MD13G1202600.v1.1

Belongs to the pyruvate kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0009431
Physical Location & Seq
Forward (+)
18152558 .. 18154835
2278 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1202600.v1.1.491

Sequence Viewer

Length: 969 bp
ATGCTAGCAGTGACATGCATTTGTGATTGTTATTCATTTCTTATGATCTTGCTAAAATCTACTTGTGATTGGTTGGACATTGATTTTGGGATTGCAGAGGGTGTTGATTTTATCGCTGTATCTTTTGTCAAATCTGCGGAAGTGATAAATCATCTTAAAAGCTATATTGCTGCACGGTCTCGAGATAGCGACATTGCTGTCATTGCAAAGATAGAGAGCATTGACTCTCTAAAGAACCTGGAAGAGATCATCCGAGCATCAGATGGAGCAATGGTAGCAAGAGGGGATCTAGGTGCTCAGATACCACTGGAACAGGTCCCAGCAGCCCAGCAAAAGATTGTTCAAGTTTGTCGGCAGCTAAATAAGCCAGTCATTGTGGCTTCTCAATTACTTGAATCTATGATTGAATATCCTACACCCACTAGAGCCGAAGTGGCTGATGTTTCTGAAGCAGTGAGACAGCGCGCTGATGCTTTGATGCTTTCTGGTGAGTCGGCTATGGGCCAGTTCCCTGAAAAGTCATTGGCTGTTCTCAGAAGTGTTAGTCTGAGAATTGAAAGATGGTGGAGGGAAGAGAAACGTCATGAAGCTATGGAACTACCAGCTATTGGATCTTCATTTTCAGACAGTATCTCAGAGCAGATTTGCATTTGTGCTGCCAAGATGGCCAATAATTTGGAGGTAGATGCTCTTTTTGTCTATACAAAGACAGGCCACATGGCATCTCTCTTGTCTCGCTGCCGGCCAGACTGCCCAATCTTTGCTTTTACTAACACGACATCTGTACGGAGACGCCTAAACCTACAGTGGGGGCTGATACCATTTAGGGTGAGCTTCTCGGATGACATGGAGAGCAACCTTAACAAAACCTTGTCACTGCTCAAGGCCAGGAATTTGATCAAATCAGGCGACCTTGTTATTGCTGTCTCAGACATCTTGCAGTGCATCCAAGTTATGAATGTTCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000287 GO:0003006 GO:0003674 GO:0003824 GO:0004743 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006629 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009791 GO:0009987 GO:0010035 GO:0010038 GO:0010154 GO:0010431 GO:0016052 GO:0016053 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0021700 GO:0022414 GO:0030955 GO:0031420 GO:0032501 GO:0032502 GO:0032504 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042221 GO:0042866 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046686 GO:0046700 GO:0046872 GO:0046939 GO:0048316 GO:0048608 GO:0048609 GO:0048731 GO:0048856 GO:0050896 GO:0051186 GO:0051188 GO:0055086 GO:0061458 GO:0071695 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00230 ko00620 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko04922 ko04930 ko05165 ko05203 ko05230 map00010 map00230 map00620 map01100 map01110 map01120 map01130 map01200 map01230 map04922 map04930 map05165 map05203 map05230
Pfam Domains
Protein Families
PK PK_C

Protein Analysis

323

Amino Acids

35.68

Weight (kDa)

5.43

Isoelectric Point (pI)

