pycom16g17090

Belongs to the pyruvate kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0009431
Physical Location & Seq
Forward (+)
13132146 .. 13138188
6043 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g17090.1

Sequence Viewer

Length: 1941 bp
ATGCCCATCGGTCTCCAGTCGTTTTTGTCCCTCCTCCATTTTCCTCGCTTTGGAAACTCCTCCCAATTTCCCAAAGCTATCTCCCCTTCGGTAACCCCGCCCAACTATCCGCTTTTAAACGCCTCACCTTCTTCCTCCGTGACCGTACACCGCCGGTCAACACCAGTCACAATGTCGCAGTCCGTACATCTCCTCACCCCTTCCAACCTCGCCATCCCAAAACATCACTCCTCCTTCCTCTCCACCTTCAATCGCCGGTTGCCGGTCACCACCACCACCACAACCGCTTCCCCTTTCTTCCCAAAGCTCTCCATCAGAGCCTCCTCCTCCGATCGCAAGCCTTCCGTTCTCGTCACCGACAACGGCACATCCTCCTCGGGGCTCCAATCCTCTACTTCGCACGCCGACCATGCTCTCTCCAGCTCCATCGAGGTCGACGCGGTCACCGAAGCCGAGCTCCGGGAGAACGGATTCCGCTCCACCAGGCGCACCAAGCTCGTATGCACCATCGGTCCCGCCACCTCCGGATTCGACCAGCTCGAGTCGCTCGCCGTCGGCGGCATGAACGTGGCGCGCATCAACATGTGCCACGGCACCCGCGAGTGGCACCGCGAGGTCATTCAGCGCGTCAGGAGGCTCAACGTCGACAAGGGATACGCCGTCGCCATCATGATGGACACCGAAGGCAGCGAGATTCATATGGGTGATTTGGGTGGCGCCTCCTCCGCCAAAGCCGAGGATGGTGAAGTATGGACTTTTAGTGTAAGAGCTTTCGGCTCCACTCTCCCCCAGAACACCATCAATGTGAGTTATGAAGGTTTTGCTGAAGATGTGAAAGTGGGCGATGAACTGCTTGTGGATGGCGGGATGGTGAGGTTCGAGGTGATTGAGAAGCTTGGTCCTGATGTTAAGTGTAAGTGTACTGATCGATCACCACAACAAACTGGTTTGTTGCTGCCTCGGGCTGATTTGCCTTTCTGGAGGGACGGGAGTTTAGTGCGAGAACGCAATGCCATGCTTCCCACAATTTCTTCTAAGGATTGGTTGGACATTGATTTTGGGATTGCAGAGGGTGTTGATTTTATCGCTGTATCTTTTGTCAAGTCTGCGGAAGTGATTAATCATCTTAAAAGCTATATTGCTGCACGGTCACGAGATAGTGATGTTGCTGTTATTGCAAAGATAGAGAGTATTGACTCGCTAAAGAACTTGGAAGAGATCATTCAAGCATCAGATGGAGCAATGGTAGCAAGAGGAGATCTCGGTGCTCAGATACCACTAGAACAGGTCCCAGCAGCCCAGCAAAAGATTGTTCAAGTTTGTCGGCAGCTAAATAAGCCAGTCATTGTGGCTTCTCAACTACTTGAATCTATGATTGAATATCCTACGCCCACTAGAGCCGAAGTGGCTGATGTTTCTGAAGCAGTGAGGCAGCGCGCTGATGCTTTGATGCTTTCTGGTGAGTCGGCTATGGGCCAGTTCCCAGAAAAGTCATTGGCTGTTCTCAGAAGTGTTAGTTTGAGGATTGAAAGATGGTGGAGGGAAGAGAAACGTCATGAAGCTATGGAACTGCCAGCTATTGGAACTTCATTTTCTGACGGTATTTCAGAGGAGATTTGCATTTCTGCTGCCAAGATGGCCAATAATTTGGAGGTAGATGCTCTTTTTGTCTATACAAAGACAGGCCACATGGCATCTCTCTTGTCTCGCTGCCGGCCAGACTGCCCAATCTTTGCTTTTACTAACACGACATCTGTACGGAGACGTCTGAACCTACAGTGGGGTCTGATACCGTTTAGGGTGAGCTTCTCCGATGATATGGAGAGCAACCTTAATAAAACCTTCTCGTTGCTGAAGGCCAGGAATTTGATCGAGTCAGGCGACCTTGTTATTGCTGTCTCAGACGTCTTGCAGTGCATCCAAGTTATGAATGTTCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000287 