Rmu_sc0000697.1_g000006

Belongs to the pyruvate kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000697.1
OG0009431
Physical Location & Seq
Reverse (-)
68413 .. 72717
4305 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000697.1_g000006.1.cds

Sequence Viewer

Length: 1107 bp
atgcacgattttcactcaaaggaaaatggggctgaaggtgttgatttcattgctgtatcttttgtcaagtctgctgaagtgatcaatcaccttaaaagctatattgcagcacggtctcgcgatatttctgttgtggcaaagatagagagtattgactctctaaagaacttggaagagatcatccttgcatcagatggagcaatggtagcaagaggtgacctaggtgctcagataccattggaacaggtcccatcagcccagcaaaagattgttcaagtatgtcggcagctaaataagccagtcattgttgcttctcaactactcgaatctatgatcgagtatcctacacccactagagctgaagtggccgatgtttctgaagccgtgaggcagcgagctgatgctctgatgctttctggtgagtcagctatgggccagttcccagataagtcattagctgttctcagaagtgtcagtttgaggatcgagaggtggtggagggaagagaaacgtcatgaacctatgttactcccagctattggatcatcattttcagacagtatatcggaggagctttgtatttctgctgccaagatggcaaataatttagaggtggatgctcttttcatctttacaaagactggccacatggcatctctcctgtcgcgctgccggcctgactgcccaatctttgcttttaccaacacaacatctgtgcggagacgtctgaacctacagtgggggctgataccatttcgtgttagtttctctgatgatatggaaagcaaccttcacaagaccttctcactgctgaaggccaggaatctgatcaaatcaggcttctcagaagctagtggcaacaatcattgtggtgctccaacaccttcgactcaaaatgctgtgatcgagcattcgagctgctgcataagtatcatctccatattcggctgccctgacgagctctcagcactttggcactttaaggacggttgcagtgttgcacttccagaaaaaggggcagtggggacgatatcaagtgtattgatcgaacagactaacaggccaagcattaatttccttgtattgtgcttcgagtattttaattaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000287 GO:0003006 GO:0003674 GO:0003824 GO:0004743 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006629 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009791 GO:0009987 GO:0010035 GO:0010038 GO:0010154 GO:0010431 GO:0016052 GO:0016053 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0021700 GO:0022414 GO:0030955 GO:0031420 GO:0032501 GO:0032502 GO:0032504 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042221 GO:0042866 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046686 GO:0046700 GO:0046872 GO:0046939 GO:0048316 GO:0048608 GO:0048609 GO:0048731 GO:0048856 GO:0050896 GO:0051186 GO:0051188 GO:0055086 GO:0061458 GO:0071695 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00230 ko00620 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko04922 ko04930 ko05165 ko05203 ko05230 map00010 map00230 map00620 map01100 map01110 map01120 map01130 map01200 map01230 map04922 map04930 map05165 map05203 map05230
Pfam Domains
Protein Families
PK PK_C

Protein Analysis

368

Amino Acids

40.49

Weight (kDa)

5.89

Isoelectric Point (pI)

