FvH4_4g01820

Belongs to the pyruvate kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0009431
Physical Location & Seq
Forward (+)
1585623 .. 1594685
9063 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g01820.t1

Sequence Viewer

Length: 1722 bp
ATGTCTCACTCCCTCCACCTCCTCACCCCAACCGCCTTAACCTTCCCCAATCCCAAACCCCTCTCCTCCTCCGTATTCCTCGCCCCCTTCTCCGCTCCCAACCGCCGCTTTACCTTCCCCGCCCTCTCCGTCAAAGCCTCCGCCGCCGGTTCCGATCAAACCCCCTCCGTCCTCGTCTCCGCCAACGGCACCTCCTCCTCCGTCCTCTCCCCTTCTCCGGACCACGCCTCCGCCACCTCCATCGAGGTCGACGCCGTCACCGAGGCCGAGCTCAGGGAGAATGGATTCCGCAGCACGCGCCGCACCAAGCTCATCTGCACCATCGGCCCCGCCTCCGCAGGGTCCGACCAGCTCGAGGCGCTCGCCGCCGGCGGCATGAACGTCGCGCGCCTCAACATGTGCCACGGCACGCGCGACTGGCACCGCGACGTCATCCAGCGCGTGAGGAAGTTGAATGAGGACAAGGGGTATGCTGTTGCGATTATGATGGATACTGAGGGGAGTGAGATTCACATGGGCGATCTCGGCGGCGCTTCTTCCGTCAAGGCCGAGGATGGTGAGATATGGACTTTTAGTGTCAGAGCTTTCGGTTCTACTCTTCCGGAGCGCACCATCAATGTCAACTATGAAGGTTTCGCTGAAGATGTGAAAGTGGGAGATGAACTACTTGTGGACGGTGGAATGGTGAGGTTTGAGGTGGTTGAGAAGCTTGGCCCTGATGTCAAGTGTAAGTGTACTGATCCCGGACTGTTGCTGCCCCGGGCTAACTTGACTTTCTGGAGGGACGGGAGTCTAGTGCGAGAGCGAAATTCCATGCTTCCCACGATTTCTTCCAAGGATTGGTTGGACATTGATTTCGGGATCGCAGAAGGTGTTGATTTCATTGCTGTATCTTTTGTCAAGTCTGCTGAAGTGATCAATCATCTTAAAAGCTATATTGCAGCACGGTCCAGCGATATTTCTGTTGTGGCAAAGATAGAGAGTATTGACTCTCTAAAGAACTTGGAAGAGATCATCCTTGCATCAGATGGAGCAATGGTAGCAAGAGGTGACCTAGGTGCTCAGATACCATTGGAACAGGTCCCGTCAGCCCAGCAAAAGATTGTTCAAGTATGTCGGCAGCTAAATAAGCCAGTCATTGTTGCTTCTCAATTACTCGAATCTATGATCGAGTATCCTACTCCCACTAGAGCTGAAGTAGCTGATGTTTCTGAAGCAGTGAGGCAGCGAGCTGATGCTCTGATGCTTTCTGGTGAGTCAGCTATGGGCCAGTTCCCAGAGAAGTCATTAGCTGTTCTCAGAAGTGTTAGTTTGAGGATCGAGAGGTGGTGGAGGGAAGAGAAACGTCATGAACCTATGTTACTCCCAGCTGTTGGATCATCATTTTCAGATAGTATATCGGAGGAGCTTTGTATTTCTGCTGCCAAGATGGAAGTGGATGCTCTTTTCATCTTTACAAAGACAGGCCACATGGCATCTCTCCTGTCGCGCTGCAGGCCTGATTGCCCAATCTTTGCTTTCACTAACACAACATCTGTACGGAGACGTTTAAACCTACAGTGGGGGCTGATACCATTTCGTGTGACTTTTTCTGATGATATGGAAAGCAACCTTCACAAGACCTTCTCACTGCTGAAGGCCAGGAATCTGATCAAATCAGGTGACCTTGTCATTGCTGTCTCAGACATATTGCAGTCCATTCAAGTTATGAATGTTCCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000287 GO:0003006 GO:0003674 GO:0003824 GO:0004743 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006629 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009791 GO:0009987 GO:0010035 GO:0010038 GO:0010154 GO:0010431 GO:0016052 GO:0016053 