Rmu_sc0000697.1_g000007

Belongs to the pyruvate kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000697.1
OG0009431
Physical Location & Seq
Reverse (-)
74763 .. 75824
1062 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000697.1_g000007.1.cds

Sequence Viewer

Length: 885 bp
atgtctcagtcccttcttcacctcctcacccccaccaccttaaccttccccaaacctcattcctcctcctcctcctcctcattcctcaaccccttctccaccccctatcgccggtttccggtcaccaccggcttccccaccttctccatcaaagcctccgccgcggatcaaaaaccctccgtgctcgtctccggcaacggcacctcctcctccgcggtacaatcgcagcaggactatgcttcctccacctccatcgaggtcgacgctgtcaccgaggccgagctccgggagaacggcttccgaagcacgcggcggacgaagctcgtctgcaccatcggccccgccaccgccggcttcgaccagctcgaggccctcgccgtcggcggcatgaacgtcgccaggctcaacatgtgccacggcacgcgcgactggcaccgcgcggtgattgagcgcgtgaggaagctcaacgaggacaagggctacgccgtcgcgattatgatggatacggaagggagtgagattcacatgggcgatctcggcacctcctccgtcaaagccgaggatggtgagatatggactttcagtgtcagagctttcggttctactcttccggaacgcaccatcaatgtgaattacgagggtttcgccgaagatgtgaaggtgggcgatgagctacttgtggatggtggaatggtgaggtttgaggtggttgagaagcttggcccggatgtcaagtgtaaatgtactgatcccggactgttgctgccgcgggctaacttgactttctggagggatgggagtttagtgcgagaacgaaatgccatgcttcccacaatttcttccaaggtttggcattggtttgcttttactcttgaatttcggtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000287 GO:0003006 GO:0003674 GO:0003824 GO:0004743 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006629 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009791 GO:0009987 GO:0010035 GO:0010038 GO:0010154 GO:0010431 GO:0016052 GO:0016053 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0021700 GO:0022414 GO:0030955 GO:0031420 GO:0032501 GO:0032502 GO:0032504 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042221 GO:0042866 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046686 GO:0046700 GO:0046872 GO:0046939 GO:0048316 GO:0048608 GO:0048609 GO:0048731 GO:0048856 GO:0050896 GO:0051186 GO:0051188 GO:0055086 GO:0061458 GO:0071695 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00230 ko00620 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko04922 ko04930 ko05165 ko05203 ko05230 map00010 map00230 map00620 map01100 map01110 map01120 map01130 map01200 map01230 map04922 map04930 map05165 map05203 map05230
Pfam Domains
Protein Families
PK PK_C

Protein Analysis

294

Amino Acids

32.31

Weight (kDa)

6.1

Isoelectric Point (pI)

