MD13G1202500.v1.1

Belongs to the pyruvate kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0009431
Physical Location & Seq
Forward (+)
18150402 .. 18152556
2155 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1202500.v1.1.491

Sequence Viewer

Length: 999 bp
ATGTCGCAGTCCATACATCTCCTCACCCCGTCCAACCTCGCCATCTCAAAGCACCACTCCTCCTTCCTCTCCACCTTCAATCGCCGGTTGCCGGTCATCACCACCACCACCAAGACCACCGCGCCTTCTTTCCCAAAGCTCTCCATCAGAGCCTCCTCCTCCGATCGCGAGCCTTCCGTTCTCGTCACCGACAACGGCAAGTCCTCCTCGGGGCTCCAATCCCCTCCTTCGCACTCCGACCATGCTCTTTCCAGCTCCATCGAGGTCGACGCCGTCACCGAAGCTGAGCTCCGGGAGAACGGATTCCGTTCCACCAGGCGCACCAAGCTCGTATGCACCATCGGACCCGCCACCTCCGGATTCGACCAGCTCGAATCTCTCGCCGTTGGCGGCATGAACGTAGCGCGCATCAACATGTGCCACGGCACGCGCGAGTGGCACCGCGAGGTCATTCAGCGCGTCAGGAGGCTCAACGAGGACAAGGGATACGCCGTCGCCATCATGATGGATACCGAAGGCAGCGAGATTCATATGGGTGATTTGGGTGGCGCTTCCTCCGCCAAAGCTGAGGATGGTGAAGTATGGACTTTCAGCGTCAGAGCTTTCGGCTCCACTCTGCCCGAACACACCATCAATGTGAACTATGAAGGTTTTGCCGAATTGAAATGGCGAAAATTGATGGATGATATGATGCTTGCAGATGTGAAAGTAGGGGATGAGCTGCTTGTGGATGGTGGGATGGTGAGATTCGAGGTGATCGAAAAGCTTGGTCCTGATGTTAAGTGTAAGTGTACTGATCCTGGTTTGTTGCTGCCTCGGGCTAATTTGACTTTCTGGAGGGACGGGAGTTTAGTGCGAGAACGTAATGCCATGCTTCCCACCATTTCGTCGAAGTCCTCTTTGATAACTGTATTATTGATTGATAAGCATGGAGCTTTGGTTTCCGACTCCAGAGCGTTAGTGTCTTCTGATACGAAGCATCCTTGCCTACAGATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000287 GO:0003006 GO:0003674 GO:0003824 GO:0004743 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006629 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009791 GO:0009987 GO:0010035 GO:0010038 GO:0010154 GO:0010431 GO:0016052 GO:0016053 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0021700 GO:0022414 GO:0030955 GO:0031420 GO:0032501 GO:0032502 GO:0032504 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042221 GO:0042866 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046686 GO:0046700 GO:0046872 GO:0046939 GO:0048316 GO:0048608 GO:0048609 GO:0048731 GO:0048856 GO:0050896 GO:0051186 GO:0051188 GO:0055086 GO:0061458 GO:0071695 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00230 ko00620 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko04922 ko04930 ko05165 ko05203 ko05230 map00010 map00230 map00620 map01100 map01110 map01120 map01130 map01200 map01230 map04922 map04930 map05165 map05203 map05230
Pfam Domains
Protein Families
PK PK_C

Protein Analysis

333

Amino Acids

36.32

Weight (kDa)

6.6

Isoelectric Point (pI)

