MD16G1202700.v1.1

Belongs to the pyruvate kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0009431
Physical Location & Seq
Forward (+)
18452998 .. 18454980
1983 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1202700.v1.1.491

Sequence Viewer

Length: 960 bp
ATGGCCTGGTGGGGCCCACAAGCCCTTTGGCCTGGCCCTCGGTTGGAGACAGTTTTCGTGTCAAAACGGGCCATATTTTGGCCGATGGCCCTTTGGCCGGGTCGGTTGGAGATGGCCTTAGTTCTTTCAGTGTTAAGAGATTTGATATTTCTTTCCACCACCTATATATATTTTGTAAGCGATGTTGCTGTCATTGCAAAGATAGAGAGTATTGACTCACTAAAGAACTTGGAAGAGATCATTCAAGCATCAGATGGAGCAATGGTGGCAAGAGGAGATCTCGGTGCTCAGATACCACTAGAACAGGTCCCAGCAGCCCAGCAAAAGATTGTTCAAGTCTGTCGGCAGCTAAATAAGCCAGTCATTGTGGCTTCTCAACTACTTGAATCTATGATTGAATATCCTACACCCACTAGAGCCGAAGTGGCTGATGTTTCTGAAGCAGTGAGGCAGCGCGCTGATGCTTTGATGCTTTCTGGTGAGTCGGCTATGGGCCAGTTCCCAGAAAAGTCATTGGCTGTTCTTAGAAGTGTTAGTCTGAGGATTGAAAGATGGTGGAGGGAAGAGAAACGTCATGAAGCTATGGAACTGCCAGCTATTGGAACTTCATTTTCTGACAGTATTTCAGAGGAGATTTGCATTTCTGCTGCCAAGATGGCCAACAATTTGGAGGTAGATGCTCTTTTTGTCTATACAAAGACAGGCCACATGGCATCTCTCTTGTCTCGCTGCCGGCCAGACTGCCCAATCTTTGCTTTTACTAACACGACATCTGTACGGAGACGTCTGAACCTACAGTGGGGTCTGATACCGTTTAGGGTGAGCTTCTCCGATGATATGGAGAGCAACCTTAATAAAACCTTCTCGCTGCTGAAGGCCAGGAATTTGATCAAATCAGGCGACCTTGTTATTGCTGTCTCAGACGTCTTGCAGTGCATCCAAGTTATGAATGTTCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000287 GO:0003006 GO:0003674 GO:0003824 GO:0004743 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006629 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009791 GO:0009987 GO:0010035 GO:0010038 GO:0010154 GO:0010431 GO:0016052 GO:0016053 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0021700 GO:0022414 GO:0030955 GO:0031420 GO:0032501 GO:0032502 GO:0032504 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042221 GO:0042866 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046686 GO:0046700 GO:0046872 GO:0046939 GO:0048316 GO:0048608 GO:0048609 GO:0048731 GO:0048856 GO:0050896 GO:0051186 GO:0051188 GO:0055086 GO:0061458 GO:0071695 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00230 ko00620 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko04922 ko04930 ko05165 ko05203 ko05230 map00010 map00230 map00620 map01100 map01110 map01120 map01130 map01200 map01230 map04922 map04930 map05165 map05203 map05230
Pfam Domains
Protein Families
PK PK_C

Protein Analysis

320

Amino Acids

35.67

Weight (kDa)

5.88

Isoelectric Point (pI)

