pycom13g17570

Belongs to the pyruvate kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0009431
Physical Location & Seq
Forward (+)
13371882 .. 13375970
4089 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g17570.5

Sequence Viewer

Length: 1758 bp
ATGTCGCAGTCCATACATCTCCTCACCCCGTCCAACCTCGCCATCTCAAAACACCACTCCTCCTTCGTCTCCAACTTCAATCGCCGGTTGCCGGTCATCACCACCACCACCAAAACCACCGCCCCTTCTTTCCCAAAGCTCTCCATCAGAGCCTCCTCCTCCGATCCCGAGCCTTCCGTTCTCGTTACCGAAAACGGCACGTCCTCCTCGGGGCTCCAATCCCCTCCTTCGCAATCCGACCATGCTCTCTCCAGCTCCATCGAGGTCGACGCCGTCACCGAAACTGAGCTCCGGGAGAACGGATTCCGTTCCACCAGGCGCACCAAGCTCGTATGCACCATCGGCCCCGCCACCTTCGGATTCGACCAGCTCGAATCTCTCGCCGTTGGCGGCATGAACGTAGCGCGCATCAACATGTGCCACGGCACGCGCGAGTGGCACCGCGAGGTCATTCAGCGCGTCAGGAGGCTCAACGAGGACAAGGGATACGCCGTCGCCATCATGATGGATACCGAAGGTAGCGAGATTCATATGGGTGATTTGGGTGGCGCTTCCTCCGCCAAAGCTGAGGATGGTGAAGTATGGACTTTCAGTGTCAGAGCTTTCGGCTCCACTCTGCCCGAACACACCATCAATGTGAATTATGAAGGTTTTGCCGAAGATGTGAAAGTGGGGGATGAGCTGCTTGTGGATGGTGGGATGGTGAGATTCGAGGTGATCGAAAAGCTTGGTCCCGATGTTAAGTGTAAGTGTACCGATCCTGGTTTGTTGCTGCCTCGGGCTAATTTGACTTTCTGGAGGGACGGGAGTTTAGTGCGAGAACGTAATGCCATGCTTCCCACAATTTCGTCGAAGGATTGGTTGGACATTGATTTTGGGATTGCAGAGGGTGTTGATTTTATCGCTGTATCTTTTGTCAAATCTGCGGAAGTGATTAATCATCTTAAAAGCTATATTGCTGCACGGTCTCGAGATAGCGACATTGCTGTCATTGCAAAGATAGAGAGTATTGACTCTCTAAAGAACCTGGAAGAGATCATCCGAGCATCTGATGGAGCAATGGTAGCAAGAGGAGATCTAGGTGCTCAGATACCACTGGAACAGGTCCCAGCAGCCCAGCAAAAGATTGTTCAAGTTTGTCAGCAGCTAAATAAGCCAGTCATTGTGGCTTCTCAATTACTTGAATCTATGATTGAGTATCCTACACCGACTAGAGCTGAAGTGGCTGATGTTTCTGAAGCAGTGAGACAGCGCGCTGATGCTTTGATGCTTTCTGGTGAGTCGGCTATGGGCCAGTTCCCAGAAAAGTCATTGGCTGTTCTCAGAAGTGTTAGTCTGAGAATTGAAAGATGGTGGAGGGAAGAGAAACGTCATGAAGCTATGGAACTACCAACTATTGGATCTTCTTTTTCTGACAGTATCTCAGAGCAGATATGCATTTGTGCTGCCAAGATGGCCAATAATTTGGAGGTAGATGCTCTTTTTGTCTATACAAAGACAGGCCACATGGCATCTCTCTTGTCTCGCTGCCGGCCAGACTGCCCAATCTTTGCTTTTACTAACACCACATCTGTACGGAGACGTCTAAACCTACAGTGGGGTCTGATACCATTTAGGGTAAGCTTCTCCGATGATATGGAGAGCAACCTTAATAAAACCTTGTCACTGCTCAAGGCCAGGAACTTGATCGAATCAGGCGACCTTGTTATTGCTGTCTCAGACATCTTGCAGTGCATCCAAGTTATGAATGTTCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000287 GO:0003006 GO:0003674 GO:0003824 GO:0004743 