GO:0003006 GO:0003674 GO:0003824 GO:0004743 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006629 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009791 GO:0009987 GO:0010035 GO:0010038 GO:0010154 GO:0010431 GO:0016052 GO:0016053 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0021700 GO:0022414 GO:0030955 GO:0031420 GO:0032501 GO:0032502 GO:0032504 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042221 GO:0042866 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046686 GO:0046700 GO:0046872 GO:0046939 GO:0048316 GO:0048608 GO:0048609 GO:0048731 GO:0048856 GO:0050896 GO:0051186 GO:0051188 GO:0055086 GO:0061458 GO:0071695 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00230 ko00620 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko04922 ko04930 ko05165 ko05203 ko05230 map00010 map00230 map00620 map01100 map01110 map01120 map01130 map01200 map01230 map04922 map04930 map05165 map05203 map05230
Pfam Domains
Protein Families
PK PK_C

Protein Analysis

647

Amino Acids

70.75

Weight (kDa)

6.16

Isoelectric Point (pI)

51.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK PF00224 162 - 498 1.5e-88 Pyruvate kinase, barrel domain
PK_C PF02887 537 - 635 4.2e-24 Pyruvate kinase, alpha/beta domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 1768, 1908
AccB1I GGYRCC 3 cut(s) 593, 606, 716
AccB7I CCANNNNNTGG 1 cut(s) 1580
AccBSI CCGCTC 1 cut(s) 477
AccI GTMKAC 2 cut(s) 435, 645
AccII CGCG 6 cut(s) 440, 574, 600, 612, 627, 1437
AccIII TCCGGA 1 cut(s) 524
AcoI YGGCCR 2 cut(s) 1638, 1715
AcsI RAATTY 1 cut(s) 1864
AcuI CTGAAG 3 cut(s) 846, 1440, 1874
AcyI GRCGYC 3 cut(s) 717, 1765, 1905
AfaI GTAC 4 cut(s) 147, 186, 922, 1758
AfiI CCNNNNNNNGG 7 cut(s) 50, 262, 378, 459, 603, 1580, 1780
AflIII ACRYGT 1 cut(s) 582
AgsI TTSAA 6 cut(s) 250, 1226, 1316, 1367, 1379, 1529
AjnI CCWGG 2 cut(s) 482, 1859
Alw21I GWGCWC 2 cut(s) 459, 1270
Alw26I GTCTC 4 cut(s) 17, 1710, 1756, 1903
Ama87I CYCGRG 3 cut(s) 376, 539, 960
Aor13HI TCCGGA 1 cut(s) 524
AoxI GGCC 5 cut(s) 1474, 1638, 1684, 1715, 1857
ApeKI GCWGC 8 cut(s) 687, 955, 1142, 1295, 1327, 1432, 1628, 1710
ApoI RAATTY 1 cut(s) 1864
ArsI GACNNNNNNTTYG 2 cut(s) 1040, 1072
AseI ATTAAT 1 cut(s) 1119
Asp700I GAANNNNTTC 1 cut(s) 470