44.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK PF00224 23 - 174 5.7e-53 Pyruvate kinase, barrel domain
PK_C PF02887 213 - 311 1.6e-24 Pyruvate kinase, alpha/beta domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 197
AccB7I CCANNNNNTGG 1 cut(s) 608
AccII CGCG 1 cut(s) 465
AciI CCGC 1 cut(s) 137
AclWI GGATC 2 cut(s) 294, 619
AcoI YGGCCR 2 cut(s) 666, 743
AcsI RAATTY 1 cut(s) 892
AcuI CTGAAG 1 cut(s) 468
AcyI GRCGYC 1 cut(s) 793
AfaI GTAC 1 cut(s) 786
AfiI CCNNNNNNNGG 2 cut(s) 608, 808
AgsI TTSAA 4 cut(s) 344, 395, 407, 557
AjnI CCWGG 2 cut(s) 237, 887
AluBI AGCT 5 cut(s) 162, 358, 590, 605, 834
AluI AGCT 5 cut(s) 162, 358, 590, 605, 834
Alw21I GWGCWC 1 cut(s) 298
Alw26I GTCTC 5 cut(s) 183, 451, 738, 784, 931
AlwI GGATC 2 cut(s) 294, 619
Ama87I CYCGRG 1 cut(s) 180
AoxI GGCC 5 cut(s) 502, 666, 712, 743, 885
ApeKI GCWGC 5 cut(s) 170, 323, 355, 656, 738
ApoI RAATTY 1 cut(s) 892
ArsI GACNNNNNNTTYG 2 cut(s) 68, 100
AspLEI GCGC 2 cut(s) 465, 467
AspS9I GGNCC 2 cut(s) 316, 502
AsuHPI GGTGA 2 cut(s) 500, 841
AsuNHI GCTAGC 1 cut(s) 4
AvaI CYCGRG 1 cut(s) 180
AvaII GGWCC 1 cut(s) 316
BalI TGGCCA 1 cut(s) 668
Bbv12I GWGCWC 1 cut(s) 298
BbvI GCAGC 5 cut(s) 157, 335, 367, 643, 725
BccI CCATC 3 cut(s) 257, 555, 658
BciT130I CCWGG 2 cut(s) 239, 889
BclI TGATCA 1 cut(s) 897
BcoDI GTCTC 5 cut(s) 183, 451, 738, 784, 931
BfaI CTAG 3 cut(s) 5, 290, 423
BfmI CTRYAG 1 cut(s) 803
BglI GCCNNNNNGGC 2 cut(s) 434, 665
BisI GCNGC 5 cut(s) 171, 324, 356, 657, 739
BlsI GCNGC 5 cut(s) 172, 325, 357, 658, 740
Bme1390I CCNGG 2 cut(s) 239, 889
Bme18I GGWCC 1 cut(s) 316
BmeT110I CYCGRG 1 cut(s) 180
BmgT120I GGNCC 2 cut(s) 316, 502
BmiI GGNNCC 1 cut(s) 318
BmrFI CCNGG 2 cut(s) 239, 889
BmsI GCATC 6 cut(s) 266, 460, 468, 676, 731, 954
BmtI GCTAGC 1 cut(s) 8
BpuEI CTTGAG 1 cut(s) 866
BsaHI GRCGYC 1 cut(s) 793
BsaI GGTCTC 1 cut(s) 183
BsaXI ACNNNNNCTCC 2 cut(s) 258, 288
Bsc4I CCNNNNNNNGG 2 cut(s) 608, 808
Bse118I RCCGGY 1 cut(s) 741
Bse1I ACTGG 3 cut(s) 312, 368, 505
Bse3DI GCAATG 3 cut(s) 192, 201, 276
BseBI CCWGG 2 cut(s) 239, 889
BseGI GGATG 3 cut(s) 249, 847, 945
BseLI CCNNNNNNNGG 2 cut(s) 608, 808
BseMI GCAATG 3 cut(s) 192, 201, 276
BseMII CTCAG 5 cut(s) 311, 539, 547, 648, 942
BseNI ACTGG 3 cut(s) 312, 368, 505
BsePI GCGCGC 1 cut(s) 463
BseXI GCAGC 5 cut(s) 157, 335, 367, 643, 725
