, 1239, 1447, 1502
BpmI CTGGAG 1 cut(s) 805
Bpu10I CCTNAGC 1 cut(s) 873
BpuMI CCSGG 2 cut(s) 284, 722
BsaHI GRCGYC 2 cut(s) 261, 1564
BsaI GGTCTC 1 cut(s) 117
BsaJI CCNNGG 8 cut(s) 159, 210, 270, 412, 555, 764, 840, 1060
BsaWI WCCGGW 2 cut(s) 115, 607
BsaXI ACNNNNNCTCC 8 cut(s) 77, 107, 185, 215, 221, 251, 1029, 1059
Bsc4I CCNNNNNNNGG 7 cut(s) 108, 115, 282, 364, 846, 1379, 1579
Bse118I RCCGGY 4 cut(s) 108, 125, 347, 1512
Bse1I ACTGG 3 cut(s) 431, 1139, 1276
Bse3DI GCAATG 2 cut(s) 1047, 1689
BseAI TCCGGA 1 cut(s) 607
BseBI CCWGG 3 cut(s) 397, 750, 1660
BseDI CCNNGG 8 cut(s) 159, 210, 270, 412, 555, 764, 840, 1060
BseGI GGATG 7 cut(s) 565, 670, 685, 730, 796, 1020, 1462
BseLI CCNNNNNNNGG 7 cut(s) 108, 115, 282, 364, 846, 1379, 1579
BseMI GCAATG 2 cut(s) 1047, 1689
BseMII CTCAG 4 cut(s) 864, 1082, 1318, 1713
BseNI ACTGG 3 cut(s) 431, 1139, 1276
BseXI GCAGC 6 cut(s) 747, 959, 1138, 1243, 1414, 1496
BseYI CCCAGC 3 cut(s) 222, 1098, 1372
BsgI GTGCAG 1 cut(s) 310
BshFI GGCC 9 cut(s) 275, 336, 368, 720, 1208, 1275, 1485, 1516, 1658
BshNI GGYRCC 2 cut(s) 197, 429
BsiHKAI GWGCWC 2 cut(s) 282, 1069
BsiHKCI CYCGRG 1 cut(s) 362
BsiSI CCGG 9 cut(s) 109, 116, 126, 189, 283, 348, 608, 722, 1513
BslFI GGGAC 1 cut(s) 1073
BslI CCNNNNNNNGG 7 cut(s) 108, 115, 282, 364, 846, 1379, 1579
BsmAI GTCTC 5 cut(s) 9, 117, 190, 1555, 1702
BsmBI CGTCTC 2 cut(s) 190, 1555
BsmFI GGGAC 1 cut(s) 1073
BsnI GGCC 9 cut(s) 275, 336, 368, 720, 1208, 1275, 1485, 1516, 1658
Bso31I GGTCTC 1 cut(s) 117
BsoBI CYCGRG 1 cut(s) 362
Bsp1286I GDGCHC 2 cut(s) 282, 1069
Bsp13I TCCGGA 1 cut(s) 607
Bsp68I TCGCGA 1 cut(s) 488
BspANI GGCC 9 cut(s) 275, 336, 368, 720, 1208, 1275, 1485, 1516, 1658
BspCNI CTCAG 4 cut(s) 865, 1081, 1317, 1712
BspEI TCCGGA 1 cut(s) 607
BspHI TCATGA 1 cut(s) 1354
BspLI GGNNCC 4 cut(s) 199, 337, 431, 1089
BspPI GGATC 4 cut(s) 171, 740, 1331, 1390
BspT107I GGYRCC 2 cut(s) 197, 429
BspTNI GGTCTC 1 cut(s) 117
BsrDI GCAATG 2 cut(s) 1047, 1689
BsrFI RCCGGY 4 cut(s) 108, 125, 347, 1512
BsrI ACTGG 3 cut(s) 431, 1139, 1276
BssAI RCCGGY 4 cut(s) 108, 125, 347, 1512
BssECI CCNNGG 8 cut(s) 159, 210, 270, 412, 555, 764, 840, 1060
BssNI GRCGYC 2 cut(s) 261, 1564
BssT1I CCWWGG 2 cut(s) 840, 1060
Bst2UI CCWGG 3 cut(s) 397, 750, 1660
Bst4CI ACNGT 4 cut(s) 756, 954, 1400, 1578
Bst6I CTCTTC 2 cut(s) 1008, 1338
BstACI GRCGYC 2 cut(s) 261, 1564
BstC8I GCNNGC 6 cut(s) 305, 349, 419, 768, 1236, 1514
BstDEI CTNAG 4 cut(s) 873, 1068, 1304, 1699