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006629 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009791 GO:0009987 GO:0010035 GO:0010038 GO:0010154 GO:0010431 GO:0016052 GO:0016053 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0021700 GO:0022414 GO:0030955 GO:0031420 GO:0032501 GO:0032502 GO:0032504 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042221 GO:0042866 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046686 GO:0046700 GO:0046872 GO:0046939 GO:0048316 GO:0048608 GO:0048609 GO:0048731 GO:0048856 GO:0050896 GO:0051186 GO:0051188 GO:0055086 GO:0061458 GO:0071695 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00230 ko00620 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko04922 ko04930 ko05165 ko05203 ko05230 map00010 map00230 map00620 map01100 map01110 map01120 map01130 map01200 map01230 map04922 map04930 map05165 map05203 map05230
Pfam Domains
Protein Families
PK PK_C

Protein Analysis

586

Amino Acids

64.3

Weight (kDa)

5.48

Isoelectric Point (pI)

50.19

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 986
AatII GACGTC 1 cut(s) 1585
AccB1I GGYRCC 1 cut(s) 438
AccB7I CCANNNNNTGG 1 cut(s) 1397
AccI GTMKAC 1 cut(s) 267
AccII CGCG 6 cut(s) 406, 430, 432, 444, 459, 1254
AciI CCGC 6 cut(s) 120, 348, 390, 442, 558, 926
AclWI GGATC 3 cut(s) 158, 752, 1408
AcoI YGGCCR 2 cut(s) 1455, 1532
AcuI CTGAAG 2 cut(s) 1239, 1257
AcyI GRCGYC 2 cut(s) 270, 1582
AfaI GTAC 2 cut(s) 754, 1575
AfiI CCNNNNNNNGG 4 cut(s) 91, 210, 1397, 1597
AflIII ACRYGT 1 cut(s) 414
AgsI TTSAA 4 cut(s) 79, 1133, 1184, 1346
AjiI CACGTC 1 cut(s) 201
AjnI CCWGG 4 cut(s) 314, 760, 1026, 1676
AjuI GAANNNNNNNTTGG 2 cut(s) 845, 877
Alw21I GWGCWC 2 cut(s) 291, 1087
Alw26I GTCTC 6 cut(s) 73, 972, 1240, 1527, 1573, 1720
AlwI GGATC 3 cut(s) 158, 752, 1408
Ama87I CYCGRG 4 cut(s) 167, 208, 777, 969
AoxI GGCC 6 cut(s) 343, 1291, 1455, 1501, 1532, 1674
ApeKI GCWGC 7 cut(s) 682, 772, 959, 1112, 1144, 1445, 1527
ArsI GACNNNNNNTTYG 2 cut(s) 857, 889
AseI ATTAAT 1 cut(s) 936
Asp700I GAANNNNTTC 1 cut(s) 302
AspLEI GCGC 8 cut(s) 321, 406, 408, 432, 459, 551, 1254, 1256
