MD16G1202600.v1.1

Belongs to the pyruvate kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0009431
Physical Location & Seq
Forward (+)
18440702 .. 18443162
2461 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1202600.v1.1.491

Sequence Viewer

Length: 1149 bp
ATGCCCATCAGTCTCCAGTCGTTTTTGCCCCTCCCCCATTTTCCTCGCTTTGGAAACTCCTCCCCAATTCCCAAAGCTATCCCCTCTTCGGTAACTCCGCCCAACTCTCCCCTTTTAAACTCTCCACCTACTTCCTCCGTGACCGTACACCGCCGGTCAACACCAGTCACAATGTCGCAGTCCGTACATCTCCTCACCCCTTCCGACCTCGCCATCCCAAAACACCACTCCTCCTTCCTCTCCACCTTCAATCGCCGGTTGTCGGTCACCACCACCACCACAACCGCTTCCCCTTTCTTCCCAAAGCTCTCCATCAGAGCCTCCTCCTCCGATCGCAAGCCTTCCGTTCTCGTCACTGACAACGGCACATCCTCCTCGGGGCTCCAATCCTCTGCTTCGCACGCCGACCATGCTCTCTCCAGCTCCATCGAGGTCGACGCCGTCACCGAAGCCGAGCTCCGGGAGAACGGATTCCGCTCCACCAGGCGCACCAAGCTCGTATGCACCATCGGTCCCGCCACCTCCGGATTCGACCAGCTCGAGTCGCTCGCCGTCGGCGGCATGAACGTAGCACGCATCAACATGTGCCACGGCACCCGCGAGTGGCACCGCGAGGTCATTCAGCGCGTCAGGAGGCTCAACGAGGACAAGGGATACGCCGTCGCCATCATGATGGACACCGAAGGCAGCGAGATTCATATGGGTGATTTGGGTGGCGCCTCCTCCGCCAAAGCCGAGGATGGTGAAGTATGGACTTTTAGTGTCAGAGCTTTCGGCTCCGCTCTCCCCGAGCACACCATCAATGTGAATTATGAAGGTTTTGCTGAAGATGTGAAAGTGGGGGATGAACTGCTTGTGGATGGCGGGATGGTGAGGTTTGAGGTGATCGAGAAGCTTGGTCCTGATGTTAAGTGTAAGTGTACTGATCCTGGTTTGTTGCTGCCTCGGGCTAATTTGACTTTCTGGAGGGACGGGAGTTTAGTGCGAGAACGCAATGCCATGCTTCCCACAATTTCGTCTAAGGATTGGTTGGACATTGATTTTGGGATTGCAGAGGGTGTTGATTTTATCGCTGTATCTTTTGTCAAGTCTGCGGAAGTGATTAATCATCTTAAAAGCTATATTGCTGCACGGTCACGAGATAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000287 GO:0003006 GO:0003674 GO:0003824 GO:0004743 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006629 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009791 GO:0009987 GO:0010035 GO:0010038 GO:0010154 GO:0010431 GO:0016052 GO:0016053 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0021700 GO:0022414 GO:0030955 GO:0031420 GO:0032501 GO:0032502 GO:0032504 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042221 GO:0042866 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046686 GO:0046700 GO:0046872 GO:0046939 GO:0048316 GO:0048608 GO:0048609 GO:0048731 GO:0048856 GO:0050896 GO:0051186 GO:0051188 GO:0055086 GO:0061458 GO:0071695 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00230 ko00620 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko04922 ko04930 ko05165 ko05203 ko05230 map00010 map00230 map00620 map01100 map01110 map01120 map01130 map01200 map01230 map04922 map04930 map05165 map05203 map05230
Pfam Domains
Protein Families
PK PK_C

Protein Analysis

383

Amino Acids

41.55

Weight (kDa)

6.64

Isoelectric Point (pI)