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0021700 GO:0022414 GO:0030955 GO:0031420 GO:0032501 GO:0032502 GO:0032504 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042221 GO:0042866 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046686 GO:0046700 GO:0046872 GO:0046939 GO:0048316 GO:0048608 GO:0048609 GO:0048731 GO:0048856 GO:0050896 GO:0051186 GO:0051188 GO:0055086 GO:0061458 GO:0071695 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00230 ko00620 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko04922 ko04930 ko05165 ko05203 ko05230 map00010 map00230 map00620 map01100 map01110 map01120 map01130 map01200 map01230 map04922 map04930 map05165 map05203 map05230
Pfam Domains
Protein Families
PK PK_C

Protein Analysis

574

Amino Acids

62.49

Weight (kDa)

5.63

Isoelectric Point (pI)

45.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK PF00224 100 - 429 8.6e-92 Pyruvate kinase, barrel domain
PK_C PF02887 464 - 562 1.5e-21 Pyruvate kinase, alpha/beta domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 432
AccB1I GGYRCC 2 cut(s) 188, 420
AccB7I CCANNNNNTGG 2 cut(s) 840, 1373
AccBSI CCGCTC 1 cut(s) 95
AccI GTMKAC 1 cut(s) 249
AccII CGCG 8 cut(s) 298, 386, 388, 412, 414, 426, 441, 1489
AccIII TCCGGA 2 cut(s) 217, 601
AclWI GGATC 4 cut(s) 734, 869, 1325, 1384
AcsI RAATTY 1 cut(s) 808
AcuI CTGAAG 5 cut(s) 660, 930, 1215, 1233, 1655
AcyI GRCGYC 2 cut(s) 252, 429
AfaI GTAC 2 cut(s) 736, 1539
AfiI CCNNNNNNNGG 7 cut(s) 217, 273, 339, 355, 840, 1373, 1561
AflIII ACRYGT 1 cut(s) 396
AgsI TTSAA 3 cut(s) 454, 1109, 1703
AjnI CCWGG 1 cut(s) 1640
Alw21I GWGCWC 2 cut(s) 273, 1063
Alw26I GTCTC 4 cut(s) 9, 181, 1537, 1684
AlwI GGATC 4 cut(s) 734, 869, 1325, 1384
Ama87I CYCGRG 2 cut(s) 353, 759
Aor13HI TCCGGA 2 cut(s) 217, 601
AoxI GGCC 8 cut(s) 264, 325, 546, 712, 1267, 1465, 1496, 1638
ApeKI GCWGC 7 cut(s) 291, 754, 941, 1120, 1225, 1421, 1491
ApoI RAATTY 1 cut(s) 808
ArsI GACNNNNNNTTYG 2 cut(s) 839, 871
Asp700I GAANNNNTTC 1 cut(s) 284
AspA2I CCTAGG 1 cut(s) 1054
AspLEI GCGC 9 cut(s) 300, 361, 388, 390, 414, 441, 533, 609, 1491
AspS9I GGNCC 7 cut(s) 220, 326, 342, 713, 948, 1081, 1267
AsuC2I CCSGG 3 cut(s) 744, 760, 761
AsuHPI GGTGA 7 cut(s) 16, 250, 569, 697, 1061, 1265, 1673
AvaI CYCGRG 2 cut(s) 353, 759
AvaII GGWCC 4 cut(s) 220, 342, 948, 1081
AvrII CCTAGG 1 cut(s) 1054
