RchiOBHm_Chr4g0388881

Belongs to the pyruvate kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0009431
Physical Location & Seq
Forward (+)
4049404 .. 4055634
6231 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ36205

Sequence Viewer

Length: 1737 bp
ATGTCTCAGTCCCTTCACCTCCTCACCCCCACCACCTTAACCTTCCCCAAACCCCATTCCTCCTCCTCCTCCTCATTCCTCAACCCCTTCTCCACCCCCTATCGCCGGTTTCCGGTCACCACCGGCTTCCCCACCTTCTCCATCAAAGCCTCCGCCGCGGATCAAAAACCCTCCGTGCTCGTCTCCGGCAACGGCACCTCCTCCTCCGCGGTACCATCCCAGCAGGACTATGCTTCCTCCACCTCCATCGAGGTCGACGCCGTCACCGAGGCCGAGCTCCGGGAGAACGGCTTCCGAAGCACGCGGCGGACGAAGCTCGTCTGCACCATCGGCCCCGCCACCGCCGGCTTCGACCAGCTCGAGGCCCTCGCCGTCGGCGGCATGAACGTCGCCAGGCTCAACATGTGCCACGGCACGCGCGACTGGCACCGCGCGGTGATTGAGCGCGTGAGGAAGCTCAACGAGGACAAGGGCTACGCCGTCGCGATTATGATGGATACCGAAGGGAGTGAGATTCACATGGGCGATCTCGGCACTTCCTCCGTCAAAGCCGAGGATGGTGAGATATGGACTTTCAGTGTCAGAGCTTTCGGTTCTACTCTTCCGGAACGCACCATCAATGTGAATTACGAGGGTTTCGCCGAAGATGTGAAAGTGGGCGATGAGCTACTTGTGGATGGTGGAATGGTGAGGTTTGAGGTGGTTGAGAAGCTTGGCCCGGATGTCAAGTGTAGATGTACTGATCCCGGACTGTTGCTGCCGCGGGCTAACTTGACTTTCTGGAGGGATGGGAGTTTAGTGCGAGAACGAAATGCCATGCTTCCCACAATTTCTTCCAAGGATTGGTTGGACATTGATTTCGGGATTGCTGAAGGTGTTGATTTCATTGCTGTATCTTTTGTCAAGTCTGCTGAAGTGATCAATCACCTTAAAAGCTATATTGCAGCACGGTCTCGCGATATTTCTGTTGTGGCAAAGATAGAGAGTATTGACTCTCTAAAGAACTTGGAAGAGATCATCCTTGCATCAGATGGAGCAATGGTAGCAAGAGGTGACCTAGGTGCTCAGATACCATTGGAACAGGTCCCATCAGCCCAGCAAAAGATTGTTCAAGTATGTCGGCAGCTAAATAAGCCAGTCATTGTTGCTTCTCAACTACTCGAATCTATGATCGAGTATCCTACACCCACTAGAGCTGAAGTGGCCGATGTTTCTGAAGCCGTGAGGCAGCGAGCTGATGCTCTGATGCTTTCTGGTGAGTCAGCTATGGGCCAGTTCCCAGATAAGTCATTAGCTGTTCTCAGAAGTGTCAGTTTGAGGATCGAGAGGTGGTGGAGGGAAGAGAAACGTCATGAACCTATGTTACTCCCAGCTATTGGATCATCATTTTCAGACAGTATATCGGAGGAGCTTTGTATTTCTGCTGCCAAGATGGCAAATAATTTAGAGGTGGATGCTCTTTTCATCTTTACAAAGACGGGCCACATGGCATCTCTCCTGTCGCGCTGCCGGCCTGACTGCCCAATCTTTGCTTTTACCAACACAACATCTGTGCGGAGACGTCTGAACCTACAGTGGGGGCTGATACCATTTCGTGTTAGTTTCTCTGATGATATGGAAAGCAACCTTCACAAGACCTTCTCACTGCTGAAGGCCAGGAATCTGATCAAATCAGGTGACCTTGTCATTGCTGTCTCAGACATATTGCAGTCCATTCAAGTTATGAATGTACCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000287 GO:0003006 GO:0003674 GO:0003824 GO:0004743 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006629 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009791 GO:0009987 GO:0010035 GO:0010038 