49.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK PF00224 162 - 375 6e-42 Pyruvate kinase, barrel domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 593, 606, 716
AccBSI CCGCTC 2 cut(s) 477, 782
AccI GTMKAC 1 cut(s) 435
AccII CGCG 3 cut(s) 600, 612, 627
AccIII TCCGGA 1 cut(s) 524
AclWI GGATC 1 cut(s) 920
AcuI CTGAAG 1 cut(s) 846
AcyI GRCGYC 2 cut(s) 438, 717
AfaI GTAC 3 cut(s) 147, 186, 922
AfiI CCNNNNNNNGG 6 cut(s) 50, 88, 262, 378, 459, 603
AflIII ACRYGT 1 cut(s) 582
AgsI TTSAA 1 cut(s) 250
AjnI CCWGG 2 cut(s) 482, 928
AluBI AGCT 9 cut(s) 77, 307, 423, 457, 496, 538, 770, 895, 1119
AluI AGCT 9 cut(s) 77, 307, 423, 457, 496, 538, 770, 895, 1119
Alw21I GWGCWC 2 cut(s) 459, 795
Alw26I GTCTC 1 cut(s) 17
AlwI GGATC 1 cut(s) 920
Ama87I CYCGRG 4 cut(s) 376, 539, 788, 945
Aor13HI TCCGGA 1 cut(s) 524
ApeKI GCWGC 3 cut(s) 687, 940, 1127
ArsI GACNNNNNNTTYG 2 cut(s) 1025, 1057
AseI ATTAAT 1 cut(s) 1104
Asp700I GAANNNNTTC 1 cut(s) 470
AspLEI GCGC 3 cut(s) 489, 627, 719
AspS9I GGNCC 2 cut(s) 512, 899
AsuC2I CCSGG 1 cut(s) 461
AsuHPI GGTGA 7 cut(s) 187, 259, 436, 716, 755, 883, 895
AvaI CYCGRG 4 cut(s) 376, 539, 788, 945
AvaII GGWCC 2 cut(s) 512, 899
BanI GGYRCC 3 cut(s) 593, 606, 716
BanII GRGCYC 2 cut(s) 384, 459
BauI CACGAG 1 cut(s) 1137
Bbv12I GWGCWC 2 cut(s) 459, 795
BbvI GCAGC 3 cut(s) 699, 927, 1114
BceAI ACGGC 5 cut(s) 379, 425, 536, 607, 644
BcgI CGANNNNNNTGC 2 cut(s) 478, 512
BciT130I CCWGG 2 cut(s) 484, 930
BciVI GTATCC 1 cut(s) 647
BcnI CCSGG 1 cut(s) 461
BcoDI GTCTC 1 cut(s) 17
BfoI RGCGCY 1 cut(s) 720
BfuI GTATCC 1 cut(s) 647
BisI GCNGC 4 cut(s) 559, 688, 941, 1128
BlsI GCNGC 4 cut(s) 560, 689, 942, 1129
Bme1390I CCNGG 3 cut(s) 461, 484, 930
Bme18I GGWCC 2 cut(s) 512, 899
BmeT110I CYCGRG 4 cut(s) 376, 539, 788, 945
BmgT120I GGNCC 2 cut(s) 512, 899
BmiI GGNNCC 6 cut(s) 383, 514, 595, 608, 718, 778
BmrFI CCNGG 3 cut(s) 461, 484, 930
BmsI GCATC 1 cut(s) 585
BpmI CTGGAG 2 cut(s) 403, 985
BpuMI CCSGG 1 cut(s) 461
BsaHI GRCGYC 2 cut(s) 438, 717
BsaJI CCNNGG 4 cut(s) 375, 589, 735, 944
BsaWI WCCGGW 1 cut(s) 524
BsaXI ACNNNNNCTCC 2 cut(s) 215, 245