BseYI CCCAGC 2 cut(s) 319, 327
BsgI GTGCAG 1 cut(s) 156
Bsh1236I CGCG 1 cut(s) 465
BshFI GGCC 5 cut(s) 504, 668, 714, 745, 887
BsiHKAI GWGCWC 1 cut(s) 298
BsiHKCI CYCGRG 1 cut(s) 180
BsiSI CCGG 1 cut(s) 742
BslFI GGGAC 1 cut(s) 302
BslI CCNNNNNNNGG 2 cut(s) 608, 808
BsmAI GTCTC 5 cut(s) 183, 451, 738, 784, 931
BsmBI CGTCTC 1 cut(s) 784
BsmFI GGGAC 1 cut(s) 302
BsnI GGCC 5 cut(s) 504, 668, 714, 745, 887
Bso31I GGTCTC 1 cut(s) 183
BsoBI CYCGRG 1 cut(s) 180
Bsp1286I GDGCHC 1 cut(s) 298
Bsp143I GATC 5 cut(s) 45, 246, 286, 611, 897
BspACI CCGC 1 cut(s) 137
BspANI GGCC 5 cut(s) 504, 668, 714, 745, 887
BspCNI CTCAG 5 cut(s) 310, 540, 546, 647, 941
BspFNI CGCG 1 cut(s) 465
BspHI TCATGA 1 cut(s) 583
BspLI GGNNCC 1 cut(s) 318
BspOI GCTAGC 1 cut(s) 8
BspPI GGATC 2 cut(s) 294, 619
BspTNI GGTCTC 1 cut(s) 183
BsrDI GCAATG 3 cut(s) 192, 201, 276
BsrFI RCCGGY 1 cut(s) 741
BsrI ACTGG 3 cut(s) 312, 368, 505
BssAI RCCGGY 1 cut(s) 741
BssHII GCGCGC 1 cut(s) 463
BssMI GATC 5 cut(s) 45, 246, 286, 611, 897
BssNI GRCGYC 1 cut(s) 793
Bst2UI CCWGG 2 cut(s) 239, 889
Bst4CI ACNGT 3 cut(s) 177, 629, 807
Bst6I CTCTTC 2 cut(s) 237, 567
BstACI GRCGYC 1 cut(s) 793
BstC8I GCNNGC 3 cut(s) 6, 465, 743
BstDEI CTNAG 5 cut(s) 297, 533, 548, 634, 928
BstF5I GGATG 3 cut(s) 249, 847, 945
BstFNI CGCG 1 cut(s) 465
BstHHI GCGC 2 cut(s) 465, 467
BstKTI GATC 5 cut(s) 48, 249, 289, 614, 900
BstMAI GTCTC 5 cut(s) 183, 451, 738, 784, 931
BstMBI GATC 5 cut(s) 45, 246, 286, 611, 897
BstMWI GCNNNNNNNGC 5 cut(s) 203, 275, 364, 434, 665
BstNI CCWGG 2 cut(s) 239, 889
BstNSI RCATGY 1 cut(s) 18
BstSCI CCNGG 2 cut(s) 237, 887
BstSFI CTRYAG 1 cut(s) 803
BstUI CGCG 1 cut(s) 465
BstV1I GCAGC 5 cut(s) 157, 335, 367, 643, 725
BstX2I RGATCY 2 cut(s) 286, 611
BstXI CCANNNNNNTGG 1 cut(s) 676
BstYI RGATCY 2 cut(s) 286, 611
BsuRI GGCC 5 cut(s) 504, 668, 714, 745, 887
BtsCI GGATG 3 cut(s) 249, 847, 945
BtsI GCAGTG 4 cut(s) 15, 459, 875, 947
BtsIMutI CAGTG 6 cut(s) 15, 305, 459, 812, 875, 947
Cac8I GCNNGC 3 cut(s) 6, 465, 743
CciI TCATGA 1 cut(s) 583
CfoI GCGC 2 cut(s) 465, 467
Cfr10I RCCGGY 1 cut(s) 741
Cfr13I GGNCC 2 cut(s) 316, 502
CseI GACGC 1 cut(s) 801
Csp6I GTAC 1 cut(s) 785
CviAII CATG 4 cut(s) 15, 584, 718, 847
CviQI GTAC 1 cut(s) 785
DdeI CTNAG 5 cut(s) 297, 533, 548, 634, 928
DpnI GATC 5 cut(s) 47, 248, 288, 613, 899