GO:0010154 GO:0010431 GO:0016052 GO:0016053 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0021700 GO:0022414 GO:0030955 GO:0031420 GO:0032501 GO:0032502 GO:0032504 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042221 GO:0042866 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046686 GO:0046700 GO:0046872 GO:0046939 GO:0048316 GO:0048608 GO:0048609 GO:0048731 GO:0048856 GO:0050896 GO:0051186 GO:0051188 GO:0055086 GO:0061458 GO:0071695 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00230 ko00620 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko04922 ko04930 ko05165 ko05203 ko05230 map00010 map00230 map00620 map01100 map01110 map01120 map01130 map01200 map01230 map04922 map04930 map05165 map05203 map05230
Pfam Domains
Protein Families
PK PK_C

Protein Analysis

578

Amino Acids

63.48

Weight (kDa)

5.75

Isoelectric Point (pI)

46.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK PF00224 102 - 430 7.4e-91 Pyruvate kinase, barrel domain
PK_C PF02887 469 - 567 2.3e-23 Pyruvate kinase, alpha/beta domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1564
Acc65I GGTACC 1 cut(s) 211
AccB1I GGYRCC 3 cut(s) 194, 211, 426
AccB7I CCANNNNNTGG 2 cut(s) 843, 1376
AccI GTMKAC 1 cut(s) 255
AccIII TCCGGA 1 cut(s) 604
AclWI GGATC 4 cut(s) 168, 737, 1328, 1387
AcoI YGGCCR 1 cut(s) 1203
AcuI CTGAAG 5 cut(s) 891, 933, 1218, 1236, 1670
AcyI GRCGYC 2 cut(s) 258, 1561
AfaI GTAC 3 cut(s) 213, 739, 1731
AfiI CCNNNNNNNGG 7 cut(s) 105, 112, 279, 361, 843, 1376, 1576
AflIII ACRYGT 1 cut(s) 402
AgsI TTSAA 2 cut(s) 1112, 1718
AjnI CCWGG 2 cut(s) 392, 1655
Alw21I GWGCWC 3 cut(s) 180, 279, 1066
Alw26I GTCTC 5 cut(s) 9, 187, 957, 1552, 1699
AlwI GGATC 4 cut(s) 168, 737, 1328, 1387
Ama87I CYCGRG 1 cut(s) 359
Aor13HI TCCGGA 1 cut(s) 604
AoxI GGCC 9 cut(s) 270, 331, 363, 715, 1203, 1270, 1480, 1511, 1653
ApeKI GCWGC 6 cut(s) 757, 944, 1123, 1228, 1424, 1506
ArsI GACNNNNNNTTYG 2 cut(s) 842, 874
Asp700I GAANNNNTTC 1 cut(s) 290
Asp718I GGTACC 1 cut(s) 211
AspA2I CCTAGG 1 cut(s) 1057
AspLEI GCGC 4 cut(s) 420, 434, 447, 1506
AspS9I GGNCC 6 cut(s) 332, 364, 716, 1084, 1270, 1480
AsuC2I CCSGG 3 cut(s) 281, 719, 747
AvaI CYCGRG 1 cut(s) 359
AvaII GGWCC 1 cut(s) 1084
AvrII CCTAGG 1 cut(s) 1057
BanI GGYRCC 3 cut(s) 194, 211, 426