Bsc4I CCNNNNNNNGG 6 cut(s) 50, 88, 262, 378, 459, 603
Bse118I RCCGGY 2 cut(s) 153, 255
Bse1I ACTGG 2 cut(s) 16, 164
Bse3DI GCAATG 1 cut(s) 1000
BseAI TCCGGA 1 cut(s) 524
BseBI CCWGG 2 cut(s) 484, 930
BseDI CCNNGG 4 cut(s) 375, 589, 735, 944
BseGI GGATG 6 cut(s) 213, 368, 745, 850, 865, 873
BseLI CCNNNNNNNGG 6 cut(s) 50, 88, 262, 378, 459, 603
BseMI GCAATG 1 cut(s) 1000
BseNI ACTGG 2 cut(s) 16, 164
BseRI GAGGAG 7 cut(s) 49, 182, 220, 313, 316, 364, 712
BseXI GCAGC 3 cut(s) 699, 927, 1114
BsgI GTGCAG 1 cut(s) 1113
Bsh1236I CGCG 3 cut(s) 600, 612, 627
Bsh1285I CGRYCG 1 cut(s) 334
BshNI GGYRCC 3 cut(s) 593, 606, 716
BsiEI CGRYCG 1 cut(s) 334
BsiHKAI GWGCWC 2 cut(s) 459, 795
BsiHKCI CYCGRG 4 cut(s) 376, 539, 788, 945
BsiSI CCGG 4 cut(s) 154, 256, 460, 525
BslFI GGGAC 2 cut(s) 498, 983
BslI CCNNNNNNNGG 6 cut(s) 50, 88, 262, 378, 459, 603
BsmAI GTCTC 1 cut(s) 17
BsmFI GGGAC 2 cut(s) 498, 983
BsoBI CYCGRG 4 cut(s) 376, 539, 788, 945
Bsp1286I GDGCHC 3 cut(s) 384, 459, 795
Bsp13I TCCGGA 1 cut(s) 524
Bsp143I GATC 3 cut(s) 331, 885, 925
BspEI TCCGGA 1 cut(s) 524
BspFNI CGCG 3 cut(s) 600, 612, 627
BspHI TCATGA 1 cut(s) 669
BspLI GGNNCC 6 cut(s) 383, 514, 595, 608, 718, 778
BspPI GGATC 1 cut(s) 920
BspT107I GGYRCC 3 cut(s) 593, 606, 716
BsrBI CCGCTC 2 cut(s) 477, 782
BsrDI GCAATG 1 cut(s) 1000
BsrFI RCCGGY 2 cut(s) 153, 255
BsrI ACTGG 2 cut(s) 16, 164
BssAI RCCGGY 2 cut(s) 153, 255
BssECI CCNNGG 4 cut(s) 375, 589, 735, 944
BssMI GATC 3 cut(s) 331, 885, 925
BssNI GRCGYC 2 cut(s) 438, 717
BssSI CACGAG 1 cut(s) 1137
Bst2BI CACGAG 1 cut(s) 1137
Bst2UI CCWGG 2 cut(s) 484, 930
Bst4CI ACNGT 2 cut(s) 145, 1134
Bst6I CTCTTC 1 cut(s) 91
BstACI GRCGYC 2 cut(s) 438, 717
BstC8I GCNNGC 4 cut(s) 338, 402, 549, 574
BstDEI CTNAG 1 cut(s) 1020
BstDSI CCRYGG 1 cut(s) 589
BstEII GGTNACC 1 cut(s) 265
BstF5I GGATG 6 cut(s) 213, 368, 745, 850, 865, 873
BstFNI CGCG 3 cut(s) 600, 612, 627
BstH2I RGCGCY 1 cut(s) 720
BstHHI GCGC 3 cut(s) 489, 627, 719
BstKTI GATC 3 cut(s) 334, 888, 928
BstMAI GTCTC 1 cut(s) 17
BstMBI GATC 3 cut(s) 331, 885, 925
BstMCI CGRYCG 1 cut(s) 334
BstMWI GCNNNNNNNGC 5 cut(s) 401, 410, 493, 544, 725