45.31

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 727
AccB7I CCANNNNNTGG 1 cut(s) 539
AccII CGCG 2 cut(s) 120, 667
AciI CCGC 1 cut(s) 718
AclWI GGATC 2 cut(s) 491, 550
AcoI YGGCCR 2 cut(s) 366, 643
AcuI CTGAAG 5 cut(s) 54, 96, 381, 399, 833
AcyI GRCGYC 1 cut(s) 724
AfiI CCNNNNNNNGG 3 cut(s) 539, 739, 1013
AgsI TTSAA 1 cut(s) 275
AjnI CCWGG 1 cut(s) 818
Alw21I GWGCWC 3 cut(s) 229, 877, 963
Alw26I GTCTC 2 cut(s) 120, 715
AlwI GGATC 2 cut(s) 491, 550
AoxI GGCC 6 cut(s) 366, 433, 643, 674, 816, 1061
ApeKI GCWGC 8 cut(s) 107, 286, 391, 587, 669, 918, 921, 948
AseI ATTAAT 1 cut(s) 1071
AspA2I CCTAGG 1 cut(s) 220
AspLEI GCGC 1 cut(s) 669
AspS9I GGNCC 2 cut(s) 247, 433
AsuHPI GGTGA 3 cut(s) 80, 227, 431
AvaII GGWCC 1 cut(s) 247
AvrII CCTAGG 1 cut(s) 220
BalI TGGCCA 1 cut(s) 645
BanII GRGCYC 1 cut(s) 963
Bbv12I GWGCWC 3 cut(s) 229, 877, 963
BbvI GCAGC 8 cut(s) 119, 298, 403, 574, 656, 905, 908, 935
BccI CCATC 3 cut(s) 188, 259, 589
BceAI ACGGC 1 cut(s) 368
BciT130I CCWGG 1 cut(s) 820
BciVI GTATCC 1 cut(s) 351
BclI TGATCA 2 cut(s) 81, 828
BcoDI GTCTC 2 cut(s) 120, 715
BfaI CTAG 3 cut(s) 221, 354, 852
BfmI CTRYAG 1 cut(s) 734
BfuI GTATCC 1 cut(s) 351
BglI GCCNNNNNGGC 1 cut(s) 596
BisI GCNGC 8 cut(s) 108, 287, 392, 588, 670, 919, 922, 949
BlnI CCTAGG 1 cut(s) 220
BlsI GCNGC 8 cut(s) 109, 288, 393, 589, 671, 920, 923, 950
Bme1390I CCNGG 1 cut(s) 820
Bme18I GGWCC 1 cut(s) 247
BmgT120I GGNCC 2 cut(s) 247, 433
BmiI GGNNCC 1 cut(s) 249
BmrFI CCNGG 1 cut(s) 820
BmsI GCATC 5 cut(s) 197, 391, 399, 607, 662
BsaHI GRCGYC 1 cut(s) 724
BsaI GGTCTC 1 cut(s) 120
BsaJI CCNNGG 1 cut(s) 220
BsaXI ACNNNNNCTCC 2 cut(s) 189, 219
Bsc4I CCNNNNNNNGG 3 cut(s) 539, 739, 1013
Bse118I RCCGGY 1 cut(s) 672
Bse1I ACTGG 3 cut(s) 299, 436, 646
Bse3DI GCAATG 2 cut(s) 48, 207
BseBI CCWGG 1 cut(s) 820
BseDI CCNNGG 1 cut(s) 220
BseGI GGATG 2 cut(s) 180, 622
BseLI CCNNNNNNNGG 3 cut(s) 539, 739, 1013
BseMI GCAATG 2 cut(s) 48, 207
BseMII CTCAG 4 cut(s) 242, 478, 858, 978
BseNI ACTGG 3 cut(s) 299, 436, 646
BseRI GAGGAG 1 cut(s) 584
BseXI GCAGC 8 cut(s) 119, 298, 403, 574, 656, 905, 908, 935
BseYI CCCAGC 2 cut(s) 258, 532
Bsh1236I CGCG 2 cut(s) 120, 667
BshFI GGCC 6 cut(s) 368, 435, 645, 676, 818, 1063
BsiHKAI GWGCWC 3 cut(s) 229, 877, 963
BsiSI CCGG 1 cut(s) 673
BslFI GGGAC 2 cut(s) 233, 1039
BslI CCNNNNNNNGG 3 cut(s) 539, 739, 1013
BsmAI GTCTC 2 cut(s) 120, 715
BsmBI CGTCTC 1 cut(s) 715