BanII GRGCYC 1 cut(s) 279
Bbv12I GWGCWC 3 cut(s) 180, 279, 1066
BbvI GCAGC 6 cut(s) 744, 956, 1135, 1240, 1411, 1493
BceAI ACGGC 7 cut(s) 208, 245, 304, 356, 427, 464, 1205
BcgI CGANNNNNNTGC 2 cut(s) 370, 404
BciT130I CCWGG 2 cut(s) 394, 1657
BciVI GTATCC 2 cut(s) 490, 1188
BclI TGATCA 2 cut(s) 918, 1665
BcnI CCSGG 3 cut(s) 281, 719, 747
BcoDI GTCTC 5 cut(s) 9, 187, 957, 1552, 1699
BfaI CTAG 2 cut(s) 1058, 1191
BfmI CTRYAG 1 cut(s) 1571
BfuI GTATCC 2 cut(s) 490, 1188
BglI GCCNNNNNGGC 1 cut(s) 1433
BlnI CCTAGG 1 cut(s) 1057
Bme1390I CCNGG 5 cut(s) 281, 394, 719, 747, 1657
Bme18I GGWCC 1 cut(s) 1084
BmeT110I CYCGRG 1 cut(s) 359
BmgT120I GGNCC 6 cut(s) 332, 364, 716, 1084, 1270, 1480
BmiI GGNNCC 5 cut(s) 196, 213, 334, 428, 1086
BmrFI CCNGG 5 cut(s) 281, 394, 719, 747, 1657
BmsI GCATC 5 cut(s) 1034, 1228, 1236, 1444, 1499
BpmI CTGGAG 1 cut(s) 802
BpuMI CCSGG 3 cut(s) 281, 719, 747
BsaHI GRCGYC 2 cut(s) 258, 1561
BsaI GGTCTC 1 cut(s) 957
BsaJI CCNNGG 8 cut(s) 156, 207, 267, 409, 552, 761, 837, 1057
BsaWI WCCGGW 2 cut(s) 112, 604
BsaXI ACNNNNNCTCC 6 cut(s) 74, 104, 182, 212, 1026, 1056
Bsc4I CCNNNNNNNGG 7 cut(s) 105, 112, 279, 361, 843, 1376, 1576
Bse118I RCCGGY 4 cut(s) 105, 122, 344, 1509
Bse1I ACTGG 3 cut(s) 428, 1136, 1273
Bse3DI GCAATG 3 cut(s) 885, 1044, 1686
BseAI TCCGGA 1 cut(s) 604
BseBI CCWGG 2 cut(s) 394, 1657
BseDI CCNNGG 8 cut(s) 156, 207, 267, 409, 552, 761, 837, 1057
BseGI GGATG 7 cut(s) 215, 562, 682, 727, 793, 1017, 1459
BseLI CCNNNNNNNGG 7 cut(s) 105, 112, 279, 361, 843, 1376, 1576
BseMI GCAATG 3 cut(s) 885, 1044, 1686
BseMII CTCAG 4 cut(s) 20, 1079, 1315, 1710
BseNI ACTGG 3 cut(s) 428, 1136, 1273
BseRI GAGGAG 8 cut(s) 11, 52, 55, 58, 61, 190, 193, 1421
BseXI GCAGC 6 cut(s) 744, 956, 1135, 1240, 1411, 1493
BseYI CCCAGC 3 cut(s) 219, 1095, 1369
BsgI GTGCAG 1 cut(s) 307
BshFI GGCC 9 cut(s) 272, 333, 365, 717, 1205, 1272, 1482, 1513, 1655
BshNI GGYRCC 3 cut(s) 194, 211, 426
BsiHKAI GWGCWC 3 cut(s) 180, 279, 1066
BsiHKCI CYCGRG 1 cut(s) 359
BslFI GGGAC 1 cut(s) 1070
BslI CCNNNNNNNGG 7 cut(s) 105, 112, 279, 361, 843, 1376, 1576
BsmAI GTCTC 5 cut(s) 9, 187, 957, 1552, 1699
BsmBI CGTCTC 2 cut(s) 187, 1552
BsmFI GGGAC 1 cut(s) 1070
BsnI GGCC 9 cut(s) 272, 333, 365, 717, 1205, 1272, 1482, 1513, 1655