BstNI CCWGG 2 cut(s) 484, 930
BstNSI RCATGY 1 cut(s) 586
BstPI GGTNACC 1 cut(s) 265
BstSCI CCNGG 3 cut(s) 459, 482, 928
BstUI CGCG 3 cut(s) 600, 612, 627
BstV1I GCAGC 3 cut(s) 699, 927, 1114
BstXI CCANNNNNNTGG 1 cut(s) 673
BsuI GTATCC 1 cut(s) 647
BtgI CCRYGG 1 cut(s) 589
BtsCI GGATG 6 cut(s) 213, 368, 745, 850, 865, 873
BtsIMutI CAGTG 1 cut(s) 354
Cac8I GCNNGC 4 cut(s) 338, 402, 549, 574
CciI TCATGA 1 cut(s) 669
CfoI GCGC 3 cut(s) 489, 627, 719
Cfr10I RCCGGY 2 cut(s) 153, 255
Cfr13I GGNCC 2 cut(s) 512, 899
CseI GACGC 2 cut(s) 446, 616
Csp6I GTAC 3 cut(s) 146, 185, 921
CviAII CATG 5 cut(s) 410, 562, 583, 670, 1000
CviQI GTAC 3 cut(s) 146, 185, 921
DdeI CTNAG 1 cut(s) 1020
DinI GGCGCC 1 cut(s) 718
DpnI GATC 3 cut(s) 333, 887, 927
DpnII GATC 3 cut(s) 331, 885, 925
DraI TTTAAA 1 cut(s) 117
Eam1104I CTCTTC 1 cut(s) 91
EarI CTCTTC 1 cut(s) 91
EciI GGCGGA 2 cut(s) 87, 715
Ecl136II GAGCTC 1 cut(s) 457
Eco24I GRGCYC 2 cut(s) 384, 459
Eco47I GGWCC 2 cut(s) 512, 899
Eco53kI GAGCTC 1 cut(s) 457
Eco57I CTGAAG 1 cut(s) 846
Eco88I CYCGRG 4 cut(s) 376, 539, 788, 945
Eco91I GGTNACC 1 cut(s) 265
EcoICRI GAGCTC 1 cut(s) 457
EcoO65I GGTNACC 1 cut(s) 265
EcoRII CCWGG 2 cut(s) 482, 928
EcoT38I GRGCYC 2 cut(s) 384, 459
EgeI GGCGCC 1 cut(s) 718
EheI GGCGCC 1 cut(s) 718
FaeI CATG 5 cut(s) 413, 565, 586, 673, 1003
FaqI GGGAC 2 cut(s) 498, 983
FatI CATG 5 cut(s) 409, 561, 582, 669, 999
FauI CCCGC 3 cut(s) 523, 605, 857
FauNDI CATATG 1 cut(s) 699
FblI GTMKAC 1 cut(s) 435
Fnu4HI GCNGC 4 cut(s) 559, 688, 941, 1128
FokI GGATG 6 cut(s) 200, 355, 752, 857, 872, 880
FriOI GRGCYC 2 cut(s) 384, 459
Fsp4HI GCNGC 4 cut(s) 559, 688, 941, 1128
GlaI GCGC 3 cut(s) 488, 626, 718
GluI GCNGC 4 cut(s) 559, 688, 941, 1128
GsuI CTGGAG 2 cut(s) 403, 985
HaeII RGCGCY 1 cut(s) 720
HapII CCGG 4 cut(s) 154, 256, 460, 525
HgaI GACGC 2 cut(s) 446, 616
HhaI GCGC 3 cut(s) 489, 627, 719
Hin1I GRCGYC 2 cut(s) 438, 717
Hin1II CATG 5 cut(s) 413, 565, 586, 673, 1003
Hin6I GCGC 3 cut(s) 487, 625, 717
HinP1I GCGC 3 cut(s) 487, 625, 717
HincII GTYRAC 2 cut(s) 159, 436
HindII GTYRAC 2 cut(s) 159, 436
HindIII AAGCTT 1 cut(s) 893