AspLEI GCGC 7 cut(s) 489, 574, 576, 627, 719, 1437, 1439
AspS9I GGNCC 4 cut(s) 512, 899, 1288, 1474
AsuC2I CCSGG 1 cut(s) 461
AvaI CYCGRG 3 cut(s) 376, 539, 960
AvaII GGWCC 3 cut(s) 512, 899, 1288
BalI TGGCCA 1 cut(s) 1640
BanI GGYRCC 3 cut(s) 593, 606, 716
BanII GRGCYC 2 cut(s) 384, 459
BauI CACGAG 1 cut(s) 1152
Bbv12I GWGCWC 2 cut(s) 459, 1270
BbvI GCAGC 8 cut(s) 699, 942, 1129, 1307, 1339, 1444, 1615, 1697
BceAI ACGGC 4 cut(s) 379, 536, 607, 644
BcgI CGANNNNNNTGC 2 cut(s) 478, 512
BciT130I CCWGG 2 cut(s) 484, 1861
BciVI GTATCC 1 cut(s) 647
BcnI CCSGG 1 cut(s) 461
BcoDI GTCTC 4 cut(s) 17, 1710, 1756, 1903
BfaI CTAG 2 cut(s) 1280, 1395
BfmI CTRYAG 1 cut(s) 1775
BfoI RGCGCY 1 cut(s) 720
BfuI GTATCC 1 cut(s) 647
BglI GCCNNNNNGGC 2 cut(s) 1406, 1637
BglII AGATCT 1 cut(s) 1258
BisI GCNGC 9 cut(s) 559, 688, 956, 1143, 1296, 1328, 1433, 1629, 1711
BlsI GCNGC 9 cut(s) 560, 689, 957, 1144, 1297, 1329, 1434, 1630, 1712
Bme1390I CCNGG 3 cut(s) 461, 484, 1861
Bme18I GGWCC 3 cut(s) 512, 899, 1288
BmeT110I CYCGRG 3 cut(s) 376, 539, 960
BmgT120I GGNCC 4 cut(s) 512, 899, 1288, 1474
BmiI GGNNCC 7 cut(s) 383, 514, 595, 608, 718, 778, 1290
BmrFI CCNGG 3 cut(s) 461, 484, 1861
BmsI GCATC 7 cut(s) 585, 1238, 1432, 1440, 1648, 1703, 1926
BplI GAGNNNNNCTC 2 cut(s) 1245, 1277
BpmI CTGGAG 2 cut(s) 403, 1000
BpuMI CCSGG 1 cut(s) 461
Bsa29I ATCGAT 1 cut(s) 928
BsaHI GRCGYC 3 cut(s) 717, 1765, 1905
BsaI GGTCTC 1 cut(s) 17
BsaJI CCNNGG 4 cut(s) 375, 589, 735, 959
BsaWI WCCGGW 1 cut(s) 524
BsaXI ACNNNNNCTCC 2 cut(s) 1230, 1260
Bsc4I CCNNNNNNNGG 7 cut(s) 50, 262, 378, 459, 603, 1580, 1780
Bse118I RCCGGY 4 cut(s) 153, 255, 262, 1713
Bse1I ACTGG 5 cut(s) 16, 164, 949, 1340, 1477
Bse3DI GCAATG 2 cut(s) 1015, 1248
BseAI TCCGGA 1 cut(s) 524
BseBI CCWGG 2 cut(s) 484, 1861
BseCI ATCGAT 1 cut(s) 928
BseDI CCNNGG 4 cut(s) 375, 589, 735, 959
BseGI GGATG 6 cut(s) 213, 368, 745, 865, 873, 1917
BseLI CCNNNNNNNGG 7 cut(s) 50, 262, 378, 459, 603, 1580, 1780
BseMI GCAATG 2 cut(s) 1015, 1248
BseMII CTCAG 3 cut(s) 1283, 1519, 1914
BseNI ACTGG 5 cut(s) 16, 164, 949, 1340, 1477
BsePI GCGCGC 2 cut(s) 572, 1435
BseXI GCAGC 8 cut(s) 699, 942, 1129, 1307, 1339, 1444, 1615, 1697
BseYI CCCAGC 2 cut(s) 1291, 1299
BsgI GTGCAG 1 cut(s) 1128