BanI GGYRCC 2 cut(s) 188, 420
BanII GRGCYC 1 cut(s) 273
Bbv12I GWGCWC 2 cut(s) 273, 1063
BbvI GCAGC 7 cut(s) 303, 741, 953, 1132, 1237, 1408, 1478
BccI CCATC 7 cut(s) 248, 329, 481, 548, 620, 1022, 1423
BceAI ACGGC 3 cut(s) 202, 239, 421
BcgI CGANNNNNNTGC 2 cut(s) 364, 398
BciT130I CCWGG 1 cut(s) 1642
BciVI GTATCC 2 cut(s) 484, 1185
BclI TGATCA 2 cut(s) 915, 1650
BcnI CCSGG 3 cut(s) 744, 760, 761
BcoDI GTCTC 4 cut(s) 9, 181, 1537, 1684
BfaI CTAG 3 cut(s) 794, 1055, 1188
BfmI CTRYAG 2 cut(s) 1492, 1556
BfoI RGCGCY 2 cut(s) 362, 534
BfuI GTATCC 2 cut(s) 484, 1185
BlnI CCTAGG 1 cut(s) 1054
Bme1390I CCNGG 4 cut(s) 744, 760, 761, 1642
Bme18I GGWCC 4 cut(s) 220, 342, 948, 1081
BmeT110I CYCGRG 2 cut(s) 353, 759
BmgT120I GGNCC 7 cut(s) 220, 326, 342, 713, 948, 1081, 1267
BmiI GGNNCC 6 cut(s) 151, 190, 328, 343, 422, 1083
BmrFI CCNGG 4 cut(s) 744, 760, 761, 1642
BmsI GCATC 5 cut(s) 1031, 1225, 1233, 1429, 1484
BoxI GACNNNNGTC 1 cut(s) 789
BpmI CTGGAG 1 cut(s) 799
Bpu10I CCTNAGC 1 cut(s) 272
BpuMI CCSGG 3 cut(s) 744, 760, 761
BsaHI GRCGYC 2 cut(s) 252, 429
BsaJI CCNNGG 7 cut(s) 261, 403, 549, 758, 759, 834, 1054
BsaWI WCCGGW 2 cut(s) 217, 601
BsaXI ACNNNNNCTCC 6 cut(s) 176, 206, 212, 242, 1023, 1053
Bsc4I CCNNNNNNNGG 7 cut(s) 217, 273, 339, 355, 840, 1373, 1561
Bse118I RCCGGY 2 cut(s) 146, 368
Bse1I ACTGG 3 cut(s) 422, 1133, 1270
Bse3DI GCAATG 3 cut(s) 882, 1041, 1671
BseAI TCCGGA 2 cut(s) 217, 601
BseBI CCWGG 1 cut(s) 1642
BseDI CCNNGG 7 cut(s) 261, 403, 549, 758, 759, 834, 1054
BseGI GGATG 4 cut(s) 432, 559, 1014, 1444
BseLI CCNNNNNNNGG 7 cut(s) 217, 273, 339, 355, 840, 1373, 1561
BseMI GCAATG 3 cut(s) 882, 1041, 1671
BseMII CTCAG 5 cut(s) 286, 486, 1076, 1312, 1695
BseNI ACTGG 3 cut(s) 422, 1133, 1270
BsePI GCGCGC 1 cut(s) 386
BseRI GAGGAG 6 cut(s) 11, 55, 58, 184, 187, 1418
BseXI GCAGC 7 cut(s) 303, 741, 953, 1132, 1237, 1408, 1478
BseYI CCCAGC 2 cut(s) 1092, 1366
BsgI GTGCAG 1 cut(s) 301
Bsh1236I CGCG 8 cut(s) 298, 386, 388, 412, 414, 426, 441, 1489
BshFI GGCC 8 cut(s) 266, 327, 548, 714, 1269, 1467, 1498, 1640
BshNI GGYRCC 2 cut(s) 188, 420
BsiHKAI GWGCWC 2 cut(s) 273, 1063
BsiHKCI CYCGRG 2 cut(s) 353, 759
BsiSI CCGG 6 cut(s) 147, 218, 369, 602, 744, 760
BslFI GGGAC 2 cut(s) 797, 1067
BslI CCNNNNNNNGG 7 cut(s) 217, 273, 339, 355, 840, 1373, 1561