AspS9I GGNCC 4 cut(s) 344, 731, 1105, 1291
AsuC2I CCSGG 1 cut(s) 293
AsuHPI GGTGA 8 cut(s) 16, 91, 268, 548, 587, 715, 727, 1289
AvaI CYCGRG 4 cut(s) 167, 208, 777, 969
AvaII GGWCC 2 cut(s) 731, 1105
BalI TGGCCA 1 cut(s) 1457
BanI GGYRCC 1 cut(s) 438
BanII GRGCYC 2 cut(s) 216, 291
Bbv12I GWGCWC 2 cut(s) 291, 1087
BbvCI CCTCAGC 1 cut(s) 567
BbvI GCAGC 7 cut(s) 669, 759, 946, 1124, 1156, 1432, 1514
BceAI ACGGC 5 cut(s) 211, 257, 368, 439, 476
BcgI CGANNNNNNTGC 2 cut(s) 310, 344
BciT130I CCWGG 4 cut(s) 316, 762, 1028, 1678
BciVI GTATCC 3 cut(s) 479, 502, 1209
BcnI CCSGG 1 cut(s) 293
BcoDI GTCTC 6 cut(s) 73, 972, 1240, 1527, 1573, 1720
BfaI CTAG 2 cut(s) 1079, 1212
BfmI CTRYAG 1 cut(s) 1592
BfoI RGCGCY 1 cut(s) 552
BfuI GTATCC 3 cut(s) 479, 502, 1209
BglI GCCNNNNNGGC 1 cut(s) 1454
BglII AGATCT 1 cut(s) 1075
BisI GCNGC 8 cut(s) 391, 683, 773, 960, 1113, 1145, 1446, 1528
BlsI GCNGC 8 cut(s) 392, 684, 774, 961, 1114, 1146, 1447, 1529
Bme1390I CCNGG 5 cut(s) 293, 316, 762, 1028, 1678
Bme18I GGWCC 2 cut(s) 731, 1105
BmeT110I CYCGRG 4 cut(s) 167, 208, 777, 969
BmgBI CACGTC 1 cut(s) 201
BmgT120I GGNCC 4 cut(s) 344, 731, 1105, 1291
BmiI GGNNCC 6 cut(s) 215, 346, 440, 610, 733, 1107
BmrFI CCNGG 5 cut(s) 293, 316, 762, 1028, 1678
BmsI GCATC 7 cut(s) 417, 1055, 1249, 1257, 1465, 1520, 1743
BpmI CTGGAG 2 cut(s) 235, 817
Bpu10I CCTNAGC 1 cut(s) 567
BpuEI CTTGAG 1 cut(s) 1655
BpuMI CCSGG 1 cut(s) 293
BsaHI GRCGYC 2 cut(s) 270, 1582
BsaI GGTCTC 1 cut(s) 972
BsaJI CCNNGG 3 cut(s) 207, 421, 776
BsaXI ACNNNNNCTCC 4 cut(s) 44, 74, 1047, 1077
Bsc4I CCNNNNNNNGG 4 cut(s) 91, 210, 1397, 1597
Bse118I RCCGGY 3 cut(s) 84, 91, 1530
Bse1I ACTGG 3 cut(s) 1101, 1157, 1294
Bse3DI GCAATG 3 cut(s) 981, 990, 1065
BseBI CCWGG 4 cut(s) 316, 762, 1028, 1678
BseDI CCNNGG 3 cut(s) 207, 421, 776
BseGI GGATG 6 cut(s) 577, 682, 697, 705, 1038, 1734
BseLI CCNNNNNNNGG 4 cut(s) 91, 210, 1397, 1597
BseMI GCAATG 3 cut(s) 981, 990, 1065
BseMII CTCAG 7 cut(s) 276, 558, 1100, 1328, 1336, 1437, 1731
BseNI ACTGG 3 cut(s) 1101, 1157, 1294
BsePI GCGCGC 2 cut(s) 404, 1252
BseRI GAGGAG 6 cut(s) 11, 49, 145, 148, 196, 1086
BseXI GCAGC 7 cut(s) 669, 759, 946, 1124, 1156, 1432, 1514