Bso31I GGTCTC 1 cut(s) 957
BsoBI CYCGRG 1 cut(s) 359
Bsp1286I GDGCHC 3 cut(s) 180, 279, 1066
Bsp13I TCCGGA 1 cut(s) 604
Bsp143I GATC 9 cut(s) 160, 526, 742, 918, 1014, 1170, 1320, 1379, 1665
Bsp68I TCGCGA 2 cut(s) 485, 957
BspANI GGCC 9 cut(s) 272, 333, 365, 717, 1205, 1272, 1482, 1513, 1655
BspCNI CTCAG 4 cut(s) 19, 1078, 1314, 1709
BspEI TCCGGA 1 cut(s) 604
BspHI TCATGA 1 cut(s) 1351
BspLI GGNNCC 5 cut(s) 196, 213, 334, 428, 1086
BspPI GGATC 4 cut(s) 168, 737, 1328, 1387
BspT107I GGYRCC 3 cut(s) 194, 211, 426
BspTNI GGTCTC 1 cut(s) 957
BsrDI GCAATG 3 cut(s) 885, 1044, 1686
BsrFI RCCGGY 4 cut(s) 105, 122, 344, 1509
BsrI ACTGG 3 cut(s) 428, 1136, 1273
BssAI RCCGGY 4 cut(s) 105, 122, 344, 1509
BssECI CCNNGG 8 cut(s) 156, 207, 267, 409, 552, 761, 837, 1057
BssMI GATC 9 cut(s) 160, 526, 742, 918, 1014, 1170, 1320, 1379, 1665
BssNI GRCGYC 2 cut(s) 258, 1561
BssT1I CCWWGG 2 cut(s) 837, 1057
Bst2UI CCWGG 2 cut(s) 394, 1657
Bst4CI ACNGT 5 cut(s) 753, 951, 1397, 1575, 1734
Bst6I CTCTTC 3 cut(s) 606, 1005, 1335
BstACI GRCGYC 2 cut(s) 258, 1561
BstC8I GCNNGC 6 cut(s) 302, 346, 416, 765, 1233, 1511
BstDEI CTNAG 4 cut(s) 6, 1065, 1301, 1696
BstDSI CCRYGG 4 cut(s) 156, 207, 409, 761
BstEII GGTNACC 3 cut(s) 115, 1052, 1676
BstF5I GGATG 7 cut(s) 215, 562, 682, 727, 793, 1017, 1459
BstHHI GCGC 4 cut(s) 420, 434, 447, 1506
BstKTI GATC 9 cut(s) 163, 529, 745, 921, 1017, 1173, 1323, 1382, 1668
BstMAI GTCTC 5 cut(s) 9, 187, 957, 1552, 1699
BstMBI GATC 9 cut(s) 160, 526, 742, 918, 1014, 1170, 1320, 1379, 1665
BstNI CCWGG 2 cut(s) 394, 1657
BstNSI RCATGY 1 cut(s) 406
BstPI GGTNACC 3 cut(s) 115, 1052, 1676
BstSCI CCNGG 5 cut(s) 279, 392, 717, 745, 1655
BstSFI CTRYAG 1 cut(s) 1571
BstV1I GCAGC 6 cut(s) 744, 956, 1135, 1240, 1411, 1493
BsuI GTATCC 2 cut(s) 490, 1188
BsuRI GGCC 9 cut(s) 272, 333, 365, 717, 1205, 1272, 1482, 1513, 1655
BtgI CCRYGG 4 cut(s) 156, 207, 409, 761
BtgZI GCGATG 1 cut(s) 675
BtsCI GGATG 7 cut(s) 215, 562, 682, 727, 793, 1017, 1459
BtsI GCAGTG 1 cut(s) 1643
BtsIMutI CAGTG 3 cut(s) 583, 1580, 1643
BtuMI TCGCGA 2 cut(s) 485, 957
Cac8I GCNNGC 6 cut(s) 302, 346, 416, 765, 1233, 1511
CciI TCATGA 1 cut(s) 1351
CfoI GCGC 4 cut(s) 420, 434, 447, 1506
Cfr10I RCCGGY 4 cut(s) 105, 122, 344, 1509
Cfr13I GGNCC 6 cut(s) 332, 364, 716, 1084, 1270, 1480