BsmAI GTCTC 4 cut(s) 9, 181, 1537, 1684
BsmBI CGTCTC 2 cut(s) 181, 1537
BsmFI GGGAC 2 cut(s) 797, 1067
BsnI GGCC 8 cut(s) 266, 327, 548, 714, 1269, 1467, 1498, 1640
BsoBI CYCGRG 2 cut(s) 353, 759
Bsp1286I GDGCHC 2 cut(s) 273, 1063
Bsp13I TCCGGA 2 cut(s) 217, 601
BspANI GGCC 8 cut(s) 266, 327, 548, 714, 1269, 1467, 1498, 1640
BspCNI CTCAG 5 cut(s) 285, 487, 1075, 1311, 1694
BspEI TCCGGA 2 cut(s) 217, 601
BspFNI CGCG 8 cut(s) 298, 386, 388, 412, 414, 426, 441, 1489
BspHI TCATGA 1 cut(s) 1348
BspLI GGNNCC 6 cut(s) 151, 190, 328, 343, 422, 1083
BspMAI CTGCAG 1 cut(s) 1496
BspPI GGATC 4 cut(s) 734, 869, 1325, 1384
BspT107I GGYRCC 2 cut(s) 188, 420
BsrBI CCGCTC 1 cut(s) 95
BsrDI GCAATG 3 cut(s) 882, 1041, 1671
BsrFI RCCGGY 2 cut(s) 146, 368
BsrI ACTGG 3 cut(s) 422, 1133, 1270
BssAI RCCGGY 2 cut(s) 146, 368
BssECI CCNNGG 7 cut(s) 261, 403, 549, 758, 759, 834, 1054
BssHII GCGCGC 1 cut(s) 386
BssNI GRCGYC 2 cut(s) 252, 429
BssT1I CCWWGG 2 cut(s) 834, 1054
Bst2UI CCWGG 1 cut(s) 1642
Bst4CI ACNGT 4 cut(s) 677, 750, 948, 1560
Bst6I CTCTTC 3 cut(s) 603, 1002, 1332
BstACI GRCGYC 2 cut(s) 252, 429
BstC8I GCNNGC 7 cut(s) 296, 363, 370, 388, 410, 1230, 1496
BstDEI CTNAG 5 cut(s) 272, 495, 1062, 1298, 1681
BstDSI CCRYGG 1 cut(s) 403
BstEII GGTNACC 2 cut(s) 1049, 1661
BstF5I GGATG 4 cut(s) 432, 559, 1014, 1444
BstFNI CGCG 8 cut(s) 298, 386, 388, 412, 414, 426, 441, 1489
BstH2I RGCGCY 2 cut(s) 362, 534
BstHHI GCGC 9 cut(s) 300, 361, 388, 390, 414, 441, 533, 609, 1491
BstMAI GTCTC 4 cut(s) 9, 181, 1537, 1684
BstNI CCWGG 1 cut(s) 1642
BstNSI RCATGY 1 cut(s) 400
BstPAI GACNNNNGTC 1 cut(s) 789
BstPI GGTNACC 2 cut(s) 1049, 1661
BstSCI CCNGG 4 cut(s) 742, 758, 759, 1640
BstSFI CTRYAG 2 cut(s) 1492, 1556
BstUI CGCG 8 cut(s) 298, 386, 388, 412, 414, 426, 441, 1489
BstV1I GCAGC 7 cut(s) 303, 741, 953, 1132, 1237, 1408, 1478
BsuI GTATCC 2 cut(s) 484, 1185
BsuRI GGCC 8 cut(s) 266, 327, 548, 714, 1269, 1467, 1498, 1640
BtgI CCRYGG 1 cut(s) 403
BtsCI GGATG 4 cut(s) 432, 559, 1014, 1444
BtsI GCAGTG 2 cut(s) 1224, 1628
BtsIMutI CAGTG 3 cut(s) 1224, 1565, 1628
Cac8I GCNNGC 7 cut(s) 296, 363, 370, 388, 410, 1230, 1496
CciI TCATGA 1 cut(s) 1348
CfoI GCGC 9 cut(s) 300, 361, 388, 390, 414, 441, 533, 609, 1491
Cfr10I RCCGGY 2 cut(s) 146, 368
Cfr13I GGNCC 7 cut(s) 220, 326, 342, 713, 948, 1081, 1267
Cfr9I CCCGGG 1 cut(s) 759