45.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK PF00224 106 - 273 8.5e-28 Pyruvate kinase, barrel domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 438
AccI GTMKAC 1 cut(s) 267
AccII CGCG 7 cut(s) 122, 168, 406, 430, 432, 444, 459
AccIII TCCGGA 1 cut(s) 356
AciI CCGC 5 cut(s) 120, 348, 390, 442, 558
AclWI GGATC 1 cut(s) 791
AcyI GRCGYC 1 cut(s) 270
AfaI GTAC 1 cut(s) 793
AfiI CCNNNNNNNGG 2 cut(s) 91, 210
AflIII ACRYGT 1 cut(s) 414
AgsI TTSAA 2 cut(s) 79, 664
AjnI CCWGG 2 cut(s) 314, 799
Alw21I GWGCWC 1 cut(s) 291
AlwI GGATC 1 cut(s) 791
Ama87I CYCGRG 2 cut(s) 208, 816
Aor13HI TCCGGA 1 cut(s) 356
ApeKI GCWGC 3 cut(s) 519, 721, 811
Asp700I GAANNNNTTC 1 cut(s) 302
AspLEI GCGC 7 cut(s) 124, 321, 406, 408, 432, 459, 551
AspS9I GGNCC 2 cut(s) 344, 770
AsuC2I CCSGG 1 cut(s) 293
AsuHPI GGTGA 8 cut(s) 16, 91, 178, 268, 548, 587, 754, 766
AvaI CYCGRG 2 cut(s) 208, 816
AvaII GGWCC 2 cut(s) 344, 770
BanI GGYRCC 1 cut(s) 438
BanII GRGCYC 2 cut(s) 216, 291
BbsI GAAGAC 1 cut(s) 957
Bbv12I GWGCWC 1 cut(s) 291
BbvCI CCTCAGC 1 cut(s) 567
BbvI GCAGC 3 cut(s) 531, 708, 798
BceAI ACGGC 5 cut(s) 211, 257, 368, 439, 476
BcgI CGANNNNNNTGC 2 cut(s) 310, 344
BciT130I CCWGG 2 cut(s) 316, 801
BciVI GTATCC 2 cut(s) 479, 502
BcnI CCSGG 1 cut(s) 293
BfmI CTRYAG 1 cut(s) 989
BfoI RGCGCY 1 cut(s) 552
BfuI GTATCC 2 cut(s) 479, 502
BisI GCNGC 4 cut(s) 391, 520, 722, 812
BlpI GCTNAGC 1 cut(s) 285
BlsI GCNGC 4 cut(s) 392, 521, 723, 813
Bme1390I CCNGG 3 cut(s) 293, 316, 801
Bme18I GGWCC 2 cut(s) 344, 770
BmeT110I CYCGRG 2 cut(s) 208, 816
BmgT120I GGNCC 2 cut(s) 344, 770
BmiI GGNNCC 4 cut(s) 215, 346, 440, 610
BmrFI CCNGG 3 cut(s) 293, 316, 801
BmsI GCATC 3 cut(s) 417, 681, 988
BpiI GAAGAC 1 cut(s) 957
BpmI CTGGAG 2 cut(s) 856, 934
Bpu10I CCTNAGC 1 cut(s) 567
Bpu1102I GCTNAGC 1 cut(s) 285
BpuMI CCSGG 1 cut(s) 293
BsaHI GRCGYC 1 cut(s) 270
BsaJI CCNNGG 3 cut(s) 207, 421, 815
BsaWI WCCGGW 1 cut(s) 356
BsaXI ACNNNNNCTCC 4 cut(s) 44, 74, 924, 954
Bsc4I CCNNNNNNNGG 2 cut(s) 91, 210
Bse118I RCCGGY 2 cut(s) 84, 91
BseAI TCCGGA 1 cut(s) 356
BseBI CCWGG 2 cut(s) 316, 801
BseDI CCNNGG 3 cut(s) 207, 421, 815
BseGI GGATG 6 cut(s) 577, 688, 721, 736, 744, 979
BseLI CCNNNNNNNGG 2 cut(s) 91, 210
BseMII CTCAG 2 cut(s) 276, 558
BsePI GCGCGC 1 cut(s) 404
BseRI GAGGAG 5 cut(s) 11, 49, 145, 148, 196
BseXI GCAGC 3 cut(s) 531, 708, 798
Bsh1236I CGCG 7 cut(s) 122, 168, 406, 430, 432, 444, 459