Cfr42I CCGCGG 3 cut(s) 159, 210, 764
CseI GACGC 1 cut(s) 266
Csp6I GTAC 3 cut(s) 212, 738, 1730
CviAII CATG 6 cut(s) 382, 403, 520, 817, 1352, 1486
CviQI GTAC 3 cut(s) 212, 738, 1730
DdeI CTNAG 4 cut(s) 6, 1065, 1301, 1696
DpnI GATC 9 cut(s) 162, 528, 744, 920, 1016, 1172, 1322, 1381, 1667
DpnII GATC 9 cut(s) 160, 526, 742, 918, 1014, 1170, 1320, 1379, 1665
EaeI YGGCCR 1 cut(s) 1203
Eam1104I CTCTTC 3 cut(s) 606, 1005, 1335
EarI CTCTTC 3 cut(s) 606, 1005, 1335
EciI GGCGGA 2 cut(s) 142, 322
Ecl136II GAGCTC 1 cut(s) 277
Eco130I CCWWGG 2 cut(s) 837, 1057
Eco24I GRGCYC 1 cut(s) 279
Eco31I GGTCTC 1 cut(s) 957
Eco47I GGWCC 1 cut(s) 1084
Eco53kI GAGCTC 1 cut(s) 277
Eco57I CTGAAG 5 cut(s) 891, 933, 1218, 1236, 1670
Eco88I CYCGRG 1 cut(s) 359
Eco91I GGTNACC 3 cut(s) 115, 1052, 1676
EcoICRI GAGCTC 1 cut(s) 277
EcoO109I RGGNCCY 2 cut(s) 364, 1084
EcoO65I GGTNACC 3 cut(s) 115, 1052, 1676
EcoRII CCWGG 2 cut(s) 392, 1655
EcoT14I CCWWGG 2 cut(s) 837, 1057
EcoT38I GRGCYC 1 cut(s) 279
ErhI CCWWGG 2 cut(s) 837, 1057
Esp3I CGTCTC 2 cut(s) 187, 1552
FaeI CATG 6 cut(s) 385, 406, 523, 820, 1355, 1489
FaqI GGGAC 1 cut(s) 1070
FatI CATG 6 cut(s) 381, 402, 519, 816, 1351, 1485
FauI CCCGC 2 cut(s) 343, 756
FbaI TGATCA 2 cut(s) 918, 1665
FblI GTMKAC 1 cut(s) 255
FokI GGATG 7 cut(s) 202, 569, 689, 734, 800, 1004, 1466
FriOI GRGCYC 1 cut(s) 279
FspBI CTAG 2 cut(s) 1058, 1191
GlaI GCGC 4 cut(s) 419, 433, 446, 1505
GsaI CCCAGC 3 cut(s) 223, 1099, 1373
GsuI CTGGAG 1 cut(s) 802
HaeIII GGCC 9 cut(s) 272, 333, 365, 717, 1205, 1272, 1482, 1513, 1655
HgaI GACGC 1 cut(s) 266
HhaI GCGC 4 cut(s) 420, 434, 447, 1506
Hin1I GRCGYC 2 cut(s) 258, 1561
Hin1II CATG 6 cut(s) 385, 406, 523, 820, 1355, 1489
Hin6I GCGC 4 cut(s) 418, 432, 445, 1504
HinP1I GCGC 4 cut(s) 418, 432, 445, 1504
HincII GTYRAC 1 cut(s) 256
HindII GTYRAC 1 cut(s) 256
HindIII AAGCTT 1 cut(s) 710
HinfI GANTC 5 cut(s) 514, 992, 1163, 1259, 1660
Hpy166II GTNNAC 1 cut(s) 256
Hpy188III TCNNGA 7 cut(s) 484, 605, 781, 862, 956, 1324, 1352
Hpy8I GTNNAC 1 cut(s) 256
Hpy99I CGWCG 4 cut(s) 260, 377, 392, 485
HpyAV CCTTC 9 cut(s) 23, 52, 97, 145, 497, 866, 1637, 1645, 1648
HpyCH4III ACNGT 5 cut(s) 753, 951, 1397, 1575, 1734
HpyCH4IV ACGT 3 cut(s) 387, 1348, 1561
HpyCH4V TGCA 4 cut(s) 324, 944, 1025, 1708