45.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK PF00224 61 - 171 4.8e-43 Pyruvate kinase, barrel domain
PK_C PF02887 210 - 308 6e-25 Pyruvate kinase, alpha/beta domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 787, 927
AccB7I CCANNNNNTGG 2 cut(s) 78, 599
AccII CGCG 1 cut(s) 456
AcoI YGGCCR 4 cut(s) 80, 95, 657, 734
AcsI RAATTY 1 cut(s) 883
AcuI CTGAAG 2 cut(s) 459, 893
AcyI GRCGYC 2 cut(s) 784, 924
AfaI GTAC 1 cut(s) 777
AfiI CCNNNNNNNGG 5 cut(s) 43, 78, 97, 599, 799
AgsI TTSAA 5 cut(s) 245, 335, 386, 398, 548
AjnI CCWGG 3 cut(s) 5, 31, 878
AluBI AGCT 4 cut(s) 349, 581, 596, 825
AluI AGCT 4 cut(s) 349, 581, 596, 825
Alw21I GWGCWC 1 cut(s) 289
Alw26I GTCTC 4 cut(s) 41, 729, 775, 922
ApaI GGGCCC 1 cut(s) 17
ApeKI GCWGC 6 cut(s) 314, 346, 451, 647, 729, 868
ApoI RAATTY 1 cut(s) 883
AspLEI GCGC 2 cut(s) 456, 458
AspS9I GGNCC 7 cut(s) 13, 14, 35, 69, 88, 307, 493
AsuC2I CCSGG 1 cut(s) 99
AsuHPI GGTGA 2 cut(s) 491, 832
AvaII GGWCC 1 cut(s) 307
BaeGI GKGCMC 1 cut(s) 17
BalI TGGCCA 1 cut(s) 659
BanII GRGCYC 1 cut(s) 17
Bbv12I GWGCWC 1 cut(s) 289
BbvI GCAGC 6 cut(s) 326, 358, 463, 634, 716, 855
BccI CCATC 5 cut(s) 79, 106, 248, 546, 649
BciT130I CCWGG 3 cut(s) 7, 33, 880
BclI TGATCA 1 cut(s) 888
BcnI CCSGG 1 cut(s) 99
BcoDI GTCTC 4 cut(s) 41, 729, 775, 922
BfaI CTAG 2 cut(s) 299, 414
BfmI CTRYAG 1 cut(s) 794
BglI GCCNNNNNGGC 2 cut(s) 425, 656
BglII AGATCT 1 cut(s) 277
BisI GCNGC 6 cut(s) 315, 347, 452, 648, 730, 869
BlsI GCNGC 6 cut(s) 316, 348, 453, 649, 731, 870
Bme1390I CCNGG 4 cut(s) 7, 33, 99, 880
Bme18I GGWCC 1 cut(s) 307
BmgT120I GGNCC 7 cut(s) 13, 14, 35, 69, 88, 307, 493
BmiI GGNNCC 3 cut(s) 14, 15, 309
BmrFI CCNGG 4 cut(s) 7, 33, 99, 880
BmsI GCATC 6 cut(s) 257, 451, 459, 667, 722, 945
BplI GAGNNNNNCTC 2 cut(s) 264, 296
BpuMI CCSGG 1 cut(s) 99
BsaHI GRCGYC 2 cut(s) 784, 924
BsaJI CCNNGG 1 cut(s) 38
BsaXI ACNNNNNCTCC 2 cut(s) 249, 279
Bsc4I CCNNNNNNNGG 5 cut(s) 43, 78, 97, 599, 799
Bse118I RCCGGY 1 cut(s) 732
Bse1I ACTGG 2 cut(s) 359, 496
Bse3DI GCAATG 2 cut(s) 192, 267
BseBI CCWGG 3 cut(s) 7, 33, 880
BseDI CCNNGG 1 cut(s) 38
BseGI GGATG 1 cut(s) 936