BsmFI GGGAC 2 cut(s) 233, 1039
BsmI GAATGC 1 cut(s) 910
BsnI GGCC 6 cut(s) 368, 435, 645, 676, 818, 1063
Bso31I GGTCTC 1 cut(s) 120
Bsp1286I GDGCHC 3 cut(s) 229, 877, 963
Bsp143I GATC 8 cut(s) 81, 177, 333, 483, 542, 828, 903, 1044
Bsp68I TCGCGA 1 cut(s) 120
BspACI CCGC 1 cut(s) 718
BspANI GGCC 6 cut(s) 368, 435, 645, 676, 818, 1063
BspCNI CTCAG 4 cut(s) 241, 477, 857, 977
BspFNI CGCG 2 cut(s) 120, 667
BspHI TCATGA 1 cut(s) 514
BspLI GGNNCC 1 cut(s) 249
BspPI GGATC 2 cut(s) 491, 550
BspTNI GGTCTC 1 cut(s) 120
BsrDI GCAATG 2 cut(s) 48, 207
BsrFI RCCGGY 1 cut(s) 672
BsrI ACTGG 3 cut(s) 299, 436, 646
BssAI RCCGGY 1 cut(s) 672
BssECI CCNNGG 1 cut(s) 220
BssMI GATC 8 cut(s) 81, 177, 333, 483, 542, 828, 903, 1044
BssNI GRCGYC 1 cut(s) 724
BssT1I CCWWGG 1 cut(s) 220
Bst2UI CCWGG 1 cut(s) 820
Bst4CI ACNGT 4 cut(s) 114, 560, 738, 989
Bst6I CTCTTC 2 cut(s) 168, 498
BstACI GRCGYC 1 cut(s) 724
BstC8I GCNNGC 2 cut(s) 396, 674
BstDEI CTNAG 4 cut(s) 228, 464, 844, 964
BstEII GGTNACC 1 cut(s) 215
BstF5I GGATG 2 cut(s) 180, 622
BstFNI CGCG 2 cut(s) 120, 667
BstHHI GCGC 1 cut(s) 669
BstKTI GATC 8 cut(s) 84, 180, 336, 486, 545, 831, 906, 1047
BstMAI GTCTC 2 cut(s) 120, 715
BstMBI GATC 8 cut(s) 81, 177, 333, 483, 542, 828, 903, 1044
BstMWI GCNNNNNNNGC 5 cut(s) 206, 295, 365, 596, 673
BstNI CCWGG 1 cut(s) 820
BstPI GGTNACC 1 cut(s) 215
BstSCI CCNGG 1 cut(s) 818
BstSFI CTRYAG 1 cut(s) 734
BstUI CGCG 2 cut(s) 120, 667
BstV1I GCAGC 8 cut(s) 119, 298, 403, 574, 656, 905, 908, 935
BsuI GTATCC 1 cut(s) 351
BsuRI GGCC 6 cut(s) 368, 435, 645, 676, 818, 1063
BtsCI GGATG 2 cut(s) 180, 622
BtsI GCAGTG 3 cut(s) 806, 1000, 1026
BtsIMutI CAGTG 4 cut(s) 743, 806, 1000, 1026
BtuMI TCGCGA 1 cut(s) 120
Cac8I GCNNGC 2 cut(s) 396, 674
CciI TCATGA 1 cut(s) 514
CfoI GCGC 1 cut(s) 669
Cfr10I RCCGGY 1 cut(s) 672
Cfr13I GGNCC 2 cut(s) 247, 433
CspCI CAANNNNNGTGG 2 cut(s) 850, 885
CviAII CATG 2 cut(s) 515, 649
DdeI CTNAG 4 cut(s) 228, 464, 844, 964
DpnI GATC 8 cut(s) 83, 179, 335, 485, 544, 830, 905, 1046
DpnII GATC 8 cut(s) 81, 177, 333, 483, 542, 828, 903, 1044
EaeI YGGCCR 2 cut(s) 366, 643
Eam1104I CTCTTC 2 cut(s) 168, 498
EarI CTCTTC 2 cut(s) 168, 498
Ecl136II GAGCTC 1 cut(s) 961
Eco130I CCWWGG 1 cut(s) 220
Eco24I GRGCYC 1 cut(s) 963
Eco31I GGTCTC 1 cut(s) 120
Eco32I GATATC 1 cut(s) 1032
Eco47I GGWCC 1 cut(s) 247
Eco53kI GAGCTC 1 cut(s) 961
Eco57I CTGAAG 5 cut(s) 54, 96, 381, 399, 833
Eco91I GGTNACC 1 cut(s) 215