HpyF3I CTNAG 4 cut(s) 6, 1065, 1301, 1696
HpySE526I ACGT 3 cut(s) 387, 1348, 1561
Hsp92I GRCGYC 2 cut(s) 258, 1561
Hsp92II CATG 6 cut(s) 385, 406, 523, 820, 1355, 1489
HspAI GCGC 4 cut(s) 418, 432, 445, 1504
Kpn2I TCCGGA 1 cut(s) 604
KpnI GGTACC 1 cut(s) 215
KroI GCCGGC 2 cut(s) 344, 1509
KroNI GCCGGC 2 cut(s) 346, 1511
Ksp22I TGATCA 2 cut(s) 918, 1665
KspI CCGCGG 3 cut(s) 159, 210, 764
Kzo9I GATC 9 cut(s) 160, 526, 742, 918, 1014, 1170, 1320, 1379, 1665
LmnI GCTCC 3 cut(s) 282, 1034, 1408
Lsp1109I GCAGC 6 cut(s) 744, 956, 1135, 1240, 1411, 1493
LweI GCATC 5 cut(s) 1034, 1228, 1236, 1444, 1499
MaeI CTAG 2 cut(s) 1058, 1191
MaeII ACGT 3 cut(s) 387, 1348, 1561
MaeIII GTNAC 5 cut(s) 115, 262, 1052, 1362, 1676
MalI GATC 9 cut(s) 162, 528, 744, 920, 1016, 1172, 1322, 1381, 1667
MboI GATC 9 cut(s) 160, 526, 742, 918, 1014, 1170, 1320, 1379, 1665
MboII GAAGA 5 cut(s) 593, 656, 825, 1022, 1352
MhlI GDGCHC 3 cut(s) 180, 279, 1066
MluCI AATT 3 cut(s) 625, 828, 1441
MlyI GAGTC 2 cut(s) 986, 1268
MmeI TCCRAC 1 cut(s) 828
MroI TCCGGA 1 cut(s) 604
MroNI GCCGGC 2 cut(s) 344, 1509
MroXI GAANNNNTTC 1 cut(s) 290
MseI TTAA 2 cut(s) 38, 930
MslI CAYNNNNRTG 1 cut(s) 620
MspA1I CMGCKG 3 cut(s) 158, 209, 763
MspR9I CCNGG 5 cut(s) 281, 394, 719, 747, 1657
MvaI CCWGG 2 cut(s) 394, 1657
NaeI GCCGGC 2 cut(s) 346, 1511
NciI CCSGG 3 cut(s) 281, 719, 747
NdeII GATC 9 cut(s) 160, 526, 742, 918, 1014, 1170, 1320, 1379, 1665
NgoMIV GCCGGC 2 cut(s) 344, 1509
NlaIII CATG 6 cut(s) 385, 406, 523, 820, 1355, 1489
NlaIV GGNNCC 5 cut(s) 196, 213, 334, 428, 1086
NmeAIII GCCGAG 3 cut(s) 298, 510, 577
NmuCI GTSAC 4 cut(s) 115, 262, 1052, 1676
NruI TCGCGA 2 cut(s) 485, 957
NspI RCATGY 1 cut(s) 406
PaeR7I CTCGAG 1 cut(s) 359
PagI TCATGA 1 cut(s) 1351
PciI ACATGT 1 cut(s) 402
PcsI WCGNNNNNNNCGW 4 cut(s) 264, 308, 357, 417
PdiI GCCGGC 2 cut(s) 346, 1511
PdmI GAANNNNTTC 1 cut(s) 290
PfeI GAWTC 3 cut(s) 514, 1163, 1660
PflFI GACNNNGTC 2 cut(s) 260, 1682
PflMI CCANNNNNTGG 2 cut(s) 843, 1376
PfoI TCCNGGA 2 cut(s) 279, 745
PleI GAGTC 2 cut(s) 986, 1267
PpsI GAGTC 2 cut(s) 986, 1267
PpuMI RGGWCCY 1 cut(s) 1084
PscI ACATGT 1 cut(s) 402
Psp124BI GAGCTC 1 cut(s) 279
Psp5II RGGWCCY 1 cut(s) 1084
Psp6I CCWGG 2 cut(s) 392, 1655
PspEI GGTNACC 3 cut(s) 115, 1052, 1676