BseYI CCCAGC 2 cut(s) 1108, 1116
BsgI GTGCAG 1 cut(s) 945
Bsh1236I CGCG 6 cut(s) 406, 430, 432, 444, 459, 1254
BshFI GGCC 6 cut(s) 345, 1293, 1457, 1503, 1534, 1676
BshNI GGYRCC 1 cut(s) 438
BsiHKAI GWGCWC 2 cut(s) 291, 1087
BsiHKCI CYCGRG 4 cut(s) 167, 208, 777, 969
BsiSI CCGG 4 cut(s) 85, 92, 292, 1531
BslFI GGGAC 3 cut(s) 717, 815, 1091
BslI CCNNNNNNNGG 4 cut(s) 91, 210, 1397, 1597
BsmAI GTCTC 6 cut(s) 73, 972, 1240, 1527, 1573, 1720
BsmBI CGTCTC 2 cut(s) 73, 1573
BsmFI GGGAC 3 cut(s) 717, 815, 1091
BsnI GGCC 6 cut(s) 345, 1293, 1457, 1503, 1534, 1676
Bso31I GGTCTC 1 cut(s) 972
BsoBI CYCGRG 4 cut(s) 167, 208, 777, 969
Bsp1286I GDGCHC 3 cut(s) 216, 291, 1087
Bsp143I GATC 7 cut(s) 163, 717, 757, 1035, 1075, 1400, 1686
BspACI CCGC 6 cut(s) 120, 348, 390, 442, 558, 926
BspANI GGCC 6 cut(s) 345, 1293, 1457, 1503, 1534, 1676
BspCNI CTCAG 7 cut(s) 277, 559, 1099, 1329, 1335, 1436, 1730
BspFNI CGCG 6 cut(s) 406, 430, 432, 444, 459, 1254
BspHI TCATGA 2 cut(s) 501, 1372
BspLI GGNNCC 6 cut(s) 215, 346, 440, 610, 733, 1107
BspPI GGATC 3 cut(s) 158, 752, 1408
BspT107I GGYRCC 1 cut(s) 438
BspTNI GGTCTC 1 cut(s) 972
BsrDI GCAATG 3 cut(s) 981, 990, 1065
BsrFI RCCGGY 3 cut(s) 84, 91, 1530
BsrI ACTGG 3 cut(s) 1101, 1157, 1294
BssAI RCCGGY 3 cut(s) 84, 91, 1530
BssECI CCNNGG 3 cut(s) 207, 421, 776
BssHII GCGCGC 2 cut(s) 404, 1252
BssMI GATC 7 cut(s) 163, 717, 757, 1035, 1075, 1400, 1686
BssNI GRCGYC 2 cut(s) 270, 1582
Bst2UI CCWGG 4 cut(s) 316, 762, 1028, 1678
Bst4CI ACNGT 3 cut(s) 966, 1418, 1596
Bst6I CTCTTC 2 cut(s) 1026, 1356
BstACI GRCGYC 2 cut(s) 270, 1582
BstC8I GCNNGC 4 cut(s) 406, 428, 1254, 1532
BstDEI CTNAG 7 cut(s) 285, 567, 1086, 1322, 1337, 1423, 1717
BstDSI CCRYGG 1 cut(s) 421
BstF5I GGATG 6 cut(s) 577, 682, 697, 705, 1038, 1734
BstFNI CGCG 6 cut(s) 406, 430, 432, 444, 459, 1254
BstH2I RGCGCY 1 cut(s) 552
BstHHI GCGC 8 cut(s) 321, 406, 408, 432, 459, 551, 1254, 1256
BstKTI GATC 7 cut(s) 166, 720, 760, 1038, 1078, 1403, 1689
BstMAI GTCTC 6 cut(s) 73, 972, 1240, 1527, 1573, 1720
BstMBI GATC 7 cut(s) 163, 717, 757, 1035, 1075, 1400, 1686