CseI GACGC 1 cut(s) 260
Csp6I GTAC 2 cut(s) 735, 1538
CviAII CATG 6 cut(s) 376, 397, 514, 814, 1349, 1471
CviQI GTAC 2 cut(s) 735, 1538
DdeI CTNAG 5 cut(s) 272, 495, 1062, 1298, 1681
DraI TTTAAA 1 cut(s) 1551
Eam1104I CTCTTC 3 cut(s) 603, 1002, 1332
EarI CTCTTC 3 cut(s) 603, 1002, 1332
EciI GGCGGA 3 cut(s) 130, 169, 220
Ecl136II GAGCTC 1 cut(s) 271
Eco130I CCWWGG 2 cut(s) 834, 1054
Eco147I AGGCCT 1 cut(s) 1498
Eco24I GRGCYC 1 cut(s) 273
Eco47I GGWCC 4 cut(s) 220, 342, 948, 1081
Eco53kI GAGCTC 1 cut(s) 271
Eco57I CTGAAG 5 cut(s) 660, 930, 1215, 1233, 1655
Eco88I CYCGRG 2 cut(s) 353, 759
Eco91I GGTNACC 2 cut(s) 1049, 1661
EcoICRI GAGCTC 1 cut(s) 271
EcoO109I RGGNCCY 1 cut(s) 1081
EcoO65I GGTNACC 2 cut(s) 1049, 1661
EcoRII CCWGG 1 cut(s) 1640
EcoT14I CCWWGG 2 cut(s) 834, 1054
EcoT38I GRGCYC 1 cut(s) 273
ErhI CCWWGG 2 cut(s) 834, 1054
Esp3I CGTCTC 2 cut(s) 181, 1537
FaeI CATG 6 cut(s) 379, 400, 517, 817, 1352, 1474
FaqI GGGAC 2 cut(s) 797, 1067
FatI CATG 6 cut(s) 375, 396, 513, 813, 1348, 1470
FauI CCCGC 2 cut(s) 127, 337
FbaI TGATCA 2 cut(s) 915, 1650
FblI GTMKAC 1 cut(s) 249
FokI GGATG 4 cut(s) 419, 566, 1001, 1451
FriOI GRGCYC 1 cut(s) 273
FspBI CTAG 3 cut(s) 794, 1055, 1188
GlaI GCGC 9 cut(s) 299, 360, 387, 389, 413, 440, 532, 608, 1490
GsaI CCCAGC 2 cut(s) 1096, 1370
GsuI CTGGAG 1 cut(s) 799
HaeII RGCGCY 2 cut(s) 362, 534
HaeIII GGCC 8 cut(s) 266, 327, 548, 714, 1269, 1467, 1498, 1640
HapII CCGG 6 cut(s) 147, 218, 369, 602, 744, 760
HgaI GACGC 1 cut(s) 260
HhaI GCGC 9 cut(s) 300, 361, 388, 390, 414, 441, 533, 609, 1491
Hin1I GRCGYC 2 cut(s) 252, 429
Hin1II CATG 6 cut(s) 379, 400, 517, 817, 1352, 1474
Hin6I GCGC 9 cut(s) 298, 359, 386, 388, 412, 439, 531, 607, 1489
HinP1I GCGC 9 cut(s) 298, 359, 386, 388, 412, 439, 531, 607, 1489
HincII GTYRAC 2 cut(s) 250, 622
HindII GTYRAC 2 cut(s) 250, 622
HindIII AAGCTT 1 cut(s) 707
HinfI GANTC 7 cut(s) 285, 508, 790, 989, 1160, 1256, 1645
HpaII CCGG 6 cut(s) 147, 218, 369, 602, 744, 760
HphI GGTGA 7 cut(s) 16, 250, 569, 697, 1061, 1265, 1673
Hpy166II GTNNAC 4 cut(s) 250, 622, 673, 735
Hpy188III TCNNGA 6 cut(s) 218, 602, 778, 859, 1321, 1349
Hpy8I GTNNAC 4 cut(s) 250, 622, 673, 735
Hpy99I CGWCG 3 cut(s) 254, 386, 431
HpyAV CCTTC 9 cut(s) 52, 97, 124, 222, 623, 863, 1622, 1630, 1633
HpyCH4III ACNGT 4 cut(s) 677, 750, 948, 1560