Bsh1285I CGRYCG 1 cut(s) 166
BshNI GGYRCC 1 cut(s) 438
BsiEI CGRYCG 1 cut(s) 166
BsiHKAI GWGCWC 1 cut(s) 291
BsiHKCI CYCGRG 2 cut(s) 208, 816
BsiSI CCGG 4 cut(s) 85, 92, 292, 357
BslFI GGGAC 1 cut(s) 854
BslI CCNNNNNNNGG 2 cut(s) 91, 210
BsmFI GGGAC 1 cut(s) 854
BsoBI CYCGRG 2 cut(s) 208, 816
Bsp1286I GDGCHC 2 cut(s) 216, 291
Bsp13I TCCGGA 1 cut(s) 356
Bsp143I GATC 3 cut(s) 163, 756, 796
Bsp1720I GCTNAGC 1 cut(s) 285
Bsp68I TCGCGA 1 cut(s) 168
BspACI CCGC 5 cut(s) 120, 348, 390, 442, 558
BspCNI CTCAG 2 cut(s) 277, 559
BspEI TCCGGA 1 cut(s) 356
BspFNI CGCG 7 cut(s) 122, 168, 406, 430, 432, 444, 459
BspHI TCATGA 1 cut(s) 501
BspLI GGNNCC 4 cut(s) 215, 346, 440, 610
BspPI GGATC 1 cut(s) 791
BspT107I GGYRCC 1 cut(s) 438
BsrFI RCCGGY 2 cut(s) 84, 91
BssAI RCCGGY 2 cut(s) 84, 91
BssECI CCNNGG 3 cut(s) 207, 421, 815
BssHII GCGCGC 1 cut(s) 404
BssMI GATC 3 cut(s) 163, 756, 796
BssNI GRCGYC 1 cut(s) 270
Bst2UI CCWGG 2 cut(s) 316, 801
Bst4CI ACNGT 1 cut(s) 910
BstACI GRCGYC 1 cut(s) 270
BstC8I GCNNGC 4 cut(s) 170, 406, 428, 696
BstDEI CTNAG 2 cut(s) 285, 567
BstDSI CCRYGG 1 cut(s) 421
BstF5I GGATG 6 cut(s) 577, 688, 721, 736, 744, 979
BstFNI CGCG 7 cut(s) 122, 168, 406, 430, 432, 444, 459
BstH2I RGCGCY 1 cut(s) 552
BstHHI GCGC 7 cut(s) 124, 321, 406, 408, 432, 459, 551
BstKTI GATC 3 cut(s) 166, 759, 799
BstMBI GATC 3 cut(s) 163, 756, 796
BstMCI CGRYCG 1 cut(s) 166
BstMWI GCNNNNNNNGC 3 cut(s) 325, 436, 557
BstNI CCWGG 2 cut(s) 316, 801
BstNSI RCATGY 1 cut(s) 418
BstSCI CCNGG 3 cut(s) 291, 314, 799
BstSFI CTRYAG 1 cut(s) 989
BstUI CGCG 7 cut(s) 122, 168, 406, 430, 432, 444, 459
BstV1I GCAGC 3 cut(s) 531, 708, 798
BstV2I GAAGAC 1 cut(s) 957
BstXI CCANNNNNNTGG 1 cut(s) 505
BsuI GTATCC 2 cut(s) 479, 502
BtgI CCRYGG 1 cut(s) 421
BtsCI GGATG 6 cut(s) 577, 688, 721, 736, 744, 979
BtuMI TCGCGA 1 cut(s) 168
Cac8I GCNNGC 4 cut(s) 170, 406, 428, 696
CciI TCATGA 1 cut(s) 501
CfoI GCGC 7 cut(s) 124, 321, 406, 408, 432, 459, 551
Cfr10I RCCGGY 2 cut(s) 84, 91
Cfr13I GGNCC 2 cut(s) 344, 770
CseI GACGC 3 cut(s) 278, 448, 583
Csp6I GTAC 1 cut(s) 792
CviAII CATG 6 cut(s) 242, 394, 415, 502, 871, 929
CviQI GTAC 1 cut(s) 792
DdeI CTNAG 2 cut(s) 285, 567
DpnI GATC 3 cut(s) 165, 758, 798
DpnII GATC 3 cut(s) 163, 756, 796
EciI GGCGGA 1 cut(s) 547
Ecl136II GAGCTC 1 cut(s) 289
Eco24I GRGCYC 2 cut(s) 216, 291
Eco47I GGWCC 2 cut(s) 344, 770