HinfI GANTC 4 cut(s) 471, 528, 542, 694
HpaII CCGG 4 cut(s) 154, 256, 460, 525
HphI GGTGA 7 cut(s) 187, 259, 436, 716, 755, 883, 895
Hpy166II GTNNAC 4 cut(s) 148, 159, 436, 921
Hpy188I TCNGA 4 cut(s) 205, 317, 331, 767
Hpy188III TCNNGA 7 cut(s) 525, 631, 670, 889, 902, 964, 1137
Hpy8I GTNNAC 4 cut(s) 148, 159, 436, 921
Hpy99I CGWCG 3 cut(s) 440, 557, 665
HpyAV CCTTC 6 cut(s) 210, 244, 256, 351, 677, 809
HpyCH4III ACNGT 2 cut(s) 145, 1134
HpyCH4IV ACGT 1 cut(s) 567
HpyCH4V TGCA 3 cut(s) 504, 1052, 1130
HpyF10VI GCNNNNNNNGC 5 cut(s) 401, 410, 493, 544, 725
HpyF3I CTNAG 1 cut(s) 1020
HpySE526I ACGT 1 cut(s) 567
Hsp92I GRCGYC 2 cut(s) 438, 717
Hsp92II CATG 5 cut(s) 413, 565, 586, 673, 1003
HspAI GCGC 3 cut(s) 487, 625, 717
KasI GGCGCC 1 cut(s) 716
Kpn2I TCCGGA 1 cut(s) 524
Kzo9I GATC 3 cut(s) 331, 885, 925
LmnI GCTCC 5 cut(s) 387, 428, 462, 482, 782
Lsp1109I GCAGC 3 cut(s) 699, 927, 1114
LweI GCATC 1 cut(s) 585
MaeII ACGT 1 cut(s) 567
MaeIII GTNAC 7 cut(s) 91, 139, 166, 265, 352, 442, 1134
MalI GATC 3 cut(s) 333, 887, 927
MbiI CCGCTC 2 cut(s) 477, 782
MboI GATC 3 cut(s) 331, 885, 925
MboII GAAGA 3 cut(s) 78, 289, 839
MhlI GDGCHC 3 cut(s) 384, 459, 795
MluCI AATT 4 cut(s) 66, 808, 952, 1011
Mly113I GGCGCC 1 cut(s) 717
MlyI GAGTC 1 cut(s) 551
MmeI TCCRAC 2 cut(s) 228, 1011
MroI TCCGGA 1 cut(s) 524
MroXI GAANNNNTTC 1 cut(s) 470
MseI TTAA 4 cut(s) 116, 909, 1104, 1113
MslI CAYNNNNRTG 4 cut(s) 581, 671, 702, 803
MspI CCGG 4 cut(s) 154, 256, 460, 525
MspR9I CCNGG 3 cut(s) 461, 484, 930
MvaI CCWGG 2 cut(s) 484, 930
MvnI CGCG 3 cut(s) 600, 612, 627
MwoI GCNNNNNNNGC 5 cut(s) 401, 410, 493, 544, 725
NarI GGCGCC 1 cut(s) 717
NciI CCSGG 1 cut(s) 461
NdeI CATATG 1 cut(s) 699
NdeII GATC 3 cut(s) 331, 885, 925
NlaIII CATG 5 cut(s) 413, 565, 586, 673, 1003
NlaIV GGNNCC 6 cut(s) 383, 514, 595, 608, 718, 778
NmeAIII GCCGAG 2 cut(s) 478, 760
NmuCI GTSAC 6 cut(s) 139, 166, 265, 352, 442, 1134
NspI RCATGY 1 cut(s) 586
PaeR7I CTCGAG 1 cut(s) 539
PagI TCATGA 1 cut(s) 669
PciI ACATGT 1 cut(s) 582
PcsI WCGNNNNNNNCGW 3 cut(s) 444, 537, 597
PdmI GAANNNNTTC 1 cut(s) 470
PfeI GAWTC 3 cut(s) 471, 528, 694
PflFI GACNNNGTC 1 cut(s) 440