BstDSI CCRYGG 4 cut(s) 159, 210, 412, 764
BstEII GGTNACC 3 cut(s) 118, 1055, 1679
BstF5I GGATG 7 cut(s) 565, 670, 685, 730, 796, 1020, 1462
BstHHI GCGC 4 cut(s) 423, 437, 450, 1509
BstMAI GTCTC 5 cut(s) 9, 117, 190, 1555, 1702
BstNI CCWGG 3 cut(s) 397, 750, 1660
BstNSI RCATGY 1 cut(s) 409
BstPI GGTNACC 3 cut(s) 118, 1055, 1679
BstSCI CCNGG 5 cut(s) 282, 395, 720, 748, 1658
BstSFI CTRYAG 1 cut(s) 1574
BstV1I GCAGC 6 cut(s) 747, 959, 1138, 1243, 1414, 1496
BsuI GTATCC 2 cut(s) 493, 1191
BsuRI GGCC 9 cut(s) 275, 336, 368, 720, 1208, 1275, 1485, 1516, 1658
BtgI CCRYGG 4 cut(s) 159, 210, 412, 764
BtgZI GCGATG 1 cut(s) 874
BtsCI GGATG 7 cut(s) 565, 670, 685, 730, 796, 1020, 1462
BtsI GCAGTG 1 cut(s) 1646
BtsIMutI CAGTG 3 cut(s) 586, 1583, 1646
BtuMI TCGCGA 1 cut(s) 488
Cac8I GCNNGC 6 cut(s) 305, 349, 419, 768, 1236, 1514
CciI TCATGA 1 cut(s) 1354
CfoI GCGC 4 cut(s) 423, 437, 450, 1509
Cfr10I RCCGGY 4 cut(s) 108, 125, 347, 1512
Cfr13I GGNCC 6 cut(s) 335, 367, 719, 1087, 1273, 1483
Cfr42I CCGCGG 3 cut(s) 162, 213, 767
CseI GACGC 1 cut(s) 269
Csp6I GTAC 3 cut(s) 215, 741, 1733
CviAII CATG 6 cut(s) 385, 406, 523, 820, 1355, 1489
CviQI GTAC 3 cut(s) 215, 741, 1733
DdeI CTNAG 4 cut(s) 873, 1068, 1304, 1699
EaeI YGGCCR 1 cut(s) 1206
Eam1104I CTCTTC 2 cut(s) 1008, 1338
EarI CTCTTC 2 cut(s) 1008, 1338
EciI GGCGGA 2 cut(s) 145, 325
Ecl136II GAGCTC 1 cut(s) 280
Eco130I CCWWGG 2 cut(s) 840, 1060
Eco24I GRGCYC 1 cut(s) 282
Eco31I GGTCTC 1 cut(s) 117
Eco47I GGWCC 1 cut(s) 1087
Eco53kI GAGCTC 1 cut(s) 280
Eco57I CTGAAG 4 cut(s) 936, 1221, 1239, 1673
Eco88I CYCGRG 1 cut(s) 362
Eco91I GGTNACC 3 cut(s) 118, 1055, 1679
EcoICRI GAGCTC 1 cut(s) 280
EcoO109I RGGNCCY 2 cut(s) 367, 1087
EcoO65I GGTNACC 3 cut(s) 118, 1055, 1679
EcoRII CCWGG 3 cut(s) 395, 748, 1658
EcoT14I CCWWGG 2 cut(s) 840, 1060
EcoT38I GRGCYC 1 cut(s) 282
ErhI CCWWGG 2 cut(s) 840, 1060
Esp3I CGTCTC 2 cut(s) 190, 1555
FaeI CATG 6 cut(s) 388, 409, 526, 823, 1358, 1492
FaqI GGGAC 1 cut(s) 1073
FatI CATG 6 cut(s) 384, 405, 522, 819, 1354, 1488
FauI CCCGC 3 cut(s) 346, 759, 1227
FbaI TGATCA 2 cut(s) 921, 1668
FblI GTMKAC 1 cut(s) 258
FokI GGATG 7 cut(s) 572, 677, 692, 737, 803, 1007, 1469
FriOI GRGCYC 1 cut(s) 282
FspBI CTAG 3 cut(s) 957, 1061, 1194
GlaI GCGC 4 cut(s) 422, 436, 449, 1508
GsaI CCCAGC 3 cut(s) 226, 1102, 1376
GsuI CTGGAG 1 cut(s) 805
HaeIII GGCC 9 cut(s) 275, 336, 368, 720, 1208, 1275, 1485, 1516, 1658
HapII CCGG 9 cut(s) 109, 116, 126, 189, 283, 348, 608, 722, 1513
HgaI GACGC 1 cut(s) 269