Eco53kI GAGCTC 1 cut(s) 289
Eco88I CYCGRG 2 cut(s) 208, 816
EcoICRI GAGCTC 1 cut(s) 289
EcoRII CCWGG 2 cut(s) 314, 799
EcoT38I GRGCYC 2 cut(s) 216, 291
FaeI CATG 6 cut(s) 245, 397, 418, 505, 874, 932
FaqI GGGAC 1 cut(s) 854
FatI CATG 6 cut(s) 241, 393, 414, 501, 870, 928
FauI CCCGC 1 cut(s) 355
FauNDI CATATG 1 cut(s) 531
FblI GTMKAC 1 cut(s) 267
Fnu4HI GCNGC 4 cut(s) 391, 520, 722, 812
FokI GGATG 6 cut(s) 584, 695, 728, 743, 751, 966
FriOI GRGCYC 2 cut(s) 216, 291
Fsp4HI GCNGC 4 cut(s) 391, 520, 722, 812
GlaI GCGC 7 cut(s) 123, 320, 405, 407, 431, 458, 550
GluI GCNGC 4 cut(s) 391, 520, 722, 812
GsuI CTGGAG 2 cut(s) 856, 934
HaeII RGCGCY 1 cut(s) 552
HapII CCGG 4 cut(s) 85, 92, 292, 357
HgaI GACGC 3 cut(s) 278, 448, 583
HhaI GCGC 7 cut(s) 124, 321, 406, 408, 432, 459, 551
Hin1I GRCGYC 1 cut(s) 270
Hin1II CATG 6 cut(s) 245, 397, 418, 505, 874, 932
Hin6I GCGC 7 cut(s) 122, 319, 404, 406, 430, 457, 549
HinP1I GCGC 7 cut(s) 122, 319, 404, 406, 430, 457, 549
HincII GTYRAC 1 cut(s) 268
HindII GTYRAC 1 cut(s) 268
HindIII AAGCTT 1 cut(s) 764
HinfI GANTC 6 cut(s) 303, 360, 374, 526, 747, 947
HpaII CCGG 4 cut(s) 85, 92, 292, 357
HphI GGTGA 8 cut(s) 16, 91, 178, 268, 548, 587, 754, 766
Hpy166II GTNNAC 3 cut(s) 268, 640, 792
Hpy188I TCNGA 7 cut(s) 149, 163, 238, 344, 599, 946, 970
Hpy188III TCNNGA 7 cut(s) 167, 357, 463, 502, 773, 835, 951
Hpy8I GTNNAC 3 cut(s) 268, 640, 792
Hpy99I CGWCG 3 cut(s) 272, 497, 892
HpyAV CCTTC 7 cut(s) 73, 85, 135, 183, 237, 509, 641
HpyCH4III ACNGT 1 cut(s) 910
HpyCH4IV ACGT 2 cut(s) 399, 862
HpyCH4V TGCA 2 cut(s) 336, 698
HpyF10VI GCNNNNNNNGC 3 cut(s) 325, 436, 557
HpyF3I CTNAG 2 cut(s) 285, 567
HpySE526I ACGT 2 cut(s) 399, 862
Hsp92I GRCGYC 1 cut(s) 270
Hsp92II CATG 6 cut(s) 245, 397, 418, 505, 874, 932
HspAI GCGC 7 cut(s) 122, 319, 404, 406, 430, 457, 549
Kpn2I TCCGGA 1 cut(s) 356
Kzo9I GATC 3 cut(s) 163, 756, 796
LmnI GCTCC 5 cut(s) 219, 260, 294, 614, 932
Lsp1109I GCAGC 3 cut(s) 531, 708, 798
LweI GCATC 3 cut(s) 417, 681, 988
MaeII ACGT 2 cut(s) 399, 862
MaeIII GTNAC 2 cut(s) 184, 274
MalI GATC 3 cut(s) 165, 758, 798
MboI GATC 3 cut(s) 163, 756, 796
MboII GAAGA 1 cut(s) 957
MhlI GDGCHC 2 cut(s) 216, 291
MluCI AATT 3 cut(s) 659, 674, 823
MlyI GAGTC 1 cut(s) 941
MmeI TCCRAC 3 cut(s) 57, 261, 969
MroI TCCGGA 1 cut(s) 356
MroXI GAANNNNTTC 1 cut(s) 302
MseI TTAA 1 cut(s) 780
MslI CAYNNNNRTG 4 cut(s) 413, 503, 534, 635