DpnII GATC 5 cut(s) 45, 246, 286, 611, 897
DrdI GACNNNNNNGTC 1 cut(s) 197
DseDI GACNNNNNNGTC 1 cut(s) 197
EaeI YGGCCR 2 cut(s) 666, 743
Eam1104I CTCTTC 2 cut(s) 237, 567
EarI CTCTTC 2 cut(s) 237, 567
Eco31I GGTCTC 1 cut(s) 183
Eco47I GGWCC 1 cut(s) 316
Eco57I CTGAAG 1 cut(s) 468
Eco88I CYCGRG 1 cut(s) 180
EcoO109I RGGNCCY 1 cut(s) 316
EcoRII CCWGG 2 cut(s) 237, 887
EcoT22I ATGCAT 1 cut(s) 20
Esp3I CGTCTC 1 cut(s) 784
FaeI CATG 4 cut(s) 18, 587, 721, 850
FaqI GGGAC 1 cut(s) 302
FatI CATG 4 cut(s) 14, 583, 717, 846
FbaI TGATCA 1 cut(s) 897
Fnu4HI GCNGC 5 cut(s) 171, 324, 356, 657, 739
FokI GGATG 3 cut(s) 236, 854, 932
Fsp4HI GCNGC 5 cut(s) 171, 324, 356, 657, 739
FspBI CTAG 3 cut(s) 5, 290, 423
GlaI GCGC 2 cut(s) 464, 466
GluI GCNGC 5 cut(s) 171, 324, 356, 657, 739
GsaI CCCAGC 2 cut(s) 323, 331
HaeIII GGCC 5 cut(s) 504, 668, 714, 745, 887
HapII CCGG 1 cut(s) 742
HgaI GACGC 1 cut(s) 801
HhaI GCGC 2 cut(s) 465, 467
Hin1I GRCGYC 1 cut(s) 793
Hin1II CATG 4 cut(s) 18, 587, 721, 850
Hin6I GCGC 2 cut(s) 463, 465
HinP1I GCGC 2 cut(s) 463, 465
HinfI GANTC 3 cut(s) 224, 395, 491
HpaII CCGG 1 cut(s) 742
HphI GGTGA 2 cut(s) 500, 841
Hpy188III TCNNGA 3 cut(s) 180, 182, 584
HpyCH4III ACNGT 3 cut(s) 177, 629, 807
HpyCH4IV ACGT 1 cut(s) 580
HpyCH4V TGCA 7 cut(s) 18, 95, 173, 206, 648, 940, 945
HpyF10VI GCNNNNNNNGC 5 cut(s) 203, 275, 364, 434, 665
HpyF3I CTNAG 5 cut(s) 297, 533, 548, 634, 928
HpySE526I ACGT 1 cut(s) 580
Hsp92I GRCGYC 1 cut(s) 793
Hsp92II CATG 4 cut(s) 18, 587, 721, 850
HspAI GCGC 2 cut(s) 463, 465
KroI GCCGGC 1 cut(s) 741
KroNI GCCGGC 1 cut(s) 743
Ksp22I TGATCA 1 cut(s) 897
Kzo9I GATC 5 cut(s) 45, 246, 286, 611, 897
LmnI GCTCC 1 cut(s) 266
Lsp1109I GCAGC 5 cut(s) 157, 335, 367, 643, 725
LweI GCATC 6 cut(s) 266, 460, 468, 676, 731, 954
MaeI CTAG 3 cut(s) 5, 290, 423
MaeII ACGT 1 cut(s) 580
MaeIII GTNAC 2 cut(s) 10, 873
MalI GATC 5 cut(s) 47, 248, 288, 613, 899
MboI GATC 5 cut(s) 45, 246, 286, 611, 897
MboII GAAGA 3 cut(s) 254, 584, 606
MflI RGATCY 2 cut(s) 286, 611
MhlI GDGCHC 1 cut(s) 298
MlsI TGGCCA 1 cut(s) 668
MluCI AATT 4 cut(s) 386, 552, 673, 892
MluNI TGGCCA 1 cut(s) 668
MlyI GAGTC 2 cut(s) 218, 500
MmeI TCCRAC 1 cut(s) 54
MnlI CCTC 4 cut(s) 91, 275, 561, 673
Mox20I TGGCCA 1 cut(s) 668
Mph1103I ATGCAT 1 cut(s) 20
MroNI GCCGGC 1 cut(s) 741
MscI TGGCCA 1 cut(s) 668
MseI TTAA 2 cut(s) 156, 861