HhaI GCGC 4 cut(s) 423, 437, 450, 1509
Hin1I GRCGYC 2 cut(s) 261, 1564
Hin1II CATG 6 cut(s) 388, 409, 526, 823, 1358, 1492
Hin6I GCGC 4 cut(s) 421, 435, 448, 1507
HinP1I GCGC 4 cut(s) 421, 435, 448, 1507
HincII GTYRAC 1 cut(s) 259
HindII GTYRAC 1 cut(s) 259
HindIII AAGCTT 1 cut(s) 713
HinfI GANTC 5 cut(s) 517, 995, 1166, 1262, 1663
HpaII CCGG 9 cut(s) 109, 116, 126, 189, 283, 348, 608, 722, 1513
HphI GGTGA 9 cut(s) 8, 16, 112, 259, 575, 703, 1067, 1271, 1691
Hpy166II GTNNAC 1 cut(s) 259
Hpy188III TCNNGA 6 cut(s) 487, 608, 784, 865, 1327, 1355
Hpy8I GTNNAC 1 cut(s) 259
Hpy99I CGWCG 4 cut(s) 263, 380, 395, 488
HpyAV CCTTC 8 cut(s) 23, 52, 100, 148, 500, 1640, 1648, 1651
HpyCH4III ACNGT 4 cut(s) 756, 954, 1400, 1578
HpyCH4IV ACGT 3 cut(s) 390, 1351, 1564
HpyCH4V TGCA 4 cut(s) 327, 947, 1028, 1711
HpyF3I CTNAG 4 cut(s) 873, 1068, 1304, 1699
HpySE526I ACGT 3 cut(s) 390, 1351, 1564
Hsp92I GRCGYC 2 cut(s) 261, 1564
Hsp92II CATG 6 cut(s) 388, 409, 526, 823, 1358, 1492
HspAI GCGC 4 cut(s) 421, 435, 448, 1507
Kpn2I TCCGGA 1 cut(s) 607
KroI GCCGGC 2 cut(s) 347, 1512
KroNI GCCGGC 2 cut(s) 349, 1514
Ksp22I TGATCA 2 cut(s) 921, 1668
KspI CCGCGG 3 cut(s) 162, 213, 767
LmnI GCTCC 3 cut(s) 285, 1037, 1411
Lsp1109I GCAGC 6 cut(s) 747, 959, 1138, 1243, 1414, 1496
LweI GCATC 5 cut(s) 1037, 1231, 1239, 1447, 1502
MaeI CTAG 3 cut(s) 957, 1061, 1194
MaeII ACGT 3 cut(s) 390, 1351, 1564
MaeIII GTNAC 5 cut(s) 118, 265, 1055, 1365, 1679
MboII GAAGA 4 cut(s) 659, 828, 1025, 1355
MhlI GDGCHC 2 cut(s) 282, 1069
MluCI AATT 3 cut(s) 628, 831, 1444
MlyI GAGTC 2 cut(s) 989, 1271
MmeI TCCRAC 1 cut(s) 831
MroI TCCGGA 1 cut(s) 607
MroNI GCCGGC 2 cut(s) 347, 1512
MroXI GAANNNNTTC 1 cut(s) 293
MseI TTAA 2 cut(s) 38, 933
MspA1I CMGCKG 4 cut(s) 161, 212, 766, 1234
MspI CCGG 9 cut(s) 109, 116, 126, 189, 283, 348, 608, 722, 1513
MspR9I CCNGG 5 cut(s) 284, 397, 722, 750, 1660
MvaI CCWGG 3 cut(s) 397, 750, 1660
NaeI GCCGGC 2 cut(s) 349, 1514
NciI CCSGG 2 cut(s) 284, 722
NgoMIV GCCGGC 2 cut(s) 347, 1512
NlaIII CATG 6 cut(s) 388, 409, 526, 823, 1358, 1492
NlaIV GGNNCC 4 cut(s) 199, 337, 431, 1089
NmeAIII GCCGAG 3 cut(s) 301, 513, 580
NmuCI GTSAC 4 cut(s) 118, 265, 1055, 1679
NruI TCGCGA 1 cut(s) 488
NspI RCATGY 1 cut(s) 409
PaeR7I CTCGAG 1 cut(s) 362
PagI TCATGA 1 cut(s) 1354
PciI ACATGT 1 cut(s) 405
PcsI WCGNNNNNNNCGW 4 cut(s) 267, 311, 360, 420
PdiI GCCGGC 2 cut(s) 349, 1514
PdmI GAANNNNTTC 1 cut(s) 293
PfeI GAWTC 3 cut(s) 517, 1166, 1663