EcoICRI GAGCTC 1 cut(s) 961
EcoO109I RGGNCCY 1 cut(s) 247
EcoO65I GGTNACC 1 cut(s) 215
EcoRII CCWGG 1 cut(s) 818
EcoRV GATATC 1 cut(s) 1032
EcoT14I CCWWGG 1 cut(s) 220
EcoT38I GRGCYC 1 cut(s) 963
ErhI CCWWGG 1 cut(s) 220
Esp3I CGTCTC 1 cut(s) 715
FaeI CATG 2 cut(s) 518, 652
FaqI GGGAC 2 cut(s) 233, 1039
FatI CATG 2 cut(s) 514, 648
FbaI TGATCA 2 cut(s) 81, 828
Fnu4HI GCNGC 8 cut(s) 108, 287, 392, 588, 670, 919, 922, 949
FokI GGATG 2 cut(s) 167, 629
FriOI GRGCYC 1 cut(s) 963
Fsp4HI GCNGC 8 cut(s) 108, 287, 392, 588, 670, 919, 922, 949
FspBI CTAG 3 cut(s) 221, 354, 852
GlaI GCGC 1 cut(s) 668
GluI GCNGC 8 cut(s) 108, 287, 392, 588, 670, 919, 922, 949
GsaI CCCAGC 2 cut(s) 262, 536
HaeIII GGCC 6 cut(s) 368, 435, 645, 676, 818, 1063
HapII CCGG 1 cut(s) 673
HhaI GCGC 1 cut(s) 669
Hin1I GRCGYC 1 cut(s) 724
Hin1II CATG 2 cut(s) 518, 652
Hin6I GCGC 1 cut(s) 667
HinP1I GCGC 1 cut(s) 667
HinfI GANTC 5 cut(s) 155, 326, 422, 823, 889
HpaII CCGG 1 cut(s) 673
HphI GGTGA 3 cut(s) 80, 227, 431
Hpy188III TCNNGA 4 cut(s) 119, 487, 515, 1007
HpyAV CCTTC 5 cut(s) 29, 800, 808, 811, 894
HpyCH4III ACNGT 4 cut(s) 114, 560, 738, 989
HpyCH4IV ACGT 2 cut(s) 511, 724
HpyCH4V TGCA 6 cut(s) 4, 107, 188, 924, 993, 1001
HpyF10VI GCNNNNNNNGC 5 cut(s) 206, 295, 365, 596, 673
HpyF3I CTNAG 4 cut(s) 228, 464, 844, 964
HpySE526I ACGT 2 cut(s) 511, 724
Hsp92I GRCGYC 1 cut(s) 724
Hsp92II CATG 2 cut(s) 518, 652
HspAI GCGC 1 cut(s) 667
KroI GCCGGC 1 cut(s) 672
KroNI GCCGGC 1 cut(s) 674
Ksp22I TGATCA 2 cut(s) 81, 828
Kzo9I GATC 8 cut(s) 81, 177, 333, 483, 542, 828, 903, 1044
LmnI GCTCC 3 cut(s) 197, 571, 880
Lsp1109I GCAGC 8 cut(s) 119, 298, 403, 574, 656, 905, 908, 935
LweI GCATC 5 cut(s) 197, 391, 399, 607, 662
MaeI CTAG 3 cut(s) 221, 354, 852
MaeII ACGT 2 cut(s) 511, 724
MaeIII GTNAC 2 cut(s) 215, 525
MalI GATC 8 cut(s) 83, 179, 335, 485, 544, 830, 905, 1046
MboI GATC 8 cut(s) 81, 177, 333, 483, 542, 828, 903, 1044
MboII GAAGA 2 cut(s) 185, 515
MhlI GDGCHC 3 cut(s) 229, 877, 963
MlsI TGGCCA 1 cut(s) 645
MluCI AATT 3 cut(s) 604, 1072, 1102
MluNI TGGCCA 1 cut(s) 645
MlyI GAGTC 3 cut(s) 149, 431, 883
MmeI TCCRAC 1 cut(s) 902
MnlI CCTC 7 cut(s) 206, 381, 474, 483, 492, 562, 604
Mox20I TGGCCA 1 cut(s) 645
MroNI GCCGGC 1 cut(s) 672
MscI TGGCCA 1 cut(s) 645
MseI TTAA 5 cut(s) 93, 981, 1071, 1101, 1105
MslI CAYNNNNRTG 1 cut(s) 870
Msp20I TGGCCA 1 cut(s) 645
MspI CCGG 1 cut(s) 673