BstMWI GCNNNNNNNGC 9 cut(s) 325, 342, 436, 557, 992, 1064, 1153, 1223, 1454
BstNI CCWGG 4 cut(s) 316, 762, 1028, 1678
BstNSI RCATGY 1 cut(s) 418
BstSCI CCNGG 5 cut(s) 291, 314, 760, 1026, 1676
BstSFI CTRYAG 1 cut(s) 1592
BstUI CGCG 6 cut(s) 406, 430, 432, 444, 459, 1254
BstV1I GCAGC 7 cut(s) 669, 759, 946, 1124, 1156, 1432, 1514
BstX2I RGATCY 2 cut(s) 1075, 1400
BstXI CCANNNNNNTGG 2 cut(s) 505, 1465
BstYI RGATCY 2 cut(s) 1075, 1400
BsuI GTATCC 3 cut(s) 479, 502, 1209
BsuRI GGCC 6 cut(s) 345, 1293, 1457, 1503, 1534, 1676
BtgI CCRYGG 1 cut(s) 421
BtrI CACGTC 1 cut(s) 201
BtsCI GGATG 6 cut(s) 577, 682, 697, 705, 1038, 1734
BtsI GCAGTG 3 cut(s) 1248, 1664, 1736
BtsIMutI CAGTG 6 cut(s) 598, 1094, 1248, 1601, 1664, 1736
Cac8I GCNNGC 4 cut(s) 406, 428, 1254, 1532
CciI TCATGA 2 cut(s) 501, 1372
CfoI GCGC 8 cut(s) 321, 406, 408, 432, 459, 551, 1254, 1256
Cfr10I RCCGGY 3 cut(s) 84, 91, 1530
Cfr13I GGNCC 4 cut(s) 344, 731, 1105, 1291
CseI GACGC 2 cut(s) 278, 448
Csp6I GTAC 2 cut(s) 753, 1574
CviAII CATG 7 cut(s) 242, 394, 415, 502, 832, 1373, 1507
CviQI GTAC 2 cut(s) 753, 1574
DdeI CTNAG 7 cut(s) 285, 567, 1086, 1322, 1337, 1423, 1717
DpnI GATC 7 cut(s) 165, 719, 759, 1037, 1077, 1402, 1688
DpnII GATC 7 cut(s) 163, 717, 757, 1035, 1075, 1400, 1686
DrdI GACNNNNNNGTC 1 cut(s) 986
DseDI GACNNNNNNGTC 1 cut(s) 986
EaeI YGGCCR 2 cut(s) 1455, 1532
Eam1104I CTCTTC 2 cut(s) 1026, 1356
EarI CTCTTC 2 cut(s) 1026, 1356
EciI GGCGGA 1 cut(s) 547
Ecl136II GAGCTC 1 cut(s) 289
Eco24I GRGCYC 2 cut(s) 216, 291
Eco31I GGTCTC 1 cut(s) 972
Eco47I GGWCC 2 cut(s) 731, 1105
Eco53kI GAGCTC 1 cut(s) 289
Eco57I CTGAAG 2 cut(s) 1239, 1257
Eco88I CYCGRG 4 cut(s) 167, 208, 777, 969
EcoICRI GAGCTC 1 cut(s) 289
EcoO109I RGGNCCY 1 cut(s) 1105
EcoRII CCWGG 4 cut(s) 314, 760, 1026, 1676
EcoT22I ATGCAT 1 cut(s) 1439
EcoT38I GRGCYC 2 cut(s) 216, 291
Esp3I CGTCTC 2 cut(s) 73, 1573
FaeI CATG 7 cut(s) 245, 397, 418, 505, 835, 1376, 1510
FaqI GGGAC 3 cut(s) 717, 815, 1091
FatI CATG 7 cut(s) 241, 393, 414, 501, 831, 1372, 1506
FauI CCCGC 1 cut(s) 355
FauNDI CATATG 1 cut(s) 531
FblI GTMKAC 1 cut(s) 267
Fnu4HI GCNGC 8 cut(s) 391, 683, 773, 960, 1113, 1145, 1446, 1528