PflFI GACNNNGTC 2 cut(s) 263, 1685
PflMI CCANNNNNTGG 2 cut(s) 846, 1379
PfoI TCCNGGA 2 cut(s) 282, 748
PleI GAGTC 2 cut(s) 989, 1270
PpsI GAGTC 2 cut(s) 989, 1270
PpuMI RGGWCCY 1 cut(s) 1087
PscI ACATGT 1 cut(s) 405
Psp124BI GAGCTC 1 cut(s) 282
Psp5II RGGWCCY 1 cut(s) 1087
Psp6I CCWGG 3 cut(s) 395, 748, 1658
PspEI GGTNACC 3 cut(s) 118, 1055, 1679
PspFI CCCAGC 3 cut(s) 222, 1098, 1372
PspGI CCWGG 3 cut(s) 395, 748, 1658
PspN4I GGNNCC 4 cut(s) 199, 337, 431, 1089
PspPI GGNCC 6 cut(s) 335, 367, 719, 1087, 1273, 1483
PspPPI RGGWCCY 1 cut(s) 1087
PspXI VCTCGAGB 1 cut(s) 362
PsrI GAACNNNNNNTAC 2 cut(s) 1350, 1382
PsyI GACNNNGTC 2 cut(s) 263, 1685
RruI TCGCGA 1 cut(s) 488
RsaI GTAC 3 cut(s) 216, 742, 1734
RsaNI GTAC 3 cut(s) 215, 741, 1733
SacI GAGCTC 1 cut(s) 282
SacII CCGCGG 3 cut(s) 162, 213, 767
SalI GTCGAC 1 cut(s) 257
SaqAI TTAA 2 cut(s) 38, 933
Sau96I GGNCC 6 cut(s) 335, 367, 719, 1087, 1273, 1483
SchI GAGTC 2 cut(s) 989, 1271
ScrFI CCNGG 5 cut(s) 284, 397, 722, 750, 1660
SduI GDGCHC 2 cut(s) 282, 1069
SfaNI GCATC 5 cut(s) 1037, 1231, 1239, 1447, 1502
SfcI CTRYAG 1 cut(s) 1574
Sfr274I CTCGAG 1 cut(s) 362
Sfr303I CCGCGG 3 cut(s) 162, 213, 767
SgrBI CCGCGG 3 cut(s) 162, 213, 767
SinI GGWCC 1 cut(s) 1087
SlaI CTCGAG 1 cut(s) 362
SmlI CTYRAG 1 cut(s) 362
SmoI CTYRAG 1 cut(s) 362
Sse9I AATT 3 cut(s) 628, 831, 1444
SspMI CTAG 3 cut(s) 957, 1061, 1194
SstI GAGCTC 1 cut(s) 282
StyD4I CCNGG 5 cut(s) 282, 395, 720, 748, 1658
StyI CCWWGG 2 cut(s) 840, 1060
TaaI ACNGT 4 cut(s) 756, 954, 1400, 1578
TaiI ACGT 3 cut(s) 393, 1354, 1567
TaqI TCGA 7 cut(s) 252, 258, 354, 363, 1164, 1176, 1326
TasI AATT 3 cut(s) 628, 831, 1444
TatI WGTACW 1 cut(s) 740
TauI GCSGC 4 cut(s) 161, 310, 384, 766
TfiI GAWTC 3 cut(s) 517, 1166, 1663
Tru1I TTAA 2 cut(s) 38, 933
Tru9I TTAA 2 cut(s) 38, 933
TscAI CASTG 3 cut(s) 586, 1583, 1653
TseFI GTSAC 4 cut(s) 118, 265, 1055, 1679
TseI GCWGC 6 cut(s) 760, 947, 1126, 1231, 1427, 1509
Tsp45I GTSAC 4 cut(s) 118, 265, 1055, 1679
TspDTI ATGAA 4 cut(s) 401, 877, 1371, 1456
TspGWI ACGGA 2 cut(s) 166, 535
TspRI CASTG 3 cut(s) 586, 1583, 1653
Tth111I GACNNNGTC 2 cut(s) 263, 1685
Van91I CCANNNNNTGG 2 cut(s) 846, 1379
VpaK11BI GGWCC 1 cut(s) 1087
XceI RCATGY 1 cut(s) 409
XcmI CCANNNNNNNNNTGG 1 cut(s) 847
XhoI CTCGAG 1 cut(s) 362
XmaJI CCTAGG 1 cut(s) 1060
XmiI GTMKAC 1 cut(s) 258
XmnI GAANNNNTTC 1 cut(s) 293
XspI CTAG 3 cut(s) 957, 1061, 1194
ZraI GACGTC 1 cut(s) 1565
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.