MspR9I CCNGG 1 cut(s) 820
Mva1269I GAATGC 1 cut(s) 910
MvaI CCWGG 1 cut(s) 820
MvnI CGCG 2 cut(s) 120, 667
MwoI GCNNNNNNNGC 5 cut(s) 206, 295, 365, 596, 673
NaeI GCCGGC 1 cut(s) 674
NdeII GATC 8 cut(s) 81, 177, 333, 483, 542, 828, 903, 1044
NgoMIV GCCGGC 1 cut(s) 672
NlaIII CATG 2 cut(s) 518, 652
NlaIV GGNNCC 1 cut(s) 249
NmuCI GTSAC 1 cut(s) 215
NruI TCGCGA 1 cut(s) 120
PacI TTAATTAA 1 cut(s) 1105
PagI TCATGA 1 cut(s) 514
PctI GAATGC 1 cut(s) 910
PdiI GCCGGC 1 cut(s) 674
PfeI GAWTC 2 cut(s) 326, 823
PflMI CCANNNNNTGG 1 cut(s) 539
PkrI GCNGC 8 cut(s) 109, 288, 393, 589, 671, 920, 923, 950
PleI GAGTC 3 cut(s) 149, 430, 883
PpsI GAGTC 3 cut(s) 149, 430, 883
PpuMI RGGWCCY 1 cut(s) 247
PshBI ATTAAT 1 cut(s) 1071
Psp124BI GAGCTC 1 cut(s) 963
Psp5II RGGWCCY 1 cut(s) 247
Psp6I CCWGG 1 cut(s) 818
PspEI GGTNACC 1 cut(s) 215
PspFI CCCAGC 2 cut(s) 258, 532
PspGI CCWGG 1 cut(s) 818
PspN4I GGNNCC 1 cut(s) 249
PspPI GGNCC 2 cut(s) 247, 433
PspPPI RGGWCCY 1 cut(s) 247
PsrI GAACNNNNNNTAC 2 cut(s) 510, 542
RruI TCGCGA 1 cut(s) 120
RseI CAYNNNNRTG 1 cut(s) 870
SacI GAGCTC 1 cut(s) 963
SaqAI TTAA 5 cut(s) 93, 981, 1071, 1101, 1105
SatI GCNGC 8 cut(s) 108, 287, 392, 588, 670, 919, 922, 949
Sau3AI GATC 8 cut(s) 81, 177, 333, 483, 542, 828, 903, 1044
Sau96I GGNCC 2 cut(s) 247, 433
SchI GAGTC 3 cut(s) 149, 431, 883
ScrFI CCNGG 1 cut(s) 820
SduI GDGCHC 3 cut(s) 229, 877, 963
SfaNI GCATC 5 cut(s) 197, 391, 399, 607, 662
SfcI CTRYAG 1 cut(s) 734
SinI GGWCC 1 cut(s) 247
SmiMI CAYNNNNRTG 1 cut(s) 870
Sse9I AATT 3 cut(s) 604, 1072, 1102
SsiI CCGC 1 cut(s) 718
SspMI CTAG 3 cut(s) 221, 354, 852
SstI GAGCTC 1 cut(s) 963
StyD4I CCNGG 1 cut(s) 818
StyI CCWWGG 1 cut(s) 220
TaaI ACNGT 4 cut(s) 114, 560, 738, 989
TaiI ACGT 2 cut(s) 514, 727
TaqI TCGA 8 cut(s) 324, 336, 486, 887, 906, 914, 1047, 1092
TasI AATT 3 cut(s) 604, 1072, 1102
TfiI GAWTC 2 cut(s) 326, 823
Tru1I TTAA 5 cut(s) 93, 981, 1071, 1101, 1105
Tru9I TTAA 5 cut(s) 93, 981, 1071, 1101, 1105
TscAI CASTG 4 cut(s) 743, 813, 1000, 1026
TseFI GTSAC 1 cut(s) 215
TseI GCWGC 8 cut(s) 107, 286, 391, 587, 669, 918, 921, 948
Tsp45I GTSAC 1 cut(s) 215
TspDTI ATGAA 3 cut(s) 37, 531, 616
TspRI CASTG 4 cut(s) 743, 813, 1000, 1026
Van91I CCANNNNNTGG 1 cut(s) 539
VpaK11BI GGWCC 1 cut(s) 247
VspI ATTAAT 1 cut(s) 1071
XmaJI CCTAGG 1 cut(s) 220
XspI CTAG 3 cut(s) 221, 354, 852
ZraI GACGTC 1 cut(s) 725
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.