MspI CCGG 4 cut(s) 85, 92, 292, 357
MspR9I CCNGG 3 cut(s) 293, 316, 801
MvaI CCWGG 2 cut(s) 316, 801
MvnI CGCG 7 cut(s) 122, 168, 406, 430, 432, 444, 459
MwoI GCNNNNNNNGC 3 cut(s) 325, 436, 557
NciI CCSGG 1 cut(s) 293
NdeI CATATG 1 cut(s) 531
NdeII GATC 3 cut(s) 163, 756, 796
NlaIII CATG 6 cut(s) 245, 397, 418, 505, 874, 932
NlaIV GGNNCC 4 cut(s) 215, 346, 440, 610
NmuCI GTSAC 2 cut(s) 184, 274
NruI TCGCGA 1 cut(s) 168
NspI RCATGY 1 cut(s) 418
PagI TCATGA 1 cut(s) 501
PauI GCGCGC 1 cut(s) 404
PciI ACATGT 1 cut(s) 414
PcsI WCGNNNNNNNCGW 4 cut(s) 276, 369, 429, 756
PdmI GAANNNNTTC 1 cut(s) 302
PfeI GAWTC 5 cut(s) 303, 360, 374, 526, 747
PflFI GACNNNGTC 1 cut(s) 272
PfoI TCCNGGA 1 cut(s) 291
PkrI GCNGC 4 cut(s) 392, 521, 723, 813
Ple19I CGATCG 1 cut(s) 166
PleI GAGTC 1 cut(s) 941
PpsI GAGTC 1 cut(s) 941
PscI ACATGT 1 cut(s) 414
Psp124BI GAGCTC 1 cut(s) 291
Psp6I CCWGG 2 cut(s) 314, 799
PspGI CCWGG 2 cut(s) 314, 799
PspN4I GGNNCC 4 cut(s) 215, 346, 440, 610
PspPI GGNCC 2 cut(s) 344, 770
PsyI GACNNNGTC 1 cut(s) 272
PteI GCGCGC 1 cut(s) 404
PvuI CGATCG 1 cut(s) 166
RruI TCGCGA 1 cut(s) 168
RsaI GTAC 1 cut(s) 793
RsaNI GTAC 1 cut(s) 792
RseI CAYNNNNRTG 4 cut(s) 413, 503, 534, 635
SacI GAGCTC 1 cut(s) 291
SalI GTCGAC 1 cut(s) 266
SaqAI TTAA 1 cut(s) 780
SatI GCNGC 4 cut(s) 391, 520, 722, 812
Sau3AI GATC 3 cut(s) 163, 756, 796
Sau96I GGNCC 2 cut(s) 344, 770
SchI GAGTC 1 cut(s) 941
ScrFI CCNGG 3 cut(s) 293, 316, 801
SduI GDGCHC 2 cut(s) 216, 291
SfaNI GCATC 3 cut(s) 417, 681, 988
SfcI CTRYAG 1 cut(s) 989
SinI GGWCC 2 cut(s) 344, 770
SmiMI CAYNNNNRTG 4 cut(s) 413, 503, 534, 635
Sse9I AATT 3 cut(s) 659, 674, 823
SsiI CCGC 5 cut(s) 120, 348, 390, 442, 558
SstI GAGCTC 1 cut(s) 291
StyD4I CCNGG 3 cut(s) 291, 314, 799
TaaI ACNGT 1 cut(s) 910
TaiI ACGT 2 cut(s) 402, 865
TaqI TCGA 7 cut(s) 261, 267, 363, 372, 750, 759, 890
TasI AATT 3 cut(s) 659, 674, 823
TatI WGTACW 1 cut(s) 791
TauI GCSGC 1 cut(s) 393
TfiI GAWTC 5 cut(s) 303, 360, 374, 526, 747
Tru1I TTAA 1 cut(s) 780
Tru9I TTAA 1 cut(s) 780
TseFI GTSAC 2 cut(s) 184, 274
TseI GCWGC 3 cut(s) 519, 721, 811
Tsp45I GTSAC 2 cut(s) 184, 274
TspDTI ATGAA 3 cut(s) 410, 518, 660
TspGWI ACGGA 3 cut(s) 166, 296, 315
Tth111I GACNNNGTC 1 cut(s) 272
VpaK11BI GGWCC 2 cut(s) 344, 770
XceI RCATGY 1 cut(s) 418
XmiI GTMKAC 1 cut(s) 267
XmnI GAANNNNTTC 1 cut(s) 302
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.