Bsh1236I CGCG 6 cut(s) 440, 574, 600, 612, 627, 1437
Bsh1285I CGRYCG 1 cut(s) 334
BshFI GGCC 5 cut(s) 1476, 1640, 1686, 1717, 1859
BshNI GGYRCC 3 cut(s) 593, 606, 716
BshVI ATCGAT 1 cut(s) 928
BsiEI CGRYCG 1 cut(s) 334
BsiHKAI GWGCWC 2 cut(s) 459, 1270
BsiHKCI CYCGRG 3 cut(s) 376, 539, 960
BsiSI CCGG 6 cut(s) 154, 256, 263, 460, 525, 1714
BslFI GGGAC 4 cut(s) 13, 498, 998, 1274
BslI CCNNNNNNNGG 7 cut(s) 50, 262, 378, 459, 603, 1580, 1780
BsmAI GTCTC 4 cut(s) 17, 1710, 1756, 1903
BsmBI CGTCTC 1 cut(s) 1756
BsmFI GGGAC 4 cut(s) 13, 498, 998, 1274
BsnI GGCC 5 cut(s) 1476, 1640, 1686, 1717, 1859
Bso31I GGTCTC 1 cut(s) 17
BsoBI CYCGRG 3 cut(s) 376, 539, 960
Bsp1286I GDGCHC 3 cut(s) 384, 459, 1270
Bsp13I TCCGGA 1 cut(s) 524
Bsp143I GATC 6 cut(s) 331, 925, 929, 1218, 1258, 1869
BspANI GGCC 5 cut(s) 1476, 1640, 1686, 1717, 1859
BspCNI CTCAG 3 cut(s) 1282, 1518, 1913
BspDI ATCGAT 1 cut(s) 928
BspEI TCCGGA 1 cut(s) 524
BspFNI CGCG 6 cut(s) 440, 574, 600, 612, 627, 1437
BspHI TCATGA 2 cut(s) 669, 1555
BspLI GGNNCC 7 cut(s) 383, 514, 595, 608, 718, 778, 1290
BspT107I GGYRCC 3 cut(s) 593, 606, 716
BspTNI GGTCTC 1 cut(s) 17
BsrBI CCGCTC 1 cut(s) 477
BsrDI GCAATG 2 cut(s) 1015, 1248
BsrFI RCCGGY 4 cut(s) 153, 255, 262, 1713
BsrI ACTGG 5 cut(s) 16, 164, 949, 1340, 1477
BssAI RCCGGY 4 cut(s) 153, 255, 262, 1713
BssECI CCNNGG 4 cut(s) 375, 589, 735, 959
BssHII GCGCGC 2 cut(s) 572, 1435
BssMI GATC 6 cut(s) 331, 925, 929, 1218, 1258, 1869
BssNI GRCGYC 3 cut(s) 717, 1765, 1905
BssSI CACGAG 1 cut(s) 1152
Bst2BI CACGAG 1 cut(s) 1152
Bst2UI CCWGG 2 cut(s) 484, 1861
Bst4CI ACNGT 5 cut(s) 145, 1149, 1601, 1779, 1794
Bst6I CTCTTC 2 cut(s) 1209, 1539
BstACI GRCGYC 3 cut(s) 717, 1765, 1905
BstC8I GCNNGC 7 cut(s) 338, 402, 549, 574, 1437, 1575, 1715
BstDEI CTNAG 4 cut(s) 1035, 1269, 1505, 1900
BstDSI CCRYGG 1 cut(s) 589
BstEII GGTNACC 3 cut(s) 91, 265, 442
BstF5I GGATG 6 cut(s) 213, 368, 745, 865, 873, 1917
BstFNI CGCG 6 cut(s) 440, 574, 600, 612, 627, 1437
BstH2I RGCGCY 1 cut(s) 720
BstHHI GCGC 7 cut(s) 489, 574, 576, 627, 719, 1437, 1439
BstKTI GATC 6 cut(s) 334, 928, 932, 1221, 1261, 1872
BstMAI GTCTC 4 cut(s) 17, 1710, 1756, 1903
BstMBI GATC 6 cut(s) 331, 925, 929, 1218, 1258, 1869
BstMCI CGRYCG 1 cut(s) 334