FokI GGATG 6 cut(s) 584, 689, 704, 712, 1025, 1721
FriOI GRGCYC 2 cut(s) 216, 291
Fsp4HI GCNGC 8 cut(s) 391, 683, 773, 960, 1113, 1145, 1446, 1528
FspBI CTAG 2 cut(s) 1079, 1212
GlaI GCGC 8 cut(s) 320, 405, 407, 431, 458, 550, 1253, 1255
GluI GCNGC 8 cut(s) 391, 683, 773, 960, 1113, 1145, 1446, 1528
GsaI CCCAGC 2 cut(s) 1112, 1120
GsuI CTGGAG 2 cut(s) 235, 817
HaeII RGCGCY 1 cut(s) 552
HaeIII GGCC 6 cut(s) 345, 1293, 1457, 1503, 1534, 1676
HapII CCGG 4 cut(s) 85, 92, 292, 1531
HgaI GACGC 2 cut(s) 278, 448
HhaI GCGC 8 cut(s) 321, 406, 408, 432, 459, 551, 1254, 1256
Hin1I GRCGYC 2 cut(s) 270, 1582
Hin1II CATG 7 cut(s) 245, 397, 418, 505, 835, 1376, 1510
Hin6I GCGC 8 cut(s) 319, 404, 406, 430, 457, 549, 1252, 1254
HinP1I GCGC 8 cut(s) 319, 404, 406, 430, 457, 549, 1252, 1254
HincII GTYRAC 1 cut(s) 268
HindII GTYRAC 1 cut(s) 268
HindIII AAGCTT 2 cut(s) 725, 1621
HinfI GANTC 9 cut(s) 303, 360, 374, 526, 708, 1013, 1184, 1280, 1691
HpaII CCGG 4 cut(s) 85, 92, 292, 1531
HphI GGTGA 8 cut(s) 16, 91, 268, 548, 587, 715, 727, 1289
Hpy166II GTNNAC 2 cut(s) 268, 753
Hpy188III TCNNGA 8 cut(s) 167, 463, 502, 734, 796, 969, 971, 1373
Hpy8I GTNNAC 2 cut(s) 268, 753
Hpy99I CGWCG 3 cut(s) 272, 497, 853
HpyAV CCTTC 8 cut(s) 73, 135, 183, 237, 364, 509, 641, 847
HpyCH4III ACNGT 3 cut(s) 966, 1418, 1596
HpyCH4IV ACGT 5 cut(s) 200, 399, 823, 1369, 1582
HpyCH4V TGCA 7 cut(s) 336, 884, 962, 995, 1437, 1729, 1734
HpyF10VI GCNNNNNNNGC 9 cut(s) 325, 342, 436, 557, 992, 1064, 1153, 1223, 1454
HpyF3I CTNAG 7 cut(s) 285, 567, 1086, 1322, 1337, 1423, 1717
HpySE526I ACGT 5 cut(s) 200, 399, 823, 1369, 1582
Hsp92I GRCGYC 2 cut(s) 270, 1582
Hsp92II CATG 7 cut(s) 245, 397, 418, 505, 835, 1376, 1510
HspAI GCGC 8 cut(s) 319, 404, 406, 430, 457, 549, 1252, 1254
KroI GCCGGC 1 cut(s) 1530
KroNI GCCGGC 1 cut(s) 1532
Kzo9I GATC 7 cut(s) 163, 717, 757, 1035, 1075, 1400, 1686
LmnI GCTCC 5 cut(s) 219, 260, 294, 614, 1055
Lsp1109I GCAGC 7 cut(s) 669, 759, 946, 1124, 1156, 1432, 1514
LweI GCATC 7 cut(s) 417, 1055, 1249, 1257, 1465, 1520, 1743
MaeI CTAG 2 cut(s) 1079, 1212
MaeII ACGT 5 cut(s) 200, 399, 823, 1369, 1582