HpyCH4IV ACGT 4 cut(s) 381, 429, 1345, 1546
HpyCH4V TGCA 5 cut(s) 318, 941, 1022, 1494, 1693
HpyF3I CTNAG 5 cut(s) 272, 495, 1062, 1298, 1681
HpySE526I ACGT 4 cut(s) 381, 429, 1345, 1546
Hsp92I GRCGYC 2 cut(s) 252, 429
Hsp92II CATG 6 cut(s) 379, 400, 517, 817, 1352, 1474
HspAI GCGC 9 cut(s) 298, 359, 386, 388, 412, 439, 531, 607, 1489
Kpn2I TCCGGA 2 cut(s) 217, 601
KroI GCCGGC 1 cut(s) 368
KroNI GCCGGC 1 cut(s) 370
Ksp22I TGATCA 2 cut(s) 915, 1650
LmnI GCTCC 4 cut(s) 100, 604, 1031, 1405
Lsp1109I GCAGC 7 cut(s) 303, 741, 953, 1132, 1237, 1408, 1478
LweI GCATC 5 cut(s) 1031, 1225, 1233, 1429, 1484
MaeI CTAG 3 cut(s) 794, 1055, 1188
MaeII ACGT 4 cut(s) 381, 429, 1345, 1546
MaeIII GTNAC 5 cut(s) 256, 1049, 1359, 1582, 1661
MbiI CCGCTC 1 cut(s) 95
MboII GAAGA 6 cut(s) 528, 590, 653, 822, 1019, 1349
MhlI GDGCHC 2 cut(s) 273, 1063
MluCI AATT 2 cut(s) 808, 1151
MlyI GAGTC 3 cut(s) 799, 983, 1265
MmeI TCCRAC 3 cut(s) 369, 825, 1354
MreI CGCCGGCG 1 cut(s) 368
MroI TCCGGA 2 cut(s) 217, 601
MroNI GCCGGC 1 cut(s) 368
MroXI GAANNNNTTC 1 cut(s) 284
MseI TTAA 3 cut(s) 38, 927, 1550
MspA1I CMGCKG 1 cut(s) 1370
MspI CCGG 6 cut(s) 147, 218, 369, 602, 744, 760
MspR9I CCNGG 4 cut(s) 744, 760, 761, 1642
MssI GTTTAAAC 1 cut(s) 1551
MvaI CCWGG 1 cut(s) 1642
MvnI CGCG 8 cut(s) 298, 386, 388, 412, 414, 426, 441, 1489
NaeI GCCGGC 1 cut(s) 370
NciI CCSGG 3 cut(s) 744, 760, 761
NgoMIV GCCGGC 1 cut(s) 368
NlaIII CATG 6 cut(s) 379, 400, 517, 817, 1352, 1474
NlaIV GGNNCC 6 cut(s) 151, 190, 328, 343, 422, 1083
NmeAIII GCCGAG 3 cut(s) 292, 504, 574
NmuCI GTSAC 4 cut(s) 256, 1049, 1582, 1661
NspI RCATGY 1 cut(s) 400
PaeR7I CTCGAG 1 cut(s) 353
PagI TCATGA 1 cut(s) 1348
PauI GCGCGC 1 cut(s) 386
PceI AGGCCT 1 cut(s) 1498
PciI ACATGT 1 cut(s) 396
PcsI WCGNNNNNNNCGW 2 cut(s) 258, 411
PdiI GCCGGC 1 cut(s) 370
PdmI GAANNNNTTC 1 cut(s) 284
PfeI GAWTC 4 cut(s) 285, 508, 1160, 1645
PflFI GACNNNGTC 2 cut(s) 254, 1667
PflMI CCANNNNNTGG 2 cut(s) 840, 1373
PfoI TCCNGGA 1 cut(s) 742
PleI GAGTC 3 cut(s) 798, 983, 1264
PmeI GTTTAAAC 1 cut(s) 1551
PpsI GAGTC 3 cut(s) 798, 983, 1264
PpuMI RGGWCCY 1 cut(s) 1081
PscI ACATGT 1 cut(s) 396
PshAI GACNNNNGTC 1 cut(s) 789
Psp124BI GAGCTC 1 cut(s) 273
Psp5II RGGWCCY 1 cut(s) 1081
Psp6I CCWGG 1 cut(s) 1640
PspEI GGTNACC 2 cut(s) 1049, 1661
PspFI CCCAGC 2 cut(s) 1092, 1366