44.41

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 200, 432, 539
AccB7I CCANNNNNTGG 1 cut(s) 849
AccI GTMKAC 1 cut(s) 261
AccIII TCCGGA 1 cut(s) 610
AclWI GGATC 2 cut(s) 174, 743
AcsI RAATTY 1 cut(s) 875
AfaI GTAC 2 cut(s) 219, 745
AfiI CCNNNNNNNGG 5 cut(s) 111, 118, 285, 367, 849
AflIII ACRYGT 1 cut(s) 408
AgsI TTSAA 1 cut(s) 875
AjnI CCWGG 1 cut(s) 398
AluBI AGCT 7 cut(s) 283, 322, 364, 463, 593, 673, 718
AluI AGCT 7 cut(s) 283, 322, 364, 463, 593, 673, 718
Alw21I GWGCWC 2 cut(s) 186, 285
Alw26I GTCTC 2 cut(s) 9, 193
AlwI GGATC 2 cut(s) 174, 743
Ama87I CYCGRG 1 cut(s) 365
Aor13HI TCCGGA 1 cut(s) 610
AoxI GGCC 4 cut(s) 276, 337, 369, 721
ApeKI GCWGC 2 cut(s) 226, 763
ApoI RAATTY 1 cut(s) 875
Asp700I GAANNNNTTC 1 cut(s) 296
AspLEI GCGC 3 cut(s) 426, 440, 453
AspS9I GGNCC 3 cut(s) 338, 370, 722
AsuC2I CCSGG 3 cut(s) 287, 725, 753
AsuHPI GGTGA 7 cut(s) 11, 19, 115, 262, 454, 578, 706
AvaI CYCGRG 1 cut(s) 365
BanI GGYRCC 3 cut(s) 200, 432, 539
BanII GRGCYC 1 cut(s) 285
Bbv12I GWGCWC 2 cut(s) 186, 285
BbvI GCAGC 2 cut(s) 238, 750
BccI CCATC 8 cut(s) 155, 260, 341, 493, 557, 629, 677, 788
BceAI ACGGC 5 cut(s) 214, 310, 362, 433, 470
BcgI CGANNNNNNTGC 2 cut(s) 376, 410
BciT130I CCWGG 1 cut(s) 400
BciVI GTATCC 1 cut(s) 496
BcnI CCSGG 3 cut(s) 287, 725, 753
BcoDI GTCTC 2 cut(s) 9, 193
BfuI GTATCC 1 cut(s) 496
BisI GCNGC 6 cut(s) 162, 227, 311, 385, 764, 767
BlsI GCNGC 6 cut(s) 163, 228, 312, 386, 765, 768
Bme1390I CCNGG 4 cut(s) 287, 400, 725, 753
BmeT110I CYCGRG 1 cut(s) 365
BmgT120I GGNCC 3 cut(s) 338, 370, 722
BmiI GGNNCC 4 cut(s) 202, 340, 434, 541
BmrFI CCNGG 4 cut(s) 287, 400, 725, 753
BpmI CTGGAG 1 cut(s) 808
BpuMI CCSGG 3 cut(s) 287, 725, 753
BsaJI CCNNGG 7 cut(s) 162, 213, 273, 415, 558, 767, 843
BsaWI WCCGGW 2 cut(s) 118, 610
BsaXI ACNNNNNCTCC 4 cut(s) 80, 110, 188, 218
Bsc4I CCNNNNNNNGG 5 cut(s) 111, 118, 285, 367, 849
Bse118I RCCGGY 3 cut(s) 111, 128, 350
Bse1I ACTGG 1 cut(s) 434
BseAI TCCGGA 1 cut(s) 610
BseBI CCWGG 1 cut(s) 400
BseDI CCNNGG 7 cut(s) 162, 213, 273, 415, 558, 767, 843
BseGI GGATG 4 cut(s) 568, 688, 733, 799
BseLI CCNNNNNNNGG 5 cut(s) 111, 118, 285, 367, 849
BseMII CTCAG 1 cut(s) 20
BseNI ACTGG 1 cut(s) 434
BseRI GAGGAG 9 cut(s) 14, 55, 58, 61, 64, 67, 196, 199, 535
BseXI GCAGC 2 cut(s) 238, 750
BsgI GTGCAG 1 cut(s) 313
BshFI GGCC 4 cut(s) 278, 339, 371, 723
BshNI GGYRCC 3 cut(s) 200, 432, 539