BstMWI GCNNNNNNNGC 9 cut(s) 410, 493, 544, 725, 1175, 1247, 1336, 1406, 1637
BstNI CCWGG 2 cut(s) 484, 1861
BstNSI RCATGY 1 cut(s) 586
BstPI GGTNACC 3 cut(s) 91, 265, 442
BstSCI CCNGG 3 cut(s) 459, 482, 1859
BstSFI CTRYAG 1 cut(s) 1775
BstUI CGCG 6 cut(s) 440, 574, 600, 612, 627, 1437
BstV1I GCAGC 8 cut(s) 699, 942, 1129, 1307, 1339, 1444, 1615, 1697
BstX2I RGATCY 1 cut(s) 1258
BstXI CCANNNNNNTGG 2 cut(s) 673, 1648
BstYI RGATCY 1 cut(s) 1258
Bsu15I ATCGAT 1 cut(s) 928
BsuI GTATCC 1 cut(s) 647
BsuRI GGCC 5 cut(s) 1476, 1640, 1686, 1717, 1859
BsuTUI ATCGAT 1 cut(s) 928
BtgI CCRYGG 1 cut(s) 589
BtgZI GCGATG 1 cut(s) 858
BtsCI GGATG 6 cut(s) 213, 368, 745, 865, 873, 1917
BtsI GCAGTG 2 cut(s) 1431, 1919
BtsIMutI CAGTG 3 cut(s) 1431, 1784, 1919
Cac8I GCNNGC 7 cut(s) 338, 402, 549, 574, 1437, 1575, 1715
CciI TCATGA 2 cut(s) 669, 1555
CfoI GCGC 7 cut(s) 489, 574, 576, 627, 719, 1437, 1439
Cfr10I RCCGGY 4 cut(s) 153, 255, 262, 1713
Cfr13I GGNCC 4 cut(s) 512, 899, 1288, 1474
ClaI ATCGAT 1 cut(s) 928
CseI GACGC 2 cut(s) 446, 616
Csp6I GTAC 4 cut(s) 146, 185, 921, 1757
CviAII CATG 7 cut(s) 410, 562, 583, 670, 1015, 1556, 1690
CviQI GTAC 4 cut(s) 146, 185, 921, 1757
DdeI CTNAG 4 cut(s) 1035, 1269, 1505, 1900
DinI GGCGCC 1 cut(s) 718
DpnI GATC 6 cut(s) 333, 927, 931, 1220, 1260, 1871
DpnII GATC 6 cut(s) 331, 925, 929, 1218, 1258, 1869
DraI TTTAAA 1 cut(s) 117
EaeI YGGCCR 2 cut(s) 1638, 1715
Eam1104I CTCTTC 2 cut(s) 1209, 1539
EarI CTCTTC 2 cut(s) 1209, 1539
EciI GGCGGA 1 cut(s) 715
Ecl136II GAGCTC 1 cut(s) 457
Eco24I GRGCYC 2 cut(s) 384, 459
Eco31I GGTCTC 1 cut(s) 17
Eco47I GGWCC 3 cut(s) 512, 899, 1288
Eco53kI GAGCTC 1 cut(s) 457
Eco57I CTGAAG 3 cut(s) 846, 1440, 1874
Eco88I CYCGRG 3 cut(s) 376, 539, 960
Eco91I GGTNACC 3 cut(s) 91, 265, 442
EcoICRI GAGCTC 1 cut(s) 457
EcoO109I RGGNCCY 1 cut(s) 1288
EcoO65I GGTNACC 3 cut(s) 91, 265, 442
EcoRII CCWGG 2 cut(s) 482, 1859
EcoT38I GRGCYC 2 cut(s) 384, 459
EgeI GGCGCC 1 cut(s) 718
EheI GGCGCC 1 cut(s) 718
Esp3I CGTCTC 1 cut(s) 1756
FaeI CATG 7 cut(s) 413, 565, 586, 673, 1018, 1559, 1693
FaqI GGGAC 4 cut(s) 13, 498, 998, 1274
FatI CATG 7 cut(s) 409, 561, 582, 669, 1014, 1555, 1689
FauI CCCGC 4 cut(s) 105, 523, 605, 857
FauNDI CATATG 1 cut(s) 699
FblI GTMKAC 2 cut(s) 435, 645