Msp20I TGGCCA 1 cut(s) 668
MspI CCGG 1 cut(s) 742
MspR9I CCNGG 2 cut(s) 239, 889
MvaI CCWGG 2 cut(s) 239, 889
MvnI CGCG 1 cut(s) 465
MwoI GCNNNNNNNGC 5 cut(s) 203, 275, 364, 434, 665
NaeI GCCGGC 1 cut(s) 743
NdeII GATC 5 cut(s) 45, 246, 286, 611, 897
NgoMIV GCCGGC 1 cut(s) 741
NheI GCTAGC 1 cut(s) 4
NlaIII CATG 4 cut(s) 18, 587, 721, 850
NlaIV GGNNCC 1 cut(s) 318
NmuCI GTSAC 2 cut(s) 10, 873
NsiI ATGCAT 1 cut(s) 20
NspI RCATGY 1 cut(s) 18
PaeR7I CTCGAG 1 cut(s) 180
PagI TCATGA 1 cut(s) 583
PauI GCGCGC 1 cut(s) 463
PdiI GCCGGC 1 cut(s) 743
PfeI GAWTC 1 cut(s) 395
PflMI CCANNNNNTGG 1 cut(s) 608
PkrI GCNGC 5 cut(s) 172, 325, 357, 658, 740
PleI GAGTC 2 cut(s) 218, 499
PpsI GAGTC 2 cut(s) 218, 499
PpuMI RGGWCCY 1 cut(s) 316
Psp5II RGGWCCY 1 cut(s) 316
Psp6I CCWGG 2 cut(s) 237, 887
PspFI CCCAGC 2 cut(s) 319, 327
PspGI CCWGG 2 cut(s) 237, 887
PspN4I GGNNCC 1 cut(s) 318
PspPI GGNCC 2 cut(s) 316, 502
PspPPI RGGWCCY 1 cut(s) 316
PsuI RGATCY 2 cut(s) 286, 611
PteI GCGCGC 1 cut(s) 463
RsaI GTAC 1 cut(s) 786
RsaNI GTAC 1 cut(s) 785
SaqAI TTAA 2 cut(s) 156, 861
SatI GCNGC 5 cut(s) 171, 324, 356, 657, 739
Sau3AI GATC 5 cut(s) 45, 246, 286, 611, 897
Sau96I GGNCC 2 cut(s) 316, 502
SchI GAGTC 2 cut(s) 218, 500
ScrFI CCNGG 2 cut(s) 239, 889
SduI GDGCHC 1 cut(s) 298
SfaNI GCATC 6 cut(s) 266, 460, 468, 676, 731, 954
SfcI CTRYAG 1 cut(s) 803
Sfr274I CTCGAG 1 cut(s) 180
SinI GGWCC 1 cut(s) 316
SlaI CTCGAG 1 cut(s) 180
SmlI CTYRAG 2 cut(s) 180, 881
SmoI CTYRAG 2 cut(s) 180, 881
Sse9I AATT 4 cut(s) 386, 552, 673, 892
SsiI CCGC 1 cut(s) 137
SspMI CTAG 3 cut(s) 5, 290, 423
StyD4I CCNGG 2 cut(s) 237, 887
TaaI ACNGT 3 cut(s) 177, 629, 807
TaiI ACGT 1 cut(s) 583
TaqI TCGA 1 cut(s) 181
TasI AATT 4 cut(s) 386, 552, 673, 892
TfiI GAWTC 1 cut(s) 395
Tru1I TTAA 2 cut(s) 156, 861
Tru9I TTAA 2 cut(s) 156, 861
TscAI CASTG 6 cut(s) 15, 312, 459, 812, 882, 947
TseFI GTSAC 2 cut(s) 10, 873
TseI GCWGC 5 cut(s) 170, 323, 355, 656, 738
Tsp45I GTSAC 2 cut(s) 10, 873
TspDTI ATGAA 3 cut(s) 24, 600, 606
TspGWI ACGGA 1 cut(s) 802
TspRI CASTG 6 cut(s) 15, 312, 459, 812, 882, 947
Van91I CCANNNNNTGG 1 cut(s) 608
VpaK11BI GGWCC 1 cut(s) 316
XapI RAATTY 1 cut(s) 892
XceI RCATGY 1 cut(s) 18
XhoI CTCGAG 1 cut(s) 180
XspI CTAG 3 cut(s) 5, 290, 423
Zsp2I ATGCAT 1 cut(s) 20
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.