MaeIII GTNAC 3 cut(s) 184, 274, 1662
MalI GATC 7 cut(s) 165, 719, 759, 1037, 1077, 1402, 1688
MboI GATC 7 cut(s) 163, 717, 757, 1035, 1075, 1400, 1686
MboII GAAGA 4 cut(s) 671, 1043, 1373, 1395
MflI RGATCY 2 cut(s) 1075, 1400
MhlI GDGCHC 3 cut(s) 216, 291, 1087
MlsI TGGCCA 1 cut(s) 1457
MluCI AATT 6 cut(s) 640, 784, 843, 1175, 1341, 1462
MluNI TGGCCA 1 cut(s) 1457
MlyI GAGTC 2 cut(s) 1007, 1289
MmeI TCCRAC 4 cut(s) 57, 96, 261, 843
Mox20I TGGCCA 1 cut(s) 1457
Mph1103I ATGCAT 1 cut(s) 1439
MroNI GCCGGC 1 cut(s) 1530
MroXI GAANNNNTTC 1 cut(s) 302
MscI TGGCCA 1 cut(s) 1457
MseI TTAA 4 cut(s) 741, 936, 945, 1650
MslI CAYNNNNRTG 4 cut(s) 413, 503, 534, 635
Msp20I TGGCCA 1 cut(s) 1457
MspI CCGG 4 cut(s) 85, 92, 292, 1531
MspR9I CCNGG 5 cut(s) 293, 316, 762, 1028, 1678
MvaI CCWGG 4 cut(s) 316, 762, 1028, 1678
MvnI CGCG 6 cut(s) 406, 430, 432, 444, 459, 1254
MwoI GCNNNNNNNGC 9 cut(s) 325, 342, 436, 557, 992, 1064, 1153, 1223, 1454
NaeI GCCGGC 1 cut(s) 1532
NciI CCSGG 1 cut(s) 293
NdeI CATATG 1 cut(s) 531
NdeII GATC 7 cut(s) 163, 717, 757, 1035, 1075, 1400, 1686
NgoMIV GCCGGC 1 cut(s) 1530
NlaIII CATG 7 cut(s) 245, 397, 418, 505, 835, 1376, 1510
NlaIV GGNNCC 6 cut(s) 215, 346, 440, 610, 733, 1107
NmuCI GTSAC 2 cut(s) 274, 1662
NsiI ATGCAT 1 cut(s) 1439
NspI RCATGY 1 cut(s) 418
PaeR7I CTCGAG 1 cut(s) 969
PagI TCATGA 2 cut(s) 501, 1372
PauI GCGCGC 2 cut(s) 404, 1252
PciI ACATGT 1 cut(s) 414
PcsI WCGNNNNNNNCGW 5 cut(s) 276, 369, 429, 717, 1695
PdiI GCCGGC 1 cut(s) 1532
PdmI GAANNNNTTC 1 cut(s) 302
PfeI GAWTC 7 cut(s) 303, 360, 374, 526, 708, 1184, 1691
PflFI GACNNNGTC 1 cut(s) 272
PflMI CCANNNNNTGG 1 cut(s) 1397
PfoI TCCNGGA 1 cut(s) 291
PkrI GCNGC 8 cut(s) 392, 684, 774, 961, 1114, 1146, 1447, 1529
PleI GAGTC 2 cut(s) 1007, 1288
PpsI GAGTC 2 cut(s) 1007, 1288
PpuMI RGGWCCY 1 cut(s) 1105
PscI ACATGT 1 cut(s) 414
PshBI ATTAAT 1 cut(s) 936
Psp124BI GAGCTC 1 cut(s) 291
Psp5II RGGWCCY 1 cut(s) 1105
Psp6I CCWGG 4 cut(s) 314, 760, 1026, 1676
PspFI CCCAGC 2 cut(s) 1108, 1116
PspGI CCWGG 4 cut(s) 314, 760, 1026, 1676
PspN4I GGNNCC 6 cut(s) 215, 346, 440, 610, 733, 1107
PspPI GGNCC 4 cut(s) 344, 731, 1105, 1291