Fnu4HI GCNGC 9 cut(s) 559, 688, 956, 1143, 1296, 1328, 1433, 1629, 1711
FokI GGATG 6 cut(s) 200, 355, 752, 872, 880, 1904
FriOI GRGCYC 2 cut(s) 384, 459
Fsp4HI GCNGC 9 cut(s) 559, 688, 956, 1143, 1296, 1328, 1433, 1629, 1711
FspBI CTAG 2 cut(s) 1280, 1395
GlaI GCGC 7 cut(s) 488, 573, 575, 626, 718, 1436, 1438
GluI GCNGC 9 cut(s) 559, 688, 956, 1143, 1296, 1328, 1433, 1629, 1711
GsaI CCCAGC 2 cut(s) 1295, 1303
GsuI CTGGAG 2 cut(s) 403, 1000
HaeII RGCGCY 1 cut(s) 720
HaeIII GGCC 5 cut(s) 1476, 1640, 1686, 1717, 1859
HapII CCGG 6 cut(s) 154, 256, 263, 460, 525, 1714
HgaI GACGC 2 cut(s) 446, 616
HhaI GCGC 7 cut(s) 489, 574, 576, 627, 719, 1437, 1439
Hin1I GRCGYC 3 cut(s) 717, 1765, 1905
Hin1II CATG 7 cut(s) 413, 565, 586, 673, 1018, 1559, 1693
Hin6I GCGC 7 cut(s) 487, 572, 574, 625, 717, 1435, 1437
HinP1I GCGC 7 cut(s) 487, 572, 574, 625, 717, 1435, 1437
HincII GTYRAC 3 cut(s) 159, 436, 646
HindII GTYRAC 3 cut(s) 159, 436, 646
HindIII AAGCTT 1 cut(s) 893
HinfI GANTC 8 cut(s) 471, 528, 542, 694, 1196, 1367, 1463, 1874
HpaII CCGG 6 cut(s) 154, 256, 263, 460, 525, 1714
Hpy166II GTNNAC 5 cut(s) 148, 159, 436, 646, 921
Hpy188III TCNNGA 7 cut(s) 525, 631, 670, 902, 979, 1152, 1556
Hpy8I GTNNAC 5 cut(s) 148, 159, 436, 646, 921
Hpy99I CGWCG 4 cut(s) 440, 557, 647, 665
HpyCH4III ACNGT 5 cut(s) 145, 1149, 1601, 1779, 1794
HpyCH4IV ACGT 5 cut(s) 567, 642, 1552, 1765, 1905
HpyCH4V TGCA 7 cut(s) 504, 1067, 1145, 1178, 1620, 1912, 1917
HpyF10VI GCNNNNNNNGC 9 cut(s) 410, 493, 544, 725, 1175, 1247, 1336, 1406, 1637
HpyF3I CTNAG 4 cut(s) 1035, 1269, 1505, 1900
HpySE526I ACGT 5 cut(s) 567, 642, 1552, 1765, 1905
Hsp92I GRCGYC 3 cut(s) 717, 1765, 1905
Hsp92II CATG 7 cut(s) 413, 565, 586, 673, 1018, 1559, 1693
HspAI GCGC 7 cut(s) 487, 572, 574, 625, 717, 1435, 1437
KasI GGCGCC 1 cut(s) 716
Kpn2I TCCGGA 1 cut(s) 524
KroI GCCGGC 1 cut(s) 1713
KroNI GCCGGC 1 cut(s) 1715
Kzo9I GATC 6 cut(s) 331, 925, 929, 1218, 1258, 1869
LmnI GCTCC 6 cut(s) 387, 428, 462, 482, 782, 1238
Lsp1109I GCAGC 8 cut(s) 699, 942, 1129, 1307, 1339, 1444, 1615, 1697
LweI GCATC 7 cut(s) 585, 1238, 1432, 1440, 1648, 1703, 1926
MaeI CTAG 2 cut(s) 1280, 1395
MaeII ACGT 5 cut(s) 567, 642, 1552, 1765, 1905
MaeIII GTNAC 7 cut(s) 91, 139, 166, 265, 352, 442, 1149
MalI GATC 6 cut(s) 333, 927, 931, 1220, 1260, 1871