PspFI CCCAGC 3 cut(s) 219, 1095, 1369
PspGI CCWGG 2 cut(s) 392, 1655
PspN4I GGNNCC 5 cut(s) 196, 213, 334, 428, 1086
PspPI GGNCC 6 cut(s) 332, 364, 716, 1084, 1270, 1480
PspPPI RGGWCCY 1 cut(s) 1084
PspXI VCTCGAGB 1 cut(s) 359
PsrI GAACNNNNNNTAC 2 cut(s) 1347, 1379
PsyI GACNNNGTC 2 cut(s) 260, 1682
RruI TCGCGA 2 cut(s) 485, 957
RsaI GTAC 3 cut(s) 213, 739, 1731
RsaNI GTAC 3 cut(s) 212, 738, 1730
RseI CAYNNNNRTG 1 cut(s) 620
SacI GAGCTC 1 cut(s) 279
SacII CCGCGG 3 cut(s) 159, 210, 764
SalI GTCGAC 1 cut(s) 254
SaqAI TTAA 2 cut(s) 38, 930
Sau3AI GATC 9 cut(s) 160, 526, 742, 918, 1014, 1170, 1320, 1379, 1665
Sau96I GGNCC 6 cut(s) 332, 364, 716, 1084, 1270, 1480
SchI GAGTC 2 cut(s) 986, 1268
ScrFI CCNGG 5 cut(s) 281, 394, 719, 747, 1657
SduI GDGCHC 3 cut(s) 180, 279, 1066
SfaNI GCATC 5 cut(s) 1034, 1228, 1236, 1444, 1499
SfcI CTRYAG 1 cut(s) 1571
Sfr274I CTCGAG 1 cut(s) 359
Sfr303I CCGCGG 3 cut(s) 159, 210, 764
SgrBI CCGCGG 3 cut(s) 159, 210, 764
SinI GGWCC 1 cut(s) 1084
SlaI CTCGAG 1 cut(s) 359
SmiMI CAYNNNNRTG 1 cut(s) 620
SmlI CTYRAG 1 cut(s) 359
SmoI CTYRAG 1 cut(s) 359
Sse9I AATT 3 cut(s) 625, 828, 1441
SspMI CTAG 2 cut(s) 1058, 1191
SstI GAGCTC 1 cut(s) 279
StyD4I CCNGG 5 cut(s) 279, 392, 717, 745, 1655
StyI CCWWGG 2 cut(s) 837, 1057
TaaI ACNGT 5 cut(s) 753, 951, 1397, 1575, 1734
TaiI ACGT 3 cut(s) 390, 1351, 1564
TaqI TCGA 7 cut(s) 249, 255, 351, 360, 1161, 1173, 1323
TasI AATT 3 cut(s) 625, 828, 1441
TatI WGTACW 1 cut(s) 737
TauI GCSGC 4 cut(s) 158, 307, 381, 763
TfiI GAWTC 3 cut(s) 514, 1163, 1660
Tru1I TTAA 2 cut(s) 38, 930
Tru9I TTAA 2 cut(s) 38, 930
TscAI CASTG 3 cut(s) 583, 1580, 1650
TseFI GTSAC 4 cut(s) 115, 262, 1052, 1676
TseI GCWGC 6 cut(s) 757, 944, 1123, 1228, 1424, 1506
Tsp45I GTSAC 4 cut(s) 115, 262, 1052, 1676
TspDTI ATGAA 4 cut(s) 398, 874, 1368, 1453
TspGWI ACGGA 2 cut(s) 163, 532
TspRI CASTG 3 cut(s) 583, 1580, 1650
Tth111I GACNNNGTC 2 cut(s) 260, 1682
Van91I CCANNNNNTGG 2 cut(s) 843, 1376
VpaK11BI GGWCC 1 cut(s) 1084
XceI RCATGY 1 cut(s) 406
XcmI CCANNNNNNNNNTGG 1 cut(s) 844
XhoI CTCGAG 1 cut(s) 359
XmaJI CCTAGG 1 cut(s) 1057
XmiI GTMKAC 1 cut(s) 255
XmnI GAANNNNTTC 1 cut(s) 290
XspI CTAG 2 cut(s) 1058, 1191
ZraI GACGTC 1 cut(s) 1562
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.