BsiHKAI GWGCWC 2 cut(s) 186, 285
BsiHKCI CYCGRG 1 cut(s) 365
BsiSI CCGG 9 cut(s) 112, 119, 129, 192, 286, 351, 611, 725, 753
BslI CCNNNNNNNGG 5 cut(s) 111, 118, 285, 367, 849
BsmAI GTCTC 2 cut(s) 9, 193
BsmBI CGTCTC 1 cut(s) 193
BsnI GGCC 4 cut(s) 278, 339, 371, 723
BsoBI CYCGRG 1 cut(s) 365
Bsp1286I GDGCHC 2 cut(s) 186, 285
Bsp13I TCCGGA 1 cut(s) 610
Bsp143I GATC 3 cut(s) 166, 532, 748
Bsp68I TCGCGA 1 cut(s) 491
BspANI GGCC 4 cut(s) 278, 339, 371, 723
BspCNI CTCAG 1 cut(s) 19
BspEI TCCGGA 1 cut(s) 610
BspLI GGNNCC 4 cut(s) 202, 340, 434, 541
BspPI GGATC 2 cut(s) 174, 743
BspT107I GGYRCC 3 cut(s) 200, 432, 539
BsrFI RCCGGY 3 cut(s) 111, 128, 350
BsrI ACTGG 1 cut(s) 434
BssAI RCCGGY 3 cut(s) 111, 128, 350
BssECI CCNNGG 7 cut(s) 162, 213, 273, 415, 558, 767, 843
BssMI GATC 3 cut(s) 166, 532, 748
BssT1I CCWWGG 1 cut(s) 843
Bst2UI CCWGG 1 cut(s) 400
Bst4CI ACNGT 1 cut(s) 759
Bst6I CTCTTC 1 cut(s) 612
BstC8I GCNNGC 4 cut(s) 308, 352, 422, 771
BstDEI CTNAG 1 cut(s) 6
BstDSI CCRYGG 4 cut(s) 162, 213, 415, 767
BstEII GGTNACC 1 cut(s) 121
BstF5I GGATG 4 cut(s) 568, 688, 733, 799
BstHHI GCGC 3 cut(s) 426, 440, 453
BstKTI GATC 3 cut(s) 169, 535, 751
BstMAI GTCTC 2 cut(s) 9, 193
BstMBI GATC 3 cut(s) 166, 532, 748
BstMWI GCNNNNNNNGC 6 cut(s) 161, 303, 319, 336, 430, 537
BstNI CCWGG 1 cut(s) 400
BstNSI RCATGY 1 cut(s) 412
BstPI GGTNACC 1 cut(s) 121
BstSCI CCNGG 4 cut(s) 285, 398, 723, 751
BstV1I GCAGC 2 cut(s) 238, 750
BsuI GTATCC 1 cut(s) 496
BsuRI GGCC 4 cut(s) 278, 339, 371, 723
BtgI CCRYGG 4 cut(s) 162, 213, 415, 767
BtgZI GCGATG 1 cut(s) 681
BtsCI GGATG 4 cut(s) 568, 688, 733, 799
BtsIMutI CAGTG 1 cut(s) 589
BtuMI TCGCGA 1 cut(s) 491
Cac8I GCNNGC 4 cut(s) 308, 352, 422, 771
CfoI GCGC 3 cut(s) 426, 440, 453
Cfr10I RCCGGY 3 cut(s) 111, 128, 350
Cfr13I GGNCC 3 cut(s) 338, 370, 722
Cfr42I CCGCGG 3 cut(s) 165, 216, 770
CseI GACGC 1 cut(s) 272
Csp6I GTAC 2 cut(s) 218, 744
CviAII CATG 4 cut(s) 388, 409, 526, 823
CviQI GTAC 2 cut(s) 218, 744
DdeI CTNAG 1 cut(s) 6
DpnI GATC 3 cut(s) 168, 534, 750
DpnII GATC 3 cut(s) 166, 532, 748
Eam1104I CTCTTC 1 cut(s) 612
EarI CTCTTC 1 cut(s) 612
EciI GGCGGA 2 cut(s) 148, 328
Ecl136II GAGCTC 1 cut(s) 283
Eco130I CCWWGG 1 cut(s) 843
Eco24I GRGCYC 1 cut(s) 285
Eco53kI GAGCTC 1 cut(s) 283
Eco88I CYCGRG 1 cut(s) 365
Eco91I GGTNACC 1 cut(s) 121
EcoICRI GAGCTC 1 cut(s) 283
EcoO109I RGGNCCY 1 cut(s) 370