BseLI CCNNNNNNNGG 5 cut(s) 43, 78, 97, 599, 799
BseMI GCAATG 2 cut(s) 192, 267
BseMII CTCAG 3 cut(s) 302, 530, 933
BseNI ACTGG 2 cut(s) 359, 496
BsePI GCGCGC 1 cut(s) 454
BseRI GAGGAG 2 cut(s) 288, 644
BseSI GKGCMC 1 cut(s) 17
BseXI GCAGC 6 cut(s) 326, 358, 463, 634, 716, 855
BseYI CCCAGC 2 cut(s) 310, 318
Bsh1236I CGCG 1 cut(s) 456
BsiHKAI GWGCWC 1 cut(s) 289
BsiSI CCGG 2 cut(s) 98, 733
BslFI GGGAC 1 cut(s) 293
BslI CCNNNNNNNGG 5 cut(s) 43, 78, 97, 599, 799
BsmAI GTCTC 4 cut(s) 41, 729, 775, 922
BsmBI CGTCTC 1 cut(s) 775
BsmFI GGGAC 1 cut(s) 293
Bsp120I GGGCCC 1 cut(s) 13
Bsp1286I GDGCHC 2 cut(s) 17, 289
Bsp143I GATC 3 cut(s) 237, 277, 888
BspCNI CTCAG 3 cut(s) 301, 531, 932
BspFNI CGCG 1 cut(s) 456
BspHI TCATGA 1 cut(s) 574
BspLI GGNNCC 3 cut(s) 14, 15, 309
BsrDI GCAATG 2 cut(s) 192, 267
BsrFI RCCGGY 1 cut(s) 732
BsrI ACTGG 2 cut(s) 359, 496
BssAI RCCGGY 1 cut(s) 732
BssECI CCNNGG 1 cut(s) 38
BssHII GCGCGC 1 cut(s) 454
BssMI GATC 3 cut(s) 237, 277, 888
BssNI GRCGYC 2 cut(s) 784, 924
Bst2UI CCWGG 3 cut(s) 7, 33, 880
Bst4CI ACNGT 4 cut(s) 52, 620, 798, 813
Bst6I CTCTTC 2 cut(s) 228, 558
BstACI GRCGYC 2 cut(s) 784, 924
BstC8I GCNNGC 3 cut(s) 456, 594, 734
BstDEI CTNAG 5 cut(s) 118, 288, 524, 539, 919
BstF5I GGATG 1 cut(s) 936
BstFNI CGCG 1 cut(s) 456
BstHHI GCGC 2 cut(s) 456, 458
BstKTI GATC 3 cut(s) 240, 280, 891
BstMAI GTCTC 4 cut(s) 41, 729, 775, 922
BstMBI GATC 3 cut(s) 237, 277, 888
BstMWI GCNNNNNNNGC 5 cut(s) 194, 266, 355, 425, 656
BstNI CCWGG 3 cut(s) 7, 33, 880
BstSCI CCNGG 4 cut(s) 5, 31, 97, 878
BstSFI CTRYAG 1 cut(s) 794
BstSLI GKGCMC 1 cut(s) 17
BstUI CGCG 1 cut(s) 456
BstV1I GCAGC 6 cut(s) 326, 358, 463, 634, 716, 855
BstX2I RGATCY 1 cut(s) 277
BstXI CCANNNNNNTGG 1 cut(s) 667
BstYI RGATCY 1 cut(s) 277
BtgZI GCGATG 1 cut(s) 195
BtsCI GGATG 1 cut(s) 936
BtsI GCAGTG 2 cut(s) 450, 938
BtsIMutI CAGTG 4 cut(s) 135, 450, 803, 938
Cac8I GCNNGC 3 cut(s) 456, 594, 734
CciI TCATGA 1 cut(s) 574
CfoI GCGC 2 cut(s) 456, 458
Cfr10I RCCGGY 1 cut(s) 732
Cfr13I GGNCC 7 cut(s) 13, 14, 35, 69, 88, 307, 493
Csp6I GTAC 1 cut(s) 776
CviAII CATG 2 cut(s) 575, 709
CviQI GTAC 1 cut(s) 776
DdeI CTNAG 5 cut(s) 118, 288, 524, 539, 919