PfoI TCCNGGA 1 cut(s) 459
PkrI GCNGC 4 cut(s) 560, 689, 942, 1129
Ple19I CGATCG 1 cut(s) 334
PleI GAGTC 1 cut(s) 550
PluTI GGCGCC 1 cut(s) 720
PpsI GAGTC 1 cut(s) 550
PscI ACATGT 1 cut(s) 582
PshBI ATTAAT 1 cut(s) 1104
Psp124BI GAGCTC 1 cut(s) 459
Psp6I CCWGG 2 cut(s) 482, 928
PspEI GGTNACC 1 cut(s) 265
PspGI CCWGG 2 cut(s) 482, 928
PspN4I GGNNCC 6 cut(s) 383, 514, 595, 608, 718, 778
PspPI GGNCC 2 cut(s) 512, 899
PspXI VCTCGAGB 1 cut(s) 539
PsyI GACNNNGTC 1 cut(s) 440
PvuI CGATCG 1 cut(s) 334
RsaI GTAC 3 cut(s) 147, 186, 922
RsaNI GTAC 3 cut(s) 146, 185, 921
RseI CAYNNNNRTG 4 cut(s) 581, 671, 702, 803
SacI GAGCTC 1 cut(s) 459
SalI GTCGAC 1 cut(s) 434
SaqAI TTAA 4 cut(s) 116, 909, 1104, 1113
SatI GCNGC 4 cut(s) 559, 688, 941, 1128
Sau3AI GATC 3 cut(s) 331, 885, 925
Sau96I GGNCC 2 cut(s) 512, 899
SchI GAGTC 1 cut(s) 551
ScrFI CCNGG 3 cut(s) 461, 484, 930
SduI GDGCHC 3 cut(s) 384, 459, 795
SfaNI GCATC 1 cut(s) 585
SfoI GGCGCC 1 cut(s) 718
Sfr274I CTCGAG 1 cut(s) 539
SinI GGWCC 2 cut(s) 512, 899
SlaI CTCGAG 1 cut(s) 539
SmiMI CAYNNNNRTG 4 cut(s) 581, 671, 702, 803
SmlI CTYRAG 1 cut(s) 539
SmoI CTYRAG 1 cut(s) 539
Sse9I AATT 4 cut(s) 66, 808, 952, 1011
SspDI GGCGCC 1 cut(s) 716
SstI GAGCTC 1 cut(s) 459
StyD4I CCNGG 3 cut(s) 459, 482, 928
TaaI ACNGT 2 cut(s) 145, 1134
TaiI ACGT 1 cut(s) 570
TaqI TCGA 5 cut(s) 429, 435, 531, 540, 888
TaqII GACCGA 2 cut(s) 253, 500
TasI AATT 4 cut(s) 66, 808, 952, 1011
TatI WGTACW 1 cut(s) 920
TauI GCSGC 1 cut(s) 561
TfiI GAWTC 3 cut(s) 471, 528, 694
Tru1I TTAA 4 cut(s) 116, 909, 1104, 1113
Tru9I TTAA 4 cut(s) 116, 909, 1104, 1113
TscAI CASTG 1 cut(s) 361
TseFI GTSAC 6 cut(s) 139, 166, 265, 352, 442, 1134
TseI GCWGC 3 cut(s) 687, 940, 1127
Tsp45I GTSAC 6 cut(s) 139, 166, 265, 352, 442, 1134
TspDTI ATGAA 4 cut(s) 578, 686, 828, 861
TspGWI ACGGA 4 cut(s) 127, 172, 334, 483
TspRI CASTG 1 cut(s) 361
Tth111I GACNNNGTC 1 cut(s) 440
VpaK11BI GGWCC 2 cut(s) 512, 899
VspI ATTAAT 1 cut(s) 1104
XceI RCATGY 1 cut(s) 586
XhoI CTCGAG 1 cut(s) 539
XmiI GTMKAC 1 cut(s) 435
XmnI GAANNNNTTC 1 cut(s) 470
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.