EcoO65I GGTNACC 1 cut(s) 121
EcoRII CCWGG 1 cut(s) 398
EcoT14I CCWWGG 1 cut(s) 843
EcoT38I GRGCYC 1 cut(s) 285
ErhI CCWWGG 1 cut(s) 843
Esp3I CGTCTC 1 cut(s) 193
FaeI CATG 4 cut(s) 391, 412, 529, 826
FaiI YATR 7 cut(s) 237, 389, 410, 497, 527, 574, 824
FatI CATG 4 cut(s) 387, 408, 525, 822
FauI CCCGC 2 cut(s) 349, 762
FblI GTMKAC 1 cut(s) 261
Fnu4HI GCNGC 6 cut(s) 162, 227, 311, 385, 764, 767
FokI GGATG 4 cut(s) 575, 695, 740, 806
FriOI GRGCYC 1 cut(s) 285
Fsp4HI GCNGC 6 cut(s) 162, 227, 311, 385, 764, 767
GlaI GCGC 3 cut(s) 425, 439, 452
GluI GCNGC 6 cut(s) 162, 227, 311, 385, 764, 767
GsuI CTGGAG 1 cut(s) 808
HaeIII GGCC 4 cut(s) 278, 339, 371, 723
HapII CCGG 9 cut(s) 112, 119, 129, 192, 286, 351, 611, 725, 753
HgaI GACGC 1 cut(s) 272
HhaI GCGC 3 cut(s) 426, 440, 453
Hin1II CATG 4 cut(s) 391, 412, 529, 826
Hin6I GCGC 3 cut(s) 424, 438, 451
HinP1I GCGC 3 cut(s) 424, 438, 451
HincII GTYRAC 1 cut(s) 262
HindII GTYRAC 1 cut(s) 262
HindIII AAGCTT 1 cut(s) 716
HinfI GANTC 1 cut(s) 520
HpaII CCGG 9 cut(s) 112, 119, 129, 192, 286, 351, 611, 725, 753
HphI GGTGA 7 cut(s) 11, 19, 115, 262, 454, 578, 706
Hpy166II GTNNAC 1 cut(s) 262
Hpy188I TCNGA 2 cut(s) 302, 590
Hpy188III TCNNGA 4 cut(s) 490, 611, 787, 872
Hpy8I GTNNAC 1 cut(s) 262
Hpy99I CGWCG 4 cut(s) 266, 383, 398, 491
HpyAV CCTTC 6 cut(s) 23, 55, 103, 151, 503, 652
HpyCH4III ACNGT 1 cut(s) 759
HpyCH4IV ACGT 1 cut(s) 393
HpyCH4V TGCA 1 cut(s) 330
HpyF10VI GCNNNNNNNGC 6 cut(s) 161, 303, 319, 336, 430, 537
HpyF3I CTNAG 1 cut(s) 6
HpySE526I ACGT 1 cut(s) 393
Hsp92II CATG 4 cut(s) 391, 412, 529, 826
HspAI GCGC 3 cut(s) 424, 438, 451
Kpn2I TCCGGA 1 cut(s) 610
KroI GCCGGC 1 cut(s) 350
KroNI GCCGGC 1 cut(s) 352
KspI CCGCGG 3 cut(s) 165, 216, 770
Kzo9I GATC 3 cut(s) 166, 532, 748
LmnI GCTCC 1 cut(s) 288
Lsp1109I GCAGC 2 cut(s) 238, 750
MaeII ACGT 1 cut(s) 393
MaeIII GTNAC 2 cut(s) 121, 268
MalI GATC 3 cut(s) 168, 534, 750
MboI GATC 3 cut(s) 166, 532, 748
MboII GAAGA 4 cut(s) 8, 599, 662, 831
MhlI GDGCHC 2 cut(s) 186, 285
MluCI AATT 3 cut(s) 631, 834, 875
MroI TCCGGA 1 cut(s) 610
MroNI GCCGGC 1 cut(s) 350
MroXI GAANNNNTTC 1 cut(s) 296
MseI TTAA 1 cut(s) 41
MslI CAYNNNNRTG 1 cut(s) 626
MspA1I CMGCKG 3 cut(s) 164, 215, 769
MspI CCGG 9 cut(s) 112, 119, 129, 192, 286, 351, 611, 725, 753
MspR9I CCNGG 4 cut(s) 287, 400, 725, 753
MvaI CCWGG 1 cut(s) 400
MwoI GCNNNNNNNGC 6 cut(s) 161, 303, 319, 336, 430, 537