DpnI GATC 3 cut(s) 239, 279, 890
DpnII GATC 3 cut(s) 237, 277, 888
EaeI YGGCCR 4 cut(s) 80, 95, 657, 734
Eam1104I CTCTTC 2 cut(s) 228, 558
EarI CTCTTC 2 cut(s) 228, 558
Eco24I GRGCYC 1 cut(s) 17
Eco47I GGWCC 1 cut(s) 307
Eco57I CTGAAG 2 cut(s) 459, 893
EcoO109I RGGNCCY 2 cut(s) 13, 307
EcoRII CCWGG 3 cut(s) 5, 31, 878
EcoT38I GRGCYC 1 cut(s) 17
Esp3I CGTCTC 1 cut(s) 775
FaeI CATG 2 cut(s) 578, 712
FaqI GGGAC 1 cut(s) 293
FatI CATG 2 cut(s) 574, 708
FbaI TGATCA 1 cut(s) 888
Fnu4HI GCNGC 6 cut(s) 315, 347, 452, 648, 730, 869
FokI GGATG 1 cut(s) 923
FriOI GRGCYC 1 cut(s) 17
Fsp4HI GCNGC 6 cut(s) 315, 347, 452, 648, 730, 869
FspBI CTAG 2 cut(s) 299, 414
GlaI GCGC 2 cut(s) 455, 457
GluI GCNGC 6 cut(s) 315, 347, 452, 648, 730, 869
GsaI CCCAGC 2 cut(s) 314, 322
HapII CCGG 2 cut(s) 98, 733
HhaI GCGC 2 cut(s) 456, 458
Hin1I GRCGYC 2 cut(s) 784, 924
Hin1II CATG 2 cut(s) 578, 712
Hin6I GCGC 2 cut(s) 454, 456
HinP1I GCGC 2 cut(s) 454, 456
HinfI GANTC 3 cut(s) 215, 386, 482
HpaII CCGG 2 cut(s) 98, 733
HphI GGTGA 2 cut(s) 491, 832
Hpy188III TCNNGA 1 cut(s) 575
HpyAV CCTTC 2 cut(s) 868, 871
HpyCH4III ACNGT 4 cut(s) 52, 620, 798, 813
HpyCH4IV ACGT 3 cut(s) 571, 784, 924
HpyCH4V TGCA 4 cut(s) 197, 639, 931, 936
HpyF10VI GCNNNNNNNGC 5 cut(s) 194, 266, 355, 425, 656
HpyF3I CTNAG 5 cut(s) 118, 288, 524, 539, 919
HpySE526I ACGT 3 cut(s) 571, 784, 924
Hsp92I GRCGYC 2 cut(s) 784, 924
Hsp92II CATG 2 cut(s) 578, 712
HspAI GCGC 2 cut(s) 454, 456
KroI GCCGGC 1 cut(s) 732
KroNI GCCGGC 1 cut(s) 734
Ksp22I TGATCA 1 cut(s) 888
Kzo9I GATC 3 cut(s) 237, 277, 888
LmnI GCTCC 1 cut(s) 257
Lsp1109I GCAGC 6 cut(s) 326, 358, 463, 634, 716, 855
LweI GCATC 6 cut(s) 257, 451, 459, 667, 722, 945
MaeI CTAG 2 cut(s) 299, 414
MaeII ACGT 3 cut(s) 571, 784, 924
MalI GATC 3 cut(s) 239, 279, 890
MboI GATC 3 cut(s) 237, 277, 888
MboII GAAGA 2 cut(s) 245, 575
MflI RGATCY 1 cut(s) 277
MhlI GDGCHC 2 cut(s) 17, 289
MlsI TGGCCA 1 cut(s) 659
MluCI AATT 2 cut(s) 664, 883
MluNI TGGCCA 1 cut(s) 659
MlyI GAGTC 2 cut(s) 209, 491
MmeI TCCRAC 2 cut(s) 24, 87
MnlI CCTC 7 cut(s) 48, 266, 441, 534, 552, 622, 664
Mox20I TGGCCA 1 cut(s) 659