PspPPI RGGWCCY 1 cut(s) 1105
PsuI RGATCY 2 cut(s) 1075, 1400
PsyI GACNNNGTC 1 cut(s) 272
PteI GCGCGC 2 cut(s) 404, 1252
RsaI GTAC 2 cut(s) 754, 1575
RsaNI GTAC 2 cut(s) 753, 1574
RseI CAYNNNNRTG 4 cut(s) 413, 503, 534, 635
SacI GAGCTC 1 cut(s) 291
SalI GTCGAC 1 cut(s) 266
SaqAI TTAA 4 cut(s) 741, 936, 945, 1650
SatI GCNGC 8 cut(s) 391, 683, 773, 960, 1113, 1145, 1446, 1528
Sau3AI GATC 7 cut(s) 163, 717, 757, 1035, 1075, 1400, 1686
Sau96I GGNCC 4 cut(s) 344, 731, 1105, 1291
SchI GAGTC 2 cut(s) 1007, 1289
ScrFI CCNGG 5 cut(s) 293, 316, 762, 1028, 1678
SduI GDGCHC 3 cut(s) 216, 291, 1087
SfaNI GCATC 7 cut(s) 417, 1055, 1249, 1257, 1465, 1520, 1743
SfcI CTRYAG 1 cut(s) 1592
Sfr274I CTCGAG 1 cut(s) 969
SinI GGWCC 2 cut(s) 731, 1105
SlaI CTCGAG 1 cut(s) 969
SmiMI CAYNNNNRTG 4 cut(s) 413, 503, 534, 635
SmlI CTYRAG 2 cut(s) 969, 1670
SmoI CTYRAG 2 cut(s) 969, 1670
Sse9I AATT 6 cut(s) 640, 784, 843, 1175, 1341, 1462
SsiI CCGC 6 cut(s) 120, 348, 390, 442, 558, 926
SspMI CTAG 2 cut(s) 1079, 1212
SstI GAGCTC 1 cut(s) 291
StyD4I CCNGG 5 cut(s) 291, 314, 760, 1026, 1676
TaaI ACNGT 3 cut(s) 966, 1418, 1596
TaiI ACGT 5 cut(s) 203, 402, 826, 1372, 1585
TaqI TCGA 9 cut(s) 261, 267, 363, 372, 711, 720, 851, 970, 1689
TasI AATT 6 cut(s) 640, 784, 843, 1175, 1341, 1462
TauI GCSGC 1 cut(s) 393
TfiI GAWTC 7 cut(s) 303, 360, 374, 526, 708, 1184, 1691
Tru1I TTAA 4 cut(s) 741, 936, 945, 1650
Tru9I TTAA 4 cut(s) 741, 936, 945, 1650
TscAI CASTG 6 cut(s) 598, 1101, 1248, 1601, 1671, 1736
TseFI GTSAC 2 cut(s) 274, 1662
TseI GCWGC 7 cut(s) 682, 772, 959, 1112, 1144, 1445, 1527
Tsp45I GTSAC 2 cut(s) 274, 1662
TspDTI ATGAA 4 cut(s) 410, 518, 660, 1389
TspGWI ACGGA 4 cut(s) 166, 296, 315, 1591
TspRI CASTG 6 cut(s) 598, 1101, 1248, 1601, 1671, 1736
Tth111I GACNNNGTC 1 cut(s) 272
Van91I CCANNNNNTGG 1 cut(s) 1397
VpaK11BI GGWCC 2 cut(s) 731, 1105
VspI ATTAAT 1 cut(s) 936
XceI RCATGY 1 cut(s) 418
XhoI CTCGAG 1 cut(s) 969
XmiI GTMKAC 1 cut(s) 267
XmnI GAANNNNTTC 1 cut(s) 302
XspI CTAG 2 cut(s) 1079, 1212
ZraI GACGTC 1 cut(s) 1583
Zsp2I ATGCAT 1 cut(s) 1439
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.