NaeI GCCGGC 1 cut(s) 352
NciI CCSGG 3 cut(s) 287, 725, 753
NdeII GATC 3 cut(s) 166, 532, 748
NgoMIV GCCGGC 1 cut(s) 350
NlaIII CATG 4 cut(s) 391, 412, 529, 826
NlaIV GGNNCC 4 cut(s) 202, 340, 434, 541
NmeAIII GCCGAG 3 cut(s) 304, 516, 583
NmuCI GTSAC 2 cut(s) 121, 268
NruI TCGCGA 1 cut(s) 491
NspI RCATGY 1 cut(s) 412
PaeR7I CTCGAG 1 cut(s) 365
PciI ACATGT 1 cut(s) 408
PcsI WCGNNNNNNNCGW 4 cut(s) 270, 314, 363, 423
PdiI GCCGGC 1 cut(s) 352
PdmI GAANNNNTTC 1 cut(s) 296
PfeI GAWTC 1 cut(s) 520
PflFI GACNNNGTC 1 cut(s) 266
PflMI CCANNNNNTGG 1 cut(s) 849
PfoI TCCNGGA 2 cut(s) 285, 751
PkrI GCNGC 6 cut(s) 163, 228, 312, 386, 765, 768
PscI ACATGT 1 cut(s) 408
Psp124BI GAGCTC 1 cut(s) 285
Psp6I CCWGG 1 cut(s) 398
PspEI GGTNACC 1 cut(s) 121
PspGI CCWGG 1 cut(s) 398
PspN4I GGNNCC 4 cut(s) 202, 340, 434, 541
PspPI GGNCC 3 cut(s) 338, 370, 722
PspXI VCTCGAGB 1 cut(s) 365
PsyI GACNNNGTC 1 cut(s) 266
RruI TCGCGA 1 cut(s) 491
RsaI GTAC 2 cut(s) 219, 745
RsaNI GTAC 2 cut(s) 218, 744
RseI CAYNNNNRTG 1 cut(s) 626
SacI GAGCTC 1 cut(s) 285
SacII CCGCGG 3 cut(s) 165, 216, 770
SalI GTCGAC 1 cut(s) 260
SaqAI TTAA 1 cut(s) 41
SatI GCNGC 6 cut(s) 162, 227, 311, 385, 764, 767
Sau3AI GATC 3 cut(s) 166, 532, 748
Sau96I GGNCC 3 cut(s) 338, 370, 722
ScrFI CCNGG 4 cut(s) 287, 400, 725, 753
SduI GDGCHC 2 cut(s) 186, 285
Sfr274I CTCGAG 1 cut(s) 365
Sfr303I CCGCGG 3 cut(s) 165, 216, 770
SgrBI CCGCGG 3 cut(s) 165, 216, 770
SlaI CTCGAG 1 cut(s) 365
SmiMI CAYNNNNRTG 1 cut(s) 626
SmlI CTYRAG 1 cut(s) 365
SmoI CTYRAG 1 cut(s) 365
Sse9I AATT 3 cut(s) 631, 834, 875
SstI GAGCTC 1 cut(s) 285
StyD4I CCNGG 4 cut(s) 285, 398, 723, 751
StyI CCWWGG 1 cut(s) 843
TaaI ACNGT 1 cut(s) 759
TaiI ACGT 1 cut(s) 396
TaqI TCGA 4 cut(s) 255, 261, 357, 366
TasI AATT 3 cut(s) 631, 834, 875
TatI WGTACW 1 cut(s) 743
TauI GCSGC 4 cut(s) 164, 313, 387, 769
TfiI GAWTC 1 cut(s) 520
Tru1I TTAA 1 cut(s) 41
Tru9I TTAA 1 cut(s) 41
TscAI CASTG 1 cut(s) 589
TseFI GTSAC 2 cut(s) 121, 268
TseI GCWGC 2 cut(s) 226, 763
Tsp45I GTSAC 2 cut(s) 121, 268
TspDTI ATGAA 1 cut(s) 404
TspGWI ACGGA 3 cut(s) 169, 521, 538
TspRI CASTG 1 cut(s) 589
Tth111I GACNNNGTC 1 cut(s) 266
Van91I CCANNNNNTGG 1 cut(s) 849
XapI RAATTY 1 cut(s) 875
XceI RCATGY 1 cut(s) 412
XhoI CTCGAG 1 cut(s) 365
XmiI GTMKAC 1 cut(s) 261
XmnI GAANNNNTTC 1 cut(s) 296
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.