PspGI CCWGG 1 cut(s) 1640
PspN4I GGNNCC 6 cut(s) 151, 190, 328, 343, 422, 1083
PspPI GGNCC 7 cut(s) 220, 326, 342, 713, 948, 1081, 1267
PspPPI RGGWCCY 1 cut(s) 1081
PspXI VCTCGAGB 1 cut(s) 353
PsrI GAACNNNNNNTAC 2 cut(s) 1344, 1376
PstI CTGCAG 1 cut(s) 1496
PsyI GACNNNGTC 2 cut(s) 254, 1667
PteI GCGCGC 1 cut(s) 386
PvuII CAGCTG 1 cut(s) 1370
RsaI GTAC 2 cut(s) 736, 1539
RsaNI GTAC 2 cut(s) 735, 1538
SacI GAGCTC 1 cut(s) 273
SalI GTCGAC 1 cut(s) 248
SaqAI TTAA 3 cut(s) 38, 927, 1550
Sau96I GGNCC 7 cut(s) 220, 326, 342, 713, 948, 1081, 1267
SchI GAGTC 3 cut(s) 799, 983, 1265
ScrFI CCNGG 4 cut(s) 744, 760, 761, 1642
SduI GDGCHC 2 cut(s) 273, 1063
SfaNI GCATC 5 cut(s) 1031, 1225, 1233, 1429, 1484
SfcI CTRYAG 2 cut(s) 1492, 1556
Sfr274I CTCGAG 1 cut(s) 353
SgrAI CRCCGGYG 1 cut(s) 368
SinI GGWCC 4 cut(s) 220, 342, 948, 1081
SlaI CTCGAG 1 cut(s) 353
SmaI CCCGGG 1 cut(s) 761
SmlI CTYRAG 1 cut(s) 353
SmoI CTYRAG 1 cut(s) 353
Sse9I AATT 2 cut(s) 808, 1151
SseBI AGGCCT 1 cut(s) 1498
SspMI CTAG 3 cut(s) 794, 1055, 1188
SstI GAGCTC 1 cut(s) 273
StuI AGGCCT 1 cut(s) 1498
StyD4I CCNGG 4 cut(s) 742, 758, 759, 1640
StyI CCWWGG 2 cut(s) 834, 1054
TaaI ACNGT 4 cut(s) 677, 750, 948, 1560
TaiI ACGT 4 cut(s) 384, 432, 1348, 1549
TaqI TCGA 6 cut(s) 243, 249, 354, 1158, 1170, 1320
TasI AATT 2 cut(s) 808, 1151
TatI WGTACW 1 cut(s) 734
TauI GCSGC 6 cut(s) 108, 146, 303, 368, 375, 531
TfiI GAWTC 4 cut(s) 285, 508, 1160, 1645
Tru1I TTAA 3 cut(s) 38, 927, 1550
Tru9I TTAA 3 cut(s) 38, 927, 1550
TscAI CASTG 3 cut(s) 1224, 1565, 1635
TseFI GTSAC 4 cut(s) 256, 1049, 1582, 1661
TseI GCWGC 7 cut(s) 291, 754, 941, 1120, 1225, 1421, 1491
Tsp45I GTSAC 4 cut(s) 256, 1049, 1582, 1661
TspDTI ATGAA 6 cut(s) 392, 642, 675, 871, 1365, 1438
TspGWI ACGGA 7 cut(s) 61, 118, 157, 190, 529, 1555, 1707
TspMI CCCGGG 1 cut(s) 759
TspRI CASTG 3 cut(s) 1224, 1565, 1635
Tth111I GACNNNGTC 2 cut(s) 254, 1667
Van91I CCANNNNNTGG 2 cut(s) 840, 1373
VpaK11BI GGWCC 4 cut(s) 220, 342, 948, 1081
XapI RAATTY 1 cut(s) 808
XceI RCATGY 1 cut(s) 400
XcmI CCANNNNNNNNNTGG 2 cut(s) 841, 1432
XhoI CTCGAG 1 cut(s) 353
XmaI CCCGGG 1 cut(s) 759
XmaJI CCTAGG 1 cut(s) 1054
XmiI GTMKAC 1 cut(s) 249
XmnI GAANNNNTTC 1 cut(s) 284
XspI CTAG 3 cut(s) 794, 1055, 1188
ZraI GACGTC 1 cut(s) 430
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.