MbiI CCGCTC 1 cut(s) 477
MboI GATC 6 cut(s) 331, 925, 929, 1218, 1258, 1869
MboII GAAGA 6 cut(s) 123, 289, 839, 1023, 1226, 1556
MflI RGATCY 1 cut(s) 1258
MhlI GDGCHC 3 cut(s) 384, 459, 1270
MlsI TGGCCA 1 cut(s) 1640
MluCI AATT 4 cut(s) 65, 1026, 1645, 1864
MluNI TGGCCA 1 cut(s) 1640
Mly113I GGCGCC 1 cut(s) 717
MlyI GAGTC 4 cut(s) 551, 1190, 1472, 1883
MmeI TCCRAC 2 cut(s) 228, 1026
Mox20I TGGCCA 1 cut(s) 1640
MroI TCCGGA 1 cut(s) 524
MroNI GCCGGC 1 cut(s) 1713
MroXI GAANNNNTTC 1 cut(s) 470
MscI TGGCCA 1 cut(s) 1640
MseI TTAA 5 cut(s) 116, 909, 1119, 1128, 1833
MslI CAYNNNNRTG 5 cut(s) 566, 581, 671, 702, 803
Msp20I TGGCCA 1 cut(s) 1640
MspI CCGG 6 cut(s) 154, 256, 263, 460, 525, 1714
MspR9I CCNGG 3 cut(s) 461, 484, 1861
MvaI CCWGG 2 cut(s) 484, 1861
MvnI CGCG 6 cut(s) 440, 574, 600, 612, 627, 1437
MwoI GCNNNNNNNGC 9 cut(s) 410, 493, 544, 725, 1175, 1247, 1336, 1406, 1637
NaeI GCCGGC 1 cut(s) 1715
NarI GGCGCC 1 cut(s) 717
NciI CCSGG 1 cut(s) 461
NdeI CATATG 1 cut(s) 699
NdeII GATC 6 cut(s) 331, 925, 929, 1218, 1258, 1869
NgoMIV GCCGGC 1 cut(s) 1713
NlaIII CATG 7 cut(s) 413, 565, 586, 673, 1018, 1559, 1693
NlaIV GGNNCC 7 cut(s) 383, 514, 595, 608, 718, 778, 1290
NmeAIII GCCGAG 2 cut(s) 478, 760
NmuCI GTSAC 6 cut(s) 139, 166, 265, 352, 442, 1149
NspI RCATGY 1 cut(s) 586
PaeR7I CTCGAG 1 cut(s) 539
PagI TCATGA 2 cut(s) 669, 1555
PauI GCGCGC 2 cut(s) 572, 1435
PciI ACATGT 1 cut(s) 582
PcsI WCGNNNNNNNCGW 4 cut(s) 444, 537, 597, 1878
PdiI GCCGGC 1 cut(s) 1715
PdmI GAANNNNTTC 1 cut(s) 470
PfeI GAWTC 4 cut(s) 471, 528, 694, 1367
PflFI GACNNNGTC 1 cut(s) 440
PflMI CCANNNNNTGG 1 cut(s) 1580
PfoI TCCNGGA 1 cut(s) 459
PkrI GCNGC 9 cut(s) 560, 689, 957, 1144, 1297, 1329, 1434, 1630, 1712
Ple19I CGATCG 1 cut(s) 334
PleI GAGTC 4 cut(s) 550, 1190, 1471, 1882
PluTI GGCGCC 1 cut(s) 720
PpsI GAGTC 4 cut(s) 550, 1190, 1471, 1882
PpuMI RGGWCCY 1 cut(s) 1288
PscI ACATGT 1 cut(s) 582
PshBI ATTAAT 1 cut(s) 1119
Psp124BI GAGCTC 1 cut(s) 459
Psp5II RGGWCCY 1 cut(s) 1288
Psp6I CCWGG 2 cut(s) 482, 1859
PspEI GGTNACC 3 cut(s) 91, 265, 442
PspFI CCCAGC 2 cut(s) 1291, 1299
PspGI CCWGG 2 cut(s) 482, 1859
PspN4I GGNNCC 7 cut(s) 383, 514, 595, 608, 718, 778, 1290
PspPI GGNCC 4 cut(s) 512, 899, 1288, 1474
PspPPI RGGWCCY 1 cut(s) 1288