MroNI GCCGGC 1 cut(s) 732
MscI TGGCCA 1 cut(s) 659
MseI TTAA 2 cut(s) 134, 852
Msp20I TGGCCA 1 cut(s) 659
MspI CCGG 2 cut(s) 98, 733
MspR9I CCNGG 4 cut(s) 7, 33, 99, 880
MvaI CCWGG 3 cut(s) 7, 33, 880
MvnI CGCG 1 cut(s) 456
MwoI GCNNNNNNNGC 5 cut(s) 194, 266, 355, 425, 656
NaeI GCCGGC 1 cut(s) 734
NciI CCSGG 1 cut(s) 99
NdeII GATC 3 cut(s) 237, 277, 888
NgoMIV GCCGGC 1 cut(s) 732
NlaIII CATG 2 cut(s) 578, 712
NlaIV GGNNCC 3 cut(s) 14, 15, 309
PagI TCATGA 1 cut(s) 574
PauI GCGCGC 1 cut(s) 454
PdiI GCCGGC 1 cut(s) 734
PfeI GAWTC 1 cut(s) 386
PflMI CCANNNNNTGG 2 cut(s) 78, 599
PkrI GCNGC 6 cut(s) 316, 348, 453, 649, 731, 870
PleI GAGTC 2 cut(s) 209, 490
PpsI GAGTC 2 cut(s) 209, 490
PpuMI RGGWCCY 1 cut(s) 307
Psp5II RGGWCCY 1 cut(s) 307
Psp6I CCWGG 3 cut(s) 5, 31, 878
PspFI CCCAGC 2 cut(s) 310, 318
PspGI CCWGG 3 cut(s) 5, 31, 878
PspN4I GGNNCC 3 cut(s) 14, 15, 309
PspOMI GGGCCC 1 cut(s) 13
PspPI GGNCC 7 cut(s) 13, 14, 35, 69, 88, 307, 493
PspPPI RGGWCCY 1 cut(s) 307
PsuI RGATCY 1 cut(s) 277
PteI GCGCGC 1 cut(s) 454
RsaI GTAC 1 cut(s) 777
RsaNI GTAC 1 cut(s) 776
SaqAI TTAA 2 cut(s) 134, 852
SatI GCNGC 6 cut(s) 315, 347, 452, 648, 730, 869
Sau3AI GATC 3 cut(s) 237, 277, 888
Sau96I GGNCC 7 cut(s) 13, 14, 35, 69, 88, 307, 493
SchI GAGTC 2 cut(s) 209, 491
ScrFI CCNGG 4 cut(s) 7, 33, 99, 880
SduI GDGCHC 2 cut(s) 17, 289
SfaNI GCATC 6 cut(s) 257, 451, 459, 667, 722, 945
SfcI CTRYAG 1 cut(s) 794
SinI GGWCC 1 cut(s) 307
Sse9I AATT 2 cut(s) 664, 883
SspMI CTAG 2 cut(s) 299, 414
StyD4I CCNGG 4 cut(s) 5, 31, 97, 878
TaaI ACNGT 4 cut(s) 52, 620, 798, 813
TaiI ACGT 3 cut(s) 574, 787, 927
TasI AATT 2 cut(s) 664, 883
TfiI GAWTC 1 cut(s) 386
Tru1I TTAA 2 cut(s) 134, 852
Tru9I TTAA 2 cut(s) 134, 852
TscAI CASTG 4 cut(s) 135, 450, 803, 938
TseI GCWGC 6 cut(s) 314, 346, 451, 647, 729, 868
TspDTI ATGAA 2 cut(s) 591, 597
TspGWI ACGGA 1 cut(s) 793
TspRI CASTG 4 cut(s) 135, 450, 803, 938
Van91I CCANNNNNTGG 2 cut(s) 78, 599
VpaK11BI GGWCC 1 cut(s) 307
XapI RAATTY 1 cut(s) 883
XcmI CCANNNNNNNNNTGG 1 cut(s) 24
XspI CTAG 2 cut(s) 299, 414
ZraI GACGTC 2 cut(s) 785, 925
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.