PspXI VCTCGAGB 1 cut(s) 539
PsuI RGATCY 1 cut(s) 1258
PsyI GACNNNGTC 1 cut(s) 440
PteI GCGCGC 2 cut(s) 572, 1435
PvuI CGATCG 1 cut(s) 334
RsaI GTAC 4 cut(s) 147, 186, 922, 1758
RsaNI GTAC 4 cut(s) 146, 185, 921, 1757
RseI CAYNNNNRTG 5 cut(s) 566, 581, 671, 702, 803
SacI GAGCTC 1 cut(s) 459
SalI GTCGAC 2 cut(s) 434, 644
SaqAI TTAA 5 cut(s) 116, 909, 1119, 1128, 1833
SatI GCNGC 9 cut(s) 559, 688, 956, 1143, 1296, 1328, 1433, 1629, 1711
Sau3AI GATC 6 cut(s) 331, 925, 929, 1218, 1258, 1869
Sau96I GGNCC 4 cut(s) 512, 899, 1288, 1474
SchI GAGTC 4 cut(s) 551, 1190, 1472, 1883
ScrFI CCNGG 3 cut(s) 461, 484, 1861
SduI GDGCHC 3 cut(s) 384, 459, 1270
SfaNI GCATC 7 cut(s) 585, 1238, 1432, 1440, 1648, 1703, 1926
SfcI CTRYAG 1 cut(s) 1775
SfoI GGCGCC 1 cut(s) 718
Sfr274I CTCGAG 1 cut(s) 539
SinI GGWCC 3 cut(s) 512, 899, 1288
SlaI CTCGAG 1 cut(s) 539
SmiMI CAYNNNNRTG 5 cut(s) 566, 581, 671, 702, 803
SmlI CTYRAG 1 cut(s) 539
SmoI CTYRAG 1 cut(s) 539
Sse9I AATT 4 cut(s) 65, 1026, 1645, 1864
SspDI GGCGCC 1 cut(s) 716
SspMI CTAG 2 cut(s) 1280, 1395
SstI GAGCTC 1 cut(s) 459
StyD4I CCNGG 3 cut(s) 459, 482, 1859
TaaI ACNGT 5 cut(s) 145, 1149, 1601, 1779, 1794
TaiI ACGT 5 cut(s) 570, 645, 1555, 1768, 1908
TaqI TCGA 8 cut(s) 429, 435, 531, 540, 645, 879, 928, 1872
TaqII GACCGA 1 cut(s) 500
TasI AATT 4 cut(s) 65, 1026, 1645, 1864
TatI WGTACW 1 cut(s) 920
TauI GCSGC 1 cut(s) 561
TfiI GAWTC 4 cut(s) 471, 528, 694, 1367
Tru1I TTAA 5 cut(s) 116, 909, 1119, 1128, 1833
Tru9I TTAA 5 cut(s) 116, 909, 1119, 1128, 1833
TscAI CASTG 3 cut(s) 1431, 1784, 1919
TseFI GTSAC 6 cut(s) 139, 166, 265, 352, 442, 1149
TseI GCWGC 8 cut(s) 687, 955, 1142, 1295, 1327, 1432, 1628, 1710
Tsp45I GTSAC 6 cut(s) 139, 166, 265, 352, 442, 1149
TspDTI ATGAA 6 cut(s) 578, 686, 828, 861, 1572, 1578
TspGWI ACGGA 5 cut(s) 127, 172, 334, 483, 1774
TspRI CASTG 3 cut(s) 1431, 1784, 1919
Tth111I GACNNNGTC 1 cut(s) 440
Van91I CCANNNNNTGG 1 cut(s) 1580
VpaK11BI GGWCC 3 cut(s) 512, 899, 1288
VspI ATTAAT 1 cut(s) 1119
XapI RAATTY 1 cut(s) 1864
XceI RCATGY 1 cut(s) 586
XhoI CTCGAG 1 cut(s) 539
XmiI GTMKAC 2 cut(s) 435, 645
XmnI GAANNNNTTC 1 cut(s) 470
XspI CTAG 2 cut(s) 1280, 1395
ZraI GACGTC 2 cut(s) 1766, 1906
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.